CN104730011A - Microspectrophotometer - Google Patents
Microspectrophotometer Download PDFInfo
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- CN104730011A CN104730011A CN201510108765.1A CN201510108765A CN104730011A CN 104730011 A CN104730011 A CN 104730011A CN 201510108765 A CN201510108765 A CN 201510108765A CN 104730011 A CN104730011 A CN 104730011A
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Abstract
The invention discloses a microspectrophotometer. The microspectrophotometer comprises an upper measurement platform, a lower measurement platform, an adjustment rod and a distance induction mechanism, wherein the upper measurement platform and the lower measurement platform are opposite to each other, and the distance between the upper measurement platform and the lower measurement platform can be adjusted; a projecting optical fiber base and a receiving optical fiber base which are aligned to each other are respectively arranged on the upper measurement platform and the lower measurement platform; the adjustment rod is used for adjusting the distance between the upper measurement platform and the lower measurement platform and is driven by a driving device; the distance induction mechanism is used for controlling the distance between the upper measurement platform and the lower measurement platform, and the distance induction mechanism comprises an induction device and an induction component; the induction device comprises a transmitter and a receiver; the transmitter is capable of transmitting signals, and the receiver is capable of receiving signals under the barrier-free circumference; and the induction component can move into and out of the position between the transmitter and the receiver. The microspectrophotometer disclosed by the invention belongs to the technical field of microspectrophotometers and overcomes the technical problems of the existing microspectrophotometers. The existing micro-spectrophotometers can not automatically correct the changed optical path so that the detection result can be affected. The microspectrophotometer can be particularly used for automatically correcting the optical path to keep the length of the optical path constant and guarantee the accuracy of the sample detection result.
Description
Technical field
The invention belongs to spectrophotometer field, particularly a kind of micro-spectrophotometer.
Background technology
Micro-spectrophotometer can be widely used in the Quantitative detection of the samples such as sugar, nucleic acid, enzyme or albumen, when using micro-spectrophotometer to detect sample, it mainly utilizes the tension force traction of micro liquid to form light-path, so only need very micro-measuring samples namely can obtain detect data accurately, thus traditional use cuvette can be substituted completely detect, have novelty and practicality.
Existing micro-spectrophotometer, comprise the upper measuring table and lower measuring table that are connected by a central shaft, described upper measuring table can rotate around described central shaft, measuring table is provided with projection fibre seat on described, described lower measuring table is provided with reception fiber bench, sample bridge joint is between described projection fibre seat and described reception fiber bench, the adjuster bar of the spacing for regulating upper and lower measuring table is provided with between described lower measuring table and upper measuring table, described adjuster bar is driven by the motor be arranged on the downside of lower measuring table, be pressed on upper measuring table through lower measuring table top, during work, drive described motor, described adjuster bar is moved up and down, and then make the described upper measuring table of described adjuster bar pushing and pressing rotate up and down to adjust, distance between lower measuring table, realize the adjustment of optical path length between projection fibre seat and reception fiber bench.
When measuring different measuring samples owing to utilizing above-mentioned micro-spectrophotometer of the prior art, in order to obtain measurement result accurately, need to measure under a standard optical path length.And when practical application, after repeatedly sampling, distance between upper and lower measuring table can be worn and torn and the change of generation trace due to the Long-Time Service of the continuous open and close of upper measuring table and adjuster bar, that is photometric optical path length changes, now for ensureing that measurement result accurately needs to readjust, distance between lower measuring table, and at most also only have the distance of about 1mm due to the distance between upper and lower measuring table, utilize manual technique to carry out trace adjustment not only to need to require considerable time and effort, and degree of accuracy does not ensure completely, so just inevitably affect the accuracy of experimental result.If still will ensure detection accuracy after long-term use, user just has to seek the recovery that producer carries out Default Value.
Summary of the invention
For this reason, technical matters to be solved by this invention is that micro-spectrophotometer of the prior art is due to Long-Time Service, the trace change of optical path length when being easy to cause measurement, manual adjustments is utilized to be difficult to correct the change of this trace completely, if in order to the accuracy ensureing experimental result just has to carry out the recovery of Default Value, thus provide a kind of can automatic calibration optical path length thus make optical path length remain on the micro-spectrophotometer of a definite value.
For solving the problems of the technologies described above, a kind of micro-spectrophotometer of the present invention, it comprises and to be oppositely arranged and apart from adjustable upper measuring table and lower measuring table, described upper measuring table be provided with projection fibre seat or receive fiber bench, described lower measuring table is provided with and receives fiber bench or projection fibre seat, described projection fibre seat and described reception fiber bench are connected with optical fiber respectively, described projection fibre seat and described reception fiber bench align, up and down for holding measuring samples, for regulating the adjuster bar of the spacing of described upper measuring table and described lower measuring table, described adjuster bar is connected with drive unit, and moves up and down by described drive unit drives and push against described upper measuring table or described lower measuring table directly or indirectly, also comprise the distance induction mechanism of the spacing controlling described upper measuring table and described lower measuring table, described comprising apart from induction mechanism lays respectively on described upper measuring table and described lower measuring table and the induction installation be oppositely arranged and induction pieces, described induction installation has the transmitter and receiver that are oppositely arranged, described transmitter can send signal, and in without hindrance situation, described receiver can receive signal, described induction pieces can debouch between described transmitter and described receiver, when light path between described projection fibre seat and described reception fiber bench is preset initial value, the original state that one just can not block the signal that described transmitter sends is between described induction pieces and described induction installation, wear and tear between described adjuster bar and described upper measuring table or described lower measuring table, when causing light path to shorten, described induction pieces passes through between described transmitter and described receiver, the signal that described transmitter sends is blocked, described induction installation feedback control signal is to control module, control module controls described drive unit makes described adjuster bar move to regulate the distance between described upper measuring table and described lower measuring table, until make to return back to original state between described induction pieces and described induction installation, now light path returns to preset initial value.
Described induction installation comprises the induction installation body being fixedly connected on described upper measuring table or described lower measuring table, described induction installation body has atrium, described transmitter and described receiver lay respectively on the relative chamber wall of described atrium, described induction pieces corresponds to the inductive axis be connected on described lower measuring table or described upper measuring table, and described inductive axis extends to inside described induction installation body.
Described lower measuring table is fixedly installed, and one end away from described projection fibre seat or described reception fiber bench of described upper measuring table is pivotally connected on the hinge connector that is positioned on the upside of described lower measuring table.
Described induction pieces is arranged on described lower measuring table, and upper-lower position is adjustable.
Described transmitter and described receiver are arranged on described lower measuring table, and upper-lower position is adjustable.
Described adjuster bar is through described lower measuring table and to lean on described on measuring table.
Described drive unit is stepper motor, and one end that described adjuster bar coordinates with described stepper motor is screw-nut body.
Described induction installation is photoelectric sensor, and the signal that described transmitter sends is infrared light or visible ray.
Technique scheme of the present invention has the following advantages compared to existing technology:
(1) a kind of micro-spectrophotometer of the present invention, be provided with the distance induction mechanism of the spacing controlling upper measuring table and lower measuring table, described distance induction mechanism is by being arranged at the induction installation of upper measuring table or lower measuring table, the corresponding induction pieces being arranged at lower measuring table or upper measuring table of induction, control and limit the distance between described upper measuring table and described lower measuring table, and regulating the distance between upper and lower measuring table by the adjuster bar of conflicting on upper measuring table or lower measuring table; Thus the impact of wearing and tearing between described adjuster bar and described upper measuring table can not be subject to.The automatic calibration of optical path length can be realized, without the need to carrying out the recovery of Default Value, optical path length can be made to remain on preset initial value, thus guarantee the accuracy of sample detection result.
(2) in micro-spectrophotometer of the present invention, described induction pieces is arranged on described lower measuring table, and upper-lower position is adjustable, by the initial position regulating the upper-lower position of described induction pieces to change described induction installation and described upper measuring table, and then change the preset initial value of light path between described projection fibre seat and described reception fiber bench, the change realized preset initial value as required, carry out sample detection under the present invention can be made to be supported in different predetermined optical path lengths, range of application is more extensive.
(3) in micro-spectrophotometer of the present invention, described drive unit is stepper motor, one end that described adjuster bar coordinates with described stepper motor is screw-nut body, and this fit system makes the adjustment of described adjuster bar to the distance between described upper measuring table and described lower measuring table more accurate.
Accompanying drawing explanation
In order to make content of the present invention be more likely to be clearly understood, below according to a particular embodiment of the invention and by reference to the accompanying drawings, the present invention is further detailed explanation, wherein
Fig. 1 is the stereographic map of measuring table open mode on micro-spectrophotometer of the present invention;
Fig. 2 is the vertical view of measuring table open mode on micro-spectrophotometer of the present invention;
Fig. 3 is the cut-open view of measuring table open mode on micro-spectrophotometer of the present invention;
Fig. 4 is the stereographic map of measuring table closure state on micro-spectrophotometer of the present invention;
Fig. 5 is the vertical view of measuring table closure state on micro-spectrophotometer of the present invention;
Fig. 6 is the cut-open view of measuring table closure state on micro-spectrophotometer of the present invention;
Fig. 7 is the front view of measuring table closure state on micro-spectrophotometer of the present invention.
In figure, Reference numeral is expressed as: the upper measuring table of 1-, measuring table under 2-, 21-hinge connector, 3-projection fibre seat, and 4-receives fiber bench, 5-induction installation, 50-induction installation body, 501-atrium, 51-transmitter, 52-receiver, 6-induction pieces, 7-adjuster bar, 8-stepper motor.
Embodiment
Below with reference to accompanying drawing, following examples are used to be further elaborated the present invention.
Consult Fig. 1, Fig. 2 and Fig. 4, Fig. 5, a kind of micro-spectrophotometer of the present invention, comprise measuring table 1, lower measuring table 2, adjuster bar 7 and distance induction mechanism.
Wherein, consult Fig. 3, Fig. 6 and Fig. 7, described upper measuring table 1 and lower measuring table 2 are oppositely arranged and distance is between the two adjustable, described upper measuring table 1 is provided with projection fibre seat 3, described lower measuring table 2 is provided with and receives fiber bench 4, described projection fibre seat 3 and described reception fiber bench 4 are connected with optical fiber respectively; Described projection fibre seat 3 and described reception fiber bench about 4 align, for holding measuring samples;
Described adjuster bar 7 is connected with drive unit, and moves up and down by described drive unit drives and push against described upper measuring table 1 directly or indirectly, for regulating the distance between described upper measuring table 1 and described lower measuring table 2;
Described comprising apart from induction mechanism lays respectively on described upper measuring table 1 and described lower measuring table 2 and the induction installation 5 be oppositely arranged and induction pieces 6, described induction installation 5 has the transmitter 51 and receiver 52 that are oppositely arranged, transmitter 51 can send signal, and in without hindrance situation, receiver 52 can receive signal; Described induction pieces 6 can debouch between described transmitter 51 and described receiver 52, and described distance induction mechanism is for controlling the distance between described upper measuring table 1 and described lower measuring table 2;
When light path between described projection fibre seat 3 and described reception fiber bench 4 is preset initial value, the original state that one just can not block the signal that described transmitter 51 sends is between described induction pieces 6 and described induction installation 5, wear and tear between described adjuster bar 7 and described upper measuring table 1, when causing light path to shorten, described induction pieces 6 passes through between described transmitter 51 and described receiver 52, the signal that described transmitter 51 sends is blocked, described induction installation 5 feedback control signal is to control module, control module controls described drive unit makes described adjuster bar 7 move to regulate the distance between described upper measuring table 1 and described lower measuring table 2, until make to return back to original state between described induction pieces 6 and described induction installation 5, now light path returns to preset initial value.
Such scheme is core scheme of the present invention, this micro-spectrophotometer is responded to described induction pieces 6 by described induction installation 5 and is controlled and limit the distance between described upper measuring table 1 and described lower measuring table 2, thus can not be subject to the impact of wearing and tearing between described adjuster bar 7 and described upper measuring table 1.The automatic calibration of optical path length can be realized, without the need to carrying out the recovery of Default Value, optical path length can be made to remain on preset initial value, thus guarantee the accuracy of sample detection result.
Particularly, described lower measuring table 2 is fixedly installed, and one end away from described projection fibre seat 3 of described upper measuring table 1 is pivotally connected on the hinge connector 21 that is positioned on the upside of described lower measuring table 2.Described upper measuring table 1 can realize described projection fibre seat 3 and aligns with described reception fiber bench about 4 or be separated by the pivot axis on hinge connector 21.
The concrete structure of described induction installation 5 and described induction pieces 6 can be various ways, as shown in Figure 3, in the present embodiment, described induction installation 5 comprises the induction installation body 50 being fixedly connected on described upper measuring table 1, described induction installation body 50 has atrium 501, described transmitter 51 and described receiver 52 lay respectively on the relative chamber wall of described atrium 501, described induction pieces 6 corresponds to the inductive axis be connected on described lower measuring table 2, and described inductive axis extends to inside described induction installation body 50.The installation site of described induction installation 5 and described induction pieces 6 can also exchange, and namely induction installation 5 is installed on lower measuring table 2, and induction pieces 6 is installed on the first measuring table.This distance perspective answers mechanism structure simple, easy for installation.
Described preset initial value can be predisposed to different values as required, the following two kinds mode can be adopted realize: the first is that described transmitter 51 is fixedly installed on described upper measuring table 1 with described receiver 52, described induction pieces 6 is arranged on described lower measuring table 2, and upper-lower position is adjustable, by the initial position regulating the upper-lower position of described induction pieces 6 to change described induction installation 5 and described upper measuring table 1, and then change the preset initial value of light path between described projection fibre seat 3 and described reception fiber bench 4; The second is that described induction pieces 6 is fixedly installed on described upper measuring table 1, described transmitter 51 is arranged on described lower measuring table 2 with described receiver 52, and upper-lower position is adjustable, by the initial position regulating described transmitter 51 and the upper-lower position of described receiver 52 to change described induction pieces 6 and described upper measuring table 1, and then change the preset initial value of light path between described projection fibre seat 3 and described reception fiber bench 4.Wherein, in the present embodiment, if when needing to change preset initial value, preferred first kind of way.The change realized preset initial value as required, carry out sample detection under the present invention can be made to be supported in different predetermined optical path lengths, range of application is more extensive.
Described adjuster bar 7 is through described lower measuring table 2 and to lean on described on measuring table 1.The described drive unit be connected with described adjuster bar 7 is positioned at below described lower measuring table 2.
Described adjuster bar 7 has multiple with the fit system of described drive unit, and in the present embodiment, preferred described drive unit is stepper motor 8, and one end that described adjuster bar 7 coordinates with described stepper motor 8 is screw-nut body.This fit system makes the adjustment of described adjuster bar 7 to the distance between described upper measuring table 1 and described lower measuring table 2 more accurate.
Further, described induction installation 5 is photoelectric sensor, and the signal that described transmitter 51 sends is infrared light or visible ray.
The process of the automatic calibration optical path length of a kind of micro-spectrophotometer of the present invention is as follows:
First, the original state that one just can not block the signal that described transmitter 51 sends is between described induction pieces 6 and described induction installation 5, now described upper measuring table 1 is pushed against by described adjuster bar 7 and keeps the distance between described lower measuring table 2, light path between described projection fibre seat 3 and described reception fiber bench 4 is preset initial value
Then, when there are wearing and tearing due to Long-Time Service between described adjuster bar 7 and described upper measuring table 1, distance between described upper measuring table 1 and described lower measuring table 2 can shorten, thus cause light path to shorten, now, described induction pieces 6 can pass through between described transmitter 51 and described receiver 52, the signal that described transmitter 51 sends is blocked, described induction installation 5 feedback control signal is to control module, control module controls described stepper motor 8 makes described adjuster bar 7 move to regulate the distance between described upper measuring table 1 and described lower measuring table 2, until make to return back to original state between described induction pieces 6 and described induction installation 5, described induction installation 5 is just responded to less than described induction pieces 6, and then feed back to and control signal to control module, control module controls described stepper motor 8 and quits work, described adjuster bar 7 stops mobile, because described lower measuring table 2 is motionless, distance between described upper measuring table 1 and described lower measuring table 2 is decided by the rotation of described upper measuring table 1, and the rotation of described upper measuring table 1 is responded to described induction pieces 6 by described induction installation 5 and is controlled, therefore, as long as the length that described induction pieces 6 stretches out described lower measuring table 2 part is constant, distance between described upper measuring table 1 and described lower measuring table 2 finally would not change, so the light path now between described projection fibre seat 3 and described reception fiber bench 4 also can return to preset initial value.
Obviously, above-described embodiment is only for clearly example being described, and the restriction not to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.And thus the apparent change of extending out or variation be still among the protection domain of the invention.
Claims (8)
1. a micro-spectrophotometer, it comprises
To be oppositely arranged and apart from adjustable upper measuring table (1) and lower measuring table (2), described upper measuring table (1) be provided with projection fibre seat (3) or receive fiber bench (4), described lower measuring table (2) is provided with and receives fiber bench (4) or projection fibre seat (3), described projection fibre seat (3) and described reception fiber bench (4) are connected with optical fiber respectively; Described projection fibre seat (3) and described reception fiber bench (4) align, up and down for holding measuring samples;
For regulating the adjuster bar (7) of the spacing of described upper measuring table (1) and described lower measuring table (2), described adjuster bar (7) is connected with drive unit, and moves up and down by described drive unit drives and push against described upper measuring table (1) or described lower measuring table (2) directly or indirectly;
It is characterized in that:
Also comprise the distance induction mechanism of the spacing controlling described upper measuring table (1) and described lower measuring table (2), described comprising apart from induction mechanism lays respectively on described upper measuring table (1) and described lower measuring table (2) and the induction installation be oppositely arranged (5) and induction pieces (6), described induction installation (5) has the transmitter (51) and receiver (52) that are oppositely arranged, described transmitter (51) can send signal, and in without hindrance situation, described receiver (52) can receive signal; Described induction pieces (6) can debouch between described transmitter (51) and described receiver (52);
When light path between described projection fibre seat (3) and described reception fiber bench (4) is preset initial value, the original state that one just can not block the signal that described transmitter (51) sends is between described induction pieces (6) and described induction installation (5), wear and tear between described adjuster bar (7) and described upper measuring table (1) or described lower measuring table (2), when causing light path to shorten, described induction pieces (6) passes through between described transmitter (51) and described receiver (52), the signal that described transmitter (51) sends is blocked, described induction installation (5) feedback control signal is to control module, control module controls described drive unit and makes the mobile distance regulated between described upper measuring table (1) and described lower measuring table (2) of described adjuster bar (7), until make to return back to original state between described induction pieces (6) and described induction installation (5), now light path returns to preset initial value.
2. a kind of micro-spectrophotometer according to claim 1, it is characterized in that: described induction installation (5) comprises the induction installation body (50) being fixedly connected on described upper measuring table (1) or described lower measuring table (2), described induction installation body (50) has atrium (501), described transmitter (51) and described receiver (52) lay respectively on the relative chamber wall of described atrium (501), described induction pieces (6) corresponds to the inductive axis be connected on described lower measuring table (2) or described upper measuring table (1), described inductive axis extends to described induction installation body (50) inner side.
3. a kind of micro-spectrophotometer according to claim 1 and 2, it is characterized in that: described lower measuring table (2) is fixedly installed, one end away from described projection fibre seat (3) or described reception fiber bench (4) of described upper measuring table (1) be pivotally connected to be positioned at described lower measuring table (2) upside hinge connector (21) on.
4. a kind of micro-spectrophotometer according to claim 3, is characterized in that: described induction pieces (6) is arranged on described lower measuring table (2), and upper-lower position is adjustable.
5. a kind of micro-spectrophotometer according to claim 3, is characterized in that: described transmitter (51) and described receiver (52) are arranged on described lower measuring table (2), and upper-lower position is adjustable.
6. according to the arbitrary described a kind of micro-spectrophotometer of claim 1-5, it is characterized in that: described adjuster bar (7) is through described lower measuring table (2) and to lean on described on measuring table (1).
7. according to the arbitrary described a kind of micro-spectrophotometer of claim 1-6, it is characterized in that: described drive unit is stepper motor (8), one end that described adjuster bar (7) coordinates with described stepper motor (8) is screw-nut body.
8., according to the arbitrary described a kind of micro-spectrophotometer of claim 1-7, it is characterized in that: described induction installation (5) is photoelectric sensor, the signal that described transmitter (51) sends is infrared light or visible ray.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510108765.1A CN104730011B (en) | 2015-03-12 | 2015-03-12 | A kind of micro-spectrophotometer |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510108765.1A CN104730011B (en) | 2015-03-12 | 2015-03-12 | A kind of micro-spectrophotometer |
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| CN104730011A true CN104730011A (en) | 2015-06-24 |
| CN104730011B CN104730011B (en) | 2018-09-07 |
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| CN201510108765.1A Active CN104730011B (en) | 2015-03-12 | 2015-03-12 | A kind of micro-spectrophotometer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105547473A (en) * | 2016-01-19 | 2016-05-04 | 济南大学 | Spectrophotometer |
| CN110702629A (en) * | 2019-11-14 | 2020-01-17 | 嘉兴领宇科技有限公司 | Spectrophotometer |
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| CN2847269Y (en) * | 2005-07-18 | 2006-12-13 | 杭州华大基因研发中心 | Support frame structure for liquid sample detector |
| EP1792653A1 (en) * | 2005-12-05 | 2007-06-06 | FOSS Analytical A/S | Apparatus and method for spectrophotometric analysis |
| JP2012504767A (en) * | 2008-10-03 | 2012-02-23 | ナノドロップ テクノロジーズ リミテッド ライアビリティ カンパニー | Dual sample mode spectrophotometer |
| CN204556493U (en) * | 2015-03-12 | 2015-08-12 | 邓少铭 | A kind of micro-spectrophotometer |
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2015
- 2015-03-12 CN CN201510108765.1A patent/CN104730011B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2847269Y (en) * | 2005-07-18 | 2006-12-13 | 杭州华大基因研发中心 | Support frame structure for liquid sample detector |
| EP1792653A1 (en) * | 2005-12-05 | 2007-06-06 | FOSS Analytical A/S | Apparatus and method for spectrophotometric analysis |
| JP2012504767A (en) * | 2008-10-03 | 2012-02-23 | ナノドロップ テクノロジーズ リミテッド ライアビリティ カンパニー | Dual sample mode spectrophotometer |
| JP5539367B2 (en) * | 2008-10-03 | 2014-07-02 | ナノドロップ テクノロジーズ リミテッド ライアビリティ カンパニー | Dual sample mode spectrophotometer |
| CN204556493U (en) * | 2015-03-12 | 2015-08-12 | 邓少铭 | A kind of micro-spectrophotometer |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105547473A (en) * | 2016-01-19 | 2016-05-04 | 济南大学 | Spectrophotometer |
| CN110702629A (en) * | 2019-11-14 | 2020-01-17 | 嘉兴领宇科技有限公司 | Spectrophotometer |
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| Publication number | Publication date |
|---|---|
| CN104730011B (en) | 2018-09-07 |
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