CN104450944A - Technical method for quickly detecting purity of north high-quality hybrid japonica rice Longyou 619 - Google Patents
Technical method for quickly detecting purity of north high-quality hybrid japonica rice Longyou 619 Download PDFInfo
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Abstract
The invention relates to a technical method for quickly detecting the purity of north high-quality hybrid japonica rice Longyou 619. The invention solves the problems that traditional field planting detection is wasting in labor and time, and the detection result is easy to be influenced by environment, and can early foresee the purity of hybrid, thus being convenient to arrange a next work plan. The method includes the following steps of: 1, extracting DNA of a material to be detected; 2, aiming at the characteristics of parents of Longyou 619, using the molecular marker InDel-Rfla of a restore gene Rfla and the InDel funtioanl marker GRFM04 of a fragrance gene fgr in the patent to perform PCR amplification detection on the extracted DNA; 3, comparing the amplified DNA segment for analyzing. The marker set is used for detecting the purity of the rice hybrid and authenticating true or false of the same, has the basic characteristics of environmental stability, identifiability in variety variations and reliability in experimental results, also has the advantages of being quick, simple, convenient and low in cost, and is applicable to the aspects of quick analysis of purity of large sample hybrid Longyou 619, detection and authentication of fake varieties, molecular marker-assisted breeding and the like.
Description
Technical field
The present invention relates to a kind of technological method utilizing molecule marker rapid detection Japonica Hybrid 619 purity.The method is mainly according to breediness, rely on molecular detection technology, utilize molecule marker to identify hybrid rice seeds gene fragment, owing to being the difference directly reflecting DNA level, so accuracy rate can reach 99.99%, and qualification time shortens to 3-4 hour.
Background technology
Paddy rice is the first food crop of China, accounts for 1/3 of View of World Rice ultimate production.Current rice breeding method is still based on cross-breeding and heterosis utilization, and other method is auxiliary.By cross-breeding, paddy rice achieves quantum jump in output.Hybridisation rice sown area is large, and seed sale profit is high, occupies very critical role in China's grain-production.Hybrid rice seeds purity quality directly affects power and the output height of heterosis, hybrid vigor.Therefore, from seed produces, until receive, transport, store, the process such as to sell in all to pay close attention to and strictly prevent biology from mixing and mechanical admixture.In addition, the event of cheating the farmers caused of artificially faking once brought massive losses to agriculture production.
The purity detecting of seed is an important process on cross-fertilize seed produces, and is directly connected to the development of agricultural and the vital interests of peasant, is therefore subject to the great attention of country.In order to ensure production kind of a safety, conventional seed purity identification method is that Hainan adds generation plantation, carries out Morphological Identification.Namely grow test by field planting and differentiate seed purity, but this detection method is time-consuming takes a lot of work, and be unfavorable for next step job placement, and detected result is subject to the season of growth and environmental limit, be unfavorable for the commercialization running of Seed Identification and judicially put to the proof.Therefore, according to rice varieties feature, the purity that binding molecule detects cenospecies is a well selection.
Current protein fingerprint authenticate technology has that resolving power is high, polymorphism is high, and has repeatability, the individual specificity of height and environmental stability, therefore can differentiate the fine difference between biont, be used to the Purity etc. of seed.The U.S., Canada, France this Technology application in the seed true and false and purity detecting, but utilize the method to detect seed spring sister-in-law in China also to have difficulties, reason is that the method is complicated, technical difficulty large, cost intensive.Over nearly 20 years, along with deepening continuously of genome plan research, DNA molecular marker obtains and develops rapidly and be widely used in the every field of molecular biology research.The molecule marker grown up reaches kind more than 60, and as RFLP, RFAP, SSR, SNP, InDel etc., these marks provide solid technical foundation for various cultivar identification undoubtedly.
Many molecule markers no doubt can identify purity and the verity of cross combination, but add Financial cost and the time of qualification undoubtedly.Therefore, in cenospecies production process, according to the special gene type that Parent contains, reduce the use of molecule marker, reach the desired result of Purity.This research, in the grand male parent of excellent 619 of northern Quality Hybrid Japonica Rice and the maternal basis detected containing Restoring gene Rf 1 a and scent gene fhr, carries out the qualification of purity with the molecular function mark of these two genes to cenospecies.
Summary of the invention
The object of the present invention is to provide the grand technological method of excellent 619 of a kind of rapid detection north Quality Hybrid Japonica Rice, this cover method is used for the grand Purity of excellent 619, not only there is the fundamental characteristics such as the stability of environment, the reliability of experimental result, application also has qualification that is quick, easy, low cost, is applicable to the seed purity real-time analysis of large sample, the Testing and appraisal of fake and forged kind and molecular mark.The method is simple laboratory operation by originally a large amount of field trapping test work changes, the grand purity of excellent 619 of more accurate rapid detection, saves manpower, increases work efficiency and accuracy.Protection breed breeding and the legitimate rights and interests of lawful operation person and the scientific and technical innovation of breeding research will be promoted effectively.
For realizing above object, technical scheme steps of the present invention is as follows:
1. a technological method for grand excellent 619 purity of rapid detection north Quality Hybrid Japonica Rice, is characterized in that:
Northern Quality Hybrid Japonica Rice grand excellent 619 for results carries out presoaking and germinating, DNA extraction, pcr amplification, electrophoresis detection and analysis, finally adds up purity of hybrid.
2. method according to claim 1, is characterized in that: have randomness when the northern Quality Hybrid Japonica Rice of results grand excellent 619 is soaked seed in step 1, and seed moisture content controls about 14%, meets field planting rule.
3. method according to claim 1, is characterized in that: in step 1 during presoaking and germinating, and 50 ~ 100 seeds selected by each culture dish, add clear water 15ml, 25 ~ 28 DEG C about illumination cultivation 10-15 days, treat that seedling grows to the 3-4 leaf phase and starts sampling.Have randomness during sampling, do not distinguish seedling size, power, the root of each whole plant, stem, leaf put into a 2ml centrifuge tube, and sampled population can experimentally conditional decision, more than at least 100 strains, in order to DNA extraction.
4. method according to claim 1, it is characterized in that: DNA extraction in step 1, put into the plain ball that diameter 2.75mm and 4.75mm varies in size in the 2ml centrifuge tube of application of sample, add 800 μ l SDS extracting solutions, high-throughput tissue grinder grinds, and frequency is 55HZ, the time is 180S.Grind rear 65 DEG C of water-baths about 30 minutes.Add 800 μ l phenol in centrifuge tube after water rain: chloroform: primary isoamyl alcohol 25: 24: 1, volume ratio, fully mixes, the centrifugal 10min of desk centrifuge 10000rpm, gets supernatant 500 μ l and is transferred to another 1.5ml centrifuge tube.Then carry out second time extracting, add 500 μ l phenol: chloroform: primary isoamyl alcohol 25: 24: 1, volume ratio, fully mixes, the centrifugal 10min of desk centrifuge 10000rpm, gets supernatant 400 μ l and is transferred to another 1.5ml centrifuge tube.Add 320 μ l Virahols, mixing, 4 DEG C or place 20min on ice, the centrifugal 10min of 10000rpm, outwells supernatant liquor, centrifuge tube is inverted about 10-15min and is filtered dry alcohol, and then DNA is dissolved in 100 μ l distilled waters ,-20 DEG C save backup.DNA solution dilutes 15 times, for pcr amplification.
5. method according to claim 1, is characterized in that: in step 1 during pcr amplification, selects molecule marker according to the grand breediness of excellent 619.Through gene test, grand excellent 619 female parents are containing scent gene fgr, male parent contains Restoring gene Rf 1 a, so select the related molecular marker GRFM04 (F:3 '-GTTAGGTTGCATTTACTGGGAG-5 ' of scent gene, R:3 '-GAATGATGCTCAAAGTGTCT-5 ') and the related molecular marker InDel-Rf1a (F:3 '-CTGATGATCGAGGAGGAGGTA-5 ', R:3 '-TAACGCGTCTTCCATCCTACT-5 ') of Restore gene carry out pcr amplification.
(1) amplification reaction system is as follows:
(2) amplification reaction condition
The related molecular marker GRFM04 amplification reaction condition of scent gene is as follows:
94 DEG C of denaturation 5min; 94 DEG C of 1min, 55 DEG C of 1min, 72 DEG C of 2min, totally 35 circulations; Finally carry out 72 DEG C and extend 5min.4 DEG C of preservations are stand-by.
The related molecular marker InDel-Rf1a amplification reaction condition of Restore gene is as follows:
94 DEG C of denaturation 5min; 94 DEG C of 30s, 55 DEG C of 30s, 72 DEG C of 1min, totally 35 circulations; Finally carry out 72 DEG C and extend 7min.4 DEG C of preservations are stand-by.
(3) electrophoresis detection: the product polyacrylamide gel electrophoresis after the related molecular marker GRFM04 amplification of scent gene detects, the product after the related molecular marker InDel-Rf1a amplification of Restore gene detects with 3% sepharose.Observe in gel imaging instrument with or without polymorphic and take a picture preserve.
6. method according to claim 1, is characterized in that: step 1 moderate purity statistics is as follows: scent gene stripe size is 209bp, and non-scent gene stripe size is 201bp; Restore gene stripe size is 1145bp, and non-recovery gene stripe size is 575bp.Both carry out band reading respectively, consider and carry out purity calculating.Real cross-fertilize seed is scent gene and Restore gene is all heterozygosis band; Restorer is that Restore gene isozygotys, British plain spirits gene; Sterile line is without Restore gene, and scent gene isozygotys; Then be divided into hybrid strain as there are other band situations or be likely that Parent seed production purity causes not.
Superiority of the present invention is:
The invention solves traditional field planting and detect the impact time-consuming, detected result is subject to environment, more early can predict purity of hybrid, be convenient to arrange further work plan.This cover scent gene and Restore gene molecular function mark select according to the Parent feature of cenospecies, application also has advantage that is quick, easy, low cost, is suitable for the application of the aspect such as Testing and appraisal, molecular mark of the grand excellent 619 seed purity real-time analyses of cross-fertilize seed of large sample, fake and forged kind.
Accompanying drawing explanation
The detected result that Fig. 1 utilizes the related molecular marker GRFM04 of scent gene to increase
The detected result that Fig. 2 utilizes the related molecular marker InDel-Rf1a of Restore gene to increase
Embodiment
1. a technological method for grand excellent 619 purity of rapid detection north Quality Hybrid Japonica Rice, is characterized in that:
Northern Quality Hybrid Japonica Rice grand excellent 619 for results carries out presoaking and germinating, DNA extraction, pcr amplification, electrophoresis detection and analysis, finally adds up purity of hybrid.
6. method according to claim 1, is characterized in that: have randomness when the northern Quality Hybrid Japonica Rice of results grand excellent 619 is soaked seed in step 1, and seed moisture content controls about 14%, meets field planting rule.
7. method according to claim 1, is characterized in that: in step 1 during presoaking and germinating, and 50 ~ 100 seeds selected by each culture dish, add clear water 15ml, 25 ~ 28 DEG C about illumination cultivation 10-15 days, treat that seedling grows to the 3-4 leaf phase and starts sampling.Have randomness during sampling, do not distinguish seedling size, power, the root of each whole plant, stem, leaf put into a 2ml centrifuge tube, and sampled population can experimentally conditional decision, more than at least 100 strains, in order to DNA extraction.
8. method according to claim 1, it is characterized in that: DNA extraction in step 1, put into the plain ball that diameter 2.75mm and 4.75mm varies in size in the 2ml centrifuge tube of application of sample, add 800 μ l SDS extracting solutions, high-throughput tissue grinder grinds, and frequency is 55HZ, the time is 180S.Grind rear 65 DEG C of water-baths about 30 minutes.Add 800 μ l phenol in centrifuge tube after water rain: chloroform: primary isoamyl alcohol 25: 24: 1, volume ratio, fully mixes, the centrifugal 10min of desk centrifuge 10000rpm, gets supernatant 500 μ l and is transferred to another 1.5ml centrifuge tube.Then carry out second time extracting, add 500 μ l phenol: chloroform: primary isoamyl alcohol 25: 24: 1, volume ratio, fully mixes, the centrifugal 10min of desk centrifuge 10000rpm, gets supernatant 400 μ l and is transferred to another 1.5ml centrifuge tube.Add 320 μ l Virahols, mixing, 4 DEG C or place 20min on ice, the centrifugal 10min of 10000rpm, outwells supernatant liquor, centrifuge tube is inverted about 10-15min and is filtered dry alcohol, and then DNA is dissolved in 100 μ l distilled waters ,-20 DEG C save backup.DNA solution dilutes 15 times, for pcr amplification.
9. method according to claim 1, is characterized in that: in step 1 during pcr amplification, selects molecule marker according to the grand breediness of excellent 619.Through gene test, grand excellent 619 female parents are containing scent gene fgr, male parent contains Restoring gene Rf 1 a, so select the related molecular marker GRFM04 (F:3 '-GTTAGGTTGCATTTACTGGGAG-5 ' of scent gene, R:3 '-GAATGATGCTCAAAGTGTCT-5 ') and the related molecular marker InDel-Rf1a (F:3 '-CTGATGATCGAGGAGGAGGTA-5 ', R:3 '-TAACGCGTCTTCCATCCTACT-5 ') of Restore gene carry out pcr amplification.
(1) amplification reaction system is as follows:
(2) amplification reaction condition
The related molecular marker GRFM04 amplification reaction condition of scent gene is as follows:
94 DEG C of denaturation 5min; 94 DEG C of 1min, 55 DEG C of 1min, 72 DEG C of 2min, totally 35 circulations; Finally carry out 72 DEG C and extend 5min.4 DEG C of preservations are stand-by.
The related molecular marker InDel-Rf1a amplification reaction condition of Restore gene is as follows:
94 DEG C of denaturation 5min; 94 DEG C of 30s, 55 DEG C of 30s, 72 DEG C of 1min, totally 35 circulations; Finally carry out 72 DEG C and extend 7min.4 DEG C of preservations are stand-by.
(3) electrophoresis detection: the product polyacrylamide gel electrophoresis after the related molecular marker GRFM04 amplification of scent gene detects, the product after the related molecular marker InDel-Rf1a amplification of Restore gene detects with 3% sepharose.Observe in gel imaging instrument with or without polymorphic and take a picture preserve.
6. method according to claim 1, is characterized in that: step 1 moderate purity statistics is as follows: scent gene stripe size is 209bp, and non-scent gene stripe size is 201bp; Restore gene stripe size is 1145bp, and non-recovery gene stripe size is 575bp.Both carry out band reading respectively, consider and carry out purity calculating.Real cross-fertilize seed is scent gene and Restore gene is all heterozygosis band; Restorer is that Restore gene isozygotys, British plain spirits gene; Sterile line is without Restore gene, and scent gene isozygotys; Then be divided into hybrid strain as there are other band situations or be likely that Parent seed production purity causes not.
Claims (6)
1. a technological method for grand excellent 619 purity of rapid detection north Quality Hybrid Japonica Rice, is characterized in that:
Northern Quality Hybrid Japonica Rice grand excellent 619 for results carries out presoaking and germinating, DNA extraction, pcr amplification, electrophoresis detection and analysis, finally adds up purity of hybrid.
2. method according to claim 1, is characterized in that: have randomness when the northern Quality Hybrid Japonica Rice of results grand excellent 619 is soaked seed in step 1, and seed moisture content controls about 14%, meets field planting rule.
3. method according to claim 1, is characterized in that: in step 1 during presoaking and germinating, and 50 ~ 100 seeds selected by each culture dish, add clear water 15ml, 25 ~ 28 DEG C about illumination cultivation 10-15 days, treat that seedling grows to the 3-4 leaf phase and starts sampling.Have randomness during sampling, do not distinguish seedling size, power, the root of each whole plant, stem, leaf put into a 2ml centrifuge tube, and sampled population can experimentally conditional decision, more than at least 100 strains, in order to DNA extraction.
4. method according to claim 1, it is characterized in that: DNA extraction in step 1, put into the plain ball that diameter 2.75mm and 4.75mm varies in size in the 2ml centrifuge tube of application of sample, add 800 μ l SDS extracting solutions, high-throughput tissue grinder grinds, and frequency is 55HZ, the time is 180S.Grind rear 65 DEG C of water-baths about 30 minutes.Add 800 μ l phenol in centrifuge tube after water rain: chloroform: primary isoamyl alcohol 25: 24: 1, volume ratio, fully mixes, the centrifugal 10min of desk centrifuge 10000rpm, gets supernatant 500 μ l and is transferred to another 1.5ml centrifuge tube.Then carry out second time extracting, add 500 μ l phenol: chloroform: primary isoamyl alcohol 25: 24: 1, volume ratio, fully mixes, the centrifugal 10min of desk centrifuge 10000rpm, gets supernatant 400 μ l and is transferred to another 1.5ml centrifuge tube.Add 320 μ l Virahols, mixing, 4 DEG C or place 20min on ice, the centrifugal 10min of 10000rpm, outwells supernatant liquor, centrifuge tube is inverted about 10-15min and is filtered dry alcohol, and then DNA is dissolved in 100 μ l distilled waters ,-20 DEG C save backup.DNA solution dilutes 15 times, for pcr amplification.
5. method according to claim 1, is characterized in that: in step 1 during pcr amplification, selects molecule marker according to the grand breediness of excellent 619.Through gene test, grand excellent 619 female parents are containing scent gene fgr, male parent contains Restoring gene Rf 1 a, so select the related molecular marker GRFM04 (F:3 '-GTTAGGTTGCATTTACTGGGAG-5 ' of scent gene, R:3 '-GAATGATGCTCAAAGTGTCT-5 ') and the related molecular marker InDel-Rf1a (F:3 '-CTGATGATCGAGGAGGAGGTA-5 ', R:3 '-TAACGCGTCTTCCATCCTACT-5 ') of Restore gene carry out pcr amplification.
(1) amplification reaction system is as follows:
(2) amplification reaction condition
The related molecular marker GRFM04 amplification reaction condition of scent gene is as follows:
94 DEG C of denaturation 5min; 94 DEG C of 1min, 55 DEG C of 1min, 72 DEG C of 2min, totally 35 circulations; Finally carry out 72 DEG C and extend 5min.4 DEG C of preservations are stand-by.
The related molecular marker InDel-Rf1a amplification reaction condition of Restore gene is as follows:
94 DEG C of denaturation 5min; 94 DEG C of 30s, 55 DEG C of 30s, 72 DEG C of 1min, totally 35 circulations; Finally carry out 72 DEG C and extend 7min.4 DEG C of preservations are stand-by.
(3) electrophoresis detection: the product polyacrylamide gel electrophoresis after the related molecular marker GRFM04 amplification of scent gene detects, the product after the related molecular marker InDel-Rf1a amplification of Restore gene detects with 3% sepharose.Observe in gel imaging instrument with or without polymorphic and take a picture preserve.
6. method according to claim 1, is characterized in that: step 1 moderate purity statistics is as follows: scent gene stripe size is 209bp, and non-scent gene stripe size is 201bp; Restore gene stripe size is 1145bp, and non-recovery gene stripe size is 575bp.Both carry out band reading respectively, consider and carry out purity calculating.Real cross-fertilize seed is scent gene and Restore gene is all heterozygosis band; Restorer is that Restore gene isozygotys, British plain spirits gene; Sterile line is without Restore gene, and scent gene isozygotys; Then be divided into hybrid strain as there are other band situations or be likely that Parent seed production purity causes not.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107699632A (en) * | 2017-11-20 | 2018-02-16 | 安徽省农业科学院水稻研究所 | InDel marks, primer and the application of analyzing rice genetic diversity, identification of species |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1944668A (en) * | 2006-08-03 | 2007-04-11 | 武汉大学 | Molecular labeling method for identifying hybrid rice Honglian seed |
| CN102550346A (en) * | 2010-12-28 | 2012-07-11 | 天津天隆农业科技有限公司 | Method for increasing yield of Longyou 619 hybrid seed of three-line japonica hybrid rice |
-
2014
- 2014-12-30 CN CN201410837719.0A patent/CN104450944A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1944668A (en) * | 2006-08-03 | 2007-04-11 | 武汉大学 | Molecular labeling method for identifying hybrid rice Honglian seed |
| CN102550346A (en) * | 2010-12-28 | 2012-07-11 | 天津天隆农业科技有限公司 | Method for increasing yield of Longyou 619 hybrid seed of three-line japonica hybrid rice |
Non-Patent Citations (4)
| Title |
|---|
| 国家水稻数据中心: "新品种权申请公告号CNA008478E", 《中国水稻品种及其系谱数据库》 * |
| 杂交水稻产业技术创新联盟: "杂交水稻产业技术创新战略联盟2013年工作总结及2014年工作展望", 《杂交水稻产业技术创新战略联盟2013年工作总结及2014年工作展望》 * |
| 王丰等: "一种水稻香味基因功能标记的开发", 《中国水稻科学》 * |
| 陈涛等: "粳稻BT型细胞质雄性不育恢复基因功能标记的开发与应用", 《中国水稻科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107699632A (en) * | 2017-11-20 | 2018-02-16 | 安徽省农业科学院水稻研究所 | InDel marks, primer and the application of analyzing rice genetic diversity, identification of species |
| CN107699632B (en) * | 2017-11-20 | 2021-06-11 | 安徽省农业科学院水稻研究所 | InDel marker for analyzing genetic diversity of rice and identifying variety, primer and application |
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