CN104568879B - A kind of carbendazim detection method based on fluorescence probe - Google Patents
A kind of carbendazim detection method based on fluorescence probe Download PDFInfo
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Abstract
The invention provides a kind of carbendazim detection method based on fluorescence probe, comprise the following steps：Fluorescence probe is dissolved with organic solvent, is configured to solution A；Prepare aqueous slkali B；Carbendazim standard liquid is prepared, adds solution A and solution B thereto, detects fluorescence signal intensity, draws the fluorescence intensity of carbendazim standard liquid and the standard curve of concentration relationship；Testing sample solution is prepared, adds solution A and solution B thereto, detects fluorescence signal intensity, the content of carbendazim in sample is obtained according to obtained standard curve.The present invention occurs specific reaction by fluorescence probe and carbendazim and produces fluorescence signal, by the intensity for detecting fluorescence signal, to determine the content of carbendazim in sample according to the relation of fluorescence signal intensity and concentration, method is simple, cost is low, carbendazim content can be detected in the basic conditions, there is high specific, high sensitivity, accurately and reliably, available for the carbendazim content in quick detection fruits and vegetables.
Description
Technical field
The invention belongs to Food Safety Analysis technical field, is related to a kind of detection method of carbendazim, more particularly to a kind of
Carbendazim detection method based on fluorescence probe.
Background technology
Carbendazim (carbendazim), it is a kind of benzimidazoles residues, while is also that benomyl (benomyl) is being fed
Main metabolites in newborn animal body and catabolite in the environment.Carbendazim is wide as a kind of bactericide of broad-spectrum high efficacy
The general powdery mildew for being applied to preventing and treating each agricultural products such as vegetables and fruit and downy mildew etc., it is to people, animal low toxicity, if but long-term
Intake, can in vivo be accumulated, influence health so as to cause chronic or acute toxicity, and excitement can be caused by esophagus, is taken out
Jerk, be absent-minded, nausea and vomiting, dizzy headache, the poisoning symptom such as uncomfortable in chest, upper abdomen tenderness.
Current existing carbendazim detection means more dependent on gas phase, liquid chromatogram and its and mass spectrometric hyphenated technique, detection
Cycle is grown, and program is complicated, and required instrument cost is expensive, and the requirement to operating personnel is high, and limiting it can only be in specialized laboratory
Use, it is difficult to timely, convenient, economically carry out large-scale sample screening and the monitoring of agricultural product food production.Therefore
Seek quick, convenient, accurate and economically viable detection method and turned into urgent need to resolve in carbendazim retention analysis research to ask
Topic.
CN 103472122A disclose a kind of electrochemical sensor detection method for detecting the how clever bacterium agricultural chemicals of trace, described
Method comprises the following steps：1. in 100mL water plus 80 DEG C of 2h are heated in 0.2~2g montmorillonites；2. in 100mL water plus 0.2g is adjacent
Phthalic acid diethylene glycol diacrylate, add 1g NaCl, pH 9-10 are adjusted with 0.1mol/L NaOH solutions；3. by 2 solution
Mixed with 1 constant temperature to 60 DEG C, Cheng Fen；4. glass-carbon electrode is polished, with acetone, ethanol, and 10%NaOH solution, 1:1 HNO3, water is clear
Wash；5. by 4 glass-carbon electrode with 50mVS-1Scanning；6. take 0.005g PDDAs to be modified to cover
De- soil, obtains sensor；7. sensor is placed in carbendazim solution, B-R buffer solutions are added, are detected with differential pulse voltammetry.Should
Although method can detect the how clever bacterium agricultural chemicals of trace, the method is more complicated, cycle length, takes time and effort, so applying
Middle tool has certain limitations.
Jiang Xintian and Ding Ming was studied in the fluorescence spectrometry to carbendazim in 1989, and its research is to be based on carbendazim
There is very strong transmitting fluorescence in acid medium, and the non-blooming property in alkaline medium.But more bacterium in our current research
The fluorescence of spirit is easily influenceed by factors such as acidity, temperature and reagents, unstable so that the standard of carbendazim quantified results
Exactness is not high, and is only suitable for the detection of carbendazim under acid condition, is not suitable for alkalescence condition.
CN 103175813A disclose the side of carbendazim and probenazole content in fluorescence spectrum Rapid Simultaneous Determination vegetables
Method, this invention are pre-processed by simple extraction step to sample, then collect the three-dimensional excitation-emission fluorescence light of sample
Spectrum, and the spectroscopic data to obtaining is pre-processed i.e. deduction Rayleigh scattering and Raman scattering, the data after processing and tieed up along sample
Direction stacks gradually, and obtains a three-dimensional data battle array；Then the parallel factor analysis algorithm in Applied Chemometrics is
PARAFAC parses to the three-dimensional data battle array, produces relative excitation spectrum matrix A, relative emission spectra matrix B and relatively dense
Spend Matrix C, by relative concentration matrix with two kinds of analytes corresponding to relative concentration vector and corresponding mark in correction sample
Quasi- concentration makees linear regression, obtains calibration model, and the three-dimensional excitation-emission of each sample is then collected under identical experiment parameter
Matrix fluorescence spectrum, it is that PARAFAC is handled the data obtained equally using parallel factor analysis algorithm, will differentiates what is obtained
Relative concentration is input to calibration model, and the prediction of corrected model obtains the concentration of carbendazim and probenazole in each sample and contained
Amount.Although the method quantitatively detects while can realizing two kinds of target agricultural chemicals in vegetables, model establishes process and receipts
The process for collecting the three-dimensional Excitation-emission matrix fluorescence spectrum of each sample is still more complicated.
In recent years, fluorescent probe technique is applied widely in fields such as medicine, food, health, in material detection side
Method, using fluorescence probe there is the property of specific fluorescent can realize target detection to be measured, can also utilize fluorescence probe with
The chemical reaction of high specific occurs for object to be measured, and produces highly sensitive fluorescence signal, micro- for qualitative and quantitative detection
Amount or trace object to be measured.This method has the features such as cost is relatively low, high to external environmental condition tolerance degree.
Therefore, it is desirable to develop a kind of carbendazim quick determination method based on fluorescence probe in this area.
The content of the invention
In view of the shortcomings of the prior art, it is an object of the invention to provide a kind of carbendazim detection method, especially it is to provide
A kind of carbendazim detection method based on fluorescence probe.
To reach this goal of the invention, the present invention uses following technical scheme：
The invention provides a kind of carbendazim detection method based on fluorescence probe, comprise the following steps：
(1) fluorescence probe is dissolved with organic solvent, is configured to solution A；
(2) aqueous slkali B is prepared；
(3) carbendazim standard liquid is prepared, adds solution A and solution B thereto, utilizes spectrophotometer detection fluorescence letter
Number intensity, draw the fluorescence intensity of carbendazim standard liquid and the standard curve of concentration relationship；
(4) testing sample solution is prepared, adds solution A and solution B thereto, fluorescence signal is detected using spectrophotometer
Intensity, the standard curve obtained according to step (3) obtain the content of carbendazim in sample.
In the carbendazim detection method of the present invention based on fluorescence probe, step (1) described fluorescence probe is phenanthrenequione；
Preferably, step (1) described fluorescence probe is 9,10- phenanthrenequione.
In the carbendazim detection method of the present invention based on fluorescence probe, step (1) described organic solvent is diformazan
Base sulfoxide (DMSO) or N,N-dimethylformamide (DMF)；The concentration of step (1) described solution A is 400-600ppm, such as
400ppm、420ppm、440ppm、460ppm、480ppm、500ppm、520ppm、540ppm、550ppm、560ppm、580ppm、
590ppm or 600ppm, preferably 500ppm.
In the carbendazim detection method of the present invention based on fluorescence probe, step (2) described aqueous slkali can be hydrogen
Sodium hydroxide solution or potassium hydroxide solution.The concentration of step (2) described aqueous slkali is 0.5-1.5mol/L, such as 0.5mol/L,
0.6mol/L、0.7mol/L、0.8mol/L、0.9mol/L、1.0mol/L、1.1mol/L、1.2mol/L、1.3mol/L、
1.4mol/L or 1.5mol/L, preferably 1mol/L.
In the carbendazim detection method of the present invention based on fluorescence probe, step (3) is described to prepare carbendazim standard
Solution solvent for use is deionized water.Step (3) concentration gradient for preparing the carbendazim that carbendazim standard liquid is selected
For 0.01ppm, 0.05ppm, 0.2ppm, 1ppm, 5ppm, 25ppm, 100ppm.Step (3) solution A and carbendazim standard
The mol ratio of solution is more than 1, such as solution A and the mol ratio of carbendazim standard liquid can be 1.5:1、2:1、3:1、4:1、6:
1、8:1、10:1、20:1、25:1、30:1、40:1、50:1、100:1、200:1、400:1、800:1、1000:1、2000:1、
4000:1、6000:1、8000:1、10000:1 etc.；Preferably, relative to 1.0mL carbendazim standard liquids, the addition of solution B
For 0.1-0.3mL, for example, 0.1mL, 0.13mL, 0.15mL, 0.17mL, 0.18mL, 0.2mL, 0.22mL, 0.24mL, 0.26mL,
0.28mL or 0.3mL；Preferably, it is molten to be equal to carbendazim standard used in step (3) for the volume of testing sample solution used in step (4)
The volume of liquid；Preferably, the solution A and solution B with step (3) moderate are added in step (4).
As optimal technical scheme, the carbendazim detection method of the present invention based on fluorescence probe, specifically include with
Lower step：
(1) by fluorescence probe 9,10- phenanthrenequione dmso solutions, it is configured to 500ppm solution A；
(2) 1mol/L sodium hydroxide solutions B is prepared；
(3) prepare carbendazim standard liquid, the concentration gradient of the carbendazim select be 0.01ppm, 0.05ppm,
0.2ppm, 1ppm, 5ppm, 25ppm, 100ppm, take 1.0mL carbendazim standard liquids, thereto add 0.25mL solution As and
0.3mL solution Bs, fluorescence signal intensity is detected, draw the fluorescence intensity of carbendazim standard liquid and the standard curve of concentration relationship；
(4) testing sample solution is prepared, takes 1.0mL testing sample solutions, adds 0.25mL solution As and 0.3mL thereto
Solution B, fluorescence signal intensity is detected, the standard curve obtained according to step (3) obtains the content of carbendazim in sample.
The fluorescence probe is phenanthrenequione in the present invention, although phenanthrenequione itself has fluorescence, it is anti-with carbendazim
Should after, maximum emission wavelength can drift about, after being reacted with carbendazim by determining it specific wavelength (such as 9,
After the reaction of 10- phenanthrenequione and carbendazim maximum emission wavelength is in 333nm) fluorescence intensity measure the content of carbendazim, so
Test fluorescence signal intensity refers to testing fluorescence probe phenanthrenequione and the fluorescence intensity after carbendazim reaction, institute in the present invention
The accuracy and sensitivity of the detection method will not be had an impact with the fluorescence of its own.
In addition, the present invention is to detect the content of carbendazim in the basic conditions, because carbendazim itself is in the basic conditions
Fluorescence is not produced, and it is with that can produce fluorescence in the basic conditions after phenanthrenequione reaction, so by ensureing to add phenanthrene
The mole of quinone is more than the mole of carbendazim, it is ensured that carbendazim reacts with phenanthrenequione completely in sample, and then can be with
The accurate content for detecting carbendazim in sample.
The present invention is using fluorescence probe and object carbendazim specific reaction to be measured and generates new fluorescence-causing substance, the product
There is maximum fluorescence intensity in 333nm transmitted wave strong points, it is true by the intensity of product fluorescence signal and the relation of carbendazim concentration
The content of carbendazim in random sample product, this method is fairly simple, can be with more in a variety of fruits and vegetables such as quick detection tomato, citrus, apple
The content of bacterium spirit.
Relative to prior art, the invention has the advantages that：
The invention provides a kind of carbendazim detection method based on fluorescence probe, visited in the methods of the invention by fluorescence
Pin occurs specific reaction with carbendazim and produces fluorescence signal, strong according to fluorescence signal by detecting the intensity of fluorescence signal
The relation of degree and concentration determines the content of carbendazim in sample, and method is simple, and cost is low, can detect carbendazim in the basic conditions
Content, there is high specific, high sensitivity, accurately and reliably, available for the carbendazim content in quick detection fruits and vegetables.
Brief description of the drawings
Fig. 1 is the fluorescence spectra that carbendazim detects in the embodiment of the present invention 1；
Fig. 2 is the carbendazim concentration that the embodiment of the present invention 1 is drawn and the canonical plotting of fluorescence intensity.
Embodiment
Technical scheme is further illustrated below by embodiment.Those skilled in the art should be bright
, the embodiment be only to aid in understand the present invention, be not construed as to the present invention concrete restriction.
The detection of carbendazim content in the tomato of embodiment 1
The preparation of tomato sample
Tomato former state 10g is weighed, adds 0.1g sodium carbonate and 20mL ethyl acetate, whirlpool mixes ultrasonic 25min after 1min,
10mL supernatants are taken to be dried up in 45 DEG C of water-baths with faint nitrogen after centrifugation, it is with 2mL0.05mol/L dissolving with hydrochloric acid residue in case net
Change；
Solid phase extraction column (OasisMCX, 60mg, 3cc) is carried out with 2mL methanol, 2mL 0.15mol/L ammoniacal liquor successively
Use front activating；By the tomato sample loading after above-mentioned dissolving with hydrochloric acid to solid phase extraction column, successively with 1mL 0.15mol/L ammonia
Water, 1mL ammoniacal liquor (0.15mol/L)-methanol solution, 1mL 0.1mol/L hydrochloric acid, 1mL methanol elution pillar, discard elution outflow
Liquid, the flow control of whole lessivation is within 3mL/min；Finally with 2mL ammoniacal liquor (0.3mol/L)-methanol solution elution post
Son, eluent is collected, be heated to 45 DEG C and redissolved with after the drying of faint nitrogen with the methanol-waters of 1mL 2%, tomato sample is made.
Detect carbendazim content in tomato sample
(1) 12.5mg 9 is weighed, 10- phenanthrenequione is dissolved with a small amount of DMSO, the constant volume in 25mL brown volumetric flasks, is configured to
500ppm solution As are standby；
(2) use deionized water dissolving 400mg sodium hydroxides, the constant volume in 10mL volumetric flasks, be configured to 1mol/L hydrogen-oxygen
It is standby to change sodium water solution B；
(3) carbendazim standard liquid is prepared：With deionized water prepare 0.01ppm, 0.05ppm, 0.2ppm, 1ppm, 5ppm,
The 25ppm and 100ppm carbendazim aqueous solution, as standard liquid.Sequentially added in 1.5mL centrifuge tubes 0.25mL solution As,
0.3mL solution Bs and 1.0mL carbendazim standard products, 30s is fully vibrated, stand 1min, be placed in quartz colorimetric utensil, in fluorescence point
Under light photometer, using 296nm as excitation wavelength, the maximum fluorescence intensity (as shown in Figure 1) at 333nm is detected, is drawn more
The fluorescence intensity of bacterium spirit standard liquid and the standard curve (as shown in Figure 2) of concentration relationship；
(4) the tomato sample that 0.25mL solution As, 0.3mL solution Bs and 1.0mL are handled well is sequentially added in 1.5mL centrifuge tubes
Product, 30s is fully vibrated, stand 1min, be placed in quartz colorimetric utensil, under sepectrophotofluorometer, excitation wave is used as using 296nm
It is long, the maximum fluorescence intensity at 333nm is detected, the content of carbendazim in sample is obtained according to the standard curve that step (3) is drawn
(because in pretreatment process equivalent to having carried out 10 times of concentrations to sample, in gained sample in carbendazim concentration=test sample
Carbendazim content × 10).
As shown in Figure 1, fluorescence probe 9,10- phenanthrenequione at wavelength 333nm with after carbendazim reaction, having maximum fluorescence strong
Degree, thus reads florescent intensity value under wavelength, is matched with the concentration of carbendazim in standard liquid, can be depicted as the glimmering of carbendazim
Luminous intensity and concentration relationship standard curve, as shown in Figure 2.
As shown in Figure 2, the linear relationship of standard curve obtained by the method for the present invention is preferable, coefficient correlation 0.9593,
It disclosure satisfy that actually detected needs.
For the reliability of verification method, tomato sample 1#, 2# and 3# are prepared as described above, more bacterium are added in each sample
Spirit, recovery of standard addition experiment is carried out, when mark-on amount is 0.05ppm, 0.1ppm and 0.25ppm, the rate of recovery is respectively 80.2%,
85.7% and 83.8%.
From result, more bacterium in the recovery of standard addition experiment of carbendazim in tomato sample are carried out using the method for the present invention
The clever rate of recovery is in 80.2-85.7%, it was demonstrated that this method has the higher degree of accuracy and reliability.
The detection of carbendazim content in the citrus of embodiment 2
The preparation of citrus sample
Citrus former state 10g is weighed, citrus is pre-processed and purified as former state using method in the same manner as in Example 1,
Citrus sample is made.
Detect carbendazim content in citrus sample
(1) 10mg 9 is weighed, 10- phenanthrenequione is dissolved with a small amount of DMF, the constant volume in 25mL brown volumetric flasks, is configured to
400ppm solution As are standby；
(2) use deionized water dissolving 200mg sodium hydroxides, the constant volume in 10mL volumetric flasks, be configured to 0.5mol/L hydrogen
Aqueous solution of sodium oxide B is standby；
(3) carbendazim standard liquid is prepared：With deionized water prepare 0.01ppm, 0.05ppm, 0.2ppm, 1ppm, 5ppm,
The 25ppm and 100ppm carbendazim aqueous solution, as standard liquid.Sequentially added in 2.0mL centrifuge tubes 0.5mL solution As,
0.2mL solution Bs and 1.0mL carbendazim standard products, 30s is fully vibrated, stand 1min, be placed in quartz colorimetric utensil, in fluorescence point
Under light photometer, using 296nm as excitation wavelength, the maximum fluorescence absorption intensity at 330nm is detected, draws carbendazim standard
The fluorescence intensity of solution and the standard curve of concentration relationship；
(4) the citrus sample that 0.5mL solution As, 0.2mL solution Bs and 1.0mL are handled well is sequentially added in 2.0mL centrifuge tubes
Product, 30s is fully vibrated, stand 1min, be placed in quartz colorimetric utensil, under sepectrophotofluorometer, excitation wave is used as using 296nm
It is long, the maximum fluorescence intensity at 333nm is detected, the content of carbendazim in sample is obtained according to the standard curve that step (3) is drawn
(because in pretreatment process equivalent to having carried out 10 times of concentrations to sample, in gained sample in carbendazim concentration=test sample
Carbendazim content × 10).
From result, fluorescence probe 9,10- phenanthrenequione at wavelength 333nm with after carbendazim reaction, having maximum fluorescence
Intensity, florescent intensity value thus is read under wavelength, matched with the concentration of carbendazim in standard liquid, carbendazim can be depicted as
Fluorescence intensity and concentration relationship standard curve.The linear relationship of standard curve obtained by the method for the present invention is preferable, coefficient correlation
For 0.9795, actually detected needs are disclosure satisfy that.
For the reliability of verification method, citrus sample 1#, 2# and 3# are prepared as described above, more bacterium are added in each sample
Spirit, recovery of standard addition experiment is carried out, when mark-on amount is 0.25ppm, 0.5ppm and 1ppm, the rate of recovery is respectively 83.1%,
85.3% and 86.7%.
Therefore, carbendazim in the recovery of standard addition experiment of carbendazim in citrus sample is carried out using the method for the present invention to reclaim
Rate is in 83.1-86.7%, it was demonstrated that this method has the higher degree of accuracy and reliability.
The detection of carbendazim content in the apple of embodiment 3
The preparation of apple sample
Apple former state 10g is weighed, apple is pre-processed and purified as former state using method in the same manner as in Example 1,
Apple sample is made.
Detect carbendazim content in apple sample
(1) 15mg 9 is weighed, 10- phenanthrenequione is dissolved with a small amount of DMSO, the constant volume in 25mL brown volumetric flasks, is configured to
600ppm solution As are standby；
(2) use deionized water dissolving 600mg sodium hydroxides, the constant volume in 10mL volumetric flasks, be configured to 1.5mol/L hydrogen
Aqueous solution of sodium oxide B is standby；
(3) carbendazim standard liquid is prepared：With deionized water prepare 0.01ppm, 0.05ppm, 0.2ppm, 1ppm, 5ppm,
The 25ppm and 100ppm carbendazim aqueous solution, as standard liquid.Sequentially added in 2.0mL centrifuge tubes 0.5mL solution As,
0.1mL solution Bs and 1.0mL carbendazim standard products, 30s is fully vibrated, stand 1min, be placed in quartz colorimetric utensil, in fluorescence point
Under light photometer, using 296nm as excitation wavelength, the maximum fluorescence absorption intensity at 330nm is detected, draws carbendazim standard
The fluorescence intensity of solution and the standard curve of concentration relationship；
(4) the apple sample that 0.5mL solution As, 0.1mL solution Bs and 1.0mL are handled well is sequentially added in 2.0mL centrifuge tubes
Product, 30s is fully vibrated, stand 1min, be placed in quartz colorimetric utensil, under sepectrophotofluorometer, excitation wave is used as using 296nm
It is long, the maximum fluorescence intensity at 333nm is detected, the content of carbendazim in sample is obtained according to the standard curve that step (3) is drawn
(because in pretreatment process equivalent to having carried out 10 times of concentrations to sample, in gained sample in carbendazim concentration=test sample
Carbendazim content × 10).
From result, fluorescence probe 9,10- phenanthrenequione at wavelength 333nm with after carbendazim reaction, having maximum fluorescence
Intensity, florescent intensity value thus is read under wavelength, matched with the concentration of carbendazim in standard liquid, carbendazim can be depicted as
Fluorescence intensity and concentration relationship standard curve.The linear relationship of standard curve obtained by the method for the present invention is preferable, coefficient correlation
For 0.9699, actually detected needs are disclosure satisfy that.
For the reliability of verification method, apple sample 1#, 2# and 3# are prepared as described above, more bacterium are added in each sample
Spirit, recovery of standard addition experiment is carried out, when mark-on amount is 0.5ppm, 1ppm and 2ppm, the rate of recovery is respectively 85.2%, 84.3% and
88.9%.
Therefore, carbendazim in the recovery of standard addition experiment of carbendazim in apple sample is carried out using the method for the present invention to reclaim
Rate is in 84.3-88.9%, it was demonstrated that this method has the higher degree of accuracy and reliability.
The present invention occurs specific reaction by using fluorescence probe and carbendazim and produces fluorescence signal, by detecting fluorescence
The intensity of signal, can be in alkalescence condition to determine the content of carbendazim in sample according to the relation of fluorescence signal intensity and concentration
Lower detection carbendazim content, method is simple, and cost is low, has the characteristics that high specific, high sensitivity, accurately and reliably, can be used for
Carbendazim content in quick detection fruits and vegetables.
Applicant states that the present invention illustrates the carbendazim detection method of the present invention, but the present invention by above-described embodiment
Above method step is not limited to, that is, does not mean that the present invention has to rely on above method step and could implemented.Affiliated technology
The technical staff in field is it will be clearly understood that any improvement in the present invention, to the equivalence replacement and auxiliary of raw material selected by the present invention
The addition of composition, selection of concrete mode etc., within the scope of all falling within protection scope of the present invention and disclosing.
Claims (7)
1. a kind of carbendazim detection method based on fluorescence probe, it is characterised in that comprise the following steps：
(1) fluorescence probe is dissolved with organic solvent, is configured to solution A；
(2) aqueous slkali B is prepared；
(3) carbendazim standard liquid is prepared, adds solution A and solution B thereto, detects fluorescence signal intensity, draws carbendazim
The fluorescence intensity of standard liquid and the standard curve of concentration relationship；
(4) testing sample solution is prepared, adds solution A and solution B thereto, detects fluorescence signal intensity, is obtained according to step (3)
To standard curve obtain sample in carbendazim content；
Step (1) described fluorescence probe is 9,10- phenanthrenequione；Step (2) described aqueous slkali is sodium hydroxide solution or potassium hydroxide
Solution, the concentration of step (2) described aqueous slkali is 0.5-1.5mol/L；Step (1) described organic solvent be dimethyl sulfoxide (DMSO) or
DMF, the concentration of step (1) described solution A is 400-600ppm, and step (3) is described to prepare carbendazim standard
Solution solvent for use is deionized water, and step (3) solution A and the mol ratio of carbendazim standard liquid are more than 1, relative to
1.0mL carbendazim standard liquids, the addition of solution B is 0.1-0.3mL.
2. the carbendazim detection method according to claim 1 based on fluorescence probe, it is characterised in that step (1) is described
The concentration of solution A is 500ppm.
3. the carbendazim detection method according to claim 1 based on fluorescence probe, it is characterised in that step (2) is described
The concentration of aqueous slkali is 1mol/L.
4. the carbendazim detection method according to claim 1 based on fluorescence probe, it is characterised in that step (3) is described
Prepare the concentration gradient of carbendazim that carbendazim standard liquid select be 0.01ppm, 0.05ppm, 0.2ppm, 1ppm, 5ppm,
25ppm、100ppm。
5. the carbendazim detection method according to claim 1 based on fluorescence probe, it is characterised in that step (4) is used
The volume of testing sample solution is equal to the volume of carbendazim standard liquid used in step (3).
6. the carbendazim detection method according to claim 1 based on fluorescence probe, it is characterised in that add in step (4)
Enter and the solution A and solution B of step (3) moderate.
7. the carbendazim detection method based on fluorescence probe according to any one of claim 1-6, it is characterised in that bag
Include following steps：
(1) by fluorescence probe 9,10- phenanthrenequione dmso solutions, it is configured to 500ppm solution A；
(2) 1mol/L sodium hydroxide solutions B is prepared；
(3) prepare carbendazim standard liquid, the concentration gradient of the carbendazim select is 0.01ppm, 0.05ppm, 0.2ppm,
1ppm, 5ppm, 25ppm, 100ppm, 1.0mL carbendazim standard liquids are taken, add 0.25mL solution As and 0.3mL solution thereto
B, fluorescence signal intensity is detected, draw the fluorescence intensity of carbendazim standard liquid and the standard curve of concentration relationship；
(4) testing sample solution is prepared, takes 1.0mL testing sample solutions, adds 0.25mL solution As and 0.3mL solution thereto
B, fluorescence signal intensity is detected, the standard curve obtained according to step (3) obtains the content of carbendazim in sample.
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| CN110567924B (en) * | 2019-09-02 | 2021-06-08 | 江南大学 | Preparation method of graphene-rare earth composite material and application of graphene-rare earth composite material in benzimidazole pesticide residue combined toxicity effect |
| CN113912650B (en) * | 2021-10-19 | 2023-08-25 | 苏州科技大学 | Fluorescent probe, method, composition and detection product for detecting carbendazim residues |
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