CN104711193A - Method for destroying cell structure of aquatic product - Google Patents
Method for destroying cell structure of aquatic product Download PDFInfo
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- CN104711193A CN104711193A CN201410366022.XA CN201410366022A CN104711193A CN 104711193 A CN104711193 A CN 104711193A CN 201410366022 A CN201410366022 A CN 201410366022A CN 104711193 A CN104711193 A CN 104711193A
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- 238000000034 method Methods 0.000 title claims abstract description 63
- 239000000463 material Substances 0.000 claims abstract description 65
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 30
- 239000002002 slurry Substances 0.000 claims abstract description 24
- 239000002253 acid Substances 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims description 29
- 239000000126 substance Substances 0.000 claims description 27
- 239000002994 raw material Substances 0.000 claims description 23
- 230000006378 damage Effects 0.000 claims description 10
- 230000008676 import Effects 0.000 claims description 5
- 210000004027 cell Anatomy 0.000 description 48
- 239000007789 gas Substances 0.000 description 25
- 241000195493 Cryptophyta Species 0.000 description 21
- 230000000052 comparative effect Effects 0.000 description 19
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 210000003850 cellular structure Anatomy 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 235000011089 carbon dioxide Nutrition 0.000 description 3
- 238000005265 energy consumption Methods 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 238000012423 maintenance Methods 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 239000000470 constituent Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000002309 gasification Methods 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 235000016425 Arthrospira platensis Nutrition 0.000 description 1
- 240000002900 Arthrospira platensis Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 1
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 1
- 235000013734 beta-carotene Nutrition 0.000 description 1
- 239000011648 beta-carotene Substances 0.000 description 1
- 229960002747 betacarotene Drugs 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229930002875 chlorophyll Natural products 0.000 description 1
- 235000019804 chlorophyll Nutrition 0.000 description 1
- 229940106705 chlorophyll Drugs 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 229940082787 spirulina Drugs 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 230000008016 vaporization Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a method for destroying the cell structure of aquatic products. The method comprises the following steps: a providing an aquatic material feed stock, the aquatic material feed stock comprising an aquatic material; b adjusting the water content of the aquatic product material to form an aquatic product slurry to be treated; c placing the aquatic product slurry to be treated in a pressure container; d, introducing compressed gas into the pressure container to enable the compressed gas and water in the aquatic product slurry to be treated to form acid liquor, and enabling the cell structure of the aquatic product to be hydrolyzed and damaged through the acid liquor; e a depressurization step to separate the compressed gas.
Description
Technical field
The present invention about a kind of method making cellularstructure destroy, especially in regard to a kind of method making the structure deteriorate of aquatic products Materials Cell.
Background technology
Aquatic products material, as algae etc., because growing fast and absorbing carbon dioxide, has the effect of solid carbon, therefore has become popular raw mass-energy, food, feed, medicine source now.Energy, after carrying out photosynthesis, can store in the mode of protein, carbohydrate or grease by algae.Algae, mushroom have abundant health-care components, as β-carotene, chlorophyll, linolenic acid, Phycocyanins, C-, VITAMIN, carbohydrate etc., have good medical care effect.But, before edible, extraction, hydrolysis algae and mushroom composition, usually must destroy its cellularstructure, specific absorption, percentage extraction or percent hydrolysis could be improved.
Existing cellularstructure destruction methods is as following technical patent document analysis.
1、TW542699
Way: utilize pressure 2-10kg/cm
2, temperature 100-150 DEG C gas, make ICW reach gasification stagnation point, and moment step-down makes the gasification of ICW break through cell walls.
Shortcoming: use high-temperature technology energy consumption high; High temperature easily makes the contained effectively component damage of cell decompose; And blast noise can be produced in technique.
2、US7763724
Way: under 500MPa-1000MPa hydraulic pressure and temperature are the condition of 60-80 DEG C, makes the cellularstructure of marine alga liquefy and produces glucose.
Shortcoming: operational condition is more than 500MPa ultra-high voltage, need to process in thick and heavy pressure-bearing structure equipment, its equipment builds with maintenance cost high, and the technique of unfavorable business running amplifies use.
Based on above-mentioned analysis, be necessary to provide an innovation and the method making the structure deteriorate of aquatic products Materials Cell of tool progressive, to solve above-mentioned disappearance of the prior art.
Summary of the invention
The invention provides a kind of method making the structure deteriorate of aquatic products Materials Cell, comprise the following steps:
A provides aquatic products raw material of substance, and this aquatic products raw material of substance comprises aquatic products material;
B adjusts the water content of this aquatic products raw material of substance, to form pending aquatic products material slurries;
These pending aquatic products material slurries are placed in pressurized vessel by c;
Pressurized gas imports in this pressurized vessel by d, makes the moisture in this pressurized gas and this pending aquatic products material slurries form acid solution, and makes aquatic products Materials Cell structure be hydrolyzed destruction by this acid solution;
E carries out depressurization step, to be separated this pressurized gas.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the weight percent concentration of the pending aquatic products material slurries described in step b is 0.4 to 25wt%.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the water content of step b Bao Kuo Minus this aquatic products raw material of substance few.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, step b comprises the water content increasing this aquatic products raw material of substance.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, step c comprises makes described pending aquatic products material slurries in this pressurized vessel, form the step of aquatic products substance film.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the thickness of described aquatic products substance film is not more than 2 centimetres.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the gauge pressure of the pressurized gas described in steps d is not less than 10bar.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the gauge pressure of this pressurized gas is 30 to 200bar.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the holding time of pressure of the pressurized gas described in steps d is not less than 1 minute.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the holding time of pressure of described pressurized gas is 15 minutes to 48 hours.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the service temperature of the pressurized vessel described in steps d is not more than 55 DEG C.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the pH value of described acid solution is 2 to 6.5.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, steps d also comprises step pressurized liquid being imported this pressurized vessel.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, steps d also comprises step pressurized liquid being imported this pressurized vessel, to be stressed destruction to make aquatic products Materials Cell structure.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the pressure of described pressurized liquid is not less than the pressure of described pressurized gas.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the gauge pressure of described pressurized liquid is 100 to 4000bar.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the holding time of pressure of described pressurized liquid is not less than 1 minute.
Make in the method for aquatic products Materials Cell structure deteriorate provided by the present invention, preferably, the holding time of pressure of described pressurized liquid is 15 minutes to 48 hours.
Method of the present invention can operate at low ambient temperatures, therefore can reduce energy consumption and prevent effective constituent contained by cell from decomposed by high temperature.In addition, the present invention can implement under lower pressure condition, therefore, need not use thick and heavy high-tension apparatus, and its equipment builds and maintenance cost can significantly reduce, and the technique being applicable to business running amplifies use.
Accompanying drawing explanation
Fig. 1 is the method flow diagram making the structure deteriorate of aquatic products Materials Cell provided by the invention;
Fig. 2 is the experiment flow figure of comparative example 1;
Fig. 3 is the experiment flow figure of embodiment 1;
Fig. 4 is the electron microscope picture after the alga cells broken wall of comparative example 1;
Fig. 5 is the electron microscope picture after the alga cells broken wall of embodiment 1.
Embodiment
In order to technique means of the present invention can be better understood, and can be implemented according to the content of specification sheets, and in order to object of the present invention, feature and advantage can be become apparent, below especially exemplified by preferred embodiment, and coordinated accompanying drawing, be described in detail as follows.
Fig. 1 is the method flow diagram that the present invention makes the structure deteriorate of aquatic products Materials Cell.Consult the step S11 of Fig. 1, provide aquatic products raw material of substance, this aquatic products raw material of substance comprises aquatic products material.This aquatic products material is optional from following wherein one: algae and mushroom.
In this step, algae is optional from following wherein one: micro-algae and marine alga.Preferably, the size range of micro-algae is 3 to 30 microns, and micro-algae is optional from following wherein a kind of: green alga, diatom, spirulina, ball algae and draw algae.The cultivation concentration of general micro-algae is less than 0.3wt%, and after the moisture such as high centrifuge dehydration, drying remove means, concentration can more than 25wt%, and concentration is too low or too highly unfavorablely carry out cellularstructure with pressurized gas and destroy process.
Cf. steps S12, adjusts the water content of this aquatic products raw material of substance, to form pending aquatic products material slurries.When initial condition product raw material is containing large quantity of moisture, the mode of adjustment water content is reduce the water content of this aquatic products raw material of substance, to adjust the weight percent concentration of aquatic products material slurries for 0.4 to 25wt%, easily spreads to make gas.And the method reducing water content is optional from following wherein one: sedimentation, centrifugal and filtration.But when initial condition product raw material moisture content is too low, the mode of adjustment water content is then for increasing the water content of this aquatic products raw material of substance.
These pending aquatic products material slurries are placed in pressurized vessel by cf. steps S13.In this step, for improving the processing area of these pending aquatic products material slurries in this pressurized vessel, these pending aquatic products material slurries can be made in this pressurized vessel to form aquatic products substance film, and the method forming this aquatic products substance film is optional from following wherein a kind of: scraper membrane formation process and be sprayed into embrane method.And preferably, the thickness of this aquatic products substance film is not more than 2 centimetres.
Cf. steps S14, imports pressurized gas in this pressurized vessel, makes the moisture in this pressurized gas and this pending aquatic products material slurries form acid solution, and makes aquatic products Materials Cell structure be hydrolyzed destruction by this acid solution.In this step, this compressed gas know from experience form acid with the moisture of these pending aquatic products material slurries and with the cells contacting of aquatic products material, promote that cellularstructure degraded destroys.In addition, the concentration of this pressurized gas in water can improve gradually along with the growth of time and fall low-moisture pH value.Preferably, the pH value of this acid solution is 2 to 6.5, and the service temperature of this pressurized vessel is not more than 55 DEG C.
In this step, this pressurized gas comprises one or both the combination in carbonic acid gas, methane and nitrogen.In addition, the gauge pressure of this pressurized gas is not less than 10bar, and to make the pH value of this acid solution control 2 to 6.5, and holding time of the pressure of pressurized gas is not less than 1 minute.Preferably, the gauge pressure of this pressurized gas is 30 to 200bar, and holding time of the pressure of this pressurized gas is 15 minutes to 48 hours.
In addition, the cellularstructure hydrolysis of this aquatic products material can be accelerated by improving acid solution amount and improve sporoderm-broken rate, therefore, in this step, pressurized liquid can be imported in this pressurized vessel, to improve the internal pressure of this pressurized vessel, and aquatic products Materials Cell structure is stressed destruction.In addition, by changing the pressure of this pressurized liquid, the effect of stress application can be produced repeatedly the cellularstructure of this aquatic products material, cyto-architectural destruction can be accelerated equally.
In this step, this pressurized liquid comprises the combination of one or more in water, methyl alcohol, ethanol and vegetables oil.In addition, the pressure of this pressurized liquid is not less than the pressure of this pressurized gas, and holding time of the pressure of pressurized liquid is not less than 1 minute.Preferably, the gauge pressure of this pressurized liquid is 100 to 4000bar, and holding time of the pressure of this pressurized liquid is 15 minutes to 48 hours.
Cf. steps S15, carries out depressurization step, to be separated this pressurized gas.The Pressure Drop of this pressurized vessel to gauge pressure is zero by this depressurization step, and vaporizing to make this pressurized gas is separated, and then recyclable recycling.
Method of the present invention can operate at low ambient temperatures, therefore can reduce energy consumption and prevent effective constituent contained by cell from decomposed by high temperature.In addition, the present invention can implement under lower pressure condition, therefore, need not use thick and heavy high-tension apparatus, and its equipment builds and maintenance cost can significantly reduce, and the technique being applicable to business running amplifies use.
Comparative example 1
Consult Fig. 2, it is the experiment flow figure of comparative example 1.The aquatic products raw material of substance of comparative example 1 is algae liquid (concentration is 0.05 to 0.1wt% about), and pressurized gas is carbonic acid gas (CO
2), pressurized liquid is water.
This comparative example provides a kind of method making the structure deteriorate of aquatic products Materials Cell, and concrete steps are as follows:
Step 1: the algae liquid getting 60 milliliters, is placed in wide-necked bottle;
Step 2: aforementioned algae liquid is placed in pressurized vessel;
Step 3: be the CO of 50bar by gauge pressure
2import in this pressurized vessel, and hold pressure 1 minute, experimental temperature is 30 DEG C;
Step 4: water gauge pressure being respectively 1200bar, 2000bar and 4000bar imports in this pressurized vessel, and hold pressure 30 minutes; And
Step 5: step-down, completes experiment.
Embodiment 1
Consult Fig. 3, it is the experiment flow figure of embodiment 1.The aquatic products raw material of substance of embodiment 1 is algae liquid (concentration is 0.05 to 0.1wt% about), and pressurized gas is carbonic acid gas (CO
2), pressurized liquid is water.
Present embodiments provide a kind of method making the structure deteriorate of aquatic products Materials Cell, concrete steps are as follows:
Step 1: the algae liquid getting 60 milliliters, is placed in wide-necked bottle;
Step 2: aforementioned algae liquid is left standstill 0.5 hour, makes algae fall to bottom wide-necked bottle, remove the water of 55 milliliters afterwards, forms algae slurry;
Step 3: aforementioned algae slurry is placed in pressurized vessel, and make this algae starch at this internal pressure vessel walls formation film;
Step 4: be the CO of 50bar by gauge pressure
2import in this pressurized vessel, and keep pressure 15 minutes, 30 minutes and 1 hour respectively, experimental temperature is 30 DEG C;
Step 5: be that the water of 1200bar imports in this pressurized vessel by gauge pressure, and keep pressure 15 minutes, 30 minutes, 1 hour, 2 hours, 7 hours and 16 hours respectively; And
Step 6: step-down, completes experiment.
Embodiment 2
Present embodiments provide a kind of method making the structure deteriorate of aquatic products Materials Cell, concrete steps are as follows:
The experiment condition of embodiment 2 is substantially identical with embodiment 1, and its difference place is only that embodiment 2 omits the step 5 described in embodiment 1, and the time of holding pressure of step 4 is set as 20 minutes, 1 hour and 2 hours.
Comparative example 2
This comparative example provides a kind of method making the structure deteriorate of aquatic products Materials Cell, and concrete steps are as follows:
The experiment condition of comparative example 2 is substantially identical with embodiment 1, and its difference place is only that comparative example 2 eliminates the step 4 described in embodiment 1, and the time of holding pressure of step 5 is set as 1 hour, 2 hours and 4 hours.
Due to alga cells destroyed after can flow out ionogen, therefore its cell-wall breaking ratio can present positive correlation with conductance.Therefore, the present embodiment is that the alga cells understanding comparative example 1, embodiment 1, embodiment 2 and comparative example 2 destroys situation, and carry out sporoderm-broken rate measurement with conductance method, its measurement is as shown in table 1.
The sporoderm-broken rate measurement of table 1 comparative example 1, embodiment 1, embodiment 2 and comparative example 2
The water (be about algae liquid long-pending 92%) of result display comparative example 1 because not removing 55 milliliters of table 1, therefore its alga cells sporoderm-broken rate is only 30%.The reason that comparative example 1 sporoderm-broken rate is not good should be that the pH value of algae liquid itself was about for 9 (being equivalent to alkali lye), when algae liquid contains a large amount of alkaline water, and CO
2not easily spread, and be difficult to form carbonic acid.Consult Fig. 4, it is the electron microscope picture after comparative example 1 alga cells broken wall.As shown in Figure 4, the oil droplet outflow phenomenon after comparative example 1 alga cells broken wall is also not obvious.
On the contrary, embodiment 1 after removing the water of 55 milliliters, CO
2easy diffusion, and easily form carbonic acid, under the condition of Bound moisture supercharging, its alga cells sporoderm-broken rate can reach 95%; Even if omit water pressure increase step, its alga cells sporoderm-broken rate also can reach 78% (embodiment 2).
Consult Fig. 5, it is the electron microscope picture after embodiment 1 alga cells broken wall.As shown in Figure 5, the oil droplet outflow phenomenon after embodiment 1 alga cells broken wall is quite obvious.
In addition, if CO is omitted in the result display of comparative example 2
2pressure increase step, carbonic acid cannot be formed, so that cannot be hydrolyzed destruction to alga cells, even if carry out water pressure increase step again, its alga cells sporoderm-broken rate also can only reach 16%.
The initial condition product raw material of above-described embodiment, all containing large quantity of moisture, so the mode reducing water content must be adopted to adjust the water content of this aquatic products raw material of substance, could form pending aquatic products material slurries.But, when initial condition product raw material moisture content is too low, the undue thickness of aquatic products material slurries, lack moisture, then the mode increasing water content must be adopted to adjust the water content of this aquatic products raw material of substance, in order to formation substance film and improve sporoderm-broken rate.
Above-described embodiment is only and principle of the present invention and effect thereof is described, and unrestricted the present invention, therefore those skilled in the art modifies to above-described embodiment and changes and still do not depart from spirit of the present invention.
Claims (17)
1. make a method for aquatic products Materials Cell structure deteriorate, comprise the following steps:
A provides aquatic products raw material of substance, and this aquatic products raw material of substance comprises aquatic products material;
B adjusts the water content of this aquatic products raw material of substance, to form pending aquatic products material slurries;
These pending aquatic products material slurries are placed in pressurized vessel by c;
Pressurized gas imports in this pressurized vessel by d, makes the moisture in this pressurized gas and this pending aquatic products material slurries form acid solution, and makes aquatic products Materials Cell structure be hydrolyzed destruction by this acid solution;
E carries out depressurization step, to be separated this pressurized gas.
2. method according to claim 1, wherein, the weight percent concentration of the pending aquatic products material slurries described in step b is 0.4wt% to 25wt%.
3. method according to claim 1, wherein, the water content of step b Bao Kuo Minus this aquatic products raw material of substance few.
4. method according to claim 1, wherein, step b comprises the water content increasing this aquatic products raw material of substance.
5. method according to claim 1, wherein, step c comprises the step making these pending aquatic products material slurries form aquatic products substance film in this pressurized vessel.
6. method according to claim 5, wherein, the thickness of described aquatic products substance film is not more than 2 centimetres.
7. method according to claim 1, wherein, the gauge pressure of the pressurized gas described in steps d is not less than 10bar.
8. method according to claim 7, wherein, the gauge pressure of this pressurized gas is 30bar to 200bar.
9. method according to claim 7, wherein, the holding time of pressure of the pressurized gas described in steps d is not less than 1 minute.
10. method according to claim 9, wherein, the holding time of pressure of described pressurized gas is 15 minutes to 48 hours.
11. methods according to claim 1, wherein, the service temperature of the pressurized vessel described in steps d is not more than 55 DEG C.
12. methods according to claim 1, wherein, the pH value of described acid solution is 2 to 6.5.
13. methods according to claim 1, wherein, steps d also comprises step pressurized liquid being imported this pressurized vessel, to be stressed destruction to make aquatic products Materials Cell structure.
14. methods according to claim 13, wherein, the pressure of described pressurized liquid is not less than the pressure of described pressurized gas.
15. methods according to claim 14, wherein, the gauge pressure of described pressurized liquid is 100 to 4000bar.
16. methods according to claim 15, wherein, the holding time of pressure of described pressurized liquid is not less than 1 minute.
17. methods according to claim 16, wherein, the holding time of pressure of described pressurized liquid is 15 minutes to 48 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14/555,137 US9416346B2 (en) | 2013-12-11 | 2014-11-26 | Method of damaging cell structure of aquatic substance |
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW102145712 | 2013-12-11 | ||
| TW102145712A TWI544074B (en) | 2013-12-11 | 2013-12-11 | So that the aquatic cell structure damage method |
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| Publication Number | Publication Date |
|---|---|
| CN104711193A true CN104711193A (en) | 2015-06-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410366022.XA Pending CN104711193A (en) | 2013-12-11 | 2014-07-29 | Method for destroying cell structure of aquatic product |
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| CN (1) | CN104711193A (en) |
| TW (1) | TWI544074B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110183403A1 (en) * | 2008-08-07 | 2011-07-28 | Uhde High Pressure Technologies Gmbh | Cell disruption of plant and animal raw materials by a combination of automization process with decompression processes for selective extraction and separation of interacellular valuable substances |
-
2013
- 2013-12-11 TW TW102145712A patent/TWI544074B/en active
-
2014
- 2014-07-29 CN CN201410366022.XA patent/CN104711193A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110183403A1 (en) * | 2008-08-07 | 2011-07-28 | Uhde High Pressure Technologies Gmbh | Cell disruption of plant and animal raw materials by a combination of automization process with decompression processes for selective extraction and separation of interacellular valuable substances |
Non-Patent Citations (2)
| Title |
|---|
| ATSUSHI ENOMOTO ET AL: "Inactivation of food microorganisms by high-pressure carbon dioxide treatment with or without explosive decompression", 《BIOSCI.BIOTECH.BIOCHEM》 * |
| HO-MU LIN: "Disintegration of Yeast Cells by Pressurized Carbon Dioxide", 《BBTECHNOL. PROG》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| TWI544074B (en) | 2016-08-01 |
| TW201522619A (en) | 2015-06-16 |
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