CN104709988A - Novel flocculant and application thereof - Google Patents

Novel flocculant and application thereof Download PDF

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Publication number
CN104709988A
CN104709988A CN201510118484.4A CN201510118484A CN104709988A CN 104709988 A CN104709988 A CN 104709988A CN 201510118484 A CN201510118484 A CN 201510118484A CN 104709988 A CN104709988 A CN 104709988A
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novel flocculant
fermented liquid
flocculant
supernatant liquor
gram
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CN104709988B (en
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穆大帅
陈冠军
杜宗军
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Shandong University Weihai
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Shandong University Weihai
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

Abstract

The invention relates to the technical field of microorganism processing, in particular to a novel flocculant and application thereof. The novel flocculant is characterized in a water solution of nanometer aluminum oxide, and the water solution of the nanometer aluminum oxide is a 10% nanometer solution. The application comprises the following steps: innoculating a culture medium with a bacterial strain, and fermenting to obtain fermented bacteria; adding the novel flocculant into fermentation liquid, wherein the adding concentration is 0.1mmol/L to 20mmol/L; after homogenizing, standing for 30 minutes, and separating the fermentation bacteria and supernatant from the mixture. Compared with the prior art, the novel flocculant adopting nanometer inorganic metal oxide has the advantages of being nontoxic, high in flocculation efficiency, small in use quantity, convenient and rapid and has high popularization and application values.

Description

A kind of novel flocculant and application thereof
Technical field
The present invention relates to microbiological treatment technical field, specifically a kind of throwing out that can significantly improve thalline, thus conventional centrifugal method can be replaced, be specially adapted to the novel flocculant to the flocculation precipitation pretreatment of bacterium class fermented liquid and application thereof.
Background technology
As everyone knows, flocculation agent totally can be divided into inorganic flocculating agent and organic floculant two class according to its chemical composition.Wherein inorganic flocculating agent comprises again inorganic flocculant and inorganic polymer flocculant; Organic floculant comprises again Syn-Organic flocculants, natural organic high-molecular flocculant and microbial flocculant.
Inorganic flocculating agent comprises Tai-Ace S 150, aluminum chloride, ferric sulfate, iron(ic) chloride etc., and wherein Tai-Ace S 150 is developed by the U.S. the earliest, and a kind of important inorganic flocculating agent always used till today.Conventional aluminium salt has Tai-Ace S 150 Al 2(SO 4) 3.18H 2o and alum Al 2(SO 4) 3.K 2sO 4.24H 2o, another kind of is that molysite has Iron trichloride hexahydrate FeCl 3.6H 2o. ferrous sulfate hydrate FeSO 4.17H 2o and ferric sulfate.
Simple inorganic polymer flocculation agent, the polymkeric substance of this kind of inorganic polymer flocculation agent mainly aluminium salt and molysite.As polymerize aluminum chloride (PAC), polyaluminium sulfate (PAS), poly-ferric chloride (PFC) and bodied ferric sulfate (PFS) etc.Why effective than other inorganic flocculating agent inorganic polymer flocculation agent is, and its basic reason is that it can provide a large amount of complexing ions, and can strong adsorption colloidal particle, by absorption, crane span structure, crosslinked action, thus makes coacervation of colloid.Also there is physicochemical change simultaneously, in and the electric charge on colloidal particle and suspended substance surface, reduce δ current potential, colloidal particle are made to become attracting from original repelling each other, destroy micelle stability, colloidal particle are collided mutually, thus form cotton-shaped coagulating sedimentation, the surface-area of precipitation can reach (200 ~ 1000) m2/g, has adsorptive power.
In recent years, along with the development of nanotechnology, increasing nano material is produced, simultaneously the new feature that has of nano material also studied personnel find.
Nano-particle material is also called ultrafine particle material, is made up of nanoparticle (nano particle).Nanoparticle is also ultrafine particle, generally refer to the particle of size between 1 ~ 100nm, it is the transitional region being in cluster and macro object boundary, from common about microcosmic and macroscopical viewpoint, such system is atypical microscopic system also atypical macrosystem both, be a kind of typical Mesoscopic structure, it has surface effects, small-size effect and macro quanta tunnel effect.After macro object is subdivided into ultrafine particle (nano level) by people, it will demonstrate many unusual characteristics, and namely the character of its optics, calorifics, electricity, magnetics, mechanics and chemical aspect will have significant difference with comparing during bulk solid.
And at present the biological flocculation of indivedual nano material has also been in the news, such as Nano chitosan can flocculation microalgae, and the mixture of chitosan-nano metal also can flocculation microalgae.But, utilize the research of nano material flocculating microbial to rarely have report at present.
In current production, the flocculation of microorganism mainly adopts centrifugal method, and the method is higher to instrument requirements, needs to consume mass energy simultaneously.A kind of method finding simple and efficient flocculating microbial is produced significant to microbiological industry from now on.And nano material was widely used in the fields such as engineering manufacture, medical science in recent years owing to having some properties.We find that nano aluminium oxide can significantly flocculate as flocculation agent the fermentation thalli of the microorganisms such as subtilis, the use of this flocculation agent can replace conventional centrifugal method, be applicable to the flocculation precipitation pretreatment of bacterium class fermented liquid, there is huge application value.
Summary of the invention
The object of the present invention is to provide a kind of throwing out that can significantly improve thalline, thus conventional centrifugal method can be replaced, be specially adapted to the novel flocculant to the flocculation precipitation pretreatment of bacterium class fermented liquid and application thereof.
Object of the present invention can be achieved through the following technical solutions:
A kind of novel flocculant, is characterized in that the aqueous solution for nano aluminium oxide.
The aqueous solution of nano aluminium oxide of the present invention is the nano-solution of 10%.
The invention allows for a kind of method utilizing above-mentioned novel flocculant to obtain fermentation thalli or supernatant liquor, it is characterized in that comprising the following steps:
Step 1: to inoculation of medium bacterial classification, obtain fermentation thalli after fermenting;
Step 2: add above-mentioned novel flocculant in fermented liquid, adding concentration is 0.1mmol/L-20 mmol/L, mixes latter standing 30 minutes, the fermentation thalli in separating mixture and supernatant liquor.
In step 2 of the present invention, the interpolation concentration of novel flocculant is preferably 0.1 or 0.5 or 1 or 2 or 4 or 10 or 20mmol/L.
The invention allows for a kind of method utilizing above-mentioned novel flocculant to obtain fermentation thalli or supernatant liquor in sea bacillus subtilis fermented liquid, it is characterized in that comprising the following steps:
Step 1: to Landy inoculation of medium sea bacillus subtilis liquid spawn, temperature 28 ~ 30 DEG C, under rotating speed 120 ~ 150 r/min condition, ferment, fermentation time is 4-40 hour, to obtain fermentation thalli, the formula of described Landy substratum is: glucose 20 grams, Pidolidone 5g, yeast extract 1 gram, potassium primary phosphate 1 gram, magnesium sulfate heptahydrate 0.5 gram, 0.5 gram, Repone K, manganous sulfate 5 milligrams, cupric sulfate pentahydrate 0.16 milligram, 0.15 milligram, ferric sulfate, be settled to 1 liter with water, regulate pH to 7.0,115 DEG C of sterilizings 25 ~ 40 minutes;
Step 2: add nano aluminium oxide 0.1 ~ 20 mmol/L in fermented liquid, rock fermented liquid, latter static 30 minutes, is separated mixing solutions, obtains fermentation thalli and supernatant liquor in sea bacillus subtilis fermented liquid.
Beneficial effect of the present invention: adopt nano material, be a kind of inorganic, metal oxide, have nontoxic, flocculation efficiency is high, and consumption is few, conveniently advantage, can replace conventional centrifugal method, be applicable to the flocculation precipitation pretreatment of bacterium class fermented liquid, there is huge application value.
accompanying drawing illustrates:
Accompanying drawing 1 is flocculation treatment schematic diagram in the embodiment of the present invention 1.
Accompanying drawing 2 is required nano aluminium oxide consumption graphic representations of growth curve flocculation of each stage in the embodiment of the present invention 1.
Accompanying drawing 3 is Flocculation Results graphic representations under different PH condition in the embodiment of the present invention 2.
embodiment:
Below in conjunction with example, the present invention is described in further details:
The present invention proposes a kind of novel flocculant, it is characterized in that the aqueous solution for nano aluminium oxide, the aqueous solution of described nano aluminium oxide is the nano-solution of 10%;
The invention allows for a kind of method utilizing above-mentioned novel flocculant to obtain fermentation thalli or supernatant liquor, it is characterized in that comprising the following steps:
Step 1: to inoculation of medium bacterial classification, bacterial classification can be intestinal bacteria, guard against bacillus amyloliquefaciens or subtilis, obtains fermentation thalli after fermenting;
Step 2: add above-mentioned novel flocculant in fermented liquid, adding concentration is 0.1mmol/L-20 mmol/L, mixes latter standing 30 minutes, the fermentation thalli in separating mixture and supernatant liquor.
embodiment one
1. configure Landy substratum, filling a prescription is: glucose 20 grams, Pidolidone 5g, yeast extract 1 gram, potassium primary phosphate 1 gram, magnesium sulfate heptahydrate 0.5 gram, 0.5 gram, Repone K, manganous sulfate 5 milligrams, cupric sulfate pentahydrate 0.16 milligram, 0.15 milligram, ferric sulfate.Be settled to 1 liter with water, regulate pH to 7.0.115 DEG C of sterilizings 25 ~ 40 minutes.
2. in the Landy substratum configured, access Bacillus subtilis strain with the inoculum size of 2 %, temperature 28 DEG C, under rotating speed 150 r/min condition, carry out the fermentation of first stage, fermentation time is respectively 4, and 6,8,12,18,24,36,40 hours.
3. respectively after fermentation ends, aseptically respectively to adding certain density nano aluminium oxide in fermented liquid, leaving standstill 30 minutes, measuring supernatant liquor light absorption value (OD600), and adding up the consumption of relevant nanometer aluminum oxide.(Fig. 1, Fig. 2)
embodiment two
1. configure Landy substratum, filling a prescription is: glucose 20 grams, Pidolidone 5g, yeast extract 1 gram, potassium primary phosphate 1 gram, magnesium sulfate heptahydrate 0.5 gram, 0.5 gram, Repone K, manganous sulfate 5 milligrams, cupric sulfate pentahydrate 0.16 milligram, 0.15 milligram, ferric sulfate.Be settled to 1 liter with water, regulate pH to 7.0.115 DEG C of sterilizings 25 ~ 40 minutes.
2. in the Landy substratum configured, access Bacillus subtilis strain with the inoculum size of 2 %, temperature 28 DEG C, under rotating speed 150 r/min condition, ferment, fermentation time is respectively 24 hours (OD600=1.0).
3., when fermentation ends, in fermented liquid, add 1 M HCl or 1 M NaOH regulate pH to 5.0,7.0,9.0.
4. aseptically add respectively in different pH fermented liquid the nano aluminium oxide that final concentration is 10 mM, leave standstill 30 minutes.Measure supernatant liquor absorbancy, analyze flocculating effect.(Fig. 3)
comparative example:
According to the method preparation Landy substratum in embodiment 1, if three repetitions.115 DEG C of sterilizings 30 minutes.With 2 % inoculum size access Bacillus subtilis strains, 28 DEG C, 150 r/min cultivate 30 hours.The sterilized water adding respective volume after fermentation ends contrasts.
The present invention, relative to prior art, adopts nano level inorganic, metal oxide, has nontoxic, flocculation efficiency is high, and consumption is few, conveniently advantage, can replace conventional centrifugal method, be applicable to the flocculation precipitation pretreatment of bacterium class fermented liquid, there is huge application value.

Claims (5)

1. a novel flocculant, is characterized in that the aqueous solution for nano aluminium oxide.
2. a kind of novel flocculant according to claim 1, is characterized in that the aqueous solution of described nano aluminium oxide is the nano-solution of 10%.
3. utilize as in claim 1-2, novel flocculant as described in any one obtains a method for fermentation thalli or supernatant liquor, it is characterized in that comprising the following steps:
Step 1: to inoculation of medium bacterial classification, obtain fermentation thalli after fermenting;
Step 2: add above-mentioned novel flocculant in fermented liquid, adding concentration is 0.1mmol/L-20 mmol/L, mixes latter standing 30 minutes, separating mixture, obtains fermentation thalli and supernatant liquor.
4. a kind of method utilizing novel flocculant to obtain fermentation thalli or supernatant liquor according to claim 3, is characterized in that the interpolation concentration of novel flocculant in step 2 is preferably 0.1 or 0.5 or 1 or 2 or 4 or 10 or 20mmol/L.
5. utilize as in claim 1-2, novel flocculant as described in any one obtains a method for fermentation thalli or supernatant liquor in sea bacillus subtilis fermented liquid, it is characterized in that comprising the following steps:
Step 1: to Landy inoculation of medium sea bacillus subtilis liquid spawn, temperature 28 ~ 30 DEG C, under rotating speed 120 ~ 150 r/min condition, ferment, fermentation time is 4-40 hour, to obtain fermentation thalli, the formula of described Landy substratum is: glucose 20 grams, Pidolidone 5g, yeast extract 1 gram, potassium primary phosphate 1 gram, magnesium sulfate heptahydrate 0.5 gram, 0.5 gram, Repone K, manganous sulfate 5 milligrams, cupric sulfate pentahydrate 0.16 milligram, 0.15 milligram, ferric sulfate, be settled to 1 liter with water, regulate pH to 7.0,115 DEG C of sterilizings 25 ~ 40 minutes;
Step 2: add nano aluminium oxide 0.1 ~ 20 mmol/L in fermented liquid, rock fermented liquid, latter static 30 minutes, is separated mixing solutions, obtains fermentation thalli and supernatant liquor in sea bacillus subtilis fermented liquid.
CN201510118484.4A 2015-03-18 2015-03-18 A kind of novel flocculant and its application Active CN104709988B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109337944A (en) * 2018-10-30 2019-02-15 浙江海洋大学 A method of flocculant is prepared using bacillus marinus
CN112655462A (en) * 2020-12-18 2021-04-16 廊坊师范学院 Mushroom reduction strain and preparation and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013059754A1 (en) * 2011-10-20 2013-04-25 Board Of Regents, The University Of Texas System Continuous flocculation deflocculation process for efficient harvesting of microalgae from aqueous solutions
CN103265104A (en) * 2013-03-14 2013-08-28 苏州旭达环保科技有限公司 Nano powder-organic polymer composite flocculating agent

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013059754A1 (en) * 2011-10-20 2013-04-25 Board Of Regents, The University Of Texas System Continuous flocculation deflocculation process for efficient harvesting of microalgae from aqueous solutions
CN103265104A (en) * 2013-03-14 2013-08-28 苏州旭达环保科技有限公司 Nano powder-organic polymer composite flocculating agent

Non-Patent Citations (2)

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Title
厉月含: "纳米Al2O3在海水中的沉降及其对磷和腐殖酸的吸附研究", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 *
马偲: "水质纳米颗粒的自团聚及与藻细胞的异团聚", 《中国优秀硕士学位论文全文数据库 基础科学辑》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109337944A (en) * 2018-10-30 2019-02-15 浙江海洋大学 A method of flocculant is prepared using bacillus marinus
CN112655462A (en) * 2020-12-18 2021-04-16 廊坊师范学院 Mushroom reduction strain and preparation and application thereof

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