CN104193010A - Microbial composite flocculant - Google Patents
Microbial composite flocculant Download PDFInfo
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- CN104193010A CN104193010A CN201410420813.6A CN201410420813A CN104193010A CN 104193010 A CN104193010 A CN 104193010A CN 201410420813 A CN201410420813 A CN 201410420813A CN 104193010 A CN104193010 A CN 104193010A
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Abstract
The invention relates to a microbial composite flocculant which comprises the following raw materials in parts by weight: 5-10 parts of Rhdococcus erythropolis leavening, 70-120 parts of 2 mol/L CaCl2 solution and 3-10 parts of chitosan. The floccule producing culture medium (g/L) of the Rhdococcus erythropolis in the fermentation process comprises 10g of glucose, 2g of K2HPO4, 2g of KH2PO4, 0.2g of MgSO4.7H2O, 0.1g of CaCl2, 0.5g of urea and 0.5g of yeast extract. The pH value is 7.2-7.5, and the culture medium is sterilized at 120 DEG C for 30 minutes. The microbial composite flocculant has more obvious flocculation effect than any flocculant used independently; and under the synergistic actions of the composite microbial flocculant and chitosan, the microbial composite flocculant can completely satisfy the requirements for sewage treatment, and can eliminate or lower the hazard of other flocculants to human.
Description
Technical field
The present invention relates to a kind of microorganism composite flocculation agent, belong to sewage treatment area.
Background technology
In sanitary sewage, production and Industrial Wastewater Treatment, the effect that flocculation process is mainly being born the suspended substance removed in water and colloidalmaterial, further purified water, by adding a certain amount of flocculation agent, at electrically neutralization, adsorption bridging, net, catching volume sweeps etc. under effect and makes water-borne glue body material be in contact with one another collision and surely de-, be gathered into cotton-shaped alumen ustum, precipitation, clarification water quality under action of gravity.
Flocculation agent mainly comprises inorganic flocculating agent, organic floculant and microbial flocculant.Inorganic flocculating agent comprises Tai-Ace S 150, aluminum chloride, ferric sulfate, iron(ic) chloride etc., wherein Tai-Ace S 150 Shi You U.S. exploitation the earliest, and a kind of important inorganic flocculating agent of always using till today.Conventional aluminium salt has Tai-Ace S 150 AL
2(SO
4)
318H
2o and alum AL
2(SO
4)
3k
2sO
424H
2o, another kind of is that molysite has Iron trichloride hexahydrate FeCL
36H
2o. ferrous sulfate hydrate FeSO
417H
2o and ferric sulfate.Organic polymer coargulator has natural polymer and the large class of synthetic polymer two.Chemical structure, can be divided into following 3 types: (1) polyamine type: low molecular weight cationic type ionogen; (2) quaternary ammonium type: the change of molecular weight scope is large, and there is higher cationic; (3) multipolymer of acrylamide: molecular weight is higher, can necessarily all sell with the formulation of emulsus or powdery to millions of, several by hundreds of thousands of, more inconvenient in use, but flocculating property is good.In organic polymer coargulator macromole, can be with-COO-,-NH-,-SO
3,-the hydrophilic radicals such as OH, have the various structures such as chain, ring-type.The features such as because its active group is many, molecular weight is high, has consumption few, and scum silica frost output is few, and flocculation ability is strong, and flco is easily separated, and oil removing and oil removal are effective.
Yet, inorganic flocculating agent low price, but can have a negative impact to human health and ecotope; Although organic polymer coargulator consumption is few, scum silica frost output is few, and flocculation ability is strong, flco is easily separated, oil removing and oil removal are effective, but the residual monomer of this class superpolymer has " three cause " effect (causing rugged, carcinogenic, mutagenesis), thereby its range of application is restricted.
Microbial flocculant is that a class is by the macromolecule organic with flocculating function of microorganisms.Mainly contain glycoprotein, mucopolysaccharide, Mierocrystalline cellulose and nucleic acid etc.From its source, also belong to natural organic high-molecular flocculant, so it have all advantages of natural organic high-molecular flocculant.Simultaneously, the research work of microbial flocculant is entered into and is utilized biotechnology to cultivate, screen good bacterial classification by purification, modification, with lower cost, obtain the research of efficient flocculation agent, so its research range has surmounted the research category of traditional natural organic high-molecular flocculant.The microorganism with secretion flocculation agent ability is called bacterium for producing flocculant.
Summary of the invention
The shortcoming that the present invention is directed to inorganic flocculating agent and organic floculant in prior art, provides a kind of microorganism composite flocculation agent, and flocculation activity of the present invention is high, the feature of safety non-toxic, non-secondary pollution.
Technical scheme of the present invention is as follows:
A microorganism composite flocculation agent, comprises following raw material by weight: rhodococcus erythropolis fermented product 5-10 part, 2mol/L CaCl
2solution 70-120 part, chitosan 3-10 part.Preferably, rhodococcus erythropolis fermented product 6-8 part, 2mol/L CaCl
2solution 80-110 part, chitosan 4-7 part.Preferred, 7 parts of rhodococcus erythropolis fermented products, 2mol/L CaCl
290 parts of solution, 5 parts of chitosans.
The product wadding substratum (g/L) using in above-mentioned rhodococcus erythropolis fermenting process: glucose 10g, K
2hPO
42g, KH
2pO
42g, MgSO
47H
2o 0.2g, CaCl
20.1g, urea 0.5g, yeast extract paste 0.5g, pH7.2-7.5,120 ℃ of sterilizing 30min.
In fermenting process of the present invention, inoculum size is 3%-5%, and culture temperature is 28-35 ℃, and air flow is 1.5m
3/ h.Preferably, inoculum size is 4%, and culture temperature is 30 ℃.
The input amount of flocculation agent is 90-130mL/L, and preferred, input amount is 100 mL/L.
In the present invention, rhodococcus erythropolis is before inoculation fermentation, need carry out bacterial strain rejuvenation, be about to-70 ℃ of preservations to carry out rejuvenation with the bacterial classifications of having degenerated 4 ℃ of preservations, bacterial classification is carried out on minimum medium flat board 2-3 time to the single bacterium colony of the separated acquisition of line again, a plurality of single bacterium colonies are carried out respectively to inclined-plane line, then from inclined-plane, connect bacterium and produce in wadding substratum and carry out fermentation culture to liquid.
Microbial flocculant belongs to natural biological polymer, and structure is more complicated.From chemical constitution, be mainly various polysaccharides, the protein producing in microbial metabolism or have carbohydrate and the macromolecular compound of Protein formation, wherein a kind of sugar monomer of some origin of an incident of saccharan is polymerized, and some is to be polymerized by multiple sugar monomer.The microbial flocculant also having contains Ca
2+, Mg
2+, Fe
3+, Al
3+deng metallic cation.
The kind of microbial flocculant: (1) directly utilizes microbial cells as flocculation agent, as some bacterium, actinomycetes, yeast and mould; (2) flocculation agent extracting from microorganism wall.As the chitosan of the dextran of yeast cell wall, filamentous fungal cells wall has stronger throwing out; (3) utilize microbial metabolites to make flocculation agent.As the pod membrane of bacterium and lymphatic temperament, main component is polysaccharide and a small amount of polypeptide, protein, lipid and mixture thereof.What wherein can be used as flocculation agent is mainly polysaccharose substance; (4) flocculation agent that utilizes gene clone technology to obtain, is to utilize genetic engineering technique, and efficient flocculating transgenosis, in the thalline of being convenient to ferment, is constructed to Efficient Genetic bacterial strain, thus the microbial flocculant that production department comes.
Affect the factor that microbial flocculant produces: mainly comprise the kind, medium component, culture condition of bacterium for producing flocculant etc.The kind of microorganism is different, and the ability of its produce flocculant is also different.The carbon-nitrogen ratio of substratum, somatomedin etc. all have influence on the generation of flocculation agent.Generally containing monose, nutritious substratum, be conducive to the generation of flocculation agent.The pH of the required fermented liquid of different microorganisms produce flocculant is also different, and the best pH of conventionally grow best pH and produce flocculant is also inconsistent.The optimum temps of general produce flocculant is between 25-35 ℃, and temperature is too low can affect thalli growth, and excess Temperature can make the activity decreased of produced flocculation agent.Excessive or the how little output that all can affect thalli growth and flocculation agent of air flow, and require also different at the different air flow in vegetative period of microorganism.
Wherein a kind of flocculation agent effect is remarkable than independent use for the flocculating effect that microorganism composite flocculation agent of the present invention produces, by the synergy of compound microbial flocculation agent and chitosan, the requirement of sewage disposal can be met completely, use due to other flocculation agents harm to the mankind can be eliminated or reduce.
Embodiment
Below in conjunction with specific embodiment, further describe the present invention, advantage and disadvantage of the present invention will be more clear along with description.But embodiment is only exemplary, scope of the present invention is not formed to any restriction.It will be understood by those skilled in the art that lower without departing from the spirit and scope of the present invention and can the details of technical solution of the present invention and form be modified or be replaced, but these modifications and replacement all fall within the scope of protection of the present invention.
Embodiment 1
A microorganism composite flocculation agent, comprises following raw material by weight: 5 parts of rhodococcus erythropolis fermented products, 2mol/L CaCl
272 parts of solution, 3 parts of chitosans.The product wadding substratum (g/L) using in rhodococcus erythropolis fermenting process: glucose 10g, K
2hPO
42g, KH
2pO
42g, MgSO
47H
2o 0.2g, CaCl
20.1g, urea 0.5g, yeast extract paste 0.5g, pH7.4,120 ℃ of sterilizing 30min.
In fermenting process, inoculum size is 3%, and culture temperature is 30 ℃, and air flow is 1.5m
3/ h.
The input amount of microorganism composite flocculation agent of the present invention is 95mL/L.
Embodiment 2
A microorganism composite flocculation agent, comprises following raw material by weight: 8 parts of rhodococcus erythropolis fermented products, 2mol/L CaCl
2110 parts of solution, 7 parts of chitosans.The product wadding substratum (g/L) using in rhodococcus erythropolis fermenting process: glucose 10g, K
2hPO
42g, KH
2pO
42g, MgSO
47H
2o 0.2g, CaCl
20.1g, urea 0.5g, yeast extract paste 0.5g, pH7.5,120 ℃ of sterilizing 30min.
In fermenting process of the present invention, the inoculum size of rhodococcus erythropolis is 5%, and culture temperature is 33 ℃, and air flow is 1.5m
3/ h.
The input amount of microorganism composite flocculation agent of the present invention is 100 mL/L.
Embodiment 3
A microorganism composite flocculation agent, comprises following raw material by weight: 7 parts of rhodococcus erythropolis fermented products, 2mol/L CaCl
290 parts of solution, 5 parts of chitosans.
The product wadding substratum (g/L) using in above-mentioned rhodococcus erythropolis fermenting process: glucose 10g, K
2hPO
42g, KH
2pO
42g, MgSO
47H
2o 0.2g, CaCl
20.1g, urea 0.5g, yeast extract paste 0.5g, pH7.2,120 ℃ of sterilizing 30min.
In fermenting process of the present invention, inoculum size is 3%, and culture temperature is 28 ℃, and air flow is 1.5m
3/ h.
The input amount of microorganism composite flocculation agent of the present invention is 125mL/L.
The mensuration of test example 1 flocculating effect and flocculating rate
1, (pH is 7.8, and turbidity is 290NTU, and COD is 415.4mg/L, and colourity is 357 times, and specific conductivity is 8.9 * 10 to get respectively 150mL textile sewage
2μ Scm
-1) be placed in 250mL Erlenmeyer flask, at 25 ℃, drop into wherein a certain amount of flocculation agent, flocculation agent comprises the composite flocculation agent of embodiment 1, embodiment 2, embodiment 3 preparations, and control sample is chitosan, by flocculation agent concussion or after rocking 20min, after sedimentation flocculation 30min, takes sample.
2, turbidity adopts GB13200-91 spectrophotometric determination; Colourity adopts platinum cobalt State Standard Colorimetry to measure, fixed by YS-2000 liquid colourity instrumentation; COD adopts potassium dichromate process to measure;
Its calculation formula is as follows:
Turbidity removal/look/COD leads (%)=(1-
a 1 / A 0 ) * 100%
In formula:
a 0 for the value before water sample processing;
a 1 for the value after water sample processing.
Flocculating effect test result is as shown in table 1:
Table 1
Claims (10)
1. a microorganism composite flocculation agent, is characterized in that, comprises following raw material by weight: rhodococcus erythropolis fermented product 5-10 part, 2mol/L CaCl
2solution 70-120 part, chitosan 3-10 part.
2. microorganism composite flocculation agent according to claim 1, is characterized in that, comprises following raw material by weight: rhodococcus erythropolis fermented product 6-8 part, 2mol/L CaCl
2solution 80-110 part, chitosan 4-7 part.
3. microorganism composite flocculation agent according to claim 1, is characterized in that, comprises following raw material by weight: 7 parts of rhodococcus erythropolis fermented products, 2mol/L CaCl
290 parts of solution, 5 parts of chitosans.
4. according to the microorganism composite flocculation agent described in claim 1-3 any one, it is characterized in that the product wadding substratum (g/L) that described rhodococcus erythropolis is used during the fermentation: glucose 10g, K
2hPO
42g, KH
2pO
42g, MgSO
47H
2o 0.2g, CaCl
20.1g, urea 0.5g, yeast extract paste 0.5g, pH7.2-7.5,120 ℃ of sterilizing 30min.
5. microorganism composite flocculation agent according to claim 4, is characterized in that, the pH that produces wadding substratum is 7.4.
6. according to the microorganism composite flocculation agent described in claim 1-3 any one, it is characterized in that, during the fermentation, inoculum size is 3%-5% to described rhodococcus erythropolis, and culture temperature is 28-35 ℃, and air flow is 1.5m
3/ h.
7. microorganism composite flocculation agent according to claim 6, is characterized in that, during the fermentation, inoculum size is 4% to described rhodococcus erythropolis, and culture temperature is 30 ℃.
8. according to the microorganism composite flocculation agent described in claim 1-3 any one, it is characterized in that, the input amount of described flocculation agent is 90-130mL/L; Preferably, input amount is 100 mL/L.
9. microorganism composite flocculation agent according to claim 4, is characterized in that, the input amount of described flocculation agent is 90-130mL/L; Preferably, input amount is 100 mL/L.
10. microorganism composite flocculation agent according to claim 6, is characterized in that, the input amount of described flocculation agent is 90-130mL/L; Preferably, input amount is 100 mL/L.
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|---|---|---|---|
| CN201410420813.6A CN104193010A (en) | 2014-08-25 | 2014-08-25 | Microbial composite flocculant |
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|---|---|---|---|
| CN201410420813.6A CN104193010A (en) | 2014-08-25 | 2014-08-25 | Microbial composite flocculant |
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Family
ID=52078433
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104556404A (en) * | 2014-12-30 | 2015-04-29 | 盐城工学院 | Chitosan-contained biologically compounded flocculant and application thereof |
| CN105712493A (en) * | 2016-04-11 | 2016-06-29 | 江南大学 | Method for treating heavy metal wastewater through bacillus mucilaginosus polysaccharide and chitosan double-component flocculant |
| CN106563424A (en) * | 2016-11-11 | 2017-04-19 | 广西新六合环保有限责任公司 | Sewage treatment agent |
| CN106564982A (en) * | 2016-11-11 | 2017-04-19 | 广西新六合环保有限责任公司 | Waste water treatment agent |
| CN106564977A (en) * | 2016-11-11 | 2017-04-19 | 广西新六合环保有限责任公司 | Industrial waste water treatment agent and preparation method therefor |
| CN106563423A (en) * | 2016-11-11 | 2017-04-19 | 广西新六合环保有限责任公司 | Sewage treating agent |
| CN106587538A (en) * | 2017-01-10 | 2017-04-26 | 上海应用技术大学 | Method for treating excrement and urine wastes |
| CN106587370A (en) * | 2016-11-11 | 2017-04-26 | 广西新六合环保有限责任公司 | Waste water treatment agent |
| CN106630181A (en) * | 2016-11-11 | 2017-05-10 | 广西新六合环保有限责任公司 | Industrial wastewater treating agent and preparation method thereof |
| CN106745351A (en) * | 2016-11-11 | 2017-05-31 | 广西新六合环保有限责任公司 | A kind of Industrial waste water treatment agent and preparation method thereof |
| CN106745350A (en) * | 2016-11-11 | 2017-05-31 | 广西新六合环保有限责任公司 | A kind of Industrial waste water treatment agent |
| CN107746115A (en) * | 2017-11-03 | 2018-03-02 | 常州市万昌化工有限公司 | A kind of preparation method of high flocculating rate composite flocculation agent |
| CN111252905A (en) * | 2020-02-18 | 2020-06-09 | 桂林电子科技大学 | Nanometer traditional Chinese medicine preparation for treating urban river sewage and preparation method thereof |
| CN111484148A (en) * | 2020-06-11 | 2020-08-04 | 肖玉连 | High-efficiency sewage flocculant and preparation method thereof |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104556404A (en) * | 2014-12-30 | 2015-04-29 | 盐城工学院 | Chitosan-contained biologically compounded flocculant and application thereof |
| CN105712493A (en) * | 2016-04-11 | 2016-06-29 | 江南大学 | Method for treating heavy metal wastewater through bacillus mucilaginosus polysaccharide and chitosan double-component flocculant |
| CN105712493B (en) * | 2016-04-11 | 2019-03-01 | 江南大学 | A kind of method of colloid bacillus cereus polysaccharide and chitosan two-component flocculant process heavy metal wastewater thereby |
| CN106564977A (en) * | 2016-11-11 | 2017-04-19 | 广西新六合环保有限责任公司 | Industrial waste water treatment agent and preparation method therefor |
| CN106564982A (en) * | 2016-11-11 | 2017-04-19 | 广西新六合环保有限责任公司 | Waste water treatment agent |
| CN106563423A (en) * | 2016-11-11 | 2017-04-19 | 广西新六合环保有限责任公司 | Sewage treating agent |
| CN106587370A (en) * | 2016-11-11 | 2017-04-26 | 广西新六合环保有限责任公司 | Waste water treatment agent |
| CN106630181A (en) * | 2016-11-11 | 2017-05-10 | 广西新六合环保有限责任公司 | Industrial wastewater treating agent and preparation method thereof |
| CN106745351A (en) * | 2016-11-11 | 2017-05-31 | 广西新六合环保有限责任公司 | A kind of Industrial waste water treatment agent and preparation method thereof |
| CN106745350A (en) * | 2016-11-11 | 2017-05-31 | 广西新六合环保有限责任公司 | A kind of Industrial waste water treatment agent |
| CN106563424A (en) * | 2016-11-11 | 2017-04-19 | 广西新六合环保有限责任公司 | Sewage treatment agent |
| CN106587538A (en) * | 2017-01-10 | 2017-04-26 | 上海应用技术大学 | Method for treating excrement and urine wastes |
| CN107746115A (en) * | 2017-11-03 | 2018-03-02 | 常州市万昌化工有限公司 | A kind of preparation method of high flocculating rate composite flocculation agent |
| CN111252905A (en) * | 2020-02-18 | 2020-06-09 | 桂林电子科技大学 | Nanometer traditional Chinese medicine preparation for treating urban river sewage and preparation method thereof |
| CN111252905B (en) * | 2020-02-18 | 2023-03-28 | 桂林电子科技大学 | Nanometer traditional Chinese medicine preparation for treating urban river sewage and preparation method thereof |
| CN111484148A (en) * | 2020-06-11 | 2020-08-04 | 肖玉连 | High-efficiency sewage flocculant and preparation method thereof |
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