CN104531334A - Clean production process for simultaneously extracting lipid and collagen from animal fat tissues - Google Patents
Clean production process for simultaneously extracting lipid and collagen from animal fat tissues Download PDFInfo
- Publication number
- CN104531334A CN104531334A CN201410834564.5A CN201410834564A CN104531334A CN 104531334 A CN104531334 A CN 104531334A CN 201410834564 A CN201410834564 A CN 201410834564A CN 104531334 A CN104531334 A CN 104531334A
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- China
- Prior art keywords
- adipose tissue
- animal
- collagen protein
- lipid
- animal adipose
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
Abstract
The invention belongs to the technical field of livestock and poultry processing, and in particular relates to a clean production process for simultaneously extracting lipid and collagen from animal fat tissues. The clean production process provided by the invention is simple, convenient and efficient, is low in cost and high in added value and is used for processing and utilizing the animal fat tissues, and by using the process, collagen can be industrially extracted from the animal fat tissues on a large scale, and animal fat can be obtained at the same time. Compared with the prior art, by using the process provided by the invention, the animal fat tissues are used as a raw material for extracting collagen, high-quality collagen and animal fat can be obtained on the premise that wastewater is not generated, green and clean utilization of the animal fat tissues are realized, a big pollutant source of the livestock and poultry processing industry is eliminated, and meanwhile, the added value of the animal fat tissues is also greatly improved, so that the clean production process has a wide application prospect in the field of livestock and poultry processing.
Description
Technical field
The invention belongs to the processing technique field of livestock and poultry, belong to the process for cleanly preparing simultaneously extracting lipid and collagen protein in a kind of animal adipose tissue specifically.
Background technology
Along with the continuous expansion of China's animal husbandry scale, animal adipose tissue has become a kind of resource of Gong processing and utilization of animal-origin.Due to animal tallow containing saturated fatty acid and cholesterol more, too much eat and easily cause hypertension, arteriosclerosis, coronary heart disease, hyperlipidaemia and cerebrovascular accident, unfavorable to human body, thus fat meat is not more and more by the favor of human consumer, sales situation is bad, and price is expected to fall, has had influence on the overall efficiency of butchering processing enterprise, be necessary that new processing and utilization thinking and complete processing are set up in exploratory development, improve the added value of animal adipose tissue.
The existing method extracting animal tallow in animal adipose tissue has pyretic exercise, low temperature wet method and high temperature wet, and all these complete processings are all be extracted as object only with animal tallow, are never conceived to the exploitation of reticular tissue in animal adipose tissue.
Summary of the invention
A kind of process for cleanly preparing simultaneously extracting lipid and collagen protein from animal adipose tissue of invention.Comparatively unique, it, by by reticular tissue (mainly collagen protein) hydrolysis in animal adipose tissue, makes animal grease discharge, achieves the object simultaneously obtaining animal grease and collagen protein from animal adipose tissue.Operate according to this patent, compared with traditional method before this, can under the prerequisite of the quality and productive rate that do not reduce animal grease, the acquisition collagen product of high-quality high yield.This improvement is vital for the comprehensive utilization of animal adipose tissue resource, and the effective rate of utilization of resource can be made to greatly increase, and greatly improves the producing level of animal adipose tissue resource, increases utility value.
One of most important object of the present invention, is to solve cleaning and environmental friendliness of animal tallow production, makes the production of animal tallow no longer produce waste water and waste residue.As one, along the result be with, another object of the present invention is the recycling problem of the reticular tissue part that will realize animal adipose tissue, to reduce production cost, improves the utility value of animal adipose tissue.
Specifically, first, we find that the reticular tissue in animal adipose tissue has good degradability, can be dissolved by media such as alkaline ethanol, enzyme liquid, basic solutions.Under the processing condition that we take, animal adipose tissue can all dissolve and be discharged by animal tallow, and can AUTOMATIC ZONING under suitable condition.Establish thus and in the mode that reticular tissue is dissolved, animal adipose tissue has been processed, extracted the basis of lipid and collagen protein simultaneously.
Secondly, we carry out cold soaking as solvent to animal adipose tissue by the alkaline low carbon alcohol solution of discovery suitable concentration, effectively can be dissolved in aqueous under the prerequisite keeping the basic unchangeability of collagen protein, separate with animal lipid, the indexs such as the color and luster of gained animal lipid, smell, function quality and security are significantly improved.
We find, animal lipid and collagen protein extract by the technique be made up of the following step and obtain:
1) animal adipose tissue mincer is rubbed;
2) reticular tissue in animal adipose tissue is hydrolyzed, the reticular tissue in animal adipose tissue is dissolved, form collagen aqueous solution, the fat in animal adipose tissue is discharged simultaneously, form oil phase;
3) aqueous solution that liquid-liquid separation obtains animal grease and collagen protein is carried out to formed oil phase and aqueous phase;
4) aqueous solution of obtained collagen protein is neutralized, desalination, filtration, concentrated, drying treatment, obtain collagen protein powder.
Animal wherein can be the domestic animals such as pig, sheep, ox, horse, donkey, mule, also can be the domesticated animal such as the poultry such as chicken, duck, goose, pigeon, quail, ostrich or fox, otter, spotted deer, rabbit.Described fatty tissue comprises the various tissue be made up of reticular tissue that is fatty and that wrap up these fat such as leaf fat, five flowers, fat meat, lower abdomen fat meat.Hydrolysis can be made catalyzer with sodium hydroxide and carry out, and also can make catalyzer with stomach en-, trypsinase, neutral protease, papoid, bromeline or Collagenase and carry out.Can carry out in aqueous, also can carry out in ethanol.When carrying out under being hydrolyzed catalysis at sodium hydroxide, be animal adipose tissue at solid-liquid ratio: sodium hydroxide solution=1: 2 ~ 10: 1, pH >=12,0 ~ 80 DEG C, preferably 40 ~ 60 DEG C, 0.1 ~ 1.0MPa, condition under carry out.When be hydrolyzed carry out under the catalysis of stomach en-, trypsinase, neutral protease, papoid, bromeline or Collagenase time, carry out under the optimal pH of enzyme used, optimum temperuture, normal pressure.Solid-liquid ratio is animal adipose tissue: enzyme liquid=1: 2 ~ 100: 1.When hydrolysis is carried out in ethanol, be 0-75% (v/v) in the concentration of ethanol used, solid-liquid ratio is animal adipose tissue: ethanolic soln=1: 10 ~ 100: 1, pH >=12,0 ~ 80 DEG C, preferably 40 ~ 60 DEG C, 0.1 ~ 1.0MPa, condition under carry out.Liquid-liquid separation can be settlement separate or centrifugation.Desalination can be dialysis, column chromatography, also can be ultrafiltration, electrodialysis or reverse osmosis desalination.Be concentrated in 25 ~ 80 DEG C, preferably 40 ~ 60 DEG C, 0.01 ~ 1.0MPa, condition under carry out.Drying can be vacuum-drying, also can be spraying dry, at 30 ~ 120 DEG C, preferably 50 ~ 100 DEG C, 0.01 ~ 1.0MPa, condition under carry out.
Embodiment
Example below will illustrate working method of the present invention, but can not as limitation of the invention.
Example one
3000g pig show condition is added in leacher, add 1000ml by 2mol/L sodium hydroxide and concentration be 96% (v/v) aqueous ethanolic solution according to 1: 1 the mixed solvent that forms of ratio, 10h is soaked at 60 DEG C, pig show condition dissolves completely, layering, upper strata is lard, and lower floor is collagen liquid.
After upper strata lard collection, cooling, obtain 1680g white lard, after testing, indices reaches country-level lard standard.
Underlying collagenous protein liquid is collected, and is neutralized to pH=6.8, puts on Rotary Evaporators with hydrochloric acid, 60 DEG C, under 1kPa, recovered alcohol is to the volume 1500ml of remaining liq, loads in dialysis tubing flowing tap water dialysis 48h, and then put on Rotary Evaporators, 60 DEG C, be concentrated into 1000ml under 1kPa, sabot is put in vacuum drying oven, 60 DEG C, dry 24h under 1kPa, pulverize, obtain collagen protein powder 840g.After testing, protein content wherein reaches more than 90%, can sell as collagen protein.
Example two
1500g chicken fatty tissue is added in large beaker, adds 1000ml neutral protein enzyme aqueous solution (containing neutral protease 500,000 unit), at 50 DEG C, soak 12h, chicken fatty tissue dissolves completely, layering, upper strata is chicken fat, and lower floor is collagen liquid.
After upper strata chicken fat collection, cooling, obtain 1200g chicken fat, after testing, indices reaches national chicken fat standard.
Underlying collagenous protein liquid is collected, and puts on Rotary Evaporators, 60 DEG C, is concentrated into 300ml under 1kPa, and Direct spraying is dry, obtains collagen protein powder 50g.After testing, protein content wherein reaches more than 90%, can sell as collagen protein.
Example three
800g otter subcutaneous lipids is added in beaker, adds 100ml Papain enzyme aqueous solution (containing papoid 100,000 unit), at 40 DEG C, soak 8h, subcutaneus adipose tissue dissolves completely, layering, upper strata is otter oil, and lower floor is collagen liquid.
After upper strata otter oil collection, cooling, obtain 600g otter oil, after testing, indices reaches the otter oil standard of national regulation.
Underlying collagenous protein liquid is collected, and put on Rotary Evaporators, 60 DEG C, be concentrated into 180ml under 1kPa, sabot is put in vacuum drying oven, 60 DEG C, dries 24h under 1kPa, pulverizes, obtains collagen protein powder 40g.After testing, protein content wherein reaches more than 90%, can sell as collagen protein.
Claims (10)
1. in animal adipose tissue, extract the process for cleanly preparing of lipid and collagen protein simultaneously.This technique is made up of the following step:
1) animal adipose tissue mincer is rubbed;
2) reticular tissue in animal adipose tissue is hydrolyzed, the reticular tissue in animal adipose tissue is dissolved, form collagen aqueous solution, the fat in animal adipose tissue is discharged simultaneously, form oil phase;
3) aqueous solution that liquid-liquid separation obtains animal grease and collagen protein is carried out to formed oil phase and aqueous phase;
4) aqueous solution of obtained collagen protein is neutralized, desalination, filtration, concentrated, drying treatment, obtain collagen protein powder.
2. in animal adipose tissue as claimed in claim 1, extract the process for cleanly preparing of lipid and collagen protein simultaneously, it is characterized in that described animal can be the domestic animals such as pig, sheep, ox, horse, donkey, mule, also can be the domesticated animal such as the poultry such as chicken, duck, goose, pigeon, quail, ostrich or fox, otter, spotted deer, rabbit.Described fatty tissue comprises the various tissue be made up of reticular tissue that is fatty and that wrap up these fat such as leaf fat, five flowers, fat meat, lower abdomen fat meat.
3. in animal adipose tissue as claimed in claim 1, extract the process for cleanly preparing of lipid and collagen protein simultaneously, it is characterized in that described hydrolysis can be made catalyzer with sodium hydroxide and carry out, also can make catalyzer with stomach en-, trypsinase, neutral protease, papoid, bromeline or Collagenase and carry out.Can carry out in aqueous, also can carry out in ethanol.
4. in animal adipose tissue as claimed in claim 3, extract the process for cleanly preparing of lipid and collagen protein simultaneously, it is characterized in that when carrying out under the catalysis of described hydrolysis at sodium hydroxide, it is animal adipose tissue at solid-liquid ratio: sodium hydroxide solution=1: 2 ~ 10: 1, pH >=12,0 ~ 80 DEG C, preferably 40 ~ 60 DEG C, 0.1 ~ 1.0MPa, condition under carry out.
5. in animal adipose tissue as claimed in claim 3, extract the process for cleanly preparing of lipid and collagen protein simultaneously, it is characterized in that when described hydrolysis is carried out under the catalysis of stomach en-, trypsinase, neutral protease, papoid, bromeline or Collagenase, carry out under the optimal pH of enzyme used, optimum temperuture, normal pressure.Solid-liquid ratio is animal adipose tissue: enzyme liquid=1: 2 ~ 100: 1.
6. in animal adipose tissue as claimed in claim 3, extract the process for cleanly preparing of lipid and collagen protein simultaneously, it is characterized in that when described hydrolysis is carried out in ethanol, be 0-75% (v/v) in the concentration of ethanol used, solid-liquid ratio is animal adipose tissue: ethanolic soln=1: 10 ~ 100: 1, pH >=12,0 ~ 80 DEG C, preferably 40 ~ 60 DEG C, 0.1 ~ 1.0MPa, condition under carry out.
7. extract the process for cleanly preparing of lipid and collagen protein in animal adipose tissue as claimed in claim 1 simultaneously, it is characterized in that described liquid-liquid separation can be settlement separate or centrifugation.
8. extract the process for cleanly preparing of lipid and collagen protein in animal adipose tissue as claimed in claim 1, it is characterized in that described desalination can be dialysis, column chromatography, also can be ultrafiltration, electrodialysis or reverse osmosis desalination simultaneously.
9. extract the process for cleanly preparing of lipid and collagen protein in animal adipose tissue as claimed in claim 1 simultaneously, it is characterized in that described being concentrated in 25 ~ 80 DEG C, preferably 40 ~ 60 DEG C, 0.01 ~ 1.0MPa, condition under carry out.
10. extract the process for cleanly preparing of lipid and collagen protein in animal adipose tissue as claimed in claim 1, it is characterized in that described drying can be vacuum-drying, also can be spraying dry simultaneously, at 30 ~ 120 DEG C, preferably 50 ~ 100 DEG C, 0.01 ~ 1.0MPa, condition under carry out.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410834564.5A CN104531334A (en) | 2014-12-24 | 2014-12-24 | Clean production process for simultaneously extracting lipid and collagen from animal fat tissues |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410834564.5A CN104531334A (en) | 2014-12-24 | 2014-12-24 | Clean production process for simultaneously extracting lipid and collagen from animal fat tissues |
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| CN104531334A true CN104531334A (en) | 2015-04-22 |
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| CN201410834564.5A Pending CN104531334A (en) | 2014-12-24 | 2014-12-24 | Clean production process for simultaneously extracting lipid and collagen from animal fat tissues |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106135631A (en) * | 2016-07-12 | 2016-11-23 | 张克庆 | The waste residue water that a kind of steam refining Adeps Sus domestica produces extracts the extraction process of animal proteinum |
| CN107739654A (en) * | 2017-11-14 | 2018-02-27 | 苏洁 | A kind of method of low-temperature purifying method deer oil |
| CN107937373A (en) * | 2017-11-29 | 2018-04-20 | 龚俊勇 | Complex enzyme formulation and grease extraction for grease extraction |
| CN112485079A (en) * | 2020-11-02 | 2021-03-12 | 武汉金开瑞生物工程有限公司 | Adipose tissue protein extraction kit and extraction method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106135631A (en) * | 2016-07-12 | 2016-11-23 | 张克庆 | The waste residue water that a kind of steam refining Adeps Sus domestica produces extracts the extraction process of animal proteinum |
| CN107739654A (en) * | 2017-11-14 | 2018-02-27 | 苏洁 | A kind of method of low-temperature purifying method deer oil |
| CN107937373A (en) * | 2017-11-29 | 2018-04-20 | 龚俊勇 | Complex enzyme formulation and grease extraction for grease extraction |
| CN107937373B (en) * | 2017-11-29 | 2020-05-01 | 龚俊勇 | Complex enzyme preparation for oil extraction and oil extraction method |
| CN112485079A (en) * | 2020-11-02 | 2021-03-12 | 武汉金开瑞生物工程有限公司 | Adipose tissue protein extraction kit and extraction method |
| CN112485079B (en) * | 2020-11-02 | 2022-09-06 | 武汉金开瑞生物工程有限公司 | Adipose tissue protein extraction kit and extraction method |
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Application publication date: 20150422 |