CN104606263B - A kind of extract and its quality determining method of purslane - Google Patents
A kind of extract and its quality determining method of purslane Download PDFInfo
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- CN104606263B CN104606263B CN201410830970.4A CN201410830970A CN104606263B CN 104606263 B CN104606263 B CN 104606263B CN 201410830970 A CN201410830970 A CN 201410830970A CN 104606263 B CN104606263 B CN 104606263B
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Abstract
The invention discloses a kind of extracts of purslane, it is prepared by following technique: taking purslane powder, are extracted with water, filter, obtain filtrate, be evaporated.The invention also discloses the quality determining methods of purslane, it is the following steps are included: a, test solution preparation；B, prepared by reference substance solution: threonine and leucine reference substance, and reference substance solution is made in dissolution；C, detect: aforementioned test solution and control solution, point using n-butanol-acetic acid-alcohol-water as solvent, are unfolded on silica gel g thin-layer plate, take out, dry, spray with 0.2% ethanol solution of ninhydrin, it is clear to be heated to spot development at 110 DEG C.Extract of the present invention can be used as the standard items of control purslane quality, have extremely strong practical application value；The quality determining method of purslane of the present invention can by simultaneously detect threonine and leucine, determine the quality of purslane, it is easy to operate, quick, have a good application prospect.
Description
Technical field
The present invention relates to a kind of extract of purslane and its quality determining methods.
Background technique
The portulacaceous annual meat herbaceous plant of Portulaca also known as long life dish, Wu Hangcao, melon seeds dish etc., except height
Cold area is outer to be distributed all over the world.The purslane resource very abundant in China has long go through to the utilization of purslane
History, in Northern and Southern Dynasties' period to its function with regard on the books.In the existing national statutory standards in China, purslane is recorded in " China
Veterinary drug allusion quotation " two, have it is clearing heat and detoxicating, the effect of removing heat from the blood and relieve diarrhea.Modern pharmacological studies have shown that purslane has anti-aging, treatment
Diabetes, prevention coronary heart disease, preventing hypertension, prevention obesity, inhibit growth of tumour cell and improve immunity of organism reducing blood lipid
The function of ability.
Currently, the identification of purslane is with control medicinal material according to the record of " Pharmacopoeia of People's Republic of China (2010 editions) "
It for reference substance, is detected using thin-layer chromatography, the preparation method of test solution is to add 10 times of weight in purslane powder
Water adds first acid for adjusting pH value to 3-4, cold soaking 3h, and filtration, filtrate is evaporated, and residue adds water 1ml to make to dissolve, the stationary phase used for
Silica gel g thin-layer plate；Solvent is water-saturated n-butanol-glacial acetic acid-water, volume 4:1:1.In sample chromatogram, with compare
On the corresponding position of medicinal material chromatography, the spot of same color is shown.In thin-layer chromatography made from this kind of method, spot is more, to behaviour
Make the more demanding of personnel.
Summary of the invention
The present invention provides a kind of extract of purslane and its quality determining methods.
The extract of purslane of the present invention, it is prepared by following technique: purslane powder taken, is extracted with water, is filtered,
Filtrate is obtained, is evaporated.
Preferably, the filtrate the preparation method comprises the following steps: take purslane powder, add the water of 20 times of weight, formic acid be added and adjusts
PH value is filtered to 3-4, ultrasonic extraction 30min.
The present invention provides a kind of quality determining method of purslane, it the following steps are included:
A, aforementioned extract is taken, dissolves, test solution is made；
B, prepared by reference substance solution: threonine and leucine reference substance, and reference substance solution is made in dissolution；
C, detect: aforementioned test solution and control solution, point is on silica gel g thin-layer plate, with n-butanol-acetic acid-second
Alcohol-water is solvent, is unfolded, and takes out, dries, and sprays with 0.2% ethanol solution of ninhydrin, it is clear to be heated to spot development at 110 DEG C
It is clear.
Wherein, reference substance described in step b adds methanol that Soviet Union's ammonia is made the preparation method comprises the following steps: take threonine, leucine standard
Acid, leucine concentration are respectively the reference substance solution of 1mg/ml.
Wherein, solvent described in step c is n-butanol-acetic acid-alcohol-water, volume ratio 4:1:1:1.
The present invention also provides a kind of discrimination methods of purslane true and false, it includes the following steps:
(1) measuring samples are taken；
(2) it is detected by detection method above-mentioned；
(3) Analysis of test results: measuring samples and reference substance spot having the same, as purslane certified products.
Extract of the present invention can be used as the standard items of control purslane quality, have extremely strong practical application value；This
The quality determining method of invention purslane can determine the quality of purslane, operate by detecting threonine and leucine simultaneously
It is easy, quick, it has a good application prospect.
The present invention is described in further details below by specific embodiment, but is not to limit of the invention
System, above content according to the present invention, according to the ordinary technical knowledge and customary means of this field, not departing from, the present invention is above-mentioned
Under the premise of basic fundamental thought, the modification, replacement or change of other diversified forms can also be made.
Detailed description of the invention
Fig. 1 testing result figure of the present invention, 1: threonine, 2: leucine, 3: purslane medicinal material.
Specific embodiment
The preparation method of the extract of the present invention of embodiment 1
Purslane is dried, powder is broken into, takes powder 2g, adds water 20ml, adds first acid for adjusting pH value to 3-4, ultrasonic 30min,
Filtration, filtrate water bath method to get.
The quality determining method of the invention of embodiment 2
Purslane is dried, powder is broken into, takes powder 2g, adds water 20ml, adds first acid for adjusting pH value to 3-4, ultrasonic 30min,
Filtration, filtrate water bath method, residue adds water 1ml to make to dissolve, as test solution.Threonine and leucine reference substance separately are taken,
Add methanol that solution of every 1ml containing 1mg is made, as reference substance solution.It is tested according to thin-layered chromatography, it is each to draw above-mentioned three kinds of solution
2 μ l are put respectively on same silica gel g thin-layer plate, and with n-butanol-acetic acid-alcohol-water (4:1:1:1) for solvent, expansion is taken
Out, it dries, sprays with 0.2% ethanol solution of ninhydrin, it is clear to be heated to spot development at 110 DEG C.
If as shown in Figure 1, at the position corresponding to the chromatogram of the reference substance, showing the glimmering of same color in sample chromatogram
Hot spot point illustrates that the quality of purslane test sample is good；If in sample chromatogram, in position corresponding with reference substance chromatography
On, the fluorescence spot of same color, then do not illustrate that the quality of purslane test sample is bad.
To sum up, extract of the present invention can be used as the standard items of control purslane quality, have extremely strong practical application valence
Value；The quality determining method of purslane of the present invention can by simultaneously detect threonine and leucine, determine the quality of purslane,
It is easy to operate, quick.
Claims (4)
1. a kind of quality determining method of purslane, it is characterised in that: it the following steps are included:
A, purslane extract is taken, dissolves, test solution is made；The purslane extract is prepared by following technique:
Purslane powder is taken, is extracted with water, is filtered, is obtained filtrate, be evaporated；Wherein, the filtrate the preparation method comprises the following steps: taking dent
Amaranth powder adds the water of 20 times of weight, and first acid for adjusting pH value is added to 3-4, ultrasonic extraction 30min, filtration；
B, prepared by reference substance solution: threonine and leucine reference substance, and reference substance solution is made in dissolution；
C, detect: aforementioned test solution and control solution, point is on silica gel g thin-layer plate, with n-butanol-acetic acid-alcohol-water
For solvent, it is unfolded, takes out, dry, sprays with 0.2% ethanol solution of ninhydrin, it is clear to be heated to spot development at 110 DEG C.
2. quality determining method according to claim 1, it is characterised in that: reference substance described in step b the preparation method comprises the following steps:
Take threonine, leucine standard, add methanol that threonine is made, leucine concentration be respectively 1mg/ml reference substance solution.
3. quality determining method according to claim 1, it is characterised in that: solvent described in step c is n-butanol-second
Acid-alcohol-water, volume ratio 4:1:1:1.
4. a kind of discrimination method of the purslane true and false, it includes the following steps:
(1) measuring samples are taken；
(2) by the detection of detection method described in claim 1-3 any one；
(3) Analysis of test results: measuring samples and reference substance spot having the same, as purslane certified products.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410830970.4A CN104606263B (en) | 2014-12-26 | 2014-12-26 | A kind of extract and its quality determining method of purslane |
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| CN201410830970.4A CN104606263B (en) | 2014-12-26 | 2014-12-26 | A kind of extract and its quality determining method of purslane |
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| CN104606263A CN104606263A (en) | 2015-05-13 |
| CN104606263B true CN104606263B (en) | 2019-09-10 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105028491B (en) * | 2015-08-07 | 2017-09-29 | 上海海洋大学 | A kind of duckweed biological source growth promoter and its preparation method and application |
| CN106526058A (en) * | 2016-11-03 | 2017-03-22 | 成都乾坤动物药业股份有限公司 | Thin-layer chromatography-fluorescence analysis method for heat-clearing dysentery-stopping granules |
| CN106727762A (en) * | 2016-12-29 | 2017-05-31 | 广州凯耀资产管理有限公司 | The natural component extracting method of purslane |
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Non-Patent Citations (1)
| Title |
|---|
| 马齿苋水提取物对小鼠急性炎症的影响;范红艳等;《延边大学医学学报》;20060930;第29卷(第3期);第184-185页 |
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Address after: No. 259, Middle Section of Jinfu Road, Wenjiang District, Chengdu City, Sichuan Province, 611130 Patentee after: CHENGDU QIANKUN VETERINARY PHARMACEUTICAL CO.,LTD. Address before: 611130 no.259 Jinfu Road, Chengdu cross strait science and Technology Industrial Development Park, Wenjiang District, Chengdu City, Sichuan Province Patentee before: CHENGDU QIANKUN VETERINARY PHARMACEUTICAL Co.,Ltd. |