CN104597294A - Anchor for fixing tissue slice in in-vitro electrophysiological experiment - Google Patents
Anchor for fixing tissue slice in in-vitro electrophysiological experiment Download PDFInfo
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- CN104597294A CN104597294A CN201410781476.3A CN201410781476A CN104597294A CN 104597294 A CN104597294 A CN 104597294A CN 201410781476 A CN201410781476 A CN 201410781476A CN 104597294 A CN104597294 A CN 104597294A
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Abstract
The invention belongs to the technical field of apparatuses and instruments for medical treatment experiment, specifically relates to an anchor for fixing a tissue slice in an in-vitro electrophysiological experiment, and aims at solving the technical problems of the existing anchor for fixing the tissue slice in the in-vitro electrophysiological experiment that the brain tissue cannot be closely contacted with an electrode, the brain tissue cannot be prevented from being flushed away by circularly supplied nutrient perfusate, and the experiment record is greatly interfered. According to the technical scheme, the anchor for fixing the tissue slice in the in-vitro electrophysiological experiment comprises an anchor frame formed by integrating two arc sections and two clamping lugs, and a fixing screen which is arranged between the two arc sections of the anchor frame. The anchor has the advantages of enabling close contact of the tissue slice and the electrode, preventing the tissue slice from being flushed away by circularly supplied nutrient perfusate, and avoiding the interference to the experiment record.
Description
Technical field
The invention belongs to medical experiment technique with the apparatus field, be specifically related to a kind of anchor of in vitro electro physiology experiment fixed tissue slices.
Background technology
MED64 planar microelectrode array register system is the outer Electrophysiology research tool of the in vitro born of the same parents of hyperchannel produced by Japanese Alpha Med Science company.System includes full set of hardware equipment consumptive material needed in vitro electrophysiologic study and analysis software.User can quickly and easily to To body material as the materials such as the neurocyte of brain sheet, cultivation carry out electro physiology detection; The local electric change of extracellular direct neighbor can be measured, simultaneously and the electricity physiological signal of the multiple cell of line item.MED64 equipment can record simultaneously and stimulate different loci; And MED64 record or non-intrusion type, therefore can not damaging cells, this characteristic makes it be applicable on same sample, carry out long time-histories record and repeat record.When MED64 records, need on brain sheet, add a cover an anchor, to contact with electrode seal to help brain tissue and the perfusate of avoiding brain tissue to be recycled nutrition washed away.But anchor used at present exists following technical matters: on the one hand, because its quality kicks the beam, brain tissue can not be effectively made to contact with electrode seal and the perfusate that brain tissue can not be avoided to be recycled nutrition washed away; On the other hand because its shape and size improper, above the electrode being pressed in MED64, huge interference can be caused to experimental record.
Summary of the invention
The perfusate that what the anchor that the object of the invention is to solve existing in vitro electro physiology experiment fixed tissue slices existed can not contact brain tissue with electrode seal effectively, brain tissue can not be avoided to be recycled supply nutrition washes away and experimental record is caused to the technical matters of huge interference, provides a kind of anchor of in vitro electro physiology experiment fixed tissue slices.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
An anchor in vitro electro physiology experiment fixed tissue slices, it is made up of two sections of circular arcs and the one-body molded anchor rack that forms of two ear and fixed network, and described fixed network is located between two circular arcs of anchor rack.
Described anchor rack is greater than 7.9g/cm by density
3nontoxic stainless hard metal material make.
Described fixed network is nylon wire.
The present invention adopts above technical scheme, and the anchor of in vitro electro physiology experiment fixed tissue slices is made up of two sections of circular arcs and the one-body molded anchor rack that forms of two ear and fixed network, and anchor rack adopts density to be greater than 7.9g/cm
3nontoxic stainless hard metal material make, the anchor being provided with fixed network circular shape is added a cover on tissue sections, histotomy is contacted with electrode seal and the perfusate of avoiding histotomy to be recycled nutrition washes away, and interference can not be caused to experimental record.Therefore, the present invention have can effectively make histotomy contact with electrode seal, can avoid histotomy be recycled the perfusate of nutrition wash away and can not cause experimental record the advantage of interference.
For showing that the present invention has above advantage, carried out spontaneous discharge local field potentials (LFP) and excitatory postsynaptic potential (EPSP) (fEPSP) record of mouse hippocampal slices, its result is shown in Fig. 3 Fig. 4 and Fig. 5 Fig. 6 respectively.
In spontaneous discharge local field potentials (LFP) record, the property that its raw readings figure (Fig. 3) discharges is made an uproar higher, and it can analyze the frequency distribution of brain sheet spontaneous discharge effectively as seen to analyze (Fig. 4) to raw readings figure.
In excitatory postsynaptic potential (EPSP) (fEPSP) record, visible excitatory postsynaptic potential (EPSP) (fEPSP) waveform of its raw readings (Fig. 5) is regular; Stablize basic ripple before the visible high frequency stimulation of this raw readings map analysis (Fig. 6), after high frequency stimulation, the amplitude of excitatory postsynaptic potential (EPSP) (fEPSP) obviously increases, and maintains record good after high frequency stimulation.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is using state figure of the present invention;
Fig. 3 is the raw readings figure of the spontaneous discharge local field potentials (LFP) be recorded to the present invention;
Fig. 4 is spontaneous discharge local field potentials (LFP) frequency plot remembered with the present invention;
Fig. 5 is the original waveform figure of the excitatory postsynaptic potential (EPSP) (fEPSP) remembered with the present invention;
Fig. 6 is the scatter diagram of excitatory postsynaptic potential (EPSP) (fEPSP) amplitude remembered with the present invention.
Embodiment
As shown in Figure 1, the anchor of a kind of in vitro electro physiology experiment fixed tissue slices in the present embodiment, it is made up of two sections of circular arcs 1 and the one-body molded anchor rack that forms of two ear 2 and fixed network 3, and described fixed network 3 is located between two circular arcs 1 of anchor rack.
Described anchor rack is greater than 7.9g/cm by density
3nontoxic stainless hard metal material make.
Described fixed network 3 is nylon wire.
The present invention is the course of work: the mouse brain slices of first hatching is positioned on MED64 planar microelectrode, and cover the anchor of fixed tissue slices in the experiment of in vitro electro physiology lightly, the position of adjustment brain sheet and anchor, carries out experimental record.
Claims (3)
1. the anchor of an in vitro electro physiology experiment fixed tissue slices, it is characterized in that: it is made up of the anchor rack of two sections of circular arcs (1) and two one-body molded formations of ear (2) and fixed network (3), and described fixed network (3) is located between two circular arcs (1) of anchor rack.
2. the anchor of a kind of in vitro electro physiology experiment fixed tissue slices according to claim 1, is characterized in that: described anchor rack is greater than 7.9g/cm by density
3nontoxic stainless hard metal material make.
3. the anchor of a kind of in vitro electro physiology experiment fixed tissue slices according to claim 1, is characterized in that: described fixed network (3) is nylon wire.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410781476.3A CN104597294A (en) | 2014-12-16 | 2014-12-16 | Anchor for fixing tissue slice in in-vitro electrophysiological experiment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410781476.3A CN104597294A (en) | 2014-12-16 | 2014-12-16 | Anchor for fixing tissue slice in in-vitro electrophysiological experiment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104597294A true CN104597294A (en) | 2015-05-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201410781476.3A Pending CN104597294A (en) | 2014-12-16 | 2014-12-16 | Anchor for fixing tissue slice in in-vitro electrophysiological experiment |
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Citations (4)
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|---|---|---|---|---|
| US5380337A (en) * | 1994-01-24 | 1995-01-10 | Romaine; Richard A. | Mesh-type skin biopsy appliance |
| CN103091826A (en) * | 2012-12-21 | 2013-05-08 | 中国人民解放军第三军医大学第三附属医院 | Set of glass slides used for carrying out immunohistochemistry staining and storage to tissue slice |
| CN103826553A (en) * | 2011-07-22 | 2014-05-28 | R·萨利赫 | Surgical retrieval apparatus and method with semi-rigidly extendable and collapsable basket |
| CN204374237U (en) * | 2014-12-16 | 2015-06-03 | 山西医科大学 | A kind of anchor of in vitro electro physiology experiment fixed tissue slices |
-
2014
- 2014-12-16 CN CN201410781476.3A patent/CN104597294A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5380337A (en) * | 1994-01-24 | 1995-01-10 | Romaine; Richard A. | Mesh-type skin biopsy appliance |
| CN103826553A (en) * | 2011-07-22 | 2014-05-28 | R·萨利赫 | Surgical retrieval apparatus and method with semi-rigidly extendable and collapsable basket |
| CN103091826A (en) * | 2012-12-21 | 2013-05-08 | 中国人民解放军第三军医大学第三附属医院 | Set of glass slides used for carrying out immunohistochemistry staining and storage to tissue slice |
| CN204374237U (en) * | 2014-12-16 | 2015-06-03 | 山西医科大学 | A kind of anchor of in vitro electro physiology experiment fixed tissue slices |
Non-Patent Citations (3)
| Title |
|---|
| YUN-FEI LU等: "Spatial and temporal plasticity of synaptic organization in anterior cingulate cortex following peripheral infl ammatory pain:multi-electrode array recordings in rats", 《NEUROSCI BULL》 * |
| 许燕等: "5-HT2C受体亚型参与易化大鼠内嗅区-海马通路的突触传递：平面微电极阵列记录技术研究", 《生理学报》 * |
| 黄宏平: "蓝斑神经元去甲肾上腺素量子化释放的长潜伏期：大鼠脑片膜片钳-电化学联合记录研究", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
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Application publication date: 20150506 |