CN104523768A - 细叶云南松松针精油的应用 - Google Patents

细叶云南松松针精油的应用 Download PDF

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CN104523768A
CN104523768A CN201410763069.XA CN201410763069A CN104523768A CN 104523768 A CN104523768 A CN 104523768A CN 201410763069 A CN201410763069 A CN 201410763069A CN 104523768 A CN104523768 A CN 104523768A
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essential oil
pinus yunnanensis
pine needles
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pine needle
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田玉红
李梓
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Guangxi University of Science and Technology
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Guangxi University of Science and Technology
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/13—Coniferophyta (gymnosperms)
    • A61K36/15—Pinaceae (Pine family), e.g. pine or cedar
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
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  • Alternative & Traditional Medicine (AREA)
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  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
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  • Veterinary Medicine (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

本发明公开一种细叶云南松松针精油的应用，将所述松针精油作为大肠杆菌抑制剂。与其它松的松针精油相比，本发明的细叶云南松松针精油对大肠杆菌具有良好的抑制效果。

Description

细叶云南松松针精油的应用
技术领域
本发明涉及细叶云南松松针精油的应用。
背景技术
松针药用历史悠久，具有止痒、明目安神、祛风活血、解毒等功效，用于治疗冻疮、流行性感冒、跌打肿痛、风湿性关节炎等。现代药理学研究表明，松针具有抑菌、降血压、降血脂、镇痛、抗突变、祛痰、抗感染等作用。松针中含有钙、钾、铁等无机盐和维生素A、维生素C，19种脂肪酸，17种氨基酸等40余种营养成分，而且还含有挥发油类、木脂素类、色素、黄酮类。因此松针的开发利用价值不可忽视。
发明内容
本发明要解决的技术问题是克服现有的缺陷，提供了一种细叶云南松松针精油的应用。
为了解决上述技术问题，本发明提供了如下的技术方案：
细叶云南松松针精油的应用，所述松针精油作为大肠杆菌抑制剂。
所述松针精油通过如下步骤得到：将松针切碎置于挥发油提取器中，按料液为1：4～1：20加入水，浸泡4-16小时，然后加热回流提取1-2小时。
进一步，料液比为1：12，浸泡4小时，回流提取2小时。
具体实施方式
以下对本发明的优选实施例进行说明，应当理解，此处所描述的优选实施例仅用于说明和解释本发明，并不用于限定本发明。
实施例1
称取100g阴干的细叶云南松松针，将针叶切成长度2cm左右，把切碎的松针置于挥发油提取器中，按料液比为1:12加入水，浸泡4h后，加热回流提取精油，提取时间为2h，提取完毕后读取精油的体积，并平行试验三次，提取率为0.62（mL·100g-1，提取率以干重计）。
实施例2
称取100g阴干的细叶云南松松针，将针叶切成长度2cm左右，把切碎的松针置于挥发油提取器中，按料液比为1:10加入水，浸泡4h后，加热回流提取精油，提取时间为0.5h，提取完毕后读取精油的体积，并平行试验三次，提取率为0.46（mL·100g-1，提取率以干重计）。
实施例3
称取100g阴干的细叶云南松松针，将针叶切成长度2cm左右，把切碎的松针置于挥发油提取器中，按料液比为1:15加入水，浸泡12h后，加热回流提取精油，提取时间为2h，提取完毕后读取精油的体积，并平行试验三次，提取率为0.44（mL·100g-1，提取率以干重计）。
抑菌活性测试
采用滤纸片扩散法测定拉雅松和细叶云南松松针精油（均按实施例1制得）的抑菌活性，以阿莫西林颗粒（对细菌）和克霉唑（对霉菌）作为阳性对照。具体实验过程如下：在标准净化台上，用无菌移液器吸取0.2mL的测试菌悬液，将其均匀涂布于固体培养基上，正置于培养箱1h（细菌于37℃恒温培养，真菌于28℃恒温培养）。1h后移于净化台中，将直径6mm的无菌双层滤纸片放置于培养皿中心，然后用无菌移液器分别移取10μL的待测样品（精油和阳性对照）垂直加到滤纸片上，培养皿先正置培养1h，然后将含细菌菌株的培养皿在37℃下倒置恒温培养24h，含真菌菌株的培养皿在28℃下倒置恒温培养48h。测量抑菌圈的直径，以此衡量松针精油的抑菌效果。
拉雅松和细叶云南松松针精油的抑菌活性
附注：a：NA没有效果；b：NT没有测试
细菌：大肠杆菌（Escherichia coli）、沙门氏菌（Salmonella sp.）、枯草芽孢杆菌（Bacillus subtilis）、金黄色葡萄球菌（Staphylococus aureus）。
霉菌：黑曲霉（Aspergillus niger）、橘青霉（Penicillium citrinum.）、根霉（Rhizopus sp.）、黄曲霉（Aspergillus flavus）。
酵母：热带假丝酵母（Candida tropicalis）、红酵母（Rhodotorula sp.）、酿酒酵母（Saccharomyces cerevisiae）。
如上可以看出，与拉雅松松针精油对大肠杆菌没有抑制效果相比，细叶云南松松针精油对大肠杆菌具有良好的抑制效果。
最后应说明的是：以上所述仅为本发明的优选实施例而已，并不用于限制本发明，尽管参照前述实施例对本发明进行了详细的说明，对于本领域的技术人员来说，其依然可以对前述各实施例所记载的技术方案进行修改，或者对其中部分技术特征进行等同替换。凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。

Claims (3)

1.细叶云南松松针精油的应用，所述松针精油作为大肠杆菌抑制剂。
2.根据权利要求1所述细叶云南松松针精油的应用，其特征在于，所述松针精油通过如下步骤得到：
将松针切碎置于挥发油提取器中，按料液为1：4～1：20加入水，浸泡4-16小时，然后加热回流提取1-2小时。
3.根据权利要求2所述细叶云南松松针精油的应用，其特征在于：所述料液比为1：12，浸泡4小时，回流提取2小时。
CN201410763069.XA 2014-12-12 2014-12-12 细叶云南松松针精油的应用 Pending CN104523768A (zh)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109576061A (zh) * 2019-01-21 2019-04-05 黑龙江省林业科学研究所 一种红松松针精油及其提取方法和在抑菌培养基中的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103385262A (zh) * 2012-05-11 2013-11-13 苏州纳特苏生物科技有限公司 一种驱除蚊虫的复方精油

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103385262A (zh) * 2012-05-11 2013-11-13 苏州纳特苏生物科技有限公司 一种驱除蚊虫的复方精油

Non-Patent Citations (2)

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Title
文福姬等: "松树精油的化学成分及抗菌活性研究", 《林业实用技术》 *
李梓: "拉雅松和细叶云南松挥发性成分的提取及生物活性研究", 《中国优秀硕士学位论文全文数据库工程科技Ⅰ辑》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109576061A (zh) * 2019-01-21 2019-04-05 黑龙江省林业科学研究所 一种红松松针精油及其提取方法和在抑菌培养基中的应用

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