CN104758315A - Method for wrapping fish oil with zein - Google Patents
Method for wrapping fish oil with zein Download PDFInfo
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- CN104758315A CN104758315A CN201410005004.9A CN201410005004A CN104758315A CN 104758315 A CN104758315 A CN 104758315A CN 201410005004 A CN201410005004 A CN 201410005004A CN 104758315 A CN104758315 A CN 104758315A
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Abstract
The invention provides a method for wrapping fish oil with zein. The method comprises the following steps: S1, dissolving zein in ethanol water, and stirring till the ethanol water is homogeneous and transparent to prepare a zein solution; S2, dissolving fish oil in ethanol water, and stirring till the ethanol water is homogeneous and transparent to prepare a fish oil solution; S3, mixing the zein solution and the fish oil solution; and S4, drying the mixture. According to the method, fish oil is wrapped through evaporation self-assembly of zein to be better protected, reduction of both nutrition and medicinal value of fish oil is avoided, the flavor of fish oil is eliminated, and the solubility property of fish oil is changed. Meanwhile, fish oil can be wrapped without complicated equipment, so that the preparation method is simple. The obtained fish oil can be widely applied to the fields of food, medicines and the like.
Description
Technical field
The invention belongs to both sexes natural macromolecular material technical field, in particular, relate to a kind of method utilizing zein to wrap up fish oil.
Background technology
Be rich in abundant eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in fish oil, these omega-3 unsaturated fatty acids are materials with different physiological roles important in fish oil, have higher nutritive value even medical value.Research shows, EPA and DHA safeguards there is active influence to the brain development of teenager and adult and function, can also be used to treat many cardiovascular disease, in the treatment and prevention of cancer, also played important effect.But due in these polyunsaturated fatty acid containing more double bond, be easy to oxidized rotten under the condition of illumination and aerobic, become sour, cause the disappearance of nutritive value and medical value; In addition, the taste of fish oil can give people a kind of offending sensation.For these two kinds of reasons, many different measures have been used to the protection of fish oil, comprising the micro encapsulation of fish oil.In microencapsulation technology, the core material of different conditions is wrapped up by natural or synthesized polymer material, and can be released under certain condition.Research shows, fish oil can by the protection of many different materials parcels.These lappings can be that single also can being combined by different materials is formed, and comprise lactalbumin, defatted milk powder, casein, upgraded fiber element, gelatin-Arabic gum coacervate and chitosan.But successfully in these parcel examples, used lapping is all comparatively complicated, and the step of packaging method is also comparatively various.
Zein is the major storage albumen in Semen Maydis, is the by-product of corn wet processing.Because it contains the hydrophobic group of more than 50%, zein is a kind of Amphiphilic proteins.This comparatively cheap albumen serves many purposes, and such as, with zein parcel oleum lini and quintessence oil, zein is combined with other materials such as chitosan by other research wraps up vitamin e.But what not yet have research institution to be applied to fish oil to zein at present was enclosed with report.
Summary of the invention
Technical problem to be solved by this invention is: provide a kind of method utilizing zein to wrap up fish oil, solve existing fish oil packaging method complicated, the problems such as lapping is not single, and preparation cost is high.
Wrap up in the method for fish oil at the zein that utilizes of the present invention, comprise the steps:
S1, zein is dissolved in ethanol water, is stirred to dissolution homogeneity transparent, thus makes zein solution;
S2, fish oil is dissolved in ethanol water, is stirred to dissolution homogeneity transparent, thus makes fish oil solution;
S3, described zein solution to be mixed with described fish oil solution;
S4, drying is carried out to the mixed solution of described zein solution and described fish oil solution, obtain the zein-fish oil powder forming nucleocapsid structure with zein with microsphere shape parcel fish oil.
Wrap up in the method for fish oil at the zein that utilizes of the present invention, in the mixed solution of described zein solution and described fish oil solution, the mass ratio of described fish oil and described zein is 3 ~ 10 ﹕ 10.
Wrap up in the method for fish oil at the zein that utilizes of the present invention, the stirring means used in described step S1 and described step S2 is ultrasonic agitation.
Of the present invention utilize zein wrap up fish oil method in, the ethanol water used in described step S1 for volume fraction be the ethanol water of 60% ~ 90%.
Of the present invention utilize zein wrap up fish oil method in, the ethanol water used in described step S2 for volume fraction be the ethanol water of 60% ~ 90%.
Wrap up in the method for fish oil at the zein that utilizes of the present invention, in described zein solution, the content of zein is 0.3mg/ml ~ 1.0mg/ml.
Wrap up in the method for fish oil at the zein that utilizes of the present invention, in described fish oil solution, the content of fish oil is 0.3mg/ml ~ 1.0mg/ml.
Wrap up in the method for fish oil at the zein that utilizes of the present invention, the drying means used in described step S4 is natural drying.
Wrap up in the method for fish oil at the zein that utilizes of the present invention, described step S4 comprises further: by the described zein of dry gained-fish oil powder petroleum ether cleaning at least one times.
The invention has the beneficial effects as follows; by wrapping up fish oil with zein evaporation self assembly; better can protect fish oil; avoid the reduction of fish oil nutrition and medical value, eliminate the taste of fish oil, change the dissolution properties of fish oil; simultaneously; to in the encapsulation process of fish oil without the need to using complex device, preparation method is simple, and the product obtained can be widely used in food, medicine and other fields.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme in the embodiment of the present invention, below the accompanying drawing used required in describing embodiment is briefly described, apparently, accompanying drawing in the following describes is only one embodiment of the present of invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the FTIR spectrum figure of pure fish oil and pure zein;
Fig. 2 is the mass ratio of fish oil and zein is 4 ﹕ 10, and concentration of alcohol is 80%(v/v) time gained zein-fish oil powder FTIR spectrum figure;
Fig. 3 is the mass ratio of fish oil and zein is 3 ﹕ 10, and concentration of alcohol is 80%(v/v) time gained zein-fish oil powder FTIR spectrum figure;
Fig. 4 is the mass ratio of fish oil and zein is 5 ﹕ 10, and concentration of alcohol is 80%(v/v) time gained zein-fish oil powder FTIR spectrum figure;
Fig. 5 is the mass ratio of fish oil and zein is 6 ﹕ 10, and concentration of alcohol is 80%(v/v) time gained zein-fish oil powder FTIR spectrum figure;
Fig. 6 is the mass ratio of fish oil and zein is 8 ﹕ 10, and concentration of alcohol is 80%(v/v) time gained zein-fish oil powder FTIR spectrum figure;
Fig. 7 is the mass ratio of fish oil and zein is 10 ﹕ 10, and concentration of alcohol is 80%(v/v) time gained zein-fish oil powder FTIR spectrum figure;
Fig. 8 is the mass ratio of fish oil and zein is 3 ﹕ 10, concentration of alcohol is 70%(v/v) time gained zein-fish oil powder FTIR spectrum figure;
Fig. 9 is the mass ratio of fish oil and zein is 3 ﹕ 10, concentration of alcohol is 60%(v/v) time gained zein-fish oil powder FTIR spectrum figure.
Detailed description of the invention
Below in conjunction with accompanying drawing, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
The invention provides a kind of method utilizing zein to wrap up fish oil, the zein used is natural agricultural product by-product, with low cost, wide material sources, biodegradable and compatible, become a kind of excellent encapsulation material, encapsulation process comprises the steps:
The preparation of S1, zein solution: zein is dissolved in ethanol water, is stirred to dissolution homogeneity transparent;
The preparation of S2, fish oil solution: fish oil is dissolved in ethanol water, is stirred to dissolution homogeneity transparent;
S3, described zein solution to be mixed with described fish oil solution;
S4, drying.
Can there is self assembly along with the evaporation of ethanol and the change of solution polarity in zein, realize the parcel to fish oil in volatile spirit solvent.
Fig. 1 shows fourier conversion infrared spectrogram (FTIR) of pure fish oil and pure zein, wherein two characteristic peak 3010cm
-1(representing the stretching vibrations of cis carbon-carbon double bond) and 1739cm
-1occur in (representing the stretching vibrations of C=O bond) spectrum only not at zein in the spectrum of fish oil, if having any one characteristic peak to occur in the product of preparation, prove that fish oil is wrapped up successfully by zein.In spectral mixture, characteristic peak peak value comparatively may have minute movement in pure substance spectra.Characteristic peak peak value height is relevant with the content of wrapped fish oil.
The present invention also does transmission electron microscope (TEM) structural characterization of product to gained zein-fish oil powder, in the middle of dark zein nucleocapsid spheroid, light spheroid is fish oil drop, confirms that fish oil is rolled into nucleocapsid structure by zein with microsphere shape.
Preferably, in the mixed solution of described zein solution and described fish oil solution, the mass ratio of described fish oil and described zein is 3 ~ 10 ﹕ 10.
Preferably, S1 step and the stirring described in S2 step are ultrasonic agitation, and ultrasonic agitation can better realize dissolving, and zein and fish oil can be well dispersed in ethanol water.
Preferably, the ethanol water used in S1 step and S2 step for volume fraction be the ethanol water of 60% ~ 90%.
Preferably, in described zein solution, the content of zein is 0.3mg/ml ~ 1.0mg/ml.
Preferably, described in described fish oil solution, the content of fish oil is 0.3mg/ml ~ 1.0mg/ml.
Preferably, the drying used in S4 of the present invention is natural drying, the container of wide-mouth is placed in by zein solution obtained above and fish oil solution mixed solution, give free rein to drying at normal temperatures and pressures, realizing from volatilization effect by ethanol in mixed solution, preferred, masking foil should be completed in advance in the large container inside of use, be that products therefrom avoids being attached on container inner wall, be convenient to the collection of product.
Preferably, described S4 step comprises further: dry gained zein-fish oil powder petroleum ether cleaning at least one times, to go out not by zein parcel, the fish oil being attached to powder surface, only need petroleum ether to flow through powder surface slowly during cleaning, the petroleum ether of various boiling range all may be used for above-mentioned cleaning process.
Embodiment 1
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 12mg simultaneously, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.4mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 4 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.The TEM image of gained zein-fish oil powder confirms that fish oil is rolled into nucleocapsid structure by zein with microsphere shape.Fig. 2 shows the FTIR spectrum figure of gained zein-fish oil powder, and gained zein-fish oil powder petroleum ether cleaning secondary is to remove the not wrapped fish oil of powder surface, and FTIR spectrum figure confirms have fish oil to be wrapped up by zein.
Embodiment 2
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 9mg simultaneously, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.3mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 3 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Fig. 3 shows the FTIR spectrum figure of gained zein-fish oil powder, and gained zein-fish oil powder petroleum ether cleaning is once to remove the not wrapped fish oil of powder surface, and FTIR spectrum figure confirms have fish oil to be wrapped up by zein.
Embodiment 3
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 15mg simultaneously, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.5mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 5 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Fig. 4 shows the FTIR spectrum figure of gained zein-fish oil powder, and gained zein-fish oil powder petroleum ether cleaning secondary is to remove the not wrapped fish oil of powder surface, and FTIR spectrum figure confirms have fish oil to be wrapped up by zein.
Embodiment 4
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 18mg simultaneously, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.6mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 6 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Fig. 5 shows the FTIR spectrum figure of gained zein-fish oil powder, and gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, and FTIR spectrum figure confirms have fish oil to be wrapped up by zein.
Embodiment 5
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 24mg simultaneously, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.8mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 8 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Fig. 6 shows the FTIR spectrum figure of gained zein-fish oil powder, and gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, and FTIR spectrum figure confirms have fish oil to be wrapped up by zein.
Embodiment 6
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 30mg simultaneously, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 10 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Fig. 7 shows the FTIR spectrum figure of gained zein-fish oil powder, and gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, and FTIR spectrum figure confirms have fish oil to be wrapped up by zein.
Embodiment 7
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 70%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 9mg simultaneously, be dissolved in the 70%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.3mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 3 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Fig. 8 shows the FTIR spectrum figure of gained zein-fish oil powder, and gained zein-fish oil powder petroleum ether cleaning is once to remove the not wrapped fish oil of powder surface, and FTIR spectrum figure confirms have fish oil to be wrapped up by zein.
Embodiment 8
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 60%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 9mg simultaneously, be dissolved in the 60%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.3mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 3 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Fig. 9 shows the FTIR spectrum figure of gained zein-fish oil powder, and gained zein-fish oil powder petroleum ether cleaning is once to remove the not wrapped fish oil of powder surface, and FTIR spectrum figure confirms have fish oil to be wrapped up by zein.
Embodiment 9
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 90%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 9mg simultaneously, be dissolved in the 90%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.3mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 3 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Gained zein-fish oil powder petroleum ether cleaning is once to remove the not wrapped fish oil of powder surface, the FTIR spectrum figure of gained zein-fish oil powder is similar to Example 8, hereby omit, fish oil also can be wrapped up by zein.
Embodiment 10
Under normal temperature and pressure, take the zein of 9mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 0.3mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 9mg simultaneously, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.3mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 10 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, the FTIR spectrum figure of gained zein-fish oil powder is similar to Example 6, hereby omit, fish oil also can be wrapped up by zein.
Embodiment 11
Under normal temperature and pressure, take the zein of 12mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 0.4mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 12mg simultaneously, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.4mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 10 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, the FTIR spectrum figure of gained zein-fish oil powder is similar to Example 6, hereby omit, fish oil also can be wrapped up by zein.
Embodiment 12
Under normal temperature and pressure, take the zein of 15mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 0.5mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 15mg simultaneously, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.5mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 10 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, the FTIR spectrum figure of gained zein-fish oil powder is similar to Example 6, hereby omit, fish oil also can be wrapped up by zein.
Embodiment 13
Under normal temperature and pressure, take the zein of 18mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 0.6mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 18mg simultaneously, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.6mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 10 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, the FTIR spectrum figure of gained zein-fish oil powder is similar to Example 6, hereby omit, fish oil also can be wrapped up by zein.
Embodiment 14
Under normal temperature and pressure, take the zein of 24mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 0.8mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 24mg simultaneously, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the fish oil solution of 0.8mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 10 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, the FTIR spectrum figure of gained zein-fish oil powder is similar to Example 6, hereby omit, fish oil also can be wrapped up by zein.
Embodiment 15
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 9mg simultaneously, be dissolved in the 80%(v/v of 9ml) ethanol-water solution in, be mixed with the fish oil solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 3 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, the FTIR spectrum figure of gained zein-fish oil powder is similar to Example 2, hereby omit, fish oil also can be wrapped up by zein.
Embodiment 16
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 12mg simultaneously, be dissolved in the 80%(v/v of 12ml) ethanol-water solution in, be mixed with the fish oil solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 4 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, the FTIR spectrum figure of gained zein-fish oil powder is similar to Example 1, hereby omit, fish oil also can be wrapped up by zein.
Embodiment 17
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 15mg simultaneously, be dissolved in the 80%(v/v of 15ml) ethanol-water solution in, be mixed with the fish oil solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 5 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, the FTIR spectrum figure of gained zein-fish oil powder is similar to Example 3, hereby omit, fish oil also can be wrapped up by zein.
Embodiment 18
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 18mg simultaneously, be dissolved in the 80%(v/v of 18ml) ethanol-water solution in, be mixed with the fish oil solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 6 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, the FTIR spectrum figure of gained zein-fish oil powder is similar to Example 4, hereby omit, fish oil also can be wrapped up by zein.
Embodiment 19
Under normal temperature and pressure, take the zein of 30mg, be dissolved in the 80%(v/v of 30ml) ethanol-water solution in, be mixed with the zein solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity; Take the fish oil of 24mg simultaneously, be dissolved in the 80%(v/v of 24ml) ethanol-water solution in, be mixed with the fish oil solution of 1mg/ml, the about 10min of ultrasonic agitation, transparent to dissolution homogeneity.Immediately gained zein solution is mixed with gained fish oil solution afterwards, now in gained mixed solution, the mass ratio of fish oil and zein is 8 ﹕ 10, and gained mixed solution is introduced into preprepared and completes natural drying in the culture dish of masking foil.Gained zein-fish oil powder petroleum ether cleans three times to remove the not wrapped fish oil of powder surface, the FTIR spectrum figure of gained zein-fish oil powder is similar to Example 5, hereby omit, fish oil also can be wrapped up by zein.
Above the method utilizing zein to wrap up fish oil provided by the present invention is described in detail.It is to be noted; content described by detailed description of the invention is for better implementing the present invention and preferred embodiment; protection scope of the present invention is not limited to the technical scheme described in above-mentioned embodiment; and should be as the criterion with the flesh and blood described in claims, any possible composition, content and technologic change are only otherwise the flesh and blood departing from the claims in the present invention all belongs to the scope that the present invention protects.
Claims (9)
1. utilize zein to wrap up a method for fish oil, it is characterized in that, comprise the steps:
S1, zein is dissolved in ethanol water, is stirred to dissolution homogeneity transparent, thus makes zein solution;
S2, fish oil is dissolved in ethanol water, is stirred to dissolution homogeneity transparent, thus makes fish oil solution;
S3, described zein solution to be mixed with described fish oil solution;
S4, drying is carried out to the mixed solution of described zein solution and described fish oil solution, obtain the zein-fish oil powder forming nucleocapsid structure with zein with microsphere shape parcel fish oil.
2. according to the method described in claim 1, it is characterized in that, in the mixed solution of described zein solution and described fish oil solution, the mass ratio of described fish oil and described zein is 3 ~ 10 ﹕ 10.
3. according to the method described in claim 2, it is characterized in that, the stirring means used in described step S1 and described step S2 is ultrasonic agitation.
4., according to the method described in claim 2, it is characterized in that, the ethanol water used in described step S1 for volume fraction be the ethanol water of 60% ~ 90%.
5., according to the method described in claim 2, it is characterized in that, the ethanol water used in described step S2 for volume fraction be the ethanol water of 60% ~ 90%.
6. according to the method described in claim 2, it is characterized in that, in described zein solution, the content of zein is 0.3mg/ml ~ 1.0mg/ml.
7. according to the method described in claim 2, it is characterized in that, in described fish oil solution, the content of fish oil is 0.3mg/ml ~ 1.0mg/ml.
8. according to the method described in claim 2, it is characterized in that, the drying means used in described step S4 is natural drying.
9. according to the method described in claim 2, it is characterized in that, described step S4 comprises further: by the described zein of dry gained-fish oil powder petroleum ether cleaning at least one times.
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| CN201410005004.9A CN104758315B (en) | 2014-01-06 | 2014-01-06 | A method of fish oil is wrapped up using zeins |
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Cited By (13)
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| CN105558354A (en) * | 2016-01-26 | 2016-05-11 | 武汉轻工大学 | Feed-use fish oil microcapsule and preparation process thereof |
| CN106723053A (en) * | 2016-12-30 | 2017-05-31 | 北京和益源生物技术有限公司 | A kind of water stabilization DHA microcapsules and its preparation method and application |
| CN107595809A (en) * | 2017-09-19 | 2018-01-19 | 云智前沿科技发展（深圳）有限公司 | A kind of zeins nanometer embedding sustained release filler and preparation method thereof |
| CN107821643A (en) * | 2017-10-23 | 2018-03-23 | 中国科学院兰州化学物理研究所 | A kind of preparation method of matrimony vine powdered oil |
| CN108403659A (en) * | 2018-03-01 | 2018-08-17 | 中国科学院过程工程研究所 | A kind of hard emulsion receives microballoon and its preparation method and application |
| CN109170284A (en) * | 2018-10-27 | 2019-01-11 | 上海衡威生物技术安徽有限公司 | It is a kind of for improving the protective agent of weak pigle growth performance |
| CN109714985A (en) * | 2016-09-26 | 2019-05-03 | 株式会社山田养蜂场本社 | The enzyme that stable and taste is improved decomposes royal jelly particle |
| CN110314220A (en) * | 2018-03-28 | 2019-10-11 | 巨野恒丰果蔬有限公司 | A kind of garlic albumen and garlic oil compounding agent and the preparation method and application thereof |
| CN111905106A (en) * | 2020-09-14 | 2020-11-10 | 陕西科技大学 | Slow-release sodium alginate/chitosan/zein composite material and preparation method thereof |
| CN112402291A (en) * | 2020-11-26 | 2021-02-26 | 上海应用技术大学 | Composite wall material particle coated with fat-soluble sunscreen agent, preparation method and application |
| CN112675312A (en) * | 2020-12-30 | 2021-04-20 | 香港理工大学深圳研究院 | Brain-targeting nano delivery system and preparation method thereof |
| CN113576003A (en) * | 2020-04-30 | 2021-11-02 | 内蒙古蒙牛乳业（集团）股份有限公司 | Microcapsules containing active substances and method for the production thereof |
| CN114592283A (en) * | 2022-02-21 | 2022-06-07 | 江苏大学 | Preparation method of flavor substance nanofiber membrane based on controlled release embedding |
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| CN105558354A (en) * | 2016-01-26 | 2016-05-11 | 武汉轻工大学 | Feed-use fish oil microcapsule and preparation process thereof |
| CN105558354B (en) * | 2016-01-26 | 2019-08-20 | 武汉轻工大学 | Feeding fish oil micro-capsule and its preparation process |
| CN109714985A (en) * | 2016-09-26 | 2019-05-03 | 株式会社山田养蜂场本社 | The enzyme that stable and taste is improved decomposes royal jelly particle |
| CN106723053A (en) * | 2016-12-30 | 2017-05-31 | 北京和益源生物技术有限公司 | A kind of water stabilization DHA microcapsules and its preparation method and application |
| CN107595809B (en) * | 2017-09-19 | 2020-06-19 | 云智前沿科技发展（深圳）有限公司 | Zein nano embedded slow-release filler and preparation method thereof |
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| CN107821643A (en) * | 2017-10-23 | 2018-03-23 | 中国科学院兰州化学物理研究所 | A kind of preparation method of matrimony vine powdered oil |
| CN108403659A (en) * | 2018-03-01 | 2018-08-17 | 中国科学院过程工程研究所 | A kind of hard emulsion receives microballoon and its preparation method and application |
| CN110314220A (en) * | 2018-03-28 | 2019-10-11 | 巨野恒丰果蔬有限公司 | A kind of garlic albumen and garlic oil compounding agent and the preparation method and application thereof |
| CN109170284A (en) * | 2018-10-27 | 2019-01-11 | 上海衡威生物技术安徽有限公司 | It is a kind of for improving the protective agent of weak pigle growth performance |
| CN113576003A (en) * | 2020-04-30 | 2021-11-02 | 内蒙古蒙牛乳业（集团）股份有限公司 | Microcapsules containing active substances and method for the production thereof |
| CN111905106A (en) * | 2020-09-14 | 2020-11-10 | 陕西科技大学 | Slow-release sodium alginate/chitosan/zein composite material and preparation method thereof |
| CN112402291A (en) * | 2020-11-26 | 2021-02-26 | 上海应用技术大学 | Composite wall material particle coated with fat-soluble sunscreen agent, preparation method and application |
| CN112675312A (en) * | 2020-12-30 | 2021-04-20 | 香港理工大学深圳研究院 | Brain-targeting nano delivery system and preparation method thereof |
| CN114592283A (en) * | 2022-02-21 | 2022-06-07 | 江苏大学 | Preparation method of flavor substance nanofiber membrane based on controlled release embedding |
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