CN104528930B - Method for degrading organic chromium in dirt water through microorganism immobilization method - Google Patents
Method for degrading organic chromium in dirt water through microorganism immobilization method Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
- C02F3/1205—Particular type of activated sludge processes
- C02F3/1226—Particular type of activated sludge processes comprising an absorbent material suspended in the mixed liquor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract
An adsorption method in the immobilization technology is adopted, activated carbon is adopted as a carrier to immobilize microorganisms, and therefore waste water with the organic chromium of different concentrations is treated, and finally a set of optimal running parameters is determined, so that the discharge amount of Cr6+ in the chromate waste water meets the national discharging standard. According to the experiment, characteristic study is carried out on the treatment amount, the removal rate, the continuous running condition and influence factors (temperature, the pH value, the microorganism immobilization concentration, stay time and the like) in the treatment process, and finally the optimal charge rate, pH value, treatment period and the like are determined.
Description
Technical field
The present invention relates in waste water organic chromium minimizing technology, organic in specially a kind of immobilized microorganisms degrading waste water
The method of chromium.
Background technology
Chromium is trace element needed by human, plays special role in the carbohydrate metabolism and lipid metabolism of human body.Chromium is with organic
It is the material beneficial to human body that form exists, and enters human body and can be absorbed by the body, human body is generally to the utilization rate of organic chromium
10～25%, organic chromium has certain content in wholefood and medicine.And free chromium is poisonous, Cr VI is to people
Body has carcinogenecity, exists after free Cr VI needs removing and could discharge in water.
Currently for the minimizing technology comparative maturity of chromium ion inorganic in waste water, but, there is often also in waste water
Organic chromium, during water body flow, organic chromium can be decomposed into inorganic chromium, discharges after needing to remove again.Organic chromium is gone
Except method is then rarely reported or records it is therefore necessary to be studied accordingly, remove the organic pollution of chromium in waste water, reach state
Family's emission request.
Content of the invention
It is an object of the invention to provide in a kind of waste water organic chromium minimizing technology, specifically adopt immobilized microorganisms.
The present invention adopts the following technical scheme that realization:
In a kind of immobilized microorganisms degrading waste water, the method for organic chromium, comprises the steps:
(1), the domestication of microorganism and fixation: take aerobic activated sludge from sewage treatment plant, in the activated sludge fetched
Add and press the nutritional solution that c:n:p=100:5:1 configures, and composition 110ml suspension (because activated sludge lies substantially in liquid, one
As in the case of, add 5～10ml nutritional solution in the activated sludge measure 100～105ml, the effect of nutritional solution is to maintain activity
Microorganism in mud is in existing state, adds a small amount of)；It is distributed in time adding in the suspension containing activated sludge
The organic chromium solution entering variable concentrations is tamed to microorganism, after domestication, adds 1g granular active carbon, to microorganism
Carry out absorption to fix, form fixation of microbe；
(2) in the waste water containing organic chromium, add fixation of microbe, the ph value of adjustment waste water to 2～3, static 100～
120min；Wherein, every 200ml, add 1g activated carbon, the suspension containing activated sludge in the waste water solution of chromium concn 50mg/l
110ml.
This method adopts the absorption method in immobilization technology, that is, be exactly to make microorganism (activity dirt using activated carbon for carrier
Mud) solidification, thus organic chrome waste water is processed to variable concentrations, finally determine one group of optimal operational factor, so that
Cr in chromate waste water6+Discharge capacity reach discharging standards.The method to the treating capacity in processing procedure, clearance, continuously
Ruuning situation, influence factor's (temperature, ph value, fixation of microbe concentration, time of staying etc.) carries out characteristic research, finally determines
Optimum charging ratio, ph value, process cycle etc..
The domestication of microorganism and fixation: from the north suburb, sewage treatment plant takes aerobic activated sludge；In the activated sludge fetched
Add the nutritional solution pressing c:n:p=100:5:1 configuration, wherein, c source can select glucose, and n source can be with selective chlorination ammonium, p
Source can select potassium dihydrogen phosphate etc..It is distributed in time adding the organic chromium solution of variable concentrations in activated sludge to micro- life
Thing is tamed；The domestication of microorganism is the incremental material of addition targeting environment in bacteria culture media or substrate, allows
Antibacterial gradually adapts to and relies on material or the substrate of targeting environment, thus reaching the effective ingredient improving or changing in environment；One
As be applied to environmental protection industry (epi), medical fermentation industry, food fermentation industry etc..After domestication after a while, add graininess
Activated carbon, carries out absorption and fixes, and timing takes a certain amount of fixation of microbe out to carry out disposal ability mensure, makes to microorganism
It processes the organic chromium water sample of same concentrations in same time, compares disposal ability, selects the of a relatively high fixation of disposal ability
Microorganism is tested.
In experimentation, organic moiety in microbial decomposition organic chromium:
cr-(cxhyoz)+02----cr+xco2+y/2h2o.
The present invention is reasonable in design, this utilize immobilized microorganisms degrading waste water in organic chromium method, simple and fast, quickly
Organic pollution of chromium in degrading waste water, reaches national emission request.
Specific embodiment
Below the specific embodiment of the present invention is described in detail.
First, experiment condition
(1), main agents
Conventional chemical reagent: (1+1) sulphuric acid, (1+1) phosphoric acid, hydrochloric acid (hcl), sodium hydroxide (naoh), acetone (ch3-
co-ch3), potassium dichromate (k2cr2o7), diphenylcarbazide (c13h14n4Etc. o) it is analysis pure.
Developer: weigh diphenylcarbazide 0.2g, be dissolved in 50ml acetone, be diluted with water to 100ml, shake up.Store in
Brown bottle, puts in refrigerator and preserves.
Chromium Standard Reserving Solution: weigh in 120 DEG C of dryings potassium dichromate (k of 2 hours2cr2o7, analyze pure) and 0.2829g, uses
Move in 1000ml volumetric flask after water dissolution, be diluted with water to graticule, shake up.cr6+Concentration is 100mg/l.
Chromium standard solution: draw 5.00ml chromium Standard Reserving Solution in 500ml volumetric flask, be diluted with water to graticule, shake
Even.cr6+Concentration is 1mg/l.
Simulated wastewater: weigh the potassium dichromate (k being dried 2 hours in an oven2cr2o7, analyze pure) and 0.1415g, plus
Edta, adjusts ph value, with moving in 1000ml volumetric flask after water dissolution, is diluted with water to graticule, shakes up.cr6+Concentration is 50mg/l.
(2), key instrument equipment
Table 1 major experimental instrument
2nd, the operating procedure of the present embodiment specifically divides three parts to carry out, specific as follows:
1st, waste water, the collection of mud and pretreatment；
2nd, the culture experiment of microorganism；
3rd, the microbial immobilized experiment processing waste water:
1), granular active carbon adsorbs the research of the organic chromium in waste water；
2), in activated sludge absorption waste water organic chromium research；
3), activated carbon fixation of microbe processes the research of organic chromium in waste water.
In the waste water experimental stage, single-factor variable laboratory method is adopted to the process of organic chromium, find each factor (temperature, ph
Value, activated sludge dosage, activated carbon dosage etc.) impact to treatment effect, thus testing out optimal treatment condition.
3rd, the mensure of organic chromium
Measure cr in raw wastewater and water outlet6+Using regulation diphenylcarbazide ratio in national water quality monitoring standard method of analysis
Color method measures.In an acidic solution, hexavalent chromium and hexichol carbon phthalein two hydrazine reaction generate aubergine compound, in wavelength
Carry out spectrophotometry, absorbance closes Beer law with the relation character of concentration, when measuring total chromium, must first use Gao Meng at 540nm
Trivalent chromium oxidation in water sample is become Cr VI by sour potassium.
4th, the drafting of Cr VI standard curve
1st, prepare chromium standard reserving solution
Weigh the potassium dichromate (k being dried 2 hours in 120 DEG C of baking ovens2cr2o7) 0.2829g, soluble in water, then move into
In 1000ml volumetric flask, it is diluted with water to graticule, shake up.Every milliliter of hexavalent chromium containing 0.10mg of this solution.
2nd, prepare chromium standard solution
Prepare the volumetric flask of what a 500ml, take out the standard inventory of 5.00ml from chromium standard reserving solution with pipet
Liquid is put in the volumetric flask of 500ml, with distilled water diluting to graticule, shakes up.At this moment prepared is exactly every milliliter containing 1.00 μ g
Hexavalent chromium solution.But here it is mentioned that this solution will prepared using the same day.
(1), take 9 50ml color comparison tubes, sequentially add 0.00ml, 0.20ml, 0.50ml, 1.00ml, 2.00ml,
4.00ml, 6.00ml, 8.00ml, 10.00ml chromium standard solution, is diluted with water to graticule, plus 0.5ml sulfuric acid solution and 0.5ml
Phosphoric acid solution shakes up, and adds 2ml developer, shakes up.
(2), after 5～10min, at 540nm wavelength, with the cuvette of 10mm, with water as reference, mensuration absorbance and make
Blank correction.
(3), measurement result such as table 2 below
Table 2 hexavalent chromium concentration and absorbance data table
(4), the data in upper table is processed, deducted the absorbance drawing in blank assay, in the form of broken line graph
Draw the standard curve of chromic concentration and absorbance.With chromic concentration (mg/l) as abscissa, with variable concentrations
Corresponding absorbance (a) is vertical coordinate, prepared canonical plotting.
5th, chromic determination step
The present embodiment to measure six using the standard determination method in National Standard of the People's Republic of China (gb7467-87)
The content of valency chromium.In national standard method, the principle of determination of hexavalent chromium is: in an acidic solution, Cr VI and diphenylcarbazide
Reaction generates aubergine compound, can carry out the mensure of spectrophotometric value at wavelength 540nm using spectrophotometer.
(1), prepare the color comparison tube of a 50ml, take a certain amount of chromate waste water water sample, add in the color comparison tube of 50ml；
(2), it is separately added into (1+l) sulfuric acid solution 0.5ml preparing and (1+l) phosphoric acid solution 0.5ml, shake manually
Even；
(3), pipette the diphenylcarbazide developer of 2ml, be settled to 50ml, shake up manually；
(4), it is put into standing on test tube rack to be developed the color, after 5～10min, at 540nm wavelength, with the colorimetric of 10mm
Ware does reference, the numerical value of last mensuration absorbance with distilled water；
(5), data is processed, deducted the absorbance drawing in blank assay, the chromium standard in the upper section of correspondence is bent
Line reads chromic numerical value.
6th, experimental index and analysis of Influential Factors
(1) key index
The present embodiment is using the concentration of organic chromium in waste water as test index.
(2) influence factor
The factor that impact activated carbon immobilized microorganisms process waste water has a lot, including temperature, ph value, amounts of activated carbon, micro-
Other materials in biological concentration and activity and solution etc..In the present embodiment, in keeping solution, other materials are close, temperature
Under the conditions of identical, pay close attention to activated carbon dosage, activated sludge dosage, the shadow to processing waste water containing chrome performance for the ph value
Ring, so the influence factor of this experiment is ph, activated carbon, the dosage of microorganism.
(3) activated carbon immobilized microorganisms process the experimental result containing organic chrome waste water and analysis
1st, in activated carbon adsorption waste water organic chromium analysis of Influential Factors
(1) impact to adsorption effect for the ph
1) prepare organic chrome liquor of different ph
Water inlet ph value plays an important role in activated carbon adsorption chromate waste water, and generally it directly affects absorption effect
Really, and when ph value scope is different, the mechanism of its reaction and the form of product all differ widely.
Organic chrome liquor that different ph will be prepared in practical operation is extremely difficult, and also may be used by adding different amounts of acid solution
To control the ph of solution, do so is simpler, workable.According to the mass fraction of concentrated hydrochloric acid, density and will preparing
The ph value of solution, after 1ml concentrated hydrochloric acid dilutes 1000 times, recording its ph value with accurate ph reagent paper is 2.So according to this experience Lai
Add different acid solutions.
Weigh the potassium dichromate of 6 parts of 0.1415g with electronic balance, in beaker dissolve after, be separately added into 0.2ml,
The concentrated hydrochloric acid solution of 0.4ml, 0.6ml, 0.8ml, 1ml, 1.2ml and the edta of 10ml, 0.2mol/l, 1000ml's after stirring evenly
Constant volume in volumetric flask, the chrome liquor of as different ph.
2) activated carbon and reaction are weighed
Take 6 250ml beakers equipped with 1g activated carbon, be separately added into different ph values and contain organic chrome waste water 200ml, vibration
1h, carries out static adsorption.
3) survey absorbance
Reaction terminate after, take 7 50ml color comparison tube deionized water clean, labelled, number consecutively be 1,2,3,
4th, 5,6, blank, the supernatant in beaker is poured into the graduation mark to 50ml in corresponding color comparison tube, sequentially adds (the 1 of 0.5ml
+ 1) sulfuric acid solution and (1+1) phosphoric acid solution, shakes and all adds the developer of 2ml to shake all afterwards, use visible spectrophotometric after 5～10min
Measure its absorbance.
4) result and data analysiss
After the absorbance of solution in each color comparison tube is measured, the dense of its corresponding chromium is found out on standard curve
Degree, as aqueous concentration, calculate the clearance of organic chromium further according to influent concentration and aqueous concentration, acquired results are shown in Table 3.
The impact to adsorption effect for the table 3 ph value
Data analysiss: when waste water acid solution dosage is more than 0.6ml, activated carbon is to cr6+Clearance is up to 99. more than 6%.This
Mainly due under stronger acid condition, there are a large amount of cr containing in organic chrome waste water2o7 2-, these anion energy and activated carbon
Active adsorption composition form relatively stable compound, cr6+It is adsorbed on the activated carbon.
Waste water ph < when 2, cr6+Mainly it is reduced to cr3+, activated carbon is to cr3+Absorbability be much smaller than to cr6+Suction
Attached, it is unfavorable for the adsorption treatment of activated carbon；And waste water acidity is high, feedwater body causes acid pollution.Therefore, logical in actual treatment
Often adjusting waste water ph is 4 about.
(2) impact to adsorption effect for the activated carbon dosage
The organic chromium solution 200ml measuring 50mg/l respectively adjusts waste water ph=4, controls activated carbon dosage to be respectively 1g,
2g, 3g, 4g, 5g, 6g, 7g, 8g vibrate 1h, carry out static adsorption, with the increase of activated carbon dosage, containing going of organic chrome waste water
Except rate rises.When activated carbon dosage is 3g, organic chromium clearance is up to 99.3%.But activated carbon dosage increases to 5g organic chromium
Clearance only improves about 0.5%, and treatment effect does not have notable difference.Therefore, process 50ml concentration 200mg/l to give up containing organic chromium
During water, the optimum amount of activated carbon is 3g, and organic chromium content is 10mg organic chromium/3g activated carbon with the optimum ratio of activated carbon.
(3) adsorb the impact to adsorption effect for the time of contact
Adjusting waste water ph value is 4, and activated carbon dosage controls activated carbon with the time of contact containing organic chrome waste water respectively for 3g
For 0.25h, 0.5h, 1h, 2h, 3h, 4h, 5h, 6h, 7h carry out static adsorption, with the prolongation of absorption time of contact, organic chromium
Clearance tends to raising；During absorption 1h time of contact, the clearance of organic chromium is 99.53%.Continue to extend and adsorb time of contact,
Clearance tends towards stability.Therefore, activated carbon just processes organic chrome waste water optimal adsorption time of contact is 1h.
2nd, in activated sludge absorption waste water organic chromium research
(1) active sludge microorganism body acts on to the assimilation of soluble metal ion
It is an active absorption process that microorganism acts on to the assimilation of part heavy metal ion.By pure culture antibacterial pair
The result of study of metal ion assimilation shows, this process mainly includes two stages: be first one relatively rapid, need not
The passive adsorption process of energy expenditure, you can soluble metal ions are adsorbed to rapidly negatively charged microbial cell surface；
Then again cell wall is lentamente passed through by the active absorption process of microbial cell and cytoplasma membrane enters cell interior.Its
In, active absorption process is considered as restricted process in the process.
(2) in activated sludge absorption waste water organic chromium analysis of Influential Factors
1) ph value goes the impact of organic chromium in eliminating water to activated sludge
Weigh the potassium dichromate of 6 parts of 0.1415g with electronic balance, in beaker dissolve after, be separately added into 0.2ml,
The concentrated hydrochloric acid solution of 0.4ml, 0.6ml, 0.8ml, 1ml, 1.2ml, the edta of 10ml, 0.2mol/l, 1000ml's after stirring evenly
Constant volume in volumetric flask, the chrome liquor of as different ph.Static adsorption 90min under room temperature, standing, take supernatant diphenylcarbazide
Spectrophotometry absorbance, calculates the content of organic chromium, determines clearance.
Table 4 ph value goes the impact of chromium in eliminating water to activated sludge
Data analysiss: activated sludge absorption organic chromium is affected larger by solution ph.When ph value is for 1.0～10.0,
To chromium, adsorbance (vi) significantly rises activated sludge with the decline of ph value.When ph is for highly acid, with cr in solution2o7 -Account for
Advantage；Ph value for during neutrality mainly with hcro4And cro4 2-Two kinds of forms are removed.Because oh is contained on mud adsorbent surface,
The functional groups such as nh are protonated in low ph value, are conducive to cr2o2-Absorption.So activated sludge is to chromium (vi) in low ph value
There is higher clearance, suitable ph value is 1.0～2.0.
2) time of staying goes the impact of chromium in eliminating water to activated sludge
Measure the organic chromium solution 200ml of 50mg/l respectively, adjust ph value and be equal to 1.0～2.0, add equivalent amount of active mud
100ml add respectively in containing in organic chrome waste water, adsorb respectively under room temperature 10min, 20min, 30min, 40min, 50min,
60min, 90min, 120min, 150min, standing, take supernatant diphenyl carbazide spectrophotometry mensuration absorbance, meter
Calculate the content of organic chromium, determine clearance.
Data analysiss: this activated sludge increases with the prolongation in response time to the clearance of organic chromium.In absorption
Increase very fast, cr(vi after 120min within 120min) clearance reach 99.65%, then extend the time of staying, the increasing of clearance
Long very slow, and after absorption, mud shows good settling property.Showing that active sludge treatment contains organic chrome waste water is one
Individual quick adsorption reaction process, Best Times are 120min, extend process time too not big to organic chromium mass concentration
Change.Tests below chooses most optimal retention time 120min.
3) activated sludge consumption goes the impact of organic chromium in eliminating water to activated sludge
Measure the organic chromium solution 200ml of 50mg/l respectively, hydrochloric acid and sodium hydroxide solution regulation with debita spissitudo are molten
The ph value of liquid is 1.Excess sludge is taken to add respectively in containing in organic chrome waste water, dosage is respectively 50ml, 100ml, 150ml,
200ml, 250ml, 300ml, static adsorption 120min under room temperature, standing, take supernatant diphenyl carbazide spectrophotometry
Mensuration absorbance, calculates the content of organic chromium, determines clearance.
Data analysiss: with activated sludge consumption increase clearance in rising trend, dosage reach 260ml about when, go
Change except rate tends to be steady, and amplification will not be too obvious.
3rd, activated carbon immobilized microorganisms process the experimental result containing organic chrome waste water and analysis
(1) determination of optimization process effect
Measure the organic chromium solution 200ml of 50mg/l respectively, add 1g activated carbon, sequentially add 30ml, 60ml, 90ml,
120ml, 150ml, 180ml, 210ml activated sludge, static adsorption 120min under room temperature, standing, take supernatant hexichol carbon phthalein
Two trap spectrophotometry absorbances, calculate the content of organic chromium, determine clearance.When to determine amounts of activated carbon be 1g,
Good activated sludge dosage 110ml.
(2) ph value goes the impact of organic chromium in eliminating water to activated carbon fixation of microbe
Weigh the potassium dichromate of 6 parts of 0.1415g with electronic balance, in beaker dissolve after, be separately added into 0.2ml,
The concentrated hydrochloric acid solution of 0.4ml, 0.6ml, 0.8ml, 1ml, 1.2ml, the edta of 10ml, 0.2mol/l, 1000ml's after stirring evenly
Constant volume in volumetric flask, organic chrome liquor of as different ph.Measure organic chrome liquor 200ml respectively, addition 1g activated carbon is fixed
110ml activated sludge, static adsorption 120min under room temperature, standing, take supernatant hexichol carbon phthalein two hydrazine spectrophotometry
Absorbance, calculates the content of organic chromium, determines clearance.
Table 5 ph value goes the impact of organic chromium in eliminating water to activated carbon fixation of microbe
Data analysiss: with stepping up of sour dosage, clearance presents first increases the trend reducing afterwards, can by experience
Know sour dosage be 1.0ml when ph value for 2 so going in eliminating water to activated carbon fixation of microbe when can estimate ph 3 about
The treatment effect of organic chromium is optimal.
(3) dwell time values go the impact of organic chromium in eliminating water to activated carbon fixation of microbe
Measure the cr(vi of 50mg/l respectively) solution 200ml, the edta of 10ml, 0.2mol/l, adjust ph value equal to 3, plus
Enter equivalent amount of active mud 110ml add respectively in containing in organic chrome waste water, adsorb respectively under room temperature 10min, 20min, 30min,
60min, 90min, 120min, 150min, 300min, 600min, standing, take supernatant hexichol carbon phthalein two hydrazine spectrophotography
Mensuration absorbance, calculate cr(vi) content, determine clearance.
Data analysiss: this method increases with the prolongation in response time to the clearance of organic chromium.Within absorption 100min
Increase very fast, after 100min, the clearance of organic chromium reaches 95%, then extends the time of staying, and the growth of clearance is very slow, and
After absorption, mud shows good settling property.Best Times are 100min, extend process time to organic chromium quality
Concentration does not have too big change.
7th, conclusion
For the process containing organic chrome waste water, comprehensively examine Physical, chemical method, physical-chemical process and bioanalysises first
Conventional processing method, and to various method pluses and minuses summarize on the basis of it is proposed that activated carbon immobilized microorganisms process
Organic chromium in waste water.By the organic chromium in the organic chromium in activated carbon adsorption waste water, active sludge treatment waste water, activated carbon
The comparative study of chromium in fixation of microbe process waste water and the analysis of respective influence factor, are concluded that this enforcement
Activated sludge in example refers to add the aerobic activated sludge of nutritional solution.
(1) in activated carbon adsorption waste water, qualitatively analyze each factor (including ph value, amounts of activated carbon and the time of staying)
Impact to experimental result, so that it is determined that processing 200ml, the optimal ph value of the organic chromium solution of 50mg/l is 4, amounts of activated carbon 3g
With time of staying 1h.
(2) in the research process of the organic chromium in active sludge treatment waste water, qualitatively analyze each factor (include ph
Value, amounts of activated carbon and the time of staying) impact to experimental result, so that it is determined that processing 200ml, the organic chromium solution of 50mg/l
Optimal ph value is 1～2, active mud content 260ml and time of staying 2h.
(3), during, being determined by experiment out the organic chromium that activated carbon fixation of microbe is processed in waste water, determination is processed
When the optimum activity charcoal amount of the organic chromium solution of 200ml, 50mg/l is 1g, optimum activity mud dosage 110ml(contains nutritious
The aerobic activated sludge of liquid).Pass through experiment according to such ratio and equally draw optimization process ph value 3, time of staying 100min.
(4), contrast finds that the organic chromium that activated carbon immobilized microorganisms are processed in waste water has practical feasibility, processes effect
Fruit reaches more than 95%, reaches national wastewater discharge standard, and has the characteristics that oneself with respect to conventional chemical methods, activated carbon is fixed
Microbial method processes high concentration and contains organic chrome waste water, and technological process is simple, is easy to control management；System has stronger impact resistance
The ability of load；The processing cost that unit chromium removes is relatively low.
Claims (1)
1. in a kind of immobilized microorganisms degrading waste water organic chromium method it is characterised in that: comprise the steps:
(1), the domestication of microorganism and fixation: take aerobic activated sludge from sewage treatment plant, add in the activated sludge fetched
By the nutritional solution of c:n:p=100:5:1 configuration, constitute 110ml suspension；Wherein, suspension is by the activity measuring 100～105ml
5～10ml nutritional solution is added to constitute in mud；
It is distributed in time adding the organic chromium solution of variable concentrations that microorganism is tamed and dociled in the suspension containing activated sludge
Change, after domestication, add 1g granular active carbon, microorganism is carried out with absorption and fixes, form fixation of microbe；
(2) in the waste water containing organic chromium, add fixation of microbe, the ph value of adjustment waste water to 3, static 100～120min；
Wherein, every 200ml, add 1g activated carbon, the suspension 110ml containing activated sludge in the waste water solution of chromium concn 50mg/l.
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| CN111423076A (en) * | 2020-05-23 | 2020-07-17 | 樊晓瑜 | Landfill leachate treatment system |
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| CN113149230B (en) * | 2021-02-26 | 2022-04-19 | 中南大学 | Treatment method for reducing hexavalent chromium by wood charcoal electron transfer enhanced microorganisms |
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|---|---|---|---|---|
| US4033763A (en) * | 1975-10-15 | 1977-07-05 | World Resources Company | Process for recovery of selected metal values from waste waters |
| PT103332A (en) * | 2005-08-12 | 2007-02-28 | Univ Do Minho | BIOSAFETY SYSTEM PRODUCED FROM BIOFILMS SUPPORTED IN FAUJASITE ZEÓLITO (FAU), PROCESS FOR OBTAINING AND USING IT IN THE REMOVAL OF HEXAVALENT CHROMIUM (CR (VI)) |
| CN101676224A (en) * | 2008-09-18 | 2010-03-24 | 中国科学院福建物质结构研究所 | Method for treating industrial chromium-containing waste water by bacteria |
| CN101712506B (en) * | 2008-10-08 | 2013-02-13 | 中国科学院城市环境研究所 | Method for reducing hexavalent chromium in water by biological waste |
| CN101402491A (en) * | 2008-10-27 | 2009-04-08 | 合肥工业大学 | Method for treating heavy metal-containing acidic waste water and recycling heavy metal with biomass fixed bed |
| CN103910437B (en) * | 2014-04-18 | 2015-07-15 | 湖南大学 | Method for removing heavy metal ions out of water |
| CN103908949A (en) * | 2014-04-25 | 2014-07-09 | 湖南城市学院 | Sludge based activated carbon and production method thereof, and application of sludge based activated carbon in electroplating of chromate-containing waste water |
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