CN104694396A - Penicillium chrysogenum producing fungi having plant poison activity, preparation method and applications thereof - Google Patents

Penicillium chrysogenum producing fungi having plant poison activity, preparation method and applications thereof Download PDF

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CN104694396A
CN104694396A CN201310657926.3A CN201310657926A CN104694396A CN 104694396 A CN104694396 A CN 104694396A CN 201310657926 A CN201310657926 A CN 201310657926A CN 104694396 A CN104694396 A CN 104694396A
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fungi
dingshi
belongs
plant
chrysogenum
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刘权
秦波
金辉
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Lanzhou Institute of Chemical Physics LICP of CAS
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/80—Penicillium
    • C12R2001/82—Penicillium chrysogenum
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor

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Abstract

The present invention discloses Penicillium chrysogenum producing fungi having plant poison activity, a preparation method and applications thereof. The fungi is Penicillium Chrysogenum and has characteristics of radial wrinkles, white edge mycelium, velvet-like texture, a large number of conidium structure, blue-green color, yellowish exudate and soluble pigment, and slight tawny colony back surface. In addition, the metabolites of the fungi provide significant plant poison activity and nematode contact toxicity activity for other plants.

Description

The Penicllium chrysogenum with vegetable poison activity belongs to fungi and its preparation method and application
Technical field
The present invention relates to a kind of Penicllium chrysogenum with vegetable poison activity and belong to fungi and its preparation method and application, belong to microbial pesticide technical field.
Background technology
According to Food and Argriculture OrganizationFAO, there are about 50,000 kinds, weeds in the whole world, and wherein farmland weed is 8000 kinds.Weeds survived in the ecotopes such as local crop, cultivation, farming, soil, weather and social condition in long-term adaptation, damage to crops from many aspects.They and farm crop fight for water, fertilizer, light etc., occupy on the ground and the space of underground, affect crop photosynthesis, and interference plant growth, affects seed output and quality.In addition, many weeds are again the germ of damage to crops, the intermediate host of insect, if barnyard grass is the vector of planthopper, rice leafhopper, armyworm etc.Weeds are formidable enemies of agriculture production, and weeds do not remove, and finally cause crop failure, and the loss caused then can not be ignored.
Chemical herbicide has the effect feature such as rapid, easy to use, vital role (Zhang Yuju has been played in weed control, Sun Huatian, Wang Chunsheng. weedicide and mixed and farmland weed chemical prevention [M] Beijing thereof: Chinese agriculture press, 2000:3-6).But the continuous application of chemical herbicide, also causes the poisoning of crop, and the rising of resistance weed population, environmental pollution is on the rise.Utilize microbial metabolites, particularly Toxins Produced By Plant Pathogenic Fungi, exploitation microbial herbicide is generally the effect of many targets, be not easy the generation causing Weed Resistance, have that target is with strong points, development-success ratio is high, be easy to the characteristics such as process for processing, become one of focus of campelyco research, and demonstrate good development prospect (Zhang Hongyu. Toxins Produced By Plant Pathogenic Fungi weeding activity present Research. Practaculture Science, 2009,26-10,160-164).
Pine nematode, also known as pine tree wilt disease, pine wilt disease, pine wilt discase, it is the main exotic invasive harmful organism disease of serious harm China pine forest safety, cause the destructive great Forest Disease And Pest Status disease of pine tree, have the title of pine tree " cancer ", one of the world four fully stocked wood disease, and 36 kinds of pine genus plants and 8 kinds of non-pine genus plants can be endangered.Because the morbidity of this disease is rapid, harm is serious, and mortality ratio is high, and preventing and treating extremely difficult, pays close attention in countries in the world, and it is always as the Harmful Quarantine Objects that China is external and internal, is also one of national Harmful preventing and controlling emphasis.According to State Administration of Forestry's statistics (2002), there are 8,170,000 hm in this disease 2add up to cause more than 3,500 ten thousand strain pine deaths, chief threat production of forestry and species diversity, havoc forest ecology effect, and direct economic loss reaches 2,500,000,000 yuan, indirect economic loss reaches 25,000,000,000 yuan of (Chen Shouchang, pine wood nematode disease pathogen and pathogenesis are in progress, Sichuan Forestry science and technology, 2010,31-1,18-25).
In recent years, the many places of China find the withered pine tree phenomenon of unknown cause, personnel are separated after deliberation, find to there is B. mucronatus [Wei Suzhen, Shi Yanmei, the Chen Fengmao very similar to pine wood nematode form in many withered pine trees, B. mucronatus and pathogenic, Agriculture of Anhui science, 2010,38 (36): 20666-20667].From result of study in recent years, B. mucronatus is often wider than pine wood nematode distribution range, is usually that B. mucronatus first occurs, then just has pine wood nematode to occur in areal.Also the pine tree finding many non-pine nematode epidemic-stricken areas in China exists B. mucronatus, and the Pinus massoniana Lamb occurrence of large-area in some places is dead, but without pine wood nematode in dead pine, the research preventing and treating B. mucronatus also becomes new study hotspot.
Plant endogenesis epiphyte derives from plant materials, and by producing antibiotic agents or the toxin of the multiple pathogenic bacteria of opposing, help the infringement of host plant opposing biological species, comprising pathogenetic bacteria, fungi, virus and nematode etc., is important control of plant disease microorganism resource.Therefore plant endogenesis epiphyte metabolite has environment friendly, not easily develops immunity to drugs to target organisms.
Summary of the invention
The Penicllium chrysogenum with vegetable poison activity is the object of the present invention is to provide to belong to fungi and its preparation method and application.
The present invention isolates bacterial strain in plant materials, and the metabolite of bacterial strain is acted on other plant.
The Penicllium chrysogenum with vegetable poison activity belongs to a fungi, and bacterial strain is that Penicllium chrysogenum belongs to penicillium Chrysogenumfungi; This bacterial strain F18 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 12nd, 2013, preservation centre address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode is 100101, and deposit number is CGMCC No. 7451; Bacterial strain F18 belongs to Penicllium chrysogenum and belongs to fungi penicillium Chrysogenum, the radial wrinkle of tool, edge white mycelium, quality is velvet-like, and conidium structure is a large amount of, and blue-greenish colour, have a little light yellow transudate and soluble pigment, bacterium colony reverse side is light tan.
The Penicllium chrysogenum with vegetable poison activity belongs to a preparation method for fungi, it is characterized in that the method priority alcohol, aseptic water washing red clover ( trifolium pratense) blade, then aseptically leaf tissue is pulverized, add sterilized water, obtain red clover leaf tissue suspension, serial dilutions is carried out to suspension, obtain the diluent of different concns, get the different concns diluent of certain volume respectively, uniform application is segmenting from Ma Dingshi culture medium flat plate, flat board is cultivated under specific culture condition, after growing macroscopic fungal colony, the concentration that picking is applicable to counting is dull and stereotyped, count, and by all bacterium colonies purifying on fresh Ma Dingshi substratum, after obtaining pure fungal bacterial strain, do short-term at cryogenic refrigerator or preserve for a long time.
In preparation method of the present invention, red clover blade is immersed in alcohol completely, fully vibrates, then repeatedly rinse with sterilized water.
In preparation method of the present invention, the gradient dilution method of red clover leaf tissue suspension, in units of 10 times of volumes, carries out serial dilution with sterilized water, and obtaining concentration gradient is 10 -1, 10 -2until 10 -8serial dilutions.
In preparation method of the present invention, getting the method for the serial dilutions of certain volume, is 10 from concentration gradient -4-10 -8serial dilution multiple in, get 0.1 ml respectively and be uniformly coated on Ma Dingshi plate culture medium.
In preparation method of the present invention, Ma Dingshi fungi isolation medium is by K 2hPO 43H 2o 1g, MgSO 47H 2o 0.5g, peptone 5g, glucose l0g, agar 15 ~ 20g, water 1000mL, natural pH.
In preparation method of the present invention, Incubation Condition is on Ma Dingshi plate culture medium, and 28 ± 2 DEG C of lucifuges are cultivated.
In preparation method of the present invention, select the concentration plate method being applicable to counting, be in the culture dish of 90 mm at diameter, the colony number of macroscopic single dispersion is at 100-150 CFU.
Described fungal bacterial strain store method, short-term preservation can adopt the line of Ma Dingshi slant medium to preserve at 4 DEG C, if do long-term preservation, can preserve at-70 DEG C in the mixed solution of Ma Dingshi liquid nutrient medium and glycerine, the volume ratio of Ma Dingshi liquid nutrient medium and glycerine is 1:1.
The metabolite that Penicllium chrysogenum provided by the invention belongs to fungi F18 has obvious vegetable poison activity to other plant.
The metabolite that Penicllium chrysogenum provided by the invention belongs to fungi F18 has obvious nematode contact toxicity to other plant.
Weedicide raw material sources of the present invention, in plant rhizosphere soil, have no adverse effects, and production cost is low for all biologies such as environment and plant, people, animals, and production process is simple, free from environmental pollution.
The present invention, for the No-harmful apple orchard of the biological control of weeds, the Application and Development of microbial herbicide and agricultural-food, is extremely important, is suitable for applying.
Nematode of the present invention tags material raw material sources in plant endogenesis epiphyte, have no adverse effects, and production cost is low for all biologies such as environment and plant, people, animals, and production process is simple, free from environmental pollution.
The present invention, for the control of pine wood nematode and B. mucronatus, for the pine nematode solving puzzlement forest development, is extremely important, is suitable for application.
Embodiment
The experimental technique used in following embodiment, is ordinary method if no special instructions.
Embodiment 1. utilizes serial dilutions method separating plant rhizosphere soil fungi
1, the separation of Penicllium chrysogenum endogenetic fungus
Isolated strains F18 from the red clover seed being purchased from Lanzhou Seed Market.Its method is as follows: 1 g seed immerses in the alcohol of 1 volume, fully vibration 5 min, elimination alcohol, with elimination solution after aseptic water washing, after adding 800 μ L water, seed is ground to powder, then in units of 10 times of volumes, carries out serial dilution, until obtaining Cmin is 10 -8serial dilutions, be 10 from concentration gradient -4-10 -8diluent in, getting 0.1 ml is respectively uniformly coated on Ma Dingshi plate culture medium, 28 ± 2 DEG C of lucifuges are cultivated, after flat board growing macroscopic fungal colony, to new Ma Dingshi substratum, purifying is carried out with a little colony lift of sterile toothpick picking, do not pollute, then proceed to Ma Dingshi slant medium, 4 DEG C of short-term preservations.
The concrete moiety of Ma Dingshi substratum and consistent in summary of the invention.
Bacterial strain provided by the present invention is Penicllium chrysogenum (Bacillus sp.) F18.This bacterial strain F18 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 12nd, 2013, preservation centre address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode is 100101, and deposit number is CGMCC No. 7451.
The cultivation of embodiment 2. bacterium and the preparation of metabolite
1, first class inoculum is cultivated: the bacterial strain F18 be kept on slant medium is transferred activation on Ma Dingshi plate culture medium, and 28-30 DEG C of lucifuge is cultivated, as first class inoculum after growing macroscopic bacterium colony.
2, second class inoculum is cultivated: be inoculated into by the first class inoculum through overactivation on Ma Dingshi liquid nutrient medium, being placed on temperature is 28-30 DEG C, rotating speed be 180 rpm shaking table on lucifuge cultivate, routine observation record, become after muddy (about 7 days) until nutrient solution, stop oscillation cultivation, proceeds to 4 DEG C of refrigerator short-term preservations.
3, the preparation of metabolite: after fungal fermented filtrate sterilizing, filters, then extracts by ethyl acetate, and concentrated acetic acid ethyl acetate extract, obtains Penicllium chrysogenum secondary metabolite.
The vegetable poison of embodiment 3. Penicllium chrysogenum F18 secondary metabolite is active
1, the cultivation of test plant
In the present embodiment, plant Arabidopsis thaliana, weeds annual bluegrass and high cogongrass are experiment material in mode.Arabidopis thaliana cultivates after 7 days on MS substratum, carries out vegetable poison activity test; After annual bluegrass and high cogongrass all cultivate 5-7 days on aseptic filter paper, carry out vegetable poison activity test.
2, the vegetable poison of different concns metabolite is active
Experiment is carried out in 24 orifice plates, and the test plant choosing size similar puts into orifice plate, adds the sterilized water of identical amount.Choose the bacterial strain F18 metabolite according to gained in embodiment 2-3, be configured to the DMSO solution of different concns respectively, same volume joins in orifice plate, and to add the DMSO of same volume for negative control, each process repeats 3 times.Within 5-7 days, observe and record plant-growth situation after process, calculating plant inhibiting rate formula is: inhibiting rate (%)=(control group-treatment group)/control group × 100%, experimental result SPSS v16.0 software carries out statistical study.
3, result
(1) metabolite is active to the vegetable poison of plant
Metabolite shows very strong plant inhibit activities to Arabidopis thaliana, also shows stronger plant inhibit activities to common weed annual bluegrass in agriculture production and high cogongrass.Under 100 μ g/mL concentration, more than 80% (table 1) can be reached to Arabidopis thaliana inhibiting rate.
Table 1. F18 metabolite is to the plant inhibiting rate of Arabidopis thaliana, annual bluegrass and high cogongrass
(2) metamorphosis of plant
The plant of test plant is short and small, and root is shorter, the root browning of Activities of Some Plants.
The nematode contact toxicity of embodiment 4. F18 metabolite
1, the cultivation of nematode
In the present embodiment with pine wood nematode and B. mucronatus for experiment material, nematode conventionally cultivates acquisition.After whole culture dish is covered with in nematode breeding, be inverted by culture dish, to adding 3-5 ml sterilized water in culture dish lid, place 6 more than h, nematode enters in sterilized water.Microscopy under the microscope, according to nematode density, carries out suitable dilution, and make microscope magnification 40 times time, the nematode number in every visual field is about 100-200 bar.
2, different concns metabolite is to the contact toxicity of nematode
Experiment is carried out in 24 orifice plates, and test and carry out in 24 orifice plates, the test plant choosing size similar puts into orifice plate, adds the sterilized water of identical amount.Choose the bacterial strain F18 metabolite according to gained in embodiment 2-3, be configured to the DMSO solution of different concns respectively, same volume joins in orifice plate, add the nematode suspension according to obtaining in embodiment 3-1 respectively, the volume ratio of metabolite and nematode suspension is 1:1, to add the DMSO of same volume for negative control, each process repeats 3 times.The death condition of 24h, 48 h and 72 h observed and recorded nematodes after process, 5 visuals field that each observation is different, the nematode death toll of record, calculation formula is: nematode corrected mortality (%)=(treatment group mortality ratio-control group mortality ratio)/(1-control group mortality ratio) × 100%, experimental result SPSS v16.0 software carries out statistical study.
3, to the contact toxicity result of plant nematode
After process, 24 h observe, and the nematode adding metabolite is slow in action, and activity is suppressed, and have small portion nematode dead under only having 100 and 50 mg/mL concentration for the treatment of; After processing 48 h, metabolite plays a role gradually, reaches more than 70% under 100 mg/mL concentration for the treatment of to pine wood nematode contact toxicity, to the contact toxicity of B. mucronatus also close to 60%.
Corrected mortality to pine, B. mucronatus under table 1. different treatment concentration
。

Claims (10)

1. the Penicllium chrysogenum with vegetable poison activity belongs to a fungi, and bacterial strain is that Penicllium chrysogenum belongs to penicillium Chrysogenumfungi; This bacterial strain F18 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on April 12nd, 2013, preservation centre address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode is 100101, and deposit number is CGMCC No. 7451; Bacterial strain F18 belongs to Penicllium chrysogenum and belongs to fungi penicillium Chrysogenum, the radial wrinkle of tool, edge white mycelium, quality is velvet-like, and conidium structure is a large amount of, and blue-greenish colour, have a little light yellow transudate and soluble pigment, bacterium colony reverse side is light tan.
2. a kind of Penicllium chrysogenum with vegetable poison activity belongs to the preparation method of fungi as claimed in claim 1, it is characterized in that the method priority alcohol, aseptic water washing red clover blade, then aseptically leaf tissue is pulverized, add sterilized water, obtain red clover leaf tissue suspension, serial dilutions is carried out to suspension, obtain the diluent of different concns, get the different concns diluent of certain volume respectively, uniform application is segmenting from Ma Dingshi culture medium flat plate, flat board is cultivated under specific culture condition, after growing macroscopic fungal colony, the concentration that picking is applicable to counting is dull and stereotyped, count, and by all bacterium colonies purifying on fresh Ma Dingshi substratum, after obtaining pure fungal bacterial strain, do short-term at cryogenic refrigerator or preserve for a long time.
3. method as claimed in claim 2, is characterized in that red clover blade to immerse completely in alcohol, fully vibrates, then repeatedly rinse with sterilized water.
4. method as claimed in claim 2, it is characterized in that the gradient dilution method of red clover leaf tissue suspension, in units of 10 times of volumes, carry out serial dilution with sterilized water, obtaining concentration gradient is 10 -1, 10 -2until 10 -8serial dilutions.
5. method as claimed in claim 2, it is characterized in that the method for the serial dilutions of getting certain volume, is 10 from concentration gradient -4-10 -8serial dilution multiple in, get 0.1 ml respectively and be uniformly coated on Ma Dingshi plate culture medium.
6. method as claimed in claim 2, is characterized in that Ma Dingshi fungi isolation medium is by K 2hPO 43H 2o 1g, MgSO 47H 2o 0.5g, peptone 5g, glucose l0g, agar 15 ~ 20g, water 1000mL, natural pH.
7. method as claimed in claim 2, is characterized in that culture condition is on Ma Dingshi plate culture medium, and 28 ± 2 DEG C of lucifuges are cultivated.
8. method as claimed in claim 2, it is characterized in that the concentration plate method selecting to be applicable to counting, be in the culture dish of 90 mm at diameter, the colony number of macroscopic single dispersion is at 100-150 CFU.
9. a kind of Penicllium chrysogenum with vegetable poison activity belongs to fungi as claimed in claim 1, it is characterized in that the metabolite of fungi has obvious vegetable poison to other plant active.
10. a kind of Penicllium chrysogenum with vegetable poison activity belongs to fungi as claimed in claim 1, it is characterized in that the metabolite of fungi has obvious nematode contact toxicity to other plant.
CN201310657926.3A 2013-12-09 2013-12-09 Penicillium chrysogenum producing fungi having plant poison activity, preparation method and applications thereof Pending CN104694396A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105349434A (en) * 2015-12-01 2016-02-24 北京市农林科学院 Penicillium purpurogenum for poisoning plant parasitic nematodes and preparing method and application thereof
CN109486685A (en) * 2018-12-04 2019-03-19 海南师范大学 A kind of mangrove cusp sea lotus endogenetic fungus and its application in preparation anti-insect activity terpene crystalline compounds

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102154114A (en) * 2010-12-17 2011-08-17 沈阳农业大学 Penicillium Chrysogenun inducing meloidogyne incognita chitwood resistance in tomatoes and use thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102154114A (en) * 2010-12-17 2011-08-17 沈阳农业大学 Penicillium Chrysogenun inducing meloidogyne incognita chitwood resistance in tomatoes and use thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GOTLIEB D.等: "Dry mycelium of Penicillium chrysogenum protects cucumber and tomato plants against the root-knot nematode Meloidogyne javanica", 《PHYTOPARASITICA》 *
SIDDIQUI ZA 等: "Biocontrol of Meloidogyne incognita on tomato using antagonistic fungi，plant-growth-promoting rhizobacteria and cattle manure", 《PEST MANAGEMENT SCIENCE》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105349434A (en) * 2015-12-01 2016-02-24 北京市农林科学院 Penicillium purpurogenum for poisoning plant parasitic nematodes and preparing method and application thereof
CN105349434B (en) * 2015-12-01 2019-01-15 北京市农林科学院 The penicillium purpurogenum and its preparation method and application of one plant of intoxicating plant parasitical eelworm
CN109486685A (en) * 2018-12-04 2019-03-19 海南师范大学 A kind of mangrove cusp sea lotus endogenetic fungus and its application in preparation anti-insect activity terpene crystalline compounds
CN109486685B (en) * 2018-12-04 2020-10-30 海南师范大学 Mangrove cuspid and sea lotus endophytic fungus and application thereof in preparation of anti-insect active terpenoid crystal compound

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Application publication date: 20150610