CN104592413A - Pectin production technology - Google Patents

Abstract

The invention discloses a pectin production technology, which comprises the steps of: taking pericarp and pomace to conduct high temperature rinsing for 30min, controlling the temperature at 85-90DEG C; filtering the solid-liquid by solid-liquid separation equipment, collecting the filtrate, then filtering the collected filtrate to obtain a clarified and transparent solution without obvious solid and precipitate; adding acid to the finally obtained clarified and transparent solution to adjust the pH value to 2, and carrying out reaction for 10min; subjecting the acidic solution to membrane concentration, firstly using membrane I with a molecular weight cut-off of 50000, then using membrane II to filter out small molecules less than 500 to obtain a solution with molecules of 500-50000, and carrying out alcohol precipitation, drying, crushing and sieving to obtain the product. The technology provided by the invention overcomes the defects of the prior art, the design process is simple and reasonable, pectin with a molecular weight of less than 50000 can be obtained, and the reaction yield is very good.

Description

A kind of production technique of pectin

Technical field

The present invention relates to pectin production technical field, specifically belong to a kind of production technique of pectin.

Background technology

Pectin is the natural macromolecular polysaccharide polymkeric substance of a class, and it represents one group of polysaccharide of close association between higher plant primary wall and flanking cell, also represents the complicated gluey polymer of a group prepared from vegetable material.Its basic structure is in units of galacturonic acid.Its main component is a (Isosorbide-5-Nitrae) D mono-polygalacturonic acid of part esterification.Residual carboxyl units exists with the form of free acid or forms the salt such as ammonium, potassium sodium and calcium.

Pectin has the effects such as antibacterial, hemostasis, detumescence, removing toxic substances, antidiarrheal, reducing blood-fat, radioprotective, is a kind of excellent pharmaceutical preparation matrix, and has nontoxic and bio-compatibility due to pectin, or as the excellent material of pharmaceutical carrier.Wherein pectin as carrier in vivo renal excretion molecular weight should control below 50,000.

Current small molecules pectin obtains mainly through chemical method and enzymic degradation natural pectin, and dilute acid hydrolysis pectin is normally used in chemical method degraded, and maximum feature is that degradation speed is fast, obtains a large amount of monose, is difficult to obtain oligose.Put down and owing to introducing various reaction reagent in degradation process, make to increase the control difficulty of its DeR process, also make the separation and purification work of degraded product not easily go on.Therefore, the simple acid-hydrolyzed method of current less employing prepares small molecules pectin.Enzymic degradation pectin polysaccharide, namely selects one or more enzymes specific to degrade to pectin molecule, allows it optionally cut off a (Isosorbide-5-Nitrae) D mono-polygalacturonic acid in pectin molecule, thus obtains small molecules pectin.But the method usually only can obtain the pectin that monose or disaccharides are difficult to obtain molecular weight less than 50,000.

Summary of the invention

The object of this invention is to provide a kind of production technique of pectin, overcome the deficiencies in the prior art, design technology advantages of simple, can obtain the pectin that molecular weight is less than 50,000, reaction yield is fine.

The technical solution used in the present invention is as follows:

A production technique for pectin, this described production craft step is as follows, the first step: get pericarp, pomace carries out high-temperature rinsing 30min, temperature controls at 85-90 DEG C;

Second step: filtered by solid-liquid separating equipment by above-mentioned solid-liquid, is collected filtrate, the filtrate of collection is filtered again, obtains clear, without obvious solid, without the solution precipitated;

3rd step: second step finally being obtained clear transparent solutions, to add acid-conditioning solution ph value be 2, reaction 10min;

4th: by the acidic solution of the 3rd step, carry out membrane concentration, first use film I, molecular weight cut-off 50000, then use film II, filter out the small molecules being less than 500, to the solution of molecule between 500-50000 be obtained, carry out that alcohol is analysed, dries, pulverized, sieving obtains product.

Described acid is formic acid, acetic acid, hydrochloric acid or sulfuric acid.

Described concentration of hydrochloric acid is 34%-36%.

Compared with the prior art, beneficial effect of the present invention is as follows:

Present invention process flow scheme design is reasonable, does not need special equipment, and reaction yield is better, obtains pectin and is molecular weight and is less than less than 50,000, have higher medical value.

Embodiment

A production technique for pectin, this described production craft step is as follows, the first step: get pericarp, pomace carries out high-temperature rinsing 30min, temperature controls at 85-90 DEG C;

Second step: filtered by solid-liquid separating equipment by above-mentioned solid-liquid, is collected filtrate, the filtrate of collection is filtered again, obtains clear, without obvious solid, without the solution precipitated;

3rd step: second step finally being obtained clear transparent solutions, to add acid-conditioning solution ph value be 2, reaction 10min;

4th: by the acidic solution of the 3rd step, carry out membrane concentration, first use film I, molecular weight cut-off 50000, then use film II, filter out the small molecules being less than 500, to the solution of molecule between 500-50000 be obtained, carry out that alcohol is analysed, dries, pulverized, sieving obtains product.

Embodiment is above only be described the preferred embodiment of the present invention; not scope of the present invention is limited; do not departing under the present invention designs spiritual prerequisite; the various distortion that the common engineering technical personnel in this area make technical solution of the present invention and improvement, all should fall in protection domain that claims of the present invention determine.

Claims (1)

1. a production technique for pectin, is characterized in that:

This described production craft step is as follows,

The first step: get pericarp, pomace carries out high-temperature rinsing 30min, temperature controls at 85-90 DEG C;

Second step: filtered by solid-liquid separating equipment by above-mentioned solid-liquid, is collected filtrate, the filtrate of collection is filtered again, obtains clear, without obvious solid, without the solution precipitated;

3rd step: second step finally being obtained clear transparent solutions, to add acid-conditioning solution ph value be 2, reaction 10min;

4th: by the acidic solution of the 3rd step, carry out membrane concentration, first use film I, molecular weight cut-off 50000, then use film II, filter out the small molecules being less than 500, to the solution of molecule between 500-50000 be obtained, carry out that alcohol is analysed, dries, pulverized, sieving obtains product.