CN104523341A - Method for manufacturing immediately implanted tooth with periodontal bioactivity - Google Patents
Method for manufacturing immediately implanted tooth with periodontal bioactivity Download PDFInfo
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- CN104523341A CN104523341A CN201410781591.0A CN201410781591A CN104523341A CN 104523341 A CN104523341 A CN 104523341A CN 201410781591 A CN201410781591 A CN 201410781591A CN 104523341 A CN104523341 A CN 104523341A
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Abstract
The invention relates to a method for manufacturing an immediately implanted tooth with periodontal bioactivity. The method comprises the following steps: (1) preparing a pure titanium or titanium alloy implant core; (2) preparing self-curing calcium-magnesium phosphate powder; (3) preparing a collagen solution; (4) preparing an artificially synthesized P substance polypeptide; (5) preparing an SP crosslinking collagen substance; (6) mixing and stirring the calcium-magnesium phosphate powder and the SP crosslinking collagen substrate to prepare bioactive paste; (7) coating the root part of the implant core with the bioactive paste, pressurizing, curing and lyophilizing to prepare an artificial tooth root with periodontal bioactivity; (8) connecting the crown part of the implant core to an artificial tooth crown suitable for the tooth-missing appearance to obtain the immediately implanted tooth. According to the method, the artificial tooth root with a bionic cementum structure is constructed, so that the prepared implant tooth has partial life signs of a natural transplanted tooth, has spontaneous periodontal curing bioactivity, can be immediately implanted after a tooth is extracted, and can be used for remarkably shortening the tooth repairing time and improving the implant success rate.
Description
Technical field
The invention belongs to artificial dental implantation body technical field, relate to a kind of manufacture method of biological activity Dental Implant, particularly relate to a kind of can after exodontia immediate implant and initiatively induction between the root of the tooth and tooth socket osseous tissue of Dental Implant, form synestotic Dental Implant manufacture method.
Background technology
Dental Implant (dental implant) is called as the 3rd secondary tooth of the mankind, has become modern stomatologic significant technology.Modern dentistry planting technology full-fledged is the synosteosis theory (osseointegration, or be called Integrated implant) proposed the sixties in last century based on the father Branemark of Dental Implant.American Dental implantology can be defined as synestotic: normal reconstruction bone directly contacts with implantation body, grow into, and the based model for load duration of Neng Shi implantation body conducts and is dispersed in osseous tissue without the soft tissue can seen under light microscopic.
Modern tooth implant mainly adopts the inorganic material such as titanium or pottery to be made by machining, material surface has good biocompatibility, but be difficult to build continual and steady regeneration inducing bioactivity, so the quick healing after implantation body's implantation needs good primary stability, that is, during implantation, implantation body just can form the direct of enough areas with surrounding socket bone and contacts., carry out when the healing of 3-6 crescent nest osteanagenesis is good after traditional implant operation is generally arranged in exodontia for this reason, and need after implanting to embed or static healing 3 months, to obtain good Osseointegrated implants, then row second operation.Connect top dentures structure.
Immediate implant, is Dental Implant is implanted tooth socket in exodontia simultaneously, can connects dentures repai agomphosis, thus reduce operation number of times and operation wound, early recovery dental articulation and Aesthetic Function after its healing, is the important directions of oral cavity reconstruction technique development.After research in recent years shows to have tooth pulled out, immediate implant also can realize synosteosis healing, but, form and the implantation body of fresh tooth socket are difficult to mate completely, and alveolar bone may be impaired because of periodontal and tip of a root disease etc., thus immediate implant is usually difficult to obtain necessary primary stability and good healing environment, and its nearly long-term function and aesthetic effect still have more arguement.Current immediate implant is suitable for site and is generally labial teeth and premolars, rooting side is wanted to have more than 3-5mm bone amount at least, with stable Implant, and tooth socket is roomy after site exodontia of grinding one's teeth in sleep, interalveolar septum bone amount is difficult to support dental implants stability more, so clinical little employing immediate implant, generally exodontia after 4 months until tooth socket complete bony union time plant.If suffer from tooth site to there is a large amount of Cranial defect and/or soft tissue inflammation because of parodontopathy and/or periapical periodontitis, or patient has the whole body system such as osteoporosis, diabetes disease, neither the indication of immediate implant.In addition, labial teeth district lip buccal side alveolar bone wall forms primarily of the cortical bone of weakness, and tooth physiologic resorption can occur after pulling out thereupon, and existing implantation body can not stop this bone resorption, gingival recession and implantation body edge may be caused to expose, have a strong impact on aesthetic effect.In sum, when current implantation body is designed for immediate implant, need more strict Candidacy selection, and general more complicated material and the technology of needing is assisted, clinical practice is subject to more restriction.
Utilize bioactie agent to load, make implantation body possess the ability of bone induction and regeneration reconstruction, improving feasibility and the success rate of immediate implant, is the important directions of planting technology and theoretical developments.Conventional osteanagenesis relevant growth factors or hormone are as BMP, parathyroid hormone etc. at present, by various physics or chemical method, build biological activity modify top layer in implant surface.But these somatomedin are high molecular weight protein, more problem is all there is in acquisition synthesis, location conveying and activity control etc., particularly in implantation body's surface of metal titanium, how to load organic biomolecules, how to stablize and keep its biological activity, and how to ensure its biological safety, also have more technical bottleneck, distance dentistry plantation clinical practice application conditions still has larger gap.
Summary of the invention
The object of this invention is to provide a kind of manufacture method with the bioactive immediate implantation teeth of periodontal, the Dental Implant prepared by the method has the artificial tooth root of bionical natural teeth root of the tooth cementum structure, possesses autonomous bone induction and regeneration reconstruction ability, can in the rear immediate implant of exodontia, simulate natural planted teeth periodontal healing process, solve the problem that agomphosis Complicated primary is rebuild.
In order to achieve the above object, the invention provides following technical scheme: a kind of manufacture method with the bioactive immediate implantation teeth of periodontal, it comprises the following steps: (1) prepares pure titanium or titanium alloy implant core, wherein, described implantation body core comprises bizet and root, has screw in described bizet, for fixing artificial corona, the outer surface of described root has screw thread, for disperseing bite stress, (2) prepare the agent of self-curable calcium phosphate magnesium powder, concrete grammar is: according to the mol ratio of Mg: Ca=3: 1, by Mg (OH)
2with CaCO
3mixing, MgHPO
43H
2o and CaHPO
4mixing, then by two kinds of mixture according to the mixed in molar ratio of 1: 2, and then mixed material is sintered 5 hours at 1100 degrees celsius, prepare calcium phosphate magnesium material Mg
2.25ca
0.75(PO
4)
2, material Mg is burnt by mortar broken gained calcium phosphate magnesium
2.25ca
0.75(PO
4)
2, and then to sieve by 355 μm of aperture sieve, after screening, within 10 minutes, make powder with ball mill grinding again, and by described powder according to 1: 1 mol ratio and Ca (H
2pO
4)
2h
2o mix and blend 30 seconds, makes the agent of self-curable calcium phosphate magnesium powder, (3) collagen solution is prepared, concrete grammar is: be that 3%W/V adds in the acetic acid solution of 5mol/L by I-type collagen fiber according to mass body volume concentrations, and 4 DEG C of temperature lower magnetic force stirring and dissolving, then solution semipermeable membrane distilled water is dialysed and remove acetic acid composition in 48 hours, make collagen solution, (4) prepare synthetic Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 polypeptide, concrete grammar is: use automatic Peptide synthesizer according to following aminoacid sequence Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 synthetic Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 polypeptide, (5) Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 covalent cross-linking modified collagen substrate is prepared, concrete grammar is: with 1-ethyl-(3, 3 '-dimethylaminopropyl) phosphinylidyne diimine and N-hydroxy-succinamide be covalent crosslinking agent, by described Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 polypeptide, 1-ethyl-(3, 3 '-dimethylaminopropyl) phosphinylidyne diimine and N-hydroxy-succinamide mix according to mol ratio 1: 1.2: 0.6, then be that 1%W/V adds in described collagen solution by mixture according to mass body volume concentrations, first magnetic agitation reacts 24 hours, then semipermeable membrane distilled water is dialysed and within 72 hours, is made Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 covalent cross-linking modified collagen substrate, (6) citric acid soln of the agent of described self-curable calcium phosphate magnesium powder and 0.5mol/L is adjusted to mix according to mass volume ratio example and is mixed and made into self-curable calcium phosphate magnesium paste for 3.0g: 1ml, then with volume ratio 60%: 40% described self-curable calcium phosphate magnesium paste and described Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 covalent cross-linking modified collagen substrate adjusted to mix and be mixed and made into biological activity paste, (7) two-part biological activity paste cure under pressure mould is prepared, epimere is used for holding and fixing described bizet (1), hypomere is for holding described root (2) and described biological activity paste, the surface of described root (2) is made to have the biological activity paste bag quilt of 3mm thickness, then by the split of described two-part biological activity paste pressurization fixed line mould together and be forced into 200-300kPa, be placed in 100% humidity environment and within 24 hours, make described calcium phosphate magnesium paste curing, freezing 12 hours in-80 DEG C again, vacuum lyophilization 48 hours, the collagen component in the crystallization of calcium phosphate magnesium is made to be solidified into dimensional fibre network-like structure, make the artificial tooth root with bionical natural teeth root of the tooth cementum structure, (8) prepare artificial corona (8), make its form and agomphosis plesiomorphism, be fixed on described bizet (1) by screw (7), namely can be made into immediate implantation teeth.
Further, wherein, the connecting portion of described bizet and described root has the step of 0.8-1mm.
Further, wherein, finally the thick bioactive layer of 3mm is formed at the outer surface of described root.
Superiority of the present invention is: adopt pure titanium or titanium alloys for tooth implant core, possess good biocompatibility, machining property, Mechanics of Machinery character, can provide the bulk strength of similar natural teeth for immediate implantation teeth; Bizet can adopt screw to connect the artificial corona of corresponding form according to agomphosis position, is suitable for the agomphosis reparation of different parts; Tooth root subpackage is by calcium phosphate magnesium-collagen composite bioactive materials, this composite bio-active material is based on calcium phosphate, be magnesium ion by thermal chemical reaction by calcium ion aliquot replacement, the osseous tissue of the Mechanics of Machinery character and improvement that obtain strengthening guides active, by modulation mixing Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 (SP) covalent cross-linking modified collagen, obtaining continual and steady polypeptide mobilizes stem cell biological active, by pressurization self-curing and lyophilization process, obtain the cemental 26S Proteasome Structure and Function of simulation natural teeth root of the tooth, make biomimetic prosthetic root of the tooth.This Dental Implant can after exodontia immediate implant, there is the periodontal biological activity of autonomous induction Regeneration of Alveolar Bone reconstruction, significantly can shorten agomphosis repair time, improve success rate.
Accompanying drawing explanation
Fig. 1 is the enlarged drawing with the bioactive immediate implantation teeth of periodontal adopting method manufacture of the present invention.
Detailed description of the invention
Describe the specific embodiment of the present invention in detail below in conjunction with accompanying drawing, the content of detailed description of the invention is not as the restriction to protection scope of the present invention.
The non-coating biology modification technology that the manufacture method with the bioactive immediate implantation teeth of periodontal of the present invention utilizes materialogy and organizational project to learn a skill to combine, build bionical cementum structure artificial root of the tooth, thus make prepare Dental Implant can after exodontia immediate implant, possesses the part vital signs of natural planted teeth, very big shortening agomphosis repair time, significantly improve plantation success rate.
Design of the present invention utilizes small-molecular peptides section to build biological activity, utilizes self-curable calcium phosphate material construction natural teeth cementum biomimetic features.Small active peptides has significant advantage in synthesis preservation, functional localization and security control etc., is build the bioactive better selection of organizational project.Research shows, 11 peptide Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 (Substance P, SP) marrow stromal cell, vascular endothelial cell, neutrophilic granulocyte, epithelial cell etc. are distributed widely in, have well-conserved, it is the neuroendocrine signal of mobilizing stem cell activation and differentiation, having induction of vascular new life, promote the several functions effect such as osteanagenesis and bone conversion, is a kind ofly conveniently to be easy to get, to act on various, safe and reliable small active peptides.And self-curable calcium phosphate is in structure composite biological material, there is unique advantage: calcium phosphate itself has osseous tissue and guides active, can be the hydroxyapatite crystal state with good strength from paste state self-curing that can be arbitrarily moulding, solidification intensity can be controlled by methods such as ion exchanges, curing reaction is gentle, bioactive ingredients can be loaded by the various ways such as physical absorption, covalent cross-linking, realize bone guided+self-bone grafting multi-biological active.
In the present invention, smooth turning lathe is first utilized to prepare pure titanium or titanium alloy tooth implant core.As shown in Figure 1, described pure titanium or titanium alloy implant core comprise bizet 1 and root 2, and the connecting portion of described bizet 1 and described root 2 has the step 3 of 0.8-1mm.The center of described bizet 1 has screwed hole 4, can fix artificial corona 8 by the screw 7 inserting described screwed hole 4.The outer surface of described root 2 has screw thread 5, disperses bite stress by described screw thread 5.
Second step, prepares the agent of self-curable calcium phosphate magnesium powder.Concrete grammar is: according to the mol ratio of Mg: Ca=3: 1, by Mg (OH)
2with CaCO
3mixing, MgHPO
43H
2o and CaHPO
4mixing, then by two kinds of mixture according to the mixed in molar ratio of 1: 2, and then mixed material is sintered 5 hours at 1100 degrees celsius, by thermal chemical reaction with magnesium ion Substitute For Partial calcium ion, prepare calcium phosphate magnesium material Mg
2.25ca
0.75(PO
4)
2.Then, by the calcium phosphate magnesium material Mg prepared by mortar fragmentation
2.25ca
0.75(PO
4)
2, and then to sieve by 355 μm of aperture sieve, after screening, within 10 minutes, make powder with ball mill grinding again, and by described powder according to 1: 1 mol ratio and Ca (H
2pO
4)
2h
2o mix and blend 30 seconds, makes the agent of self-curable calcium phosphate magnesium powder.
3rd step, prepares collagen solution.Concrete grammar is: acetic acid solution type i collagen fiber being added 5mol/L according to 3% (quality volume W/V) concentration, stir at 4 DEG C of temperature lower magnetic forces and fully dissolve for 24 hours, then obtained solution semipermeable membrane distilled water is dialysed 48 hours, within every 6 hours, change distilled water, remove acetic acid composition, make collagen solution.
4th step, prepares synthetic Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 polypeptide.Concrete grammar is: use automatic Peptide synthesizer according to following aminoacid sequence Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 synthetic Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 polypeptide.
5th step, prepares Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 covalent cross-linking modified collagen substrate.Concrete grammar is: with 1-ethyl-(3,3 '-dimethylaminopropyl) phosphinylidyne diimine and N-hydroxy-succinamide be covalent crosslinking agent, by described Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 polypeptide, 1-ethyl-(3,3 '-dimethylaminopropyl) phosphinylidyne diimine and N-hydroxy-succinamide mix according to mol ratio 1: 1.2: 0.6, then be that 1%W/V adds in described collagen solution by mixture according to mass body volume concentrations, first magnetic agitation reacts 24 hours, and then semipermeable membrane distilled water is dialysed and within 72 hours, made Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 covalent cross-linking modified collagen substrate.
6th step, be that 3.0g/ml adjusts to mix and is mixed and made into self-curable calcium phosphate magnesium paste by the citric acid soln of the agent of described self-curable calcium phosphate magnesium powder and 0.5mol/L according to mass body volume concentrations, again with the ratio of 60%: 40% volume ratio, described self-curable calcium phosphate magnesium paste is adjusted to mix with described Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 covalent cross-linking modified collagen substrate and mixes, insert in curing mold, then the root 1 of described titanium implant core and part bizet 2 are inserted in described curing mold, and be forced into 200-300kPa, solidify 24 hours under 100% humidity, freezing 12 hours in-80 DEG C again, vacuum lyophilization 48 hours.Wherein, described curing mold than described tooth implant core root diameter (RD) and have more 3mm highly respectively, thus make the root 1 pan coating self-curable calcium phosphate magnesium-SP biological active collagen material of described tooth implant core, and then Dental Implant is made to mobilize stem cell biological activity and the bioactive immediate implantation teeth of simulation natural teeth periodontal healing for there being SP.
In the present invention, use the self-curing composite based on calcium phosphate, there is good material compatibility and histocompatibility, have the following advantages for artificial tooth root preparation: be easy to moulding processing (comprising integral material profile, internal stent structure and rack surface form etc.), material with adjustable intensity, controllable degradation rate, several functions loads (comprising as stem cell transplantation carrier, biotic factor/medicine loading and slow-released carrier etc.), has bone guided and bone-inducting active concurrently.For solution Dental Implant periodontal healing problem, there is unique advantage.
SP in the present invention is except participating in pain sensation conduction as neurotransmitter, also be distributed widely in marrow stromal cell, vascular endothelial cell, neutrophilic granulocyte, epithelial cell etc., play multiple effect, being considered to the system response courier of a wound, is the important molecule of mobilizing stem cell and promoting repair in trauma.SP also has the function of induction of vascular new life, and its mechanism may comprise direct induction of vascular endothelial cell differentiation and propagation, and indirectly raises activating vessels by TNF-a signal etc. and form other relevant cells.SP also plays an important role in osteanagenesis and bone conversion (turnover, bone resorption and bone formation) activity.In vitro study shows that SP can stimulate marrow stromal cell (bone marrow stromal cells, or BMSCs) proliferation and differentiation in concentration-dependent relation, pharmacological research display, SP does not have bad pharmacological action or genetoxic, can be safe mobilize and treatment for cells in vivo.Based on above multifrequency nature, SP to be a kind ofly conveniently easy to get, to act on various, safe and reliable small active peptides, has the good action being applied to artificial organ organizational project.
Simple SP polypeptide in vivo can fast degradation and losing activity, think realize SP body in biological effect, suitable delivery, location, slow-released system should be designed with the function of control SP.In the present invention, by covalent cross-linking molecule, SP is connected to collagen fiber, its major advantage is: be 1. similar to extracellular matrix, for cell provides nanoscale three-dimensional microenvironment, is conducive to sticking and growing of cell.2. collagen is degraded gradually, stable sustained-release SP, continues to produce stem cell mobilization effect.3. three-dimensional collagen fiber network is compound between the calcium phosphate magnesium crystallization of solidification, and simulation root of the tooth cementum structure, forms biomimetic prosthetic root of the tooth, and realize metaplasia with guiding+induction mode in vivo.
In the present invention, autonomous induction periodontal healing environment is built by artificial tooth root bioactive substance, can regenerate by inducing endogenous Periodontal Supporting Tissue after implanting, complete artificial material and reconstruct to the conversion of autologous tissue, and the periodontal attachment supporting structure that final formation is stable.
The content of detailed description of the invention is understood for the ease of those skilled in the art and uses the present invention and describe, and does not form restriction the present invention being protected to content.Those skilled in the art, after having read content of the present invention, can carry out suitable amendment to the present invention.Protection content of the present invention is as the criterion with the content of claim.When not departing from flesh and blood and the protection domain of claim, the various amendments carried out the present invention, change and replacement etc. are all within protection scope of the present invention.
Claims (3)
1. have a manufacture method for the bioactive immediate implantation teeth of periodontal, it comprises the following steps:
(1) pure titanium or titanium alloy implant core is prepared, wherein, described implantation body core comprises bizet (1) and root (2), screw (4) is had in described bizet (1), for fixing artificial corona (8), the outer surface of described root (2) has screw thread (5), for disperseing bite stress;
(2) prepare the agent of self-curable calcium phosphate magnesium powder, concrete grammar is: according to the mol ratio of Mg: Ca=3: 1, by Mg (OH)
2with CaCO
3mixing, MgHPO
43H
2o and CaHPO
4mixing, then by two kinds of mixture according to the mixed in molar ratio of 1: 2, and then mixed material is sintered 5 hours at 1100 degrees celsius, prepare calcium phosphate magnesium material Mg
2.25ca
0.75(PO
4)
2; Material Mg is burnt by mortar broken gained calcium phosphate magnesium
2.25ca
0.75(PO
4)
2, and then to sieve by 355 μm of aperture sieve, after screening, within 10 minutes, make powder with ball mill grinding again, and by described powder according to 1: 1 mol ratio and Ca (H
2pO
4)
2h
2o mix and blend 30 seconds, makes the agent of self-curable calcium phosphate magnesium powder;
(3) collagen solution is prepared, concrete grammar is: be that 3%W/V adds in the acetic acid solution of 5mol/L by I-type collagen fiber according to mass body volume concentrations, and 4 DEG C of temperature lower magnetic force stirring and dissolving, then solution semipermeable membrane distilled water is dialysed and remove acetic acid composition in 48 hours, make collagen solution;
(4) prepare synthetic Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 polypeptide, concrete grammar is: use automatic Peptide synthesizer according to following aminoacid sequence Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 synthetic Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 polypeptide;
(5) Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 covalent cross-linking modified collagen substrate is prepared, concrete grammar is: with 1-ethyl-(3, 3 '-dimethylaminopropyl) phosphinylidyne diimine and N-hydroxy-succinamide be covalent crosslinking agent, by described Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 polypeptide, 1-ethyl-(3, 3 '-dimethylaminopropyl) phosphinylidyne diimine and N-hydroxy-succinamide mix according to mol ratio 1: 1.2: 0.6, then be that 1%W/V adds in described collagen solution by mixture according to mass body volume concentrations, first magnetic agitation reacts 24 hours, then semipermeable membrane distilled water is dialysed and within 72 hours, is made Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 covalent cross-linking modified collagen substrate,
(6) citric acid soln of the agent of described self-curable calcium phosphate magnesium powder and 0.5mol/L is adjusted to mix according to mass volume ratio example and is mixed and made into self-curable calcium phosphate magnesium paste for 3.0g: 1ml, then with volume ratio 60%: 40% described self-curable calcium phosphate magnesium paste and described Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 covalent cross-linking modified collagen substrate adjusted to mix and be mixed and made into biological activity paste;
(7) two-part biological activity paste cure under pressure mould is prepared, epimere is used for holding and fixing described bizet (1), hypomere is for holding described root (2) and described biological activity paste, the surface of described root (2) is made to have the biological activity paste bag quilt of 3mm thickness, then by the split of described two-part biological activity paste pressurization fixed line mould together and be forced into 200-300kPa, be placed in 100% humidity environment and within 24 hours, make described calcium phosphate magnesium paste curing, freezing 12 hours in-80 DEG C again, vacuum lyophilization 48 hours, the collagen component in the crystallization of calcium phosphate magnesium is made to be solidified into dimensional fibre network-like structure, make the artificial tooth root with bionical natural teeth root of the tooth cementum structure,
(8) prepare artificial corona (8), make its form and agomphosis plesiomorphism, be fixed on described bizet (1) by screw (7), namely can be made into immediate implantation teeth.
2. have the manufacture method of the bioactive immediate implantation teeth of periodontal as claimed in claim 1, wherein, the connecting portion of described bizet (1) and described root (2) has the step (3) of 0.8-1mm.
3. there is the manufacture method of the bioactive immediate implantation teeth of periodontal as claimed in claim 2, wherein, finally form the thick bioactive layer (6) of 3mm at the outer surface of described root (2).
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| CN107007884A (en) * | 2017-03-09 | 2017-08-04 | 温州医科大学附属口腔医院 | Carry the purposes of the pure titanium implant material of cerium oxide nano particle functional film layer |
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