CN104726348A - Slant test-tube culture medium for aspergillus candidus strain - Google Patents
Slant test-tube culture medium for aspergillus candidus strain Download PDFInfo
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- CN104726348A CN104726348A CN201510139042.8A CN201510139042A CN104726348A CN 104726348 A CN104726348 A CN 104726348A CN 201510139042 A CN201510139042 A CN 201510139042A CN 104726348 A CN104726348 A CN 104726348A
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- culture medium
- aspergillus candidus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
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Abstract
The invention discloses a slant test-tube culture medium for an aspergillus candidus strain. The slant test-tube culture medium comprises the following raw materials: 3g of sodium nitrate, 1g of dipotassium phosphate, 0.5g of magnesium sulphate, 0.5g of potassium chloride, 0.01g of ferrous sulphate, 30g of saccharose, 20g of agar and 1000mL of distilled water; all the raw materials are mixed and heated to be dissolved, sub-packaged and then sterilized for 20min at 121 DEG C; condensed water on a teat-tube wall is dried, and then the sterilized mixture is taken out and a slant is placed in a test tube, the sterilized mixture is solidified and then cultured for 48h in an incubator at 30 DEG C. The culture medium is beneficial to culture for the aspergillus candidus strain, and the cultured aspergillus candidus strain is high in quality.
Description
Technical field
The present invention relates to white wine field, specifically a kind of Ha Noi aspergillus candidus kind slant tube substratum.
Background technology
Research Literature about Shochu koji is a lot, and brief introduction of now making a summary is as follows:
Shochu koji is mould is that former one of Ha Noi was separated from the bubble Sheng aspergillus niger of each liquor factory in deer island use in 1918.It is said that white song is cultivated on bread by he at that time, be placed in calcium chloride moisture eliminator, halmatogenesis occurs.So-called white bent, real is interior osmanthus look, and at that time in order to be different from black song, called after was bent in vain then.The rich bent brewing white spirit of distillery of Fushun City of China test white wine that aims at of nineteen forty-two black pine succeeds, and in Liaoning Province, a distillery extensively adopts.1949 after liberation, and northeast sale monopoly general bureau three province's popularity northeastward, in recent years again by outstanding black aspergillus kind is replaced.1949, the male coomb's test Coomb of northern former feel proved, Aspergillus albicans and aspergillus niger, and except color, form is substantially identical.But aspergillus niger is contained and different with bubble by mould system, be a kind of new bacterium completely, therefore called after Ha Noi is bent in vain, and admitted by academia.
Summary of the invention
The object of the present invention is to provide a kind of Ha Noi aspergillus candidus kind slant tube substratum.
For achieving the above object, the invention provides following technical scheme:
A kind of Ha Noi aspergillus candidus kind slant tube substratum, raw material is: SODIUMNITRATE 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, Repone K 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 20g, distilled water 1000mL; All raw material Hybrid Heating are dissolved, 121 DEG C of sterilizing 20min after packing; Water of condensation on test tube wall is dried, then the mixture after sterilizing is taken out, and put inclined-plane in vitro, solidify to be placed in 30 DEG C of incubators and cultivate 48h.
Compared with prior art, the invention has the beneficial effects as follows: this substratum is conducive to the cultivation of Ha Noi aspergillus candidus kind, the quality of the Ha Noi aspergillus candidus kind of turning out is high.
Embodiment
Be described in more detail below in conjunction with the technical scheme of embodiment to this patent.
A kind of Ha Noi aspergillus candidus kind slant tube substratum, raw material is: SODIUMNITRATE 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, Repone K 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 20g, distilled water 1000mL; All raw material Hybrid Heating are dissolved, 121 DEG C of sterilizing 20min after packing; Water of condensation on test tube wall is dried, then the mixture after sterilizing is taken out, and put inclined-plane in vitro, solidify to be placed in 30 DEG C of incubators and cultivate 48h.
Above the better embodiment of this patent is explained in detail, but this patent is not limited to above-mentioned embodiment, in the ken that one skilled in the relevant art possesses, can also makes a variety of changes under the prerequisite not departing from this patent aim.
Claims (1)
1. a Ha Noi aspergillus candidus kind slant tube substratum, is characterized in that, raw material is: SODIUMNITRATE 3g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.5g, Repone K 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 20g, distilled water 1000mL; All raw material Hybrid Heating are dissolved, 121 DEG C of sterilizing 20min after packing; Water of condensation on test tube wall is dried, then the mixture after sterilizing is taken out, and put inclined-plane in vitro, solidify to be placed in 30 DEG C of incubators and cultivate 48h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510139042.8A CN104726348A (en) | 2015-03-27 | 2015-03-27 | Slant test-tube culture medium for aspergillus candidus strain |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510139042.8A CN104726348A (en) | 2015-03-27 | 2015-03-27 | Slant test-tube culture medium for aspergillus candidus strain |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104726348A true CN104726348A (en) | 2015-06-24 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510139042.8A Pending CN104726348A (en) | 2015-03-27 | 2015-03-27 | Slant test-tube culture medium for aspergillus candidus strain |
Country Status (1)
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| CN (1) | CN104726348A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132233A (en) * | 2015-07-24 | 2015-12-09 | 济宁医学院 | Novel aspergillus candidus production process |
| CN106947701A (en) * | 2017-04-13 | 2017-07-14 | 山东梁山徐坊大曲有限公司 | The method for improving the white bent saccharifying power in Ha Noi |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103642695A (en) * | 2013-08-28 | 2014-03-19 | 浙江工业大学 | Strain of Aspergillus oryzae and applications in preparation of feed additives through microbial fermentation |
| CN104232401A (en) * | 2014-09-02 | 2014-12-24 | 丹江口市曲之源生物科技有限公司 | Distiller's yeast and production method thereof |
-
2015
- 2015-03-27 CN CN201510139042.8A patent/CN104726348A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103642695A (en) * | 2013-08-28 | 2014-03-19 | 浙江工业大学 | Strain of Aspergillus oryzae and applications in preparation of feed additives through microbial fermentation |
| CN104232401A (en) * | 2014-09-02 | 2014-12-24 | 丹江口市曲之源生物科技有限公司 | Distiller's yeast and production method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 王叔淳: "（七十七）察氏培养基", 《食品卫生检验技术手册（第三版）》 * |
| 苏世彦: "十三、察氏培养基", 《食品微生物检验手册》 * |
| 许玲: "河内白曲最适培养条件研究", 《酿酒科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132233A (en) * | 2015-07-24 | 2015-12-09 | 济宁医学院 | Novel aspergillus candidus production process |
| CN105132233B (en) * | 2015-07-24 | 2017-07-14 | 济宁医学院 | A kind of white bent production technology in Ha Noi |
| CN106947701A (en) * | 2017-04-13 | 2017-07-14 | 山东梁山徐坊大曲有限公司 | The method for improving the white bent saccharifying power in Ha Noi |
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| Date | Code | Title | Description |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C53 | Correction of patent of invention or patent application | ||
| CB03 | Change of inventor or designer information |
Inventor after: Wu Guangqian Inventor after: Huang Yongguang Inventor after: Ma Zongjie Inventor after: Liu Bo Inventor after: Guo Qipeng Inventor after: Yang Guohua Inventor before: Wu Guangju Inventor before: Huang Yongguang Inventor before: Ma Zongjie Inventor before: Liu Bo Inventor before: Guo Qipeng Inventor before: Yang Guohua |
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| COR | Change of bibliographic data |
Free format text: CORRECT: INVENTOR; FROM: WU GUANGJU HUANG YONGGUANG MA ZONGJIE LIU BO GUO QIPENG YANG GUOHUA TO: WU GUANGQIAN HUANG YONGGUANG MA ZONGJIE LIU BO GUO QIPENG YANG GUOHUA |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150624 |
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| RJ01 | Rejection of invention patent application after publication |