CN104623731A - Bone repair material - Google Patents
Bone repair material Download PDFInfo
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- CN104623731A CN104623731A CN201310557728.XA CN201310557728A CN104623731A CN 104623731 A CN104623731 A CN 104623731A CN 201310557728 A CN201310557728 A CN 201310557728A CN 104623731 A CN104623731 A CN 104623731A
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Abstract
The invention relates to a bone repair material for bone defect. Allogenic tendon collagen fiber and allogeneic bone particles are mixed in a ratio of 1:5 to form a component A, a colorless clarified viscous sodium hyaluronate solution with a pH value of 6 and a concentration of 15mg/ml is taken as a component B, and the component A and the component B are blended according to a proportion of 1g:2ml into a plasticine-like allogeneic bone paste. Bone graft filler metal granules are provided with bone graft holes accommodating grafted bone paste, all the bone graft holes are in cut-through connection, and intercommunicated micropores are distributed on the outer surfaces of the granules and the inner surfaces of the bone graft holes. The allogeneic bone paste and the bone graft filler metal granules are blended in a ratio of 10:1 for bone defect filling and partial substitution of autogenous bone or allogeneic bone filler in the form of occupation, and during patient rehabilitation, bone cells and fine blood vessels can fill bone graft. Metal particles can grow inside to form fused embedded bioactive bone restorations.
Description
Technical field
The present invention relates to a kind of bone renovating material, specifically, relate to a kind of bone renovating material made for bone grafting filling metallic particles for overlay and bone mud.
Background technology
At present, the medical repair composite material clinically for bone injury has a variety of, as polymeric material, metal material, various bioactive materials and pottery etc.But, although the application of these composition for repairing is very wide, because these composites are placed among human body for a long time, its biocompatibility and biological degradability not ideal, this shortcoming is never well solved.The reparation of Cranial defect is the surgical stubborn problem of long-standing problem.
Bone mud, as a kind of new type bone graft materials, has good self-bone grafting and bone conduction effect.It can be filled in Cranial defect place according to the size of reality or shape, can be filled between fracture two broken ends of fractured bone after moulding.It also easily mixes with autologous bone or bone marrow and can keep its integrity.The above-mentioned advantage of bone mud makes it be subject to the extensive concern of research worker.
Bone mud can be preserved in a reservoir, very convenient.Abroad, the especially U.S., bone mud has been widely used in clinical.But also there are some problems in existing bone mud.In existing bone mud preparation method, be mostly adopt glycerol or gelatin as moulding dose, because glycerol itself has nephrotoxicity, make existing bone mud can not more than 2ml/kg at the application quantity of human body, and use the intensity of gelatin bone mud to be difficult to reach requirement, so bone mud application is in vivo restricted.
Summary of the invention
The object of this invention is to provide a kind of biological activity and biological degradability is good, the repairing bone defect allograph bone repair materials that do not have nephrotoxicity, also provide its preparation method simultaneously.
In order to achieve the above object, the present invention is by the following technical solutions: a kind of repairing bone defect material, be characterized in: mixed by Heterogeneous tendon collagen fiber and allograph bone granule and form component A, the mixing ratio of Heterogeneous tendon collagen fiber and allograph bone granule is 1:5; The sodium hyaluronate solution being 15mg/ml by achromatism and clarity thickness, pH value 6, concentration is B component; Component A mixes with B component the allosome bone mud be in harmonious proportion in rubber pureed in the ratio of 1g:2ml; By allosome bone mud and bone grafting filling metallic particles 10:1 mix and blend, fill for Cranial defect, with the autologous bone of occupy-place form Some substitute or allograph bone implant, in Rehabilitation process, osteocyte and capillary vessel are filled to bone grafting; Growing in metal particle bodies inside, can be formed and merge Embedded biological activity bone repair.
Metallic granular body for bone-grafting filling, it is characterized in that, this granule shape is spheroid, cylinder, polyhedron, spirillum, reticulate body and other similar space geometric froms, this granule overall dimensions is 2-15mm, is filled in Cranial defect position during use by after the bone mud mix and blend of the metallic granular body for bone-grafting filling of right quantity and allograph bone; Metallic granular body for bone-grafting filling is provided with the bone grafting hole holding and implant bone mud, and bone grafting hole is extended inward by the outer surface of metallic granular body for bone-grafting filling, bone grafting aperture 1-10mm, mutual through connected between each bone grafting hole.Metal solid partial outer face and the bone grafting internal surface of hole of this granule are distributed with the micropore be interconnected, and the aperture of this micropore is 50um-900um; Metallic granular body for bone-grafting filling is characterised in that it adopts medical metal material to make, and described medical metal material comprises titanium or titanium alloy, cobalt alloy, rustless steel and magnesium alloy; Metallic granular body for bone-grafting filling surface all or locally has hydroxyapatite coating layer, and this hydroxyapatite coating layer has the function of induction bone cell growth.
The preparation method of described repairing bone defect allosome bone mud, comprises the following steps successively:
A, sterile working, get Heterogeneous tendon, after tendon is air-dry, is ground into the flocculence tendon collagen fiber that diameter is 0.05-0.2mm, sterilizes under 25kGy gamma ray in tissue pulverizer, for subsequent use;
B, sterile working, allosome cortex bone is pulverized, and cross 100 eye mesh screens, collection cut size is less than the allograph bone granule of 0.147mm, and sterile deionized water washs 3 times, 75% washing with alcohol 3 times, and eccentric cleaning collects allograph bone granule, air-dry, for subsequent use;
C, sterile working, the ratio that the allograph bone granule that tendon collagen fiber a step obtained and b step obtain is 1/ (2-8) with weight ratio is mixed into component A, for subsequent use;
D, sterile working, by the B component of component A and outsourcing in 1g:(2-3.5ml) ratio mix and be in harmonious proportion after in rubber pureed, make allosome bone mud, preserve in 1-20 DEG C of refrigerator.
The processing method of described metallic granular body for bone-grafting filling is: forging, casting, machine cut boring, electric discharge machine-shaping etc.; Other processing method also comprises the while of adopting the technology such as the technology, particularly laser such as laser or high-power electron beam rapid shaping technique, high temperature sintering, chemical attack, electrochemical deposition or high-power electron beam rapid shaping technique, high temperature sintering, chemical attack, electrochemical deposition except can making the metal solid of metallic granular body for bone-grafting filling can also generate at this solid object surface and bone grafting internal surface of hole the micropore be interconnected; Hydroxyapatite coating layer is then obtained by pyrolytic coating or electrochemical deposition.
The present invention adopts the component A of form of mixtures and plays the B component mixing of firming agent form, can on-the-spotly in use mix, the mixing of the component A of powdery and liquid B component allosome bone mud can be made to mix evenly, make it that there is good biological activity and biological degradability, and remained by complete due to the BMP in allograph bone granule, made bone mud is also possessed good self-bone grafting and bone conduction effect.Simultaneously, swelling, the crosslinked action of B component to component A also make the composite of bone mud can carry out as required as plasticine to plant in Cranial defect place after random-shaping is combined with bone grafting filling metallic particles for overlay, can closely agree with defect and solidify voluntarily after moulding, bond strength is high, easy to use, reach the object of faster and better repairing bone defect, this technical scheme makes to prepare allograph bone repair materials to be had good biological activity and biological degradability and not to have nephrotoxicity, possess good self-bone grafting and bone conduction effect, and easy to use; Meanwhile, use the autologous bone of metallic granular body for bone-grafting filling Some substitute and allograph bone, greatly can reduce the financial burden of patient and overcome the quantitative difficulties and insufficiency obtaining autologous bone and allograph bone to a certain extent; Can reach after bone mud fusion growth in sclerotin during the convalescence after Miles operation around defect and metallic granular body for bone-grafting filling bone grafting hole is integral and prevent this metallic granular body for bone-grafting filling from the effect loosened occurring long-term effectively.So the present invention has a wide range of applications in bone defect healing field.
Detailed description of the invention
Embodiment
Prepare a kind of repairing bone defect repair materials, comprise the following steps successively:
A, sterile working, get rabbit Heterogeneous tendon.Peel off clean by tendon surface fascia, cutting tendon is the fritter of 0.3cm × 0.3cm, with 75% ethanol rinse 3 times, and natural air drying tendon fritter under room temperature condition.In tissue pulverizer, air-dry tendon fritter is ground into the flocculence collagen fiber that diameter is 0.1mm, collagen fiber of accurately weighing, the some parts of 0.1g/ part subpackage, sterilize under 25kGy gamma ray, for subsequent use;
B, sterile working, get rabbit allosome cortical bone.In tissue pulverizer, allosome cortex bone meal is broken into fine powder.Sieved 100 eye mesh screens, collection cut size is less than the fine powder of 0.147mm, 75% washing with alcohol 3 times, and eccentric cleaning collects bone meal, air-dry, bone meal granule of accurately weighing, and the some parts of 0.6g/ part subpackage are for subsequent use;
C, sterile working, the allograph bone granule that tendon collagen fiber a step obtained and b step obtain is that the ratio of 1:4 is mixed into component A with weight ratio, for subsequent use;
D, sterile working, be that the B component of 10mg/ml mixes in the ratio of 1g:2.5ml and is in harmonious proportion after in rubber pureed by component A and outsourcing concentration, be prepared into the allosome bone mud of repairing bone defect, preserve in-20 DEG C of refrigerators;
E, by allosome bone mud and bone grafting filling metallic particles 10:1 mix and blend, to fill for Cranial defect.
Metallic granular body for bone-grafting filling of the present invention is placed in Cranial defect place after using Shi doctor that the bone grafting filling metallic particles for overlay of suitable size and shape can be selected as required to mix with bone mud and is fixed on bone bed to obtain the early stage stable of this bone grafting repair materials by suppressing; Metallic granular body for bone-grafting filling is in position provided with the bone grafting hole of accommodation bone mud for filling the broken osseous granules of autologous or allosome, the outer surface of this external metal solid part and the inner surface in bone grafting hole are distributed with the micropore be interconnected, after Operation until the infiltration of outside osseous tissue is grown into micropore and integral with the bone mud fusion growth of filling in bone grafting hole after can reach and prevent this bone grafting filling renovation material from loosening object occurring long-term effectively.
Claims (9)
1. a repairing bone defect bone renovating material, it is characterized in that being mixed by Heterogeneous tendon collagen fiber and allograph bone granule forming component A, the mixing ratio of Heterogeneous tendon collagen fiber and allograph bone granule is 1:5; The sodium hyaluronate solution being 15mg/ml by achromatism and clarity thickness, pH value 6, concentration is B component; Component A mixes with B component the allosome bone mud be in harmonious proportion in rubber pureed in the ratio of 1g:2ml; Metallic granular body for bone-grafting filling, be provided with the bone grafting hole holding and implant bone mud, each bone grafting hole is through to be connected, outer surface and the bone grafting internal surface of hole of granule are distributed with the micropore be interconnected, by allosome bone mud and bone grafting filling metallic particles 10:1 mix and blend, fill for Cranial defect, with the autologous bone of occupy-place form Some substitute or allograph bone implant.
2. repairing bone defect bone renovating material according to claim 1, is characterized in that described tendon collagen fiber take from Heterogeneous tendon, and tendon stirs and pulverizes after air-dry in tissue pulverizer, forms the flocculence collagen fiber group that diameter is 0.1mm.
3. repairing bone defect bone renovating material according to claim 1, is characterized in that described allograph bone granule is broken into by allosome cortex bone meal the fine powder that particle diameter is less than 0.147mm (being namely equivalent to 100 mesh sieves).
4. the metallic granular body for bone-grafting filling of repairing bone defect bone renovating material according to claim 1, it is characterized in that, metallic granular body for bone-grafting filling, it is characterized in that, this granule shape is spheroid, cylinder, polyhedron, spirillum, reticulate body and other similar space geometric froms, and this granule overall dimensions is 2-15mm.
5. metallic granular body for bone-grafting filling according to claim 4, is characterized in that, metallic granular body for bone-grafting filling is provided with the bone grafting hole holding and implant bone mud, and bone grafting hole is extended inward by the outer surface of metallic granular body for bone-grafting filling, bone grafting aperture 1-10mm.
6., according to claim 4 and metallic granular body for bone-grafting filling according to claim 5, it is characterized in that, mutual through connected between each bone grafting hole.
7. according to claim 4 and metallic granular body for bone-grafting filling according to claim 5, it is characterized in that, metal solid partial outer face and the bone grafting internal surface of hole of this metallic granular body for bone-grafting filling are distributed with the micropore be interconnected, and the aperture of this micropore is 100um-900um.
8. according to claim 4 and metallic granular body for bone-grafting filling according to claim 5, it is characterized in that, the inner surface of metallic granular body for bone-grafting filling outer surface and value bone hole has hydroxyapatite coating layer.
9. metallic granular body for bone-grafting filling according to claim 4, is characterized in that, this metallic granular body for bone-grafting filling adopts medical metal to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310557728.XA CN104623731A (en) | 2013-11-07 | 2013-11-07 | Bone repair material |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310557728.XA CN104623731A (en) | 2013-11-07 | 2013-11-07 | Bone repair material |
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| CN104623731A true CN104623731A (en) | 2015-05-20 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108114316A (en) * | 2017-12-22 | 2018-06-05 | 北京奥精医药科技有限公司 | A kind of ceramic base dentistry bone meal and preparation method thereof |
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2013
- 2013-11-07 CN CN201310557728.XA patent/CN104623731A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108114316A (en) * | 2017-12-22 | 2018-06-05 | 北京奥精医药科技有限公司 | A kind of ceramic base dentistry bone meal and preparation method thereof |
| CN108114316B (en) * | 2017-12-22 | 2021-07-02 | 奥精医疗科技股份有限公司 | Ceramic-based dental bone powder and preparation method thereof |
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Application publication date: 20150520 |
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