CN104689029A - Traditional Chinese medicine composition for treating exogenous high fever and preparation method thereof - Google Patents
Traditional Chinese medicine composition for treating exogenous high fever and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition for treating exogenous high fever and a preparation method thereof. The traditional Chinese medicine composition for treating exogenous high fever is composed of the following active ingredients in parts by weight: 20-30 parts of radix bupleuri, puerarin, 20-30 parts of scutellaria baicalensis, 12-15 parts of rhizoma anemarrhenae, 12-15 parts of honeysuckle, 12-15 parts of fructus forsythia, 20-30 parts of folium isatidis, 20-30 parts of kalmegh, 4-6 parts of licorice and 9-12 parts of artemisia apiacea. The traditional Chinese medicine composition for treating exogenous high fever is a prescription composed of traditional Chinese medicines having the functions of relieving muscles to expel heat, and clearing away heat and toxic materials, the traditional Chinese medicine composition is made into granules, and is quick to expel heat, convenient to use and carry and small in side effect.
Description
Technical field
The present invention relates to tcm field, particularly relate to a kind of Chinese medicine composition being used for the treatment of exogenous high fever and preparation method thereof.
Background technology
Exogenous high fever is common clinical disease, refers to the evil malicious induced Acute heating of diseases caused by exogenous pathogenic factor and body temperature reaches more than 38.5 DEG C persons.Though have comparatively multi-method to the treatment of this disease at present, but still there are some weak links, be worth research further.Western medicine aspect, primary disease belongs to the category of upper respiratory tract infection more, is that conventional antiviral drugs, antibiotic and antipyretic analgesic etc. are treated caused by virus or bacteriological infection.But in Western medicine, antiviral drugs kind is few at present, curative effect also owes desirable, and antibiotic and antipyretic analgesic all have certain side effect.And with regard to clinical findings, after using said medicine treatment, patient temperature gos up repeatedly, and the course for the treatment of is longer.
Chinese medicine has also made a large amount of research work to primary disease, but also there is following weak link.The first, in prescription, how to be made up of some heat-clearing and toxic substances removing medicines, and the product of less use expelling pathogenic factors from muscles for reducing heat.The second, in dosage form, mostly be decoction, oral liquid etc.Inconvenience is carried in decoction decoction.Oral liquid institute content of dispersion is less, is difficult to reach effective therapeutic dose.Therefore efficient, quick-acting, new drug development and application is easily the basic assurance and the task of top priority that improve calentura clinical efficacy.
Therefore, prior art has yet to be improved and developed.
Summary of the invention
In view of above-mentioned the deficiencies in the prior art, the object of the present invention is to provide a kind of Chinese medicine composition being used for the treatment of exogenous high fever and preparation method thereof, to be intended to solve in prior art Chinese medicine and western medicine to the problem existing for treatment exogenous high fever disease.
Technical scheme of the present invention is as follows:
Be used for the treatment of a Chinese medicine composition for exogenous high fever, wherein, by weight, its active component is mainly composed of the following components:
Radix Bupleuri 20-30, Radix Puerariae 20-30, Radix Scutellariae 12-15, Rhizoma Anemarrhenae 12-15, Flos Lonicerae 12-15, Fructus Forsythiae 12-15, Folium Isatidis 20-30, Herba Andrographis 20-30, Radix Glycyrrhizae 4-6, Herba Artemisiae Annuae 9-12.
The described Chinese medicine composition being used for the treatment of exogenous high fever, wherein, by weight, its active component is mainly composed of the following components:
Radix Bupleuri 30, Radix Puerariae 30, Radix Scutellariae 15, the Rhizoma Anemarrhenae 15, Flos Lonicerae 15, Fructus Forsythiae 15, Folium Isatidis 30, Herba Andrographis 30, Radix Glycyrrhizae 6, Herba Artemisiae Annuae 10.
The described Chinese medicine composition being used for the treatment of exogenous high fever, wherein, described in be used for the treatment of exogenous high fever Chinese medicine composition be granule.
The described Chinese medicine composition being used for the treatment of exogenous high fever, wherein, described in be used for the treatment of containing adjuvant in the Chinese medicine composition of exogenous high fever, described adjuvant is dextrin.
Be used for the treatment of a preparation method for the Chinese medicine composition of exogenous high fever as above, wherein, comprise the following steps:
Put into Radix Bupleuri, Radix Puerariae, Radix Scutellariae, the Rhizoma Anemarrhenae, Flos Lonicerae, Fructus Forsythiae, Folium Isatidis, Herba Andrographis, Radix Glycyrrhizae, Herba Artemisiae Annuae, add decocting in water 1.5 hours, filter, collect filtrate, retain medicinal residues; In medicinal residues, add decocting in water again 1 hour, filter, collect filtrate; Merge two batches of filtrates and be concentrated into relative density 1.25, with appropriate dextrin for adjuvant, spray-drying process.
Beneficial effect: the Chinese medicine composition being used for the treatment of exogenous high fever provided by the present invention adopts by expelling pathogenic factors from muscles for reducing heat, the prescription of heat-clearing and detoxifying herb composition, and makes granule, bring down a fever rapidly, use easy to carry, side effect is little.
Detailed description of the invention
The invention provides a kind of Chinese medicine composition being used for the treatment of exogenous high fever and preparation method thereof, for making object of the present invention, technical scheme and effect clearly, clearly, the present invention is described in more detail below.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Adopt in the present invention by expelling pathogenic factors from muscles for reducing heat, the prescription of heat-clearing and detoxifying herb composition, makes granule, to provide a kind of newtype drug of bringing down a fever rapidly, using easy to carry, that side effect is little treatment exogenous high fever.
Particularly, described in be used for the treatment of the Chinese medicine composition of exogenous high fever, by weight, its active component is composed of the following components:
Radix Bupleuri 20-30, Radix Puerariae 20-30, Radix Scutellariae 12-15, Rhizoma Anemarrhenae 12-15, Flos Lonicerae 12-15, Fructus Forsythiae 12-15, Folium Isatidis 20-30, Herba Andrographis 20-30, Radix Glycyrrhizae 4-6, Herba Artemisiae Annuae 9-12.
Wherein, the optimum weight part proportioning being used for the treatment of the Chinese medicine composition of exogenous high fever described in is:
Radix Bupleuri 30, Radix Puerariae 30, Radix Scutellariae 15, the Rhizoma Anemarrhenae 15, Flos Lonicerae 15, Fructus Forsythiae 15, Folium Isatidis 30, Herba Andrographis 30, Radix Glycyrrhizae 6, Herba Artemisiae Annuae 10.
Wherein, Radix Bupleuri expelling pathogenic factors from muscles dispels the heat, and Radix Puerariae diaphoresis expelling pathogenic factors from muscles, Radix Scutellariae, Rhizoma Anemarrhenae clearing interior heat, this four medicine is monarch.Flos Lonicerae, Fructus Forsythiae dispelling wind to relieve the exterior syndrome, and Folium Isatidis, Herba Andrographis, Herba Artemisiae Annuae heat-clearing and toxic substances removing, four medicines are minister, and Radix Glycyrrhizae coordinating the actions of various ingredients in a prescription is for making.The Chinese medicine composition being used for the treatment of exogenous high fever of the present invention, relieving exterior-interior syndrome during treatment exogenous high fever, defends gas and rules together, and pay attention to heat-clearing and toxic substances removing.The described Chinese medicine composition being used for the treatment of exogenous high fever can be used for treating exogenous high fever, simultaneous occurrence of syndromes of both QI and WEI systems; Disease sees that heating very, and micro evil wind is trembled with fear, general pain, excessive thirst, red tongue, yellow fur, floating and rapid pulse.
Clinical observation shows, the Chinese medicine composition being used for the treatment of exogenous high fever has ideal curative effect to simultaneous occurrence of syndromes of both QI and WEI systems type exogenous high fever, and 1 ~ 2h starts to bring down a fever after the tablet has been ingested, and efficiency is better than Western medicine; And stable curative effect, the slow continuous decrease of heating body temperature, body temperature will go up to make the course for the treatment of elongated repeatedly.
The described Chinese medicine composition being used for the treatment of exogenous high fever preferably makes granule.The process making granule is: above-mentioned 10 raw material of Chinese medicine are added decocting in water 1.5 hours, filters, and collects filtrate, retains medicinal residues; In medicinal residues, add decocting in water again 1 hour, filter, collect filtrate; Merge two batches of filtrates and be concentrated into relative density 1.25, with appropriate dextrin for adjuvant, spray-drying process.
Embodiment 1: the preparation being used for the treatment of the Chinese medicine composition of exogenous high fever
The consumption of each herbal medicine raw material is as follows:
Radix Bupleuri 30g, Radix Puerariae 30 g, Radix Scutellariae 15 g, the Rhizoma Anemarrhenae 15 g, Flos Lonicerae 15 g, Fructus Forsythiae 15 g, Folium Isatidis 30, Herba Andrographis 30 g, Radix Glycyrrhizae 6 g, Herba Artemisiae Annuae 10.
Above-mentioned 10 taste raw material of Chinese medicine water are added decocting in water 1.5 hours, filters, collect filtrate, retain medicinal residues; In medicinal residues, add decocting in water again 1 hour, filter, collect filtrate; Merge two batches of filtrates and be concentrated into relative density 1.25, with appropriate dextrin for adjuvant, spray-drying process.Wherein, the content of described granule is 5.0g crude drug/every gram of granule.
Embodiment 2: the Detoxication experiment being used for the treatment of the Chinese medicine composition of exogenous high fever
1, medicine:
Be used for the treatment of the Chinese medicine composition of exogenous high fever, it is 0.45,0.9,1.8g crude drug/mL that the Chinese medicine composition distilled water diluting being used for the treatment of exogenous high fever of gained in embodiment 1 is mixed with concentration;
Aspirin, Hunan pharmaceutical factory produces, specification 0.3g/ sheet; Being mixed with concentration with distilled water diluting is 0.028g/mL;
Typhoid fever-paratyphoid vaccine, Wuhan Biological Products Inst., Ministry of Public Health produces, and specification 5 mL/ props up.
2, animal:
Japan's white big ear rabbit, body weight 1.8-2.2kg, male female for subsequent use, provided by Hu'nan Inst. of Plarmaceutical Industry.
3, experimental technique and result:
First 2 days of rabbit experiment, surveys normal anus temperature every day 2 times, Thermal preference the 38.5-39.5 DEG C of animal that both every day, fluctuation was no more than 0.2 DEG C for testing.First survey animal basal body temperature before experiment, then according to the weight of animals, ear vein injection typhoid fever-paratyphoid vaccine 1.5 mL/kg, when rabbit body temperature raises more than 1 DEG C, is divided into 5 groups at random by animal, often organizes 10.Blank group (1) is set, takes distilled water; Positive controls (2) is set, Aspirin 0.28g/kg; Three groups of experimental grouies are set, are respectively low dose group (3) 4.5g crude drug/kg, middle dosage group (4) 9.0g crude drug/kg, high dose group (5) 18.0g crude drug/kg that take the Chinese medicine composition being used for the treatment of exogenous high fever.After taking medicine, 1h, 2h, 4h, 6h test anus temperature 1 time respectively, respectively organize after administration with administration before body temperature difference (for heat up, positive number is lower the temperature to negative), result is as shown in table 1.
Table 1 is used for the treatment of the impact of Chinese medicine composition on rabbit body temperature of exogenous high fever
(n=10, meansigma methods ± SD value)
* P<0.05 is compared, * * P<0.01 with blank group.
Table 1 result shows, after family's rabbit ear vein injection typhoid fever-paratyphoid vaccine, body temperature on average raises more than 1 DEG C.And after being used for the treatment of the Chinese medicine composition of exogenous high fever, all can make the slow continuous decrease of fever in rabbit body temperature, its generating process of lowering the temperature, dosage is 4.5g/kg, 2h after administration; Dosage is 9 .0g/kg, 1-4h after administration; Dosage is 18.0 g/kg, 1-6h after administration.Compare with matched group, difference has significant.Experimental result shows, described in be used for the treatment of exogenous high fever Chinese medicine composition there is refrigeration function, and the slow continuous decrease of heating body temperature.
Embodiment 3: the antiinflammatory action experiment being used for the treatment of the Chinese medicine composition of exogenous high fever
1, medicine:
Be used for the treatment of the Chinese medicine composition of exogenous high fever, it is 0.39,0.78,1.56g crude drug/mL that the Chinese medicine composition distilled water diluting being used for the treatment of exogenous high fever of gained in embodiment 1 is mixed with concentration.During rat administration, distilled water is mixed with concentration is 0.365,0.735,1.47g crude drug/mL.
Aspirin, Hunan pharmaceutical factory produces, specification 0.3g/ sheet; Being mixed with concentration with distilled water diluting is 5mg/mL.During rat administration, distilled water is mixed with concentration is 5mg/mL.
2, animal:
KM white mice, male, 50, body weight 25-30g, is provided by Hu'nan Inst. of Plarmaceutical Industry;
SD rat, male, 50, body weight 140-180g, is provided by Hu'nan Inst. of Plarmaceutical Industry.
3, experimental technique and result:
(1) on the impact of mouse peritoneal capillary permeability
Mice, body weight 25-30g, male, be divided into 5 groups at random: (1) blank group, take distilled water; (2) positive controls, Aspirin 0.2g/kg; Three groups of experimental grouies are set, take the Chinese medicine composition being used for the treatment of exogenous high fever, (3) low dose group 15.6g crude drug/kg, dosage group 3.12g crude drug/kg, (5) high dose group 62.4g crude drug/kg in (4).
Successive administration 7 days, every day 1 time, after last administration 30min, tail vein injection 2% azovan blue 10mL/kg, ip 0.6% acetic acid 20mL/kg causes inflammation immediately, puts to death mice after 20min.With 5mL normal saline flushing abdominal cavity, after flushing liquor is centrifugal, in 620nm place colorimetric, measure optical density (OD), represent the degree of abdominal cavity capillary permeability with optical density.Result is as shown in table 2.
Table 2 is used for the treatment of the Chinese medicine composition of exogenous high fever to the impact of mouse peritoneal capillary permeability
(n=10, meansigma methods ± SD value)
* P<0.05 is compared, * * P<0.01 with blank group.
As can be seen from the result of table 2, the Chinese medicine composition being used for the treatment of exogenous high fever can obviously suppress the permeability of blood capillary to increase, and has the effect of antiinflammatory.
(2) on the impact of rat Ovum Gallus domesticus album toes swelling
Rat, body weight 140-180g, male, be divided into 5 groups at random: (1) blank group, take distilled water; (2) positive controls, Aspirin 0.1g/kg; Three groups of experimental grouies are set, take the Chinese medicine composition being used for the treatment of exogenous high fever, (3) low dose group 7.3g crude drug/kg, dosage group 14.7g crude drug/kg, (5) high dose group 29.4g crude drug/kg in (4).
Gastric infusion, once a day, continuous 7 days.30min after last administration, with 10% Ovum Gallus domesticus album, every rat toes subcutaneous injection 0.05mL.With capillary tube amplifying method measure rat cause inflammation before, cause scorching after 1,2,4,6h toes volume.Calculate change in volume value, thus scorching after with cause scorching before toes volume differences be swelling, the difference between more each group, result is as shown in table 3.
Table 3 is used for the treatment of the Chinese medicine composition of exogenous high fever to the impact of rat toes swelling
(n=10, meansigma methods ± SD value)
* P<0.05 is compared, * * P<0.01 with blank group.
From the results shown in Table 3, be used for the treatment of the Chinese medicine composition of exogenous high fever, dosage is 2h after the Yu Zhiyan of 7.3g crude drug/kg, dosage is 14.7,1-6h after the Yu Zhiyan of 29.4g crude drug/kg, obviously can suppress the swelling of rat Ovum Gallus domesticus album toes, have antiinflammatory action.
To sum up, be used for the treatment of the Chinese medicine composition 31.2 of exogenous high fever, 62.4g crude drug/kg, can obviously reduce acetic acid cause scorching after azovan blue content in mouse peritoneal transudate, represent the permeability that can suppress abdominal cavity blood capillary.Be used for the treatment of the Chinese medicine composition 14.7 of exogenous high fever, 29.4g crude drug/kg, obviously can suppress the swelling of rat Ovum Gallus domesticus album toes, there is the effect of antiinflammatory.
Embodiment 4: antibacterial, the Antiviral breeding that are used for the treatment of the Chinese medicine composition of exogenous high fever
One, experiment material
1, medicine:
Be used for the treatment of the Chinese medicine composition of exogenous high fever: the Chinese medicine composition being used for the treatment of exogenous high fever of gained in embodiment 1.During experiment, physiological saline solution is mixed with concentration is 1g crude drug/mL.
Ampicillin: Harbin Pharmaceutical Factory produces, and content 1g dry powder/bottle, is mixed with 5 μ g/ mL with physiological saline solution during experiment.
Gentamycin sulfate: Hunan Dongting Pharmaceutical Co., Ltd., content 40,000 u/ mL, is mixed with 10 μ g/mL with physiological saline solution during experiment.
Virazole: Tianmen, Hubei pharmaceutical factory, content 100mg/mL, Hank ' s liquid dilution during experiment, then be diluted to 100 μ g/mL with containing 2% little girl's serum 1640 Growth of Cells maintenance medium.
2, animal:
NIH mice, 18-22g, is provided by Hunan College of Traditional Chinese Medicine's Experimental Animal Center.
3, strain:
Staphylococcus aureus, escherichia coli, Diplococcus pneumoniae, alpha streptococcus, beta hemolytic streptococcus, hemophilus influenza is all microorganism Clinical Lab isolation identification strain, and in each corresponding fluid medium after Zengjing Granule, counting bacterial population, goes 1 × 10
9/ mL bacterium number is for experiment.
4, virus:
FM
1(influenza virus A-prime Mus lung adapted strain), is provided by Hunan Medical University's virus research room, calculates virus titer for experiment at Hela passage.
Two, experimental technique and result
1, antibacterial experiment: measure minimum inhibitory concentration (MIC) with test tube method, measures antibacterial to the sensitivity of susceptibility with agar diffusion method (punch method).Staphylococcus aureus in experiment, escherichia coli ordinary broth (agar) culture medium; Diplococcus pneumoniae, alpha streptococcus, beta hemolytic streptococcus serum broth (blood agar plate) culture medium; Hemophilus influenza is with using serum broth (CB agar is dull and stereotyped) culture medium.
Test tube method: after making doubling dilution in the culture medium adapted each bacterium by experimental drug, often pipe adds exponential phase is severe 0.1mL(bacteria containing amount 1 × 10
9/ mL).Observe the minimum inhibitory concentration (MIC) of medicine after 37 DEG C of cultivation 24h, establish culture medium to contrast during experiment, add bacterium not dosing positive control.
Agar diffusion method (punch method): cut-off footpath is 75mm plate, the corresponding culture medium 20mL needed for each bacterial growth poured into by every ware, and after culture medium condensation, dipping bacteria containing amount with aseptic cotton carrier is 1 × 10
9/ mL bacterium liquid, spread plate, punches on plating medium with diameter 6mm card punch, and every hole micro sample adding appliance adds 150 μ L medicinal liquids, puts 37 DEG C and cultivates 24h survey antibacterial circle diameter size, in triplicate, calculate average diameter of inhibition zone.
Be used for the treatment of in the Chinese medicine composition Antimicrobial test of exogenous high fever, the result of minimum inhibitory concentration is as shown in table 4.As can be seen from Table 4, the Chinese medicine composition being used for the treatment of exogenous high fever is the strongest to Diplococcus pneumoniae inhibitory action, and MIC is 62.50mg/mL, to MIC average out to 104.58 mg/mL of 5 strain staphylococcus aureuses, to first, second streptococcus, the MIC of hemophilus influenza is 125 mg/mL.To escherichia coli then unrestraint effect.
Table 4 is used for the treatment of the comparison of the Chinese medicine composition minimum inhibitory concentration of exogenous high fever
| Bacterium name (strain number) | MIC（mg/mL） |
| Staphylococcus aureus (5) | 104.58 |
| Escherichia coli | - |
| Diplococcus pneumoniae | 62.50 |
| Alpha streptococcus | 125.00 |
| Beta hemolytic streptococcus | 125.00 |
| Hemophilus influenza | 125.00 |
Be used for the treatment of in the Chinese medicine composition Antimicrobial test of exogenous high fever, be used for the treatment of the bacteriostasis of Chinese medicine composition in agar plate of exogenous high fever, test result is as shown in table 5.Show from the data of table 5, the bacteriostasis being used for the treatment of the Chinese medicine composition of exogenous high fever conforms in test tube method experimental result, all inhibitory action is had to staphylococcus aureus, first, second streptococcus, Diplococcus pneumoniae and hemophilus influenza, and to escherichia coli unrestraint effect.Compare with the antibacterial matched group ampicillin of the positive, have difference (p is less than 0.01), ampicillin antibacterial action is better than the Chinese medicine composition being used for the treatment of exogenous high fever, and negative control hole (normal saline) is without bacteriostasis.
Table 5 is used for the treatment of the comparison (n=10) of antibacterial circle diameter size in the Chinese medicine composition agar plate of exogenous high fever
Compare with ampicillin, * P < 0.01.
2, Antiviral breeding
2.1 antiviral study in vitros: by " cytopathic effect " (CPE) method, Hale cell first measure medicine to the maximal non-toxic concentration of cell for experiment, then measure the TCID of virus
50(half cytopathic effect unit), then measures the inhibition index of drug on viral, covers with the Hale cell of monolayer, after washing three times with Hank ' s liquid, inoculate 10 times of serial virus liquids, 37 DEG C of absorption are after 1 hour, discard the virus liquid do not adsorbed, if normal cell controls, virus control group practices group, adds Growth of Cells maintenance medium respectively, pastille Growth of Cells maintenance medium, 37 DEG C of cultivations, observation of cell pathological changes 7-8 days, calculates TCID when cytopathy is no longer in progress respectively day by day
50, calculate inhibition index, inhibition index=matched group Log TCID
50-experimental group Log TCID
50, inhibition index>=2, indicate antivirus action.
Antiviral breeding in 2.2 Mice Bodies: NIH mice, body weight 18-22g, is divided into experimental group (point high, medium and low dosage), virazole matched group, Normal group 1(normal saline at random), Normal group 2(1640), virus control group (V+1640).With 10
4.32tCID
50fM
1drop nose infecting mouse, continuous 6 days per os gastric infusions, feedwater, the 7th day mouse weights, solution takes lung, calculates Lung Exponent value (heavy ÷ body weight × 100 of lung), and obtains lung index.Lung Exponent value is large, represents that lung lesion degree is serious.
Lung index (%)=(virus control group Lung Exponent value-experimental group Lung Exponent value) ÷ virus control group Lung Exponent value × 100%
2.3 experimental result
2.3.1 anti-FM in cultured cell
1effect
Be used for the treatment of the minimum non-toxic concn of Chinese medicine composition to cell of exogenous high fever: will the Chinese medicine composition of exogenous high fever be used for the treatment of according to 1:10, 1:20, 1:40, 1:80, 1:160 concentration joins cultivation 24h and has covered with in monolayer Hale cell, every bottle of 1mL, establish simultaneously and do not add medicinal liquid normal cell and compare, 37 DEG C of cultivations, within continuous 7 days, observe (every 48h changes the medicine maintenance medium 1 time containing same dose), make cell suspension, 0.5% tire expects that blue dyeing calculates inhibitory rate of cell growth, 10% liquor strength is less than as experimental concentration using inhibitory rate of cell growth, this minimum non-toxic concn is 12.5mg/mL.
2.3.2 the Chinese medicine composition of exogenous high fever is used for the treatment of to FM
1the measurement result of inhibition index is as shown in table 6.Table 6 result shows, is used for the treatment of the Chinese medicine composition of exogenous high fever to FM
1, in Hale cell, have stronger inhibitory action, its inhibition index difference in means > 2, compares with virus control group, through t inspection, and meaning that there was a significant difference (P < 0.05).
Table 6 is used for the treatment of the Chinese medicine composition of exogenous high fever to FM
1inhibitory action compare (n=4, meansigma methods ± S)
Compare with virus control group, * P < 0.05, * * P < 0.01.
2.3.3 the Chinese medicine composition of exogenous high fever is used for the treatment of on the impact of mouse influenza virus pneumonia
The high, medium and low dosage of Chinese medicine composition being used for the treatment of exogenous high fever all can alleviate the pneumonia of influenza infection mice, as shown in table 7.
Table 7 is used for the treatment of the Chinese medicine composition of exogenous high fever to mouse influenza virus pneumonia impact (n=4, meansigma methods ± S)
Compare with virus control group, * P < 0.05, * * P < 0.01.
2.3.4 the protective effect of the Chinese medicine composition influenza virus infected of exogenous high fever is used for the treatment of
Be used for the treatment of the Chinese medicine composition object height of exogenous high fever, in, low dosage, the existence of influenza virus infected all has certain protective effect, after laboratory observation influenza infection group 10 Murine Virus infect one week interior dead 7, and feed the Chinese medicine composition object height being used for the treatment of exogenous high fever, after the experimental group of middle dosage and the Murine Virus of Ribavirin group infect, none is only dead, after the Chinese medicine composition low dose group Murine Virus being used for the treatment of exogenous high fever infects, 10 have merely hit 2 death, result is as shown in table 8, experimental group, Ribavirin group mouse survival number compares with infection group and viral infection group mouse survival number, meaning (corrected X that there was a significant difference
2inspection P < 0.01).
Table 8 is used for the treatment of the impact of the Chinese medicine composition influenza virus infected survival rate of exogenous high fever
| Number of animals (only) | Dead mouse number | Mouse survival number | |
| Virus control group | 10 | 7 | 3 |
| Ribavirin group | 10 | 0 | 10* |
| Be used for the treatment of the Chinese medicine composition (high dose) of exogenous high fever | 10 | 0 | 10* |
| Be used for the treatment of the Chinese medicine composition (middle dosage) of exogenous high fever | 10 | 0 | 10* |
| Be used for the treatment of the Chinese medicine composition (low dosage) of exogenous high fever | 10 | 2 | 8* |
| Saline control group | 10 | 0 | 10 |
| 1640 matched groups | 10 | 0 | 10 |
Compare with virus control group, * P < 0.01.
To sum up, the Chinese medicine composition vitro antibacterial activity of exogenous high fever is used for the treatment of: to escherichia coli without bacteriostasis; To staphylococcus aureus, Diplococcus pneumoniae, alpha streptococcus, beta hemolytic streptococcus, hemophilus influenza all has stronger bacteriostasis, and its MIC is respectively 104.58 mg/mL, 62.50mg/mL, 125 mg/mL, 125 mg/mL, 125 mg/mL.The minimum non-toxic concn of Chinese medicine composition to experimental cell being used for the treatment of exogenous high fever is 12.5mg/mL.The Chinese medicine composition being used for the treatment of exogenous high fever in cultured cell to FM
1inhibition index be 2.79, comparing difference with virus control group has significant (P < 0.05).Be used for the treatment of the Chinese medicine composition of exogenous high fever to FM
1the existence of infecting mouse has protective effect, and the high, medium and low dosage of experimental group, compares with infection group and viral infection group, mouse survival number there was a significant difference meaning (P < 0.05).The Chinese medicine composition being used for the treatment of exogenous high fever all can alleviate influenza infection mouse lung inflammation, and its lung index compares with virus control group, meaning that there was a significant difference (P < 0.05).
Should be understood that, application of the present invention is not limited to above-mentioned citing, for those of ordinary skills, can be improved according to the above description or convert, and all these improve and convert the protection domain that all should belong to claims of the present invention.
Claims (5)
1. be used for the treatment of a Chinese medicine composition for exogenous high fever, it is characterized in that, by weight, its active component is mainly composed of the following components:
Radix Bupleuri 20-30, Radix Puerariae 20-30, Radix Scutellariae 12-15, Rhizoma Anemarrhenae 12-15, Flos Lonicerae 12-15, Fructus Forsythiae 12-15, Folium Isatidis 20-30, Herba Andrographis 20-30, Radix Glycyrrhizae 4-6, Herba Artemisiae Annuae 9-12.
2. the Chinese medicine composition being used for the treatment of exogenous high fever according to claim 1, is characterized in that, by weight, its active component is mainly composed of the following components:
Radix Bupleuri 30, Radix Puerariae 30, Radix Scutellariae 15, the Rhizoma Anemarrhenae 15, Flos Lonicerae 15, Fructus Forsythiae 15, Folium Isatidis 30, Herba Andrographis 30, Radix Glycyrrhizae 6, Herba Artemisiae Annuae 10.
3. the Chinese medicine composition being used for the treatment of exogenous high fever according to claim 1 and 2, is characterized in that, described in be used for the treatment of exogenous high fever Chinese medicine composition be granule.
4. the Chinese medicine composition being used for the treatment of exogenous high fever according to claim 1 and 2, is characterized in that, described in be used for the treatment of containing adjuvant in the Chinese medicine composition of exogenous high fever, described adjuvant is dextrin.
5. be used for the treatment of a preparation method for the Chinese medicine composition of exogenous high fever as claimed in claim 1, it is characterized in that, comprise the following steps:
Put into Radix Bupleuri, Radix Puerariae, Radix Scutellariae, the Rhizoma Anemarrhenae, Flos Lonicerae, Fructus Forsythiae, Folium Isatidis, Herba Andrographis, Radix Glycyrrhizae, Herba Artemisiae Annuae, add decocting in water 1.5 hours, filter, collect filtrate, retain medicinal residues; In medicinal residues, add decocting in water again 1 hour, filter, collect filtrate; Merge two batches of filtrates and be concentrated into relative density 1.25, with appropriate dextrin for adjuvant, spray-drying process.
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| CN106237155A (en) * | 2016-09-07 | 2016-12-21 | 四川金堂海纳生物医药技术研究所 | A kind of decoction medicine treating exogenous high fever and preparation method thereof |
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| CN106237155A (en) * | 2016-09-07 | 2016-12-21 | 四川金堂海纳生物医药技术研究所 | A kind of decoction medicine treating exogenous high fever and preparation method thereof |
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