CN104585681A - Preparation method of pickle containing cordyceps sinensis - Google Patents

Preparation method of pickle containing cordyceps sinensis Download PDF

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Publication number
CN104585681A
CN104585681A CN201510011862.9A CN201510011862A CN104585681A CN 104585681 A CN104585681 A CN 104585681A CN 201510011862 A CN201510011862 A CN 201510011862A CN 104585681 A CN104585681 A CN 104585681A
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China
Prior art keywords
caterpillar fungus
chinese caterpillar
add
pickle
fermentation
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CN201510011862.9A
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Chinese (zh)
Inventor
李政
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TIANJIN TIANLVJIAN TECHNOLOGY Co Ltd
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TIANJIN TIANLVJIAN TECHNOLOGY Co Ltd
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Priority to CN201510011862.9A priority Critical patent/CN104585681A/en
Publication of CN104585681A publication Critical patent/CN104585681A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/169Plantarum

Abstract

The invention discloses a preparation method of pickle containing cordyceps sinensis. The method comprises the following steps: weighing white radishes, fresh gingers and garlic, evenly mixing and putting the mixture into a pickle jar; feeding mixed fungus powder into a vegetable raw material, wherein the mass of the mixed fungus powder accounts for 0.05-0.2% of that of the vegetable raw material; covering the pickle jar by a cover, sealing by water, and guaranteeing the oxygen-free environment in the jar; carrying out fermentation at the temperature of 20-30 DEG C for 5-8 hours, then feeding cordyceps sinensis enzymatic hydrolysate, and continuously fermenting for 58-80 hours, wherein the mixed fungus powder comprises the following components in parts by weight: lactobacillus plantarum and acetobacter aceti. The product is richer in fragrance and moderate in crispness, and the comprehensive sensory evaluation of the pickle containing the cordyceps sinensis is higher than that of commercially available products.

Description

A kind of pickle production method of adding Chinese caterpillar fungus
Technical field:
The present invention utilizes Cordyceps sinensis to reduce Nitrite in Pickles content and improves the method for pickle flavor, belongs to field of food.
Technical background:
Pickles are a kind of traditional foods of China, have the long history of more than 2000 year.It is mixed fermentation vegetables based on lactic acid bacteria in natural environment and flavoring, because local flavor is good to eat, be easy to store, nutrient health, therefore become a kind of popular food that Chinese generally like.
But the nitrite problem in pickles always is and receives much concern.Nitrite in pickles due in pickled and sweat nitrate reductase be that nitrite causes by the nitrate reduction in vegetables.After human body takes in nitrite, nitrogen-containing compound (secondary amine, tertiary amine, acid amides and amino acid) in nitrous acid and stomach is combined into the health of the direct harmful to human of the nitrosamine with carcinogenicity, people are quite scrupled for the consumption of pickles, and this becomes one of key factor of restriction pickles development.
Clear stipulaties in national standard (GB2762 ?2005), the content of catsup and pickled vegetables nitrite should more than 20mg/kg sample.In order to reduce the content of nitrite in catsup and pickled vegetables, Chinese scholars has carried out in many ways making great efforts.
At present, the method reducing Nitrite in Pickles mainly contains two classes: a class is by biodegradable method, namely the fermentation strain of the artificial method seed selection high degradability be separated is adopted, carry out method that is purebred or fermented by mixed bacterium, get rid of reproducibility as far as possible and to mix the increase of microbial content of nitrite.Another kind of is adopt the method for physics and chemistry, by the negative effect regulating the osmotic pressure of zymotic fluid or regulate the one-tenth of zymotic fluid to assign to suppress reproducibility microorganism to cause, as added material containing sulfydryl, hydroxyl to reduce the content of nitrite.
And under study for action, the discovery of our novelty adds cordyceps sporophore enzymolysis liquid can reduce content of nitrite in pickles, and successful.The present invention, by adding cordyceps sporophore enzymolysis liquid in the curing process of pickles, provides a kind of new technology and thinking for reducing Nitrite in Pickles content.
Summary of the invention
The invention provides a lactobacillus plantarum, it is strong and effectively can reduce the content of pickle production process nitrite that this bacterial strain produces high, the tolerance of acid amount.
Technical scheme of the present invention:
The invention provides a kind of pickle production method of adding Chinese caterpillar fungus; Comprise the steps:
Take ternip, ginger and garlic mixing and load pickle jar, add the Mixed Microbes powder of vegetable raw-material quality 0.05-0.2%, cover lid, use water seal mouth, guarantee the oxygen-free environment in altar, fermentation temperature is 20-30 DEG C, after fermentation 5-8h, add Chinese caterpillar fungus enzymolysis liquid, continue fermentation 58-80 hour.
Mixed Microbes powder parts by weight consist of: Lactobacillus plantarum 7-10, acetobacter 1.Acetobacter can select commercially available bacterium powder, and acetobacter also can be selected to obtain through fermented and cultured drying.
Chinese caterpillar fungus enzymolysis liquid: square Chinese caterpillar fungus being cut into 1 × 1mm, material-water ratio is 1:10, temperature 55-60 degree, pH6.5, add neutral cellulase (Ningxia jade of the He family Bioisystech Co., Ltd), neutral cellulase addition is the 0.1-1% of Chinese caterpillar fungus quality, and speed of agitator 50-60 turns/min, enzymolysis 3-5h.Then regulate temperature 40 degree, pH is 3.0, and add acid protease (Shandong Su Kehan bioengineering limited company), acid protease addition is the 0.1-1% of Chinese caterpillar fungus quality, and speed of agitator 50-60 turns/min, enzymolysis 2-4h.Chinese caterpillar fungus is Chinese caterpillar fungus.
Feedstock treating: picked by all pickles raw materials, peeling, stripping and slicing is for subsequent use.Clean all pickles raw materials with cold water, comprise, ternip, ginger, garlic etc., drain for subsequent use.
Every day, sampling detected ternip nitrite, found that, from second day, in whole sweat, ternip nitrite is all in below 2.8mg/kg, is starkly lower than the content of nitrite of ternip in traditional pickling process process, meets national standard.Fragrance is stronger, and brittleness is moderate.
Wherein, ternip 40-50: ginger 0.5-1: Chinese caterpillar fungus 1-2: garlic is: 3-10 (ratio of weight and number)
One lactobacillus plantarum tlj-2014, described Lactobacillus plantarum (Lactobacillus plantarum) tlj-2014 is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (being called for short CGMCC) on July 2nd, 2014, preserving number is CGMCC NO.9405, preservation address is: No. 3, No. 1, North Star West Road, Chaoyang District, city of BeiJing, China institute, Institute of Microorganism, Academia Sinica, postcode: 100101.
Described Lactobacillus plantarum tlj-2014 adopts following flow process to carry out seed selection:
The original bacterial classification that sets out → test tube activation → dithyl sulfate (DES) mutagenesis → nitrosoguanidine (NTG) mutagenesis → plasma mutagenesis → dull and stereotyped primary dcreening operation → shaking flask sieves → mitotic stability test again.
Described Lactobacillus plantarum tlj-2014 has following character:
(1) production of lactic acid speed can reach 35g/L/d, and this bacterial strain lactic acid concn after 71 hours fermentation reaches 95g/L;
(2) have acid resistance, when pH 1.80, survival is good;
(3) degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (speed of spontaneous fermentation process nitrite accumulation is approximately 1.1mg/h/kg), can resistance to 1% cholate.
Beneficial effect:
Through experiment, Lactobacillus plantarum tlj-2014 provided by the present invention finds that genetic stability is comparatively strong, inclined-plane can go down to posterity for continuous ten times, and proterties does not have significant change, property indices is all normal; Production of lactic acid speed can reach 35g/L/d, and this bacterial strain lactic acid concn after 71 hours fermentation reaches 95g/L; Can survive under pH is the condition of 1.80, can resistance to 1% cholate; Degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg, this bacterial classification when producing pickles, whole sweat nitrite concentration at below 5mg/kg, far below the content specified in standard GB/T 2714-2003 (20mg/kg).
In experimentation, unexpected discovery is added Chinese caterpillar fungus enzymolysis liquid and is effectively reduced content of nitrite after fermentation 5h, and in whole sweat, ternip nitrite is all in below 2.9mg/kg; The pickles content of nitrite not adding Chinese caterpillar fungus enzymolysis liquid after fermentation 5-8h is in 4.5-5.0mg/kg.
Specific embodiment 1:
Feedstock treating: picked by all pickles raw materials, peeling, stripping and slicing is for subsequent use.Clean all pickles raw materials with cold water, comprise, ternip, ginger, garlic etc., drain for subsequent use.
Chinese caterpillar fungus enzymolysis liquid: square Chinese caterpillar fungus being cut into 1 × 1mm, material-water ratio is 1:10, temperature 55 degree, pH6.5, adds neutral cellulase (Ningxia jade of the He family Bioisystech Co., Ltd) 1000u/g Chinese caterpillar fungus, speed of agitator 300 turns/min, enzymolysis 5.5h.Then regulate temperature 40 degree, pH is 3.0, adds acid protease (Shandong Su Kehan bioengineering limited company) 500u/g Chinese caterpillar fungus, speed of agitator 300 turns/min, enzymolysis 3.5h.
The configuration of Mixed Microbes powder: Lactobacillus plantarum: acetobacter is 10:1 (weight ratio), bacterium powder addition is 0.05-0.2% (bacterium grain weight amount: vegetables weight)
Pickle: take ternip 5kg, to scale (ternip 50: ginger 0.5: fructification 1: garlic 3, weight fraction ratio) and add ginger and garlic mixing loading pickle jar, add Mixed Microbes powder; Cover lid, water seal mouth, guarantees the oxygen-free environment in altar, and fermentation temperature is 25 DEG C, after fermentation 6h, adds Chinese caterpillar fungus enzymolysis liquid, continues fermentation to 60 hours.
Every day, sampling detected ternip nitrite, found that, from second day, in whole sweat, ternip nitrite is all in below 2.8mg/kg, is starkly lower than the content of nitrite of ternip in traditional pickling process process, meets national standard.
Embodiment 2:
Feedstock treating: picked by all pickles raw materials, peeling, stripping and slicing is for subsequent use.Clean all pickles raw materials with cold water, comprise, ternip, ginger, garlic etc., drain for subsequent use.
Cordyceps sinensis enzymolysis liquid: square Cordyceps sinensis being cut into 1 × 1mm, material-water ratio is 1:10, temperature 55 degree, pH6.5, add neutral cellulase (Ningxia jade of the He family Bioisystech Co., Ltd) 1000u/g Chinese caterpillar fungus, speed of agitator 300 turns/min, enzymolysis 5.5h.Then regulate temperature 40 degree, pH is 3.0, adds acid protease (Shandong Su Kehan bioengineering limited company) 500u/g Chinese caterpillar fungus, speed of agitator 300 turns/min, enzymolysis 3.5h.
The configuration of Mixed Microbes powder: Lactobacillus plantarum: acetobacter is 10:1 (weight ratio), bacterium powder addition is 0.1% (bacterium grain weight amount: vegetables weight)
Pickle: take ternip 5kg, to scale (ternip 50: ginger 0.5: fructification 1: garlic 3, weight fraction ratio) and add ginger and garlic mixing loading pickle jar, add Mixed Microbes powder; Cover lid, water seal mouth, guarantees the oxygen-free environment in altar, and fermentation temperature is 25 DEG C, after fermentation 5h, adds Cordyceps sinensis enzymolysis liquid, continues fermentation to 70 hours.
Every day, sampling detected ternip nitrite, found that, from second day, in whole sweat, ternip nitrite is all in below 2.7mg/kg, is starkly lower than the content of nitrite of ternip in traditional pickling process process, meets national standard.
In Tianjin according to Different age group (15-25 year, 26-35 year, 36-45 year and more than 46 years old) choose 50 people respectively and taste, and formulate grade form, statistics, be averaged a point statistics, as shown in the table.The product fragrance that the method is produced is stronger, and brittleness is moderate, and integrated sensory evaluates far above commercially available prod.
Grade form

Claims (6)

1. add a pickle production method for Chinese caterpillar fungus, comprise the steps:
Take ternip, ginger and garlic mixing and load pickle jar, add the Mixed Microbes powder of vegetable raw-material quality 0.05-0.2%, cover lid, use water seal mouth, guarantee the oxygen-free environment in altar, fermentation temperature is 20-30 DEG C, after fermentation 5-8h, add Chinese caterpillar fungus enzymolysis liquid, continue fermentation 58-80 hour; Mixed Microbes powder parts by weight consist of: Lactobacillus plantarum 7-10, acetobacter 1.
2. add the pickle production method of Chinese caterpillar fungus according to claim 1, Chinese caterpillar fungus enzymolysis liquid is prepared as follows: square Chinese caterpillar fungus being cut into 1 × 1mm, material-water ratio is 1:10, temperature 55-60 degree, pH6.5, adds neutral cellulase, and neutral cellulase addition is the 0.1-1% of Chinese caterpillar fungus quality, speed of agitator 50-60 turns/min, enzymolysis 3-5h.Then regulate temperature 40 degree, pH is 3.0, adds acid protease, and acid protease addition is the 0.1-1% of Chinese caterpillar fungus quality, and speed of agitator 50-60 turns/min, enzymolysis 2-4h.Chinese caterpillar fungus is Chinese caterpillar fungus.
3. according to claim 1 or 2, add the pickle production method of Chinese caterpillar fungus, wherein, ternip 40-50: ginger 0.5-1: Chinese caterpillar fungus 1-2: garlic is: 3-10.
4. according to claim 1 meeting 2, add the pickle production method of Chinese caterpillar fungus, described Lactobacillus plantarum (Lactobacillusplantarum) tlj-2014 preserving number is CGMCC NO.9405.
5. add the pickle production method of Chinese caterpillar fungus according to claim 4, described Lactobacillus plantarum tlj-2014 has following character:
(1) production of lactic acid speed can reach 35g/L/d, and this bacterial strain lactic acid concn after 71 hours fermentation reaches 95g/L;
(2) have acid resistance, when pH 1.80, survival is good;
(3) degrading nitrite speed is fast, and capacity of decomposition reaches 9.8mg/h/kg (speed of spontaneous fermentation process nitrite accumulation is approximately 1.1mg/h/kg), can resistance to 1% cholate.
6. according to claim 1 or 2, add the pickle production method of Chinese caterpillar fungus, described in pickle for: take ternip 5kg, to scale ternip 50: ginger 0.5: Chinese caterpillar fungus 1: garlic 3, add ginger and garlic mixing load pickle jar, add Mixed Microbes powder; Cover lid, water seal mouth, guarantees the oxygen-free environment in altar, and fermentation temperature is 25 DEG C, after fermentation 6h, adds Chinese caterpillar fungus enzymolysis liquid, continues fermentation to 60 hours.
CN201510011862.9A 2015-01-09 2015-01-09 Preparation method of pickle containing cordyceps sinensis Pending CN104585681A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110881635A (en) * 2019-12-27 2020-03-17 华南师范大学 Method for inhibiting accumulation of nitrite in pickle by cordyceps militaris and application of method
CN114586956A (en) * 2022-01-19 2022-06-07 青岛农业大学 A pickled vegetable containing cordycepin and its preparation method

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102899262A (en) * 2012-05-31 2013-01-30 浙江正味食品有限公司 Lactobacillus plantarum and method for fast degrading nitrite produced in fermentation by the lactobacillus plantarum
CN103027290A (en) * 2012-12-21 2013-04-10 中国农业大学 Application of boletus edulis in reducing nitrite content in fermented food or pickled food

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102899262A (en) * 2012-05-31 2013-01-30 浙江正味食品有限公司 Lactobacillus plantarum and method for fast degrading nitrite produced in fermentation by the lactobacillus plantarum
CN103027290A (en) * 2012-12-21 2013-04-10 中国农业大学 Application of boletus edulis in reducing nitrite content in fermented food or pickled food

Non-Patent Citations (2)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110881635A (en) * 2019-12-27 2020-03-17 华南师范大学 Method for inhibiting accumulation of nitrite in pickle by cordyceps militaris and application of method
CN114586956A (en) * 2022-01-19 2022-06-07 青岛农业大学 A pickled vegetable containing cordycepin and its preparation method

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Application publication date: 20150506