CN104556407A - Method for treating wastewater by immobilizing microorganisms to sisal hemp silk - Google Patents
Method for treating wastewater by immobilizing microorganisms to sisal hemp silk Download PDFInfo
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- CN104556407A CN104556407A CN201410846822.1A CN201410846822A CN104556407A CN 104556407 A CN104556407 A CN 104556407A CN 201410846822 A CN201410846822 A CN 201410846822A CN 104556407 A CN104556407 A CN 104556407A
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- sisal hemp
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Abstract
The invention relates to a method for treating wastewater by immobilizing microorganisms to sisal hemp silk. The method comprises the following steps: (1) performing high-temperature sterilization on the sisal hemp silk, namely washing the sisal hemp silk, and sterilizing to kill mixed bacteria on the sisal hemp silk; (2) treating the sisal hemp silk, namely adding water into flour to prepare paste, soaking the sterilized sisal hemp silk in the paste, taking out, drying, and adhering the flour to the sisal hemp silk; (3) soaking the treated sisal hemp silk in a microbial strain solution for treating the wastewater, and attaching the microorganisms to the sisal hemp silk; (4) putting the prepared sisal hemp silk attached with the microorganisms into the wastewater for treating the wastewater. The method for treating the wastewater by immobilizing the microorganisms to the sisal hemp silk as a carrier has the characteristics of low cost, simplicity and convenience for operation and high efficiency.
Description
Technical field
The invention belongs to environmental technology field, be specifically related to beneficial microorganism is fixed on the method for sisal hemp silk being administered sewage.
Background technology
China is populous, agriculture, industrial of a great variety, due to a large amount of discharges of sanitary sewage, rural sewage and industrial sewage etc., causes serious environmental pollution.Sewage disposal is a complicated engineering, relates to the integrated application of physics, chemistry and biotechnology.Although China establishes a lot of sewage work, but because sewage source is complicated, blowdown flow rate is large, the reasons such as sewage work's actual treatment sewage time is short, cost of sewage disposal is low, current wastewater treatment in China effect is also not obvious, cause the severe contamination of China's rivers and lakes, waste water control situation is very severe.In biological sewage treatment technology, multiple complex microorganism decomposing harmful substances is utilized to be main technology.In addition, in sewage treatment process, the selection of adherance is also very important.Adherance is the important support of the efficient Growth and Reproduction of microorganism.A kind of desirable adherance, need to possess a lot of condition, such as source is many and stable, reasonable price, long period of soaking, Resistance to microbes can be stood in water body, environment and microorganism be there is no to toxicity, a large amount of microorganisms can be adhered to thus form flora etc.Existing patent " the immobilized photosynthetic bacterium of a kind of Vegetable Sponge of Luffa or complex microorganism administer the method for lake blue algae " application number: CN201210579067; Open (bulletin) number: CN103058388A.It adopts Vegetable Sponge of Luffa as microbe carrier, but Vegetable Sponge of Luffa to there is source unstable, quantity is few, and the shortcoming of resistance to immersion difference in water.
Sisal hemp belongs to Agavaceae Agave, is a kind of perennial tropical hard leaf fibres crop, its original Mexico, existing main in Africa, Latin America, Asia etc. ground plant, be that world today's consumption is maximum, a kind of hard fiber widest in area.The country of current whole world plantation sisal hemp about has 20, and within 2006, world's sisal hemp output is 42.8 ten thousand tons.China is one of sisal hemp major country of production, is mainly distributed in the subtropical and tropical zones such as Guangdong, Guangxi, Hainan.The sisal hemp of China produces can trace back to for 20 beginnings of the century, but in the 1950's.By 2006, Chinese sisal hemp harvest area oneself reach 1.6 ten thousand hectares, ultimate production reaches 60,000 tons, accounts for 14% of the world, occupies second place of the world.Sisal hemp silky fibre is long, color and luster is pure white, strong but pliable in texture, high resilience, pulling force are strong, abrasion-resistant, acid and alkali-resistance, corrosion-resistant, the not easily feature such as skidding, be widely used in the industries such as fishery, navigation, industrial and mineral, transport, oil field, and for weaving sisal hemp carpet, one of sisal cloth, strainer, artwork etc.The Cable rope for ship pulling force wherein made with sisal hemp is strong, non-slip, sea water resistance soaks.Prior art does not have sisal hemp silk to be applied to the relevant report of sewage disposal as carrier at present.
Summary of the invention
Technical problem to be solved by this invention is to provide the method for the immobilized microbial treatment sewage of a kind of sisal hemp silk.
For solving the problems of the technologies described above, the technical solution used in the present invention is:
The method of the immobilized microbial treatment sewage of sisal hemp silk, comprises the following steps:
1) sisal hemp silk high-temperature sterilization process: cleaned by sisal hemp silk, through sterilising treatment, kills the miscellaneous bacteria on sisal hemp silk;
2) process of sisal hemp silk: flour is added water and is prepared into pasty state, put into by the sisal hemp silk after sterilizing and wherein soak, taking-up is dried, and makes flour stick on sisal hemp silk;
3) the sisal hemp silk after above-mentioned process is immersed in all liquid of improvement sewage microbial bacteria, by microorganism appendix on sisal hemp silk;
4) the sisal hemp silk of microorganism is had by the appendix prepared to be placed in sewage, for administering sewage.
By such scheme, described sterilising treatment is that clean sisal hemp silk is placed in high-temperature sterilization tank closed high temperature sterilising treatment, and the treatment time is 10-20 minute.
By such scheme, the soak time in described step (2) is 10-30 minute.
By such scheme, the soak time in described step (3) is 4-8h.
By such scheme, described microbial strains is based on the complex microorganism of the bacterial classification of bacillus and enterococcus spp.
By such scheme, all liquid of described microbial bacteria is cultivate in bacterium LB liquid nutrient medium microorganism strains being inoculated in standard to obtain.
By such scheme, the all liquid of described microbial bacteria is be inoculated in by microbial strains in the bacterium LB liquid nutrient medium of standard respectively, at 35-37 degree Celsius, 12-16h cultivated by shaking table that per minute 180-200 turns, to what be mixed to get after bacterium is in logarithmic phase (OD value 0.4-0.6).
By such scheme, there is by the appendix prepared the sisal hemp silk of microorganism to be fixed on floating log in described step (4), being then suspended in sewage, for administering sewage.
By such scheme, the sisal hemp silk after step 4) being used turns back in step 1) and regenerates.
The present invention is using sisal hemp silk as the immobilized microbial treatment sewage of carrier, due to sisal hemp silk steady sources, cheap, can obtain in enormous quantities, thus for the large-scale promotion of this sewage method provides basis; Sisal hemp silk is also highly stable in acid and alkali-resistance and other severe water surrounding sewage, can life-time service, is obviously superior to the adherances such as Vegetable Sponge of Luffa; Adopt flour to be attached on sisal hemp silk, the starch adhered to thus as the substratum of bacterium, can promote formation and the growth of bacteria flora, greatly improves the processing power to sewage.
Embodiment
Below in conjunction with example, the invention will be further described:
Example: the purification of water quality of eutrophication freshwater lake
Certain 1000 mu of lake, discharge for a long time due to neighbouring pig farm and do not have the sewage processed to lake, cause the main water quality deterioration in 60 meters of regions near the sewage draining exit of pig farm, index is: ammonia nitrogen 26.6mg/L; Dissolved oxygen 0.83mg/L; Biochemical oxygen demand (BOD) 54.1mg/L; Suspended substance 66mg/L.For polluting comparatively serious water quality.
Treatment step:
1) sisal hemp silk high-temperature disinfection treatment: cleaned by sisal hemp silk, is placed in water and boils process 20 minutes, kill the main miscellaneous bacteria on sisal hemp silk.
2) surface treatment of sisal hemp silk: add water into flour, reconcile into scattered paste shape, soaks wherein half hour by the sisal hemp silk disinfected, and takes out, natural air drying half.Flour sticks on sisal hemp silk, can be used as adherance and the substratum of bacterium.
3) the sisal hemp silk after above-mentioned process is immersed in subtilis, bacillus amyloliquefaciens, 4-8 hour in all liquid of composite microbial bacteria of enterococcus faecalis combination, by microorganism appendix on sisal hemp silk, described subtilis, bacillus amyloliquefaciens, the all liquid of composite microbial bacteria of enterococcus faecalis combination is by subtilis, bacillus amyloliquefaciens, enterococcus faecalis is inoculated in the bacterium LB liquid nutrient medium of standard respectively, at 35-37 degree Celsius, 12-16h cultivated by the shaking table that per minute 180-200 turns, to what be mixed to get after bacterium is in logarithmic phase (OD value 0.4-0.6).
4) there is by the appendix prepared the sisal hemp silk of composite microbial bacteria to be fixed in floating bed, be suspended in sewage draining exit annex lake surface, utilize the bacterial degradation process pollutant in water that sisal hemp adheres to.
5) improvement through about half a year, water quality reaches obvious improvement, and after administering, water-quality guideline is: ammonia nitrogen 1.6mg/L; Dissolved oxygen 2.83mg/L; Biochemical oxygen demand (BOD) 4.1mg/L; Suspended substance 16mg/L.
6) sisal hemp silk is through the immersion that can tolerate more than 1 year, need not change, more stable than other adherance.
Claims (9)
1. the method for the immobilized microbial treatment sewage of sisal hemp silk, is characterized in that: comprise the following steps:
1) sisal hemp silk high-temperature sterilization process: cleaned by sisal hemp silk, through sterilising treatment, kills the miscellaneous bacteria on sisal hemp silk;
2) process of sisal hemp silk: flour is added water and is prepared into pasty state, put into by the sisal hemp silk after sterilizing and wherein soak, taking-up is dried, and makes flour stick on sisal hemp silk;
3) the sisal hemp silk after above-mentioned process is immersed in all liquid of improvement sewage microbial bacteria, by microorganism appendix on sisal hemp silk;
4) the sisal hemp silk of microorganism is had by the appendix prepared to be placed in sewage, for administering sewage.
2. the method for the immobilized microbial treatment sewage of sisal hemp silk according to claim 1, is characterized in that: described sterilising treatment is that clean sisal hemp silk is placed in high-temperature sterilization tank closed high temperature sterilising treatment, and the treatment time is 10-20 minute.
3. the method for the immobilized microbial treatment sewage of sisal hemp silk according to claim 1, is characterized in that: the soak time in described step (2) is 10-30 minute.
4. the method for the immobilized microbial treatment sewage of sisal hemp silk according to claim 1, is characterized in that: the soak time in described step (3) is 4-8h.
5. the method for the immobilized microbial treatment sewage of sisal hemp silk according to claim 1, is characterized in that: described microbial strains is based on the complex microorganism of the bacterial classification of bacillus and enterococcus spp.
6. the method for the immobilized microbial treatment sewage of sisal hemp silk according to claim 1, is characterized in that: all liquid of described microbial bacteria is cultivate in bacterium LB liquid nutrient medium microorganism strains being inoculated in standard to obtain.
7. the method for the immobilized microbial treatment sewage of sisal hemp silk according to claim 1, it is characterized in that: all liquid of described microbial bacteria is be inoculated in by microbial strains in the bacterium LB liquid nutrient medium of standard respectively, at 35-37 degree Celsius, 12-16h cultivated by shaking table that per minute 180-200 turns, is in that logarithmic growth after date is mixed to get to bacterium.
8. the method for the immobilized microbial treatment sewage of sisal hemp silk according to claim 1, is characterized in that: being had by the appendix prepared the sisal hemp silk of microorganism to be fixed on floating log in described step (4), being then suspended in sewage, for administering sewage.
9. the method for the immobilized microbial treatment sewage of sisal hemp silk according to claim 1, is characterized in that: the sisal hemp silk after step 4) being used turns back in step 1) and regenerates.
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| CN201410846822.1A CN104556407A (en) | 2014-12-31 | 2014-12-31 | Method for treating wastewater by immobilizing microorganisms to sisal hemp silk |
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| CN201410846822.1A CN104556407A (en) | 2014-12-31 | 2014-12-31 | Method for treating wastewater by immobilizing microorganisms to sisal hemp silk |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1066832A (en) * | 1991-05-22 | 1992-12-09 | 华中理工大学 | Method for treating waste water containing phenol by fixed large edible fungus hypha |
| US5595893A (en) * | 1992-06-19 | 1997-01-21 | Iowa State University Research Foundation, Inc. | Immobilization of microorganisms on a support made of synthetic polymer and plant material |
| US20040084376A1 (en) * | 2000-03-24 | 2004-05-06 | Masatoshi Matsumura | Method for direct clarification of groundwater polluted with nitrate |
| CN1746115A (en) * | 2004-09-11 | 2006-03-15 | 中国石油化工股份有限公司 | Microbial filler and production thereof |
| CN1978639A (en) * | 2005-12-02 | 2007-06-13 | 南京理工大学 | Method for preparing immobilized thallus and its use |
| CN101665783A (en) * | 2009-09-27 | 2010-03-10 | 福建师范大学 | Method for preparing degradant of Venetian acinetobacter tetradecane by semisolid fermentation method based on using bagasse as immobilized carrier |
-
2014
- 2014-12-31 CN CN201410846822.1A patent/CN104556407A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1066832A (en) * | 1991-05-22 | 1992-12-09 | 华中理工大学 | Method for treating waste water containing phenol by fixed large edible fungus hypha |
| US5595893A (en) * | 1992-06-19 | 1997-01-21 | Iowa State University Research Foundation, Inc. | Immobilization of microorganisms on a support made of synthetic polymer and plant material |
| US20040084376A1 (en) * | 2000-03-24 | 2004-05-06 | Masatoshi Matsumura | Method for direct clarification of groundwater polluted with nitrate |
| CN1746115A (en) * | 2004-09-11 | 2006-03-15 | 中国石油化工股份有限公司 | Microbial filler and production thereof |
| CN1978639A (en) * | 2005-12-02 | 2007-06-13 | 南京理工大学 | Method for preparing immobilized thallus and its use |
| CN101665783A (en) * | 2009-09-27 | 2010-03-10 | 福建师范大学 | Method for preparing degradant of Venetian acinetobacter tetradecane by semisolid fermentation method based on using bagasse as immobilized carrier |
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Application publication date: 20150429 |