CN1046946C

CN1046946C - Method fro preparing raw material of nucleic acid from natural food

Info

Publication number: CN1046946C
Application number: CN96115417A
Authority: CN
Inventors: 吴文国
Original assignee: Individual
Current assignee: Individual
Priority date: 1996-06-26
Filing date: 1996-06-26
Publication date: 1999-12-01
Anticipated expiration: 2016-06-26
Also published as: CN1169434A

Abstract

The present invention relates to a method for preparing an organic compound, particularly to a method for preparing nucleic acid from natural food. The method comprises the processing steps of raw material grinding, homogenization, cracking, centrifugation, nucleic acid preparation, etc., and is characterized in that the cracking of cells and neucleoprotein is carried out, and nuclei acid macromolecules are cut in a physical or chemical mode in the alkaline state of using sodium chloride solution as solvent by adopting the physical methods of grinding, homogenization at a high temperature and high speed, etc. The method has the advantages of high extraction rate, simple technological method, low production cost, short production period and abundant material source, and is especially suitable for industrialized large-scale production.

Description

From whole food, produce the method for nucleic acid raw material

The present invention relates to a kind of method for preparing organic compound, particularly utilize physics, chemical process from whole food, to produce nucleic acid raw material.

It is 94115437.8 that Chinese invention patent discloses a kind of application number, name is called the production technique of extracting nucleic acid from natural plant cells, it is raw material with the de-fatted soybean dregs, through swelling, the homogenate preparation, lysis, solvent extraction, dialysis treatment, concentration and purity testing, the sterilization packing, production technique such as drying make nucleic acid extraction liquid and nucleic acid purification dry product, its deficiency is: utilize chemical reagent such as phenol or ethanol extracting, extracting solution need carry out dialysis treatment, usually need 48～72 hours, and every dialyzate of replacing in 6～8 hours, its production cycle is long, the cost height, the discharging of harmful liquid also pollutes the environment, and is not suitable for suitability for industrialized production.

It is 931110416 that Chinese invention patent also discloses a kind of application number, title is the preparation method and the application thereof of human placental nucleic acid, this method also is to utilize chemical reagent such as saturated phenol extracting, the dialysis treatment of extracting solution needs 5～7 days usually, its production cycle is long, the cost height, the discharging of harmful liquid also pollutes the environment.Raw material sources are few, can not form large-scale production, and its finished product price is higher.

The object of the present invention is to provide a kind of method of producing the high purity nucleic acid raw material from whole food, its technology is simple, with short production cycle, and raw material sources are extensive.

Purpose of the present invention can realize by following measure:

A kind of method of from whole food, producing nucleic acid raw material, mainly comprise following processing step: A, raw material and preliminary working: get whole food, comprise red bean, mung bean, broad bean, pea, sorghum rice, corn, millet, barley, wheat and fruit, be ground into above meal of 80 orders or mashed prod.B, even matter, cracking: add sodium-chlor (Nacl) solution that contains 0.1～0.25M in the material after pulverizing and dilute, the ratio of material and sodium chloride solution is 1: 10～20 (weight ratios), in diluent, add highly basic or weak base, adjust pH value and make it PH between 8～12, then under 90 ℃～100 ℃ condition, in refiner, spare matter with 8000～12000 rev/mins speed, cracking 40～60 minutes, with the cracking of quickening cell and nucleoprotein and strengthened cutting to nucleic acid molecule, promote proteinic hydrolysis, also carried out high-temperature sterilization simultaneously.C, produce nucleic acid: the gained lysate is transferred between pH value to 6～7 with strong acid or weak acid, input whizzer and centrifugal 10～20 minutes with 1000～3000 rev/mins speed, and the gained supernatant liquor is edible nucleic acid extraction liquid.Also nucleic acid extraction liquid drying can be made the nucleic acid powder, as food fortifier.In nucleic acid extraction liquid, be equipped with thickening material, sweeting agent etc. and can make the natural acid nutrition oral administration.Be equipped with raw materials such as an amount of sweeting agent and dextrin in nucleic acid extraction liquid, drying, granulating process can be made the nutritive nucleic acid electuary.Above-mentioned centrifugal sediment drying be can be used as nucleic acid organic feed or fertilizer.D, the purifying of nucleic acid extraction liquid: get supernatant liquor with 1: 8～15 ratio saturated ammonium sulphate mixed diluting, with 3000～4000 rev/mins speed centrifugal 10～20 minutes, remove protein, in removing proteinic supernatant liquor, add sodium-chlor, the content that makes sodium-chlor in the supernatant liquor is 3～6M, with 3000～4000 rev/mins speed centrifugal 10～20 minutes, adding distil water dilution in the gained precipitate nucleic acids, both ratios are 1: 1～2, remove sodium-chlor with the microbial film vacuum filtration then, the nucleic acid solution of removing sodium-chlor is carried out drying, and resulting product is pure nucleic acid raw material, and this raw material can be used to prepare nucleate cosmetics or pharmaceutical products.

The present invention compared with prior art has following characteristics:

1, adopt physical methods such as grinding, high temperature, at a high speed even matter carrying out the cracking of cell and nucleoprotein and nucleic acid molecule is carried out physics and chemical cutting under as the alkaline state of solvent with sodium chloride solution, its processing method is simple, production cost is low, the cycle is short, each cycle only is 3～4 hours, is fit to industrialized mass.

2, in whole process of production, need not add deleterious chemical reagent and carry out extracting, dialysis, can keep the nutritive ingredient of whole food own, do not pollute the environment again.

3, make nucleic acid purity height.

4, extensive, the low price of raw material sources is suitable for penetration and promotion.

Embodiment:

Producing of nutritive nucleic acid oral liquid: get 50 kilograms in mung bean, be ground into the above meal of 80 orders, add 500 kilograms of sodium-chlor (Nacl) solution dilutions that contain 0.14M, transfer pH value to make it PH between 9～11 with sodium hydroxide NaOH, the alkalescence diluent is under 100 ℃ of conditions, in refiner, spare matter with 10000 rev/mins of speed, cracking 40 minutes, the gained lysate is transferred PH to 7 with hydrochloric acid (HCl), import whizzer then, with 3000 rev/mins speed centrifugal 10 minutes, get supernatant liquor, add edible Gelatinum oxhide and Sodium Cyclamate with 2/1000 ratio respectively, mix the back packing, sterilization, these finished product are the nutritive nucleic acid oral liquid.It is detected, and the gained result is as follows:

Nucleic acid 2.63g/100ml

Protein content 2.94%

Zinc (2n) 4.08mg/kg

Manganese (mn) 1.30mg/kg

Phosphorus (P) 3.33mg/kg

Iron (Fe) 8.46mg/kg

Magnesium (mg) 24.64mg/kg

Sodium (Na) 1108.15mg/kg

Claims

1, a kind of method of producing nucleic acid raw material from whole food is characterized in that:

A, raw material and preliminary working: get mung bean, be ground into above meal of 80 orders or mashed prod;

B, even matter, cracking: add the sodium chloride solution that contains 0.1～0.25M in the material after pulverizing and dilute, the ratio of material and sodium chloride solution is 1: 10～20 (weight ratios), adding highly basic or weak base are adjusted pH value and are made it PH between 8～12 in diluent, under 90 °～100 ℃ condition, in refiner, spare matter, cracking 40～60 minutes then with 8000～12000 rev/mins speed;

C, produce nucleic acid: the gained lysate is transferred between pH value to 6～7 with strong acid or weak acid, and centrifugal then 10～20 minutes, the gained supernatant liquor was edible nucleic acid extraction liquid;

The purifying of D, nucleic acid extraction liquid: get supernatant liquor with 1: 8～15 ratio saturated ammonium sulphate mixed diluting, centrifugal then 10～20 minutes, in removing proteinic supernatant liquor, add sodium-chlor, the content that makes sodium-chlor in the supernatant liquor is 3～6M, centrifugal 10～20 minutes, and adding distil water dilution in the gained precipitate nucleic acids, both ratios are 1: 1-2, remove sodium-chlor with the microbial film vacuum filtration then, the nucleic acid solution of removing sodium-chlor is carried out drying, resulting product is pure nucleic acid raw material.