CN104686215B - Method for increasing content of carotenoids in tomato fruits - Google Patents
Method for increasing content of carotenoids in tomato fruits Download PDFInfo
- Publication number
- CN104686215B CN104686215B CN201510070026.8A CN201510070026A CN104686215B CN 104686215 B CN104686215 B CN 104686215B CN 201510070026 A CN201510070026 A CN 201510070026A CN 104686215 B CN104686215 B CN 104686215B
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- China
- Prior art keywords
- melatonin
- carotene
- content
- solution
- fructus lycopersici
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the cultivation of crops, and particularly provides a method for increasing the content of carotenoids in tomato fruits. According to the method, a melatonin solution with a concentration of 50-500 microns per liter is used for spraying tomatoes in a mature green stage. The experimental study results show that the spraying of the melatonin solution for the tomatoes in the mature green stage can increase the contents of alpha-carotene and beta-carotene in the tomato fruits significantly. The method is a non-transgenic technology, and is high in safety, high in efficiency, easy in operation and easy in promotion; the used melatonin solution has the characteristics of convenient preparation and biodegradability.
Description
Technical field
The present invention relates to breed of crop, specifically, is related to a kind of side of carotenoid content in raising tamato fruit
Method.
Background technology
With the continuous improvement of people's living standard, for the green of food and the pursuit level of health are also carried constantly
Rise.In addition the continuous reinforcement of people's brought harm understanding unbalance for interior free yl, the removing free radical of fruit and vegerable, antioxygen
Change ability is gradually highlighted.Antioxidant content, the activity research of fruit and vegerable day by day becomes people's concern, the focus of research.
Because carotenoid is primarily present in fresh vegetabless and fruit, and animal and human body itself can not synthesize, and
Newest test it is also shown that human body supplement the α of extraneous chemosynthesis-, beta-carotene, there is no effect to human body function of anti-cancer and cancer prevention.Cause
This, from nature find rich in natural α-, the plant resourceses of beta-carotene, be increasingly subject to the attention of people.Alpha-carotene,
Beta-carotene is powerful antioxidant, the disease that human body can be suppressed relevant with aging, reduces the sickness rate of some cancers.Human body
Contain alpha-carotene, beta-carotene in blood, but human body can not voluntarily synthesize, and absorb mainly by food.Therefore, raising kind
α in solanberry reality-, content beta-carotene there is important using value.
Patent publication No. discloses a kind of raising carotenoid content of tomato for the Chinese patent application of CN101979601A
Method, build the cryptochrome gene 1, c-terminuses of cryptochrome gene 1 and cryptochrome gene 2 using genetic engineering means
C-terminuses Overexpression vector, convert Fructus Lycopersici esculenti, obtain transgenic Fructus Lycopersici esculenti.Realize lycopene in Transgenic tomato fruit
And the raising by a relatively large margin of the content of total carotenoid.
But transgenic technology is not appropriate for enterprising in tomato cultivation due to its operation and the complexity of product safety identification
Row is promoted.
The content of the invention
In order to solve problems of the prior art, it is an object of the invention to provide a kind of improve class Hu in tamato fruit
The method of Radix Raphani cellulose content.
In order to realize the object of the invention, present invention firstly provides a kind of side for improving carotenoid content in tamato fruit
Method, methods described is green ripe stage Fructus Lycopersici esculenti to be carried out using melatonin solution spray process.
Further, the concentration of the melatonin solution is 50 μM～500 μM.
Preferably, the concentration of the melatonin solution is 100 μM.
Further, the amount of spraying of the melatonin solution is 100ml/10 Fructus Lycopersici esculenti.
Further, melatonin solution is carried out to green ripe stage Fructus Lycopersici esculenti per 3 days and sprays process once.
Further, the carotenoid is alpha-carotene and beta-carotene.
Present invention also offers application of the melatonin in tamato fruit is improved in carotenoid content.
Specifically, carrying out spraying process to green ripe stage Fructus Lycopersici esculenti using the melatonin solution that concentration is 50 μM～500 μM.
Preferably, the concentration of the melatonin solution is 100 μM.
Further, the carotenoid is alpha-carotene and beta-carotene.
The beneficial effects of the present invention is：
By the present invention in that spraying process green ripe stage tamato fruit with melatonin, detect after room storage in the red ripe phase
Tamato fruit is processed, discovery can significantly improve alpha-carotene and content beta-carotene in tamato fruit and (divide compared with the control
Do not improve 2.45 times and 3.40 times).Methods described is non-transgenic technology, safe, efficiency high, easy to operate is easily pushed away
Extensively, that the melatonin solution that used is prepared is convenient, with biodegradability the characteristics of.
The present invention is while Quality of Tomato Fruit is improved, it is ensured that the edible safety of Fructus Lycopersici esculenti and the people are physically and mentally healthy,
Promote the quality of production that product improves in garden stuff processing enterprise.
Description of the drawings
Fig. 1 is the cross section that variable concentrations melatonin solution sprays tamato fruit after process in the embodiment of the present invention 1.
Fig. 2 be in the embodiment of the present invention 1 without melatonin process and Jing after 100 μM of melatonin are processed α-Hu in tamato fruit
Radix Raphani cellulose content compares.
Fig. 3 be in the embodiment of the present invention 1 without melatonin process and Jing after 100 μM of melatonin are processed β-Hu in tamato fruit
Radix Raphani cellulose content compares.
Specific embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Embodiment 1
1st, variable concentrations melatonin green ripe stage Fructus Lycopersici esculenti is carried out spraying process (be broadly divided into 0 μM, 1 μM, 50 μM, 100 μM and
Respectively progressive is sprayed for 500 μM of 5 concentration, is sprayed once per three days).Green ripe stage tamato fruit to just having adopted back from ground enters
Row cleaning, selects size, the fruit of solid colour is divided into 5 groups, and per group of 10 fruits are black with taking off for isopyknic variable concentrations respectively
Plain solution is sprayed, and room temperature is dark to place.(3 repetitions being provided with per group, matched group is clear water treatment group).Cut after 10 days,
As shown in figure 1, as shown in Figure 1, the Tomato Fruit Color that 0 μM of melatonin solution of Jing is processed is green lake；1 μM of melatonin was processed
Tomato Fruit Color with compare similar, be also green lake；The Tomato Fruit Color that 50 μM of melatonin were processed is orange red；
100 μM similar with the Tomato Fruit Color after 500 μM of concentration melatonin process, is all pink.Considering cost, 100 μM
It is processed as optimum spraying concentration for the treatment of.
2nd, process Fructus Lycopersici esculenti using high-efficient liquid phase chromatogram HPLC measure and compare the endogenous melatonin content of Fructus Lycopersici esculenti.Process
Good tamato fruit is cut open, removes seed, and sarcocarp chopping takes 4g sarcocarp liquid nitrogen grinding, and with methanol extraction, high performance liquid chromatography is surveyed
Endogenous melatonin content, endogenous melatonin concentration is 21.48ng/g FW in the tamato fruit of 100 μM of melatonin process.Research table
Bright, the content of melatonin is different in different tomato varieties, and scope is by 0.6-114.5ng/g FW, so taking off through external source
Endogenous melatonin content is within normal range in the tamato fruit of melanocyte process.
Wherein, melatonin content method for measuring is as follows：
1. methanol method extracts melatonin
4g materials are put in mortar and are fully ground, be added to afterwards in 10mL methanol and fully vibrate, cleaned with ultrasound wave
45 DEG C of instrument, 80Hz ultrasound 35min, then 12000r/min centrifugations 15min, takes supernatant and is dried up with nitrogen, by the remnants for drying up
Material is dissolved in the methanol aqueous solutions of 2mL 5%, treats next step with C18 solid-phase extraction columns to separate melatonin.First with the nothing of 10mL
Water methanol, and 10mL pure water is activating C18 posts.Then, will add in C18 posts 2mL melatonin extracting solution before, and treat to flow completely
After going out, then C18 posts interference impurity is rinsed with 5% methanol aqueous solution of 2mL, finally, will be adsorbed with the methanol aqueous solutions of 2mL 80%
Melatonin in C18 posts is eluted with slower flow velocity, and 0.1 μm of filter screen filtration of eluent Jing is in case upper machine testing.
2. HPLC-MS methods determine melatonin content
Melatonin standard substance (are purchased from into SIGMA companies, purity is the melatonin for 99.5%) being configured to 4ppb (4ng/mL)
Standard solution.Chromatographic column：Alltima C18 (4.6mm × 250mm) are stainless steel column；Mobile phase：50mmol/L Na2HPO4/
H3PO4Buffer (pH 4.5)：Methanol=60:40；Flow velocity：1mL/min；Sample size：10μL；Excitation wavelength：280nm；Transmitting
Optical wavelength：348nm.
3rd, the storage to processing is measured to Antioxidant Enzyme Systems in the tamato fruit of red ripe phase.When processing
Fructus Lycopersici esculenti when be placed into the red ripe phase at room temperature, determine its α-, content beta-carotene, as a result such as Fig. 2, Fig. 3.Can by figure
Know, the tamato fruit that 100 μM of melatonin of Jing are processed compares alpha-carotene and β-Hu in the tamato fruit without melatonin process
Radix Raphani cellulose content, significantly improves 3 times or so.
Wherein, alpha-carotene and content beta-carotene method for measuring are as follows：
First 3 tamato fruit tissues are mixed, vacuum freeze-drying is crushed, accurately weigh tamato fruit lyophilized powder 1g in tool plug three
In the bottle of angle, extracting solution (acetone is added:Ethanol=3:2) 45mL, shakes up, and covers bottle stopper ultrasonic extraction 20min, filter in
In 50mL volumetric flasks and constant volume.Take 20mL filtrates to put in separatory funnel, add 10mL petroleum ether to mix, add 10mL distilled water to enter
Row distribution, takes petroleum ether and mutually puts in rotary evaporation bottle, and remaining water is added to 10mL petroleum ether and is reallocated, and merges 2 oil
Ether is mutually put on Rotary Evaporators, and 40～45 DEG C are evaporated, and is dissolved with 2.0mL isopropanols, and upper machine is determined after filtration.HPLC is Shimadzu
10Avp high performance liquid chromatographs.Chromatographic column：Alltima C18(4.6mm×250mm)；Mobile phase is acetonitrile:Chloroform
(92:8)；Detection wavelength is 450nm；Flow velocity：1.0mL/min；Column temperature：35℃.Standard substance alpha-carotene, beta-carotene are purchased
In sigma companies.
Although above with a general description of the specific embodiments the present invention is described in detail,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, without departing from theon the basis of the spirit of the present invention these modifications or improvements, belong to the scope of protection of present invention.
Claims (1)
1. it is a kind of improve tamato fruit in carotenoid content method, it is characterised in that methods described is molten using melatonin
Liquid carries out spraying process to green ripe stage Fructus Lycopersici esculenti；The carotenoid is alpha-carotene and beta-carotene；The melatonin solution
Concentration be 100 μM；The amount of spraying of the melatonin solution is 100ml/10 Fructus Lycopersici esculenti；Green ripe stage Fructus Lycopersici esculenti is taken off per 3 days
Melanocyte solution sprays process once.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510070026.8A CN104686215B (en) | 2015-02-10 | 2015-02-10 | Method for increasing content of carotenoids in tomato fruits |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510070026.8A CN104686215B (en) | 2015-02-10 | 2015-02-10 | Method for increasing content of carotenoids in tomato fruits |
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| CN104686215A CN104686215A (en) | 2015-06-10 |
| CN104686215B true CN104686215B (en) | 2017-05-03 |
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| CN201510070026.8A Expired - Fee Related CN104686215B (en) | 2015-02-10 | 2015-02-10 | Method for increasing content of carotenoids in tomato fruits |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766410B (en) * | 2016-03-16 | 2019-02-15 | 山东农业大学 | A method of promote greenhouse tomato to bear fruit and fruit growth using epiphysin |
| CN114698515B (en) * | 2022-05-09 | 2023-07-14 | 河北农业大学 | Method for promoting ripeness of tomato fruits and increasing sugar content of tomato fruits |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1963511A1 (en) * | 2005-12-23 | 2008-09-03 | Avestha Gengraine Technologies Pvt. Ltd. | Enhancement of carotenoids in plants |
| CN101979601A (en) * | 2010-09-21 | 2011-02-23 | 山东省农业科学院高新技术研究中心 | Method for improving carotenoid content of tomato |
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