CN104764851B - The assay method of 16 kinds of palycyclic aromatics in a kind of tobacco juice for electronic smoke - Google Patents
The assay method of 16 kinds of palycyclic aromatics in a kind of tobacco juice for electronic smoke Download PDFInfo
- Publication number
- CN104764851B CN104764851B CN201510233951.8A CN201510233951A CN104764851B CN 104764851 B CN104764851 B CN 104764851B CN 201510233951 A CN201510233951 A CN 201510233951A CN 104764851 B CN104764851 B CN 104764851B
- Authority
- CN
- China
- Prior art keywords
- kinds
- benzo
- palycyclic
- sample
- tobacco juice
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The assay method of 16 kinds of palycyclic aromatics in a kind of tobacco juice for electronic smoke, it is characterized in that: the method adds a small amount of water by tobacco juice for electronic smoke sample, shaken well, being well dispersed in zeyssatite solid phase supports in liquid-liquid extraction post, extract with cyclohexane, after extract is concentrated, gas chromatograph-mass spectrometer (GCMS) (GC-MS) is adopted to realize the mensuration of 16 kinds of palycyclic aromatics in tobacco juice for electronic smoke.Analysis determining method of the present invention, compared with measuring the common analysis of palycyclic aromatic at present, enormously simplify sample pre-treatments, has saved organic solvent use, and the repeatability of experiment and the recovery very well, are applicable to robotization and mass disposal sample.
Description
Technical field
The invention belongs to tobacco juice for electronic smoke determination techniques field, be specifically related to the assay method of 16 kinds of palycyclic aromatics in a kind of tobacco juice for electronic smoke.The method adds a small amount of water by tobacco juice for electronic smoke sample, shaken well, is well dispersed in zeyssatite solid phase and supports, in liquid-liquid extraction post, to extract with cyclohexane, after extract is concentrated, gas chromatograph-mass spectrometer (GCMS) (GC-MS) is adopted to realize the mensuration of 16 kinds of palycyclic aromatics in tobacco juice for electronic smoke.
Background technology
Electronic cigarette (electricalcigarette, e-cigarette), have another name called electronics nicotine transfer system (electronicnicotinedeliverysystems, ENDS), being a kind of electronic installation being transmitted nicotine by electrons heat means to respiratory system, is a kind of new paragon sucking nicotine.Tobacco juice for electronic smoke is formed primarily of nicotine, propylene glycol, glycerine or polyglycol, can add a small amount of essence in addition, make different tastes to cater to consumer demand and hobby.
Palycyclic aromatic is the staying quality poison material of a quasi-representative, has carcinogenic, teratogenesis and mutagenicity.The Polycyclic aromatic hydrocarbons and Derivatives found at present is more than 400 kinds, and wherein 16 kinds of palycyclic aromatics are classified as the organic contaminant of priority acccess control by Environmental Protection Agency (USEnvironmentalProtectionAgency).These 16 kinds of palycyclic aromatics are common environment and food contaminant, and its carcinogenicity is comparatively strong, even trace, still the health of the mankind in serious threat.But its assay in tobacco juice for electronic smoke, rarely has report.
At present, analyze the pre-treatment detecting palycyclic aromatic and mostly adopt solid phase extraction (Solid-PhaseExtraction), its process is divided into the steps such as activation, drip washing, wash-out, loaded down with trivial details consuming time.Because tobacco juice for electronic smoke matrix is comparatively simple and be mainly polar component, pre-treatment of the present invention adopts solid phase to support liquid-liquid technique (SupportedLiquidExtraction), for a kind of novel liquid-liquid extracting process, it is on the basis of traditional liquid-liquid extraction, be medium by a kind of inertia porous mass-calcined diatomite, when aqueous samples is through later, tripolite filling material surface is distributed in the form of liquid film of thin layer, after adding organic solvent, immiscible two-phase extracts and occurs immediately while zeyssatite surface contact, achieve efficient, extraction process fast.
Summary of the invention
The assay method of 16 kinds of palycyclic aromatics in a kind of tobacco juice for electronic smoke provided for above current condition is provided, the method adopts inner mark method ration, liquid-liquid technique makes sample pre-treatments simplify to utilize solid phase to support, accuracy, reproducible, the recovery is high.
The object of the invention is to be realized by following scheme:
The assay method of 16 kinds of palycyclic aromatics in a kind of tobacco juice for electronic smoke, add a small amount of water by tobacco juice for electronic smoke sample, shaken well, being well dispersed in zeyssatite solid phase supports in liquid-liquid extraction post, extract with cyclohexane, after extract is concentrated, adopt gas chromatograph-mass spectrometer (GCMS) (GC-MS) to realize the mensuration of 16 kinds of palycyclic aromatics in tobacco juice for electronic smoke, concrete determination step is as follows:
(1) extraction of testing sample and concentrated: accurately take 0.1 ~ 3g electronic cigarette liquid in conical flask, add D
8-Nai, D
10-Fei, D
12mark and 0.9 ~ 3mL water in-benzo [a] pyrene 3 kinds, all proceeding to solid phase after vibration 2 ~ 10min supports in liquid-liquid extraction post, pressurization makes testing sample solution fully spread in column packing, leave standstill 2 ~ 10min, add 5 ~ 24mL cyclohexane wash-out, collect eluent, be concentrated into about 0.2 ~ 1mL, detect with GC-MS;
(2) preparation of standard working solution: preparation to have in 16 kinds of palycyclic aromatics of concentration gradient and 3 kinds target cyclohexane solution as hybrid standard working solution;
(3) analysis of sample:
GC-MS analysis condition:
Injector temperature: 280 DEG C; Sample size: 1 μ L; Splitless injecting-Sample; Carrier gas: helium, constant current flow velocity 1mL/min; Chromatographic column: DB-5MS, specification 30m × 0.25mm × 0.25 μm; Temperature programme: 60 DEG C keep 1min, then rise to 110 DEG C with the speed of 20 DEG C/min, then rise to 280 DEG C with the speed of 5 DEG C/min, keep 10min.
Ionization mode: EI; Transmission line temperature: 280 DEG C; Ion source temperature: 230 DEG C.
Scan mode: SIM, Selective ion mode, see table 1, is 50ms to the monitoring time of each ion.
(4) Specification Curve of Increasing and result calculate: carry out area integral to the response of series standard solution, do calibration curve, carry out matching, obtain unary linear regression equation with straight line with the ratio of often kind of palycyclic aromatic and interior target response area comparison content.The sample of preparation is measured, records often kind of palycyclic aromatic in sample and interior mark peak area ratio, bring unary linear regression equation into, calculate often kind of polycyclic aromatic hydrocarbon content.
The preparation method of hybrid standard working solution is as follows:
1) preparation of hybrid standard stock solution: accurately take 10mg16 kind palycyclic aromatic respectively in the brown volumetric flask of 100mL, dissolve constant volume with cyclohexane.The preparation of mark stock solution in mixing: accurately take 10mgD respectively
8-Nai, D
10-Fei, D
12-benzo [a] pyrene, in the brown volumetric flask of 100mL, dissolves constant volume with cyclohexane.
2) preparation of hybrid standard working solution: accurately add 5uL respectively, marks stock solution in 50mL volumetric flask in 10uL, 20uL, 40uL, 80uL hybrid standard stock solution and 40uL mixing, uses cyclohexane constant volume.
In the present invention, the column packing of employing is zeyssatite.
The water adopted is high purity water.
Fully diffusion institute plus-pressure can not more than 10psi in column packing for sample solution.
Before sample solution extraction, keep sample solution in pillar, can not flow out.
When sample solution wash-out, control the eluent rate of outflow and be about 2mL/min(1 drop/sec).
Method of the present invention is utilized to measure target standard article in following 16 kinds of palycyclic aromatics and 3 kinds, its retention time, qualitative, quota ion is as shown in table 1:
Show target mass spectrophotometry parameter in 1:16 kind palycyclic aromatic and 3 kinds
| Compound | Retention time (min) | Qualitative ion | Quota ion |
| D8-naphthalene | 6.74 | 134 | 136 |
| Naphthalene | 6.86 | 129,127 | 128 |
| Acenaphthylene | 11.78 | 151,153 | 152 |
| Acenaphthene | 12.47 | 154,152 | 153 |
| Fluorenes | 14.44 | 165,167 | 166 |
| D10-is luxuriant and rich with fragrance | 18.66 | 184 | 188 |
| Luxuriant and rich with fragrance | 18.71 | 179,176 | 178 |
| Anthracene | 18.93 | 179,176 | 178 |
| Fluoranthene | 24.17 | 200,203 | 202 |
| Pyrene | 25.15 | 200,203 | 202 |
| Benzo [a] anthracene | 30.81 | 229,226 | 228 |
| Bend | 30.87 | 229,226 | 228 |
| Benzo [b] fluoranthene | 35.46 | 253,250 | 252 |
| Benzo [k] fluoranthene | 35.58 | 253,250 | 252 |
| D12-benzo [a] pyrene | 36.50 | 263 | 264 |
| Benzo [a] pyrene | 36.61 | 253,250 | 252 |
| Indenols [1,2,3-cd] pyrene | 41.53 | 277,138 | 276 |
| Dibenzo [a, h] anthracene | 41.78 | 276,139 | 278 |
| Benzo [g, h, i] perylene | 42.77 | 277,138 | 276 |
A certain tobacco juice for electronic smoke sample is added 16 kinds of palycyclic aromatic standard solution of high, medium and low three contents levels, do recovery experiment, the in a few days repeatability of simultaneously carrying out the method measures with repeated in the daytime, and represent by the relative standard deviation (RSD) of measurement result, result is as shown in table 2.Visible, this method repeatability and the recovery very well, are applicable to the analysis of batch samples.
Table 2: the repeatability of method and recovery testing result
| Compound | In RSD(day, %) | Between RSD(day, %) | The recovery (%) |
| Naphthalene | 2.74 | 3.04 | 92.1 |
| Acenaphthylene | 3.21 | 5.03 | 94.2 |
| Acenaphthene | 3.29 | 5.64 | 91.2 |
| Fluorenes | 4.54 | 6.59 | 98.5 |
| Luxuriant and rich with fragrance | 5.21 | 6.29 | 100.2 |
| Anthracene | 6.54 | 7.56 | 100.8 |
| Fluoranthene | 3.71 | 4.94 | 94.5 |
| Pyrene | 3.42 | 5.93 | 92.6 |
| Benzo [a] anthracene | 2.29 | 3.64 | 99.5 |
| Bend | 3.51 | 4.59 | 92.2 |
| Benzo [b] fluoranthene | 2.21 | 3.28 | 98.5 |
| Benzo [k] fluoranthene | 4.54 | 6.52 | 99.2 |
| Benzo [a] pyrene | 2.71 | 4.94 | 99.8 |
| Indenols [1,2,3-cd] pyrene | 3.52 | 5.93 | 94.5 |
| Dibenzo [a, h] anthracene | 2.59 | 3.64 | 92.6 |
| Benzo [g, h, i] perylene | 3.61 | 4.69 | 98.3 |
The present invention, compared with existing detection method, has following technical advantage:
1, solid phase is supported that liquid-liquid technique is applied to the mensuration of 16 kinds of palycyclic aromatics in tobacco juice for electronic smoke by the present invention first, by inertia porous mass-calcined diatomite medium, extraction step only needs a step to complete, for a kind of novel liquid-liquid extracting process, instead of separating funnel, simplify extraction step and emulsion can not occur.Because tobacco juice for electronic smoke principal ingredient is polar solvent, be suitable for solid phase and support liquid-liquid technique, can avoid adopting and measure the conventional solid phase extraction of palycyclic aromatic, eliminate the steps such as loaded down with trivial details activation, drip washing, wash-out, enormously simplify sample pre-treatments, be applicable to the analysis of batch samples, save organic solvent use, more environmental protection simultaneously.
2, this method recovery is between 91.2%-100.8%, and withinday precision is between 2.74%-6.54%, and day to day precision is between 3.04%-7.56%, and the method recovery and reproducible is described, actual application value is high.
Accompanying drawing explanation
Target standard colors spectrogram in Fig. 1: 16 kinds of palycyclic aromatics and 3 kinds,
In figure: 1-D
8-naphthalene; 2-naphthalene; 3-acenaphthylene; 4-acenaphthene, 5-fluorenes, 6-D
10-Fei, 7-luxuriant and rich with fragrance, 8-anthracene, 9-fluoranthene, 10-pyrene, 11-benzo [a] anthracene, 12-bend, 13-benzo [b] fluoranthene, 14-benzo [k] fluoranthene, 15-D
12-benzo [a] pyrene, 16-benzo [a] pyrene, 17-indenols-[1,2,3-cd] pyrene, 18-dibenzo [a, h] anthracene, 19-benzo [g, h, i] perylene.
Fig. 2: the chromatogram of tobacco juice for electronic smoke A,
In figure: 1-D
8-naphthalene; 2-naphthalene; 3-D
10-Fei, 4-luxuriant and rich with fragrance, 5-fluoranthene, 6-pyrene.
Embodiment
Below by embodiment, the present invention is further described:
Embodiment 1
1, the extraction of testing sample and concentrated: accurately take about 0.1g electronic cigarette liquid in conical flask, add D
8-Nai, D
10-Fei, D
12mark and 0.9mL water in-benzo [a] pyrene, all proceed to solid phase and support that, in liquid-liquid extraction post, pressurization makes testing sample solution fully spread in column packing after vibration 2min, leave standstill 5min, add 5mL cyclohexane wash-out, collect eluent, be concentrated into about 0.2mL, detect with GC-MS;
2, GC-MS analysis condition: injector temperature: 280 DEG C; Sample size: 1 μ L; Splitless injecting-Sample; Carrier gas: helium, constant current flow velocity 1mL/min; Chromatographic column: DB-5MS, specification 30m × 0.25mm × 0.25 μm; Temperature programme: 60 DEG C keep 1min, then rise to 110 DEG C with the speed of 20 DEG C/min, then rise to 280 DEG C with the speed of 5 DEG C/min, keep 10min.Ionization mode: EI; Transmission line temperature: 280 DEG C; Ion source temperature: 230 DEG C.Scan mode: SIM, Selective ion mode, see table 1, is 50ms to the monitoring time of each ion.
3, the preparation method of hybrid standard working solution is: the 1) preparation of hybrid standard stock solution: accurately take 10mg16 kind palycyclic aromatic respectively in the brown volumetric flask of 100mL, dissolve constant volume with cyclohexane.The preparation of mark stock solution in mixing: accurately how take 10mgD8-respectively, D10-is luxuriant and rich with fragrance, and D12-benzo [a] pyrene, in the brown volumetric flask of 100mL, dissolves constant volume with cyclohexane.2) preparation of hybrid standard working solution: accurately add 5uL respectively, marks stock solution in the brown volumetric flask of 50mL in 10uL, 20uL, 40uL, 80uL hybrid standard stock solution and 40uL mixing, uses cyclohexane constant volume.
4, Specification Curve of Increasing and result calculate: carry out area integral to the response of series standard solution, do calibration curve, carry out matching, obtain unary linear regression equation, coefficient R with straight line with the ratio of often kind of palycyclic aromatic and interior target response area comparison content
2should 0.999 be not less than.The sample of preparation is measured, records often kind of palycyclic aromatic in sample and interior mark peak area ratio, bring unary linear regression equation into, calculate often kind of polycyclic aromatic hydrocarbon content.
According to the method for the assay method of 16 kinds of palycyclic aromatics in foregoing a kind of tobacco juice for electronic smoke, select a kind of tobacco juice for electronic smoke A, record wherein how, the content of phenanthrene, fluoranthene, pyrene is respectively 35.35ng/g, 11.87ng/g, 3.28ng/g, 2.53ng/g, as shown in Figure 2, other palycyclic aromatics do not detect.
Embodiment 2
1, the extraction of testing sample and concentrated: accurately take about 3g electronic cigarette liquid in conical flask, add D
8-Nai, D
10-Fei, D
12mark and 2mL water in-benzo [a] pyrene, all proceed to solid phase and support that, in liquid-liquid extraction post, pressurization makes testing sample solution fully spread in column packing after vibration 5min, leave standstill 5min, add 24mL cyclohexane wash-out, collect eluent, be concentrated into about 1mL, detect with GC-MS;
2, GC-MS analysis condition: injector temperature: 280 DEG C; Sample size: 1 μ L; Splitless injecting-Sample; Carrier gas: helium, constant current flow velocity 1mL/min; Chromatographic column: DB-5MS, specification 30m × 0.25mm × 0.25 μm; Temperature programme: 60 DEG C keep 1min, then rise to 110 DEG C with the speed of 20 DEG C/min, then rise to 280 DEG C with the speed of 5 DEG C/min, keep 10min.Ionization mode: EI; Transmission line temperature: 280 DEG C; Ion source temperature: 230 DEG C.Scan mode: SIM, Selective ion mode, see table 1, is 50ms to the monitoring time of each ion.
3, the preparation method of hybrid standard working solution is: the 1) preparation of hybrid standard stock solution: accurately take 10mg16 kind palycyclic aromatic respectively in the brown volumetric flask of 100mL, dissolve constant volume with cyclohexane.The preparation of mark stock solution in mixing: accurately how take 10mgD8-respectively, D10-is luxuriant and rich with fragrance, and D12-benzo [a] pyrene, in the brown volumetric flask of 100mL, dissolves constant volume with cyclohexane.2) preparation of hybrid standard working solution: accurately add 5uL respectively, marks stock solution in the brown volumetric flask of 50mL in 10uL, 20uL, 40uL, 80uL hybrid standard stock solution and 40uL mixing, uses cyclohexane constant volume.
4, Specification Curve of Increasing and result calculate: carry out area integral to the response of series standard solution, do calibration curve, carry out matching, obtain unary linear regression equation, coefficient R with straight line with the ratio of often kind of palycyclic aromatic and interior target response area comparison content
2should 0.999 be not less than.The sample of preparation is measured, records often kind of palycyclic aromatic in sample and interior mark peak area ratio, bring unary linear regression equation into, calculate often kind of polycyclic aromatic hydrocarbon content.
According to foregoing assay method, select a kind of tobacco juice for electronic smoke B, how record wherein, the content of phenanthrene, anthracene, fluoranthene, pyrene is respectively 38.06ng/g, 7.97ng/g, 2.32ng/g, 4.33ng/g, 2.80ng/g, other palycyclic aromatics do not detect.
Claims (5)
1. the assay method of 16 kinds of palycyclic aromatics in a tobacco juice for electronic smoke, it is characterized in that: the method adds a small amount of water by tobacco juice for electronic smoke sample, shaken well, being well dispersed in zeyssatite solid phase supports in liquid-liquid extraction post, extract with cyclohexane, after extract is concentrated, gas chromatograph-mass spectrometer (GCMS) (GC-MS) is adopted to realize the mensuration of 16 kinds of palycyclic aromatics in tobacco juice for electronic smoke, described 16 kinds of palycyclic aromatics are: naphthalene, acenaphthylene, acenaphthene, fluorenes, luxuriant and rich with fragrance, anthracene, fluoranthene, pyrene, benzo [a] anthracene, bend, benzo [b] fluoranthene, benzo [k] fluoranthene, benzo [a] pyrene, indenols-[1, 2, 3-cd] pyrene, dibenzo [a, h] anthracene, benzo [g, h, i] perylene, concrete determination step is as follows:
(1) extraction of testing sample and concentrated: accurately take 0.1 ~ 3g electronic cigarette liquid in conical flask, add D
8-naphthalene, D
10-Fei, D
12mark and 0.9 ~ 3mL water in-benzo [a] pyrene 3 kinds, all proceeding to solid phase after vibration 2 ~ 10min supports in liquid-liquid extraction post, pressurization makes testing sample solution fully spread in column packing, leave standstill 2 ~ 10min, add 5 ~ 24mL cyclohexane wash-out, collect eluent, be concentrated into 0.2 ~ 1mL, detect with GC-MS;
(2) preparation of standard working solution: preparation to have in 16 kinds of palycyclic aromatics of concentration gradient and 3 kinds target cyclohexane solution as hybrid standard working solution;
(3) GC-MS of sample analyzes,
(4) Specification Curve of Increasing and result calculate.
2. the assay method of 16 kinds of palycyclic aromatics in tobacco juice for electronic smoke according to claim 1, is characterized in that: GC-MS analysis condition:
Injector temperature: 280 DEG C; Sample size: 1 μ L; Splitless injecting-Sample; Carrier gas: helium, constant current flow velocity 1mL/min; Chromatographic column: DB-5MS, specification 30m × 0.25mm × 0.25 μm; Temperature programme: 60 DEG C keep 1min, then rise to 110 DEG C with the speed of 20 DEG C/min, then rise to 280 DEG C with the speed of 5 DEG C/min, keep 10min;
Ionization mode: EI; Transmission line temperature: 280 DEG C; Ion source temperature: 230 DEG C;
Scan mode: SIM, Selective ion mode, see table 1, is 50ms to the monitoring time of each ion;
Show target mass spectrophotometry parameter in 1:16 kind palycyclic aromatic and 3 kinds
。
3. the assay method of 16 kinds of palycyclic aromatics in tobacco juice for electronic smoke according to claim 1, is characterized in that: the preparation method of hybrid standard working solution is as follows:
1) preparation of hybrid standard stock solution: accurately take 10mg16 kind palycyclic aromatic respectively in the brown volumetric flask of 100mL, dissolve constant volume with cyclohexane; The preparation of mark stock solution in mixing: accurately take 10mgD respectively
8-naphthalene, D
10-Fei, D
12-benzo [a] pyrene, in the brown volumetric flask of 100mL, dissolves constant volume with cyclohexane;
2) preparation of hybrid standard working solution: accurately add 5 μ L respectively, 10 μ L, 20 μ L, 40 μ L, in 80 μ L hybrid standard stock solutions and 40 μ L mix, mark stock solution is in 50mL volumetric flask, uses cyclohexane constant volume.
4. the assay method of 16 kinds of palycyclic aromatics in tobacco juice for electronic smoke according to claim 1, it is characterized in that: Specification Curve of Increasing and result calculate specific as follows: carry out area integral to the response of series standard solution, calibration curve is done with the ratio of often kind of palycyclic aromatic and interior target response area comparison content, carry out matching with straight line, obtain unary linear regression equation; The sample of preparation is measured, records often kind of palycyclic aromatic in sample and interior mark peak area ratio, bring unary linear regression equation into, calculate often kind of polycyclic aromatic hydrocarbon content.
5. the assay method of 16 kinds of palycyclic aromatics in tobacco juice for electronic smoke according to claim 1, is characterized in that: when sample solution wash-out, and controlling the eluent rate of outflow is 2mL/min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510233951.8A CN104764851B (en) | 2015-05-11 | 2015-05-11 | The assay method of 16 kinds of palycyclic aromatics in a kind of tobacco juice for electronic smoke |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510233951.8A CN104764851B (en) | 2015-05-11 | 2015-05-11 | The assay method of 16 kinds of palycyclic aromatics in a kind of tobacco juice for electronic smoke |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104764851A CN104764851A (en) | 2015-07-08 |
| CN104764851B true CN104764851B (en) | 2016-02-24 |
Family
ID=53646812
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510233951.8A Active CN104764851B (en) | 2015-05-11 | 2015-05-11 | The assay method of 16 kinds of palycyclic aromatics in a kind of tobacco juice for electronic smoke |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104764851B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105181857A (en) * | 2015-09-07 | 2015-12-23 | 国家烟草质量监督检验中心 | Method for measuring polycyclic aromatic hydrocarbons in cigarette cut tobacco by on-line solid phase extraction and high performance liquid chromatography |
| CN105136931A (en) * | 2015-09-07 | 2015-12-09 | 国家烟草质量监督检验中心 | Method for determining polyaromatic hydrocarbons in smokeless tobacco product by utilization of on-line solid phase extraction high performance liquid chromatography |
| CN106226417B (en) * | 2016-07-12 | 2018-12-25 | 吉林烟草工业有限责任公司 | The detection method of polyol ethers and its ester type compound in a kind of tobacco juice for electronic smoke |
| CN106645444B (en) * | 2016-09-28 | 2019-06-04 | 云南省烟草质量监督检测站 | A kind of method of multiring aromatic hydrocarbon substance content in detection smoke-free tobacco product |
| CN106248835B (en) * | 2016-09-28 | 2019-05-31 | 云南省烟草质量监督检测站 | The method for detecting 22 kinds of multiring aromatic hydrocarbon substance contents in cigarette smoke simultaneously |
| CN108627591B (en) * | 2017-03-24 | 2021-03-02 | 上海安谱实验科技股份有限公司 | Method for extracting polycyclic aromatic hydrocarbon from PA6 product |
| CN109828050A (en) * | 2019-03-08 | 2019-05-31 | 中钢集团鞍山热能研究院有限公司 | A kind of method of 3,4- BaP content in quick measurement coal tar, coal tar pitch |
| CN110927264A (en) * | 2019-11-08 | 2020-03-27 | 河北省医疗器械与药品包装材料检验研究院(河北省医疗器械技术审评中心) | Method and kit for detecting glucocorticoid in serum |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6190922B1 (en) * | 1998-04-16 | 2001-02-20 | Strategic Diagnostics Inc. | Substrate supported liquid extraction |
| CN102879484B (en) * | 2011-07-14 | 2014-05-21 | 中国烟草总公司郑州烟草研究院 | Method for determining content of benzo[a]pyrene in cigarette mainstream smoke |
| CN103323543B (en) * | 2013-05-21 | 2015-06-17 | 上海烟草集团有限责任公司 | Method for detecting 17 polycyclic aromatic hydrocarbons in cigarette gas |
| ES2720883T3 (en) * | 2013-08-21 | 2019-07-25 | Biotage Ab | Sample preparation workstation |
| CN103439443B (en) * | 2013-08-29 | 2015-08-26 | 中国烟草总公司郑州烟草研究院 | The method of 16 kinds of palycyclic aromatics in Simultaneously test cigarette mainstream flue gas |
| CN203502394U (en) * | 2013-09-23 | 2014-03-26 | 江苏中烟工业有限责任公司 | Device for extracting and purifying polyaromatic hydrocarbon in cigarette smoke |
-
2015
- 2015-05-11 CN CN201510233951.8A patent/CN104764851B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104764851A (en) | 2015-07-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104764851B (en) | The assay method of 16 kinds of palycyclic aromatics in a kind of tobacco juice for electronic smoke | |
| CN101701949B (en) | Measurement method of volatile organic compound in tobacco lining paper | |
| CN104655766B (en) | Assay method while nicotine, propylene glycol and glycerol in a kind of tobacco juice for electronic smoke | |
| CN105866267B (en) | The extraction of residual solvent, detection method and kit and purposes in packaging material | |
| CN106290595B (en) | On-line monitoring method for volatile organic compounds in atmosphere | |
| CN102608244A (en) | Detection method for simultaneously determining plurality of flavor substances in cigarette cut tobaccos | |
| CN103323543B (en) | Method for detecting 17 polycyclic aromatic hydrocarbons in cigarette gas | |
| CN104020246A (en) | Method for simultaneously detecting plurality of volatile trace carbonyl compounds in atmosphere | |
| CN103675134B (en) | Method for detecting benzene series pollutants in gas by using internal standard method | |
| CN106841442B (en) | Method for simultaneously determining vinyl acetate and six benzene series in water-based adhesive | |
| CN106645444B (en) | A kind of method of multiring aromatic hydrocarbon substance content in detection smoke-free tobacco product | |
| CN105067728A (en) | Method for measuring solvent compositions in nicotine liquid by combining gas chromatography and mass spectrometry | |
| CN104655767A (en) | Method for simultaneously determining vanillin, ethyl vanillin and coumarin in electronic cigarette solution | |
| CN106645443A (en) | Method for detecting short-chain chlorinated paraffin (SCCP) and medium-chain chlorinated paraffin (MCCP) in consumer goods | |
| CN105424845A (en) | Method for analyzing aroma components of flavoring material through GC/MS based on thermal desorption | |
| Escuderos | Olive oil aroma evaluation by gas chromatographic method: a critical review | |
| CN104655753A (en) | Method for determining 3-acetyl-2, 5-thioxene in food additive | |
| CN104655775A (en) | Method for measuring 3-acetyl-2, 5-dimethyl thiophene in edible flavor and fragrance | |
| CN101539549A (en) | Photoionization gas chromatography detection method of organic volatile compounds in air | |
| CN103743844A (en) | Determination method of alcohol content in methanol | |
| CN106198796B (en) | A kind of method of benzo [a] pyrene in supercritical fluid chromatography-Gas Chromatography-Mass Spectrometry cigarette mainstream flue gas | |
| CN104198605B (en) | A kind of assay method of cigarette hot melt adhesive phenol antioxidant content | |
| CN106248835B (en) | The method for detecting 22 kinds of multiring aromatic hydrocarbon substance contents in cigarette smoke simultaneously | |
| CN105181868A (en) | Method for simultaneous detection of 13 benzene compounds and phthalic acid ester compounds in water-borne adhesive and application thereof | |
| CN104280474B (en) | High performance liquid chromatography fluorescence detection method for simultaneously detecting 4 forbidden additives in tobacco flavor and fragrance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |