CN104800884A - Decalcified human dentinal matrix material and preparation method thereof - Google Patents
Decalcified human dentinal matrix material and preparation method thereof Download PDFInfo
- Publication number
- CN104800884A CN104800884A CN201510089391.3A CN201510089391A CN104800884A CN 104800884 A CN104800884 A CN 104800884A CN 201510089391 A CN201510089391 A CN 201510089391A CN 104800884 A CN104800884 A CN 104800884A
- Authority
- CN
- China
- Prior art keywords
- chitosan
- bone
- matrix material
- preparation
- matrix
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention belongs to the technical field of medicines, and relates to a novel decalcified human dentinal matrix porous scaffold and a preparation method thereof. The porous scaffold comprises a decalcified human dentinal matrix, chitosan and a silk fibroin compound, wherein the decalcified human dentinal matrix and chitosan/silk fibroin are cross-linked in different content ratios. Porous scaffolds which have different shapes and different porosities and are required by bone formation can be prepared from the decalcified human dentinal matrix, and the decalcified human dentinal matrix is applicable to treatment of diseases such as bone defects, bone nonunion, bone tissue atrophy, fractures, undesirable bone healing and the like so as to play roles in inducing osteogenesis, promoting bone repairs and promoting wound convergence. The preparation technology is stable and easy to control.
Description
Technical field
The invention belongs to medical art, relate to a kind of decalcifying tooth matrix gel and preparation method.This gel can be prepared into difform porous support, can be well moulding to teeth matrix, the treatment of teeth matrix is applicable to better diseases such as Cranial defect, bone does not connect, osseous tissue atrophy, fracture, knitting be bad, and its induction osteogenesis of the performance of continuous and effective, effect of promoting Bone Defect Repari, promoting wound convergence.
Background technology
Nineteen sixty-five, the Urist of the U.S. finds that decalcified bone matrix can induce the formation of bone and cartilage, and the self-bone grafting repair theory that to define with bone morphogenetic protein (BMP) be core.After this, develop a series of osteogenic materials deriving from organism both at home and abroad, mainly contain autogenous bone material and homogeneous allogenic bone.Autologous bone, without rejection, has good bone-inducting active, is the goldstandard of bone-grafting material.But the transplanting of autologous bone can bring secondary insult to patient, also can cause the afunction for bone district, autologous bone-specific drug Cranial defect has limitation to defect treatment in a big way in addition, is also subject to the restriction at patient's body constitution and age; And although homogeneous allogenic bone is easy to use, because Immunogenicity is easier to cause rejection thus causes Endodontic failure, often face the problem of second operation.Along with to BMP progress of research, find that BMP can induced ectopic skeletonization.Restructuring rh-BMP has the effect of self-bone grafting, but is easy to diffusion dilution and being easily degraded by proteases in vivo, is difficult to play ossification in vivo for a long time, does not also find suitable carrier at present, cause its clinical practice limited.Decalcifying tooth matrix material is a kind of natural complex containing BMP, collagen protein, hydroxyapatite, has tool bone-inducting active, and layering is degraded, and can ensure various body constitution crowd high-quality skeletonization.When after material implanting to human body, bone-inducing factor BMP contained by it to be combined with signaling molecule by the receptor in mesenchymal cell and to carry out signal transduction, and send gene regulatory signal in local, implantation region, inducing mesenchymal cell differentiation becomes chondrocyte and osteocyte.After through cartilage matrix precipitation, blood vessel is grown into, and bone marrow and medullary cavity such as to be differentiated to form at the process, finally induce new bone formation.
Chitosan is chitin deacetylase product, is a kind of poly-amino cationic polysaccharide, has good biocompatibility, degradable, have no side effect and non-immunogenicity.Can by enzyme hydrolysis after chitosan implant into body, hydrolyzate non-immunogenicity, its structure is similar to the glycosaminoglycans in extracellular matrix, and can not form fibrous capsule in vivo, foreign body reaction is low.Plasticity is strong, possesses good swelling behavior, can be prepared into the porous support adapting to different bone defect healing requirement.Chitosan itself has reinforced immunological function, accelerates wound healing, promotes adhesion and the proliferation and differentiation of mesenchymal cell.
Fibroin albumen is a kind of fibrous protein, has porous and higher water absorption, the high and degradable of biocompatibility.Compound bio engineering rack prepared by fibroin albumen and chitosan, can improve the toughness of chitosan and the water absorption of material, improves compressive property and the hydrophilic of material.
The existing process suitable by the method for science of decalcifying tooth matrix material, obtains and can induce osteogenesis, promote osseous tissue to be formed and repair a kind of novel bone induction material.The treatment of this material to diseases such as bone does not connect, osseous tissue atrophy absorption, malunion of fracture and large-area Cranial defect has good effect.
Summary of the invention
An object of the present invention proposes a kind of decalcifying tooth matrix material, teeth matrix the is filled into sick positions such as Cranial defect, bone does not connect, osseous tissue atrophy, fracture, knitting be bad, the effect play its induction osteogenesis, promoting Bone Defect Repari, promote wound convergence.
Two of object of the present invention is the preparation methoies proposing above-mentioned decalcifying tooth matrix material.
One of goal of the invention of the present invention is cross-linked realization by people's teeth matrix, chitosan and fibroin albumen.This decalcifying tooth matrix material comprises people's teeth matrix of chitosan and chitosan mass 30% ~ 70% and the fibroin albumen of 10% ~ 20%, and the lyophilizing after EDC, NHS are crosslinked of described decalcifying tooth matrix material is prepared from.
Described teeth matrix is the bone renovating material prepared with discarded people's tooth, prepares for adopting following methods:
(1) the people's former tooth material mechanical means through 5% hypochlorite disinfectant removes the bad tooth body of enamel, cementum and dental caries etc., retains dentin, pulverizes as 10-140 order granule;
(2) by after the graininess dentin removal of impurity after pulverizing, the 1:1 chloroform methanol putting into 5 times of volumes soaks 30 minutes;
(3) the 0.6 M HCl that 5 times of volumes are put in clean flowing water rinsing after 4 hours soaks 18 ~ 24 hours;
(4) sterile deionized water obtains teeth matrix after rinsing rinsing repeatedly.
Two of goal of the invention of the present invention is achieved through the following technical solutions, and a kind of preparation method preparing above-mentioned tooth decalcifying tooth matrix material, it is characterized in that, the method comprises the steps:
(1) chitosan is dissolved in 2% acetic acid solution, makes 3% chitosan solution
(2) in above-mentioned chitosan solution, add chitosan mass 30% ~ 70% teeth matrix, mechanical activation comminution obtains chitosan/teeth matrix mixing nano pulp;
(3) take a certain amount of silk fibroin powder and be dissolved in CaCl in 80 DEG C
2: C
2h
5oH:H
2in O solution (concentration ratio 1:2:8), dialyse 24 h, cryoconcentration, the silk fibroin protein solution of preparation 1%;
(4) vigorous stirring limit in limit adds the silk fibroin protein solution of chitosan mass 10% ~ 20%, prepares mixed slurry, pours in mould;
(5) freezing 2 ~ 5 days in-80 ~-20 DEG C, lyophilizing obtains just teeth matrix porous support processed;
(6) first porous support processed is immersed 24 h in the alcoholic solution of 2%NaOH, washed with de-ionized water is to neutral, and immersing containing EDC, NHS(concentration ratio is 2:1) 95% ethanol water in, crosslinkedly at 4 DEG C to spend the night.Deionized water rinsing is to no solvent residue;
(7) in-80 ~-20 DEG C of freezing 48 h, lyophilizing obtains decalcifying tooth matrix porous support;
(8) cut, vacuum packaging, sterilizing, virus of going out, obtained decalcifying tooth matrix finished material.
Described chitosan is commercially available medical chitosan, and its deacetylation is 80% ~ 95%;
Described fibroin albumen is commercially available medical fibroin protein powder, and its mean diameter is 2 μm;
Described decalcifying tooth matrix material is porous support, and its porosity is 60% ~ 90%.
The present invention utilizes EDC, NHS dexterously, and by having, the decalcifying tooth matrix of bone-inducting active is crosslinked to be fixed on chitosan/fibroin albumen carrier, utilize superior swelling of carrier and biodegradable characteristic, strengthen the plasticity of decalcifying tooth matrix, increase the dosage of decalcifying tooth matrix osseous tissue damage local, and providing temporary support for the growth of cell and cambium, inducing cell realizes directed differentiation and the tissue regeneration of local better.
Dentoidin sponginum of the present invention has following advantage:
1, this decalcifying tooth matrix material is in damaged part by teeth matrix and chitosan/fibroin albumen combined effect, people's teeth matrix contains bone-inducing factor BMP, can inducing mesenchymal cell differentiation be osteoblast and chondrocyte, chitosan/fibroin albumen can promote the adhesion of mesenchymal cell and somatomedin, and therefore described decalcifying tooth matrix material better can promote the reparation of damaged bony tissues.
2, the decalcifying tooth matrix material that prepared by the present invention is the porous support materials that can meet bone growth demand, this material porosity is 65 ~ 90%, for porous is through, miniature three-dimensional support is formed in implantation region, be conducive to cell in blood and somatomedin is assembled in its surface adsorption, thus guide and induce the regeneration of damaged bony tissues.
3, during material implantable bone defective region, chitosan/fibroin albumen has hydrophilic, can contact rapidly and the somatomedin adhered in blood and extracellular matrix and cell, make teeth matrix relatively be confined to damaged part, effectively improves damage local bone substrate dosage.
4, teeth matrix itself has slow-release function, be fixed on after on chitosan/fibroin albumen carrier through crosslinked, secondary slow release will be formed further, along with chitosan/fibroin albumen carrier is degraded and progressively some teeth matrixes of slow releasing, can more among a small circle, the longer time maintains effective teeth matrix dosage.
5, this decalcifying tooth matrix Biocompatibility is good, has no side effect, can thoroughly biodegradation, and catabolite non-immunogenicity, biological safety is high.
6, this decalcifying tooth matrix material is taken packaging apart and can be used, and can be prepared into and adapt to different Bone Defect Repari regions required form, facilitate clinical practice; The advantages such as preparation technology is simple, and stability is high, and raw material sources is abundant, cost is low.
Claims (4)
1. a decalcifying tooth matrix material, is characterized in that, this people's teeth matrix material comprises people's teeth matrix of chitosan and chitosan mass 30% ~ 70% and the fibroin albumen of 10% ~ 20%.
2. a decalcifying tooth matrix material, its preparation method is:
(1) chitosan is dissolved in 2% acetic acid solution, makes 1 ~ 3% collagen-chitosan solution;
(2) in above-mentioned chitosan solution, add decalcifying tooth matrix, mechanical activation comminution makes slurry;
(3) add silk fibroin protein solution mix homogeneously while stirring, pour pre-freeze in mould into;
(4) freezing 2 ~ 5 days in-80 ~-20 DEG C, lyophilizing obtains just porous support materials processed;
(5) crosslinked: take out after lyophilizing immerse containing 1-ethyl-3-(DimethylAminopropyl)-carbodiimides (EDC),
N-hydroxysuccinimide (NHS) is cross-linked 24h, lyophilizing;
(6) cut, virus of going out, vacuum packaging, the steps such as sterilizing obtain Dentoidin sponginum finished product.
3. a kind of decalcifying tooth matrix material according to claim 1 and 2 and preparation method thereof, is characterized in that, described deacetylating degree of chitosan is 80% ~ 95%.
4. a kind of decalcifying tooth matrix material according to claim 1 and 2 and preparation method thereof, is characterized in that, described decalcifying tooth matrix material is porous support, and porosity is 65% ~ 90%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510089391.3A CN104800884A (en) | 2015-02-27 | 2015-02-27 | Decalcified human dentinal matrix material and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510089391.3A CN104800884A (en) | 2015-02-27 | 2015-02-27 | Decalcified human dentinal matrix material and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104800884A true CN104800884A (en) | 2015-07-29 |
Family
ID=53686358
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510089391.3A Pending CN104800884A (en) | 2015-02-27 | 2015-02-27 | Decalcified human dentinal matrix material and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104800884A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110338944A (en) * | 2018-08-20 | 2019-10-18 | 郭建群 | Permanent teeth extract is preparing application and lower jaw packing material in lower jaw packing material |
| CN110420354A (en) * | 2019-09-03 | 2019-11-08 | 杨兴华 | A kind of enhanced fibroin albumen artificial jaw bone carrier bracket and preparation method thereof |
| CN116942911A (en) * | 2023-07-21 | 2023-10-27 | 北京科健生物技术有限公司 | Composite bone repair material and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004022120A1 (en) * | 2002-09-04 | 2004-03-18 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Compositions comprising bone marrow cells, demineralized bone matrix and rtg polymers for use in the induction of bone and cartilage formation |
| CN102309523A (en) * | 2011-05-18 | 2012-01-11 | 深圳市光明创博生物制品发展有限公司 | Dentoidin sponginum and preparation method thereof |
-
2015
- 2015-02-27 CN CN201510089391.3A patent/CN104800884A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004022120A1 (en) * | 2002-09-04 | 2004-03-18 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Compositions comprising bone marrow cells, demineralized bone matrix and rtg polymers for use in the induction of bone and cartilage formation |
| CN102309523A (en) * | 2011-05-18 | 2012-01-11 | 深圳市光明创博生物制品发展有限公司 | Dentoidin sponginum and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 卢贤欣 等: ""脱钙牙基质与成骨细胞的生物相容性"", 《中国组织工程研究与临床康复》 * |
| 叶鹏 等: ""丝素/壳聚糖/纳米羟基磷灰石构建的骨组织工程支架"", 《中国组织工程研究》 * |
| 李佳楠 等: ""脱钙人牙基质复合胶原骨修复材料的制备与性能研究"", 《四川大学学报（自然科学版）》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110338944A (en) * | 2018-08-20 | 2019-10-18 | 郭建群 | Permanent teeth extract is preparing application and lower jaw packing material in lower jaw packing material |
| CN110420354A (en) * | 2019-09-03 | 2019-11-08 | 杨兴华 | A kind of enhanced fibroin albumen artificial jaw bone carrier bracket and preparation method thereof |
| CN116942911A (en) * | 2023-07-21 | 2023-10-27 | 北京科健生物技术有限公司 | Composite bone repair material and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhang et al. | Biocompatiable silk fibroin/carboxymethyl chitosan/strontium substituted hydroxyapatite/cellulose nanocrystal composite scaffolds for bone tissue engineering | |
| EP3338819B1 (en) | Injectable composite material for bone repair, and preparation method thereof | |
| Florczyk et al. | Evaluation of three‐dimensional porous chitosan–alginate scaffolds in rat calvarial defects for bone regeneration applications | |
| CN101564553B (en) | Humanization active forging bone and preparation method thereof | |
| CN102294049B (en) | Bioactive glass and chitosan composite bone repair material and preparation method and application thereof | |
| Nie et al. | Nano-hydroxyapatite mineralized silk fibroin porous scaffold for tooth extraction site preservation | |
| CN106806943A (en) | Formed in situ Injectable bio-active composite aquogel and its preparation method and application | |
| CN104906637A (en) | Injectable-porous-drug loaded polymethyl methacrylate-based composite scaffold bone transplant material and preparation method thereof | |
| Song et al. | Bone morphogenetic protein-2 sustained delivery by hydrogels with microspheres repairs rabbit mandibular defects | |
| CN111773432A (en) | Magnesium-based amorphous-calcium phosphate/calcium silicate composite filler and preparation and application thereof | |
| CN104800884A (en) | Decalcified human dentinal matrix material and preparation method thereof | |
| Tang et al. | Hard tissue compatibility of natural hydroxyapatite/chitosan composite | |
| KR101777427B1 (en) | Biomaterials containing calcium phosphate | |
| KR101885896B1 (en) | Natural bone regeneration material containing minerals derived from human bone | |
| CN113694254A (en) | Bone repair material, preparation method and application thereof | |
| CN112076350B (en) | Biomimetic mineralized hydrogel with nano-micron composite structure and high mineral density as well as preparation method and application thereof | |
| Yuan et al. | Experimental study of natural hydroxyapatite/chitosan composite on reconstructing bone defects | |
| CN102309523B (en) | Dentoidin sponginum and preparation method thereof | |
| CN112999420A (en) | Plastic bone sponge for orthopedic filling and preparation method thereof | |
| CN107496988B (en) | Freeze-dried tooth bone powder material and preparation method thereof | |
| Majumdar et al. | Bioactive glass: soft tissue reparative and regenerative applications | |
| Ma et al. | Optimization of a concentrated growth factor/mesoporous bioactive glass composite scaffold and its application in rabbit mandible defect regeneration | |
| Liu et al. | Preparation of fish decalcified bone matrix and its bone repair effect in rats | |
| CN111973810B (en) | Porous hollow tubular material for regeneration, repair and reconstruction of large-section bone defects of limbs and preparation method thereof | |
| Sun et al. | Highly active biological dermal acellular tissue scaffold composite with human bone powder for bone regeneration |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150729 |