CN104559297A - Extraction method of high-coloring-power plant green pigment - Google Patents
Extraction method of high-coloring-power plant green pigment Download PDFInfo
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- CN104559297A CN104559297A CN201310508414.0A CN201310508414A CN104559297A CN 104559297 A CN104559297 A CN 104559297A CN 201310508414 A CN201310508414 A CN 201310508414A CN 104559297 A CN104559297 A CN 104559297A
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- enteromorpha
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B61/00—Dyes of natural origin prepared from natural sources, e.g. vegetable sources
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses an extraction method of high-coloring-power plant green pigment, which is characterized by comprising the following steps: (1) Enteromorpha filtrate extraction; (2) moss filtrate extraction; (3) centrifugal extraction; (4) constant-temperature ultrasonic extraction; and (5) macroporous resin adsorption. The algae and moss are used as the raw materials to extract the plant green pigment; and an ultrafiltration-macroporous resin adsorption combined process is adopted to enhance the pigment purity; the whole process has the advantages of low energy consumption, abundant raw material sources and low cost; and the pigment product has the advantages of fewer impurities, bright color, stable properties and high color value. The extraction method is simple, has the advantages of high operability and high extraction efficiency, and is easy for amplification and suitable for mass industrial production.
Description
Technical field
The invention belongs to technical field of pigment extraction, be specifically related to a kind of extracting method of green plant element of strong coloring force.
Background technology
Bryophyte is a kind of small-sized green plants, and structure is simple, only comprises stem and leaf two portions, sometimes only has flat thallus, do not have real root and vascular bundle.Bryophyte likes dark moist environment, generally grows on exposed cliff, or the forest of humidity and swampland.The internal structure of plant materials is simple, and rhizoid, by unicellular or be made up of 1 row cell, without center pillar, in higher kind, has the cell mass of similar transfusion tissue.Form, the structure of bryophyte body are simple even so, but break up like stem, leaf because bryophyte has, and spore distributes aloft, still has important biological significance to terrestrial organism.
The frond grass green of Enteromorpha, tubulose film quality, grows thickly, and major branch is obvious, and branch is elongated, Gao Keda 1 meter.Base portion is attached on rock with holdfast, grows at middle tide band beach, on chad.Due to the reason such as Global climate change, body eutrophication, the ocean green tide of kelp Enteromorpha is caused to break out.A large amount of Enteromorpha is floating gathers bank, blocks the fairway, destroys Marine ecosystems simultaneously, serious threat inshore fishery, tourism development.
In recent years, China is ripe gradually for the research and development of natural pigment, but still there is certain difficult point in pigment extraction.Adopt traditional leach extraction method, exist dissolvent residual, purity low, have peculiar smell, the drawback such as of poor quality, for leaching process brings very large puzzlement.Pigment pufification is inadequate, and tinting strength is not strong, poor stability.How the plant such as liver moss, Enteromorpha is used, extracts a kind of strong coloring force and the high pigment of stability is significant.
Summary of the invention
The object of this invention is to provide a kind of extracting method of green plant element of strong coloring force, improve pigment extraction yield, ensure the quality of pigment.
The invention provides a kind of extracting method of green plant element of strong coloring force, it is characterized in that, comprise the steps:
(1) Enteromorpha filtrate is extracted: get Enteromorpha drying and crushing to 100-120 order, add 0.3-0.5mol/L salt acid soak 50-60 min with solid-to-liquid ratio 1: 12-15, control microwave power at 700-800W, heating 120-130s, cold filtration after taking out, repetitive operation twice;
(2) liver moss filtrate is extracted: be the deionized water extraction 35-40min of 1: 10 by weight ratio at the temperature of 75-100 DEG C by liver moss, mixture to the PH value of above-mentioned thick slag and water is adjusted to be 4-5 with the hydrochloric acid that massfraction is 2%, at the temperature of 75-100 DEG C, insulation boiling said mixture 45-55min, filters and obtains extracting solution;
(3) Centrifugical extraction: Enteromorpha filtrate, liver moss filtrate are mixed according to quality 1:1: 5 with 80% ethanol, pH of mixed=3-4 is regulated with citric acid, mixed solution, at the centrifugal 25-30min of 4000r/min, gets the acid ethanol solution extraction of supernatant liquor pH=2-3;
(4) thermostatic ultrasonic extracts: extraction liquid adopts the ultra-filtration membrane of 0.1-0.2 μm, feeding temperature be 40-45 DEG C, under feed pressure is the condition of 0.2-0.3MPa, control ultrasonic power is 900-1000w, ultrasonic 20-25min;
(5) macroporous resin adsorption: adopt macroporous resin coutroi velocity to be that 3-4mL/min adsorbs above-mentioned ultrafiltrated, with the ethanol elution of 70%, concentrated, vacuum lyophilization 4-5h.
The 35-40% of liquid is concentrated in above-mentioned step (5).
The beneficial effect of the invention:
The present invention is with algae and liver moss for raw material extracts green plant element, and the method adopting ultrafiltration to combine with macroporous resin adsorption can improve pigment purity, whole process less energy consumption, abundant raw material source, cost is low, and pigment product impurity is few, lovely luster, stable in properties, look valency is high.Extracting method is simple, and workable, extraction efficiency is high, and technology is easy to amplify, and is suitable for large industrial production.
Embodiment
Embodiment 1
An extracting method for the green plant element of strong coloring force, is characterized in that, comprise the steps:
(1) extract Enteromorpha filtrate: get Enteromorpha drying and crushing to 120 order, add 0.5mol/L salt acid soak 50 min with solid-to-liquid ratio 1: 12, control microwave power at 700W, heating 130s, cold filtration after taking out, repetitive operation twice;
(2) liver moss filtrate is extracted: be the deionized water extraction 35min of 1: 10 by weight ratio at the temperature of 80 DEG C by liver moss, mixture to the PH value of above-mentioned thick slag and water is adjusted to be 4-5 with the hydrochloric acid that massfraction is 2%, at the temperature of 80 DEG C, insulation boiling said mixture 45min, filters and obtains extracting solution;
(3) Centrifugical extraction: Enteromorpha filtrate, liver moss filtrate are mixed according to quality 1:1: 5 with 80% ethanol, regulates pH of mixed=3-4 with citric acid, mixed solution, at the centrifugal 25min of 4000r/min, gets the acid ethanol solution extraction of supernatant liquor pH=2-3;
(4) thermostatic ultrasonic extracts: extraction liquid adopts the ultra-filtration membrane of 0.1 μm, feeding temperature be 40 DEG C, under feed pressure is the condition of 0.2MPa, control ultrasonic power is 1000w, ultrasonic 20min;
(5) macroporous resin adsorption: adopt macroporous resin coutroi velocity to be that 3mL/min adsorbs above-mentioned ultrafiltrated, with the ethanol elution of 70%, be concentrated into 35% of liquid, vacuum lyophilization 4h.
Embodiment 2
An extracting method for the green plant element of strong coloring force, is characterized in that, comprise the steps:
(1) extract Enteromorpha filtrate: get Enteromorpha drying and crushing to 120 order, add 0.3mol/L salt acid soak 60 min with solid-to-liquid ratio 1: 15, control microwave power at 800W, heating 120s, cold filtration after taking out, repetitive operation twice;
(2) liver moss filtrate is extracted: be the deionized water extraction 40min of 1: 10 by weight ratio at the temperature of 90 DEG C by liver moss, mixture to the PH value of above-mentioned thick slag and water is adjusted to be 4-5 with the hydrochloric acid that massfraction is 2%, at the temperature of 90 DEG C, insulation boiling said mixture 55min, filters and obtains extracting solution;
(3) Centrifugical extraction: Enteromorpha filtrate, liver moss filtrate are mixed according to quality 1:1: 5 with 80% ethanol, regulates pH of mixed=3-4 with citric acid, mixed solution, at the centrifugal 30min of 4000r/min, gets the acid ethanol solution extraction of supernatant liquor pH=2-3;
(4) thermostatic ultrasonic extracts: extraction liquid adopts the ultra-filtration membrane of 0.2 μm, feeding temperature be 45 DEG C, under feed pressure is the condition of 0.3MPa, control ultrasonic power is 900w, ultrasonic 25min;
(5) macroporous resin adsorption: adopt macroporous resin coutroi velocity to be that 4mL/min adsorbs above-mentioned ultrafiltrated, with the ethanol elution of 70%, be concentrated into 40% of liquid, vacuum lyophilization 5h.
Claims (2)
1. an extracting method for the green plant element of strong coloring force, is characterized in that, comprise the steps:
(1) Enteromorpha filtrate is extracted: get Enteromorpha drying and crushing to 100-120 order, add 0.3-0.5mol/L salt acid soak 50-60 min with solid-to-liquid ratio 1: 12-15, control microwave power at 700-800W, heating 120-130s, cold filtration after taking out, repetitive operation twice;
(2) liver moss filtrate is extracted: be the deionized water extraction 35-40min of 1: 10 by weight ratio at the temperature of 75-100 DEG C by liver moss, mixture to the PH value of above-mentioned thick slag and water is adjusted to be 4-5 with the hydrochloric acid that massfraction is 2%, at the temperature of 75-100 DEG C, insulation boiling said mixture 45-55min, filters and obtains extracting solution;
(3) Centrifugical extraction: Enteromorpha filtrate, liver moss filtrate are mixed according to quality 1:1: 5 with 80% ethanol, pH of mixed=3-4 is regulated with citric acid, mixed solution, at the centrifugal 25-30min of 4000r/min, gets the acid ethanol solution extraction of supernatant liquor pH=2-3;
(4) thermostatic ultrasonic extracts: extraction liquid adopts the ultra-filtration membrane of 0.1-0.2 μm, feeding temperature be 40-45 DEG C, under feed pressure is the condition of 0.2-0.3MPa, control ultrasonic power is 900-1000w, ultrasonic 20-25min;
(5) macroporous resin adsorption: adopt macroporous resin coutroi velocity to be that 3-4mL/min adsorbs above-mentioned ultrafiltrated, with the ethanol elution of 70%, concentrated, vacuum lyophilization 4-5h.
2. the extracting method of the green plant element of a kind of strong coloring force according to claim 1, is characterized in that, is concentrated into the 35-40% of liquid in described step (5).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310508414.0A CN104559297A (en) | 2013-10-25 | 2013-10-25 | Extraction method of high-coloring-power plant green pigment |
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| CN201310508414.0A CN104559297A (en) | 2013-10-25 | 2013-10-25 | Extraction method of high-coloring-power plant green pigment |
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| CN104559297A true CN104559297A (en) | 2015-04-29 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106397814A (en) * | 2016-09-21 | 2017-02-15 | 东莞市联洲知识产权运营管理有限公司 | Preparation method for nano heat insulation colored thin film based on plant pigment dyeing |
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2013
- 2013-10-25 CN CN201310508414.0A patent/CN104559297A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106397814A (en) * | 2016-09-21 | 2017-02-15 | 东莞市联洲知识产权运营管理有限公司 | Preparation method for nano heat insulation colored thin film based on plant pigment dyeing |
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Application publication date: 20150429 |