CN104720929A - Mouse early embryo acquisition device and method - Google Patents

Abstract

The invention discloses a mouse early embryo acquisition device and method. The mouse early embryo acquisition device comprises an outer barrel, an inner barrel, a center rod, a needle board and a porous board. The outer barrel is a hollow barrel and the lower portion of the outer barrel is provided with a circular table with the diameter larger than that of the upper portion. The inner barrel is arranged in an inner cavity of the outer barrel, a sliding handle protruding out of a strip-shaped slot in the side wall of the outer barrel is arranged at the upper end of the inner barrel, and the lower end of the inner barrel is connected with the needle board arranged in an inner cavity of the circular table. The center rod is arranged in an inner cavity of the inner barrel, the upper end of the center rod extends out of an open portion of the upper end of the outer barrel and is connected with a handle, and the lower end of the center rod penetrates through a center hole of the needle board and is connected with the porous board in the inner cavity of the circular table. The needle board and the porous board share the same center axis and the needle board is located above the porous board. The external diameter of the needle board and the external diameter of the porous board are matched with the internal diameter of the circular table at the lower portion of the outer barrel. Multiple puncture needles extending downwards are arranged on the needle board. The porous board is provided with holes corresponding to the puncture needles in position and matched with the puncture needles in diameter. By the adoption of the device and method, a large number of early free embryos can be acquired quickly and efficiently from the fallopian tube or the uterine horn of a mouse.

Description

Mouse Embryos acquisition device and acquisition methods

Technical field

The present invention relates to a kind of Mouse Embryos acquisition device and acquisition methods.

Background technology

Reproduction and health are the themes of the world today, have left reproduction activity and will not exist at any species of occurring in nature, particularly particularly important for human colony's prenatal and postnatal care and healthy benign development.Therefore, for the research of reproduction aspect be focus and the difficult point of current life science.For the correlational study of human reproduction's health, experiment mice is then the ideal animals model of human research's reproduction phenomenon.Early stage embryo exists with free state mice, the embryo of these free states is taken out from reproductive tract, growth and development characteristics and the rule of body early embryo can be studied well, thus establish solid theory and practice basis for the prenatal and postnatal care of newborn individual.

Because Mouse Oviductal is the bulk structure formed by bending tubule, cornua uteri one section of compact siro spinning technology fallopian tube one section.At present, be artificial mechanical from Mouse Oviductal or the early stage free embryo of cornua uteri acquisition and peel off acquisition, owing to lacking acquisition equipment or the device of specialty, cause embryo's acquisition inefficiency of dissociating in early days, bothersome effort.

There is following problem in Traditional Man machinery acquisition methods:

1, at substantial manpower and the time, the time progressively puncturing fallopian tube needs is long;

2, method is not easy to operate, and fallopian tube is more bending, and every section of requirement all punctures and do not reach good effect;

3, acquisition time is longer, it is comparatively unfavorable to grow body early embryo further growth, in acquisition process, embryo is at extraneous overstand, when particularly fallopian tube punctures completely, the embryo that may sting out is the earliest dead or degeneration, even if do not have death, remaining mass also can be a greater impact, and later stage growth can produce some infringements and cause arrest of development in growth course.

For above problems, in production practices, design invents Mouse Embryos acquisition device.

Summary of the invention

The technical problem to be solved in the present invention is to provide a kind of Mouse Embryos acquisition device.

The another one technical problem that the present invention will solve is to provide a kind of Mouse Embryos acquisition methods.

For Mouse Embryos acquisition device, the technical solution used in the present invention is: Mouse Embryos acquisition device, comprises urceolus, inner core, center-pole, needle plate, hole plate;

Urceolus is hollow cylinder, and bottom is provided with the round platform that diameter is greater than upper diameter;

Inner core is placed in outer tube inner chamber, and inner core upper end is provided with the sliding handle protruding from bar shape trench hole on outer tube side wall, and inner core lower end is connected with the needle plate being placed in urceolus bottom round platform inner chamber; Center-pole is placed in the inner chamber of inner core, and the upper end of center-pole is stretched out from urceolus upper end tapping and is connected with lever, and center-pole lower end is connected with the hole plate being placed in urceolus bottom round platform inner chamber through the medium pore of needle plate;

Needle plate and hole plate are same central shaft and needle plate is positioned at the top of hole plate; The round platform internal diameter of needle plate and hole plate external diameter and urceolus bottom is suitable;

The pricker that needle plate is stretched under being provided with complex root, hole plate is provided with corresponding with needle positions and that diameter is suitable hole.

As preferably, the side on urceolus top is provided with and hooks shape handle.

As preferably, urceolus bottom round platform internal diameter is 10 ~ 15mm.

As preferably, on the plate of hole, hole diameter is 1.5 ~ 2mm.

As preferably, urceolus, inner core, center-pole, needle plate, hole plate adopt rustless steel, alloy or plastic material to make.

For Mouse Embryos acquisition methods, the technical solution used in the present invention is: comprise the following steps:

(1) surrounding of the Mouse Oviductal under external ex vivo situation or cornua uteri is surrounded and is fixed in platen surface;

(2) Mus fallopian tube or cornua uteri tube wall is punctured downwards with pin;

(3) oppress Mouse Oviductal or cornua uteri from the top down, make free shape Mouse Embryos puncture position from Mouse Oviductal or cornua uteri and overflow, then collect embryo.

The invention has the beneficial effects as follows:

A large amount of early stage free embryo can be obtained, for the research of reproductive development aspect in quickness and high efficiency ground from Mouse Oviductal or cornua uteri.

Accompanying drawing explanation

Below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation.

Fig. 1 is the profile schematic perspective view of Mouse Embryos acquisition device embodiment of the present invention.

Fig. 2 is the perspective view of Mouse Embryos acquisition device embodiment of the present invention.

Fig. 3 is the sectional view of Mouse Embryos acquisition device embodiment of the present invention.

Labelling in figure, 1-center-pole, 2-button, 3-hooks shape handle, 4-bar-shaped trough, 5-sliding handle, 6-urceolus, 7-inner core, 8-round platform, 9-needle plate, 10-hole plate, 11-pricker, 12-hole.

Detailed description of the invention

Fig. 1 is a kind of Mouse Embryos acquisition device, is made up of urceolus 6, inner core 7, center-pole 1, needle plate 9, hole plate 10.

In fig. 2, urceolus 6 is hollow cylinder, and bottom is provided with the round platform 8 that diameter is greater than upper diameter.

Inner core 7 is placed in outer tube inner chamber, and inner core 7 upper end is provided with the sliding handle 5 protruding from bar shape trench hole on outer tube side wall, and inner core 7 lower end is connected with the needle plate 9 being placed in urceolus lower lumen.Center-pole is placed in the inner chamber of inner core, and the upper end of center-pole is stretched out from urceolus upper end tapping and is connected with lever, and center-pole lower end is connected with the hole plate being placed in urceolus lower lumen through the medium pore of needle plate.

In figure 3, needle plate 9 and hole plate 10 are same central shaft, and needle plate is positioned at the top of hole plate.The internal diameter of the external diameter of needle plate 9 and hole plate 10 and the round platform 8 of urceolus 6 bottom is suitable, and needle plate 9 and hole plate 10 can slide at round platform inner chamber.

Needle plate is fixed with the pricker 11 stretched under tens of, hole plate is provided with and hole 12 that diameter suitable corresponding with pricker 11 position.

Be provided with in the side on urceolus top and hook shape handle 3.

Urceolus, inner core, center-pole, needle plate, hole plate adopt rustless steel, alloy or plastic material to make.

Each component effect and using method:

Whole components of whole embryo's acquisition device are assemblied in outer tube inner chamber, and a hook shape handle 3 is fixed in the upper end of urceolus, holds embryo's acquisition device urceolus during use, to fix and to prevent landing.

Sliding handle 5 exposes to outer tube side wall, is easy to operation; Sliding handle 5 is connected with needle plate 9 by inner core, and sliding handle slides up and down for controlling needle plate 9.

Urceolus 6 top is the pipe that diameter is less, and bottom is the round platform 8 that diameter is larger, and its internal diameter is 10 ~ 15mm, and the inner chamber of round platform can be covered on isolated mouse fallopian tube or cornua uteri wherein.

Needle plate 9 is fixed with dozens of pricker 11, every root thorn pin is corresponding with the hole 12 on hole plate 10, and pricker 11 is for puncturing the fallopian tube or cornua uteri tube wall that cover on round platform inner chamber.

Hole plate 10 has dozens of diameter be the circular opening 12 of 1.5 ~ 2mm, and with pricker on needle plate 11 one_to_one corresponding, its effect applies pressure by pressing down the embryo in fallopian tube or cornua uteri is overflowed by tube wall puncturing position, then collects embryo.

During use, in vitro round platform inner chamber covers Mouse Oviductal or cornua uteri in vitro, pressing sliding handle makes needle plate move down, puncture and fix Mouse Oviductal or cornua uteri with the pricker on needle plate, then push button 2, applying pressure by hole plate slide downward the embryo in fallopian tube or cornua uteri is overflowed by tube wall puncturing position, then collecting embryo.

Above-described embodiment of the present invention, does not form limiting the scope of the present invention.Any amendment done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within claims of the present invention.

Claims (6)

1. Mouse Embryos acquisition device, is characterized in that: comprise urceolus, inner core, center-pole, needle plate, hole plate;

Described urceolus is hollow cylinder, and bottom is provided with the round platform that diameter is greater than upper diameter;

Described inner core is placed in outer tube inner chamber, and described inner core upper end is provided with the sliding handle protruding from bar shape trench hole on outer tube side wall, and inner core lower end is connected with the needle plate being placed in urceolus bottom round platform inner chamber; Described center-pole is placed in the inner chamber of inner core, and the upper end of center-pole is stretched out from urceolus upper end tapping and is connected with lever, and center-pole lower end is connected with the hole plate being placed in urceolus bottom round platform inner chamber through the medium pore of needle plate;

Described needle plate and hole plate are same central shaft and needle plate is positioned at the top of hole plate; The external diameter of needle plate, hole plate and the round platform lower inner diameter of urceolus suitable;

The pricker that described needle plate is stretched under being provided with complex root, described hole plate is provided with corresponding with needle positions and that diameter is suitable hole.

2. acquisition device according to claim 1, is characterized in that: the side on described urceolus top is provided with and hooks shape handle.

3. acquisition device according to claim 1, is characterized in that: described urceolus bottom round platform internal diameter is 10 ~ 15mm.

4. acquisition device according to claim 1, is characterized in that: on the plate of described hole, hole diameter is 1.5 ~ 2mm.

5. acquisition device according to claim 1, is characterized in that: described urceolus, inner core, center-pole, needle plate, hole plate adopt rustless steel, alloy or plastic material to make.

6. Mouse Embryos acquisition methods, is characterized in that comprising the following steps:

(1) Mouse Oviductal under external ex vivo situation or cornua uteri surrounded and fix;

(2) Mus fallopian tube or cornua uteri tube wall is broken with acupuncture;

(3) oppress Mouse Oviductal or cornua uteri from the top down, make free shape Mouse Embryos puncture position from Mouse Oviductal or cornua uteri and overflow, then collect embryo.