CN104562822A - Application of xylanase stabilizer to papermaking process - Google Patents

Application of xylanase stabilizer to papermaking process Download PDF

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Publication number
CN104562822A
CN104562822A CN201310483863.4A CN201310483863A CN104562822A CN 104562822 A CN104562822 A CN 104562822A CN 201310483863 A CN201310483863 A CN 201310483863A CN 104562822 A CN104562822 A CN 104562822A
Authority
CN
China
Prior art keywords
zytase
ethylhexyl
xylanase
glyceryl ester
phosphate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201310483863.4A
Other languages
Chinese (zh)
Inventor
朱道辰
陈焱
洪好
朱淑静
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ZHENJIANG BIO-INNOVA BIOTECHNOLOGY Co Ltd
Original Assignee
ZHENJIANG BIO-INNOVA BIOTECHNOLOGY Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ZHENJIANG BIO-INNOVA BIOTECHNOLOGY Co Ltd filed Critical ZHENJIANG BIO-INNOVA BIOTECHNOLOGY Co Ltd
Priority to CN201310483863.4A priority Critical patent/CN104562822A/en
Publication of CN104562822A publication Critical patent/CN104562822A/en
Pending legal-status Critical Current

Links

Classifications

    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21C—PRODUCTION OF CELLULOSE BY REMOVING NON-CELLULOSE SUBSTANCES FROM CELLULOSE-CONTAINING MATERIALS; REGENERATION OF PULPING LIQUORS; APPARATUS THEREFOR
    • D21C9/00—After-treatment of cellulose pulp, e.g. of wood pulp, or cotton linters ; Treatment of dilute or dewatered pulp or process improvement taking place after obtaining the raw cellulosic material and not provided for elsewhere
    • D21C9/02—Washing ; Displacing cooking or pulp-treating liquors contained in the pulp by fluids, e.g. wash water or other pulp-treating agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21H—PULP COMPOSITIONS; PREPARATION THEREOF NOT COVERED BY SUBCLASSES D21C OR D21D; IMPREGNATING OR COATING OF PAPER; TREATMENT OF FINISHED PAPER NOT COVERED BY CLASS B31 OR SUBCLASS D21G; PAPER NOT OTHERWISE PROVIDED FOR
    • D21H17/00—Non-fibrous material added to the pulp, characterised by its constitution; Paper-impregnating material characterised by its constitution
    • D21H17/005—Microorganisms or enzymes

Abstract

The invention aims to disclose a xylanase stabilizer which mainly comprises diglycerol phosphate. The diglycerol phosphate is added to and mixed with xylanase, and then the mixture is added to straw pulp for pulp washing, so that the tolerance degree of the xylanase to the high temperature is increased, and the xylanase can react more stably.

Description

The application of a kind of zytase stablizer in paper technology
Technical field
The present invention relates to the stablizer of zytase in a kind of paper technology, especially a kind of di(2-ethylhexyl)phosphate glyceryl ester stablizer.
Background technology
In China, compared with wood pulp papermaking, papermaking straw pulp account for major part.But straw pulp becomes paper fragility large, and intensity difference, this is because xylan content is very high in straw pulp, it is lower than Mierocrystalline cellulose (main component of the paper pulp) polymerization degree, easy aquation.All zytase can be added in present paper technology.Therefore, producing heat resistant xylanase and modifying transformation xylanase gene to improve the stable thermotolerance of xylan is hot topic research aspect in recent years.
Di(2-ethylhexyl)phosphate glyceryl ester (diglycerol phosphate, DP) is a kind of solute of thermophile bacteria body accumulation, and main discovery is present in thermophile bacteria and the ancient bacterium of hyperthermophilic.Its Main Function is the function with biomacromolecules such as protected protein, nucleic acid, cytolemma, enzymes under high temperature, thus reaches the infringement of assisting thermophile bacteria to resist external high temperature.
Summary of the invention
The invention provides a kind of zytase stablizer safe and effective, easy to use.
Realizing of the present invention is a kind of di(2-ethylhexyl)phosphate glyceryl ester stablizer, adds the mixing of di(2-ethylhexyl)phosphate glyceryl ester solution, during pulp washing operation, added in straw pulp by mixture simultaneously in zytase.
The content of described di(2-ethylhexyl)phosphate glyceryl ester solution is 1%-10%.
In zytase, add di(2-ethylhexyl)phosphate glyceryl ester, improve the tolerance degree of zytase to high temperature, thus improve the stability of zytase decomposition xylan.
Zytase stablizer of the present invention has following functions: (1) adds di(2-ethylhexyl)phosphate glyceryl ester in zytase, and adds together when pulp washing operation, improve the stability of xylan, and cost is low.(2) di(2-ethylhexyl)phosphate glyceryl ester use safety.
Embodiment
The present invention is described in detail by following examples, but is not limited to following embodiment.
1. configure di(2-ethylhexyl)phosphate glyceryl ester solution: take the commercially available di(2-ethylhexyl)phosphate glyceryl ester of 10-30g, be settled to 1L.
2. in zytase, add di(2-ethylhexyl)phosphate glyceryl ester, the content of di(2-ethylhexyl)phosphate glyceryl ester solution is 1%-10%.
3., in paper technology, after the physical treatment, boiling of pulping raw material, add zytase, di(2-ethylhexyl)phosphate glyceride mixture at pulp washing, then carry out bleachery.
By adding stablizer di(2-ethylhexyl)phosphate glyceryl ester in zytase, improve the tolerance degree of zytase after straw pulp boiling in high temperature, making the reaction that zytase is more stable.

Claims (3)

1. the stablizer of zytase in paper technology, is characterized in that first being added by di(2-ethylhexyl)phosphate glyceryl ester solution in zytase and adds in straw pulp when pulp washing after mixing.
2. di(2-ethylhexyl)phosphate glyceryl ester solution is water-soluble formulated by mannose group R-Glyceric acid according to claim 1, and concentration is 10-30g/L.
3. the content of di(2-ethylhexyl)phosphate glyceryl ester solution is 1%-10% in zytase, mannose group R-Glyceric acid mixed solution according to claim 1.
CN201310483863.4A 2013-10-16 2013-10-16 Application of xylanase stabilizer to papermaking process Pending CN104562822A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310483863.4A CN104562822A (en) 2013-10-16 2013-10-16 Application of xylanase stabilizer to papermaking process

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310483863.4A CN104562822A (en) 2013-10-16 2013-10-16 Application of xylanase stabilizer to papermaking process

Publications (1)

Publication Number Publication Date
CN104562822A true CN104562822A (en) 2015-04-29

Family

ID=53079839

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310483863.4A Pending CN104562822A (en) 2013-10-16 2013-10-16 Application of xylanase stabilizer to papermaking process

Country Status (1)

Country Link
CN (1) CN104562822A (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1126784A (en) * 1995-12-11 1996-07-17 山东大学 Straw pulp performance improving technology using xylanase
CN1984985A (en) * 2004-04-30 2007-06-20 汉高两合股份公司 Method for production of solid granulates with improved storage stability and abrasion resistance

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1126784A (en) * 1995-12-11 1996-07-17 山东大学 Straw pulp performance improving technology using xylanase
CN1984985A (en) * 2004-04-30 2007-06-20 汉高两合股份公司 Method for production of solid granulates with improved storage stability and abrasion resistance

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PB01 Publication
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Application publication date: 20150429