CN104719269B - It is a kind of to efficiently separate the method for collecting flour mite - Google Patents
It is a kind of to efficiently separate the method for collecting flour mite Download PDFInfo
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- CN104719269B CN104719269B CN201510169176.4A CN201510169176A CN104719269B CN 104719269 B CN104719269 B CN 104719269B CN 201510169176 A CN201510169176 A CN 201510169176A CN 104719269 B CN104719269 B CN 104719269B
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Abstract
The present invention provides a kind of method for efficiently separating and collecting flour mite, feed a part is screened out by sub-sieve slightly to be carried, the flour mite density of unit area is improved, mite body, the method that flour mite is collected in last centrifugation is trapped by being soaked with the fusiformis paper products or cloth products of a small amount of aqueous feed solution afterwards.The method that the present invention is provided is simple, time saving, efficient, and to mite body fanout free region, pollution-free, can be clean flour mite and other mite class, small insects etc. are separated and collected.
Description
Technical field
The present invention relates to agricultural and medical domain, more particularly, to a kind of method for efficiently separating and collecting flour mite.
Background technology
It is Acari, itch mite mesh, Acaroidea that flour mite is under the jurisdiction of, and species is various, widely distributed.They perch multiply in
In human habitat, Partial Species are close with human health and medical relationships.They endanger reserve, make its quality decline,
Use value is even lost completely, and very big economic loss is caused to the mankind；Some species can also parasitize the body surface and body of people
It is interior, cause the acariasis of respective organization and organ；The excreta of some species, secretion are extremely strong allergens, such as dust mite and
Dermatophagoides pteronyssinus, can cause the anaphylactia of people.Therefore, the understanding and research work for flour mite are most important.
The acquirement of research material is the basis of research work.Usually needed flour mite from feed or storage in real work
Separated in thing.Existing separation method played an important role in real work, but there is also many disadvantages.Such as
Direct Microscopical Method For Detection, time-consuming, and efficiency is low to be operated, it is necessary to cultivate skilled worker.Influence experiment progress.Additionally, there may be and artificially choose
Larger individuality is taken, is prone to ignore the factor of medium and small individuality, influence the accuracy of experimental result.Shake sieve separation method, efficiency high
In artificial picking, but when there is mite body and close feed granules size, feed granules are mixed with the selection result, it is impossible to clean
Mite is separated, and needs a large amount of clean mite bodies in actual research work sometimes.Electric heating collection mite method is time-consuming more long, collects mite body
Liquid trap is needed, makes mite body be subject to different degrees of damage and liquid to pollute.Existing all multi-methods when in use more to be present
Time-consuming, laborious, the low problem of efficiency.
The content of the invention
For above-mentioned the deficiencies in the prior art, the present invention provides a kind of method for efficiently separating and collecting flour mite, overcomes existing
Time-consuming when being used with the presence of the method, problem that laborious, efficiency is low, while clean can separate mite.
To achieve these goals, the present invention provides a kind of method for efficiently separating and collecting flour mite, comprises the following steps：
Step one：Slightly carry：Feed is screened by the sub-sieve of 60-120 mesh, the acarid for improving unit area is close
Degree；
Step 2：Trap：The feed containing flour mite is placed in an empty container for salable, wealthy mouth near edge after slightly carrying
One end, lure mite thing luring mite thing carrier one end to infiltrate, and mite thing carrier will be lured to be placed in empty container with respect to the feed containing flour mite
The other end of placement location, places under the conditions of empty container then is placed in into humidity 70-90%, 15-30 DEG C of temperature；
Step 3：Taken out after 1-3h and lure mite thing carrier, by containing luring one end of mite thing to be placed in centrifuge tube, the other end with from
Heart lid is fixedly connected, then centrifuge tube is put into centrifuge, is worked 5-10 minutes in 2500-3500rpm, you can by clean mite body
Spinning in pipe bottom.
Preferably, the described mite thing that lures is flour mite aggregation pheromone or aqueous feed solution.Mite is set to assemble according to predeterminated target,
And injury-free and pollution.
Preferably, the described mite thing carrier that lures is paper products or cloth products.There are certain toughness, intensity as carrier using them
And adsorptivity, and meet a small amount of water and will not disperse.Preferably, described paper products or cloth products are spindle shape.After being conducive to trapping
Centrifugation tube hub is fixed on, mite body is efficiently separated.Preferably, it is described to lure mite thing carrier for cloth products, in the step 3
3000rpm works 10 minutes.Overlong time, easily causes to damage to mite body, and too short separation is not thorough enough.Preferably, it is described to lure
Mite thing carrier is paper products, is worked 5 minutes in 2500rpm in the step 3.What mite body was separate under the conditions of this is relatively totally more thorough
Bottom, while avoiding damage to mite body.
Preferably, the floor space of the step 2 hollow container is 100-1000cm2, apart from being closely unfavorable for very much separating, too
Remote mite body is to the reduction of decoy sensitiveness.
Preferably, empty container is placed in humidity 75-80%, 25-30 DEG C of placement of temperature in the step 2.This humiture bar
Mite body is more active under part, traps separating effect preferable.
Preferably, it is that other end insertion centrifugation lid is prior that the other end is fixedly connected with centrifugation lid in the step 3
It is fixed in the aperture being drilled.
Described method can be applied to that spider mite kind and small insects are collected by centrifugation.To directly have mite class or insect blade or
Plantlet is fixed on upper end in centrifuge tube and is collected by centrifugation.
Beneficial effect：The method that the present invention is provided is simple, time saving, efficient, and to mite body fanout free region, pollution-free, can
Clean is separated and collected flour mite and other mite class, small insects etc..
Specific embodiment
With reference to specific embodiment, the present invention is expanded on further.Specific embodiment of the invention is each as involved by
The shape of component, the mutual alignment between construction, each several part and annexation, the effect of each several part and operation principle, manufacture work
Skill and operate with method etc., are described in further detail, with help those skilled in the art to inventive concept of the invention,
Technical scheme has more complete, accurate and deep understanding.
It is a kind of to efficiently separate the method for collecting flour mite, comprise the following steps：
Step one：Slightly carry：Feed is screened by the sub-sieve of 60-120 mesh, the acarid for improving unit area is close
Degree；
Step 2：Trap：The feed containing flour mite is placed in an empty container for salable, wealthy mouth near edge after slightly carrying
One end, lure mite thing luring mite thing carrier one end to infiltrate, and mite thing carrier will be lured to be placed in empty container with respect to the feed containing flour mite
The other end of placement location, places under the conditions of empty container then is placed in into humidity 70-90%, 15-30 DEG C of temperature；
Step 3：Taken out after 1-3h and lure mite thing carrier, by containing luring one end of mite thing to be placed in centrifuge tube, the other end with from
Heart lid is fixedly connected, it is preferred that being will be fixed in the other end insertion prior aperture being drilled of centrifugation lid, then centrifuge tube is put
Enter centrifuge, worked 5-10 minutes in 2500-3500rpm, you can by clean mite body spinning in pipe bottom.
It is flour mite aggregation pheromone or aqueous feed solution wherein to lure mite thing.Mite is assembled according to predeterminated target, and be not damaged
Wound and pollution.Preferably, it is paper products or cloth products to lure mite thing carrier.There are certain toughness, intensity and suction as carrier using them
Attached property, and meet a small amount of water and will not disperse.Preferably, paper products or cloth products are spindle shape.Centrifugation is fixed on after being conducive to trapping
Tube hub, efficiently separates mite body.Preferably, when luring mite thing carrier for cloth products, worked 10 minutes in 3000rpm in step 3.
Overlong time, easily causes to damage to mite body, and too short separation is not thorough enough.Preferably, it is paper products step 3 to lure mite thing carrier
In 2500rpm work 5 minutes.What mite body was separate under the conditions of this is relatively totally more thorough, while avoiding damage to mite body.In step 2
The floor space of empty container is 100-1000cm2, apart from being closely unfavorable for very much separating, too remote mite body is to the reduction of decoy sensitiveness.Step
Empty container is placed in humidity 75-80%, 25-30 DEG C of placement of temperature in rapid two.Mite body is more active under this temperature and humidity conditions, traps
Separating effect is preferable.
The method for efficiently separating collection flour mite can be applied to that spider mite kind and small insects are collected by centrifugation.To directly have mite class or
The blade or plantlet of insect are fixed on upper end in centrifuge tube and are collected by centrifugation.
Below by specific preferred embodiment, the present invention will be further described.
Embodiment one：Separate the tyrophagus putrescentiae raised in dusty yeast
Step one：Slightly carry：Feed is screened by the sub-sieve of 100 mesh, improves the acarid density of unit area；
Step 2：Trap：The feed containing tyrophagus putrescentiae is placed in salable, wealthy mouth, a floor space and is after slightly carrying
174cm2The empty container of (culture dish of diameter 15cm) (is cut into the filter paper of spindle shape near the one end at edge mite thing carrier is lured
Piece) one end infiltration lures mite thing (aqueous feed solution supernatant), and mite thing carrier will be lured to be placed in empty container with respect to the feed containing flour mite
The other end of placement location, places under the conditions of empty container then is placed in into humidity 75%, 25 DEG C of temperature；
Step 3：Taken out after 2h and lure mite thing carrier, by containing luring one end of mite thing to be placed in 2ml centrifuge tubes, the other end is inserted
Enter to be centrifuged fixation in the aperture that lid is drilled in advance.Centrifuge tube is put into centrifuge again, is worked 5 minutes in 2500rpm, you can will
Clean mite body spinning in pipe bottom, and the injury minimum suffered by mite body.The mite body quantity that the method is collected into is through 3 repetitions, system
It is calculated as 274 ± 36.
Embodiment two：Separate the tyrophagus putrescentiae raised in wheat flour
Step one：Slightly carry：Feed is screened by the sub-sieve of 100 mesh, improves the acarid density of unit area；
Step 2：Trap：The feed containing tyrophagus putrescentiae is placed in salable, wealthy mouth, a floor space and is after slightly carrying
452cm2The empty container of (diameter 24cm's dries vessel) (is cut into the nothing of spindle shape near the one end at edge mite thing carrier is lured
Spin cloth) one end infiltration lures mite thing (aqueous feed solution supernatant), and mite thing carrier will be lured to be placed in empty container with respect to the feeding containing flour mite
Expect the other end of placement location, placed under the conditions of empty container then is placed in into humidity 75%, 25 DEG C of temperature；
Step 3：Taken out after 3h and lure mite thing carrier, by containing luring one end of mite thing to be placed in 2ml centrifuge tubes, the other end is inserted
Enter to be centrifuged fixation in the aperture that lid is drilled in advance.Centrifuge tube is put into centrifuge again, is worked 10 minutes in 3000rpm, you can
By clean mite body spinning in pipe bottom, and the injury minimum suffered by mite body.The mite body quantity that the method is collected into through 3 repetitions,
Count is 220 ± 53.
Embodiment three：Separate the rotten Bai Shi life card mites raised in dusty yeast
Step one：Slightly carry：Feed is screened by the sub-sieve of 80 mesh, improves the acarid density of unit area；
Step 2：Trap：Feed containing corruption Bai Shi life card mites after slightly carrying is placed in salable, wealthy mouth, a floor space
It is 174cm2The empty container of (culture dish of diameter 15cm) (is cut into the filter of spindle shape near the one end at edge mite thing carrier is lured
The scraps of paper) one end infiltration lures mite thing (aqueous feed solution supernatant), and mite thing carrier will be lured to be placed in empty container with respect to the feeding containing flour mite
Expect the other end of placement location, placed under the conditions of empty container then is placed in into humidity 80%, 30 DEG C of temperature；
Step 3：Taken out after 3h and lure mite thing carrier, by containing luring one end of mite thing to be placed in 2ml centrifuge tubes, the other end is inserted
Enter to be centrifuged fixation in the aperture that lid is drilled in advance.Centrifuge tube is put into centrifuge again, is worked 8 minutes in 3500rpm, you can will
Clean mite body spinning in pipe bottom, and the injury minimum suffered by mite body.The mite body quantity that the method is collected into is through 3 repetitions, system
It is calculated as 198 ± 29.
The present invention is exemplarily described above, it is clear that the present invention is implemented and is not subject to the restrictions described above,
As long as employing the improvement of various unsubstantialities that method of the present invention design and technical scheme are carried out, or not improved this is sent out
Bright design and technical scheme directly applies to other occasions, within protection scope of the present invention.
Claims (7)
1. it is a kind of to efficiently separate the method for collecting flour mite, comprise the following steps：
Step one：Slightly carry：Feed is screened by the sub-sieve of 60-120 mesh；
Step 2：Trap：The feed containing flour mite is placed in an empty container for salable, wealthy mouth near the one of edge after slightly carrying
End, mite thing is lured luring mite thing carrier one end to infiltrate, and mite thing carrier will be lured to be placed in the relative feed placement containing flour mite of empty container
The other end of position, places under the conditions of empty container then is placed in into humidity 70-90%, 15-30 DEG C of temperature；The bottom of the empty container
Area is 100-1000cm2；
Step 3：Taken out after 1-3h and lure mite thing carrier, by containing luring one end of mite thing to be placed in centrifuge tube, the other end and centrifuge tube
Lid is fixedly connected, then centrifuge tube is put into centrifuge, is worked 5-10 minutes in 2500-3500rpm, you can screw in clean mite body
Ttom of pipe；It is that will be fixed in the other end insertion prior aperture being drilled of centrifugation lid that the other end is fixedly connected with centrifugation lid.
It is 2. according to claim 1 to efficiently separate the method for collecting flour mite, it is characterised in that：The described mite thing that lures is flour mite
Aggregation pheromone or aqueous feed solution.
It is 3. according to claim 1 to efficiently separate the method for collecting flour mite, it is characterised in that：The described mite thing carrier that lures is
Paper products or cloth products.
It is 4. according to claim 3 to efficiently separate the method for collecting flour mite, it is characterised in that：Described paper products or fabric
Product are spindle shape.
It is 5. according to claim 3 to efficiently separate the method for collecting flour mite, it is characterised in that：It is described to lure mite thing carrier for cloth
Product, works 10 minutes in the step 3 in 3000rpm.
It is 6. according to claim 3 to efficiently separate the method for collecting flour mite, it is characterised in that：It is described to lure mite thing carrier for paper
Product, works 5 minutes in the step 3 in 2500rpm.
It is 7. according to claim 1 to efficiently separate the method for collecting flour mite, it is characterised in that：Sky is held in the step 2
Device is placed in humidity 75-80%, 25-30 DEG C of placement of temperature.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510169176.4A CN104719269B (en) | 2015-04-10 | 2015-04-10 | It is a kind of to efficiently separate the method for collecting flour mite |
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| CN201510169176.4A CN104719269B (en) | 2015-04-10 | 2015-04-10 | It is a kind of to efficiently separate the method for collecting flour mite |
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| CN104719269A CN104719269A (en) | 2015-06-24 |
| CN104719269B true CN104719269B (en) | 2017-06-09 |
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Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105124229A (en) * | 2015-08-24 | 2015-12-09 | 南昌大学 | Artificial feed for flour mites |
Citations (4)
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| CN101337077A (en) * | 2008-08-20 | 2009-01-07 | 山东省寄生虫病防治研究所 | Preparation method of skin spot pricking method diagnosis lung acariasis antigen liquid |
| CN202618079U (en) * | 2012-05-10 | 2012-12-26 | 有限公司日革研究所 | Mite catching device |
| CN202857586U (en) * | 2012-10-29 | 2013-04-10 | 贵州大学 | Simple and efficient flour mite purification breeding device |
| CN104472455A (en) * | 2014-12-02 | 2015-04-01 | 贵州大学 | Large-size predacious mite collection method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7051672B2 (en) * | 2004-02-17 | 2006-05-30 | Syngenta Participations Ag | System for providing beneficial insects or mites |
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2015
- 2015-04-10 CN CN201510169176.4A patent/CN104719269B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101337077A (en) * | 2008-08-20 | 2009-01-07 | 山东省寄生虫病防治研究所 | Preparation method of skin spot pricking method diagnosis lung acariasis antigen liquid |
| CN202618079U (en) * | 2012-05-10 | 2012-12-26 | 有限公司日革研究所 | Mite catching device |
| CN202857586U (en) * | 2012-10-29 | 2013-04-10 | 贵州大学 | Simple and efficient flour mite purification breeding device |
| CN104472455A (en) * | 2014-12-02 | 2015-04-01 | 贵州大学 | Large-size predacious mite collection method |
Non-Patent Citations (2)
| Title |
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| "粉尘螨和屋尘螨饲养及分离技术研究进展";裴伟等;《中国病原生物学杂志》;20090831;第4卷(第8期);第633-634页 * |
| "粉螨类分离纯化的简便法";秦剑等;《济宁医学院学报》;19931031;第17页 * |
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