CN104650255A - Method for separating and purifying cordyceps polysaccharide contained in cordyceps militaris solid culture medium - Google Patents

Abstract

The invention provides a method for separating and purifying cordyceps polysaccharide contained in a cordyceps militaris solid culture medium. The method comprises the following steps of: (1) collecting residual cordyceps militaris solid culture mediums, crushing, screening through a screen with 80 meshes, and degreasing; (2) carrying out water bath extraction on a product obtained from the step (1), then removing impurities, and concentrating; (3) separating the product obtained from the step (2) through an ion exchange column, and eluting by using ethanol to obtain an eluent; (4) carrying out ultrafiltration for multiple times on the eluent collected from the step (3), and finally concentrating to obtain the cordyceps polysaccharide contained in the cordyceps militaris solid culture medium. The method for separating and purifying the cordyceps polysaccharide contained in the cordyceps militaris solid culture medium, which is provided by the invention, is easy to operate and high in extraction rate.

Description

The separation purification method of Cordyceps polysaccharide in Cordyceps militaris (L.) Link. solid medium

Technical field

The present invention relates to the separation purification method of Cordyceps polysaccharide in a kind of Cordyceps militaris (L.) Link. solid medium, belong to technical field of traditional Chinese medicines.

Background technology

Cordyceps militaris (L.) Link. is a kind of famous and precious medicinal fungi and high tonic, is that main raw material makes solid medium with rice, dried silkworm chrysalis meal, artificial culture Cordyccps-militaris-(L.)-link. Sporophore, but after sporophore gathers, the utilization of covering with mycelial solid deserted medium but rarely has report.The content of Cordyceps militaris (L.) Link. solid deserted medium cordycepin is about 5 ‰, total sugar content is 21.93%, protein remains reaches about 10% (front higher by 2.1% than cultivating), Cordyceps polysaccharide content is 12.86%, in addition containing effective constituents such as alkaloid, terpenoid, sterol, glycoside, phenols, enzyme, VITAMIN, Mn-SOD.

Summary of the invention

The object of the invention is to overcome the deficiencies in the prior art part, the separation purification method of Cordyceps polysaccharide in a kind of Cordyceps militaris (L.) Link. solid medium is provided.

In Cordyceps militaris (L.) Link. solid medium of the present invention, the separation purification method of Cordyceps polysaccharide, comprises the steps:

1) collect Cordyceps militaris (L.) Link. solid deserted medium, pulverized 80 mesh sieves, and carry out degreasing;

2) product step 1) obtained carries out water-bath lixiviate, then removes foreign material, concentrated;

3) by step 2) product that obtains is separated through ion exchange column, carries out wash-out obtain elutriant with ethanol;

4) elutriant step 3) collected carries out repeatedly ultrafiltration, finally concentrates and obtains Cordyceps polysaccharide in Cordyceps militaris (L.) Link. solid medium.

Preferably,

In described step 3), ion exchange column separation condition is as follows: material-water ratio 1:16, ion column pH 8; Elution flow rate is 1/2 seconds.

The separation purification method easy handling of Cordyceps polysaccharide in Cordyceps militaris (L.) Link. solid medium of the present invention, extraction yield is high.

Embodiment

Embodiment 1

In the Cordyceps militaris (L.) Link. solid medium of the present embodiment, the separation purification method of Cordyceps polysaccharide, comprises the steps:

1) collect Cordyceps militaris (L.) Link. solid deserted medium, pulverized 80 mesh sieves, and carry out degreasing;

2) product step 1) obtained carries out water-bath lixiviate, then removes foreign material, concentrated;

3) by step 2) product that obtains is separated through ion exchange column, and carry out wash-out with ethanol and obtain elutriant, ion exchange column separation condition is as follows: material-water ratio 1:16, ion column pH 8; Elution flow rate is 1/2 seconds.

4) elutriant step 3) collected carries out repeatedly ultrafiltration, finally concentrates and obtains Cordyceps polysaccharide in Cordyceps militaris (L.) Link. solid medium.

Claims (2)

1. the separation purification method of Cordyceps polysaccharide in Cordyceps militaris (L.) Link. solid medium, is characterized in that, comprise the steps:

1) collect Cordyceps militaris (L.) Link. solid deserted medium, pulverized 80 mesh sieves, and carry out degreasing;

2) product step 1) obtained carries out water-bath lixiviate, then removes foreign material, concentrated;

3) by step 2) product that obtains is separated through ion exchange column, carries out wash-out obtain elutriant with ethanol;

4) elutriant step 3) collected carries out repeatedly ultrafiltration, finally concentrates and obtains Cordyceps polysaccharide in Cordyceps militaris (L.) Link. solid medium.

2. the separation purification method of Cordyceps polysaccharide in Cordyceps militaris (L.) Link. solid medium according to claim 1, it is characterized in that, in described step 3), ion exchange column separation condition is as follows: material-water ratio 1:16, ion column pH 8; Elution flow rate is 1/2 seconds.