CN104531605A - Method for promoting methanogenic bacteria to grow by use of polymetal-layered double hydroxides (PM-LDH) prepared from heavy metal-containing wastewater - Google Patents

Method for promoting methanogenic bacteria to grow by use of polymetal-layered double hydroxides (PM-LDH) prepared from heavy metal-containing wastewater Download PDF

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Publication number
CN104531605A
CN104531605A CN201410823629.6A CN201410823629A CN104531605A CN 104531605 A CN104531605 A CN 104531605A CN 201410823629 A CN201410823629 A CN 201410823629A CN 104531605 A CN104531605 A CN 104531605A
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ldh
methanogen
heavy metal
add
methanogenic bacteria
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刘建勇
钱光人
孙浩源
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University of Shanghai for Science and Technology
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University of Shanghai for Science and Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound

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Abstract

The invention discloses a method for promoting methanogenic bacteria to grow by use of polymetal-layered double hydroxides (PM-LDH) prepared from heavy metal-containing wastewater, and belongs to the technical field of environmental protection. According to the method, the PM-LDH prepared from metal ions in the heavy metal-containing wastewater is fed into a culture solution containing the methanogenic bacteria, and the addition of the PM-LDH is found to have a certain promoting effect on the growth of the methanogenic bacteria, and the addition of the PM-LDH is capable of increasing the growth amount of the methanogenic bacteria by about 15%. The method for promoting the methanogenic bacteria to grow by use of the PM-LDH prepared from the heavy metal-containing wastewater is capable of well solving the problems of lack of nutrient microelements and nutritive proportion imbalance in the anaerobic biological treatment process of high concentrated organic wastewater, and improving the anaerobic biological treatment efficiency of the high concentrated organic wastewater.

Description

The method of the PM-LDH utilizing heavy metal-containing waste water to prepare in order to promote methanogen to grow
Technical field
The invention belongs to field of Environment Protection, relate to a kind of polynary layered metal hydroxides (PM-LDH) method in order to promote methanogen to grow utilizing heavy metal-containing waste water to prepare, the PM-LDH specifically utilizing heavy metal wastewater thereby to prepare promotes the growth of methanogen.
Background technology
High concentrated organic wastewater anaerobic biological treatment is the important channel of sewage recycling process, and methanogen is the major function microorganism that organism anaerobic methaneization produces methane.But methanogen growth fraction is slower, simultaneously harsher to the required environmental requirement of growth, need multiple a small amount of and micro-metals, as mainly contained Fe, Co, Ni, Zn, Cu, Mn, Mo, Se, W etc., these metallic elements serve activation to methanogen in high concentrated organic wastewater anaerobic treatment process.
But, lack these micronutrient elements and out of proportion be FAQs in many high concentrated organic wastewater anaerobic biological treatment processes.Research shows, most of high concentrated organic wastewater, comprise food industrial wastewater, beverage industrial waste water and wastewater from fermentation industry all can not reach and make the nutritive equilibrium of anaerobic treatment required by optimum condition, anaerobic treatment process must add the nutrition that metallic element comes needed for supplementary methanogen in right amount in waste water.But needed for methanogen there is different shape in metallic element in anaerobic system, and the bioavaliability of different shape metallic element is distinguishing, directly add in waste water ionic state metallic element may precipitated, be chelated, adsorbed thus cause its bioavaliability to reduce.Meanwhile, excessive interpolation metallic element also can produce restraining effect to methanogen activity.
LDH is a kind of anionic clay materials with unique laminate structure, and main body laminate is mainly divalence, trivalent metal cation.LDH can in water slow releasing metal ion, it can be continued in theory provide methanogen grow needed for metallic element.
Summary of the invention
The object of the invention is the problem that the metal nutritive element that will solve in high concentrated organic wastewater can not meet bio anaerobic process growth demand, provide and utilize heavy metal wastewater thereby to prepare PM-LDH, put into high concentrated organic wastewater anaerobic system, the Determination of multiple metal elements of PM-LDH slow releasing can promote the growth of methanogen.
The technical solution used in the present invention is as follows:
A kind of polynary layered metal hydroxides (PM-LDH) method in order to promote methanogen to grow utilizing heavy metal-containing waste water to prepare, it is characterized in that there is following process and step: the preparation of A. PM-LDH: a. get containing Co 60 ~ 400 mg/L, Ni 300 ~ 500 mg/L, Cu 50 ~ 200 the waste water of the metallic element such as mg/L be object, get above-mentioned waste water 50-100ml and add uniform stirring at 50ml distilled water 25 DEG C; And add 0.28M sodium hydroxide adjustment pH to 8.0 ~ 9.0 wherein; Meanwhile, CO is passed into wherein 2and abundant aeration 1 hour; B., after aeration terminates, suspension is vibrated 16 ~ 24 hours at 80 DEG C; After vibration terminates, this suspension suction filtration is rinsed repeatedly with deionized water, puts into 60 DEG C of oven drying 24h subsequently, thus obtained required particle diameter is the PM-LDH particle of 0.2mm ~ 0.3mm; B. the method for methanogen growth is promoted with PM-LDH: a. contains the nutrient solution 2mL of methanogen after getting activation, be inoculated in 150mL synthetic medium; Consisting of of substratum: glucose 10g/L, (NH 4) 2sO 40.5g/L, K 2hPO 43.5g/L, KH 2pO 41.5g/L; And amount of makeup water is to 1000mL; The packing of 250mL Erlenmeyer flask; Substratum uses high-pressure sterilizing pot at 121 DEG C of sterilizing about 20min in advance; Cool stand-by; B. in the above-mentioned substratum containing methanogen, adding particle diameter is 0.2mm ~ 0.3mm, and concentration is the PM-LDH of 1g/L; Add the speed of growth of the methanogen of PM-LDH particle, with do not add polynary stratiform metal hydroxide particle methanogen the speed of growth compared with, methanogen increment improve about 15%.
Embodiment
After the specific embodiment of the invention is now described in by embodiment 1.
Get containing Co(60 ~ 400 mg/L), Ni(300 ~ 500 mg/L), Cu(50 ~ 200 mg/L) etc. the waste water 50ml of metallic element add uniform stirring at 50ml distilled water 25 DEG C, and add 0.28M sodium hydroxide adjustment pH to 9.0 wherein, meanwhile, CO is passed into wherein 2and abundant aeration 1 hour.After aeration terminates, suspension is vibrated 24 hours at 80 DEG C.After vibration terminates, repeatedly rinsed by this suspension suction filtration, put into 60 DEG C of oven drying 24h subsequently with deionized water, after grinding, obtained required particle diameter is the PM-LDH particle of 0.2mm ~ 0.3mm.
By the nutrient solution 2mL containing methanogen after activation, be inoculated in 150mL synthetic medium; Consisting of of substratum: glucose 10g/L, (NH 4) 2sO 40.5g/L, K 2hPO 43.5g/L, KH 2pO 41.5g/L amount of makeup water is to 1000mL; The packing of 250mL Erlenmeyer flask; Substratum uses high-pressure sterilizing pot at 121 DEG C of sterilizing about 20min in advance; Cool stand-by; And at 35 DEG C, shaking culture 36 h under 150r/min condition; Then add to containing in the substratum of methanogen the PM-LDH particle that particle diameter is 0.2mm ~ 0.3mm, concentration is 1g/L, and contrast with the carrying out not adding PM-LDH particle.
By measuring the OD of two experimental group methanogens 660value, find 3 ~ 12h after adding PM-LDH, the speed of growth adding the methanogen of PM-LDH experimental group is compared with control group and is all shown obvious advantage, add PM-LDH experimental group methanogen increment and do not add PM-LDH control group methanogen increment raising about 15%, and reach maximum value and keep more stable state.

Claims (1)

1. utilize heavy metal-containing waste water to prepare polynary layered metal hydroxides (PM-LDH) method in order to promote methanogen to grow, it is characterized in that there is following process and step: the preparation of A. PM-LDH: a. get containing Co 60 ~ 400 mg/L, Ni 300 ~ 500 mg/L, Cu 50 ~ 200 the waste water 50-100ml of mg/L add uniform stirring at 50ml distilled water 25 DEG C; And add 0.28M sodium hydroxide adjustment pH to 8.0 ~ 9.0 wherein; Meanwhile, CO is passed into wherein 2and abundant aeration 1 hour; B. after aeration terminates, to be vibrated 16 ~ 24 hours by suspension at 80 DEG C, after vibration terminates, repeatedly rinsed by this suspension suction filtration, put into 60 DEG C of oven drying 24h subsequently with deionized water, obtained required particle diameter is the PM-LDH particle of 0.2mm ~ 0.3mm; B. promote the method for methanogen growth with PM-LDH, a. contains the nutrient solution 2mL of methanogen after getting activation, be inoculated in 150mL synthetic medium; Consisting of of substratum: glucose 10g/L, (NH 4) 2sO 40.5g/L, K 2hPO 43.5g/L, KH 2pO 41.5g/L; And amount of makeup water is to 1000mL; The packing of 250mL Erlenmeyer flask; Substratum uses high-pressure sterilizing pot at 121 DEG C of sterilizing about 20min in advance; Cool stand-by; B. in the above-mentioned substratum containing methanogen, adding particle diameter is 0.2mm ~ 0.3mm, and concentration is the PM-LDH of 1g/L; Add the speed of growth of the methanogen of PM-LDH particle, with do not add polynary stratiform metal hydroxide particle methanogen the speed of growth compared with, methanogen increment improve about 15%.
CN201410823629.6A 2014-12-26 2014-12-26 Method for promoting methanogenic bacteria to grow by use of polymetal-layered double hydroxides (PM-LDH) prepared from heavy metal-containing wastewater Pending CN104531605A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113092553A (en) * 2021-03-03 2021-07-09 哈尔滨工业大学（深圳） Novel method for detecting glucose by enzyme-free glucose sensor

Citations (3)

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Publication number Priority date Publication date Assignee Title
CN201339033Y (en) * 2009-01-04 2009-11-04 上海大学 Novel device for generating hydrogen and methane
CN101905869A (en) * 2009-06-05 2010-12-08 北京化工大学 Method for preparing laminated composite metal hydroxide
CN102140001A (en) * 2010-12-17 2011-08-03 合肥工业大学 Method for increasing methane production efficiency in organic waste anaerobic process

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN201339033Y (en) * 2009-01-04 2009-11-04 上海大学 Novel device for generating hydrogen and methane
CN101905869A (en) * 2009-06-05 2010-12-08 北京化工大学 Method for preparing laminated composite metal hydroxide
CN102140001A (en) * 2010-12-17 2011-08-03 合肥工业大学 Method for increasing methane production efficiency in organic waste anaerobic process

Non-Patent Citations (4)

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Title
FAZHI ZHANG ET AL: "Layered double hydroxides as catalytic materials: recent development", 《CATAL SURV ASIA》 *
JI ZHI ZHOU ET AL: "Formation of layered double hydroxides in self-purification of polynary metal electroplating wastewater for effective removal of anionic dye", 《TMS》 *
祖波 等: "产甲烷菌的生理生化特性", 《环境科学与技术》 *
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113092553A (en) * 2021-03-03 2021-07-09 哈尔滨工业大学（深圳） Novel method for detecting glucose by enzyme-free glucose sensor

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Application publication date: 20150422