CN104560975A - Soybean flowering date QTL chromosome mapping interval as well as obtaining method and application thereof - Google Patents
Soybean flowering date QTL chromosome mapping interval as well as obtaining method and application thereof Download PDFInfo
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- CN104560975A CN104560975A CN201510001531.7A CN201510001531A CN104560975A CN 104560975 A CN104560975 A CN 104560975A CN 201510001531 A CN201510001531 A CN 201510001531A CN 104560975 A CN104560975 A CN 104560975A
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Abstract
The invention relates to a soybean flowering date QTL chromosome mapping interval and an obtaining method and application thereof. The soybean flowering date QTL chromosome mapping interval is BAR_04_0012-LJ_SSR-PSI0526. The obtaining method comprises the following steps: first, hybridizing soybean varieties BR121 and Harosory, selfing the seeds obtained from F1 hybrids, and obtaining a recombinant inbred line population; second, determining the genetic distance by Map Manager program QTXb20, and drawing a linkage group map by Mapchart2.1; third, performing genetic mapping on the soybean flowering date with LOD larger than or equal to 2.5 by using MapQTL5.0, wherein the soybean flowering date SSR marked LJ_SSR is taken as a molecular marker of the soybean flowering date to be applied to the soybean genetic breeding.
Description
Technical field
The invention belongs to soybean molecular breeding field, relate to the interval and preparation method of a kind of QTL chromosome mapping and application particularly.
Background technology
Soybean provides important plant protein and oil content for the mankind.Worldwide, northern Northern Europe Sweden to high latitude and North America Canada, reach in the south in the extensive region such as Brazil and Argentina and all have soybean culture, but the latitude span of single kind or the general suitable planting of germ plasm resource is less, gene breeding time of this Regional suitability and soybean is closely related.10 major effect soybean blooms and ripening stage (breeding time) gene locus E1-E9 and J are found so far.But the flowering period of soybean is a quantitative character, controls by multiple gene simultaneously, excavate the quantitative trait locus relevant with soybean growth period, significant for the research enriching soybean growth period regulated and control network further.
Summary of the invention
The object of the present invention is to provide the interval and preparation method of the chromosome mapping of a kind of soybean bloom QTL and application, described mapping interval is No. 4 chromosome mapping interval B AR_04_0012-LJ_SSR-PSI0526 of soybean bloom QTL as shown in table 1, and the Satt519 in this interval can be used as the molecule marker selecting soybean bloom.
The molecule marker in the chromosome mapping interval of the QTL of a kind of soybean bloom of the present invention, the LJ_SSR mapping interval of described soybean bloom is BAR_04_0012-LJ_SSR-PSI0526.
Soybean bloom SSR marker LJ_SSR of the present invention is as the application of molecule marker in soybean heredity breeding selecting soybean bloom.
The preparation method in the chromosome mapping interval of the QTL of a kind of soybean bloom of the present invention, the QTL mapping interval of described soybean bloom is BAR_04_0012-LJ_SSR-PSI0526t_026, and it carries out according to following steps:
One, soybean varieties BR121 and Harosory is hybridized, to F
1the seed that generation obtains carries out selfing, obtains recombinant inbred lines;
Two, utilize Map Manager program QTXb20 to determine genetic distance, draw linkage group collection of illustrative plates with Mapchart 2.1;
Three, utilize MapQTL 5.0, with LOD >=2.5 for standard, genetic mapping is carried out to soybean bloom.
The present invention utilizes soybean varieties BR121 and Harosory to hybridize, and select seed to continue plantation in 127 offsprings of generation, inbreeding of more generation obtains a recombinant inbred lines be made up of 127 strains.These 127 F2 are utilized to construct No. 4 chromosomal genetic maps for individuality.
The present invention utilizes many Interval Mappings of MapQTL 5.0 mapping software, with LOD >=2.5 for standard, carries out genetic mapping to soybean bloom after utilizing Map Manager program QTXb20 to determine genetic distance.No. 4 karyomit(e)s find QTL interval B AR_04_0012-LJ_SSR-PSI0526, as shown in table 1.
The present invention comprises following beneficial effect:
The present invention is the ideal mark interval (being shown in Table 1) of soybean bloom with the interval of LJ_SSR BAR_04_0012-LJ_SSR-PSI0526t_026 placed in the middle, and wherein LJ_SSR is LOD vertex, and its contribution rate is 34.7%.The additive effect of the QTL that mark is closely connected is negative value therewith, and namely BR121 is the donor of this QTL.Therefore, be no matter the location from QTL, or QTL being to the contribution rate of phenotype, is all that the ideal mark of soybean bloom is interval with the interval of LJ_SSR BAR_04_0012-LJ_SSR-PSI0526 placed in the middle.Utilizing the significance of soybean bloom chromosome mapping interval B AR_04_0012-LJ_SSR-PSI0526 as shown in table 1 provided by the invention to be, providing a kind of most economical effective molecular breeding new way for enriching soybean bloom regulated and control network further.
Accompanying drawing explanation
Fig. 1 provides the genetic mapping of soybean bloom of the present invention.
Embodiment
Embodiment one: the chromosome mapping of a kind of soybean bloom QTL is interval, the QTL mapping interval of described soybean bloom is BAR_04_0012-LJ_SSR-PSI0526.
Embodiment two: present embodiment and embodiment one unlike: to map interval B AR_04_0012-LJ_SSR-PSI0526 using LJ_SSR as the QTL of Marker-assisted selection soybean bloom.Other is identical with embodiment one.
Embodiment three: present embodiment and embodiment one or two unlike: described molecule marker LJ_SSR is using the genomic dna of material to be identified as template, carries out the fragment of pcr amplification gained with primer; Described primer sequence is:
Upstream primer: 5 ' GGGAAAAAACTCACTCCTACA 3 '
Downstream primer: 5 ' ATGACAATCTTGAAACTCCCG 3 '.
Other is identical with embodiment one or two.
Embodiment four: one of present embodiment and embodiment one to three unlike: described PCR reaction conditions is as follows:
Pcr amplification condition is: 94 DEG C of denaturation 5min, 94 DEG C of sex change 30s, and 48 DEG C of annealing 30s, 72 DEG C extend 30s, totally 35 circulations, then 72 DEG C extend 10min, 4 DEG C of insulations.Other is identical with one of embodiment one to three.
Embodiment five: one of present embodiment and embodiment one to four unlike: the primer for pcr amplification BAR_04_0012 is:
Upstream primer: 5 ' TTCACACGACAATGTTTGACA 3 '
Downstream primer: 5 ' TCTTGATCTCCCTTGTTTCACA 3 '.
Other is identical with one of embodiment one to four.
Embodiment six: one of present embodiment and embodiment one to five unlike: the primer for pcr amplification PSI0526 is:
Upstream primer: 5 ' TTTTGGAGTTAGGTTTGGTG 3 '
Downstream primer: 5 ' CCATCGCATTTCAAAAGTTA 3 '.
Other is identical with one of embodiment one to five.
Embodiment seven: the soybean bloom SSR marker LJ_SSR of present embodiment is as the application of molecule marker in soybean heredity breeding selecting soybean bloom.
Embodiment eight: present embodiment and embodiment seven unlike: soybean bloom SSR marker LJ_SSR derives from soybean varieties Harosory.Other is identical with embodiment seven.
Embodiment nine: the preparation method in the QTL mapping interval of a kind of soybean bloom of present embodiment, it carries out according to following steps:
One, soybean varieties BR121 and Harosory is hybridized, to F
1the seed that generation obtains carries out selfing, obtains recombinant inbred lines;
Two, utilize Map Manager program QTXb20 to determine genetic distance, draw linkage group collection of illustrative plates with Mapchart 2.1;
Three, utilize MapQTL 5.0, with LOD >=2.5 for standard, genetic mapping is carried out to soybean bloom.
Embodiment ten: present embodiment and embodiment nine unlike: to F in step one
1the seed that generation obtains carries out selfing and obtains F2 for hybrid Population, and utilizes this informative population soybean No. 4 chromosomal genetic linkage mapses.Other is identical with embodiment nine.
Beneficial effect of the present invention is verified by following examples:
Embodiment 1
The foundation of the chromosome mapping interval B AR_04_0012-LJ_SSR-PSI0526t_026 of soybean bloom QTL:
Utilize two flowering period obvious difference soybean varieties BR121 and Harosory configure cross combination, obtain 127 filial generations, utilize these 127 F2 to construct No. 4 chromosomal genetic maps for individuality.
Its PCR experiment schedule of operation is 94 DEG C of sex change 5 minutes, 35 amplification cycles (94 DEG C 30 seconds, 48 DEG C 30 seconds, 72 DEG C 30 seconds), 72 DEG C extend 5 minutes.Pcr amplification product directly detects with the polyacrylamide gel electrophoresis of 6%.Utilize many Interval Mappings of MapQTL 5.0 mapping software, with LOD >=2.5 for standard, genetic mapping is carried out to soybean bloom.And draw linkage group collection of illustrative plates with Map Manager program QTXb20/Mapchart 2.1.
This genetic map has 17 marks, and the molecule marker in collection of illustrative plates comprises 13 SSR marker, 3 promotor specific markers.No. 4 karyomit(e)s find QTL interval B AR_04_0012-LJ_SSR-PSI0526, as shown in table 1.
Wherein, the primer that the whole chromosomal each marker site shown in Fig. 1 carries out PCR mark is as follows:
BAR_04_0001：
Upstream primer: 5 ' TGCAAATTCCAACGGTTTTT 3 '
Downstream primer: 5 ' AACACTGGTTTCACCTTGGC 3 '
BAR_04_0012
Upstream primer: 5 ' TTCACACGACAATGTTTGACA 3 '
Downstream primer: 5 ' TCTTGATCTCCCTTGTTTCACA 3 '
LJ-SSR
Upstream primer: 5 ' GGGAAAAAACTCACTCCTACA 3 '
Downstream primer: 5 ' ATGACAATCTTGAAACTCCCG 3 '
PSI0526
Upstream primer: 5 ' TTTTGGAGTTAGGTTTGGTG 3 '
Downstream primer: 5 ' CCATCGCATTTCAAAAGTTA 3 '
BAR_04_0366
Upstream primer: 5 ' CTCCCCCACCATCTCAATTA 3 '
Downstream primer: 5 ' AAGGTGTTGATCCAGCTCGT 3 '
PSI0532
Upstream primer: 5 ' CAATTTCTGAGTAATGCGGT 3 '
Downstream primer: 5 ' GTTGAGCTTGATGAGATGCT 3 '
Satt646
Upstream primer: 5 ' GCGGGGTATGAATTAATTAATGTAGAAT 3 '
Downstream primer: 5 ' GCGCCTTCAAAAACTAATGACATATCAT 3 '
Satt718
Upstream primer: 5 ' GCGTGCAACACCTCAAGTTTCAAATAC 3 '
Downstream primer: 5 ' GCGTAGCTCTTTCCAAAGTTTTCATC 3 '
AW277661
Upstream primer: 5 ' GCGCATGGAGCATCATCTTCATA 3 '
Downstream primer: 5 ' GCGAGAAAACCCAATCTTTATATCAATA 3 '
Satt339
Upstream primer: 5 ' TAATATGCTTTAAGTGGTGTGGTTATG 3 '
Downstream primer: 5 ' GTTAAGCAGTTCCTCTCATCACG 3 '
Sat_085
Upstream primer: 5 ' GGTTTTAGATCCTTAAATTTGT 3 '
Downstream primer: 5 ' GGGGAAGCAAGTAGCT 3 '
Sat_077
Upstream primer: 5 ' GACACTTGTGGAATTACTCA 3 '
Downstream primer: 5 ' GGGTTGAAGACTTAAATTTGAAATCTCT 3 '
Satt173
Upstream primer: 5 ' TGCGCCATTTATTCTTCA 3 '
Downstream primer: 5 ' AAGCGAAATCACCTCCTCT 3 '
Sat_311
Upstream primer: 5 ' GCGAACACAGAATGACCCTGATTGTAAT 3 '
Downstream primer: 5 ' GCGTGACTCCCACGTTAAAATCTCAAAA 3 '
PSI0582
Upstream primer: 5 ' AAGAAAGTTCTATTTACAAGATCAA 3 '
Downstream primer: 5 ' TACATTTGTATTTCTTGTGTTTTTC 3 '
Satt338
Upstream primer: 5 ' GCGCCCAAGTATTATGAGATATTTGAT 3 '
Downstream primer: 5 ' GCGATAATTTTAAAACTGGACCA 3 '
Satt180
Upstream primer: 5 ' TCGCGTTTGTCAGC 3 '
Downstream primer: 5 ' TTGATTGAAACCCAACTA 3 '
PCR experiment schedule of operation needed for above-mentioned primer is 94 DEG C of sex change 5 minutes, 35 amplification cycles (94 DEG C 30 seconds, 48 DEG C 30 seconds, 72 DEG C 30 seconds), 72 DEG C extend 5 minutes.
The present embodiment finds QTL interval B AR_04_0012-LJ_SSR-PSI0526 on No. 4 karyomit(e)s, as shown in table 1.Wherein LJ_SSR is LOD vertex, and its contribution rate is 34.7%.The additive effect of the QTL that mark is closely connected is negative value therewith, and namely BR121 is the donor of this QTL.Therefore, be no matter the location from QTL, or QTL being to the contribution rate of phenotype, is all that the ideal mark of soybean bloom is interval with the interval of LJ_SSR BAR_04_0012-LJ_SSR-PSI0526 placed in the middle.Utilizing the significance of soybean bloom chromosome mapping interval B AR_04_0012-LJ_SSR-PSI0526 as shown in table 1 provided by the invention to be, providing a kind of most economical effective molecular breeding new way for enriching soybean bloom regulated and control network further.
The QTL of table 1 soybean bloom is interval
The above, be only preferred embodiment of the present invention, is only illustrative for the purpose of the present invention, and nonrestrictive.Those skilled in the art, can carry out multiple change to it in the spirit and scope that the claims in the present invention limit, amendment, and the content of even equivalence replacement, all falls within the scope of protection of the present invention.
Claims (10)
1. the chromosome mapping of a soybean bloom QTL is interval, it is characterized in that the QTL mapping interval of described soybean bloom is BAR_04_0012-LJ_SSR-PSI0526.
2. the chromosome mapping of a kind of soybean bloom QTL according to claim 1 is interval, it is characterized in that the QTL mapping interval B AR_04_0012-LJ_SSR-PSI0526 using LJ_SSR as Marker-assisted selection soybean bloom.
3. the chromosome mapping of a kind of soybean bloom QTL according to claim 1 is interval, it is characterized in that described molecule marker LJ_SSR is using the genomic dna of material to be identified as template, carries out the fragment of pcr amplification gained with primer; Described primer sequence is:
Upstream primer: 5 ' GGGAAAAAACTCACTCCTACA 3 '
Downstream primer: 5 ' ATGACAATCTTGAAACTCCCG 3 '.
4. the chromosome mapping of a kind of soybean bloom QTL according to claim 3 is interval, it is characterized in that:
Described PCR reaction conditions is as follows:
Pcr amplification condition is: 94 DEG C of denaturation 5min, 94 DEG C of sex change 30s, and 48 DEG C of annealing 30s, 72 DEG C extend 30s, totally 35 circulations, then 72 DEG C extend 10min, 4 DEG C of insulations.
5. the chromosome mapping of a kind of soybean bloom QTL according to claim 1 is interval, it is characterized in that the primer for pcr amplification BAR_04_0012 is:
Upstream primer: 5 ' TTCACACGACAATGTTTGACA 3 '
Downstream primer: 5 ' TCTTGATCTCCCTTGTTTCACA 3 '.
6. the chromosome mapping of a kind of soybean bloom QTL according to claim 1 is interval, it is characterized in that the primer for pcr amplification PSI0526 is:
Upstream primer: 5 ' TTTTGGAGTTAGGTTTGGTG 3 '
Downstream primer: 5 ' CCATCGCATTTCAAAAGTTA 3 '.
7. soybean bloom SSR marker LJ_SSR is as the application of molecule marker in soybean heredity breeding selecting soybean bloom.
8. the application of soybean bloom SSR marker LJ_SSR according to claim 7, is characterized in that soybean bloom SSR marker LJ_SSR derives from soybean varieties Harosory.
9. the preparation method in the chromosome mapping interval of soybean bloom QTL, is characterized in that it carries out according to following steps:
One, soybean varieties BR121 and Harosory is hybridized, to F
1the seed that generation obtains carries out selfing, obtains recombinant inbred lines;
Two, utilize Map Manager program QTXb20 to determine genetic distance, draw linkage group collection of illustrative plates with Mapchart 2.1;
Three, utilize MapQTL 5.0, with LOD >=2.5 for standard, genetic mapping is carried out to soybean bloom.
10. the interval preparation method of the chromosome mapping of a kind of soybean bloom QTL according to claim 9, is characterized in that in step one F
1the seed that generation obtains carries out selfing and obtains F2 for hybrid Population, and utilizes this informative population soybean No. 4 chromosomal genetic linkage mapses.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104357442A (en) * | 2014-10-25 | 2015-02-18 | 中国科学院东北地理与农业生态研究所 | QTL mapping region for soybean flowering stage and obtaining method as well as application |
| CN111676308A (en) * | 2020-06-01 | 2020-09-18 | 中国农业科学院作物科学研究所 | QTL (quantitative trait locus) and SNP (Single nucleotide polymorphism) marker related to quantitative traits of soybean branches and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008021413A1 (en) * | 2006-08-15 | 2008-02-21 | Monsanto Technology Llc | Compositions and methods of plant breeding using high density marker information |
| CN103014019A (en) * | 2012-12-04 | 2013-04-03 | 中国科学院东北地理与农业生态研究所 | Flowering gene GmCOL1b of soybean and coding protein thereof |
-
2015
- 2015-01-04 CN CN201510001531.7A patent/CN104560975A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008021413A1 (en) * | 2006-08-15 | 2008-02-21 | Monsanto Technology Llc | Compositions and methods of plant breeding using high density marker information |
| CN103014019A (en) * | 2012-12-04 | 2013-04-03 | 中国科学院东北地理与农业生态研究所 | Flowering gene GmCOL1b of soybean and coding protein thereof |
Non-Patent Citations (6)
| Title |
|---|
| CARLOS A. CAIRO ET AL.: "Tagging the juvenile locus in soybean [Glycine max (L.) Merr.] with molecular markers", 《 EUPHYTICA》 * |
| HAIYANG NAN 等: "GmFT2a and GmFT5a Redundantly and Differentially Regulate Flowering through Interaction with and Upregulation of the bZIP Transcription Factor GmFDL19 in Soybean", 《PLOS ONE》 * |
| KUNIHIKO KOMATSU 等: "Identification of QTL controlling post-flowering period in soybean", 《BREEDING SCIENCE》 * |
| 王涛 等: "两个大豆开花期 QTL 定位及对农艺性状的影响分析", 《华北农学报》 * |
| 王英 等: "开花后不同光周期条件下大豆农艺性状和品质性状的 QTL 分析", 《作物学报》 * |
| 苏成付 等: "利用目标区段剩余杂合系进行大豆开花期 QTL 的验证和精细定位", 《科学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104357442A (en) * | 2014-10-25 | 2015-02-18 | 中国科学院东北地理与农业生态研究所 | QTL mapping region for soybean flowering stage and obtaining method as well as application |
| CN111676308A (en) * | 2020-06-01 | 2020-09-18 | 中国农业科学院作物科学研究所 | QTL (quantitative trait locus) and SNP (Single nucleotide polymorphism) marker related to quantitative traits of soybean branches and application |
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Application publication date: 20150429 |