CN104560805A - Method for preparing cell protein through fermentation of liquid waste in ginkgo leaf extract production - Google Patents
Method for preparing cell protein through fermentation of liquid waste in ginkgo leaf extract production Download PDFInfo
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Abstract
The invention discloses a method for preparing cell protein through fermentation of liquid waste in ginkgo leaf extract production. The method comprises the following steps: putting the liquid waste adsorbed by a macroporous adsorption resin in the ginkgo leaf extract production into a fermentation tank, heating to be boiling for 2 hours, cooling to 25-30 DEG C, regulating pH to 4.8-5.2, then heating to be boiling for 1 hour for sterilizing, cooling to 36-38 DEG C, inoculating a composite microbial strain fermenting agent which is 15% of the weight of a culture solution, stirring for culturing for 18-22 hours at 36-38 DEG C and at a ventilation amount of 1: 0.6V/ V/ min, centrifugating for removing water and collecting a solid matter in a baking pan after the end of fermentation culture, and performing vacuum drying at 55 DEG C till the moisture content is less than 7% to obtain the cell protein product. In the method, the liquid waste adsorbed by the macroporous adsorption resin during decoction of ginkgo leaves for extracting ginkgo flavone is used; the method is suitable for industrial centralized processing; the utilization ratio of the liquid waste is high; in the preparation process, concentration is not required, so that energy consumption is low, the cost is low, economic and environmental benefits are high, and the implementation prospect is good; the cell protein is a scarce protein raw material at present, and has a very good market prospect.
Description
Technical field
The present invention relates to wasted resources, processing of farm products field, the waste liquid produced in being specifically related to utilize Folium Ginkgo extract to produce is through the method for fermentation for cell protein.
Background technology
Folium Ginkgo extract is by Ginkgo Leaf through pulverizing, with alcohol heating reflux extraction, and united extraction liquid, reclaim ethanol and be concentrated into appropriate, being added on processed good macroporous adsorptive resins, using the ethanol elution of water and different concns successively, collect corresponding elutriant, reclaim ethanol, spraying dry; Or recovery ethanol, be condensed into thick paste, vacuum-drying, pulverize, obtained (Chinese Pharmacopoeia Commission's Pharmacopoeia of People's Republic of China, version in 2010, one, 392 pages).But, the manufacturer of existing Folium Ginkgo extract is all optimized the extraction process of Ginkgo Leaf, its technique many employings dry Folium Ginkgo is direct or pulverize rear boiling, extract, united extraction liquid, be added in processed good macroporous resin column, adsorb, then carry out wash-out with the ethanol of water and different concns successively.No matter adopt method technique or the Optimization Technology of enterprise of " pharmacopeia ", the extracted amount of ginkgo biloba leaf total brass extract is only at 0.2-0.425%(dry product meter) between, " pharmacopeia " is although define Ginkgo Leaf and calculate by dry product, must not 0.40% be less than containing total flavonoids, must not 0.25% be less than containing terpene lactone, but it is different with regard to the collecting season of Ginkgo Leaf, the difference of growing environment difference and processing and storage condition, the content difference of its bilobanone is also larger, but the extracted amount being no matter extract is much, and its maximum level extracted amount is all below 0.50%.But in the processing enterprise of Folium Ginkgo extract, in the process extracting flavonol glycosides, which enterprise of family a large amount of extracting solutions, all by as discharging of waste liquid, does not have carry out recovery processing and utilization in the course of processing of its Folium Ginkgo extract to its waste liquid at present.The liquid waste disposal approach that current Ginkgo Leaf extracts processing enterprise has two, one is direct discharge, and to periphery soil, water resources pollutes is undoubtedly, and two is that unit with good conditionsi adopts Sewage treatment systems to carry out processing rear discharge, add economical load, improving production cost is also undoubtedly.
Ginkgo Leaf has very high nutritive value, containing effective constituents such as 46 kinds of flavonoid compounds and terpene, phenols, trace element and amino acid, Ginkgo Leaf Middle nutrition component content is very abundant, in butt, protein 10.9% ~ 15.5% in Ginkgo Leaf, total reducing sugar 7.38% ~ 8.69%, reducing sugar 4.64% ~ 5.63%, vitamins C 66.78 ~ 129.20mg/100g, vitamin-E 6.17 ~ 8.05 mg/100g, vitamins B
10.06 ~ 0.09 mg/100g, vitamins B
20.30 ~ 0.45 mg/100g, carotene 14.52 ~ 18.80 mg/100g, choline 28.00 ~ 39.50 mg/100g.Various aminoacids content: aspartic acid 1.26 ~ 1.73g/100g, Threonine 0.50 ~ 0.72 g/100g, Serine 0.55 ~ 0.72 g/100g, L-glutamic acid 1.16 ~ 1.79 g/100g, glycine 0.7 ~ 0.92 g/100g, L-Ala 0.71 ~ 1.09 g/100g, α-amino-isovaleric acid 0.64 ~ 0.99 g/100g, methionine(Met) 0.18 ~ 0.24 g/100g, Isoleucine 0.44 ~ 0.63 g/100g, leucine 0.83 ~ 1.10 g/100g, tyrosine 0.37 ~ 0.56 g/100g, phenylalanine 0.60 ~ 0.83 g/100g, γ-aminobutyric acid 0.18 ~ 0.34 g/100g, Histidine 0.23 ~ 0.34 g/100g, Methionin 0.80 ~ 1.01 g/100g, tryptophane 0.21 ~ 0.23 g/100g, arginine 0.60 ~ 0.91 g/100g, proline(Pro) 0.69 ~ 1.28 g/100g, total amino acid 10.73 ~ 15.43 g/100g, indispensable amino acid 4.45 ~ 6.04 g/100g, wherein indispensable amino acid/total amino acid 39.14 ~ 41.47%, indispensable amino acid/non-essential amino acid 64.46 ~ 70.86%, various Mineral Elements Content: calcium 1860 ~ 2360mg/100g, phosphorus 298.10 ~ 407.10mg/100g, iron 22.85 ~ 63.56 mg/100g, fluorine 6.00 ~ 13.00 mg/100g, copper 0.56 ~ 0.73 mg/100g, manganese 2.94 ~ 6.10 mg/100g, zinc 1.43 ~ 1.80 mg/100g, chromium <0.12 mg/100g, cobalt <0.12 mg/100g, boron 30.67 ~ 55.54 μ g/100g, selenium 5.45 ~ 15.44 μ g/100g.No matter being all not less than soybean protein, Chicken Albumin and WHO pattern from content with being worth with regard to indispensable amino acid in Ginkgo Leaf (butt) and the indispensable amino acid in quality protein, WHO model comparision (as following table) its Ginkgo Leaf, proving that its Ginkgo Leaf has very high nutritive value.
table: indispensable amino acid and quality protein, WHO model comparision in Ginkgo Leafunit: g/100g (protein)
| Amino acid name | Albumen in ginkgo | Soybean protein | Chicken Albumin | WHO |
| Threonine | 44.5～50.5 | 37.0 | 47.0 | 9.0 |
| α-amino-isovaleric acid | 55.8～64.1 | 48.0 | 66.0 | 13.0 |
| Methionine(Met) | 14.5～17.0 | 11.0 | 57.0 | 17.0 |
| Isoleucine | 36.4～40.8 | 49.0 | 54.0 | 13.0 |
| Leucine | 71.2～76.1 | 77.0 | 86.0 | 19.0 |
| Phenylalanine+tyrosine | 84.1～90.1 | 91.0 | 93.0 | 19.0 |
| Histidine | 21.0～22.0 | 25.0 | 22.0 | 16.0 |
| Methionin | 65.4～73.4 | 61.0 | 70.0 | 16.0 |
| Tryptophane | 13.6～21.1 | 14.0 | 17.0 | 5.0 |
But, in the process that Folium Ginkgo extract (GBE) is produced, the total flavones composition only just extracting 0.2-0.425% is extracted by macroporous resin adsorption, and other organic and inorganic useful nutritive ingredient is all left in the aqueous solution as discharging of waste liquid, this not only can give environment, a lot of beneficiating ingredients with better nutritivity value goes out of use, and causes the significant wastage of resource.
Ginkgo industry is enriched people the mainstay industry in strong county in Jiangsu Pizhou City, the leading whole nation of cultivated area of ginkgo, the resource of Ginkgo Leaf is very abundant, annual results dry Folium Ginkgo reaches more than 4.6 ten thousand tons, the local bilobanone in Pizhou City extracts personal about 3.1 ten thousand tons of processing enterprise, at present, the enterprise of Pizhou City's process for processing Folium Ginkgo extract has 8, consume dry Folium Ginkgo every year with regard to Pizhou Xinyuan Biological Products Co., Ltd. and reach 10,000 tons, Xuzhou Tianli Biological Technology Co., Ltd. consumes dry Folium Ginkgo 0.5 ten thousand tons, bass special biological products company limited in Xuzhou consumes dry Folium Ginkgo 0.6 ten thousand tons, other enterprise's year wastage in bulk or weight dry Folium Ginkgos about 10,000 tons.By the working method of existing Folium Ginkgo extract, general decoction extraction is Ginkgo Leaf (butt) is 1:10-20 with the usage ratio of water, 3.1 ten thousand tons of calculating are consumed year by the whole city of current Pizhou City, the productivity waste liquid that the course of processing extracting flavones with regard to Ginkgo Leaf every year produces just reaches 31-62 ten thousand tons, and the waste liquid that the annual decoction with regard to Ginkgo Leaf as seen, leaching process produce is surprising.This resource is utilized to find the new protein resource of exploitation; then as the nutrition source of animal-feed; existing waste resource can not only be utilized well; make it to turn waste into wealth; more be of value to innocuousness protection of the environment; reducing the discharge of waste liquid, reduce the purifying treatment of waste liquid, develop new cell protein resource and no matter from which angle go, can be all that enterprise and society reduce Financial cost, and creation economic worth.Through retrieval, just yet there are no the report of the waste liquid fermentation in producing with Folium Ginkgo extract (GBE) for cell protein in existing domestic and international patent or document.
Summary of the invention
The process extracting flavones with regard to Ginkgo Leaf processing can be found out from above-mentioned statement, its extract is only the flavone component of 0.2-0.45%, and a large amount of protein, amino acid, carbohydrate, vitamins, mineral element and trace element are all left in the decoction extracting solution of Ginkgo Leaf, these useful compositions are again the good source preparing animal-feed.Waste liquid fermentation in producing with Folium Ginkgo extract (GBE) in process is for microbial cell proteins, abundant in waste liquid after utilizing fermentable to be extracted by Ginkgo Leaf exactly, non-digestible protein transduction becomes that indispensable amino acid composition more balances, the tropina of readily digested absorption, with multiple-microorganism fermentation, degraded amino acid becomes polypeptide or small peptide small-molecule substance, starch and food fibre to be degraded into oligose or monose; During the fermentation, the metabolism of microorganism and breeding, produce the cell protein being beneficial to animal in a large number and absorbing.
Waste liquid fermentation in the object of the invention is to utilize Folium Ginkgo extract (GBE) to produce is for cell protein.
In order to improve the waste liquid ferment effect in Ginkgo Leaf processing, present invention employs the fermentation process of composite bacteria microbial bacteria starter, simultaneously in order to promote the quality and quantity of cell protein, also consider the nutritional need of microbial bacteria in fermentating metabolism process simultaneously, the breeding amount of microbial bacteria is increased, present invention employs different fermentation process, but be consistent with regard to fermentation for the object of cell protein.
To achieve these goals, the present invention is preferably by the following technical solutions:
In utilizing Folium Ginkgo extract to produce, waste liquid fermentation is for the method for cell protein, have employed following processing method respectively:
The first scheme is: the fresh waste liquid flowed out after absorption with macroporous adsorbent resin ginkgolic flavone glycoside in being produced by Folium Ginkgo extract, insert in fermentor tank, heated and boiled sterilizing in 2 hours, in time being cooled to 25-30 DEG C, pH4.8-5.2 is regulated with dilute phosphoric acid (98% strong phosphoric acid 1.5L is diluted with water to 40L), reheat and boil sterilizing in 1 hour, in time being cooled to 36-38 DEG C, obtain nutrient solution, complex microorganism bacterium leavening agent weight 15% is added by nutrient solution weight ratio, under 36-38 DEG C of condition, stir aerated culture fermentation 18-22 hour, after cultivation and fermentation terminates, after fermentation liquor centrifuge dehydration, collect solid and be placed in drip pan, 7% is less than to water content in 55 DEG C of vacuum-dryings, obtain cell protein.
First scheme is: the waste liquid flowed out after absorption with macroporous adsorbent resin ginkgolic flavone glycoside in being produced by Folium Ginkgo extract, insert in fermentor tank, heated and boiled sterilizing in 2 hours, in time being cooled to 25-30 DEG C, add wort weight 5%, ammonium sulfate weight 0.5-1.5%, ferrous sulfate (FeSO by waste liquid weight ratio
4.4H
2o) weight 0.013-0.2%, magnesium sulfate (MgSO
4.7H
2o) weight 0.03-0.05%, calcium chloride (CaCl
2.2H
2o) weight 0.15-0.3%, stir, pH4.8-5.2 is regulated with dilute phosphoric acid (98% strong phosphoric acid 1.5L is diluted with water to 40L), reheat and boil sterilizing in 1 hour, in time being cooled to 36-38 DEG C, obtain nutrient solution, complex microorganism bacterium leavening agent weight 15% is added by nutrient solution weight ratio, under 36-38 DEG C of condition, stir aerated culture fermentation 18-22 hour, after cultivation and fermentation terminates, after fermentation liquor centrifuge dehydration, collect solid and be placed in drip pan, be less than 7% in 55 DEG C of vacuum-dryings to water content, obtain cell protein.
The third scheme is: the fresh waste liquid flowed out after absorption with macroporous adsorbent resin ginkgolic flavone glycoside in being produced by Folium Ginkgo extract, insert in fermentor tank, heated and boiled sterilizing in 2 hours, in time being cooled to 25-30 DEG C, bran powder or the Semen Maydis powder of weight 12-15% is added by waste liquid weight ratio, stir, pH4.8-5.2 is regulated with dilute phosphoric acid (98% strong phosphoric acid 1.5L is diluted with water to 40L), reheat and boil sterilizing in 1 hour, in time being cooled to 36-38 DEG C, complex microorganism bacterium leavening agent weight 15% is added by nutrient solution weight ratio, under 36-38 DEG C of condition, stir aerated culture fermentation 18-22 hour, after cultivation and fermentation terminates, after fermentation liquor centrifuge dehydration, collect solid and be placed in drip pan, 7% is less than to water content in 55 DEG C of vacuum-dryings, obtain cell protein.
Here is further optimization to technique scheme and/or selection.
In above-mentioned one to three kind of scheme, described complex microorganism bacterium leavening agent is that aspergillus niger (Aspergillus niger), genus bacillus (Bacillus) and yeast (Yeast) are mixed in proportion composite forming, and its weight proportion is aspergillus niger: genus bacillus: yeast=1:1:3 ratio.
Described genus bacillus (Bacillus) is subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus licheniformis), Bacillus cereus (Bacillus cereus), Bacillus coagulans (Bacillus coagulans), any one in bacillus pumilus (Bacillus pumilus) and bacillus lentus (Slow bacillus).
Described yeast (Yeast) is candida tropicalis (Candida tropicalis), any one in Candida utilis (Candida utilis), cereuisiae fermentum (Beer yeast) and fodder yeast (Feeding yeast).
Aspergillus niger described above, genus bacillus and yeast can select all dry powder goods being used as foodstuff additive or fodder additives commercially available.
The viable bacteria concentration of aspergillus niger described above, genus bacillus and saccharomycetic dry powder goods is no less than 200 hundred million/grams.
Aspergillus niger described above, genus bacillus and saccharomycetic viable bacteria concentration are no less than the dry powder goods of 200 every gram hundred million, this is not difficult to obtain by understanding the growth properties of bacterial classification in the prior art, also existing method can be adopted to prepare simultaneously, the substratum of existing method choice optimization can be utilized to be cultivated by liquid culture fermentation equipment, then with high speed centrifugation, the thalline in fermented liquid is separated, be placed in dry indoor cold air drying or vacuum-drying mode bacterium mud is dry and obtain, the viable bacteria concentration of the thalline dry powder obtained is no less than the thalline dry powder of 200 every gram hundred million.
But, according to the bacterium mud that above-mentioned cultural method can obtain by the present invention equally, after testing, reach every gram containing viable bacteria concentration and be no less than 200 every gram hundred million, then can obstructed super-dry program and directly applying.
In above-mentioned one to three kind of scheme, described stirring, the technical qualification of aerated culture utilize electric mixing device, and arranging stirring velocity is 35-45r/min, and air flow is 1:0.6v/v/min.
In above-mentioned one to three kind of scheme, described centrifugation condition is centrifugal rotational speed is 6000-8000r/min, and centrifugation time is 15-20min.
Wort described above generally can be ordered to manufacturing enterprise's contract.But in most instances, prior art can be utilized to prepare voluntarily, its method preparing wort is: get 1 kilogram, Fructus Hordei Germinatus and add water 4.5 kilograms, 55 DEG C of insulations 1 hour, again temperature is risen to 62 DEG C and keep 5-6 hour, heat and boil rear iodine liquid inspection Glycation extend, reach more than 12 ° of Be ' pols, more than pH5.1, then can filter that to collect mashing water for subsequent use.The malt juice quality that this method is produced is stablized, and now in production application can make existing use, can save the trucking costs bought from professional production enterprise, reduce production cost.
The first step heated and boiled 2 hours sterilization process in above-mentioned first, second and third kind of scheme, effectively can increase the free of soluble substance and stripping, reheat and boil sterilizing in 1 hour, can ensure that nutrient solution sterilizing is complete further, be beneficial to the fermentation culture after access composite microbial bacteria, reduce the pollution of miscellaneous bacteria, improve the quality of goods.
" % " described in the present invention, than except another explaining, is all weight percentage.
Beneficial effect of the present invention:
1. fermentation of the present invention for the method for cell protein be directly utilize Ginkgo Leaf to decoct to extract after waste liquid after absorption with macroporous adsorbent resin, preparation method is easy, adapt to industrialization concentrated processing, the direct utilization ratio of its waste liquid is high, its process does not need operations such as concentrating, and power consumption is few, makes product cost low, there is actual production and using value, remarkable in economical benefits.
2. the present invention prepares the basic medium matter of cell protein is utilize the waste liquid in Ginkgo Leaf extraction flavones process, if the waste liquid of the processing enterprise of Folium Ginkgo extract is all obtained useful microbe conversion, there is provided considerable cell protein feed resource not only can to feed producer, the zero release of liquid waste disposal can be reached simultaneously, there is good social benefit and environmental benefit.
3. according to the first scheme implementation of the present invention, its waste liquid is after biological fermentation conversion, the output that can obtain cell protein is 10-11%, Folium Ginkgo extract manufacturing enterprise is closed on regard to locality, if be 10.5% calculating by mean yield, the productivity waste liquid that year produces is 31-62 ten thousand tons calculating, and can produce cell protein is 3.25-6.51 ten thousand tons; Implement according to first scheme, its waste liquid is after biological fermentation transforms, cell protein 11-12% can be obtained, if be 11.5% calculating by mean yield, just the local productivity waste liquid closing on the generation of Folium Ginkgo extract manufacturing enterprise year is 31-62 ten thousand tons calculating, and can produce cell protein is 3.56-7.13 ten thousand tons; According to the third scheme implementation, its waste liquid is after biological fermentation transforms, cell protein 18-20% can be obtained, if calculate by mean yield 19%, just the local productivity waste liquid closing on the generation of Folium Ginkgo extract manufacturing enterprise year is 31-62 ten thousand tons calculating, can produce cell protein is 5.89-11.78 ten thousand tons, and it is all very considerable that its economic worth and wasted resources are worth.
4. the waste liquid aboundresources during the present invention utilizes Folium Ginkgo extract to produce can obtain, its preparation technology is simple, it is low to consume energy, cost is cheap, non-pollution discharge, there is good implementation prospect, its product is also protein raw material in short supply at present, as the protein source of animal and fowl fodder, there are good market outlook.
Embodiment
Embodiment 1
The enforcement of the first scheme
Collect the waste liquid 85 kilograms after absorption with macroporous adsorbent resin ginkgolic flavone glycoside in Folium Ginkgo extract production, insert in fermentor tank, heated and boiled sterilizing in 2 hours, in time being cooled to 25-30 DEG C, pH to 4.8 is regulated with dilute phosphoric acid (98% strong phosphoric acid 1.5L is diluted with water to 40L), reheat and boil sterilizing in 1 hour, in time being cooled to 36-38 DEG C, access is by aspergillus niger, subtilis and cereuisiae fermentum are 1:1:3(and aspergillus niger 3 kilograms by weight, subtilis 3 kilograms, cereuisiae fermentum 9 kilograms) ratio mixes the complex microorganism bacterium leavening agent 15 kilograms be made into, it is 36 DEG C in temperature, ventilation is 1:0.6v/v/min, stirring velocity is under the condition of 35r/min, cultivation and fermentation is after 22 hours, disk centrifugal separator setting speed is adopted to be that 6000r/min carries out centrifuge dehydration 20min cultivation and fermentation liquid, collect solid and be placed in drip pan, 7% is less than to water content in 55 DEG C of vacuum-dryings, namely cell protein is obtained, the output of cell protein is 10.23 kilograms.
Embodiment 2
The enforcement of first scheme
Collect the waste liquid 78.745 kilograms after absorption with macroporous adsorbent resin ginkgolic flavone glycoside in Folium Ginkgo extract production, insert in fermentor tank, heated and boiled sterilizing in 2 hours, in time being cooled to 25-30 DEG C, add wort 5 kilograms, 1 kilogram, ammonium sulfate, four aqueous ferrous sulfates 0.015 kilogram, magnesium sulfate heptahydrate 0.04 kilogram, Calcium dichloride dihydrate 0.2 kilogram, stir, pH is regulated to be 5.0 with dilute phosphoric acid (98% strong phosphoric acid 1.5L is diluted with water to 40L), reheat and boil sterilizing in 1 hour, in time being cooled to 36-38 DEG C, access is by aspergillus niger, bacillus cereus and candida tropicalis mix by weight for 1:1:3 ratio the complex microorganism bacterium leavening agent 15 kilograms be made into, it is 38 DEG C in temperature, ventilation is 1:0.6v/v/min, stirring velocity is under the condition of 40r/min, cultivation and fermentation is after 20 hours, disk centrifugal separator setting speed is adopted to be that 7000r/min carries out centrifuge dehydration 18min cultivation and fermentation liquid, collect solid and be placed in drip pan, 7% is less than to water content in 55 DEG C of vacuum-dryings, namely cell protein is obtained, the output of cell protein is 11.62 kilograms.
Embodiment 3
The enforcement of the third scheme
Collect the waste liquid 73 kilograms after absorption with macroporous adsorbent resin ginkgolic flavone glycoside in Folium Ginkgo extract production, insert in fermentor tank, heated and boiled sterilizing in 2 hours, in time being cooled to 25-30 DEG C, add bran powder 12 kilograms, stir, pH is regulated to be 4.8 with dilute phosphoric acid (98% strong phosphoric acid 1.5L is diluted with water to 40L), reheat and boil sterilizing in 1 hour, in time being cooled to 36-38 DEG C, access is by aspergillus niger, Bacillus licheniformis and Candida utilis mix by weight for 1:1:3 ratio the complex microorganism bacterium leavening agent 15 kilograms be made into, it is 37 DEG C in temperature, ventilation is 1:0.6v/v/min, stirring velocity is under the condition of 45r/min, cultivation and fermentation is after 22 hours, disk centrifugal separator setting speed is adopted to be that 8000r/min carries out centrifuge dehydration 18min cultivation and fermentation liquid, collect solid and be placed in drip pan, 7% is less than to water content in 55 DEG C of vacuum-dryings, namely cell protein is obtained, the output of cell protein is 18.86 kilograms.
Embodiment 4
Use the substratum of corresponding optimization, by liquid fermentation and culture black-koji mould, then with supercentrifuge, the thalline in fermented liquid is separated, by vacuum lyophilization mode by dry for centrifugal bacterium mud out, obtain the aspergillus niger dry powder that viable bacteria content is 200 hundred million/grams.
Embodiment 5
Use the substratum of corresponding optimization, Bacillus licheniformis, bacillus cereus and subtilis is cultivated respectively by liquid, then with supercentrifuge, the thalline in fermented liquid is separated, by vacuum lyophilization mode, centrifugal bacterium mud out is dry, obtain Bacillus licheniformis, bacillus cereus and subtilis dry powder that viable bacteria content is 200 hundred million/grams respectively.
Embodiment 6
Use the substratum of corresponding optimization, cereuisiae fermentum and fodder yeast is cultivated respectively by liquid, then with supercentrifuge, the thalline in fermented liquid is separated, by vacuum lyophilization mode by dry for centrifugal bacterium mud out, obtain cereuisiae fermentum and feed yeast powder that viable bacteria content is 200 hundred million/grams respectively.
Embodiment 7
According to the enforcement of the first scheme
Collect the waste liquid 85 kilograms after absorption with macroporous adsorbent resin in Folium Ginkgo extract production, insert in fermentor tank, heated and boiled sterilizing in 2 hours, in time being cooled to 25-30 DEG C, pH is regulated to be 5.0 with dilute phosphoric acid (98% strong phosphoric acid 1.5L is diluted with water to 40L), reheat and boil sterilizing in 1 hour, in time being cooled to 36-38 DEG C, access the aspergillus niger obtained by embodiment 4, the Bacillus licheniformis that embodiment 5 obtains and the cereuisiae fermentum that embodiment 6 obtains mix by weight for 1:1:3 ratio the complex microorganism bacterium leavening agent 15 kilograms be made into, it is 38 DEG C in temperature, ventilation is 1:0.6v/v/min, stirring velocity is under the condition of 40r/min, cultivation and fermentation is after 18 hours, disk centrifugal separator setting speed is adopted to be that 7000r/min carries out centrifuge dehydration 18min cultivation and fermentation liquid, collect solid and be placed in drip pan, 7% is less than to water content in 55 DEG C of vacuum-dryings, namely cell protein is obtained, the output of cell protein is 11.15 kilograms.
Embodiment 8
According to the enforcement of first scheme
Collect the waste liquid 78.13 kilograms after absorption with macroporous adsorbent resin ginkgolic flavone glycoside in Folium Ginkgo extract production, insert in fermentor tank, heated and boiled sterilizing in 2 hours, in time being cooled to 25-30 DEG C, add wort 5 kilograms, 1.5 kilograms, ammonium sulfate, four aqueous ferrous sulfates 0.02 kilogram, magnesium sulfate heptahydrate 0.05 kilogram, Calcium dichloride dihydrate 0.3 kilogram, stir, pH is regulated to be 4.8 with dilute phosphoric acid (98% strong phosphoric acid 1.5L is diluted with water to 40L), reheat and boil sterilizing in 1 hour, in time being cooled to 36-38 DEG C, access the aspergillus niger obtained by embodiment 4, the bacillus cereus obtained by embodiment 5 and the fodder yeast obtained by embodiment 6 mix by weight for 1:1:3 ratio the complex microorganism bacterium leavening agent 15 kilograms be made into, it is 37 DEG C in temperature, ventilation is 1:0.6v/v/min, stirring velocity is under the condition of 40r/min, cultivation and fermentation is after 22 hours, disk centrifugal separator setting speed is adopted to be that 6000r/min carries out centrifuge dehydration 20min cultivation and fermentation liquid, collect solid and be placed in drip pan, 7% is less than to water content in 55 DEG C of vacuum-dryings, namely cell protein is obtained, the output of cell protein is 12.25 kilograms.
Embodiment 9
According to the enforcement of the third scheme
Collect the waste liquid 72 kilograms after absorption with macroporous adsorbent resin ginkgolic flavone glycoside in Folium Ginkgo extract production, insert in fermentor tank, heated and boiled sterilizing in 2 hours, in time being cooled to 25-30 DEG C, add Semen Maydis powder 13 kilograms, stir, pH is regulated to be 5.2 with dilute phosphoric acid (98% strong phosphoric acid 1.5L is diluted with water to 40L), reheat and boil sterilizing in 1 hour, in time being cooled to 36-38 DEG C, access the aspergillus niger obtained by embodiment 4, the subtilis obtained by embodiment 5 and the cereuisiae fermentum obtained by embodiment 6 mix by weight for 1:1:3 ratio the complex microorganism bacterium leavening agent 15 kilograms be made into, it is 38 DEG C in temperature, ventilation is 1:0.6v/v/min, stirring velocity is under the condition of 40r/min, cultivation and fermentation is after 20 hours, disk centrifugal separator setting speed is adopted to be that 7000r/min carries out centrifuge dehydration 20min cultivation and fermentation liquid, collect solid and be placed in drip pan, 7% is less than to water content in 55 DEG C of vacuum-dryings, namely cell protein is obtained, the output of cell protein is 20.16 kilograms.
Claims (10)
1. one kind utilize Folium Ginkgo extract to produce in waste liquid fermentation for the method for cell protein, it is characterized in that: the fresh waste liquid flowed out after absorption with macroporous adsorbent resin ginkgolic flavone glycoside during Folium Ginkgo extract is produced, insert in fermentor tank, heated and boiled sterilizing in 2 hours, in time being cooled to 25-30 DEG C, pH4.8-5.2 is regulated with dilute phosphoric acid, reheat and boil sterilizing in 1 hour, in time being cooled to 36-38 DEG C, obtain nutrient solution, complex microorganism bacterium leavening agent weight 15% is added by nutrient solution weight ratio, under 36-38 DEG C of condition, stir aerobic fermentation and cultivate 18-22 hour, after fermentation culture terminates, after fermentation liquor centrifuge dehydration, collect solid and be placed in drip pan, 7% is less than to water content in 55 DEG C of vacuum-dryings, obtain cell protein.
2. one kind utilize Folium Ginkgo extract to produce in waste liquid fermentation for the method for cell protein, it is characterized in that: the waste liquid flowed out after absorption with macroporous adsorbent resin ginkgolic flavone glycoside during Folium Ginkgo extract is produced, insert in fermentor tank, heated and boiled sterilizing in 2 hours, in time being cooled to 25-30 DEG C, add wort weight 5%, ammonium sulfate weight 0.5-1.5%, ferrous sulfate (FeSO by waste liquid weight ratio
4.4H
2o) weight 0.013-0.2%, magnesium sulfate (MgSO
4.7H
2o) weight 0.03-0.05%, calcium chloride (CaCl
2.2H
2o) weight 0.15-0.3%, stirs, and regulates pH4.8-5.2 with dilute phosphoric acid, reheat and boil sterilizing in 1 hour, in time being cooled to 36-38 DEG C, obtain nutrient solution, complex microorganism bacterium leavening agent weight 15% is added by nutrient solution weight ratio, under 36-38 DEG C of condition, stir aerated culture fermentation 18-22 hour, after cultivation and fermentation terminates, after fermentation liquor centrifuge dehydration, collect solid and be placed in drip pan, be less than 7% in 55 DEG C of vacuum-dryings to water content, obtain cell protein.
3. one kind utilize Folium Ginkgo extract to produce in waste liquid fermentation for the method for cell protein, it is characterized in that: the fresh waste liquid flowed out after absorption with macroporous adsorbent resin ginkgolic flavone glycoside during Folium Ginkgo extract is produced, insert in fermentor tank, heated and boiled sterilizing in 2 hours, in time being cooled to 25-30 DEG C, bran powder or the Semen Maydis powder of weight 12-15% is added by waste liquid weight ratio, stir, pH4.8-5.2 is regulated with dilute phosphoric acid, reheat and boil sterilizing in 1 hour, in time being cooled to 36-38 DEG C, obtain nutrient solution, complex microorganism bacterium leavening agent weight 15% is added by nutrient solution weight ratio, under 36-38 DEG C of condition, stir aerated culture fermentation 18-22 hour, after cultivation and fermentation terminates, after fermentation liquor centrifuge dehydration, collect solid and be placed in drip pan, 7% is less than to water content in 55 DEG C of vacuum-dryings, obtain cell protein.
4. as described in claim 1 or 2 or 3 utilize Folium Ginkgo extract to produce in waste liquid fermentation for the method for cell protein, it is characterized in that: described complex microorganism bacterium leavening agent is that aspergillus niger (Aspergillus niger), genus bacillus (Bacillus) and yeast (Yeast) are mixed in proportion composite forming, and its weight proportion is aspergillus niger: genus bacillus: yeast=1:1:3 ratio.
5. according to claim 4 utilize Folium Ginkgo extract to produce in waste liquid fermentation for the method for cell protein, it is characterized in that: described genus bacillus (Bacillus) is subtilis (Bacillus subtilis), Bacillus licheniformis (Bacillus licheniformis), Bacillus cereus (Bacillus cereus), Bacillus coagulans (Bacillus coagulans), any one in bacillus pumilus (Bacillus pumilus) and bacillus lentus (Slow bacillus).
6. according to claim 4 utilize Folium Ginkgo extract to produce in waste liquid fermentation for the method for cell protein, it is characterized in that: described yeast (Yeast) is candida tropicalis (Candida tropicalis), any one in Candida utilis (Candida utilis), cereuisiae fermentum (Beer yeast) and fodder yeast (Feeding yeast).
7. according to claim 4 or 5 or 6 utilize Folium Ginkgo extract to produce in waste liquid fermentation for the method for cell protein, it is characterized in that: described aspergillus niger, genus bacillus and yeast be commercially available edible or feeding containing viable bacteria concentration be 20,000,000,000/gram dry powder goods.
8. in utilizing Folium Ginkgo extract to produce according to claim 1-3, waste liquid fermentation is for the method for cell protein, it is characterized in that: described stirring, the condition of aerated culture are stirring velocity is 35-45r/min, and air flow is 1:0.6v/v/min.
9. in utilizing Folium Ginkgo extract to produce according to claim 1-3, waste liquid fermentation is for the method for cell protein, it is characterized in that: the condition of described centrifugation is rotating speed 6000-8000r/min, time 15-20min.
10. the cell protein product that the method as described in claim 1-10 obtains.
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