CN104745637A - Method for preparing selenium nano-particles by taking secretion of microbial cells as matrix - Google Patents
Method for preparing selenium nano-particles by taking secretion of microbial cells as matrix Download PDFInfo
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- CN104745637A CN104745637A CN201510089257.3A CN201510089257A CN104745637A CN 104745637 A CN104745637 A CN 104745637A CN 201510089257 A CN201510089257 A CN 201510089257A CN 104745637 A CN104745637 A CN 104745637A
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Abstract
The invention discloses a method for preparing selenium nano-particles by taking secretion of microbial cells as a matrix, belonging to the technical field of synthesis of nano-particles. The method for preparing selenium nano-particles by taking secretion of microbial cells as a matrix is technically characterized by comprising the following steps: inoculating 30-100mL of water with microbial single colonies and adding a sugary solution with mass concentration of 0.2-1.6%; oscillating for 24h, then adding 1-5mmol of selenium powder, selenic acid or sodium selenite, putting the mixed solution in a constant-temperature constant-speed shaker and oscillating for 3-24h at 20-60 DEG C at an oscillating speed of 100-600r/min; and centrifuging, collecting precipitate, washing and drying to obtain selenium nano-particles with particle size of 3-8nm. The method disclosed by the invention has the advantages of environment friendliness, good biocompatibility, simple preparation process and the like.
Description
Technical field
The invention belongs to nano science, microbiology, field of material synthesis technology, be specifically related to a kind of with the method for microorganism cells juice for matrix composition selenium nano particle.
Background technology
When size of particles enters nanometer scale, itself there is quantum size effect, surface effects, volume effect, macro quanta tunnel effect, Dielectric confinement effect, thus present a series of strange Wuli-Shili-Renli system approach, and relate to that ignore in body phase material or that do not have some basic physics, Chemical Problem.Therefore, nano material, as a kind of novel material, illustrates application prospect widely in information storage, catalysis, photoabsorption, luminescence, medicine and other fields.The fusing point of selenium is lower, and electric conductivity is higher, is a kind of important semiconductor material, is used to the fields such as solar cell, rectifier, toner cartridge, demonstrates unique character and wide application prospect.Selenium is also the trace element of needed by human, has important physiological function and pharmacological action widely.Selenium has certain checking and restraining effect to the generation of tumour, development.When the concentration of selenium is 0.04-0.1 μ g/g, being all useful to animal and human, is then harmful more than 4 μ g/g.Selenium is as a kind of important element semiconductor, and at photocell, rectifier, protect in sealed cell and xerox etc. and be all used widely, the nanostructure of its one dimension has unique function.The 1-dimention nano elemental selenium synthetic method reported has microemulsion method, ultrasonic method chemical method, solvent-thermal method etc., at production cost, solve in agglomeration traits, the simplicity of building-up process and purity etc. and have respective defect, can not reach balance, be not too satisfactory.As, the raw materials cost of microemulsion method is high, output is little, is difficult to realize suitability for industrialized production; Ultrasonic method chemical method, invests larger; The temperature of reaction of solvent-thermal method is high, long reaction time.And the application aspect of above synthetic method synthetic is easily by the impact of outside atmosphere.Still there is no a kind of novel production high-purity nm selenium technology up to now, in existing selenium production technology, also there is many problems, as in use poor reliability, factory cannot reach the requirement to envrionment conditions in experiment, and the environmental pollution that after producing selenium, metal ion waste water brings.Therefore, work out a kind of effective, do not affect purity and the quality of selenium, cost is low, production technique simple to operate, and to realize energy-saving and emission-reduction, the target of cleaner production is the important directions of producing selenium technical development.
Biosynthetic means can avoid Problems existing in many traditional chemical synthesis, such as on the impact of environment, the loss of energy, security and biocompatibility etc.; With microorganism cells juice for matrix, a kind of new experimental basis is provided to the research of biosynthetic means, also important reference and directive function are provided to the mechanism in research biosynthesizing.Utilize the material that organism is synthesized at present, the functional group of its outside that mainly utilizes of report made template in the past.So far there are no with microorganism is the report that matrix prepares the method for selenium nano particle therein.
Summary of the invention
The object of this invention is to provide a kind of with the method for microorganism cells juice for matrix composition selenium nano particle, the method is by biosynthetic method, effectively, safety, environmentally friendly carry out the preparation of selenium nano particle, obtained selenium nano particle diameter is homogeneous, size is little, good dispersity, Bc are good and reaction conditions is gentle, low in raw material price, the demand of eco-friendly commercial run and pollution-free product can be met.
The present invention adopts following technical scheme for achieving the above object, a kind of with the method for microorganism cells juice for matrix composition selenium nano particle, it is characterized in that concrete steps are: microbe inoculation list bacterium colony is in 30-100mL water, add the saccharide solution that mass concentration is 0.2%-1.6%, wherein microorganism is yeast, Fusarium oxysporum, lactobacillus, bifidus bacillus, diatom or blue-green algae, and carbohydrate is glucose, D-Maltose, sucrose or lentinan; Add the selenium powder of 1-5mmol, selenic acid or Sodium Selenite after concussion 24h, then mixing solutions is put into constant temperature constant speed shaking table, in 20-60 DEG C of concussion 3-24h, concussion speed is 100-600r/min; Centrifugal collecting precipitation, washing, drying obtains the selenium nano particle of particle diameter 3-8nm.
The present invention has following beneficial effect:
1, react and carry out in the aqueous solution and saccharide solution when microorganism participates in, mild condition, environmental friendliness;
2, be a kind of with low cost, production technique simple to operate;
The purity of the selenium nano particle 3, worked out is high, and quality is good;
4, a kind of new experimental basis is provided to the research of biosynthetic means, also important reference and directive function are provided to the mechanism in research biosynthesizing.
In a word, the present invention has environmental friendliness, the advantage such as the good and preparation process of Bc is simple.
Accompanying drawing explanation
Fig. 1 is the XRD figure spectrum of the selenium nano particle that the embodiment of the present invention 1 obtains, and Fig. 2 is the TEM figure of the selenium nano particle that the embodiment of the present invention 1 obtains.
specific embodiment
Be described in further details foregoing of the present invention by the following examples, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following embodiment, all technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
Yeast cell is cultivated: the single bacterium colony of inoculation yeast bacterium is in the water of 30mL, add the glucose solution that mass concentration is 0.2%, put into constant temperature constant speed shaking table to shake, pour the selenium powder of 1mM after concussion 24h fast into, in temperature 20 DEG C, concussion speed is 100r/min, concussion 3h, centrifuge washing, dry, the nano granules of selenium of obtained particle diameter 3-8nm.Fig. 1 is the XRD figure spectrum of the selenium nano particle that the present embodiment obtains, and product obtained is as seen from the figure composed consistent with the XRD figure of selenium simple substance, and Fig. 2 is the TEM figure of the selenium nano particle that the present embodiment obtains, and the particle diameter of selenium nano particle obtained is as seen from the figure 3-8nm.
Embodiment 2
Lactobacillus cell is cultivated: inoculating lactobacillus list bacterium colony, in the water of 40mL, adds the sucrose solution that mass concentration is 0.2%, put into constant temperature constant speed shaking table and shake, after concussion 24h, pour the Na of 1mM fast into
2seO
3the solution of preparation, in temperature 25 DEG C, concussion speed is 200r/min, concussion 5h, and centrifuge washing is dry, obtained particle diameter 3-8nm nano granules of selenium.
Embodiment 3
Yeast cell is cultivated: the single bacterium colony of inoculation yeast bacterium is in the water of 40mL, add the lentinan solution that mass concentration is 0.4%, put into constant temperature constant speed shaking table to shake, pour the solution of the selenic acid preparation of 2mM after concussion 24h fast into, in temperature 25 DEG C, concussion speed is 200r/min, concussion 5h, centrifuge washing, dry, the nano granules of selenium of obtained particle diameter 3-8nm.
Embodiment 4
Frustule is cultivated: the single bacterium colony of inoculation diatom is in the water of 40mL, add the sucrose solution that mass concentration is 0.4%, put into constant temperature constant speed shaking table to shake, pour the solution of the selenic acid preparation of 3mM after concussion 24h fast into, in temperature 30 DEG C, concussion speed is 300r/min, concussion 8h, centrifuge washing, dry, the nano granules of selenium of obtained particle diameter 3-8nm.
Embodiment 5
Lactobacillus cell is cultivated: inoculating lactobacillus list bacterium colony, in the water of 60mL, adds the glucose solution that mass concentration is 0.8%, put into constant temperature constant speed shaking table and shake, after concussion 24h, pour the Na of 3mM fast into
2seO
3the solution of preparation, in temperature 35 DEG C, concussion speed is 400r/min, concussion 16h, and centrifuge washing is dry, the nano granules of selenium of obtained particle diameter 3-8nm.
Embodiment 6
Bifidobacterium cells is cultivated: inoculating bifidobacterium list bacterium colony, in the water of 80mL, adds the lentinan solution that mass concentration is 1.0%, puts into constant temperature constant speed shaking table and shakes, after concussion 24h, pour the Na of 4mM fast into
2seO
3the solution of preparation, in temperature 40 DEG C, concussion speed is 400r/min, concussion 16h, and centrifuge washing is dry, the nano granules of selenium of obtained particle diameter 3-8nm.
Embodiment 7
By Fusarium oxysporum cell cultures: the single bacterium colony of inoculation Fusarium oxysporum is in the water of 80mL, add the glucose solution that mass concentration is 1.2%, put into constant temperature constant speed shaking table to shake, pour the solution of the selenic acid preparation of 5mM after concussion 24h fast into, in temperature 50 C, concussion speed is 500r/min, concussion 18h, centrifuge washing, dry, the nano granules of selenium of obtained particle diameter 3-8nm.
Embodiment 8
Cells of Blue-green Algae is cultivated: the single bacterium colony of inoculation blue-green algae is in the water of 100mL, add the D-Maltose solution that mass concentration is 1.6%, put into constant temperature constant speed shaking table to shake, pour the solution of the selenic acid preparation of 5mM after concussion 24h fast into, in temperature 60 C, concussion speed is 600r/min, concussion 24h, centrifuge washing, dry, the nano granules of selenium of obtained particle diameter 3-8nm.
Embodiment above describes ultimate principle of the present invention, principal character and advantage.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present invention; under the scope not departing from the principle of the invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the scope of protection of the invention.
Claims (1)
1. one kind with the method for microorganism cells juice for matrix composition selenium nano particle, it is characterized in that concrete steps are: microbe inoculation list bacterium colony is in 30-100mL water, add the saccharide solution that mass concentration is 0.2%-1.6%, wherein microorganism is yeast, Fusarium oxysporum, lactobacillus, bifidus bacillus, diatom or blue-green algae, and carbohydrate is glucose, D-Maltose, sucrose or lentinan; Add the selenium powder of 1-5mmol, selenic acid or Sodium Selenite after concussion 24h, then mixing solutions is put into constant temperature constant speed shaking table, in 20-60 DEG C of concussion 3-24h, concussion speed is 100-600r/min; Centrifugal collecting precipitation, washing, drying obtains the selenium nano particle of particle diameter 3-8nm.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039420A (en) * | 2015-07-23 | 2015-11-11 | 河南师范大学 | Method for preparing silicon hollow nano-spheres by aid of reducing agent which is secretion of microbial cells |
| CN105154474A (en) * | 2015-09-18 | 2015-12-16 | 宜春学院 | Biological preparation method of red nano selenium |
| CN105441489A (en) * | 2015-11-11 | 2016-03-30 | 大连理工大学 | Method for synthesizing selenium and bismuth selenide nano material by using lysinibacillus sp. |
| CN108676816A (en) * | 2018-05-04 | 2018-10-19 | 西北工业大学 | The method for preparing water-soluble nano selenium microballoon based on Lactobacillus casei L.casei 393 |
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| CN101454454A (en) * | 2006-05-22 | 2009-06-10 | 韩国科学技术院 | Method for preparing metal nanoparticles using metal binding proteins |
| CN102962467A (en) * | 2012-10-26 | 2013-03-13 | 上海交通大学 | Method for preparing noble metal nano material with adjustable particle size by bacteria |
| EP2315837B1 (en) * | 2008-08-21 | 2014-10-29 | National Research Council of Canada | Production of sulfur-free nanoparticles by saccharomyces |
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2015
- 2015-02-27 CN CN201510089257.3A patent/CN104745637A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101454454A (en) * | 2006-05-22 | 2009-06-10 | 韩国科学技术院 | Method for preparing metal nanoparticles using metal binding proteins |
| EP2315837B1 (en) * | 2008-08-21 | 2014-10-29 | National Research Council of Canada | Production of sulfur-free nanoparticles by saccharomyces |
| CN102962467A (en) * | 2012-10-26 | 2013-03-13 | 上海交通大学 | Method for preparing noble metal nano material with adjustable particle size by bacteria |
Non-Patent Citations (8)
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039420A (en) * | 2015-07-23 | 2015-11-11 | 河南师范大学 | Method for preparing silicon hollow nano-spheres by aid of reducing agent which is secretion of microbial cells |
| CN105039420B (en) * | 2015-07-23 | 2018-11-16 | 河南师范大学 | A method of silicon nano-hollow ball is prepared by reducing agent of microbial cell juice |
| CN105154474A (en) * | 2015-09-18 | 2015-12-16 | 宜春学院 | Biological preparation method of red nano selenium |
| CN105154474B (en) * | 2015-09-18 | 2018-08-10 | 宜春学院 | The biological preparation method of red nano selenium |
| CN105441489A (en) * | 2015-11-11 | 2016-03-30 | 大连理工大学 | Method for synthesizing selenium and bismuth selenide nano material by using lysinibacillus sp. |
| CN105441489B (en) * | 2015-11-11 | 2018-11-09 | 大连理工大学 | A method of synthesizing selenium and bismuth selenide nano material using lysine bacillus |
| CN108676816A (en) * | 2018-05-04 | 2018-10-19 | 西北工业大学 | The method for preparing water-soluble nano selenium microballoon based on Lactobacillus casei L.casei 393 |
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Application publication date: 20150701 |