CN104688793A - Preparation method of artemisia argyi flavonoid - Google Patents
Preparation method of artemisia argyi flavonoid Download PDFInfo
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- CN104688793A CN104688793A CN201310660962.5A CN201310660962A CN104688793A CN 104688793 A CN104688793 A CN 104688793A CN 201310660962 A CN201310660962 A CN 201310660962A CN 104688793 A CN104688793 A CN 104688793A
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Abstract
The invention relates to a preparation method of artemisia argyi flavonoid, and belongs to the field of plant extraction. The preparation method comprises the following steps: (1) airing artemisia argyi, crushing, extracting with ethanol water, concentrating an extract liquid, extracting, evaporating and drying to obtain a crude product; and (2) separating the crude product with a positive-phase silica gel column to obtain a pure product, wherein the mobile phase is n-hexane and ethyl acetate; the volume ratio of n-hexane to ethyl acetate is 100 to (10-20); the sampling amount is 0.1-1g of crude product in 100g of silica gel; and the elution volume is 8-10-column volume. The preparation method has the beneficial effects that the prepared artemisia argyi flavonoid is few in impurity variety, high in purity and high in yield.
Description
Technical field
The present invention relates to a kind of preparation method of flavonoids of artemisia argyi, belong to field of plant extraction.
Background technology
Effect of flavone is many-sided, it is a kind of very strong antioxidant, can oxygen-derived free radicals effectively in purged body, as anthocyanidin can overflow in full stage of oil-control peroxide, the ability of this prevention oxidation is more than ten times of vitamin E, this antioxidation can stop degeneration, the aging of cell, also can stop the generation of cancer.
Summary of the invention
The method that the present invention is separated by normal phase silicagel column, prepares the flavonoids of artemisia argyi that purity is high, yield is high, dopant species is few.
The invention provides a kind of preparation method of flavonoids of artemisia argyi, described preparation method comprises the steps:
1. Radix Artemisia ordosicae dried, pulverize, ethanol water extracts, extracting solution is concentrated, extraction, evaporation drying obtain crude product;
2. be separated by crude product normal phase silicagel column and obtain sterling, described mobile phase is normal hexane and ethyl acetate, and the volume ratio of normal hexane and ethyl acetate is 100:10 ~ 20, and applied sample amount is 0.1 ~ 1g crude product/100g silica gel, and elution volume is 8 ~ 10 column volumes.
The flavonoids of artemisia argyi purity that the present invention adopts normal-phase chromatography method to obtain is high, yield is high, dopant species is few, and compared with the prior art, existing separation method does not reach technique effect of the present invention.
Ethanol water alcoholic strength of the present invention is preferably 85 ~ 95.
Described extracting solution of the present invention is preferably concentrated into 1/4 ~ 1/2 of original volume.
Described extractant of the present invention is preferably dichloromethane or ethyl acetate.
Beneficial effect of the present invention is:
1. the flavonoids of artemisia argyi purity that obtains of preparation method of the present invention is high, yield is high, dopant species is few;
2. present invention process is simple, strong operability, is applicable to industrialized great production.
Detailed description of the invention
Following non-limiting example can make the present invention of those of ordinary skill in the art's comprehend, but does not limit the present invention in any way.
Embodiment 1
A preparation method for flavonoids of artemisia argyi, described preparation method comprises the steps:
1. 100 ~ 200 orders are crushed to after being dried by Radix Artemisia ordosicae, three times are extracted with the ethanol water of alcoholic strength 90, merge three extracting solution and be concentrated into 1/3 of original volume, extract concentrated solution three times with dichloromethane equal-volume, the drying of distilling under reduced pressure evaporate to dryness extraction phase final vacuum obtains crude product;
2. crude product is separated by the applied sample amount normal phase silicagel column of 0.1g crude product/100g silica gel and obtains sterling, described mobile phase is normal hexane and ethyl acetate, the volume ratio of normal hexane and ethyl acetate is 100:10, elution volume is 8 column volumes, collect once by every 1/4 column volume of the order be separated, collect 32 fractions altogether, after TLC detects, the fraction containing flavonoids of artemisia argyi is merged distilling under reduced pressure final vacuum dry.
It is 98.8% that liquid chromatography detects flavonoids of artemisia argyi content, and yield is 95.7%.
Embodiment 2
A preparation method for flavonoids of artemisia argyi, described preparation method comprises the steps:
1. be crushed to 100 ~ 200 orders after being dried by Radix Artemisia ordosicae, extract three times with the ethanol water of alcoholic strength 90, merge three extracting solution and be concentrated into 1/3 of original volume, extract concentrated solution three times with dichloromethane equal-volume, the drying of distilling under reduced pressure final vacuum obtains crude product;
2. crude product is separated by the applied sample amount normal phase silicagel column of 1g crude product/100g silica gel and obtains sterling, described mobile phase is normal hexane and ethyl acetate, the volume ratio of normal hexane and ethyl acetate is 100:10, elution volume is 10 column volumes, collect once by every 1/4 column volume of the order be separated, collect 40 fractions altogether, after TLC detects, the fraction containing flavonoids of artemisia argyi is merged distilling under reduced pressure final vacuum dry.
It is 92.9% that liquid chromatography detects flavonoids of artemisia argyi content, and yield is 90.4%.
Claims (4)
1. a preparation method for flavonoids of artemisia argyi, described preparation method comprises the steps:
1. Radix Artemisia ordosicae dried, pulverize, ethanol water extracts, extracting solution is concentrated, extraction, evaporation drying obtain crude product;
2. be separated by crude product normal phase silicagel column and obtain sterling, described mobile phase is normal hexane and ethyl acetate, and the volume ratio of normal hexane and ethyl acetate is 100:10 ~ 20, and applied sample amount is 0.1 ~ 1g crude product/100g silica gel, and elution volume is 8 ~ 10 column volumes.
2. preparation method according to claim 1, is characterized in that: described ethanol water alcoholic strength is 85 ~ 95.
3. preparation method according to claim 1, is characterized in that: described extracting solution is concentrated into 1/4 ~ 1/2 of original volume.
4. preparation method according to claim 1, is characterized in that: described extractant is dichloromethane or ethyl acetate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310660962.5A CN104688793A (en) | 2013-12-05 | 2013-12-05 | Preparation method of artemisia argyi flavonoid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310660962.5A CN104688793A (en) | 2013-12-05 | 2013-12-05 | Preparation method of artemisia argyi flavonoid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104688793A true CN104688793A (en) | 2015-06-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310660962.5A Pending CN104688793A (en) | 2013-12-05 | 2013-12-05 | Preparation method of artemisia argyi flavonoid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107854507A (en) * | 2017-11-06 | 2018-03-30 | 山东省中医药研究院 | A kind of method that flavones ingredient is extracted from folium artemisiae argyi |
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2013
- 2013-12-05 CN CN201310660962.5A patent/CN104688793A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107854507A (en) * | 2017-11-06 | 2018-03-30 | 山东省中医药研究院 | A kind of method that flavones ingredient is extracted from folium artemisiae argyi |
| CN107854507B (en) * | 2017-11-06 | 2021-01-22 | 山东省中医药研究院 | Method for extracting flavonoid component from folium artemisiae argyi |
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| Date | Code | Title | Description |
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| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150610 |