Summary of the invention
The technical problem solved: for the deficiency of the method for different heavy metal pollution acid grounds, as phytoremediation because the usual root system of plant that phytoremediation is used is flourishing not, can only the soil remediation on top layer be carried out and repairing efficiency is long, and can only carry out assembling in plant to certain heavy metal species; And chemical restoration is owing to easily causing biological property destroy and cause secondary pollution to soil.In order to improve deficiency, the invention provides a kind of restorative procedure of heavy metal pollution acid ground, the method is that one deep layer can repair various heavy and environmental friendliness, to the nondestructive fast method of geobiont character.
Technical scheme: in order to realize above-mentioned purpose of the present invention, the invention discloses a kind of restorative procedure of heavy metal pollution acid ground, and the step of described method comprises as follows:
(1) heavy metal pollution acid ground and calacareous soil are uniformly mixed, leave standstill 2h.
(2) add in mixed soil after a certain amount of urea phosphate, chitin, biochemical fulvic acid and alginic acid being mixed, stir.(3), after leaving standstill 3h, after adding bacillus-rice bran solution, a period of time is left standstill.
Preferably, the restorative procedure of described a kind of heavy metal pollution acid ground, the mass ratio of the heavy metal pollution acid ground described in step (1) and calacareous soil is 1:0.1 ~ 1:0.95.
Preferably, the restorative procedure of described a kind of heavy metal pollution acid ground, the urea phosphate described in step (2) is 25-35 part, chitin is 18-25 weight portion, biochemical fulvic acid is 10-20 weight portion, and alginic acid is 5-15 weight portion.
Preferably, the restorative procedure of described a kind of heavy metal pollution acid ground, in calacareous soil and step (2), the mass ratio of all additive total amounts is 1:0.01 ~ 1:0.1 in step (1).
Preferably, the restorative procedure of described a kind of heavy metal pollution acid ground, in step (3), bacillus is cultivated in advance in rice bran solution.
Further, preferably, the restorative procedure of described a kind of heavy metal pollution acid ground, bacillus is the seed liquor of 1 mL fixed concentration.
Further, preferably, the restorative procedure of described a kind of heavy metal pollution acid ground, rice bran initial concentration solution is 2-35 g/L.
Further, preferably, the restorative procedure of described a kind of heavy metal pollution acid ground, incubation time is 24h ~ 72h.
Preferably, the restorative procedure of described a kind of heavy metal pollution acid ground, after adding bacillus-rice bran solution in step (3), time of repose is 2-7 days.
Beneficial effect: the restorative procedure that the invention provides a kind of heavy metal pollution acid ground, the raw material that the method uses is all simple and easy to get, environmentally safe, secondary pollution can not be caused, that one deep layer can repair various heavy and environmental friendliness, to the nondestructive fast method of geobiont character, be applicable to commercially produce.
Detailed description of the invention
Below the embodiment of the present invention is described in detail, can be easier to make advantages and features of the invention be understood by this technical staff, thus more explicit defining is made to protection scope of the present invention.
Experimental technique described in following embodiment, if no special instructions, is conventional method; Described reagent and material, if no special instructions, all obtain by commercial channel.
Embodiment 1
Under room temperature, first, be that the heavy metal pollution acid ground (mark 1#, pH 4.0) of 1:0.1 leaves standstill 2h with calacareous soil mix and blend in mass ratio; Secondly, urea phosphate, chitin, biochemical fulvic acid and alginic acid mixture is added in mixed soil, the mass ratio of this amount of the mixture and calacareous soil is 1:0.01, wherein the content of added each component of mixing species is respectively: 25 parts of urea phosphates, 18 parts of chitins, 10 parts of biochemical fulvic acids and 5 parts of alginic acids, be uniformly mixed standing 3h; Finally, add the bacillus rice bran solution cultivating 24h in advance, wherein bacillus is the seed liquor of 1mL fixed concentration, and the concentration of rice bran solution is 2 g/L, mixes rear leaving standstill.Leave standstill after 2 days, heavy metal contaminated soil processes, and carries out concentration determination by atomic absorption spectrophotometer to the heavy metal of the leaching of contaminated soil.
Embodiment 2
Under room temperature, first, be that the heavy metal pollution acid ground (mark 1#, PH 4.0) of 1:0.95 leaves standstill 2h with calacareous soil mix and blend in mass ratio; Secondly, urea phosphate, chitin, biochemical fulvic acid and alginic acid mixture is added in mixed soil, the mass ratio of this amount of the mixture and calacareous soil is 1:0.1, wherein the content of added each component of mixing species is respectively: 35 parts of urea phosphates, 25 parts of chitins, 25 parts of biochemical fulvic acids and 15 parts of alginic acids, be uniformly mixed standing 3h; Finally, add the bacillus rice bran solution cultivating 72h in advance, wherein bacillus is the seed liquor of 1mL fixed concentration, and the concentration of rice bran solution is 35 g/L, mixes rear leaving standstill.Leave standstill after 7 days, heavy metal contaminated soil processes, and carries out concentration determination by atomic absorption spectrophotometer to the heavy metal of the leaching of contaminated soil.
Embodiment 3
Under room temperature, first, be that the heavy metal pollution acid ground (mark 1#, PH 4.0) of 1:0.5 leaves standstill 2h with calacareous soil mix and blend in mass ratio; Secondly, urea phosphate, chitin, biochemical fulvic acid and alginic acid mixture is added in mixed soil, the mass ratio of this amount of the mixture and calacareous soil is 1:0.5, wherein the content of added each component of mixing species is respectively: 25 parts of urea phosphates, 15 parts of chitins, 15 parts of biochemical fulvic acids and 10 parts of alginic acids, be uniformly mixed standing 3h; Finally, add the bacillus rice bran solution cultivating 48h in advance, wherein bacillus is the seed liquor of 1mL fixed concentration, and the concentration of rice bran solution is 20 g/L, mixes rear leaving standstill.Leave standstill after 5 days, heavy metal contaminated soil processes, and carries out concentration determination by atomic absorption spectrophotometer to the heavy metal of the leaching of contaminated soil.
Embodiment 4
Under room temperature, first, be that the heavy metal pollution acid ground (mark 2#, PH 4.0) of 1:0.8 leaves standstill 2h with calacareous soil mix and blend in mass ratio; Secondly, urea phosphate, chitin, biochemical fulvic acid and alginic acid mixture is added in mixed soil, the mass ratio of this amount of the mixture and calacareous soil is 1:0.7, wherein the content of added each component of mixing species is respectively: 32 parts of urea phosphates, 18 parts of chitins, 14 parts of biochemical fulvic acids and 15 parts of alginic acids, be uniformly mixed standing 3h; Finally, add the bacillus rice bran solution cultivating 48h in advance, wherein bacillus is the seed liquor of 1mL fixed concentration, and the concentration of rice bran solution is 24 g/L, mixes rear leaving standstill.Leave standstill after 5 days, heavy metal contaminated soil processes, and carries out concentration determination by atomic absorption spectrophotometer to the heavy metal of the leaching of contaminated soil.
Embodiment 5
Under room temperature, first, be that the heavy metal pollution acid ground (mark 3#, PH 4.0) of 1:0.5 leaves standstill 2h with calacareous soil mix and blend in mass ratio; Secondly, urea phosphate, chitin, biochemical fulvic acid and alginic acid mixture is added in mixed soil, the mass ratio of this amount of the mixture and calacareous soil is 1:0.5, wherein, the content of added each component of mixing species is respectively: 25 parts of urea phosphates, 15 parts of chitins, 15 parts of biochemical fulvic acids and 10 parts of alginic acids, be uniformly mixed standing 3h; Finally, add the bacillus rice bran solution cultivating 48h in advance, wherein bacillus is the seed liquor of 1mL fixed concentration, and the concentration of rice bran solution is 20 g/L, mixes rear leaving standstill.Leave standstill after 5 days, heavy metal contaminated soil processes, and carries out concentration determination by atomic absorption spectrophotometer to the heavy metal of the leaching of contaminated soil.
Comparison example 1
Under room temperature, first, be that the heavy metal pollution acid ground (mark 1#, PH 4.0) of 1:0.5 leaves standstill 2h with calacareous soil mix and blend in mass ratio; Secondly, urea phosphate, chitin, biochemical fulvic acid and alginic acid mixture is added in mixed soil, the mass ratio of this amount of the mixture and calacareous soil is 1:0.5, wherein, the content of added each component of mixing species is respectively: 25 parts of urea phosphates, 15 parts of chitins, 15 parts of biochemical fulvic acids and 10 parts of alginic acids, be uniformly mixed standing 3h.Leave standstill after 5 days, heavy metal contaminated soil processes, and carries out concentration determination by atomic absorption spectrophotometer to the heavy metal of the leaching of contaminated soil.
Comparison example 2
Under room temperature, first, be heavy metal pollution acid ground (the mark 1# of 1:0.25 in mass ratio, PH 4.0) with mixed soil in add urea phosphate, chitin, biochemical fulvic acid and alginic acid mixture, wherein the content of added each component of mixing species is respectively: 25 parts of urea phosphates, 15 parts of chitins, 15 parts of biochemical fulvic acids and 10 parts of alginic acids, be uniformly mixed standing 3h; Secondly, add the bacillus rice bran solution cultivating 48h in advance, wherein bacillus is the seed liquor of 1mL fixed concentration, and the concentration of rice bran solution is 20 g/L, mixes rear leaving standstill; Leave standstill after 5 days, heavy metal contaminated soil processes, and carries out concentration determination by atomic absorption spectrophotometer to the heavy metal of the leaching of contaminated soil.
Comparison example 3
Under room temperature, first, be that the heavy metal pollution acid ground (mark 1#, PH 4.0) of 1:0.5 leaves standstill 2h with calacareous soil mix and blend in mass ratio; Secondly, add the bacillus rice bran solution cultivating 48h in advance, wherein bacillus is the seed liquor of 1mL fixed concentration, and the concentration of rice bran solution is 20 g/L, mixes rear leaving standstill.Leave standstill after 5 days, heavy metal contaminated soil processes, and carries out concentration determination by atomic absorption spectrophotometer to the heavy metal of the leaching of contaminated soil.
The heavy metal initial content of table one three kinds of contaminated soils
Project |
Cadmium (Cd) |
Chromium (Cr) |
Plumbous (Pb) |
Soil (mark 1#) |
9.8 mg/kg |
342.4 mg/kg |
420.6 mg/kg |
Soil (mark 2#) |
15.5 mg/kg |
467.3 mg/kg |
572.3 mg/kg |
Soil (mark 3#) |
23.9 mg/kg |
530.8 mg/kg |
654.8 mg/kg |
The content that in table two example 1-5, after repair process, heavy metal is final
Project |
Soil is numbered |
Cadmium (Cd) |
Chromium (Cr) |
Plumbous (Pb) |
Example 1 |
Soil (mark 1#) |
0.3 mg/kg |
16.7 mg/kg |
13.2 mg/kg |
Example 2 |
Soil (mark 1#) |
0.2 mg/kg |
8.4 mg/kg |
6.9 mg/kg |
Example 3 |
Soil (mark 1#) |
0.1 mg/kg |
10.8 mg/kg |
9.3 mg/kg |
Example 4 |
Soil (mark 2#) |
0.4 mg/kg |
13.1 mg/kg |
11.5 mg/kg |
Example 5 |
Soil (mark 3#) |
0.6 mg/kg |
19.8 mg/kg |
14.7 mg/kg |
The content that after repair process in table three comparison example 1-3, heavy metal is final
Project |
Cadmium (Cd) |
Chromium (Cr) |
Plumbous (Pb) |
Comparison example 1 |
6.5 mg/kg |
176.7 mg/kg |
144.9 mg/kg |
Comparison example 2 |
4.8 mg/kg |
132.5 mg/kg |
121.6 mg/kg |
Comparison example 3 |
5.7 mg/kg |
184.3 mg/kg |
207.8 mg/kg |
From table one and table two result, the heavy metal pollution acid ground restorative procedure heavy metal cadmium, chromium and the lead that used are all effective, and the soil of heavy metal content cadmium (≤23.9 mg/kg), chromium (≤530.8 mg/kg) and lead (≤654.8 mg/kg) can obtain good repairing effect.From the comparison example of table three, in method used in the present invention: the lime soil in step (1), urea phosphate in step (2), chitin, biochemical fulvic acid and alginic acid mixture, and the bacillus rice bran solution in step (3), three is all indispensable, there is cooperative effect between three, collaborate completes the reparation to acid ground heavy metal.