CN104622999A - Process for extracting total triterpenes from silverweed cinquefoil roots - Google Patents
Process for extracting total triterpenes from silverweed cinquefoil roots Download PDFInfo
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- CN104622999A CN104622999A CN201510015721.4A CN201510015721A CN104622999A CN 104622999 A CN104622999 A CN 104622999A CN 201510015721 A CN201510015721 A CN 201510015721A CN 104622999 A CN104622999 A CN 104622999A
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- extract
- radix potentillae
- potentillae anserinae
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- 150000003648 triterpenes Chemical class 0.000 title claims abstract description 24
- 244000308495 Potentilla anserina Species 0.000 title abstract description 8
- 238000000034 method Methods 0.000 title abstract description 6
- 235000009728 Potentilla anserina subsp anserina Nutrition 0.000 title abstract 6
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims abstract description 80
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 57
- 239000000284 extract Substances 0.000 claims abstract description 37
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000000843 powder Substances 0.000 claims abstract description 26
- 239000000287 crude extract Substances 0.000 claims abstract description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000001035 drying Methods 0.000 claims abstract description 17
- 238000001914 filtration Methods 0.000 claims abstract description 10
- 238000010298 pulverizing process Methods 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 9
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 7
- 239000012535 impurity Substances 0.000 claims abstract description 6
- 241001441970 Anserinae Species 0.000 claims description 60
- 238000000605 extraction Methods 0.000 claims description 45
- 239000006166 lysate Substances 0.000 claims description 30
- 229960004756 ethanol Drugs 0.000 claims description 21
- 230000001476 alcoholic effect Effects 0.000 claims description 16
- 230000009514 concussion Effects 0.000 claims description 16
- 239000000706 filtrate Substances 0.000 claims description 16
- 229960000935 dehydrated alcohol Drugs 0.000 claims description 10
- 238000010828 elution Methods 0.000 claims description 10
- 238000004062 sedimentation Methods 0.000 claims description 9
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 6
- 239000011347 resin Substances 0.000 claims description 6
- 229920005989 resin Polymers 0.000 claims description 6
- 230000000274 adsorptive effect Effects 0.000 claims description 5
- 229910052799 carbon Inorganic materials 0.000 claims description 5
- 238000004042 decolorization Methods 0.000 claims description 5
- 239000003480 eluent Substances 0.000 claims description 5
- 238000007689 inspection Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000004090 dissolution Methods 0.000 abstract description 4
- 238000002156 mixing Methods 0.000 abstract 2
- 239000002244 precipitate Substances 0.000 abstract 2
- 238000007873 sieving Methods 0.000 abstract 1
- 229920002472 Starch Polymers 0.000 description 4
- 235000019698 starch Nutrition 0.000 description 4
- 239000008107 starch Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 235000016594 Potentilla anserina Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- -1 triterpenoid compound Chemical class 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 1
- 241001092489 Potentilla Species 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000003014 reinforcing effect Effects 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
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- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
The invention relates to a process for extracting total triterpenes from silverweed cinquefoil roots. The process comprises the following steps: (1) pulverizing the silverweed cinquefoil roots, and sieving to obtain silverweed cinquefoil root powder; (2) extracting the silverweed cinquefoil root powder with the solution of ethanol to obtain an extract, filtering the extract, and concentrating by reducing pressure to obtain an concrete; (3) adding water to precipitate the concrete, stirring, standing at room temperature, and filtering to obtain a precipitate; (4) adding ethanol absolute to dissolve the precipitate to obtain a solution of dissolution, adding activated carbon into the solution of dissolution to decolor and remove impurities, recovering ethanol, and drying the solution of dissolution to constant weight by reducing pressure to obtain a crude extract; (5) dissolving the crude extract, adding ethyl ether to extract, standing at room temperature to layer, extracting repeatedly for 3-5 times, and mixing extracts to obtain an extract; (6) adding n-butanol into the extract to extract, standing at room temperature to layer to obtain the solution of n-butanol, extracting repeatedly for 4-7 times, mixing extracts to obtain the solution of n-butanol, and drying the solution of n-butanol to constant weight by reducing pressure to obtain the total triterpenes from the silverweed cinquefoil roots. The process provided by the invention is simple and efficient and is low in cost.
Description
Technical field
The present invention relates to a kind of extraction process of total triterpene, particularly relate to a kind of extraction process of Radix potentillae anserinae total triterpene.
Background technology
Radix potentillae anserinae formal name used at school is Potentilla anserina L., Rosaceae Potentilla, herbaceos perennial, and " Radix potentillae anserinae " refers generally to Potentilla anserina L. and expand tuber, one of comparatively conventional Tibetan medicine kind of China.Tuber has the effects such as cough-relieving of promoting the production of body fluid, invigorating spleen and reinforcing stomach, astringing to arrest bleeding, and the triterpenoid compound contained in tuber, is mainly triterpene acids and triterpene glycoside, has the pharmacologically actives such as antiinflammatory, antitumor, antibacterial, immunomodulating.Obtain application at field of medicaments at present.But, current total triterpene extraction process complex steps, and the dedoping step not relating to starch and sugar.Because in Radix potentillae anserinae, total triterpene contents is lower, and starch and sugared content very high, have a large amount of starch in leaching process and sugar is extracted, very large puzzlement caused to the total triterpene purification in later stage.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of extraction process of Radix potentillae anserinae total triterpene easy, efficient, with low cost.
For solving the problem, the extraction process of a kind of Radix potentillae anserinae total triterpene of the present invention, comprises the following steps:
(1) Radix potentillae anserinae root is after pulverizing, crossing 10 ~ 60 mesh sieves, obtains Radix potentillae anserinae root powder;
(2) be the alcoholic solution extraction of 50 ~ 95% by described Radix potentillae anserinae root powder mass concentration, obtain extracting solution, this extracting solution obtains filtrate after filtration, and described filtrate obtains extractum through concentrating under reduced pressure; The mass volume ratio of described Radix potentillae anserinae root powder and described alcoholic solution is 1g:10 ~ 30mL;
(3) add water sedimentation 2 ~ 3 times in described extractum, and left at room temperature 1 ~ 3h after stirring, then filters, obtain precipitum;
(4) the dehydrated alcohol adding its quality 2 ~ 5 times in described precipitum dissolves, obtain lysate, in this lysate, add the decolorization and impurity removal by active carbon of its volume 40% ~ 70%, reclaim ethanol, under temperature is 50 ~ 70 DEG C of conditions through drying under reduced pressure to constant weight, obtain crude extract;
(5) by the deionized water dissolving of described crude extract by its quality 2 ~ 10 times, then add ether after concussion extraction, left at room temperature layering, removes ether layer, repeats to merge afterwards for 3 ~ 5 times to be extracted liquid;
(6) in described extract, add the n-butyl alcohol of its volume 2 ~ 5 times, after concussion extraction, left at room temperature layering, get butanol solution part, re-extract 4 ~ 7 times, merge and obtain butanol solution, this butanol solution to constant weight, obtains Radix potentillae anserinae total-triterpene extract through drying under reduced pressure.
Described step (2) in extraction conditions refer to that temperature is 60 ~ 80 DEG C, extraction time 3 ~ 4 times, each 0.5 ~ 2.5 h.
Described step (2) in concentrating under reduced pressure condition refer to that temperature is 50 ~ 70 DEG C.
Described step is replaced (4) to prepare crude extract by following preparation method: the dehydrated alcohol adding its quality 2 ~ 5 times in described precipitum dissolves, obtain lysate, this lysate is adsorbed to the inspection of effluent maloic acid with the macroporous adsorptive resins AB-8 of its quality 2/3 times and is positive; Then use the washing post bed of lysate quality 5 ~ 6 times closely colourless to effluent, then use lysate quality 5 ~ 6 times and the ethanol elution that mass concentration is 95% is negative to ethanol elution, collect eluent, reclaim ethanol, obtain crude extract.
Described step (6) in drying under reduced pressure condition refer to that temperature is 60 ~ 80 DEG C.
The present invention compared with prior art has the following advantages:
1, because the present invention carries out sedimentation by after extract water dissolution, most starch and polysaccharide can be removed fast, therefore, for the purification in later stage reduces difficulty.
2, the present invention adopts macroporous resin to carry out purification process, just can obtain the higher Radix potentillae anserinae total triterpene of purity through water and ethanol two-step pretreatment.
3, the inventive method is simple to operate, and production cost is low, and gained Radix potentillae anserinae total triterpene composition has anticancer function, can be used for preparing the active drug that acceptable carrier can be made into multiple dosage form.
Detailed description of the invention
embodiment 1an extraction process for Radix potentillae anserinae total triterpene, comprises the following steps:
(1) 0.5kg Radix potentillae anserinae root is after pulverizing, crossing 10 mesh sieves, obtains Radix potentillae anserinae root powder.
(2) by Radix potentillae anserinae root powder mass concentration be 50% alcoholic solution be extract under the condition of 60 DEG C in temperature, extraction time 3 times, each 0.5 h, merges and obtains extracting solution, this extracting solution obtains filtrate after filtration, and filtrate obtains extractum through concentrating under reduced pressure under temperature is 50 DEG C of conditions.
Wherein: the mass volume ratio of Radix potentillae anserinae root powder and alcoholic solution is 1g:10mL.
(3) add water sedimentation 2 times in extractum, and left at room temperature 1 h after stirring, then filters, obtain precipitum.
(4) the dehydrated alcohol adding its quality 2 times in precipitum dissolves, and obtains lysate, adds the decolorization and impurity removal by active carbon of its volume 50% in this lysate, reclaim ethanol, under temperature is 50 DEG C of conditions through drying under reduced pressure to constant weight, obtain crude extract.
(5) by the deionized water dissolving of crude extract by its quality 2 times, then add ether after concussion extraction, left at room temperature layering, removes ether layer, repeats to merge afterwards for 4 times to be extracted liquid.
(6) in extract, add the n-butyl alcohol of its volume 2 times, after concussion extraction, left at room temperature layering, get butanol solution part, re-extract 4 times, merge obtain butanol solution, this butanol solution under temperature is 60 DEG C of conditions through drying under reduced pressure to constant weight, obtain Radix potentillae anserinae total-triterpene extract 9.6g.
embodiment 2an extraction process for Radix potentillae anserinae total triterpene, comprises the following steps:
(1) 1kg Radix potentillae anserinae root is after pulverizing, crossing 40 mesh sieves, obtains Radix potentillae anserinae root powder.
(2) by Radix potentillae anserinae root powder mass concentration be 60% alcoholic solution be extract under the condition of 70 DEG C in temperature, extraction time 4 times, each 1 h, merges and obtains extracting solution, this extracting solution obtains filtrate after filtration, and filtrate obtains extractum through concentrating under reduced pressure under temperature is 70 DEG C of conditions.
Wherein: the mass volume ratio of Radix potentillae anserinae root powder and alcoholic solution is 1g:20mL.
(3) add water sedimentation 3 times in extractum, and left at room temperature 2h after stirring, then filters, obtain precipitum.
(4) the dehydrated alcohol adding its quality 5 times in precipitum dissolves, and obtains lysate, adds the decolorization and impurity removal by active carbon of its volume 40% in this lysate, reclaim ethanol, under temperature is 70 DEG C of conditions through drying under reduced pressure to constant weight, obtain crude extract.
(5) by the deionized water dissolving of crude extract by its quality 5 times, then add ether after concussion extraction, left at room temperature layering, removes ether layer, repeats to merge afterwards for 3 times to be extracted liquid.
(6) in extract, add the n-butyl alcohol of its volume 5 times, after concussion extraction, left at room temperature layering, get butanol solution part, re-extract 5 times, merge obtain butanol solution, this butanol solution under temperature is 80 DEG C of conditions through drying under reduced pressure to constant weight, obtain Radix potentillae anserinae total-triterpene extract 13.5g.
embodiment 3an extraction process for Radix potentillae anserinae total triterpene, comprises the following steps:
(1) 10kg Radix potentillae anserinae root is after pulverizing, crossing 60 mesh sieves, obtains Radix potentillae anserinae root powder.
(2) by Radix potentillae anserinae root powder mass concentration be 95% alcoholic solution be extract under the condition of 80 DEG C in temperature, extraction time 3 times, each 2.5 h, merge and obtain extracting solution, this extracting solution obtains filtrate after filtration, and filtrate obtains extractum through concentrating under reduced pressure under temperature is 60 DEG C of conditions.
Wherein: the mass volume ratio of Radix potentillae anserinae root powder and alcoholic solution is 1g:30mL.
(3) add water sedimentation 2 times in extractum, and left at room temperature 3h after stirring, then filters, obtain precipitum.
(4) the dehydrated alcohol adding its quality 3 times in precipitum dissolves, and obtains lysate, adds the decolorization and impurity removal by active carbon of its volume 70% in this lysate, reclaim ethanol, under temperature is 60 DEG C of conditions through drying under reduced pressure to constant weight, obtain crude extract.
(5) by the deionized water dissolving of crude extract by its quality 10 times, then add ether after concussion extraction, left at room temperature layering, removes ether layer, repeats to merge afterwards for 5 times to be extracted liquid.
(6) in extract, add the n-butyl alcohol of its volume 3 times, after concussion extraction, left at room temperature layering, get butanol solution part, re-extract 7 times, merge obtain butanol solution, this butanol solution under temperature is 70 DEG C of conditions through drying under reduced pressure to constant weight, obtain Radix potentillae anserinae total-triterpene extract 120g.
embodiment 4an extraction process for Radix potentillae anserinae total triterpene, comprises the following steps:
(1) 0.5kg Radix potentillae anserinae root is after pulverizing, crossing 40 mesh sieves, obtains Radix potentillae anserinae root powder.
(2) by Radix potentillae anserinae root powder mass concentration be 70% alcoholic solution be extract under the condition of 70 DEG C in temperature, extraction time 3 times, each 1h, merge obtain extracting solution, this extracting solution obtains filtrate after filtration, and filtrate obtains extractum through concentrating under reduced pressure under temperature is 50 DEG C of conditions.
Wherein: the mass volume ratio of Radix potentillae anserinae root powder and alcoholic solution is 1g:20mL.
(3) add water sedimentation 3 times in extractum, and left at room temperature 1h after stirring, then filters, obtain precipitum.
(4) the dehydrated alcohol adding its quality 2 times in precipitum dissolves, and obtains lysate, and this lysate is adsorbed to the inspection of effluent maloic acid with the macroporous adsorptive resins AB-8 of its quality 2/3 times and is positive; Then use the washing post bed of lysate quality 5 times closely colourless to effluent, then by lysate quality 5 times and the ethanol elution that mass concentration is 95% be negative to ethanol elution, collect eluent, reclaim ethanol, obtain crude extract.
(5) by the deionized water dissolving of crude extract by its quality 2 times, then add ether after concussion extraction, left at room temperature layering, removes ether layer, repeats to merge afterwards for 3 times to be extracted liquid.
(6) in extract, add the n-butyl alcohol of its volume 2 times, after concussion extraction, left at room temperature layering, get butanol solution part, re-extract 5 times, merge obtain butanol solution, this butanol solution under temperature is 60 DEG C of conditions through drying under reduced pressure to constant weight, obtain Radix potentillae anserinae total-triterpene extract 10.8g.
embodiment 5an extraction process for Radix potentillae anserinae total triterpene, comprises the following steps:
(1) 1kg Radix potentillae anserinae root is after pulverizing, crossing 30 mesh sieves, obtains Radix potentillae anserinae root powder.
(2) by Radix potentillae anserinae root powder mass concentration be 80% alcoholic solution be extract under the condition of 80 DEG C in temperature, extraction time 3 times, each 2 h, merge and obtain extracting solution, this extracting solution obtains filtrate after filtration, and filtrate obtains extractum through concentrating under reduced pressure under temperature is 60 DEG C of conditions.
Wherein: the mass volume ratio of Radix potentillae anserinae root powder and alcoholic solution is 1g:30mL.
(3) add water sedimentation 3 times in extractum, and left at room temperature 3h after stirring, then filters, obtain precipitum.
(4) the dehydrated alcohol adding its quality 5 times in precipitum dissolves, and obtains lysate, and this lysate is adsorbed to the inspection of effluent maloic acid with the macroporous adsorptive resins AB-8 of its quality 2/3 times and is positive; Then use the washing post bed of lysate quality 6 times closely colourless to effluent, then by lysate quality 6 times and the ethanol elution that mass concentration is 95% be negative to ethanol elution, collect eluent, reclaim ethanol, obtain crude extract.
(5) by the deionized water dissolving of crude extract by its quality 6 times, then add ether after concussion extraction, left at room temperature layering, removes ether layer, repeats to merge afterwards for 5 times to be extracted liquid.
(6) in extract, add the n-butyl alcohol of its volume 5 times, after concussion extraction, left at room temperature layering, get butanol solution part, re-extract 7 times, merge obtain butanol solution, this butanol solution under temperature is 80 DEG C of conditions through drying under reduced pressure to constant weight, obtain Radix potentillae anserinae total-triterpene extract 14.7g.
embodiment 6an extraction process for Radix potentillae anserinae total triterpene, comprises the following steps:
(1) 10kg Radix potentillae anserinae root is after pulverizing, crossing 60 mesh sieves, obtains Radix potentillae anserinae root powder.
(2) by Radix potentillae anserinae root powder mass concentration be 95% alcoholic solution be extract under the condition of 80 DEG C in temperature, extraction time 4 times, each 2 h, merge and obtain extracting solution, this extracting solution obtains filtrate after filtration, and filtrate obtains extractum through concentrating under reduced pressure under temperature is 70 DEG C of conditions.
Wherein: the mass volume ratio of Radix potentillae anserinae root powder and alcoholic solution is 1g:30mL.
(3) add water sedimentation 3 times in extractum, and left at room temperature 2h after stirring, then filters, obtain precipitum.
(4) the dehydrated alcohol adding its quality 4 times in precipitum dissolves, and obtains lysate, and this lysate is adsorbed to the inspection of effluent maloic acid with the macroporous adsorptive resins AB-8 of its quality 2/3 times and is positive; Then use the washing post bed of lysate quality 5.5 times closely colourless to effluent, then by lysate quality 5.5 times and the ethanol elution that mass concentration is 95% be negative to ethanol elution, collect eluent, reclaim ethanol, obtain crude extract.
(5) by the deionized water dissolving of crude extract by its quality 8 times, then add ether after concussion extraction, left at room temperature layering, removes ether layer, repeats to merge afterwards for 5 times to be extracted liquid.
(6) in extract, add the n-butyl alcohol of its volume 4 times, after concussion extraction, left at room temperature layering, get butanol solution part, re-extract 7 times, merge obtain butanol solution, this butanol solution under temperature is 70 DEG C of conditions through drying under reduced pressure to constant weight, obtain Radix potentillae anserinae total-triterpene extract 126g.
Claims (5)
1. an extraction process for Radix potentillae anserinae total triterpene, comprises the following steps:
(1) Radix potentillae anserinae root is after pulverizing, crossing 10 ~ 60 mesh sieves, obtains Radix potentillae anserinae root powder;
(2) be the alcoholic solution extraction of 50 ~ 95% by described Radix potentillae anserinae root powder mass concentration, obtain extracting solution, this extracting solution obtains filtrate after filtration, and described filtrate obtains extractum through concentrating under reduced pressure; The mass volume ratio of described Radix potentillae anserinae root powder and described alcoholic solution is 1g:10 ~ 30mL;
(3) add water sedimentation 2 ~ 3 times in described extractum, and left at room temperature 1 ~ 3h after stirring, then filters, obtain precipitum;
(4) the dehydrated alcohol adding its quality 2 ~ 5 times in described precipitum dissolves, obtain lysate, in this lysate, add the decolorization and impurity removal by active carbon of its volume 40% ~ 70%, reclaim ethanol, under temperature is 50 ~ 70 DEG C of conditions through drying under reduced pressure to constant weight, obtain crude extract;
(5) by the deionized water dissolving of described crude extract by its quality 2 ~ 10 times, then add ether after concussion extraction, left at room temperature layering, removes ether layer, repeats to merge afterwards for 3 ~ 5 times to be extracted liquid;
(6) in described extract, add the n-butyl alcohol of its volume 2 ~ 5 times, after concussion extraction, left at room temperature layering, get butanol solution part, re-extract 4 ~ 7 times, merge and obtain butanol solution, this butanol solution to constant weight, obtains Radix potentillae anserinae total-triterpene extract through drying under reduced pressure.
2. the extraction process of a kind of Radix potentillae anserinae total triterpene as claimed in claim 1, is characterized in that: described step (2) in extraction conditions refer to that temperature is 60 ~ 80 DEG C, extraction time 3 ~ 4 times, each 0.5 ~ 2.5 h.
3. the extraction process of a kind of Radix potentillae anserinae total triterpene as claimed in claim 1, is characterized in that: described step (2) in concentrating under reduced pressure condition refer to that temperature is 50 ~ 70 DEG C.
4. the extraction process of a kind of Radix potentillae anserinae total triterpene as claimed in claim 1, it is characterized in that: replace step described in claim 1 (4) to prepare crude extract by following preparation method: the dehydrated alcohol adding its quality 2 ~ 5 times in described precipitum dissolves, obtain lysate, this lysate is adsorbed to the inspection of effluent maloic acid with the macroporous adsorptive resins AB-8 of its quality 2/3 times and is positive; Then use the washing post bed of lysate quality 5 ~ 6 times closely colourless to effluent, then use lysate quality 5 ~ 6 times and the ethanol elution that mass concentration is 95% is negative to ethanol elution, collect eluent, reclaim ethanol, obtain crude extract.
5. the extraction process of a kind of Radix potentillae anserinae total triterpene as claimed in claim 1, is characterized in that: described step (6) in drying under reduced pressure condition refer to that temperature is 60 ~ 80 DEG C.
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| CN201510015721.4A CN104622999B (en) | 2015-01-13 | 2015-01-13 | A kind of extraction process of fern fiber crops total triterpene |
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| CN201510015721.4A CN104622999B (en) | 2015-01-13 | 2015-01-13 | A kind of extraction process of fern fiber crops total triterpene |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104958393A (en) * | 2015-06-08 | 2015-10-07 | 中国科学院西北高原生物研究所湖州高原生物资源产业化创新中心 | Extract of potentilla anserina rhizome and medical application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1374079A (en) * | 2002-03-26 | 2002-10-16 | 中国人民解放军第三○二医院 | Antiviral drug containing silverweed cinquefoil essence and its prepn |
-
2015
- 2015-01-13 CN CN201510015721.4A patent/CN104622999B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1374079A (en) * | 2002-03-26 | 2002-10-16 | 中国人民解放军第三○二医院 | Antiviral drug containing silverweed cinquefoil essence and its prepn |
Non-Patent Citations (2)
| Title |
|---|
| 王舒: "蕨麻正丁醇层化学成分及抗缺氧活性研究", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
| 褚良等，: "藏药蕨麻的化学成分研究", 《中国现代中药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104958393A (en) * | 2015-06-08 | 2015-10-07 | 中国科学院西北高原生物研究所湖州高原生物资源产业化创新中心 | Extract of potentilla anserina rhizome and medical application thereof |
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| CN104622999B (en) | 2017-06-30 |
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