CN104543046B - Giant salamander oil natural composite antioxidant - Google Patents
Giant salamander oil natural composite antioxidant Download PDFInfo
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- CN104543046B CN104543046B CN201510050144.2A CN201510050144A CN104543046B CN 104543046 B CN104543046 B CN 104543046B CN 201510050144 A CN201510050144 A CN 201510050144A CN 104543046 B CN104543046 B CN 104543046B
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Abstract
The invention provides a giant salamander oil natural composite antioxidant which is composed of rosmarinic acid, bamboo leaf antioxidative substance and phytic acid, wherein the rosmarinic acid accounts for 0.01-0.02 wt% of the giant salamander oil, the bamboo leaf antioxidative substance accounts for 0.01-0.02% of the giant salamander oil, and the phytic acid accounts for 0.005-0.015% of the giant salamander oil. The application method comprises the following steps: proportionally weighing the rosmarinic acid, bamboo leaf antioxidative substance and phytic acid, dissolving by adding a small amount of anhydrous ethanol, adding giant salamander oil and evenly mixing. The natural composite antioxidant has high antioxidation effect for giant salamander oil, and has the advantages of no toxicity and high efficiency.
Description
Technical field
The present invention relates to a kind of antioxidant of animal fat, particularly a kind of antioxidant of giant salamander grease.
Background technology
Chinese giant salamander, Andrias davidianus (Andrias davidianus), belong to Amphibia, Caudata, Cryptobranchidae.With giant salamander scale
Change the success of chartering cost technology, giant salamander industry is increasingly becoming giant salamander main producing region and transforms agricultural production, promotes increasing peasant income
Most active new industry.At present giant salamander product is mainly used on dining table directly being eaten as high-end dish, consumption figure
It is less, the industrial chain development of restriction giant salamander, therefore how to promote giant salamander industrial chain to develop into the problem urgently explored.Giant salamander afterbody
Fat can not directly be eaten due to greasy, be that raw material extracts salamander oil with it, can improve the comprehensive utilization value of giant salamander, be promoted big
The exploitation of salamander deep processed product.But crude salamander oil salamander oil is rich in eicosapentaenoic acid（EPA）, DHA（DHA）Deng
Unsaturated fat absolute acid stability is poor, is easily oxidized, and generates the harmful substances such as aldehydes ketones, not only loses nutritive value, and
And certain toxic action is produced to human body, therefore delay the oxidation of salamander oil of crucial importance.
At present, the oxidation by adding antioxidants retard grease is generally acknowledged method the most cost-effective.With two
Butylhydroxy alcohol（BHA）, dibutyl hydroxy toluene（BHT）, tert-butylhydroquinone（TBHQ）Etc. artificial synthetized oxidation preventive agent
Side effect gradually studied confirmation, many countries have carried out the states such as strict control, the U.S., European Union to its addition and have prohibitted the use of
Synthetized oxidation preventive agent, research emphasis are also gradually transferred on the natural of safety non-toxic.Therefore, suitable oil is sought
Fat natural is particularly important.
Existing grease natural is a lot, such as natural A V, red chilli extract, spice extract, sugar alcohol
Class antioxidant etc.." a kind of natural composition as fish oil antioxidant " disclosed in Chinese patent application（CN1287157）,
Main anti-oxidant, reducing agent, oxygen scavenger, emulsifying agent are included, main anti-oxidant contains Tea Polyphenols, accounts for 66%-82%, reducing agent
Containing garlic oil, 5%-10% is accounted for, oxygen scavenger ketone group containing 3- glucofuranose lactone and its derivative, account for 4%-8%, emulsifying agent
Containing soybean lecithin, account for 9%-16%.The general addition of composition is 0.005%-0.1%.It has nontoxic, effective advantage, and
Can be used for already oxidised fish oil, delay its oxidation or recover its freshness.AOB, phytic acid, Tea Polyphenols are natural anti-
Oxidant, but their exclusive uses, it is not highly desirable to the antioxidant effect of giant salamander oil, and large usage quantity.
The content of the invention
It is an object of the invention to provide a kind of natural antioxidant composite of giant salamander oil, it has stronger antioxygen to salamander oil
Change effect, and non-toxic efficient.
For achieving the above object, embodiment of the present invention is：A kind of giant salamander oil natural composite antioxidant, by rosemary
Acid, AOB and phytic acid composition, the addition of Rosmarinic acid, AOB and phytic acid in giant salamander oil is respectively
0.01-0.02%, 0.01-0.02% and 0.005-0.015% of giant salamander oil quality.
As a kind of embodiment of optimization, the addition of Rosmarinic acid, AOB and phytic acid in giant salamander oil
Respectively 0.015%, 0.015% and the 0.007% of giant salamander oil quality.
The embodiment for alternatively optimizing, Rosmarinic acid, AOB and phytic acid adding in giant salamander oil
Dosage is respectively 0.015%, 0.01% and the 0.013% of giant salamander oil quality.
As the embodiment that the third optimizes, the addition of Rosmarinic acid, AOB and phytic acid in giant salamander oil
Amount is respectively 0.01,0.01% and the 0.01% of giant salamander oil quality.
As the embodiment of the 4th kind of optimization, the addition of Rosmarinic acid, AOB and phytic acid in giant salamander oil
Amount is respectively 0.02,0.01% and the 0.007% of giant salamander oil quality
The present invention provide giant salamander oil natural composite antioxidant using method be：Rosmarinic acid, bamboo are weighed in proportion
Leaf polyphenoils, phytic acid, add a small amount of anhydrous alcohol solution, add and are mixed in giant salamander oil.
The present invention antioxidation mechanism be：Rosmarinic acid is competitive with unrighted acid to be closed with lipid peroxy base junction, with
Terminate the chain reaction of lipid peroxidation, reduce lipid peroxidation, and Rosmarinic acid is oxidized to stable quinones substance.
Phytic acid can produce hydrogen and using phosphate group, chelated metal ions, destroy intermediate oxidation product, it is impossible to be allowed to form aldehyde, ketone
Product.AOB can remove free radical, the chain reaction of the fatty autoxidation of blocking.Although Rosmarinic acid, the leaf of bamboo resist
Oxide is differed with the mechanism of action of phytic acid three, but there is mutually coordinated, to be complementary to one another relation between them.Compounding is anti-
Raising antioxidant effect can be cooperateed between oxidant to there may be following reason：Can repair each other or regenerate, shape
Into redox cycle system, strengthen antioxidation activity；It is various during two or more antioxidant compounding use
Antioxidant is after anti-oxidant, it is also possible to so that the free radical for producing interacts generates new phenolic compound, continue to send out
Wave antioxidation so as to which antioxygenic property is enhanced.Coupled oxidation is acted on, and various antioxidants can be based on oxidation
The coupled oxidation of reduction potential difference, reduces the current potential drop between two kinds of agent of direct reaction, makes reaction be easy to carry out.Therefore, originally
The antioxidant effect of the anti-oxidant compositions that invention is provided is better than the effect of single antioxidant.
Description of the drawings
Fig. 1 is antioxidant effect of 0.02% antioxidant to salamander oil；
Fig. 2 is antioxidant effect of the Rosmarinic acid of Different adding amount to salamander oil；
Fig. 3 is antioxidant effect of the phytic acid of Different adding amount to salamander oil；
Fig. 4 is antioxidant effect of the AOB of Different adding amount to salamander oil；
Fig. 5 is that composite antioxidant and single antioxidant compare the antioxidant effect of salamander oil.
Specific embodiment
Embodiments of the invention are given below, the present invention is described in detail.
Embodiment 1：
A kind of giant salamander oil natural composite antioxidant, is made up of, rosemary Rosmarinic acid, AOB and phytic acid
The addition of acid, AOB and phytic acid in giant salamander oil is respectively the 0.015% of giant salamander oil quality, 0.015% and
0.007%。
The using method of above-mentioned giant salamander oil natural composite antioxidant is：It is anti-oxidant that Rosmarinic acid, the leaf of bamboo are weighed in proportion
Thing, phytic acid, add a small amount of anhydrous alcohol solution, are then added to be mixed in fish oil.
Embodiment 2：
A kind of giant salamander oil natural composite antioxidant, is made up of, rosemary Rosmarinic acid, AOB and phytic acid
The addition of acid, AOB and phytic acid in giant salamander oil is respectively the 0.015% of giant salamander oil quality, 0.01% and
0.013%.Using method is same as Example 1.
Embodiment 3：
A kind of giant salamander oil natural composite antioxidant, is made up of, rosemary Rosmarinic acid, AOB and phytic acid
The addition of acid, AOB and phytic acid in giant salamander oil is respectively 0.01%, 0.01% and the 0.01% of giant salamander oil quality.
Using method is same as Example 1.
Embodiment 4：
A kind of giant salamander oil natural composite antioxidant, is made up of, rosemary Rosmarinic acid, AOB and phytic acid
The addition of acid, AOB and phytic acid in giant salamander oil is respectively 0.02%, 0.01% and the 0.007% of giant salamander oil quality.
Using method is same as Example 1.
Embodiment 5：The screening of giant salamander oil natural composite antioxidant and Oxidation Resistance Test.
1 materials and methods
1.1 materials and reagent
Giant salamander tail fatty oil（Laboratory is made by oneself, and without antioxidant, initial p OV is 2.46 mmol/kg）.
Rosmarinic acid（Purity 10%）；AOB（Purity 98%）；Phytic acid（Purity 10%）Tea Polyphenols（Purity 85%）；
Tert-butylhydroquinone（TBHQ purity >=99%）.It is pure that other reagents are analysis.
1.2 instrument and equipment
Medical Equipment Plant of GZX-9146MBE digital display constant temperature blast drying ovens Shanghai Boxun Industrial Co., Ltd.；HH- is serial
Jin Cheng Guo Sheng laboratory apparatus factory of Jintan City of thermostat water bath Jiangsu Province；Section's instrument and meter is helped in JA2003N type analysis balances Shanghai
Co., Ltd；
1.3 method
1.3.1 the measure sodium thiosulfate titration (with reference to GB/T5538-2005) of fish oil peroxide value[10], each sample
Product survey three times it is parallel.
1.3.2 anti-oxidant research of the different antioxidants to fish oil
Weigh 20 g（It is accurate to 0.001g）Salamander oil, adds respectively 0.02% AOB, Tea Polyphenols, rosemary
Acid, 4 kinds of naturals of phytic acid, first use a small amount of anhydrous alcohol solution, add in fish oil and mix, while with blank sample（No
Addition antioxidant）Control, it is open to be placed in 60 ± 1 DEG C of thermostatic drying chamber using Schaal Oven Methods, every 24 h stirrings
Once, the peroxide value of oil sample and is determined respectively.
1.3.3 the determination of the most suitable addition of natural
20 g salamanders oil is weighed, is added respectively different amounts of（0.02%、0.04%、0.06%、0.08%）Antioxidant, adopts
Schaal Oven Methods, it is open to be placed in 60 ± 1 DEG C of thermostatic drying chamber, stir once every 12 h, and oil sample is determined respectively
Peroxide value.
1.3.4 the composition research of natural
Compounded from the preferable 3 kinds of naturals of effect, designed L9（34）Orthogonal arrage, using Schaal baking ovens
Method is tested, and with peroxide value during 80 h of fish oil as index, determines the use of single antioxidant in compound antioxidant
Amount.During compounding test, the usage amount of every kind of natural can only be the 1/3 of every kind of antioxidant optimum consumption.
1.3.5 the comparison to fish oil antioxidant effect with single antioxidant is compounded
The compound antioxidant of above-mentioned optimization is contrasted with the most suitable addition of single antioxidant, and is added respectively
Identical synergist, mixes, while the TBHQ for arranging 0.02% is control group experiment, it is open to be placed in 60 using Schaal Oven Methods
In ± 1 DEG C of thermostatic drying chamber, stir once every 24 h, and determine the peroxide value of oil sample respectively.
Data processing is analyzed process using SPSS20.0 softwares, and representation is " means standard deviation ".
2 results and analysis
Antioxidant effect of the 2.1 different antioxidants to giant salamander oil
As shown in Figure 1, above-mentioned various antioxidants can delay the oxidation of salamander oil, and in addition 0.02% situation is
Under, less, from the 5th day, each antioxidant was as follows to salamander oil antioxidant effect for the gap of first 4 days：Phytic acid>AOB
>Rosemary>Tea Polyphenols.In natural, preferably, Tea Polyphenols antioxidant effect is worst, may be with them for the effect of phytic acid
Purity it is relevant with structure-activity relationship.
The determination of the most suitable addition of 2.2 naturals
From Fig. 2 ~ Fig. 4, with the increase of Rosmarinic acid, AOB and phytic acid addition, they are to salamander oil
Antioxidant effect strengthen, when Rosmarinic acid and AOB addition reach more than 0.06% and phytic acid addition reach
During to more than 0.06%, the variation tendency of salamander oil peroxide value is less obvious, therefore, it is comprehensive in terms of antioxidant effect and cost
Consider, determine that the most suitable addition of Rosmarinic acid, AOB and phytic acid is respectively 0.06%, 0.06% and 0.04%.Respective
Under most suitable addition, antioxidant effect is as follows：Rosmarinic acid>Phytic acid>AOB.The most suitable addition of antioxidant and effect
The difference of fruit, the amount of this active ingredient contained with selected antioxidant itself has very big relation, also with effectively into
The free radical absorbability divided is closely related, while the impact of non-active ingredients can not be excluded.
Comparison of 2.3 compoundings with single antioxidant to fish oil antioxidant effect
As shown in Figure 1, the peroxide value of blank sample amplification in 3d is larger, therefore, the peroxide value with the 80th h is
Index, orthogonal experiment factor is shown in Table 1 with level.As shown in Table 2, Orthogonal experiment results are analyzed, affect fish oil anti-oxidant
The secondary factors of effect are C>A>B, i.e. phytic acid>Rosmarinic acid>AOB, draws the preferable combination of compound antioxidant
For A2B2C3、A2B3C1、A3B3C2With A1B3C3, POV is 5.88 ± 0.07,5.90 ± 0.12 and 6.00 ± 0.14, is orthogonal experiment
In relatively low-peroxide value, therefore select compound antioxidant better formula be the leaf of bamboo of 0.015% Rosmarinic acid+0.015% resist
The phytic acid of oxide+0.007%, the phytic acid of+0.01% AOB of 0.015% Rosmarinic acid+0.013%, 0.01% Rosmarinic acid+
The phytic acid of 0.01% AOB+0.01% and+0.01% AOB of 0.02% Rosmarinic acid 4 kinds of groups of+0.007% phytic acid
Compound antioxidant is produced respectively in conjunction.
The orthogonal experiment factor of table 1 and level
| Level | A Rosmarinic acids/% | B AOBs/% | C phytic acid/% |
| 1 | 0.02 | 0.02 | 0.013 |
| 2 | 0.015 | 0.015 | 0.01 |
| 3 | 0.01 | 0.01 | 0.007 |
The orthogonal experiments of table 2 and analysis
| Tested number | A | B | C | POV/(mmol/kg) |
| 1 | 1 | 1 | 1 | 7.66±0.44 |
| 2 | 1 | 2 | 2 | 6.89±0.16 |
| 3 | 1 | 3 | 3 | 6.00±0.14 |
| 4 | 2 | 1 | 2 | 6.46±0.33 |
| 5 | 2 | 2 | 3 | 5.88±0.07 |
| 6 | 2 | 3 | 1 | 5.90±0.12 |
| 7 | 3 | 1 | 3 | 6.28±0.01 |
| 8 | 3 | 2 | 1 | 6.86±0.07 |
| 9 | 3 | 3 | 2 | 5.90±0.12 |
| k1 | 6.85 | 6.68 | 6.84 | |
| k2 | 6.31 | 6.35 | 6.74 | |
| k3 | 6.35 | 6.48 | 5.93 | |
| R | 0.54 | 0.33 | 0.91 |
Fig. 5 is shown in complex natural antioxidant, single antioxidant and TBHQ contrast, it is seen then that compound antioxidant is to fish oil
Antioxidant effect be substantially better than single antioxidant, antioxidant effect（The leaf of bamboo of 0.02% Rosmarinic acid+0.01% is anti-oxidant
The phytic acid of thing+0.007%）＞（The phytic acid of+0.01% AOB of 0.015% Rosmarinic acid+0.013%）＞（0.01% Rosmarinic acid
The phytic acid of+0.01% AOB+0.01%）＞（The phytic acid of+0.01% AOB of 0.02% Rosmarinic acid+0.007%）,
Close synthetized oxidation preventive agent TBHQ, China《Food additives use sanitary standard》There is strict restriction to TBHQ additions, typically
No more than 0.02%, therefore, from from the aspect of safety and effect, compound antioxidant can suitably replace TBHQ.
Claims (6)
1. a kind of giant salamander oil natural composite antioxidant, it is characterised in that by Rosmarinic acid, AOB and phytic acid group
Into the addition of Rosmarinic acid, AOB and phytic acid in giant salamander oil is respectively the 0.01%- of giant salamander oil quality
0.02%th, 0.01%-0.02% and 0.005%-0.015%.
2. giant salamander according to claim 1 oil natural composite antioxidant, it is characterised in that Rosmarinic acid, leaf of bamboo antioxygen
The addition of compound and phytic acid in giant salamander oil is respectively 0.015%, 0.015% and the 0.007% of giant salamander oil quality.
3. giant salamander according to claim 1 oil natural composite antioxidant, it is characterised in that Rosmarinic acid, leaf of bamboo antioxygen
The addition of compound and phytic acid in giant salamander oil is respectively 0.015%, 0.01% and the 0.013% of giant salamander oil quality.
4. giant salamander according to claim 1 oil natural composite antioxidant, it is characterised in that Rosmarinic acid, leaf of bamboo antioxygen
The addition of compound and phytic acid in giant salamander oil is respectively 0.01%, 0.01% and the 0.01% of giant salamander oil quality.
5. giant salamander according to claim 1 oil natural composite antioxidant, it is characterised in that Rosmarinic acid, leaf of bamboo antioxygen
The addition of compound and phytic acid in giant salamander oil is respectively 0.02%, 0.01% and the 0.007% of giant salamander oil quality.
6. it is a kind of as described in claim 1-5 any one giant salamander oil natural composite antioxidant using method, its feature
It is to weigh Rosmarinic acid, AOB, phytic acid in proportion, adds a small amount of anhydrous alcohol solution, in adds giant salamander oil
Mix.
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| CN101422201A (en) * | 2008-11-18 | 2009-05-06 | 长春工业大学 | Natural compound antioxidant for fat-rich food |
| CN102041169A (en) * | 2010-12-23 | 2011-05-04 | 厦门金达威集团股份有限公司 | Antioxidant composition suitable for unsaturated fatty acid and ester thereof |
| CN102178281B (en) * | 2011-03-23 | 2012-12-12 | 荣成百合生物技术有限公司 | Antioxidative composition for fish oil |
| CN103609754B (en) * | 2013-12-04 | 2015-04-22 | 安徽农业大学 | Paeonia ostii seed oil with oxidative stability and preparation method thereof |
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