CN104673503A - Method of simultaneously producing lutein and oil by utilizing microalgaes - Google Patents
Method of simultaneously producing lutein and oil by utilizing microalgaes Download PDFInfo
- Publication number
- CN104673503A CN104673503A CN201410586521.XA CN201410586521A CN104673503A CN 104673503 A CN104673503 A CN 104673503A CN 201410586521 A CN201410586521 A CN 201410586521A CN 104673503 A CN104673503 A CN 104673503A
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- China
- Prior art keywords
- extraction
- lutein
- grease
- xenthophylls
- oil
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C403/00—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone
- C07C403/24—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by six-membered non-aromatic rings, e.g. beta-carotene
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Fats And Perfumes (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Abstract
The invention discloses a method of simultaneously producing lutein and oil by utilizing microalgaes. The method comprises the following steps: (1) collecting microalgae cells; and (2) weighing the microalgae cells; (21) extracting the lutein; and (22) extracting the oil, namely arranging tests of exchanging extraction sequences of the lutein and the oil simultaneously to determine the optimized extraction sequence and level. According to the method disclosed by the invention, the microalgaes are crushed by adopting ultrasonic waves, the cavitation and the alcohol dissolution action of the ultrasonic waves have a good synergic effect, and the oil components in the cells are facilitated to spread by virtue a cell crusher; with methanol-dichloromethane as an extraction solvent, the extraction effect of the lutein is optimal, and in addition, the oil loss of the residual microalgae is small; by virtue of orthogonal optimization of the extraction process of the lutein, the extraction rate of the lutein under the optimal process condition is 94.59 percent and is increased by 1.43 times compared with that before optimization; the content of oil in the residual microalgae is 32.56 percent; and the fatty acid composition of the oil in original chlorella before and after extraction of the lutein are basically unchanged.
Description
Technical field
The invention belongs to technical field of microalga biology, relate to the comprehensive utilization technique of the high-valued meta-bolites of micro-algae, be specifically related to a kind of method utilizing micro-algae simultaneously to produce xenthophylls and grease.
Background technology
Micro-algae is that a class grows in water, of a great variety and distribution lower plant extremely widely.This kind of biology has efficient photosynthesis reactive system, can pass through the fixing of carbonic acid gas, be chemical energy, and be stored in light energy conversion in somatocyte with the organic form such as grease or starch.Along with the pressure of human society shortage of resources and environmental problem increasingly serious, the exploitation utilizing micro-algae to carry out biofuel and part fossil energy thereof to replace product, has become the focus of research at present.
Utilizing micro-algae to carry out production of biodiesel is a complicated systems engineering, contain multiple sport technique segment, but the subject matter faced at present is higher production cost, because the cost of micro-algae production biofuel is higher, in the industrialization production of microalgae biodiesel, also do not obtain the breakthrough of essence in the world.The extractive technique adopted traditionally, the time of usual separation and Extraction is long, and percentage extraction is low, there is a problem in traditional grease extraction simultaneously, namely the organic solvent of a large amount of toxic effects will be consumed in extraction process, this has not only made cost raising, and organic solvent easily damages HUMAN HEALTH and contaminate environment.
The one of xenthophylls carotenoid, is mainly present in fruits and vegetables.Current research shows, xenthophylls has extensively effective physiological function, effectively can eliminate free radical, and the diseases such as prevention cardiovascula arteriosclerosis, coronary heart disease and tumour, can improve the immunizing power of body; Prevent and treat the visual deterioration that causes depending on macular degeneration and blind etc.The commercial source of current xenthophylls is Potmarigold Calendula, and the growth cycle of Potmarigold Calendula is longer, and lutein content is low, and leaching process is comparatively loaded down with trivial details.
At present, most research mainly concentrates on the production of microalgae biodiesel or the production of micro-algae high value meta-bolites, does not also find research microalgae biodiesel and high added value meta-bolites combined.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of extracting method utilizing micro-algae to produce grease and xenthophylls, and the method is simple to operate, and the extraction yield of microalgae grease and xenthophylls is high.
In order to solve the problems of the technologies described above, the present invention realizes in the following manner:
Utilize micro-algae to produce a method for xenthophylls and grease simultaneously, comprise the following steps:
(1) collection of microalgae cell: in the later stage of microdisk electrode, utilizes centrifugal mode to collect microalgae cell xeraphium;
(2) the taking of microalgae cell: the microalgae cell xeraphium that the step (1) accurately taking 0.1g obtains;
(21) extraction of xenthophylls: for the microalgae cell obtained in step (2), add appropriate Extraction solvent, after vibration mixing, fragmentation is carried out to micro-algae sonicator, utilize centrifugal method to improve cell concn, control centrifuge RPMs 5500 ~ 8000r/min, centrifugation time is 5 ~ 10min, collect solution supernatant, constant volume, measure lutein content, whole process need carries out under lucifuge condition;
(22) extraction of grease: for the microalgae cell obtained in step (2), add the hydrochloric acid 6ml of 3 ~ 4mol/L, after vibration mixes, holding chamber is gentle and quiet puts 20 ~ 30min, then after placing boiling water bath 10 ~ 15min, proceed to-80 DEG C of freezing 15 ~ 25min of cryogenic refrigerator, repeat above-mentioned freeze thawing three times; Add chloroform and methanol extract liquid again, fully vibration mixes, centrifuge RPMs 5500 ~ 8000r/min, and centrifugation time is 5 ~ 10min, is proceeded to by lower floor's chloroform layer in new container and dries and obtain grease;
(3) determination of xenthophylls and grease sequence of extraction: above-mentioned steps (21) and the extraction of xenthophylls of step (22) and the order of the extraction of grease are exchanged respectively and carries out two groups of experiments, namely one group is first carried out the extraction of grease, measures fat content; Residue frond extracts xenthophylls again, measures lutein content; Another group experiment first extracts xenthophylls, measures lutein content; Residue frond extracts grease again, measures fat content.
In described step (22), the volume ratio of chloroform and methanol extract liquid is 1:1 ~ 10:1.
Extraction solvent in described step (21) be ethanol, ether, sherwood oil, acetone, normal hexane, methyl alcohol, methylene dichloride, normal hexane-ethanol, methyl alcohol-methylene dichloride, methyl alcohol-methylene dichloride and methyl alcohol-methylene dichloride one or more.
Claims (3)
1. utilize micro-algae to produce a method for xenthophylls and grease simultaneously, it is characterized in that: comprise the following steps:
(1) collection of microalgae cell: in the later stage of microdisk electrode, utilizes centrifugal mode to collect microalgae cell xeraphium;
(2) the taking of microalgae cell: the microalgae cell xeraphium that the step (1) accurately taking 0.1g obtains;
(21) extraction of xenthophylls: for the microalgae cell obtained in step (2), add appropriate Extraction solvent, after vibration mixing, fragmentation is carried out to micro-algae sonicator, utilize centrifugal method to improve cell concn, control centrifuge RPMs 5500 ~ 8000r/min, centrifugation time is 5 ~ 10min, collect solution supernatant, constant volume, measure lutein content, whole process need carries out under lucifuge condition;
(22) extraction of grease: for the microalgae cell obtained in step (2), add the hydrochloric acid 6ml of 3 ~ 4mol/L, after vibration mixes, holding chamber is gentle and quiet puts 20 ~ 30min, then after placing boiling water bath 10 ~ 15min, proceed to-80 DEG C of freezing 15 ~ 25min of cryogenic refrigerator, repeat above-mentioned freeze thawing three times; Add chloroform and methanol extract liquid again, fully vibration mixes, centrifuge RPMs 5500 ~ 8000r/min, and centrifugation time is 5 ~ 10min, is proceeded to by lower floor's chloroform layer in new container and dries and obtain grease;
(3) determination of xenthophylls and grease sequence of extraction: above-mentioned steps (21) and the extraction of xenthophylls of step (22) and the order of the extraction of grease are exchanged respectively and carries out two groups of experiments, namely one group is first carried out the extraction of grease, measures fat content; Residue frond extracts xenthophylls again, measures lutein content; Another group experiment first extracts xenthophylls, measures lutein content; Residue frond extracts grease again, measures fat content.
2. a kind of method utilizing micro-algae simultaneously to produce xenthophylls and grease according to claim 1, is characterized in that: in described step (22), the volume ratio of chloroform and methanol extract liquid is 1:1 ~ 10:1.
3. a kind of method utilizing micro-algae simultaneously to produce xenthophylls and grease according to claim 1, is characterized in that: the Extraction solvent in described step (21) be ethanol, ether, sherwood oil, acetone, normal hexane, methyl alcohol, methylene dichloride, normal hexane-ethanol, methyl alcohol-methylene dichloride, methyl alcohol-methylene dichloride and methyl alcohol-methylene dichloride one or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410586521.XA CN104673503A (en) | 2014-10-27 | 2014-10-27 | Method of simultaneously producing lutein and oil by utilizing microalgaes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410586521.XA CN104673503A (en) | 2014-10-27 | 2014-10-27 | Method of simultaneously producing lutein and oil by utilizing microalgaes |
Publications (1)
| Publication Number | Publication Date |
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| CN104673503A true CN104673503A (en) | 2015-06-03 |
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| CN201410586521.XA Pending CN104673503A (en) | 2014-10-27 | 2014-10-27 | Method of simultaneously producing lutein and oil by utilizing microalgaes |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106954853A (en) * | 2017-03-06 | 2017-07-18 | 广东药科大学 | A kind of grid algae water extract and preparation method and application |
| CN110903897A (en) * | 2019-12-27 | 2020-03-24 | 沈兰兰 | Method for extracting grease from chlamydomonas |
| CN115784956A (en) * | 2022-11-30 | 2023-03-14 | 西安交通大学 | Method for extracting lutein from microalgae by adopting surface active ionic liquid |
-
2014
- 2014-10-27 CN CN201410586521.XA patent/CN104673503A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106954853A (en) * | 2017-03-06 | 2017-07-18 | 广东药科大学 | A kind of grid algae water extract and preparation method and application |
| CN110903897A (en) * | 2019-12-27 | 2020-03-24 | 沈兰兰 | Method for extracting grease from chlamydomonas |
| CN115784956A (en) * | 2022-11-30 | 2023-03-14 | 西安交通大学 | Method for extracting lutein from microalgae by adopting surface active ionic liquid |
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Application publication date: 20150603 |