CN104560939B - A kind of preparation method of bacteria carrier particle - Google Patents
A kind of preparation method of bacteria carrier particle Download PDFInfo
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- CN104560939B CN104560939B CN201410717114.8A CN201410717114A CN104560939B CN 104560939 B CN104560939 B CN 104560939B CN 201410717114 A CN201410717114 A CN 201410717114A CN 104560939 B CN104560939 B CN 104560939B
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- bacterium
- particle
- core
- bacteria
- encapsulating layer
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- 241000894006 Bacteria Species 0.000 title claims abstract description 50
- 239000002245 particle Substances 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000002351 wastewater Substances 0.000 claims abstract description 11
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims abstract description 6
- 239000000853 adhesive Substances 0.000 claims abstract description 6
- 230000001070 adhesive effect Effects 0.000 claims abstract description 6
- 239000011324 bead Substances 0.000 claims abstract description 6
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 6
- 239000000945 filler Substances 0.000 claims abstract description 5
- 239000000565 sealant Substances 0.000 claims abstract description 4
- 230000003321 amplification Effects 0.000 claims abstract description 3
- 239000001110 calcium chloride Substances 0.000 claims abstract description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims abstract description 3
- 238000004132 cross linking Methods 0.000 claims abstract description 3
- 239000001963 growth medium Substances 0.000 claims abstract description 3
- 238000000703 high-speed centrifugation Methods 0.000 claims abstract description 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract description 3
- 229920000915 polyvinyl chloride Polymers 0.000 claims abstract description 3
- 239000004800 polyvinyl chloride Substances 0.000 claims abstract description 3
- 238000007493 shaping process Methods 0.000 claims abstract description 3
- 239000002202 Polyethylene glycol Substances 0.000 claims description 7
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 7
- 229920001223 polyethylene glycol Polymers 0.000 claims description 7
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 6
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 5
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical group [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims description 5
- 235000010413 sodium alginate Nutrition 0.000 claims description 5
- 239000000661 sodium alginate Substances 0.000 claims description 5
- 229940005550 sodium alginate Drugs 0.000 claims description 5
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical group O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000001099 ammonium carbonate Substances 0.000 claims description 4
- 235000012501 ammonium carbonate Nutrition 0.000 claims description 4
- 239000004952 Polyamide Substances 0.000 claims description 2
- 238000012644 addition polymerization Methods 0.000 claims description 2
- 150000001875 compounds Chemical class 0.000 claims description 2
- 230000000593 degrading effect Effects 0.000 claims description 2
- 230000001295 genetical effect Effects 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- 244000005700 microbiome Species 0.000 claims description 2
- 230000000243 photosynthetic effect Effects 0.000 claims description 2
- 229920002647 polyamide Polymers 0.000 claims description 2
- 230000002459 sustained effect Effects 0.000 claims description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 19
- 239000010410 layer Substances 0.000 description 11
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 238000009792 diffusion process Methods 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 2
- 235000013339 cereals Nutrition 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 241000186046 Actinomyces Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 238000005842 biochemical reaction Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000012792 core layer Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 235000013379 molasses Nutrition 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000010802 sludge Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- MEYZYGMYMLNUHJ-UHFFFAOYSA-N tunicamycin Natural products CC(C)CCCCCCCCCC=CC(=O)NC1C(O)C(O)C(CC(O)C2OC(C(O)C2O)N3C=CC(=O)NC3=O)OC1OC4OC(CO)C(O)C(O)C4NC(=O)C MEYZYGMYMLNUHJ-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 235000020985 whole grains Nutrition 0.000 description 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The invention discloses a kind of preparation method of bacteria carrier particle, including：Step 1：Make core A:Core A is the coccoid particle being prepared by mixing into by filler, expanding agent and adhesive, is then dried；Step 2：In core A external applications oxireme, oxirane or polyvinyl chloride as sealant B, turn into an enclosed interior and contain cuniculate bead；Step 3：In suitable culture medium, bacterium is cultivated by progressively amplification, to the 5th generation, is concentrated with high speed centrifugation, controls 0~4 DEG C of temperature, mixed with encapsulating layer C；Step 4：The encapsulating layer C of mixing is coated in bead surface, forms the outer layer of bacterium embedded particles；Step 5：Under 0~4 DEG C of temperature conditions, the particle is squeezed into CaCl2The immobilization particle of crosslinking shaping about 4~6mm diameters in solution.The beneficial effects of the invention are as follows：It is easy to cultivate bacterium in wastewater disposal basin, particle is capable of the bacterium of high capacity.
Description
Technical field
The invention belongs to the preparation method of carrier granular, is related to a kind of preparation method of bacteria carrier particle.
Background technology
Immobilization technology refers to enzyme immobilizatio and the immobilization technology of bacterium.Since the seventies in last century, people are first
First exploitation has used enzyme immobilization technology, and the immobilization technology of bacterium is grown up on the basis of enzyme immobilization technology
's.Immobilization bacterium technology is exactly using chemically or physically Method means, by the bacteria adhension of common free state in a certain limit
Fixed space, with and improve the concentration of bacterium, and take certain method bacterium is kept higher activity, extend using period,
It can accomplish to recycle.Research and application show：By immobilization bacterium technology bacterial density raising, biochemical process can be made anti-
Speed is answered to accelerate, the resistance to murder by poisoning ability of bacterium is strong, bacterium is lost in less during successive reaction or continuous degradation, raw material and product divide
The advantages that being minimized from easy, stable, reaction unit.At present, immobilization bacterium technology is to good performance, extensively
The general research applied in terms of environmental pollution improvement, main object of administering is intractable organic wastewater and heavy metal pollution
Waste water, while achievement is related to the pollution control of air and soil.
Although bacteria adhension has above advantage, there is also light diffusion, light transmission, substrate and product in reaction solution
Diffusion mass transfer is difficult, the problems such as immobilization particle easy dispersing and dissolving.At present, surface particles are all integration, whole grain.This
Patent devises a kind of embedded particles new technology first, and embedded particles are divided into the core layer of centre and outside encapsulating layer.By
It is poor in existing particle internal mass transfer, transmitted light is not easy, has extraneous (solution) to be not easy to carry out mass exchange, and bacterium inside particle
It is difficult to enter, bacterial growth is few, therefore inner sealing is got up, and is configured as the skeleton of particle, plays floating and fixation；Outside
Layer can fully receive light, facilitate conveying between mass transfer and material.So it is bacterial growth layer, degradation by exterior design
Layer, is embedded in outer layer by bacterium, porosity and channel diameter can be easily increased in outer layer.Solve light diffusion and substrate with this
And product diffusion mass transfer is difficult.Improve the validity of the degraded of embedded particles and improve the influence of glucose substrate degradation characteristic.
The content of the invention
It is an object of the present invention to overcome the deficiencies in the prior art, there is provided one kind is easy to Bacteria Culture, is run in MBR
Cheng Zhongke improve degradation speed, reduce sludge quantity bacteria carrier particle preparation method.
The preparation method of the bacteria carrier particle of the present invention, including：
Step 1：Make core A:Core A is the coccoid particle being prepared by mixing into by filler, expanding agent and adhesive, its
Ratio is 50~80%, 10%~25%, 10~25%；2~3mm diameters, are then dried, and ensure the hardness of particle and certain
Elasticity, internally there is certain space；The filler is diatomite, polyamide or activated carbon；The expanding agent is ammonium carbonate；Institute
It is addition polymerization ethylene glycol, polyvinyl alcohol or oxirane to state adhesive；
Step 2：In core A external applications oxireme, oxirane or polyvinyl chloride as sealant B, turn into one
Enclosed interior contains cuniculate bead；
Step 3：In suitable culture medium, bacterium is cultivated by progressively amplification, it is dense with high speed centrifugation to the 5th generation
Contracting, 0~4 DEG C of temperature is controlled, mixed with encapsulating layer C；The encapsulating layer C is by sodium alginate, polyethylene glycol, polyvinyl alcohol system
, its ratio is 70%~90%, 5%~15%, 5%~15%, has stronger cohesive to have preferable elasticity, and can be useless
Bacterium is protected in water, is progressively sustained bacterium；
Step 4：The encapsulating layer C of mixing is coated in bead surface, forms the outer layer of bacterium embedded particles；
Step 5：Under 0~4 DEG C of temperature conditions, the particle is squeezed into CaCl2Crosslinking shaping 4~6mm diameters in solution
Immobilization particle.
As preferred：The bacterium of embedding is actinomyces, photosynthetic bacteria, Degrading genetical engineering of microorganism or production compound bacteria.
The beneficial effects of the invention are as follows：
1st, the bacteria carrier particle of the invention as made from a kind of bacteria adhension technology, it is thin to be easy to culture in wastewater disposal basin
Bacterium, the particle can reach efficient, impact-resistant effect, simultaneously because the containing of sodium alginate, polyethylene glycol, polyvinyl alcohol
Property so that particle is capable of the bacterium of high capacity.
2nd, the particle is easy to use in common biochemical reaction tank, can also be coupled with membrane bioreactor.
3rd, it is not easily broken, especially internal core has tunic protection so that it is internal intact all the time, while can protect
Grain has floatability.
4th, the cavity size of internal core, how much can be adjusted by the quantity of ammonium carbonate, control, realize regulation particle
Proportion, it is possible thereby to position of the embedded particles in water be adjusted, such as at waste liquid pool bottom or top, or in centre floating.
5th, carrier granular of the invention can load 1:1~l:5 ratio embedding bacterium (bacterial weight：Embedding medium weight
Amount).The particle squeezes into CaCl under 0~4 DEG C of temperature conditions, by little particle2, be crosslinked in the solution such as glutaraldehyde, being fixed
Particle.
Brief description of the drawings
Fig. 1 is schematic diagram of the present invention；
Embodiment
The present invention is described further with reference to the accompanying drawings and examples.Although the present invention will enter with reference to preferred embodiment
Row description, it should be understood that being not offered as limiting the invention in the embodiment.It can be included on the contrary, the present invention will cover
Alternative, modified and the equivalent in the scope of the present invention that appended claims limits.
As shown in figure 1, the preparation method of the bacteria carrier particle of the present invention：Grain skeleton is formed by diatomite, with carbonic acid
Ammonium is expanding agent, prepares to form 20~30mm granular cores using polyethylene glycol as adhesive, diatomite, polyethylene glycol, ammonium carbonate
The ratio between be 7:2:1, the thick embryo of the core through drying, 80 DEG C roasting etc. handling process, inside be to have certain cavity.Use oxirane
As sealant.By the use of sodium alginate, polyethylene glycol, polyvinyl alcohol as encapsulating layer embed bacterium, sodium alginate, polyethylene glycol,
The ratio between polyvinyl alcohol is 5:2:1,40~60mm immobilization particle is formed, particle can freely move in water made from the technology
It is dynamic.The activity and impact resistance of bacterium can be improved.There are some successful cases using bacteria adhension technology：
Case study on implementation 1
It is 3000mg/L, COD 1500mg/ in dyeing waste water BOD using a certain amount of carrier granular as made from the technology
In L solution, carrier granular injected volume is 1~2.5%, under aerobic condition, is degraded through 3 days, BOD have dropped 81.5%, COD
It has dropped 83.0%.
Case study on implementation 2
It is the molasses that 5000mg/L, COD are 1800mg/L in BOD using a certain amount of carrier granular as made from the technology
In waste water, carrier granular injected volume is 0.5~1.5%, under aerobic condition, is degraded through 3 days, BOD have dropped 90.5%, COD
It has dropped 93.6%.
Case study on implementation 3
It is the soya-bean milk that 5500mg/L, COD are 2200mg/L in BOD using a certain amount of carrier granular as made from the technology
In waste water, carrier granular injected volume is 0.5~5%, under aerobic condition, is degraded through 3 days, BOD have dropped under 95.5%, COD
Drop 92.3%.
Case study on implementation 4
It is the life that 2500mg/L, COD are 2400mg/L in BOD using a certain amount of carrier granular as made from the technology
In waste water, carrier granular injected volume is 1~3.4%, under anaerobic, is degraded through 3 days, under aerobic condition, is dropped through 2 days
Solution, BOD have dropped 94.5%, COD and have dropped 94.6%.
Case study on implementation 5
It is the rubbish that 8500mg/L, COD are 5500mg/L in BOD using a certain amount of carrier granular as made from the technology
Effusion, carrier granular injected volume is 0.5~2.5%, under anaerobism, aerobic condition, is respectively degraded through 5 days, BOD have dropped
85.5%th, COD have dropped 79.1%.
Case study on implementation 6
It is the pharmacy that 3500mg/L, COD are 1800mg/L in BOD using a certain amount of carrier granular as made from the technology
In waste water, ammonia nitrogen waste water 180mg/L, carrier granular injected volume is 1~3.5%, under anaerobism, aerobic condition, is respectively dropped through 5 days
Solution, BOD have dropped 90.6%, COD and have dropped 81.8%, and ammonia nitrogen declines 86.1%.
Claims (2)
- A kind of 1. preparation method of bacteria carrier particle, it is characterised in that including：Step 1：Make core A:Core A is the coccoid particle being prepared by mixing into by filler, expanding agent and adhesive, its ratio For 50~80%, 10%~25%, 10~25%；2~3mm diameters, are then dried, and ensure the hardness of particle and certain bullet Property, internally there is certain space；The filler is diatomite, polyamide or activated carbon；The expanding agent is ammonium carbonate；It is described Adhesive is addition polymerization ethylene glycol, polyvinyl alcohol or oxirane；Step 2：In core A external applications oxireme, oxirane or polyvinyl chloride as sealant B, turn into one it is closed Inside contains cuniculate bead；Step 3：In suitable culture medium, bacterium is cultivated by progressively amplification, to the 5th generation, is concentrated, controlled with high speed centrifugation 0~4 DEG C of temperature processed, mixed with encapsulating layer C；The encapsulating layer C is made by sodium alginate, polyethylene glycol, polyvinyl alcohol, its Ratio is 70%~90%, 5%~15%, 5%~15%, has stronger cohesive to have preferable elasticity, and can be in waste water Bacterium is protected, is progressively sustained bacterium；Step 4：The encapsulating layer C of mixing is coated in bead surface, forms the outer layer of bacterium embedded particles；Step 5：Under 0~4 DEG C of temperature conditions, the particle is squeezed into CaCl2The immobilization of crosslinking shaping 4~6mm diameters in solution Particle.
- 2. the preparation method of bacteria carrier particle according to claim 1, it is characterised in that：The bacterium of embedding is unwrapping wire Bacterium, photosynthetic bacteria, Degrading genetical engineering of microorganism or production compound bacteria.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410717114.8A CN104560939B (en) | 2014-12-01 | 2014-12-01 | A kind of preparation method of bacteria carrier particle |
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| CN201410717114.8A CN104560939B (en) | 2014-12-01 | 2014-12-01 | A kind of preparation method of bacteria carrier particle |
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| Publication Number | Publication Date |
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| CN104560939A CN104560939A (en) | 2015-04-29 |
| CN104560939B true CN104560939B (en) | 2018-03-13 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039300A (en) * | 2015-08-19 | 2015-11-11 | 浙江工业大学 | Preparation method of heterogeneous bacteria embedding particles |
| CN111333200B (en) * | 2020-03-18 | 2022-07-05 | 运城学院 | Embedded immobilized microorganism particles, preparation method and sewage treatment method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101544970A (en) * | 2009-05-08 | 2009-09-30 | 周鑫 | Immobilized carrier of core-shell composite structure and its preparing process |
| CN101768583A (en) * | 2010-01-22 | 2010-07-07 | 中国海洋大学 | Method for immobilizing oil degradation bacteria with reed straw as carrier and application thereof |
| CN101875928A (en) * | 2009-04-29 | 2010-11-03 | 中国环境科学研究院 | Embedding immobilization method for microbial preparation |
| CN103212449A (en) * | 2013-04-15 | 2013-07-24 | 沈阳三聚凯特催化剂有限公司 | Hydrofining catalyst carrier and preparation method thereof as well as hydrofining catalyst using carrier and preparation method of hydrofining catalyst |
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2014
- 2014-12-01 CN CN201410717114.8A patent/CN104560939B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101875928A (en) * | 2009-04-29 | 2010-11-03 | 中国环境科学研究院 | Embedding immobilization method for microbial preparation |
| CN101544970A (en) * | 2009-05-08 | 2009-09-30 | 周鑫 | Immobilized carrier of core-shell composite structure and its preparing process |
| CN101768583A (en) * | 2010-01-22 | 2010-07-07 | 中国海洋大学 | Method for immobilizing oil degradation bacteria with reed straw as carrier and application thereof |
| CN103212449A (en) * | 2013-04-15 | 2013-07-24 | 沈阳三聚凯特催化剂有限公司 | Hydrofining catalyst carrier and preparation method thereof as well as hydrofining catalyst using carrier and preparation method of hydrofining catalyst |
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| CN104560939A (en) | 2015-04-29 |
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