CN104480036B - Microbial flocculant proteus vulgaris and its application in cane juice clarification - Google Patents
Microbial flocculant proteus vulgaris and its application in cane juice clarification Download PDFInfo
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Abstract
Application the invention discloses microbial flocculant proteus vulgaris and its in cane juice clarification.The producing strains of the microbial flocculant are proteus vulgaris (Proteus vulgaris) N 25, and deposit number is CCTCC NO:M2014549, is preserved in China typical culture collection center on November 5th, 2014.Using when, phosphoric acid is added in Cane Mixed Juice, to be heated to 50~70 DEG C after milk of lime regulation pH to 68, insulation；It is 1.5~2.0g/L to add sulfurous acid to stove drying amount, and 60~100 DEG C are heated to after 79 after regulation pH, adds microbial flocculant to stand 20~40min.Microbial flocculant consumption of the present invention is few, and flocculation efficiency is high, and microbial flocculant safety non-toxic, easily biological-degradable are applied to sugar industry, can produce the product of more health environment-friendly.
Description
Technical field
The present invention relates to a kind of microbial flocculant, more particularly to microbial flocculant proteus vulgaris (Proteus
Vulgaris) N-25 and its application in cane juice clarification.
Background technology
Flocculant is that a class can make solid suspended particle flocculation, the material of precipitation of not free settling in liquid.Sugaring process
In, a small amount of flocculant is added in by the sugarcane juice for adding ash, the sediment in juice can be made to flocculate into relatively thicker agglomerate, so that
Improve sinking speed.At present, sugar refinery commonly uses polyacrylamide as flocculant, monomer acrylamide have mutagenesis,
Teratogenesis and carcinogenesis, remaining or enter into syrup influences sugar products safety in the middle of commerieal sugar.Therefore, highly effective and safe is sought
Flocculant oneself turn into China's sugar industry problem demanding prompt solution.
Microbial flocculant is the metabolite for having flocculation activity that a class is produced by microorganism, mainly contain glycoprotein,
Mucopolysaccharide, cellulose and nucleic acid etc., be obtained from biotechnology passes through microbial fermentation, separation and Extraction it is a kind of it is new,
Efficient flocculant.There are many unique properties and advantage compared with traditional inorganic and organic synthesis high polymer coagulant：
1. it is easy to separation of solid and liquid, forms sediment few；2. easily it is degraded by microorganisms, it is nontoxic, it is safe；3. non-secondary pollution.
Not the features such as some microbial flocculants also have not to be influenceed by pH conditions, heat endurance is strong, consumption is small.In addition flocculant is produced
Microorganism is most from the soil very close with human relation, and resource is extremely enriched, and preparation method is fairly simple.By
In microbial flocculant both some characteristics with microorganism, and the characteristic with natural macromolecule flocculating agent, can overcome inorganic
The deficiency of flocculant and organic polymer coargulator, is the study hotspot and developing direction of current flocculant.
Microbial flocculant reaches good at aspects such as treatment heavy metal wastewater thereby, waste water fermentation, low-temperature wastewater, dyeing waste waters
Good application effect.But it is less in the research of sugar industry application aspect, and rarely have at proteus vulgaris producing microbial flocculant
Manage the report of sugarcane juice.Microbial flocculant is applied to sugar manufacturing industry, it is raw to meet requirement of the people to food quality and safety
The product of output more health environment-friendly is significant.
The content of the invention
Refractory organicses, " three cause " effect it is an object of the invention to be directed to the polyacrylamide that current cane sugar factory is used
The problems such as, screening is adapted to the bacterium for producing flocculant of microbe proteus vulgaris (Proteus of sugar industry cane juice clarification
Vulgaris) N-25, there is provided a kind of safe and nontoxic, free of contamination microbial flocculant substitutes the organic polymer for having used and wads a quilt with cotton
Solidifying agent polyacrylamide.
The object of the invention is achieved through the following technical solutions：
Microbial flocculant proteus vulgaris, the producing strains of the microbial flocculant are proteus vulgaris (Proteus
Vulgaris) N-25, deposit number is CCTCC NO:M2014549.
The 16S rDNA sequences of proteus vulgaris (Proteus vulgaris) N-25 are SEQ ID NO:1.
Application of the microbial flocculant proteus vulgaris in cane juice clarification technique.
Using when, phosphoric acid is added in Cane Mixed Juice, with P2O5Meter, controls phosphoric acid concentration for 300~400ppm, regulation
50~70 DEG C, insulation are heated to after pH to 6-7；It is 1.5~2.0g/L to add sulfurous acid to stove drying amount, after regulation pH after 6.8-9
Be heated to 60~100 DEG C, add microbial flocculant to stand, microbial flocculant be the volume of pending sugar-cane juice 1%~
5%.Such as：Cane Mixed Juice 100ml is taken, adds the μ l of phosphatase 24 0~60 that mass fraction is 80~90%, plus milk of lime to be adjusted to pH
It is 6~7.0, sulfurous acid stove drying is added after being heated to 50~70 DEG C, stove drying amount is 1.5~2.0g/L, then adjusts pH extremely with milk of lime
6.8~9.0, reheating adds 1~5ml of microbial flocculant, measurement sedimentation 50ml required times to 60~100 DEG C.Stand
Mud bottom volume is measured after 20~40min, 0.45 μm of filtering with microporous membrane of supernatant is taken, pol, the refractive power of filtrate are surveyed respectively
Absorbance at degree and 560nm, calculates the simple purity and colour of sugarcane juice.
Preferably, the regulation pH to 6-7 is adjusted by adding milk of lime.
Preferably, the time of the insulation is 2~5min.
Preferably, the time of the standing is 20~40min.
Microbial flocculant proteus vulgaris of the present invention obtains as follows：
(1) active sludge microorganism is isolated and purified
Take Guangzhou sludge sewage Sample Dilution to 10-1~10-7Different volumes concentration gradient after, take
10-4、10-5、10-6、10-7Sludge microbe is inoculated in agar medium with spread plate method.In 30~35 DEG C of constant temperature after inoculation
Cultivated 1~3 day in incubator, the isolated multiple-microorganism bacterium colony on plating medium.Plate streak picking colony in
Culture dish is enlarged culture.Repeat the above steps, the microorganism of different colonial morphologies, inoculation are obtained after being isolated and purified through 5~6 times
Onto slant medium, in 4 DEG C of refrigerator storages.
(2) screening of bacterium for producing flocculant
After strain liquid Amplification Culture after isolating and purifying, screened by index of 2~5g/L aqueous suspension ofkaolins, had
Body method is：In the 100ml liquid of the aqueous suspension ofkaolin equipped with 2~5g/L, it is 1~2% to add 3~5ml mass fractions
CaCl2Solution adds the microbial flocculant sample of 1-3ml as flocculation aid, adjusts pH value to 7.4~7.6, is stood after stirring
5~10min, measures.Using bacterial strain of the flocculating rate more than 80% as aimed strain, the high activity flocculant that secondary screening must be stablized
Producing strains.
3) by high-activity microorganism bacterium for producing flocculant access fermentation medium, cultivation temperature is 30~40 DEG C, shaking table
Rotating speed is 140~200r/min, and fermented and cultured 3~4 days, zymotic fluid is centrifuged 20min, takes supernatant in 6000~8000r/min
As microbial flocculant.Fermentation medium is constituted：15~20g/L of glucose, 0.4~0.6g/L of urea, yeast extract 0.4~
0.7g/L, 0.1~0.2g/L of ammonium sulfate, 3~5g/L of dipotassium hydrogen phosphate, 1~3g/L of potassium dihydrogen phosphate, sodium chloride 0.05~
0.2g/L, 0.1~0.25g/L of magnesium sulfate, initial pH7~8.
Relative to prior art, the present invention has the advantages that：
(1) present invention directly screens bacterium for producing flocculant of microbe from sludge sewage, produces flocculant bacterial strain
Wide material sources, cultivate 1~3 day after the inoculation of spread plate method in 30~35 DEG C of constant incubators, repeat purifying 5~6 times
Separate, purify a large amount of different types of bacterial strains, strain growth metabolism is fast, and the cycle is short for isolating and purifying, it is easy to operate is suitable for rule
Modelling is produced.
(2) with bacterial strain to the flocculating rate of aqueous suspension ofkaolin as index, from above-mentioned steps bacterial strain after purification, screening
High-activity microorganism bacterium for producing flocculant is to the flocculating rate of aqueous suspension ofkaolin more than 90%.Aqueous suspension ofkaolin is clear with sugarcane juice
Settle accounts fruit and keep high consistency, it is ensured that the bacterial strain of screening is being applied to cane juice clarification effect.
(3) flcos producing bacteria that screening is obtained is 30~40 DEG C, oscillation and fermentation cultivation under the rotating speed of 140~200r/min in temperature
3~4 days, microbial flocculant cultivation temperature was normal temperature condition, and rotating speed and energy consumption are relatively low and be easily achieved, relative chemical flocculant
Production cost substantially reduce.
(4) after microbial flocculant treatment sugarcane juice, sugarcane juice letter purity is more than 84.0%, and colour rate is more than 74.9%,
Flocculating property is significantly improved, and the clarifying effect of microbial flocculant is better than chemical floc polyacrylamide, and sinking speed is fast,
Mud bottom small volume after flocculation, is easy to treatment.Compared with after polyacrylamide treatment, the sedimentation time shortens more than 6min, finally
Mud bottom volume compression more than 8ml, sugarcane juice letter purity improves more than 1.7%, and percent of decolourization improves more than 12.7%.
(5) microbial flocculant that proteus vulgaris produces is applied to cane juice clarification technique by the present invention first, and clear
Clear effect is significant, can provide new thinking for cane juice clarification technique.
(6) the product flocculated bacteria of screening can effectively improve cane juice clarification effect, and with easily biological-degradable, high effect nontoxic etc.
Outstanding advantages, can avoid the secondary pollution problems of chemical floc, be the new and effective flocculation having broad application prospects
Agent.
Brief description of the drawings
Fig. 1 is pure with microbial flocculant treatment sugarcane juice sedimentation time, mud bottom volume, letter for the present invention implements polyacrylamide
Degree, percent of decolourization comparison diagram.Abscissa is followed successively by sedimentation time, mud bottom volume, simple purity, percent of decolourization in figure；Ordinate is that double Y sit
Mark, respectively sedimentation time/min, mud bottom volume/ml and letter purity/%, percent of decolourization/%.
Specific embodiment
To more fully understand the present invention, with reference to embodiment, the present invention will be further described, but implementation of the invention
Mode not limited to this.
The formula of the culture medium used in following examples is as follows：
Screening and culturing medium：Beef extract 5g/L, peptone 10g/L, sodium chloride 5g/L, agar 20g/L, pH7.5,121 DEG C high
Pressure steam sterilizing 30min.
Fluid nutrient medium：Beef extract 5g/L, peptone 10g/L, sodium chloride 5g/L, pH7.5,121 DEG C high pressure steam sterilization
30min。
Fermentation medium：Glucose 20g/L, urea 0.5g/L, yeast extract 0.5g/L, ammonium sulfate 0.2g/L, phosphoric acid hydrogen two
Potassium 5g/L, potassium dihydrogen phosphate 2g/L, sodium chloride 0.1g/L, magnesium sulfate 0.2g/L, initial pH7.0,115 DEG C of high pressure steam sterilizations
20min。
Embodiment 1
Guangzhou sludge sewage sample 1mL is taken, 10 are diluted to-1~10-7Different volumes concentration gradient
Afterwards, 10 are taken-4、10-5、10-6、10-7Sludge microbe is inoculated in agar medium with spread plate method.In 30 DEG C of perseverances after inoculation
Cultivated 2 days in warm incubator, the isolated multiple-microorganism bacterium colony on plating medium.Plate streak picking colony is in training
Foster ware is enlarged culture.Repeat the above steps, the microorganism of different colonial morphologies is obtained after being isolated and purified through 5 times, be inoculated into tiltedly
On the culture medium of face, in 4 DEG C of refrigerator storages.Again respectively will after purification bacterial strain access 50ml screening and culturing mediums in, put 35 DEG C, 150r/
Min Shaking cultures 3 days, the Preliminary Determination of flocculation activity is carried out using following methods to gained nutrient solution.
In the 100ml liquid of the aqueous suspension ofkaolin equipped with 5g/L, the CaCl that 5ml mass fractions are 1% is added2Solution is made
It is flocculation aid, adds the microbial flocculant sample of 2ml, adjust pH value to 7.5,5min is stood after stirring, uses spectrophotometer
It is the absorbance at 550nm that supernatant is determined in wavelength, while replacing testing sample as control using distilled water, micrometer is treated in calculating
The flocculating rate of biological flocculant sample：Flocculating rate (%)=(B of A mono-)/A × 100%, A is the suction for compareing supernatant at 550nm
Luminosity, B is absorbance of the sample supernatant at 550nm.Using bacterial strain of the flocculating rate more than 80% as aimed strain, secondary screening is obtained
The high activity bacterium for producing flocculant of stabilization.The microbial flocculant of proteus vulgaris N-25 productions is wadded a quilt with cotton to aqueous suspension ofkaolin
Solidifying effect is significant, its flocculating rate is up to 92.6%, and the kaolinite scholar's particle aggregation degree after treatment is improved, suspension haze reduction.
Obtained strains bacterium colony surface is smooth translucent, and uniform thin layer, colonial morphology are extended on nutrient agar surface
It is direct rod shape.Its Physiology and biochemistry qualification result is as shown in table 1, DNA sequencing result such as SEQ ID NO:Shown in 1, by BLAST pairs
Than being up to 99.9% with proteus vulgaris (Proteus vulgaris) homology.Through strain morphology and Physiology and biochemistry and
16SrDNA gene orders are accredited as proteus vulgaris (Proteus vulgaris), are named as proteus vulgaris
(Proteus vulgaris)N-25。
Microbial flocculant proteus vulgaris (Proteus vulgaris) N-25 obtained by the present embodiment is in 2014
On November 5, in is preserved in China typical culture collection center, and numbering is CCTCC NO:M2014549.Preservation address is：Hubei
Wuhan City of province Hongshan District Bayi Road Wuhan University collection, postcode：430072.
The identification of the high activity bacterium for producing flocculant Physiology and biochemistry of table 1 (+：It is positive；-, it is negative)
Embodiment 2
Embodiment 1 is screened microbial flocculant proteus vulgaris (Proteus vulgaris) N-25 inoculations for obtaining
Cultivated 24 hours for 30 DEG C in the test tube equipped with seed culture fluid.It is then seeded into fermented and cultured in fermentation culture, fermentation temperature
It is 35 DEG C to spend, and shaking speed is 150rpm, and fermentation time is 4 days.Fermentation culture is centrifuged 30min under 6000r/min, is taken
Supernatant is microbial flocculant.
Embodiment 3
Cane Mixed Juice 100ml is taken, the μ L of phosphoric acid 50 that mass fraction is 85% are added, it is 6.8 to be adjusted to pH with milk of lime, plus
Thermal agitation is to being incubated 2min after 60 DEG C.The sulfurous acid stove drying that mass fraction is 6% is added, stove drying amount is 1.8g/L, then uses lime
Breast neutralizes pH to 7.1, and reheating rapidly joins microbial flocculant 2ml or 0.1% polyacrylamide 0.2ml to 100 DEG C,
Stood after stirring.The time required to record sedimentation 50ml.Mud bottom volume is measured after 30min, supernatant is taken with 0.45 μm of micropore
Membrane filtration, the absorbance surveyed respectively at pol, diopter and the 560nm of filtrate calculates the simple purity and colour of sugarcane juice.Letter
The measure and calculating of purity and colour are pressed《Cane sugar manufacture chemistry management analysis method》Method is measured and calculates, as a result as schemed
Shown in 1.From figure 1 it appears that microbial flocculant treatment sugarcane juice, compared with after polyacrylamide treatment, sedimentation time contracting
Short more than 6min, final mud bottom volume compression more than 8ml, sugarcane juice letter purity improves more than 1.7%, and percent of decolourization improves
More than 12.7%.
Embodiment 4
Cane Mixed Juice 100ml is taken, the μ L of phosphoric acid 50 that mass fraction is 85% are added, it is 6 to be adjusted to pH with milk of lime, heating
Stirring is to being incubated 2min after 50 DEG C.The sulfurous acid stove drying that mass fraction is 6% is added, stove drying amount is 1.8g/L, then uses milk of lime
PH to 7.1 is neutralized, reheating rapidly joins microbial flocculant 1ml to seething with excitement, and is stood after stirring.Record sedimentation
The time required to 50ml.Mud bottom volume is measured after 30min, 0.45 μm of filtering with microporous membrane of supernatant is taken, turning for filtrate is surveyed respectively
Absorbance at luminosity, diopter and 560nm, calculates the simple purity and colour of sugarcane juice.The measure and calculating of simple purity and colour
Press《Cane sugar manufacture chemistry management analysis method》Method is measured and calculates.The sedimentation time is obtained for 15.9min, final mud bottom
Highly it is 38.3ml, simple purity is 74.1%, and percent of decolourization is 67.5%.
Embodiment 5
Cane Mixed Juice 100ml is taken, the μ L of phosphoric acid 50 that mass fraction is 85% are added, it is 7 to be adjusted to pH with milk of lime, heating
Stirring is to being incubated 2min after 60 DEG C.The sulfurous acid stove drying that mass fraction is 6% is added, stove drying amount is 1.8g/L, then uses milk of lime
PH to 7.1 is neutralized, reheating rapidly joins microbial flocculant 3ml to seething with excitement, and is stood after stirring.Record sedimentation
The time required to 50ml.Mud bottom volume is measured after 30min, 0.45 μm of filtering with microporous membrane of supernatant is taken, turning for filtrate is surveyed respectively
Absorbance at luminosity, diopter and 560nm, calculates the simple purity and colour of sugarcane juice.The measure and calculating of simple purity and colour
Press《Cane sugar manufacture chemistry management analysis method》Method is measured and calculates.The sedimentation time is obtained for 14.5min, final mud bottom
Highly it is 36.7ml, simple purity is 74.5%, and percent of decolourization is 72.5%.
Embodiment 6
Cane Mixed Juice 100ml is taken, the μ L of phosphoric acid 50 that mass fraction is 85% are added, it is 6.8 to be adjusted to pH with milk of lime, plus
Thermal agitation is to being incubated 2min after 60 DEG C.The sulfurous acid stove drying that mass fraction is 6% is added, stove drying amount is 1.8g/L, then uses lime
Breast neutralizes pH to 7.4, and reheating rapidly joins microbial flocculant 2ml to seething with excitement, and is stood after stirring.Record sedimentation
The time required to 50ml.Mud bottom volume is measured after 30min, 0.45 μm of filtering with microporous membrane of supernatant is taken, turning for filtrate is surveyed respectively
Absorbance at luminosity, diopter and 560nm, calculates the simple purity and colour of sugarcane juice.The measure and calculating of simple purity and colour
Press《Cane sugar manufacture chemistry management analysis method》Method is measured and calculates.The sedimentation time is obtained for 16.9min, final mud bottom
Highly it is 43.3ml, simple purity is 73.7%, and percent of decolourization is 64.3%.
Embodiment 7
Cane Mixed Juice 100ml is taken, the μ L of phosphoric acid 50 that mass fraction is 85% are added, it is 6.8 to be adjusted to pH with milk of lime, plus
Thermal agitation is to being incubated 2min after 60 DEG C.The sulfurous acid stove drying that mass fraction is 6% is added, stove drying amount is 1.8g/L, then uses lime
Breast neutralizes pH to 7.7, and reheating rapidly joins microbial flocculant 2ml to seething with excitement, and is stood after stirring.Record sedimentation
The time required to 50ml.Mud bottom volume is measured after 30min, 0.45 μm of filtering with microporous membrane of supernatant is taken, turning for filtrate is surveyed respectively
Absorbance at luminosity, diopter and 560nm, calculates the simple purity and colour of sugarcane juice.The measure and calculating of simple purity and colour
Press《Cane sugar manufacture chemistry management analysis method》Method is measured and calculates.The sedimentation time is obtained for 14..5min, final mud
Bottom is highly 40.0ml, and simple purity is 74.3%, and percent of decolourization is 67.5%.
Embodiment 8
Cane Mixed Juice 100ml is taken, the μ L of phosphoric acid 50 that mass fraction is 85% are added, it is 6.8 to be adjusted to pH with milk of lime, plus
Thermal agitation is to being incubated 2min after 60 DEG C.The sulfurous acid stove drying that mass fraction is 6% is added, stove drying amount is 1.8g/L, then uses lime
Breast neutralizes pH to 7.1, and 80 DEG C of reheating rapidly joins microbial flocculant 2ml, is stood after stirring.Record sedimentation
The time required to 50ml.Mud bottom volume is measured after 30min, 0.45 μm of filtering with microporous membrane of supernatant is taken, turning for filtrate is surveyed respectively
Absorbance at luminosity, diopter and 560nm, calculates the simple purity and colour of sugarcane juice.The measure and calculating of simple purity and colour
Press《Cane sugar manufacture chemistry management analysis method》Method is measured and calculates.The sedimentation time is obtained for 14.8min, final mud bottom
Highly it is 40.6ml, simple purity is 72.6%, and percent of decolourization is 53.5%.
Embodiment 9
Cane Mixed Juice 100ml is taken, the μ L of phosphoric acid 50 that mass fraction is 85% are added, it is 6.8 to be adjusted to pH with milk of lime, plus
Thermal agitation is to being incubated 2min after 60 DEG C.The sulfurous acid stove drying that mass fraction is 6% is added, stove drying amount is 1.8g/L, then uses lime
Breast neutralizes pH to 7.1, and reheating rapidly joins microbial flocculant 2ml to 90 DEG C, is stood after stirring.Record sedimentation
The time required to 50ml.Mud bottom volume is measured after 30min, 0.45 μm of filtering with microporous membrane of supernatant is taken, turning for filtrate is surveyed respectively
Absorbance at luminosity, diopter and 560nm, calculates the simple purity and colour of sugarcane juice.The measure and calculating of simple purity and colour
Press《Cane sugar manufacture chemistry management analysis method》Method is measured and calculates.The sedimentation time is obtained for 13.1min, final mud bottom
Highly it is 39.2ml, simple purity is 74.7%, and percent of decolourization is 76.8%.
The present invention processes sugarcane juice using microbial flocculant, and sugarcane juice letter purity is more than 84.0%, and colour rate is 74.9%
More than, flocculating property is significantly improved, and the clarifying effect of microbial flocculant is better than chemical floc polyacrylamide, sedimentation speed
Degree is fast, mud bottom small volume after flocculation, is easy to treatment.Compared with after polyacrylamide treatment, the sedimentation time shortens more than 6min,
Final mud bottom volume compression more than 8ml, sugarcane juice letter purity improves more than 1.7%, and percent of decolourization improves more than 12.7%, comprehensive
Effect is closed to significantly improve.
The microbial flocculant that proteus vulgaris produces is applied to cane juice clarification technique, and clarification effect by the present invention first
Fruit significantly, can provide new thinking for cane juice clarification technique.
Importantly, the product flocculated bacteria of present invention screening can effectively improve cane juice clarification effect, and the flocculation for producing
Agent is made up of glycoprotein, mucopolysaccharide, cellulose and nucleic acid etc., with outstanding advantages such as easily biological-degradable, high effect nontoxics, can avoid
The secondary pollution problems of chemical floc, are the new and effective flocculants having broad application prospects, and can be produced more strong
The product of Kang Huanbao.
Claims (7)
1. microbial flocculant proteus vulgaris, it is characterised in that：The producing strains of the microbial flocculant are common variation
Bacillus (Proteus vulgaris) N-25, deposit number is CCTCC NO:M2014549.
2. microbial flocculant proteus vulgaris according to claim 1, it is characterised in that the proteus vulgaris
The 16S rDNA sequences of (Proteus vulgaris) N-25 are SEQ ID NO:1.
3. application of the microbial flocculant proteus vulgaris in cane juice clarification technique described in claim 1.
4. application of the microbial flocculant proteus vulgaris according to claim 3 in cane juice clarification technique, it is special
Levy and be, using when, phosphoric acid is added in Cane Mixed Juice, with P2O5Meter, controls phosphoric acid concentration for 300~400ppm, adjusts pH
50~70 DEG C, insulation are heated to after to 6-7；It is 1.5~2.0g/L to add sulfurous acid to stove drying amount, is added after regulation pH to 6.8-9
Heat to 60~100 DEG C, add microbial flocculant stand, microbial flocculant be the volume of pending sugar-cane juice 1%~
5%.
5. application of the microbial flocculant proteus vulgaris according to claim 4 in cane juice clarification technique, it is special
Levy and be, the regulation pH to 6-7 is adjusted by adding milk of lime.
6. application of the microbial flocculant proteus vulgaris according to claim 4 in cane juice clarification technique, it is special
Levy and be, the time of the insulation is 2~5min.
7. application of the microbial flocculant proteus vulgaris according to claim 4 in cane juice clarification technique, it is special
Levy and be, the time of the standing is 20~40min.
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| CN101070203A (en) * | 2007-06-18 | 2007-11-14 | 同济大学 | Method for preparing biological flocculant using strange deformation bacilli |
| CN101503709A (en) * | 2009-03-13 | 2009-08-12 | 厦门大学 | Method for preparing bioflocculation by Bacillus licheniformis |
| CN101899536A (en) * | 2010-09-01 | 2010-12-01 | 厦门大学 | Application of bioflocculant synthesized by bacillus licheniformis in refining sugar |
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| CN101070203A (en) * | 2007-06-18 | 2007-11-14 | 同济大学 | Method for preparing biological flocculant using strange deformation bacilli |
| CN101503709A (en) * | 2009-03-13 | 2009-08-12 | 厦门大学 | Method for preparing bioflocculation by Bacillus licheniformis |
| CN101899536A (en) * | 2010-09-01 | 2010-12-01 | 厦门大学 | Application of bioflocculant synthesized by bacillus licheniformis in refining sugar |
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