CN104720070A - Microemulsion pre-concentrated material, microemulsion, and preparation method of microemulsion pre-concentrated material and microemulsion - Google Patents
Microemulsion pre-concentrated material, microemulsion, and preparation method of microemulsion pre-concentrated material and microemulsion Download PDFInfo
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Abstract
The invention discloses a microemulsified system composed of coconut oil and polysorbate 80 at a certain weight ratio. The invention also discloses a microemulsion pre-concentrated material and a microemulsion which contain a lipophilic functional component prepared from the microemulsified system. The invention discloses a preparation method of the microemulsion pre-concentrated material and the microemulsion.
Description
Technical field
The invention relates to a microemulsion be substantially made up of coconut oil and the polysorbate80 of specified weight ratio.The present invention also have about one with this microemulsion prepare containing the microemulsion preconcentrates of a lipophilicity functional composition and microemulsion.The present invention also has the method for making about this microemulsion preconcentrates and microemulsion.This microemulsion can be used for exploitation and the production of new health beverages.
Background technology
In recent years, many functional compositions (functional ingredients), such as, lutein (xanthophyll) in carotenoid (carotenoids), astacin (astaxanthin) and Co-Q10 (co-enzymeQ10, CoQ10), health-care effect (healthcare effects) is found to have.But the water-soluble extreme difference of these functional compositions, which has limited the exploitation of the health product containing these functional compositions.
Along with people are for the attention of health and the requirement for the local flavor (flavor) of health product (healthcareproducts), outward appearance (appearance) and convenience (convenience), the exploitation of health product should not be confined to the pattern of capsule (capsules), ingot sheet (tablets) or the powder (powder) in the past used.Micro-emulsion technology (microemulsion technique) effectively can increase the solubility of fat-soluble compound (liposolublecompounds) Yu Shuizhong.If interfacial agent (surfactants) can be overcome and help the use of interfacial agent (cosurfactants) to limit (use restriction), can make an addition in drink (drinks) by the fat-soluble function composition (microemulsified liposolublefunctional ingredients) through microemulsified, this should contribute to developing new health beverages.
Co-Q10 is a material being present in human body, and it belongs to lipid fractions (liposoluble antioxidants), and can reduce along with the increase at age.Co-Q10 is a prescription medicine (prescription drug) being used in cardiac disease treatment in early days, becomes an emerging health nutrient composition (emerging ingredient for health andnutrition) in recent years due to the oxidation resistance (anti-oxidation ability) of its excellence.In addition, because utilization rate in the body of liposoluble substance (in vivoavailability) is on the low side, the bioavailability (bioavailability) how improving Co-Q10 is absorbed in current research mostly.
The manufacture of Co-Q10 can be divided into fermentable to produce (microbialfermentation) and chemical synthesis (chemical synthesis) these two kinds, and only has Yao Hua pharmaceuticals (No. 3, garden street, Nangang District, Taipei City, Taiwan Province, China F 13rd floors) with chemical synthesis to carry out a large amount of productions of Co-Q10 at TaiWan, China at present.On the other hand, have the water-soluble powder peddling Co-Q10 at present on the market, the Co-Q10 concentration contained in it can reach 40%, but its product price is high far beyond Co-Q10 raw material, and need from external import.When developing new health product, if dealer is too high for the degree of dependence of foreign technology and imported raw material, can improve production cost far and away, with the angle of long-range operation, being just a makeshift arrangement, therefore dealer must find suitable technology or raw material replaces.If a kind of liquid formulation (liquid formulation) can be developed, dealer can be used Co-Q10 raw material to start and manufactures health beverages, the product competitiveness (productcompetitiveness) promoting dealer will be contributed to.
For asking the oral bioavailability rate (oral bioavailability) improving Co-Q10, the people such as P.Thanatuksorn study 5 kinds of greases (fats), 4 kinds of emulsifying agents (emulsifiers) and two kinds of aqueous phases [containing or do not contain the deionized water (distilled water) of 8g/100gw/w skimmed milk (skim milk)], and find to utilize coconut oil (coconut oil), the skimmed milk aqueous solution (skim milk aqueous solution) and stearyl-2-calcium lactate (calcium stearoyl-2-lactate, CSL) emulsification (emulsion) generates an optimum formula.Experimentally result, the people such as P.Thanatuksorn prepare one to carry out emulsification model Co-Q10 product (model CoQ10product) with coconut oil, the 8g/100gw/w skimmed milk aqueous solution and CSL, and wherein the Co-Q10 product through emulsification of 100g is made up of following: the coconut oil of CSL, 2.6g of CoQ10,0.8g of 0.28g, the sugar of 14g, the skimmed milk of 6.8g and the water of 75.52g.The size of the oil droplet (oil droplets) formed immediately after emulsification (emulsification) is 2.37 ± 0.59 μm, and described oil droplet be stored in last 10 days at 4 DEG C after have part to change into be a creamy phase (cream-like phase).The oral bioavailability rate of this model Co-Q10 product is proved than a normal business Co-Q10 product (standard commercial CoQ10 product) (HJB CoenzymeQ10 EX; Fujitex Co., Ltd., Japan) institute tool person slightly higher (P.Thanatuksorn et al. (2009), LWT-Food Science and Technology, 42:385-390).In view of this, the model Co-Q10 product prepared by the people such as Thanatuksorn has a larger particle diameter (particle size), and pot-life (storage time) at 4 DEG C is also permanent not.
The people such as Junya Hatanaka use the cosolvent (solubilizing agents) being generally used as food ingredients to prepare the new formula of Co-Q10, comprise a kind of liquid formulations (liquid formulation) and water-soluble power formulations (water-solublepowder formulation).This liquid formulations is a nanoemulsions (nano-emulsion comprising following composition, NE): in total amount 100%, the Co-Q10 of 10.5%, the non-ionic surfactant (nonionic surfactants) of 12.0%, the soybean lecithin (soybean lecithin) of 3%, the glycerine (glycerol) of 55%, the deionized water of 15.0% and middle long-chain three (acid) glyceride (the medium chain triglycerides of 4.5%, MCT), wherein non-ionic surfactant and soybean lecithin are as an emulsifying agent (emulsifier), and MCT is as an oil phase (Junya Hatanaka et al. (2008), InternationalJournal of Pharmaceutics, 363:112-117).Through looking into, the composition of this non-ionic surfactant and MCT is not quite clear.Need when preparing this nanoemulsions " CoQ10-NE " to use the glycerine of a large amount and two kinds of different components as emulsifying agent, and need to use homogeneous mixer (homomixer) and high pressure homogenizer (high pressure homogenizer) to reach the complete emulsification of formula, this perhaps contributes to CoQ10-NE and has the average grain diameter that is 60nm, but makes the processing procedure of CoQ10-NE complicated.
US20060073176A1 (corresponding to TWI321989B) discloses the water-soluble composition that contains Co-Q10, it contains: (A) 5 ~ 40 Co-Q10 of quality %, (B) 5 ~ 30 quality % by the monoesters (monoester) there is the polyglycereol (polyglycerol with averagedpolymerization degree of 10) that average degree of polymerization is 10 and the aliphatic acid (fattyacid having 18 carbon atoms) with 18 carbon atoms forming, (C) 1 ~ 18 quality % by have list that the polyglycereol that average degree of polymerization is 3 ~ 6 and the aliphatic acid with 18 carbon atoms form-, two-, three-or five-ester (mono-, di-, tri-orpenta-ester), and (D) water.Should be declared to have the average grain diameter (average particle diameter) that is 110nm or less by water-soluble composition containing Co-Q10.Although mention in the disclosure of US 20060073176 A1, the solvent of such as oil is not needed to dissolve or disperse the Co-Q10 of high concentration when preparing this and containing the water-soluble composition of Co-Q10, this case not only needs use two kinds of interfacial agents to make the water-soluble composition that this contains Co-Q10, also must use the 5th kind of composition in certain embodiments, namely (E) cosolvent (solubilizer), comprise containing Glucose-Fructose liquid sugar (glucose-fructoseliquid sugar), Arabic gum (gum arabic), reduction starch sugar (reducing starchsugar), even also to use palm oil (palm oil) or sucrose acetate/isobutyrate (sucrose acetic acid/isobutyric acid ester in certain embodiments, SAIB) come to form an oil phase (oil phase) with Co-Q10 as an oily component (oil component).In addition, contain in the manufacture process of the water-soluble composition of Co-Q10 need to use homogeneous mixer and high pressure homogenizer to reach the complete emulsification of formula at this, which increase production equipment demand.
US 20070259034 A1 discloses a kind of not containing the Co-Q10 composition (crystal-free coenzymeQ10composition) of crystallization, and it comprises Co-Q10, a solvent and a carrier oil (carrier oil).According to US 20070259034 A1, this solvent can be CLA (conjugated linoleic acid, CLA), Linseed oil (flaxseed oil), marine organisms lipid ethyl ester (ethyl ester marine lipids), mandarin oil (citrus oils) or their combination.According to US 20070259034 A1, this carrier oil can be Linseed oil, comprise linolenic acid organic Linseed oil of (alpha linoleic acid, ALA), soybean lipids (soy lipids), Common Borage lipid (borage lipids) or marine organisms lipid.Marine organisms lipid is the concentrate of EPA/DHA ethyl ester (ethyl ester EPA/DHA) and can be the EPA/DHA (EPA/DHA combined) of 50% to 90% compound.According to US 20070259034 A1, this Co-Q10 composition comprises capric acid and caprylin (capric and caprylic glycerides) further, or vegetable oil monoglyceride (vegetable monoglycerides), or the mixture that is made up of vegetable oil monoglyceride and two glyceride (a mixture of vegetable monoglycerides anddiglycerides).According to US 20070259034 A1, this Co-Q10 composition will be packaged in soft capsule (softgel) for oral (oral administration).In view of this, the Co-Q10 composition that US 20070259034 A1 discloses is not suitable for the health product, particularly health beverages that manufacture in liquid form.
US 20080145411 A1 discloses the oral composition of a kind of confession, it includes a CoQ10 (oxidized coenzymeQ10), a lysolecithin (lysolecithin) and an oil and fat (oil and fat), and wherein lysolecithin is be not less than 0.7 relative to the weight ratio (weight ratio) of CoQ10.Lysolecithin is a higher-priced chemicals, and its use can make manufacturing cost increase.In addition, it seems from whole embodiments of US 20080145411 A1, the oral composition of this confession is not suitable for the health product, particularly health beverages that manufacture in liquid form.
US 20080248013 A1 (corresponding to TW I351925 B) discloses a kind of fluid composition containing Co-Q10, it obtains in the waterborne liquid (aqueous liquid) that contains glycerine and a water-soluble substances (water-solublesubstance) by by loose for Co-Q10 floating (dispersing) and emulsification (emulsifying), and this water-soluble substances is made up of octenyl succinic acid starch (octenylsuccinate starch) and dextrin (dextrin).This fluid composition can be dried and form one containing the solid composite of Co-Q10.Through looking into, high pressure homogenizer must be used to reach the complete emulsification of formula when preparing this fluid composition, which increasing production equipment demand.Moreover fluid composition manufactured in the embodiment of US 20080248013 A1 has the particle diameter that is not less than 0.3 μm, and this cannot meet the outward appearance demand of drinking production.
US 20040152612 A1 discloses the microemulsion preconcentrates (microemulsion preconcentrates) and microemulsion that contain Co-Q10.This microemulsion preconcentrates includes a mixture be made up of long-chain three (acid) glyceride in one and ω-9 aliphatic acid and/or ω-6 aliphatic acid following formed mixture: (a); (b) surface-active component (surface-active component), it includes the interfacial agent that is polyoxyethylene pattern (polyoxyethylene type); And (c) is from the therapeutic activity composition (therapeutic activeingredient) of ubiquinone family (ubiquinone class), particularly Co-Q10.Prepared 3 microemulsion preconcentrates in the embodiment 1 of US 20040152612 A1, wherein component (a) is
812 (a1) and oleic acid (oleicacid) (a2) (it is a kind of emulsifying agent or cosolvent), component (b) is Tween80 (b), component (c) is Co-Q10 (c1), and be added with vitamin E (c2) or Emulsifier EL-60 (CremophorEL) (b2) (it is a kind of cosolvent) in addition, and the weight ratio of component (b) and component (a) is about 1:1 or 9:7.
US 20040126367 A1 (corresponding to TW I334862 B) discloses a kind of solution containing reduced coenzyme Q 10 (reduced coenzymeQ10), wherein reduced coenzyme Q 10 is wrapped by with liposome (liposome) or it is dissolved or emulsification with interfacial agent, avoids oxidation to maintain reduced coenzyme Q 10.Material for the formation of this liposome can be Phospholipids (phospholipid) or glycolipid matter (glycolipid).Interfacial agent is preferably non-ionic surfactant, is more preferred from the polyoxyethylene hardened castor oil (polyoxyethylene hardened castor oil) of poly-D-sorbite system interfacial agent (polysorbate surfactant) of such as Tween80 and so on or such as HCO60 and so on.In the embodiment 1 of US 20040126367 A1, assess the oxidation stability of liposome for reduced coenzyme Q 10 with 3 kinds of lecithin.In the embodiment 2 of US 20040126367 A1, assess the oxidation stability of interfacial agent for reduced coenzyme Q 10 with Tween 80 and HCO60 (aqueous solution of 1wt% or 0.1wt%).The emulsion that the preparation examples 2 (preparationexample 2) of US 20040126367 A1 provides one to comprise following composition: Tween 80,1.0wt%; Glycerine, 12.5wt%; Phosphatid ylcholine (phosphatidyl choline), 1.2wt%; Reduced coenzyme Q 10,0.1wt%; Purified water (purified water), is added to 100.0wt%.
US 20100284983 A1 (corresponding to TW 200715995 A) discloses a kind of water-soluble composition (water soluble composition) containing Co-Q10, and it contains Co-Q10, a hydrophily polyglyceryl fatty acid ester (hydrophilic polyglycerol fatty acidester), a lipophile sucrose fatty ester (lipophilic sucrose fatty acidester) and an aqueous phase component (aqueous phase component).This hydrophily polyglyceryl fatty acid ester is the ten glycerine list polyunsaturated fatty acid esters (decaglycerol mono-saturated fatty acidester) be made up of a fatty acid residue containing 12 or more carbon atoms, is more preferred from least one be selected from group that laurate (laurate ester), myristinate (myristate ester) and palmitate (palmitate ester) form.This lipophile sucrose fatty ester is preferably an ester be made up of higher fatty acids (higher fatty acid) and acetic acid (acetic acid), is more preferred from sucrose palmitate, sucrose stearate and their acetylate (acetylationproduct).Relative to the Co-Q10 of 100 weight portions (parts by weight), the addition of this lipophile sucrose fatty ester is preferably 1 ~ 200 weight portion, be more preferred from 5 ~ 150 weight portions, be more preferred from again 10 ~ 100 weight portions, special good is 30 ~ 100 weight portions.This aqueous phase component contains a polyalcohol (polyhydric alcohol) and/or water.This polyalcohol is preferably glycerine and D-sorbite (sorbitol).Through looking into, when manufacturing this and containing the water-soluble composition of Co-Q10, must carry out heating to dissolve used component in specified temp (50 ~ 70 DEG C), and need to use high pressure homogenizer to carry out emulsification treatment.
US 20060051462 A1 discloses the self-emulsification composition (self emulsifyingcompositions) for transporting lipophilicity Co-Q10 and other meals compositions (dietary ingredients).Through looking into, oily composition of more than two kinds and/or emulsifying agent of more than two kinds are used in the solution formula (solutionformulation) illustrated in embodiment of US 20060051462 A1, and this can make manufacturing cost increase far and away.Moreover the character of these solution formulas that US 20060051462A1 discloses is not quite clear.
Many liquid nanometer emulsion concentrates (liquid nanoemulsion concentrates) are illustrated in the embodiment of US 20090317532 A1, their formula needs to use at least a kind of oily composition, at least a kind of emulsification stabilization agent (emulsion stabilizer), at least a kind of interfacial agent, even need interpolation to help interfacial agent, this can make manufacturing cost increase far and away.Moreover the preparation of oil phase and aqueous phase must be carried out heating to make used component dissolve each other at 60 DEG C, and need to use high pressure homogenizer to carry out emulsification treatment.
Summary of the invention
So, in the 1st, the invention provides a kind of microemulsion preconcentrates (microemulsion preconcentrate) containing a lipophilicity functional composition, this microemulsion preconcentrates forms an oil-in-water type (oil-in-water when diluting with an aqueous medium (aqueous medium), o/w) microemulsion, and this microemulsion preconcentrates is be made up of (consist essentially of) following substantially:
One microemulsion (microemulsifying system) be made up of coconut oil and polysorbate80 (Polysorbate 80), wherein coconut oil is 1:4 to 1:9 relative to the weight ratio (weight ratio) of polysorbate80, and
One is dissolved in the lipophilicity functional composition in this microemulsion, and wherein this lipophilicity functional composition is be not more than 10 relative to the weight ratio of this microemulsion.
In the 2nd, the invention provides the oral oil-in-water microemulsion of a kind of confession (o/wmicroemulsion for oral administration), it is by diluting a microemulsion preconcentrates as above with an aqueous medium and obtained.
In the 3rd, the invention provides a kind of health beverages, it contains an oil-in-water microemulsion as above.
In the 4th, the invention provides a kind of method for the preparation of a microemulsion preconcentrates as above, it comprises:
Carry out Homogeneous phase mixing one lipophilicity functional composition with coconut oil, Homogeneous phase mixing is with polysorbate80 then,
Wherein:
Coconut oil is 1:4 to 1:9 relative to the weight ratio of polysorbate80, and
This lipophilicity functional composition is be not more than 10 relative to the weight ratio of the summation (sum) of coconut oil and polysorbate80.
In the 5th, the invention provides a kind of method for the preparation of an oil-in-water microemulsion as above, it comprises:
Carry out Homogeneous phase mixing one lipophilicity functional composition with coconut oil, Homogeneous phase mixing is with polysorbate80 then, and a microemulsion preconcentrates is formed, and
This microemulsion preconcentrates is diluted with an aqueous medium,
Wherein:
Coconut oil is 1:4 to 1:9 relative to the weight ratio of polysorbate80, and
This lipophilicity functional composition is be not more than 10 relative to the weight ratio of the summation of coconut oil and polysorbate80.
Beneficial effect of the present invention is: when carrying out mixed coconut oil with polysorbate80 with a specific weight ratio, boundary/the oil mixture formed can form a stable oil-in-water microemulsion with an aqueous medium, or prepare stable microemulsion preconcentrates with the lipophilicity functional composition of such as Co-Q10, can add any help interfacial agent (such as the art belong in the middle of usual short chain acids, alcohol or aliphatic acid etc.), also high pressure homogenizer need not be used to carry out emulsification, the lipophilicity functional uniform ingredients of such as Co-Q10 can be made at normal temperatures to be scattered in an aqueous medium and to form a stable microemulsion, have more easy and simple to handle, the advantage reduced costs.
Accompanying drawing explanation
Fig. 1 display using Tween80 as interfacial agent, coconut oil is as oil phase and deionized water as aqueous phase, be 9/1 (left in boundary/oil ratio, T91) or 8/2 (right, T82), under, deionized water is added to gross weight for 5g and obtained test specimen can form the oil-in-water microemulsion (clear and transparent o/w microemulsion) of transparent clarification after Homogeneous phase mixing with 0.5,1.0 or the boundary/oil mixture of 1.5g respectively;
Fig. 2 is the three-phase diagram of the microemulsion that is set up according to the present invention, the oil phase being wherein used for setting up this microemulsion is coconut oil, interfacial agent be Tween 80 and aqueous phase is deionized water, and hatched example areas (hatched area) is micro-emulsion region (microemulsion region);
The pH value change that Fig. 3 shows aqueous phase there is no obvious impact for the microemulsion set up according to the present invention, the oil phase being wherein used for setting up described microemulsion is coconut oil, interfacial agent is Tween 80, and aqueous phase is respectively the phosphate buffer of pH2.0, pH value is respectively the 0.1M citric acid-sodium citrate buffer solution of 3,4,5 or 6, or the phosphate buffer of pH8.0; And hatched example areas is micro-emulsion region;
Fig. 4 shows one according to the stability in storage of microemulsion of the present invention under different temperatures (room temperature, 37 DEG C with 55 DEG C), the oil phase being wherein used for setting up this microemulsion is coconut oil, interfacial agent is Tween80, and aqueous phase is deionized water; T91-10X represents boundary/oil ratio=9/1, and extension rate is 10 times; T91-50X represents boundary/oil ratio=9/1, and extension rate is 50 times; T91-100X represents boundary/oil ratio=9/1, and extension rate is 100 times; T82-10X represents oil ratio=8/2, boundary, and extension rate is 10 times; T82-50X represents boundary/oil ratio=8/2, and extension rate is 50 times; And T82-100X represents boundary/oil ratio=8/2, extension rate is 100 times;
The oil-in-water microemulsion containing Co-Q10 that Fig. 5 shows use microemulsion of the present invention and generates, the liquid wherein in the sample bottle of right is the product obtained by embodiment 2, and the liquid in the sample bottle of left is 10 times of dilution product of this product;
Fig. 6 show two kinds of microemulsion samples obtained by embodiment 3 before UHTS process with after outward appearance, the aqueous phase being wherein used to form the microemulsion samples in the picture of left is deionized water; And the aqueous phase of the microemulsion samples be used to form in the picture of right is commercially available green tea;
The Co-Q10 concentration (ppm) of rat in the blood plasma measured by different time points that Fig. 7 display is filled a prescription with the formula of the present invention containing Co-Q10 or comparative example by feeding;
The oil-in-water microemulsion containing curcumin that Fig. 8 shows use microemulsion of the present invention and generates; And
The oil-in-water microemulsion containing beta carotene that Fig. 9 shows use microemulsion of the present invention and generates.
Detailed description of the invention
The microemulsion composition being applicable to health beverages for providing one, applicant finds through experiment, when carrying out mixed coconut oil with a specific weight ratio with polysorbate80, the boundary/oil mixture formed can form a stable oil-in-water microemulsion with an aqueous medium.Applicant finds further, and the oil-in-water microemulsion containing a lipophilicity functional composition that the microemulsion formed with coconut oil and polysorbate80 is prepared has the particle diameter that is not more than 100nm, and presents the outward appearance of transparent clarification.In addition, in the middle of the process of this oil-in-water microemulsion of preparation, emulsion process can be carried out at normal temperatures and pressures, and does not need to use high pressure homogenizer.This cover micro-emulsion technology that applicant develops in the present invention has advantage that is easy and simple to handle, that reduce costs, is very suitable for exploitation and the production of the health beverages containing lipophilicity function composition.
Therefore, the invention provides a kind of microemulsion preconcentrates containing a lipophilicity functional composition, this microemulsion preconcentrates forms an oil-in-water microemulsion when diluting with an aqueous medium, and this microemulsion preconcentrates is be made up of following substantially:
One microemulsion be made up of coconut oil and polysorbate80, wherein coconut oil is 1:4 to 1:9 relative to the weight ratio of polysorbate80, and
One is dissolved in the lipophilicity functional composition in this microemulsion, and wherein this lipophilicity functional composition is be not more than 10 relative to the weight ratio of this microemulsion.
The present invention also provides a kind of method for the preparation of a microemulsion preconcentrates as above, and it comprises:
Carry out Homogeneous phase mixing one lipophilicity functional composition with coconut oil, Homogeneous phase mixing is with polysorbate80 then,
Wherein:
Coconut oil is 1:4 to 1:9 relative to the weight ratio of polysorbate80, and
This lipophilicity functional composition is be not more than 10 relative to the weight ratio of the summation of coconut oil and polysorbate80.
Coconut oil is the ripe coconut palm really pulp (meat) of (matured coconut) or the edible oil (edible oil) of kernel (kernel) of a kind of extraction from coco (coconut palm, formal name used at school is Cocosnucifera L.).Coconut main body of oil is: 44.6% laurate (lauric acid), 16.8% myristic acid (myristic acid), 8.2% palmitic acid (palmitic acid), 8% sad (caprylic acid) and 6% oleic acid.
Polysorbate80 is a kind of non-ionic surfactant derived from polyoxyethylated sorbitol acid anhydride (polyethoxylated sorbitan) and oleic acid and emulsifying agent.The brand name (brand name) of polysorbate80 comprises Tween, Alkest and Canarcel etc.
As used herein, term " lipophilic substance ", " lipophilic compound " or analog mean one and not to be soluble in an aqueous solution but the material that can mix mutually with a lipid.In the present invention, term " lipophilicity " and " oil-soluble (oil-soluble) " can exchange use.
As used herein, term " functional composition " comprises and is intended to for the treatment of disease (disease) or uncomfortable (illness), prevention, diagnosis, the physiology curing or alleviate (mitigation) or pharmacological active substance, or for can provide the material of nutrition to a certain degree or treatment benefit to this animal when being given an animal.
According to the present invention, this lipophilicity functional composition comprises, but be not limited to: Co-Q10 (comprising CoQ10, reduced coenzyme Q 10 etc.), curcumin, carotenoid are (such as, beta carotene, lutein), astacin, Lycopene, resveratrol, DHA etc., or their combination.
In a preferred embodiment of the present invention, this lipophilicity functional composition is Co-Q10.In another preferred embodiment of the present invention, this lipophilicity functional composition is curcumin.In another preferred embodiment of the present invention, this lipophilicity functional composition is beta carotene.
As used herein, term " aqueous medium " means one to include the water of substantial amount liquid medium according to the unit volume of liquid medium.Be applicable to aqueous medium of the present invention comprise, but be not limited to, those are applicable to the aqueous medium manufacturing commercial beverage or health beverages, such as pure water, deionized water, mineral water, fruit juice, vegetable juice, carbonated drink, cola, sarsaparilla, cow's milk, Yoghourt, soya-bean milk, beverage tea, sports drink etc.
In a preferred embodiment of the present invention, this aqueous medium is deionized water.In another preferred embodiment of the present invention, this aqueous medium is green tea beverage (green teadrink).
In a preferred embodiment of the present invention, coconut oil is 1:4 relative to the weight ratio of polysorbate80.In another preferred embodiment of the present invention, coconut oil is 1:9 relative to the weight ratio of polysorbate80.
According to the present invention, this lipophilicity functional composition preferably drops in the scope of 1:10000 to 1:10 relative to the weight ratio of this microemulsion, be more preferred from and drop in the scope of 1:1000 to 1:10, be more preferred from again and drop in the scope of 1:250 to 1:10, be more preferred from again and drop in the scope of 1:150 to 1:10.In a preferred embodiment of the present invention, this lipophilicity functional composition is 1:30 relative to the weight ratio of this microemulsion.In another preferred embodiment of the present invention, this lipophilicity functional composition is 1:60 relative to the weight ratio of this microemulsion.In another preferred embodiment of the present invention, this lipophilicity functional composition is 1:250 relative to the weight ratio of this microemulsion.
In a preferred embodiment of the present invention, this lipophilicity functional composition is Co-Q10, and Co-Q10 is 1:30 relative to the weight ratio of this microemulsion.
In another preferred embodiment of the present invention, this lipophilicity functional composition is curcumin, and curcumin is 1:60 relative to the weight ratio of this microemulsion.
In another preferred embodiment of the present invention, this lipophilicity functional composition is beta carotene, and beta carotene is 1:250 relative to the weight ratio of this microemulsion.
The oil-in-water microemulsion that the present invention also provides a kind of confession oral, it is by diluting a microemulsion preconcentrates as above with an aqueous medium and obtained.
The present invention is also provided for the method for preparation one oil-in-water microemulsion as above, and it comprises:
Carry out Homogeneous phase mixing one lipophilicity functional composition with coconut oil, Homogeneous phase mixing is with polysorbate80 then, and a microemulsion preconcentrates is formed, and
This microemulsion preconcentrates is diluted with an aqueous medium,
Wherein:
Coconut oil is 1:4 to 1:9 relative to the weight ratio of polysorbate80, and
This lipophilicity functional composition is be not more than 10 relative to the weight ratio of the summation of coconut oil and polysorbate80.
The aqueous medium being applicable to preparation method of the present invention include, but are not limited to those in one depicted above.
According to the present invention, the aqueous medium of a minimum quantity can be used to dilute this microemulsion preconcentrates, and the oil-in-water microemulsion formed can be diluted with more substantial aqueous medium and do not affected the particle diameter of the nano particle be present in microemulsion further.According to the present invention, the minimum quantity of this aqueous medium is about 2 times of the weight of this microemulsion preconcentrates.
According to the present invention, this aqueous medium has the pH value that is 2 ~ 8, is preferably and has the pH value that is 5 ~ 8.In a preferred embodiment of the present invention, the pH value of this aqueous medium be oral beverage can pH value, such as pH value is 7.
When preparation method of the present invention uses one to be applicable to the aqueous medium manufacturing commercial beverage or health beverages, the oil-in-water microemulsion formed can be manufactured into health beverages.
Preparation method of the present invention can carry out at normal temperatures and pressures, and does not need to use high pressure homogenizer can reach the Homogeneous phase mixing of all components.
Embodiment
The present invention will be described further with regard to following examples, but it is to be understood that described embodiment just illustratively illustrates use, and should not be interpreted as the restriction in enforcement of the present invention.
Experiment material:
Except as otherwise noted, the water used in embodiment is below all deionized water (de-ionized water).
Llowing group of materials is purchased from Sigma-Aldrich Co.LLC., USA: coconut oil (coconut oil), natrium citricum (sodium citrate tribasic dehydrate), beta carotene (β-carotene) and sanlose (sodiumcarboxymethyl cellulose, CMC).
1% (w/v) CMC solution used in the middle of embodiment below prepares with deionized water.
Llowing group of materials is purchased from TaiWan, China Merck S. A. No. 89, Di Ding Road No. 2 section, Nei Hu District, Taibei city, Taiwan, China (6th floors): Tween60, Tween80, methyl alcohol (methanol), ethanol (ethanol), hexane (hexane), isopropyl ether (isopropylether), phosphoric acid, sodium dihydrogen phosphate and sodium hydrogen phosphate.
Citric acid (citric acid) is purchased from Zhen Fang limited company (Taibei city, Taiwan Province, P.R.china newborn No. 116 Zhen Fang buildings, one section, South Road).
Except as otherwise noted, the 0.1M citric acid-sodium citrate buffer solution (citric acid-sodium citrate buffer) with pH3.0 ~ 6.0 used in embodiment be below according to for preparation pH value, respectively a certain proportion of citric acid and natrium citricum to be added in deionized water and to mix, finally doing to confirm with pH meter (pH meter).
The phosphate buffer (phosphoric acid buffer) with pH2.0 be according to for the pH value of preparation, a certain proportion of phosphoric acid and sodium dihydrogen phosphate to be added in deionized water and to mix, finally doing to confirm with pH meter.
The phosphate buffer (phosphate buffer) with pH8.0 be according to for the pH value of preparation, a certain proportion of sodium dihydrogen phosphate and sodium hydrogen phosphate to be added in deionized water and to mix, finally doing to confirm with pH meter.
Oil soluble coenzymes Q10 (marque:
) be purchased from Pharmaessentia Corp. (No. 3, garden street, Nangang District, Taipei City, Taiwan Province, China F 13rd floors).
Curcumin (curcumin) is purchased from Deng Sheng enterprise stock Co., Ltd No. 189, Chien road, science park port, inland lake, Taibei city, Taiwan Province, P.R.china (9th floors).
Embodiment 1. uses coconut oil as oil phase to set up microemulsion of the present invention (Establishment of microemulsifying systems of thisinvention using coconut oil as oil phase)
For setting up according to microemulsion of the present invention, the present embodiment uses coconut oil as oil phase (oil phase), and inquire into the kind of interfacial agent (surfactant) and the pH value (the aqueous phase ' s pH value) of aqueous phase form (the formationof microemulsion) impact for microemulsion, also has the impact of the stability in storage (storage stability) inquiring into the microemulsion that temperature is set up for the present invention, and analyze viscosity (viscosity) and the particle diameter (particle size) of the microemulsion that the present invention sets up.
A. interfacial agent is for the impact (The influenceof surfactant upon the microemulsifying system of thisinvention) according to microemulsion of the present invention
This experiment uses coconut oil as oil phase, Tween60 or Tween80 is as interfacial agent, and deionized water is as aqueous phase, to find out the component and their usage ratio scope that can form microemulsion.
Experimental technique:
By coconut oil and a selected interfacial agent with a boundary/oil ratio of specifying (surfactant/oil ratio) (9/1,8/2,7/3,6/4,5/5,4/6,3/7,2/8 or 1/9, w/w, gross weight is 50g) be added in a container, and utilize magnetite agitator (Corning PC-420D) evenly to mix boundary/oil mixture (surfactant/oil mixture) in this container with the stir speed (S.S.) that is about 800rpm.
For each through mixed uniformly boundary/oil mixture, take out a quantity (0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0 or 4.5g) of specifying respectively and be incorporated in sample bottle (empty sample bottle), then adding deionized water until the gross weight of test specimen (test sample) reaches 5g in sample bottle.Utilize and be same as above-mentioned magnetite agitating mode to the test specimen stirred in each sample bottle and observe its cosmetic variation, namely whether have formation microemulsion.The result forming microemulsion whether is had to draw three-phase diagram (ternary phase diagram) relative to the dilution ratio (dilution ratio) of aqueous phase and test specimen according to the boundary/oil ratio of coconut oil and interfacial agent, boundary/oil mixture.
Experimental result:
Applicant finds: when using coconut oil as oil phase and using Tween60 as interfacial agent, and the boundary/oil mixture formed under each boundary/oil ratio all cannot come with aqueous phase Homogeneous phase mixing with different extension rates to form the microemulsion of transparent clarification.Relatively, when using coconut oil as oil phase and using Tween80 as interfacial agent, as shown in Figure 1, boundary/oil ratio for 9/1 or boundary/oil mixture of being formed for 8/2 time can form the microemulsion of transparent clarification with aqueous phase Homogeneous phase mixing.
Whether applicant can form the result of microemulsion with aqueous phase according to coconut oil and Tween80 and draw out a three-phase diagram as shown in Figure 2 under each boundary/oil ratio and different dilution ratio, wherein have region that microemulsion formed be between initial boundary/oil ratio be 8/2 and 9/1 these two hatched example areas (hatched area) of diluting between line.
Applicant finds again: when boundary/oil ratio is 8/2, and the water content (watercontent) of test specimen need be close to 70% (w/w) or more and just can form microemulsion; And boundary/oil ratio is when being increased to 9/1, the water content of test specimen can form microemulsion at 60% (w/w) or more.Reach minimum limitation just can form microemulsion as long as have by the test specimen water content that boundary/oil mixture that this Liang Ge circle/oil ratio is formed is prepared, and the microemulsion formed can also dilute with a large amount of water and not affect the nano particle be present in microemulsion.
B. the pH value of aqueous phase is for the impact (The influenceof the aqueous phase ' s pH value upon the microemulsifyingsystem of this invention) according to microemulsion of the present invention
For finding out coconut oil and Tween80 can form microemulsion within the scope of how wide aqueous phase pH, this experiment uses the following cushioning liquid (buffered solution) with different pH value to be used as aqueous phase:
(1) phosphate buffer of pH2.0;
(2) pH value is respectively the 0.1M citric acid-sodium citrate buffer solution of 3,4,5 or 6; And
(3) phosphate buffer of pH8.0.
Experimental technique:
As above-mentioned A joint when described in, prepare the boundary/oil mixture with different boundary/oil ratio (9/1,8/2,7/3,6/4,5/5,4/6,3/7,2/8 or 1/9, w/w) with coconut oil and Tween80.For each through mixed uniformly boundary/oil mixture, take out a quantity (0.5,1.0,1.5,2.0,2.5,3.0,3.5,4.0 or 4.5g) of specifying respectively and be incorporated in sample bottle, then adding a selected cushioning liquid until the gross weight of test specimen reaches 5g in sample bottle.Utilize and be same as magnetite agitating mode described in the joint of A above to the test specimen stirred in each sample bottle and observe its cosmetic variation, namely whether have formation microemulsion.The result forming microemulsion whether is had to draw three-phase diagram relative to the dilution ratio of aqueous phase and test specimen according to the boundary/oil ratio of coconut oil and Tween80, boundary/oil mixture.Experimental result:
Whether applicant can form the result of microemulsion with selected cushioning liquid and draw out a three-phase diagram as shown in Figure 3 according to coconut oil and Tween80 under each boundary/oil ratio and different dilution ratio.As shown in Figure 3, when the pH of aqueous phase is reduced to below 4, micro-emulsion region (microemulsion region) has and slightly reduces, but generally speaking, the pH value change of aqueous phase there is no obvious impact for the foundation according to microemulsion of the present invention.
C. temperature is for the impact (Theinfluence of temperature upon the storage stability of themicroemulsifying system of this invention) of the stability in storage according to microemulsion of the present invention
Which kind of impact the change of this experiment discussion temperature can produce to the stability in storage according to microemulsion of the present invention.
Experimental technique:
As described in A joint is worked as above, with the boundary/oil ratio of 9/1 or 8/2 (w/w), Tween80 and coconut oil are evenly mixed and obtain one boundary/oil mixture.One appropriate boundary/oil mixture has been placed in sample bottle, and with deionized water give dilution 10 times, 50 times or 100 times, then utilize and be same as described magnetite agitating mode in the joint of A above and carry out the test specimen in stirred sample bottle until there is microemulsion to be formed.Afterwards, sample bottle is stored in room temperature, at 37 DEG C or 55 DEG C.Microemulsion in sample bottle is sampled at different time points (the 0th, 1,5,7,12,16,21 and 28 day), and carries out light transmittance analysis (optical transmittance analysis) with a point luminometer (Merck SpectroquantPharo300) under 600nm.Experimental results is shown in Fig. 4.
Experimental result:
See Fig. 4, last 28 days at the microemulsion that boundary/oil mixture that Tween80 and coconut oil evenly mix with the boundary/oil ratio of 9/1 or 8/2 (w/w) and obtain is formed after diluting 10 times, 50 times or 100 times with deionized water is stored in room temperature or 37 DEG C, boundary/oil ratio is that the light transmittance of the experiment group (test groups) of 9/1 or 8/2 there is no generation significant change.And when storage temperature is increased to 55 DEG C, boundary/oil ratio be 8/2 experiment group can there is white casse along with the growth between the storage life, and the lower experiment group turbidity of dilution ratio is higher.This shows microemulsion of the present invention and have excellent stability in storage at the temperature of room temperature or 37 DEG C, and not affected relative to the dilution ratio of aqueous phase by the boundary/oil ratio of Tween80 and coconut oil and boundary/oil mixture.
D. the viscosity of microemulsion of the present invention and granularmetric analysis (Viscosity and particlesize analyses of the microemulsifying system of thisinvention)
Experimental technique:
As described in A joint is worked as above, with the boundary/oil ratio of 9/1 or 8/2 (w/w), Tween80 and coconut oil are evenly mixed and obtain one boundary/oil mixture.One appropriate boundary/oil mixture has been placed in sample bottle, and has given dilution 10 times with deionized water, then utilize be same as above in A joint described magnetite agitating mode carry out the test specimen in stirred sample bottle until there is microemulsion to be formed.Afterwards, the microemulsion samples in each sample bottle is analyzed with viscosimeter (Nameter viscometer 1810 SF) and particle size analyzer (Beckman 440SX Delsa Laser Particle Measuring Device).
Experimental result:
The water content of two microemulsion samples prepared by this experiment is all 90wt%.See table 1, they are recorded has the viscosity that is about 3.5 ~ 4cP.Compared to the viscosity (1cP) of pure water, the viscosity of these two microemulsion samples there is no obvious increase.About particle diameter, boundary/oil ratio be 9/1 microemulsion samples there is the average grain diameter (average particlesize) that is about 1.9nm, and the microemulsion samples that boundary/oil ratio is 8/2 has the average grain diameter that is about 3.73nm.In view of this, adding more interfacial agent can make the particle diameter of decentralized photo (dispersed phase is namely scattered in the nano particle in microemulsion) diminish.
The viscosity of table 1. microemulsion samples and particle diameter
Embodiment 2. prepares the oil-in-water microemulsion (Preparation ofCoQ10-containing o/w microemulsion) containing Co-Q10
The present embodiment uses one to prepare according to the microemulsion of previous embodiment 1 oil-in-water microemulsion that contains Co-Q10, wherein this microemulsion system is using coconut oil as oil phase, using Tween80 as interfacial agent, and using deionized water as aqueous phase, and Tween80 is 8/2 relative to the boundary/oil ratio of coconut oil.
Experimental technique:
Use a ultrasonic vibrating cleaning machine (Delta Ultrasonic Cleaner DC400), be dissolved in equably in the coconut oil of 6g with the oil soluble coenzymes Q10 of ultrasonic mode of oscillation by 1000mg.Or, Co-Q10 is dispersed in rapidly in oil phase (such as, coconut oil), general magnetite agitator also can be used to reach.
Then utilize magnetite agitating mode to be added in Co-Q10/coconut oil blend by the Tween80 of 24g, after complete Homogeneous phase mixing, add the deionized water of 69g again and stir until exist without colloid substance (gel-like substance) in mixture with magnetite constantly.Obtained product is analyzed with particle size analyzer.Take out 10g from this product, and dilute 10 times with deionized water, then observe cosmetic variation.
Experimental result:
See the sample bottle of Fig. 5 right, the product that the present embodiment obtains is an orange oil-in-water microemulsion containing 10mg/g Co-Q10, and it is measured to has the average grain diameter that is about 60.2nm.Applicant finds, this microemulsion can be diluted to any concentration with an aqueous matrix (aqueous medium), and the average grain diameter of microemulsion not affected by dilution.Such as, namely the yellow liquid in the sample bottle of Fig. 5 left is the oil-in-water microemulsion containing 1mg/g Co-Q10 formed after the product dilution 10 times obtained by the present embodiment by deionized water, and its average grain diameter is about 60.2nm.
Embodiment 3. high-temperature sterilization is for the impact (The influenceof high temperature sterilization upon themicroemulsifying system of this invention) of microemulsion of the present invention
The oil-in-water microemulsion containing Co-Q10 whether the present embodiment discussion high-temperature sterilization can affect use microemulsion of the present invention and generate.
Experimental technique:
According to embodiment 2, use a ultrasonic vibrating cleaning machine (Delta UltrasonicCleaner DC400), be dissolved in equably in the coconut oil of 5g with the oil soluble coenzymes Q10 of ultrasonic mode of oscillation by 250mg.Then utilize magnetite agitating mode to be added in Co-Q10/coconut oil blend by the Tween80 of 20g, the commercially available green tea of the deionized water or 4000mL that add 5000mL after complete Homogeneous phase mixing again also stirs until exist without colloid substance in mixture with magnetite constantly.Take out the product of a sample aliquot (aliquot) (10mL/ bottle), and carrying out UHTS with UHT/HTST sterilization machine (applicant discloses according to the technology of No. 100960th, TaiWan, China new patent the machine assembled), sterilising conditions is 135 DEG C and lasts 3 ~ 5 seconds.
Experimental result:
When preparation of drinks, UHTS (ultra high temperaturesterilization, or flash pasteurization (ultra high temperatureshort time sterilization UHT), HTST) be one of common sterilizing methods, and sterilising conditions is generally the temperature of 130 ~ 145 DEG C and the time of 2 ~ 5 seconds.Fig. 6 show two kinds of microemulsion samples obtained by the present embodiment before carrying out UHTS with after apparent condition, the aqueous phase being wherein used to form the microemulsion samples in the picture of left is deionized water, and the aqueous phase being used to form the microemulsion samples in the picture of right is commercially available green tea.It seems from result shown in Fig. 6, two kinds of microemulsion samples obtained by the present embodiment are not producing obvious cosmetic variation after UHTS, and this display ultrahigh-temperature sterilization can be used to process the oil-in-water microemulsion using microemulsion of the present invention to generate.
Embodiment 4. feeding rats test (Rat feeding test)
The oral absorption rate of the oil-in-water microemulsion type Co-Q10 formula that the present embodiment is inquired into use microemulsion of the present invention and generated.
Experiment material:
A. feeding sample:
The sample of the present invention's formula is one containing the oil-in-water microemulsion of oil soluble coenzymes Q10, and it is prepared with reference to embodiment 2, and contains oil soluble coenzymes Q10,8wt%Tween80,2wt% coconut oil of 10mg/g and surplus is deionized water.
The sample of comparative example formula (comparative formulation) is the 1%CMC solution containing 10mg/mL oil soluble coenzymes Q10.
B. animal used as test:
7 week age, large SLC:SD system male rat (Japan SLC, Inc., Hamamatsu, Japan) was used in this animal experiment after 1 week through raising and train (acclimatization).In the middle of during raising and train, rat is set to have in the Animal House (animal room) of following condition by raising in one: the illumination of the temperature of 20 ~ 26 DEG C, the humidity of 40 ~ 70% and 12 hours/day, and allowed freely edible solid food (CE-2, CLEA Japan, Inc., Tokyo, Japan) and running water.
Experimental technique:
Feeding dosage: every rat gives the single dose that is 60mg Co-Q10/kg body weight, and each formulation samples is respectively tested with 3 rats.
Sampling time: to take a blood sample 0.5mL carry out centrifugal, at then obtained plasma sample being stored in-20 DEG C from arteria caudalis for 0.5,1,2,4,8,12,24 hour before feeding with after feeding.
Extraction mode: by (thawed) plasma sample (200 μ L) of thawing and 200 μ L ethanol Homogeneous phase mixing, connect and with 200 μ L hexane Homogeneous phase mixing (ScientificIndustries Vortex-Genie 2); After shaking 5 minutes through ultrasonic (DeltaUltrasonic Cleaner DC400), by centrifugal for mixture (Heraeas Instruments Biofuge fresco, 12,000rpm, 10 minutes), then get organic layer to carry out drying under reduced pressure (
-215); Obtained desciccate back dissolving is used high-performance liquid chromatograph (Agilent, Series1200) to carry out HPLC analysis in the 300 μ L ethanol.
HPLC condition:
1. tubing string: MerckC-18 tubing string (250X4mm, 5 μm)
2. mobile phase: methyl alcohol/isopropyl ether (85:15), punching such as degree of grade is carried (isocratic elution)
3. analytical wavelengths: 275nm
4. condition is put forward in punching: 1mL/min
5. sample volume: 50 μ L
6. tubing string temperature: 35 DEG C
Experimental result:
See Fig. 7, compared to comparative example formula, formula of the present invention promptly can promote the concentration of Co-Q10 in rat plasma.Table 2 shows the plasma pharmacokinetics coefficient of rat after feeding 60mg/kg Co-Q10 (plasma pharmacokinetic coefficients), Cmax (the peak plasma concentration, C in the blood plasma of the Co-Q10 measured by the rat of wherein being filled a prescription with the present invention by feeding
max), TG-AUC (area undercurve, AUC) all apparently higher than by feeding with the rat of comparative example have.These experiment fruits demonstrate the oral absorption rate that the Co-Q10 formula utilizing microemulsion of the present invention to prepare can promote Co-Q10 really.Can knowing by inference thus, can be used to the oil-in-water microemulsion of preparation containing oil-soluble bioactivator for being applied in health beverages according to microemulsion of the present invention.
The plasma pharmacokinetics coefficient of table 2. rat after feeding 60mg/kg Co-Q10
Embodiment 5. prepares the oil-in-water microemulsion (Preparation ofcurcumin-containing o/w microemulsion) containing curcumin
The present embodiment prepares with reference to embodiment 2 oil-in-water microemulsion that contains curcumin.Experimental technique:
Use a ultrasonic vibrating cleaning machine (Delta Ultrasonic Cleaner DC400), with ultrasonic mode of oscillation, the curcumin of 500mg is dissolved in the coconut oil of 6g equably.Then utilize magnetite agitating mode to be added in curcumin/coconut oil blend by the Tween80 of 24g, after complete Homogeneous phase mixing, add deionized water to summation be again 100g and stir until exist without colloid substance in mixture with magnetite constantly.Obtained product is analyzed with particle size analyzer.
Experimental result:
See Fig. 8, the product that the present embodiment obtains is an orange oil-in-water microemulsion containing 5mg/g curcumin, and it is measured to has the average grain diameter that is about 45.7nm.The microemulsion containing curcumin that the present embodiment obtains also can be diluted to any concentration with an aqueous matrix.
Embodiment 6. prepares the oil-in-water microemulsion (Preparation of β-carotene-containing o/w microemulsion) containing beta carotene
The present embodiment prepares with reference to embodiment 2 oil-in-water microemulsion that contains beta carotene.
Experimental technique:
Use a ultrasonic vibrating cleaning machine (Delta Ultrasonic Cleaner DC400), with ultrasonic mode of oscillation, the beta carotene of 120mg is dissolved in the coconut oil of 6g equably.Then utilize magnetite agitating mode to be added in beta carotene/coconut oil blend by the Tween80 of 24g, after complete Homogeneous phase mixing, add deionized water to total amount be again 100g and stir until exist without colloid substance in mixture with magnetite constantly.Obtained product is analyzed with particle size analyzer.
Experimental result:
See Fig. 9, the product that the present embodiment obtains is an orange oil-in-water microemulsion containing 1.2mg/g beta carotene, and it is measured to has the average grain diameter that is about 47.1nm.The present embodiment the microemulsion obtained containing beta carotene also can be diluted to any concentration with an aqueous matrix.
The all patents quoted in this case description and data in literature and when described in reference data be merged in this case using as with reference to data with their entirety.If conflict to some extent, be as the criterion with the detailed description of this case (comprising in being defined in).
Although the present invention explains with regard to its specific embodiments, what can recognize is, it can do further modification, and the request of this part application case can contain any change of the present invention, purposes or modification, they are normally followed principle of the present invention and stem from the usual embodiment in field belonging to the present invention described in comprising and can be applied the change of essential feature described above, and drop in the category of claim of the application.
Claims (19)
1. the microemulsion preconcentrates containing a lipophilicity functional composition, is characterized in that: this microemulsion preconcentrates forms an oil-in-water microemulsion when diluting with an aqueous medium, and this microemulsion preconcentrates is be made up of following substantially:
One microemulsion be made up of coconut oil and polysorbate80, wherein coconut oil is 1:4 to 1:9 relative to the weight ratio of polysorbate80, and
One is dissolved in the lipophilicity functional composition in this microemulsion, and wherein this lipophilicity functional composition is be not more than 10 relative to the weight ratio of this microemulsion.
2. microemulsion preconcentrates according to claim 1, is characterized in that: this lipophilicity functional composition is drop in the scope of 1:10000 to 1:10 relative to the weight ratio of this microemulsion.
3. microemulsion preconcentrates according to claim 1, is characterized in that: this lipophilicity functional composition is Co-Q10, curcumin, carotenoid, astacin, Lycopene, resveratrol, DHA, or their combination.
4. microemulsion preconcentrates according to claim 1, is characterized in that: coconut oil is 1:4 relative to the weight ratio of polysorbate80.
5. microemulsion preconcentrates according to claim 1, is characterized in that: coconut oil is 1:9 relative to the weight ratio of polysorbate80.
6. the oil-in-water microemulsion that confession is oral, is characterized in that: this oil-in-water microemulsion is by diluting a microemulsion preconcentrates as claimed in claim 1 with an aqueous medium and obtained.
7. oil-in-water microemulsion according to claim 6, is characterized in that: this aqueous medium is pure water, deionized water, mineral water, fruit juice, vegetable juice, carbonated drink, cola, sarsaparilla, cow's milk, Yoghourt, soya-bean milk, beverage tea or sports drink.
8. a health beverages, is characterized in that: this health beverages contains an oil-in-water microemulsion as claimed in claim 6.
9. contain a method for the microemulsion preconcentrates of a lipophilicity functional composition for the preparation of one, it is characterized in that: the method comprises:
Carry out Homogeneous phase mixing one lipophilicity functional composition with coconut oil, Homogeneous phase mixing is with polysorbate80 then,
Wherein coconut oil is 1:4 to 1:9 relative to the weight ratio of polysorbate80, and this lipophilicity functional composition is be not more than 10 relative to the weight ratio of the summation of coconut oil and polysorbate80.
10. method according to claim 9, is characterized in that: this lipophilicity functional composition is drop in the scope of 1:10000 to 1:10 relative to the weight ratio of the summation of coconut oil and polysorbate80.
11. methods according to claim 9, is characterized in that: this lipophilicity functional composition is Co-Q10, curcumin, carotenoid, astacin, Lycopene, resveratrol, DHA or their combination.
12. methods according to claim 9, is characterized in that: coconut oil is 1:4 relative to the weight ratio of polysorbate80.
13. methods according to claim 9, is characterized in that: coconut oil is 1:9 relative to the weight ratio of polysorbate80.
14. 1 kinds supply the method for oral oil-in-water microemulsion for the preparation of one, it is characterized in that: the method comprises:
Carry out Homogeneous phase mixing one lipophilicity functional composition with coconut oil, Homogeneous phase mixing is with polysorbate80 then, and a microemulsion preconcentrates is formed, and
This microemulsion preconcentrates is diluted with an aqueous medium,
Wherein coconut oil is 1:4 to 1:9 relative to the weight ratio of polysorbate80, and this lipophilicity functional composition is be not more than 10 relative to the weight ratio of the summation of coconut oil and polysorbate80.
15. methods according to claim 14, is characterized in that: this lipophilicity functional composition is drop in the scope of 1:10000 to 1:10 relative to the weight ratio of the summation of coconut oil and polysorbate80.
16. methods according to claim 14, is characterized in that: this lipophilicity functional composition is Co-Q10, curcumin, carotenoid, astacin, Lycopene, resveratrol, DHA or their combination.
17. methods according to claim 14, is characterized in that: this aqueous medium is pure water, deionized water, mineral water, fruit juice, vegetable juice, carbonated drink, cola, sarsaparilla, cow's milk, Yoghourt, soya-bean milk, beverage tea or sports drink.
18. methods according to claim 14, is characterized in that: coconut oil is 1:4 relative to the weight ratio of polysorbate80.
19. methods according to claim 14, is characterized in that: coconut oil is 1:9 relative to the weight ratio of polysorbate80.
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| CN107568731A (en) * | 2017-10-19 | 2018-01-12 | 李宏 | A kind of Co-Q10 fish oil nanoemulsions and preparation method and application |
| CN107568731B (en) * | 2017-10-19 | 2020-09-29 | 李宏 | Coenzyme Q10 fish oil nano emulsion and preparation method and application thereof |
| US11786484B2 (en) | 2018-07-11 | 2023-10-17 | Aquanova Ag | Xanthohumol solubilizate |
| TWI701046B (en) * | 2019-05-14 | 2020-08-11 | 國泰醫療財團法人國泰綜合醫院 | Drug delivery system |
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