CN104585475A - Method for preparing soluble protein powder by marine fish wastes - Google Patents
Method for preparing soluble protein powder by marine fish wastes Download PDFInfo
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- CN104585475A CN104585475A CN201510013330.9A CN201510013330A CN104585475A CN 104585475 A CN104585475 A CN 104585475A CN 201510013330 A CN201510013330 A CN 201510013330A CN 104585475 A CN104585475 A CN 104585475A
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Abstract
The invention relates to a method for preparing soluble protein powder by marine fish wastes, and the method is reasonable in collocation and working procedures, high in production efficiency and good in output effect. The method comprises the following steps: 1, cleaning the fish wastes, feeding water into the cleaned fish wastes and mashing to obtain fish cream; putting the obtained fish cream into a reaction vessel, and feeding water to adjust the pH value to be 6.5-7.5; 2, feeding protease into the reaction vessel, wherein the weight of the protease is 1-2% of that of the total weight of the raw material; evenly mixing, and carrying out a reaction at the reaction temperature of 45-60 DEG C for 2-4 hours; 3, heating, boiling, and maintaining the constant temperature to be 100 DEG C for 10-15 minutes to enable the activity of enzyme to be lost; 4, carrying out centrifugal separation, and filtering out waste residue to obtain a fish albumen hydrolysis solution; 5, feeding activated carbon into the fish albumen hydrolysis solution, and carrying out decoloration at the temperature of 60-65 DEG C for 30 minutes, wherein the dosage of the activated carbon is 10g/ L; 6, after decoloration, carrying out vacuum drying at the temperature of 50-55 DEG C; and 7, grinding the dried solid to obtain the soluble protein powder.
Description
Technical field
The present invention relates to a kind of production method of ocean fish leftover bits and pieces soluble protein powder.
Background technology
Along with the rise of albumen powder goods, people more and more recognize the importance of peptide, and in the flesh of fish, crude protein content is up to more than 15%, and contained necessary amino acid is complete.Functional areas at the inner ubiquity of the polypeptide chain of Fish protein, select these polypeptide chains of suitable protease hydrolytic, by the method that present property enzymolysis is dry again, just likely prepare the fish protein powder product containing various physiologically active peptide section by Fish protein.But existing production technology is unsatisfactory in the control of temperature, time.
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of production method of the ocean fish leftover bits and pieces soluble protein powder reasonable, operation is reasonable, production efficiency is high, output is effective of arranging in pairs or groups.
For achieving the above object, technical scheme of the present invention is: a kind of production method of ocean fish leftover bits and pieces soluble protein powder, comprises the following steps:
The first step, cleans the leftover bits and pieces of fish, adds water and smash to pieces, obtains fish slurry, and get the fish slurry smashed to pieces in reaction pot, adding water is adjusted to 6.5 ~ 7.5 by pH value;
Second step, adds the protease of gross weight 1% ~ 2%, stirs in reaction pot, reacts 2 ~ 4 hours under the reaction temperature of 45 DEG C ~ 60 DEG C;
3rd step, heating is boiled and constant temperature to 100 degree Celsius, and constant temperature 10 ~ 15 minutes, makes enzyme lose activity;
4th step, through centrifugation, elimination waste residue, obtains fish albumen hydrolysis solution;
5th step, in fish albumen hydrolysis solution, add active carbon decolour 30 minutes at the temperature of 60 ~ 65 DEG C, activated carbon dosage is 10g/L;
6th step, after decolouring, vacuum drying at the temperature of 50 ~ 55 DEG C;
7th step, by dried solid through pulverizing to obtain soluble protein powder.
Preferably, in second step, protease adopts alkali protease.
Preferably, in described step one, add the purple perilla liquid of gross weight 4 ~ 5%, the vinegar of gross weight 5 ~ 10 ‰, the ethyl maltol of gross weight 5 ~ 10 ‰ be used for raw meat.
Technique scheme has following beneficial effect: the raw material of fish protein powder prepared by the present invention is low-value small fish, fish head, fish tail, wide material sources, effectively facilitate the exploitation in low-value freshwater fish market, make low-value freshwater fish product more diversified, meet the market demand of people to freshwater fish products and various fish protein, higher value application for fresh-water fishes provides a new approach, utilize biological enzymolysis technology, fish protein is hydrolyzed more thorough, the fish protein powder nutrition obtained is abundanter, product safety, and have no side effect, can be used as foodstuff base material exploitation, there are wide market prospects, further, be used for raw meat by the collocation of purple perilla liquid, vinegar, ethyl maltol, go raw meat effect better.
Detailed description of the invention
Below in conjunction with concrete embodiment, the present invention is described in further detail.
The present embodiment provides a kind of production method of ocean fish leftover bits and pieces soluble protein powder, comprises the following steps:
The first step, cleans the leftover bits and pieces of fish, adds water and smash to pieces, obtains fish slurry, gets the fish slurry smashed to pieces in reaction pot, add the purple perilla liquid of gross weight 5%, the vinegar of gross weight 5 ‰, gross weight 5 ‰ ethyl maltol for removing raw meat, adding water is adjusted to 6.5 by pH value;
Second step, adds the alkali protease of gross weight 1%, stirs in reaction pot, reacts 4 hours under the reaction temperature of 45 DEG C;
3rd step, heating is boiled and constant temperature to 100 degree Celsius, and constant temperature 15 minutes, makes enzyme lose activity;
4th step, through centrifugation, elimination waste residue, obtains fish albumen hydrolysis solution;
5th step, in fish albumen hydrolysis solution, add active carbon decolour 30 minutes at the temperature of 60 DEG C, activated carbon dosage is 10g/L;
6th step, after decolouring, vacuum drying at the temperature of 50 DEG C;
7th step, by dried solid through pulverizing to obtain soluble protein powder.
Wherein in the first step, pH value can get arbitrary numerical value in the scope of 6.5 to 7.5, the numerical value of purple perilla liquid can get the arbitrary numerical value between gross weight 4 ~ 5%, the numerical value of vinegar can get the arbitrary numerical value between gross weight 5 ~ 10 ‰, and the numerical value of ethyl maltol can get the arbitrary numerical value between gross weight 5 ~ 10 ‰; Wherein in second step protease for arbitrary numerical value can be got in 1% ~ 2%, and reaction temperature can be selected arbitrarily in 40 ~ 60 DEG C, and the reaction time can get the arbitrary numerical value in 2 ~ 4 hours; Wherein in the 3rd step, thermostat temperature can get arbitrary numerical value in 10 ~ 15 minutes; In 5th step, the temperature of decolouring controls the arbitrary numerical value in 60 ~ 65 DEG C; In 6th step, baking temperature can get the arbitrary numerical value between 50 ~ 55 DEG C.
The raw material of fish protein powder prepared by the present invention is low-value small fish, fish head, fish tail, wide material sources, effectively facilitate the exploitation in low-value freshwater fish market, make low-value freshwater fish product more diversified, meet the market demand of people to freshwater fish products and various fish protein, higher value application for fresh-water fishes provides a new approach, utilize biological enzymolysis technology, fish protein is hydrolyzed more thorough, the fish protein powder nutrition obtained is more abundant, product safety and having no side effect, can be used as foodstuff base material exploitation, there are wide market prospects; Further, be used for raw meat by the collocation of purple perilla liquid, vinegar, ethyl maltol, go raw meat effect better.
Below be only the present invention's preferred embodiment, those skilled in the art makes by claim the protection domain that equivalent change all falls into this case.
Claims (3)
1. a production method for ocean fish leftover bits and pieces soluble protein powder, is characterized in that, comprise the following steps:
The first step, cleans the leftover bits and pieces of fish, adds water and smash to pieces, obtains fish slurry, and get the fish slurry smashed to pieces in reaction pot, adding water is adjusted to 6.5 ~ 7.5 by pH value;
Second step, adds the protease of gross weight 1% ~ 2%, stirs in reaction pot, reacts 2 ~ 4 hours under the reaction temperature of 45 DEG C ~ 60 DEG C;
3rd step, heating is boiled and constant temperature to 100 degree Celsius, and constant temperature 10 ~ 15 minutes, makes enzyme lose activity;
4th step, through centrifugation, elimination waste residue, obtains fish albumen hydrolysis solution;
5th step, in fish albumen hydrolysis solution, add active carbon decolour 30 minutes at the temperature of 60 ~ 65 DEG C, activated carbon dosage is 10g/L;
6th step, after decolouring, vacuum drying at the temperature of 50 ~ 55 DEG C;
7th step, by dried solid through pulverizing to obtain soluble protein powder.
2. the production method of a kind of ocean fish leftover bits and pieces soluble protein powder according to claim 1, is characterized in that: in second step, and protease adopts alkali protease.
3. the production method of a kind of ocean fish leftover bits and pieces soluble protein powder according to claim 1, it is characterized in that: in described step one, add the purple perilla liquid of gross weight 4 ~ 5%, the vinegar of gross weight 5 ~ 10 ‰, the ethyl maltol of gross weight 5 ~ 10 ‰ be used for raw meat.
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CN201510013330.9A CN104585475A (en) | 2015-01-12 | 2015-01-12 | Method for preparing soluble protein powder by marine fish wastes |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ITUA20163854A1 (en) * | 2016-05-27 | 2017-11-27 | Racof S R L | PROCESS FOR REALIZING A PROTEIC HYDROLYZATE WITH "NUTRACEUTICAL" FUNCTIONAL CHARACTERISTICS OBTAINED FROM THE WASTE OF ANIMAL SLAUGHTER PROCESSING. |
CN107541539A (en) * | 2017-09-01 | 2018-01-05 | 浦江县欧立生物技术有限公司 | A kind of preparation method of ocean fish leftover protein powder |
CN107996819A (en) * | 2017-12-14 | 2018-05-08 | 荣成市飞创科技有限公司 | A kind of method using marine products discarded object extraction protein oral liquor |
Citations (4)
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CN1943365A (en) * | 2006-11-02 | 2007-04-11 | 天津市诺奥科技发展有限公司 | Process for hydrolyzing fish processing leftover using emzyme preparation |
CN102318721A (en) * | 2011-06-16 | 2012-01-18 | 天津科技大学 | Preparation method of deodorized fish protein powder |
CN103284122A (en) * | 2012-02-27 | 2013-09-11 | 熊新安 | Whitebait sauce and preparation method thereof |
CN103284230A (en) * | 2013-06-17 | 2013-09-11 | 钦州市钦州港永健水产贸易有限公司 | Processing method of clam roasted slices |
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2015
- 2015-01-12 CN CN201510013330.9A patent/CN104585475A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1943365A (en) * | 2006-11-02 | 2007-04-11 | 天津市诺奥科技发展有限公司 | Process for hydrolyzing fish processing leftover using emzyme preparation |
CN102318721A (en) * | 2011-06-16 | 2012-01-18 | 天津科技大学 | Preparation method of deodorized fish protein powder |
CN103284122A (en) * | 2012-02-27 | 2013-09-11 | 熊新安 | Whitebait sauce and preparation method thereof |
CN103284230A (en) * | 2013-06-17 | 2013-09-11 | 钦州市钦州港永健水产贸易有限公司 | Processing method of clam roasted slices |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ITUA20163854A1 (en) * | 2016-05-27 | 2017-11-27 | Racof S R L | PROCESS FOR REALIZING A PROTEIC HYDROLYZATE WITH "NUTRACEUTICAL" FUNCTIONAL CHARACTERISTICS OBTAINED FROM THE WASTE OF ANIMAL SLAUGHTER PROCESSING. |
EP3248469A1 (en) * | 2016-05-27 | 2017-11-29 | Racof S.r.l. | Process for making a protein hydrolysate with "nutraceutical" functional features obtained from process waste of slaughterhouses |
CN107541539A (en) * | 2017-09-01 | 2018-01-05 | 浦江县欧立生物技术有限公司 | A kind of preparation method of ocean fish leftover protein powder |
CN107996819A (en) * | 2017-12-14 | 2018-05-08 | 荣成市飞创科技有限公司 | A kind of method using marine products discarded object extraction protein oral liquor |
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