CN103435250B - A kind ofly add the method that microbial flocculant improves activated sludge dehydration performance - Google Patents
A kind ofly add the method that microbial flocculant improves activated sludge dehydration performance Download PDFInfo
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Abstract
The present invention patent discloses and a kind ofly adds the method that microbial flocculant improves activated sludge dehydration performance, separation from sludge sewage, purifying, screen to produce the klebsiella (Klebsiella of microbial flocculant? pneumoniae), temperature is 25 ~ 40 DEG C, rotating speed is 100 ~ 200r/min oscillation and fermentation cultivation, 2 ~ 3d, takes frozen ethanol liberation method to extract flocculant sample from fermented supernatant fluid.In activated sludge sample, add microbial flocculant, the sludge specific resistance (SRF) after conditioning and water ratio are reduced to 4.66 × 10 respectively
12m/kg and 83.74%, dehydrating effect is better than the chemical floc such as Tai-Ace S 150, polymerize aluminum chloride (PAC).The characteristic adaptation such as microbial flocculant is useless to mud salt, pH value are good, and after conditioning, the dewatering of active sludge significantly improves, and has the advantage such as readily biodegradable, high effect nontoxic.
Description
Technical field
The present invention relates to and a kind ofly add the method that microbial flocculant improves activated sludge dehydration performance, be absorbed in a kind of separation of flcos producing bacteria, purifying and screening, and the application in activated sludge dehydration, belong to environmental engineering and technical field of sludge treatment.
Background technology
Active sludge is the byproduct of municipal wastewater process, and with the expansion of city and Industrial Waste Water Treatments scale, excess sludge production increases year by year.Within 2010, China's Sewage Plant produces mud about 2,170 ten thousand t (in water ratio 80%), and the harmlessness disposing of a large amount of mud becomes a great problem of field of Environment Protection day by day.Excess sludge water ratio is high, and dewatering is poor, containing a large amount of hardly degraded organic substance, heavy metal, and a small amount of pathogenic micro-organism and parasitic ovum etc., usually do not reach the requirement of follow-up landfill, incineration disposal, cause mud transportation cost and dispose difficulty too high.
Higher water ratio is the main restricting factor of restriction sludge reduction and resource utilization, takes physics, chemistry or biological method to nurse one's health mud to improve dehydrating effect, is the effective way reducing sludge volume, reduce disposal and recycling cost.At present existing electrolysis, acid treatment, ultrasonic, microwave, the cold physical means such as to melt are applied to sludge dewatering, but physics dewatering energy consumption, expense are too high, limit its large-scale promotion in water treatment field.Chemical oxidation techniques is also applied to the dehydrating effect improving mud gradually, such as, by Fe
2+and H
2o
2the strong oxidizing solution Fenton reagent of composition, being applied to conditioning mud can effective degradation of organic substances, dissolves and destruction EPS structure, release cells internal water.
Add chemical amendment (such as, aluminium salt, polyacrylamide, tensio-active agent etc.) conditioning mud, also can change the flocculation structure of mud, reach the object of the dewatering improving mud.Sludge Chemical Conditioning is simple to operate, effect stability, is widely used in actual sewage process practice.Wherein, cationic polyacrylamide (CPAM) is most popular a kind of chemotype flocculation agent, but CPAM produces the intermediate product of difficult degradation, and has neurotoxicity and carinogenicity, can cause the secondary pollution of water body; Inorganic flocculating agent such as polymerize aluminum chloride (PAC) etc. can cause aluminum concentration in water to raise, and bring out the diseases such as senile dementia, environmental health risk rises.
Microbial flocculant (Microbialflocculants, MBF) refers to the natural high molecular substance with flocculation activity secreted in microbial metabolism, comprises the compositions such as acidic polysaccharose, protein, fat and nucleic acid.Relatively traditional organic and inorganic flocculating agent, microbial flocculant, owing to having the advantages such as with low cost, highly effective and safe, becomes the environmentally friendly water conditioner being subject to common concern in recent years.Many research analyzes chemical means by spectrum, chromatogram etc., the purifying of microorganisms flocculation agent, functional group and component qualification, and supposition is made to its flocculation mechanism, or be characterized as point of penetration with flocculation agent fermentation kinetics, inquire into microbial flocculant and produce and activity optimization.Microbial flocculant is at heavy metal wastewater thereby, and waste water fermentation, the application of low-temperature wastewater, dyeing waste water etc. all reaches good treatment effect, but relates to less in sludge dewatering improving, and rarely has klebsiella producing microbial flocculation agent to nurse one's health the report of mud.
Existing patent CN101367597A discloses a kind of flocculation agent for sludge dewatering and mud dewatering method, the flocculation agent related to is that polymerize aluminum chloride (PAC) and polyacrylamide (PAM) are composited, for typical inorganic-organic composite flocculating agent, be applied to production cost when nursing one's health sludge dewatering high, the problems such as the secondary pollution brought during traditional flocculant cannot be avoided again to apply and health risk.Microbial flocculant and organic polymer coargulator CPAM similar, chain-like structure can effectively promote build bridge formation, active group (-OH-,-COO-,-CONH on molecule
2-,-NH-etc.) for suspended particle provides adsorption site, destroy the dispersed texture of mud colloid, particle aggregation is also incorporated into comparatively big flocculating body.In addition, microbial flocculant is by Van der Waals force and hydrogen bond action, and compression colloid surface electrostatic double layer, under the combined action of adsorption bridging and compression double electric layer, reaches the object improving dehydrating residual active sludge effect.
Active sludge water ratio is high, and salinity is high, and microflora's composition is complicated.Mud salinity is closely related with lipid content in born of the same parents to microorganism growth, the activity of remote effect microbial flocculant, remarkable on the impact of sludge dewatering effect.Publication (CN101225405A) relates to a kind of production and using method of microbial flocculant, with starch wastewater and compound nitrogen source culturing micro-organisms, selected bacterial classification is that after purifying, optical density(OD) is the Klebsiella seed liquor of 1.5 ~ 1.8, and the microbial flocculant of generation is used for high-turbidity water process.This patent does not relate to the application of klebsiella produce flocculant in sludge dewatering, and the bacterial strain after direct enlarged culturing purifying can by the impact of the characteristics such as mud salinity, and its flocculation activity receives restriction to a certain degree.The strain separating that the present invention relates to, to screen from sewage work's concentration basin, and the microbial flocculant produced that fermented is used for this sewage plant sludge process, good to mud environmental compatibility, ensure better to improve sludge dewatering effect.
Summary of the invention
1. goal of the invention
Patent of the present invention is for city and Industrial Waste Water Treatments plant excess sludge dewatering is poor, mud cake subsequent disposal cost of disposal is higher present situation, overcome the environmental risk that conventional inorganic and organic polymer coargulator cause in the application, a kind of method adding microbial flocculant conditioning mud is provided, to improve the dewatering of residual active sludge, while avoiding secondary pollution problems, reach the object realizing sludge reduction, disposal of resources.
2. technical scheme
Patent of the present invention provides a kind of and adds the method that microbial flocculant improves activated sludge dehydration performance, and main technical scheme is as follows:
(1) separation and purification of sludge microbe
Mud sample dilution 10
2~ 10
8different concns gradient after, by spread plate method, sludge microbe is inoculated in nutrient agar.In 25 ~ 30 DEG C of constant incubators, cultivate 2 ~ 3d after inoculation, plate culture medium is separated and obtains multiple-microorganism bacterium colony.Plate streak picking colony carries out enlarged culturing in culture dish.Repeat above-mentioned steps, after 3 ~ 5 separation and purification, obtain the microorganism of different colonial morphology, be inoculated on slant medium, in 4 DEG C of refrigerators storages.
(2) screening of bacterium for producing flocculant
In inoculation liquid medium within after separation and purification, postvaccinal nutrient solution is at 25 ~ 40 DEG C of constant-temperature table shaking culture 2 ~ 3d, and at the centrifugal 15 ~ 30min of 4000 ~ 5000r/min to remove cell paste, supernatant liquor is liquid microbe flocculation agent.With fermented supernatant fluid to the flocculating rate (Fr) of 2 ~ 5g/L aqueous suspension ofkaolin for index, the high reactivity bacterium for producing flocculant screening stable.
(3) extraction of microbial flocculant
Get the produce flocculant bacterial strain after purifying, screening from inclined tube substratum, be seeded to the Erlenmeyer flask that 50mL liquid nutrient medium is housed, be placed in 25 ~ 40 DEG C of constant-temperature table fermentations, oscillation and fermentation cultivation 2 ~ 3d under the rotating speed of 100 ~ 200r/min.Fermented liquid is at the centrifugal 30min of 4000 ~ 5000r/min, and supernatant liquor adds the freezing dehydrated alcohol of 2 ~ 4 times of volumes after revolving steaming, in 4 DEG C of refrigerators, leave standstill 12 ~ 24h.The centrifugal 30min of 4000 ~ 5000r/min, precipitation crude product is dissolved in deionized water, add the cetylpyridinium chloride (CPC) of 2%, the composite precipitation of microbial flocculant and cetyl pyridinium is dissolved in the NaCl solution of 0.5mol/L again, add the dehydrated alcohol of the precooling of 2 ~ 4 times of volumes, use washing with acetone 2 ~ 3 times after Precipitation, lyophilize obtains the microbial flocculant solid sample after purifying.
(4) microbial flocculant conditioning sludge dewatering is added
Above-mentioned steps directly from mud sample purifying, screening obtain klebsiella, investigate it when being applied in opsonic activity mud, improving the effect in dewatering performance of sludge.After activated sludge sample adds microbial flocculant, stir 30 ~ 60s soon at magnetic stirring apparatus 400 ~ 500r/min, 100 ~ 200r/min stirs 3 ~ 6min slowly, moves to sludge specific resistance instrument, constant voltage suction filtration under the vacuum degree condition of 0.04 ~ 0.2MPa.
3. novelty of the present invention
What patent of the present invention related to a kind ofly adds the method that microbial flocculant improves activated sludge dehydration performance, and compared with existing excess sludge dewatering, technique, novelty is as follows:
(1) the present invention is directly from Sewage Plant active sludge screening bacterium for producing flocculant of microbe; produce flocculant bacterium source is extensive; in 25 ~ 30 DEG C of constant incubators, 2 ~ 3d is cultivated after the inoculation of spread plate method; repeat purifying 3 ~ 5 times i.e. separable, bacterial strain that purifying is dissimilar in a large number; strain growth metabolism is fast; the cycle of separation and purification is short, easy handling, can adapt to large-scale production.
(2) with bacterial strain to the flocculating rate (Fr) of aqueous suspension ofkaolin for index, from the bacterial strain after above-mentioned steps purifying, the high reactivity MBF producing strains of screening to the flocculating rate of aqueous suspension ofkaolin more than 90%.The flocculation dewatering result of aqueous suspension ofkaolin and mud keeps high consistency, ensures the bacterial strain of screening improvement effect to dehydration when being applied to sludge conditioning.
(3) when flocculating rate Fr measures, and non-selected deionized water but choose fresh culture for blank, effectively get rid of the throwing out of liquid nutrient medium own to the interference of MBF flocculation activity, reflect the activity of microbial flocculant more accurately.
(4) screening the flcos producing bacteria obtained is 25 ~ 40 DEG C in temperature, oscillation and fermentation cultivation 2 ~ 3d under the rotating speed of 100 ~ 200r/min, microbial flocculant culture temperature is normal temperature condition, rotating speed and energy consumption lower and be easy to realize, the production cost of relative chemical flocculation agent reduces greatly.
(5) bacterium for producing flocculant after purifying keeps good flocculation stability to pH value, temperature etc., pH value be in 4 ~ 8 scopes flocculation activity more than 80%, more than 60% is remained in the scope that temperature is 20 ~ 60 DEG C, the tolerance that condition is as good in performances such as sludge pH loads to external world, microbial flocculant is with a wide range of applications.
(6) the microbial flocculant sample extraction that this patent relates to takes frozen ethanol liberation method, simple and easy to do and consuming time shorter, and flocculation agent output is high, and 1000mL fermented supernatant fluid can extract the flocculation agent crude product of about 1.68g.
(7), after adding klebsiella flocculation agent opsonic activity mud, sludge specific resistance (SRF) and water ratio are reduced to 4.66 × 10 respectively
12m/kg and 83.74%, dewatering performance of sludge significantly improves, and the dehydrating effect of microbial flocculant is better than inorganic flocculating agent Tai-Ace S 150, polymerize aluminum chloride (PAC) etc., and dehydrated sludge transport and processing costs reduce.
(8) microbial flocculant (MBF) that klebsiella is produced is applied to activated sludge dehydration by this patent first, and to sludge dewatering effect, there is remarkable improvement result, this mud dewatering method investment running cost is cheap, can be excess sludge process disposal and provides new thinking.。
(9) the product flocculated bacteria screened is good to adaptability such as mud salinity, pH value, ensures better to improve sludge dewatering effect.And there is the outstanding advantages such as readily biodegradable, high effect nontoxic, can avoid the secondary pollution problems of chemical floc, be the new and effective flocculation agent had broad application prospects.
Accompanying drawing explanation
Accompanying drawing 1 is scanning electron microscope (SEM) form of bacterium for producing flocculant strain.
Accompanying drawing 2 is pH and the thermostability of microbial flocculant.
Accompanying drawing 3 is sludge specific resistance measurement device schematic diagram.
Accompanying drawing 4 is the impact of microbial flocculant (MBF) conditioning on sludge dewatering effect.
Accompanying drawing 5 is the sludge dewatering effectiveness comparison of microbial flocculant (MBF) and conventional flocculant.
Embodiment
A kind of microbial flocculant that adds of the present invention is to improve the method for dehydrating residual active sludge performance, and when concrete enforcement runs, take from Yangtze River Delta Area city sewage plant concentration basin for examination mud sample, this Sewage Plant adopts A
2/ O denitrogenation dephosphorizing treatment process, design discharge is 100,000 t/d, and actual water inlet is 60,000 t/d, residue waste sludge output 40 ~ 45t/d.Waste water is mainly sanitary sewage (70% ~ 80%), trade effluent mainly Electronics Factory, Tong Xin Electroplate Factory, hair long battery production factory produce waste water, concentration basin dewatering technology of mud: after adding polyacrylamide (PAM) conditioning, carry out belt type filter-pressing dehydration plant.For examination sludge based eigen as table 1.
Table 1 is for examination mud sample essential characteristic
| pH | Water ratio (%) | Sludge specific resistance (× 10 12m/kg) | Mud particle diameter (μm) |
| 7.51 | 98.86 | 11.64 | 53.42 |
Mud sample, on aseptic operating platform, dilutes different concns gradient (10 successively
2~ 10
8), spread plate method is by microbial inoculant in mud on 1 and the dissimilar nutrient agar of II, and wherein substratum I forms: KCl0.5g/L, NaNO
32g/L, FeSO
40.01g/L, K
2hPO
41g/L, MgSO
40.5g/L, sucrose 30g/L, agar 20g/L; Medium ii forms: extractum carnis 3g/L, peptone 10g/L, NaCl5g/L, agar 20g/L, 120 DEG C of high pressure steam sterilization 30min.After inoculation, culture dish is inverted, and at 28 DEG C of biochemical cultivation case constant temperature culture 48h, plate culture medium is separated and obtains multiple-microorganism bacterium colony.Plate streak picking colony, in glass culture dish, repeats above-mentioned steps 4 times, obtains the microorganism strains after different shape separation and purification, be inoculated on slant medium, in 4 DEG C of refrigerator storages.
Liquid nutrient medium I forms: KCl0.5g/L, NaNO
32g/L, FeSO
40.01g/L, K
2hPO
41g/L, MgSO
40.5g/L, sucrose 30g/L; Liquid nutrient medium II forms: extractum carnis 3g/L, peptone 10g/L, NaCl5g/L, 120 DEG C of high pressure steam sterilization 30min.Picking purifying bacterial strain from slant medium, in inoculation liquid nutrient medium I and II after sterilization, 37 DEG C of constant-temperature table shaking culture 50h, the centrifugal 30min of 5000r/min, the flocculating rate (Fr) of supernatant liquor after centrifugal to 4g/L aqueous suspension ofkaolin is index, screening high reactivity bacterium for producing flocculant.
High reactivity flocculation agent is obtained by substratum I cultivation through above-mentioned separation, screening step, be 91.70% to the flocculation activity of aqueous suspension ofkaolin, bacterial strain is creamy white bacterium colony on nutrient agar, Gram-negative, catalase reaction is positive, and scanning electron microscope hypothallus form is in the rod-short (accompanying drawing 1) of rule.Through 16SrDNA gene order and strain morphology qualification, identify that bacterial strain is Klebsiella pneumoniae (Klebsiellapneumoniae), GenBank sequence number is CP000647, and homology degree is up to 99%.
The fermented liquid of klebsiella at the centrifugal 30min of 5000r/min to remove cell paste, supernatant liquor be evaporated to that volume is original volume on a rotary evaporator 1/4, add the freezing dehydrated alcohol of 2 times of volumes, 18h is left standstill in 4 DEG C of refrigerators, the centrifugal 30min of 5000r/min, after precipitation crude product is water-soluble, add the cetylpyridinium chloride of 2% while stirring, the composite precipitation of microbial flocculant and cetyl pyridinium is dissolved in the NaCl solution of 0.5mol/L again, add the dehydrated alcohol of the precooling of 3 times of volumes, washing with acetone 2 times are used after Precipitation, lyophilize obtains the microbial flocculant after purifying, every 1000mL fermented supernatant fluid can extract the flocculation agent crude product of about 1.68g through cold Ethanol Method.
Embodiment 1
Fermented liquid after shaking culture is at the centrifugal 30min of 5000r/min, and supernatant liquor is liquid microbe flocculation agent, the klebsiella produce flocculant be separated, screen is applied to process 4g/L kaolin suspension solution.Cross the 0.4g kaolin of 60 mesh sieves, the CaCl of 5mL
2(1%) solution and 2mL fermented supernatant fluid are mixed in 250mL beaker, add deionized water to 100mL, and magnetic stirring apparatus 400r/min stirs 1min soon, and 150r/min stirs 5min slowly, are transferred in 100mL graduated cylinder and leave standstill 5min.Under ultraviolet spectrophotometer 550nm wavelength, measure supernatant liquid absorbancy (A), choose fresh culture and replace fermented liquid, measure the aqueous suspension ofkaolin absorbancy (A in contrast after process
0) flocculating rate (Fr) calculation formula is as follows:
In formula, Fr-flocculating rate, %; The absorbancy of aqueous suspension ofkaolin after the process of A-fermented supernatant fluid; A
0the absorbancy of aqueous suspension ofkaolin after the process of-fresh culture.
The microbial flocculant that klebsiella is produced is remarkable to aqueous suspension ofkaolin flocculating effect, process Hou Gao ridge scholar's particle aggregation degree improves, suspension liquid turbidity reduces, and it is good to process kaolinic flocculation stability, pH be 4 ~ 8, temperature is all keep high flocculation activity (accompanying drawing 2) in the scope of 20 ~ 60 DEG C, the change of the condition such as sludge loading, pH value can be adapted to, active more than 82.77% in the scope of pH=4 ~ 8, and the flocculating rate the highest (Fr=91.70%) during pH=7.
Embodiment 2
Klebsiella flocculation agent is applied to conditioning mud, to improve the dewatering of mud.25mL mud sample to be measured adds 0 ~ 5mL microbial flocculant, 60s is stirred soon at magnetic stirring apparatus 400r/min, 150r/min stirs 5min slowly, move to sludge specific resistance measurement device (accompanying drawing 3), filter under the condition of constant vacuum 0.05MPa, record filtering time t and corresponding filtrate volume V.Choose sludge specific resistance (SRF) and water ratio sign sludge dewatering effect, water ratio adopts gravimetric determination.Sludge specific resistance (Specificresistanceinfiltration, when filtering under SRF) representing constant pressure, the resistance of unit mass mud in unit filtration area. sludge specific resistance is larger, and strainability is poorer, and sludge specific resistance calculates sees formula (2):
In formula, P is for filtering pressure, N/m
2; A is filtration area, m
2; μ is filtrate viscosity, Ns/m
2; C is the dry solids wt that unit volumes of filtrate retains on filtration medium, kg/m
3, 1/C=C
i/ (100-C
i)-C
f/ (100-C
f); C
ifor former mud water ratio, %; C
ffor moisture content of the cake after suction filtration, %; B for the slope of straight line shown in equation of filtration t/V=bV+a, t be filtration time, s; V is filtrate volume, m
3.
The klebsiella MBF adding different amount nurses one's health mud, significantly can improve its flocculation dewatering performance (accompanying drawing 4).Under 3mL dosage, sludge specific resistance is by 11.64 × 10 of former mud
12m/kg is reduced to 4.66 × 10 after conditioning
12m/kg, water ratio is reduced to 83.74% after conditioning by former mud 98.86%.The microbial flocculant (MBF) that accompanying drawing 5 is produced for klebsiella and the contrast of chemical floc in conditioning sludge dewatering, equivalent (1% is added in active sludge, w/v) Tai-Ace S 150, polymerize aluminum chloride (PAC), cationic-type polyacrylamide (CPAM).After MBF conditioning, sludge specific resistance is reduced to 4.66 × 10 after conditioning
12m/kg, resistivity reduced rate is up to 59.97%, Al
2(SO
4)
3nurse one's health sludge specific resistance with PAC and be respectively 6.04 × 10
12m/kg, 5.25 × 10
12m/kg, after microbial flocculant conditioning, sludge dewatering successful is better than inorganic flocculating agent Tai-Ace S 150 and polymerize aluminum chloride (PAC), close with organic floculant CPAM dehydrating effect.
Claims (4)
1. one kind adds the method that microbial flocculant improves activated sludge dehydration performance, it is characterized in that: be separated from sludge sewage, purifying, screen to obtain high-activity microorganism bacterium for producing flocculant, temperature is 25 ~ 40 DEG C, rotating speed is 100 ~ 200r/min oscillation and fermentation cultivation, 2 ~ 3d, fermented liquid is at the centrifugal 30min of 4000 ~ 5000r/min, frozen ethanol liberation method is taked to extract flocculant sample from fermented supernatant fluid, add microbial flocculant in activated sludge sample, magnetic stirring apparatus 400 ~ 500r/min stirs 30 ~ 60s soon, 100 ~ 200r/min stirs 3 ~ 6min slowly, dewatering performance of sludge after microbial flocculant conditioning significantly improves, separation, the purifying of described high-activity microorganism bacterium for producing flocculant are: directly by sludge sewage diluted sample to different concns gradient, be seeded to the microorganism in nutrient agar culture sludge, 2 ~ 3d is cultivated in 25 ~ 30 DEG C of constant incubators, repeat above-mentioned steps 3 ~ 5 times, separation repeatedly, purifying obtain the bacterial strain of different shape, be inoculated on slant medium, in 4 DEG C of refrigerator storages, the screening of described high-activity microorganism bacterium for producing flocculant is: with bacterial strain to the flocculating rate Fr of the aqueous suspension ofkaolin of 4g/L for index, the flocculation agent that this bacterial strain produces is 91.70% to the flocculation activity of aqueous suspension ofkaolin, pH value be in 4 ~ 8 scopes flocculation activity more than 80%, more than 60% is remained in the scope that temperature is 20 ~ 60 DEG C, bacterium colony is creamy white, Gram-negative, form is the rod-short of rule, Klebsiella pneumoniae (Klebsiellapneumoniae) is accredited as through 16SrDNA gene order and strain morphology, GenBank sequence number is CP000647, homology degree is up to 99%.
2. method as described in claim 1, it is characterized in that: when measuring the flocculation activity of microbial flocculant, and non-selected deionized water but choose fresh culture for blank, the throwing out of effective eliminating liquid nutrient medium own, to the interference of Flocculation Activity of Mircrobial Flocculants, reflects the activity of microbial flocculant more accurately.
3. method as described in claim 1, it is characterized in that: described frozen ethanol liberation method is: fermented liquid is at the centrifugal 30min of 4000 ~ 5000r/min, supernatant liquor adds the freezing dehydrated alcohol of 2 ~ 4 times of volumes after revolving steaming, 12 ~ 24h is left standstill in 4 DEG C of refrigerators, the centrifugal 30min of 4000 ~ 5000r/min, precipitation crude product is dissolved in deionized water, add the cetylpyridinium chloride of 2%, the composite precipitation obtained is dissolved in the NaCl solution of 0.5mol/L again, add the dehydrated alcohol of the precooling of 2 ~ 4 times of volumes, washing with acetone 2 ~ 3 times are used after Precipitation, lyophilize obtains the microbial flocculant solid sample after purifying, frozen ethanol liberation method is simple and easy to do and consuming time shorter, flocculation agent output is high, 1000mL Klebsiella pneumoniae fermented supernatant fluid can extract the flocculation agent crude product of 1.68g.
4. method as described in claim 1, it is characterized in that: after activated sludge sample adds microbial flocculant, 30 ~ 60s is stirred soon at magnetic stirring apparatus 400 ~ 500r/min, 100 ~ 200r/min stirs 3 ~ 6min slowly, move to sludge specific resistance instrument, constant voltage suction filtration under the vacuum degree condition of 0.04 ~ 0.2MPa, sludge specific resistance and water ratio are respectively by 11.64 × 10 of former mud
12m/kg and 98.86%, is reduced to 4.66 × 10 after microbial flocculant conditioning
12m/kg and 83.74%, dewatering performance of sludge significantly improves, and the dehydrating effect of microbial flocculant is better than inorganic flocculating agent Tai-Ace S 150, polymerize aluminum chloride, and dehydrated sludge transport and processing costs reduce.
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| CN105176877B (en) * | 2015-10-12 | 2017-05-17 | 青岛耀东生物工程有限公司 | Klebsiella and method for preparing microbial flocculant by using same |
| CN106316029A (en) * | 2016-11-03 | 2017-01-11 | 中国地质大学（武汉） | Sludge conditioning and gas collecting integrated device and sludge conditioning and gas collecting method |
| CN107794238B (en) * | 2017-11-13 | 2020-10-13 | 中国科学院成都生物研究所 | BFX-01 strain for high yield of biological flocculant and biological flocculant obtained thereby |
| CA3097400A1 (en) * | 2018-04-20 | 2019-10-24 | Advanced Biological Services, Inc. | Systems and methods for treating wastewater and providing class a sludge |
| CN109293217A (en) * | 2018-11-19 | 2019-02-01 | 广州珞珈环境技术有限公司 | A kind of conditioner and its preparation method and application for sludge dewatering |
| CN109368960A (en) * | 2018-12-07 | 2019-02-22 | 珠海市清宇环保科技有限公司 | A kind of formula and its technique suitable for sludge dewatering and the complex microorganism medicament of heavy metals immobilization |
| CN112198302B (en) * | 2020-10-25 | 2022-12-16 | 中铁二局第一工程有限公司 | Method and measuring tool for rapidly detecting mud content of fine aggregate |
| CN112607988B (en) * | 2020-12-31 | 2022-10-04 | 广西壮族自治区环境保护科学研究院 | Biological regulator for reducing water content of sludge and application thereof |
| CN112645500B (en) * | 2020-12-31 | 2023-04-25 | 广西壮族自治区环境保护科学研究院 | Comprehensive sewage treatment method using biological regulator |
| CN113880395B (en) * | 2021-08-31 | 2023-10-13 | 芜湖海创环保科技有限责任公司 | Sludge dewatering complexing agent and sludge dewatering method |
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