CN104450823B

CN104450823B - A kind of method for improving nimoctin fermentation yield

Info

Publication number: CN104450823B
Application number: CN201410787858.7A
Authority: CN
Inventors: 李明; 杨丽锋
Original assignee: HEBEI SHENGXUE DACHENG PHARMACEUTICAL CO Ltd
Current assignee: HEBEI SHENGXUE DACHENG PHARMACEUTICAL CO Ltd
Priority date: 2014-12-18
Filing date: 2014-12-18
Publication date: 2017-11-17
Anticipated expiration: 2034-12-18
Also published as: CN104450823A

Abstract

A kind of method for improving nimoctin fermentation yield, comprises the following steps：A, starting strain is chosen；B, bacterial strain inclined-plane culture；C, bacterial strain kind bottle culture；D, seed tank culture；E, fermentation tank culture.The method of a kind of raising nimoctin fermentation yield of the present invention, by fermentation middle and later periods single or multiple significantly liftings added sodium acetate, realize nimoctin yield.

Description

A kind of method for improving nimoctin fermentation yield

Technical field

The present invention relates to the fermenting and producing of nimoctin, and in particular to a kind of method for improving nimoctin fermentation yield.

Background technology

Nimoctin is a kind of 16-membered ring macrolides as caused by streptomyces chromogenes or cyaneogriseus streptomyces fermentation Antibiotic, it is mainly used in synthesizing the stronger moxidectin of lipophilicity.Nimoctin is mainly used in the stronger agricultural chemicals of synthesizing activity Veterinary drug moxidectin (Moxidectin).Used since moxidectin mid-term the 1980s initially as anthelmintic for animals, Nematode and epizoon can be efficiently killed, while has good security to animal, is to be currently widely used for animal doctor The wide spectrum of clinic, efficient, Novel macrocyclic lactone expelling parasite antibiotic.

Nimoctin yields poorly in the prior art, far can not meet industrial requirement.Buddhist nun's Mack is improved in the prior art The method in spit of fland, which has, to be chosen the bacterial strain of high yield, mends sugar, addition amino acid or other nutritional ingredients etc., for example, Application No. 201110242857.0 patent《A kind of method of fermenting and producing nimoctin》In disclose " in fermenting and producing nimoctin During, when the total sugar concentration in zymotic fluid is less than 30g/L, by intermittently or serially adding glucide to zymotic fluid, make Total sugar concentration control in zymotic fluid is fermented in 10-40g/L ", nimoctin is improved by gap or continuous benefit sugar Yield, but its effect is still undesirable, glucose metabolism reptation behavior during the fermentation be present, and glucose is to Buddhist nun's Mack The yield in spit of fland has critical influence, thus, although nimoctin yield can be improved by mending sugar, have necessarily Limitation.

The content of the invention

The technology that the present invention has much room for improvement for yield when solving the yielding poorly of nimoctin, fermentation ends in the prior art Problem, there is provided utilize a kind of method that can improve nimoctin fermentation yield.

The present invention is to realize that the technical scheme that its purpose uses is：

A kind of method for improving nimoctin fermentation yield, it is characterised in that comprise the following steps：

A, starting strain is chosen：One plant of sodium acetate resistance streptomyces chromogenes mutant strain is chosen, described one plant of sodium acetate resists The specific name of property streptomyces chromogenes mutant strain is streptomyces chromogenes (Streptomyces cyanneorisens) DC18-01, China Committee for Culture Collection of Microorganisms is deposited in, deposit number is CGMCC No.6217, and preservation date is 2012 6 The moon 14；

B, bacterial strain inclined-plane culture：Streptomyces chromogenes (Streptomyces cyanneorisens) DC18-01 is inoculated in Slant medium, 96-144h is cultivated under 26-30 DEG C, 30%-60% damp conditions, obtains slant strains；

C, bacterial strain kind bottle culture：Take 2-5cm²Slant strains prepared by step B are inoculated in kind of bottle culture medium, in 26- 30 DEG C, 220-240rpm concussion and cultivate 24-30h, obtain ripe kind liquid；

D, seed tank culture：The ripe kind liquid that step C is obtained is seeded to seeding tank training with the inoculum concentration of volume ratio 0.05% Support in base, it is 27.5-28.5 DEG C control cultivation temperature, and tank pressure is 0.03-0.05MPa, and the dissolved oxygen in incubation, which controls, to exist More than 25%, obtain kind of a bottle nutrient solution；

E, fermentation tank culture：The kind bottle nutrient solution that step D is obtained is seeded to fermented and cultured with the inoculum concentration of volume ratio 10% In base, it is 27.5-28.5 DEG C to control fermentation jar temperature, and tank pressure be 0.03-0.05MPa, and the dissolved oxygen control in zymotic fluid is 60% More than, in 0.5-2.0g/mL, after the 150h that ferments concentration is added into zymotic fluid at least once is for concentration of glucose control 10-20mg/L sodium acetate solution, the sodium acetate of 1-2% volume ratios is added altogether, stop mending sugar in fermentation 260-280h, work as grape Sugared concentration stops fermentation when being less than 0.2g/mL, and the zymotic fluid containing nimoctin is made.

168h fills into sodium acetate solution after fermentation starts during described step E fermentation tank cultures.

168h, 216h fill into sodium acetate solution after fermentation starts during described step E fermentation tank cultures.

156h, 192h and 228h fill into sodium acetate solution after fermentation starts during described step E fermentation tank cultures.

Inclined-plane culture based component and dosage (g/100mL) described in step B are：Glucose 0.3-0.5, maltose 0.1- 0.15th, yeast extract or dusty yeast 0.3-0.5, starch 0.4-0.6, soybean cake powder 0.4-0.6, dextrin 0.4-0.6, dipotassium hydrogen phosphate 0.01-0.03, agar 1-2.

Kind bottle medium component and dosage (g/100mL) described in step C are：Glucose 0.5-2, dextrin 1-3, soyabean cake Powder 1-2, yeast extract or dusty yeast 1-2, magnesium sulfate 0.05-0.2, dipotassium hydrogen phosphate 0.05-0.2.

Seed tank culture based component and dosage (g/100mL) described in step D are：Glucose 2-3, soybean cake powder 1-2, ferment Female cream or dusty yeast 1-2, magnesium sulfate 0.05-0.2, dipotassium hydrogen phosphate 0.05-0.2.

The composition and dosage (g/100mL) of fermentation medium described in step E be：Glucose 4-6, soybean cake powder 2.5- 3.5, dusty yeast 0.3-0.5, magnesium sulfate 0.8-1.2, calcium carbonate 0.2-0.4.

The beneficial effects of the invention are as follows：The method of a kind of raising nimoctin fermentation yield of the present invention, by fermenting Middle and later periods single or multiple significantly liftings added sodium acetate, realize nimoctin yield.In the course of the study, face How to overcome acetic acid sodium content in zymotic fluid high and synthetase series to nimoctin when producing the difficulty of inhibitory action, it is therein Puzzlement is unthinkable, and we have passed through long-term creative research, overcomes a variety of difficulties, this process be it is difficult still Development of the research of its successful to nimoctin is significant.

From the composition principle of nimoctin, a kind of precursor substance of the sodium acetate as nimoctin, its addition pair The yield of nimoctin plays critical effect, and sodium acetate produces NaOH after being utilized by mycelium, raises zymotic fluid pH value, And the too high growth not only bad for thalline of zymotic fluid pH value, and suppress the synthesis of nimoctin, from Fig. 1 prior art acetic acid Sodium show in the research to nimoctin yield effect rule sodium acetate addition from 0-0.2% when, nimoctin sustained production Rise, when sodium acetate addition is more than 0.2%, cell concentration declines, and the yield of nimoctin also drastically declines, and reason is Sodium acetate concentration rise produces inhibitory action to the synthetase series of nimoctin in zymotic fluid.And we are by long-term research, Break the normal procedure, increase the addition of sodium acetate, originally think can because the sodium acetate of high addition to the synthetase series of nimoctin Inhibitory action is produced, unexpected, the yield of nimoctin does not decline not only, has significantly lifted on the contrary, overcome Prior art sodium acetate addition is more than the technology prejudice that the yield of 0.2% nimoctin drastically declines.

Brief description of the drawings

Fig. 1 is affecting laws of the sodium acetate to nimoctin yield

Embodiment

The technology that the present invention has much room for improvement for yield when solving the yielding poorly of nimoctin, fermentation ends in the prior art Problem, there is provided utilize a kind of method that can improve nimoctin fermentation yield.The present invention is made below by specific embodiment Further instruction.

Sodium acetate is not added during embodiment 1.1.5T ferment tanks

E, fermentation tank culture：0.9T fermentation mediums are configured in 1.5T fermentation tanks, are cooled to after 120 DEG C of sterilizing 20min 27.5-28.5 DEG C, the kind bottle nutrient solution that step D is obtained is seeded in fermentation medium with the inoculum concentration of volume ratio 10%, controlled Fermentation jar temperature is 27.5-28.5 DEG C, and tank pressure be 0.03-0.05MPa, and dissolved oxygen is controlled more than 60% in fermentation process, grape Sugared concentration is controlled in 0.5-2.0g/mL, stops mending sugar in fermentation 260h, the stopping fermentation when concentration of glucose is less than 0.2g/mL, The zymotic fluid containing nimoctin is made, it is 2560mg/L to determine last fermentation yield.

Fermentation starts rear 168h and adds sodium acetate during embodiment 2.1.5T ferment tanks

E, fermentation tank culture：0.9T fermentation mediums are configured in 1.5T fermentation tanks, are cooled to after 120 DEG C of sterilizing 20min 27.5-28.5 DEG C, the kind bottle nutrient solution that step D is obtained is seeded in fermentation medium with the inoculum concentration of volume ratio 10%, controlled Fermentation jar temperature is 27.5-28.5 DEG C, and tank pressure be 0.03-0.05MPa, and dissolved oxygen is controlled more than 60% in fermentation process, grape Sugared concentration is controlled in 0.5-2.0g/mL, and 168h fills into the sodium acetate solution that 10L concentration is 10mg/L after fermentation starts, and is sending out Ferment 260h stops mending sugar, stops fermentation when concentration of glucose is less than 0.2g/mL, and the zymotic fluid containing nimoctin is made, and surveys Fixed last fermentation yield is 2980mg/L.

Fermentation starts rear 168h, 216h and adds sodium acetate A, selects bacterium germination during embodiment 3.12T ferment tanks Strain：One plant of sodium acetate resistance streptomyces chromogenes mutant strain is chosen, described one plant of sodium acetate resistance streptomyces chromogenes mutant strain Specific name is streptomyces chromogenes (Streptomyces cyanneorisens) DC18-01, is deposited in Chinese microorganism strain Preservation administration committee, deposit number are CGMCC No.6217, and preservation date is on June 14th, 2012；

E, fermentation tank culture：7.5T fermentation mediums are configured in 12T fermentation tanks, are cooled to after 120 DEG C of sterilizing 20min 27.5-28.5 DEG C, the kind bottle nutrient solution that step D is obtained is seeded in fermentation medium with the inoculum concentration of volume ratio 10%, controlled Fermentation jar temperature is 27.5-28.5 DEG C, and tank pressure be 0.03-0.05MPa, and dissolved oxygen is controlled more than 60% in fermentation process, grape Sugared concentration is controlled in 0.5-2.0g/mL, and it is 15mg/L to fill into 50L and 70L concentration respectively in 168h and 216h after fermentation starts Sodium acetate solution, stop mending sugar in fermentation 276h, stop fermentation when concentration of glucose be less than 0.2g/mL, it is obtained to contain Buddhist nun The zymotic fluid in Mack spit of fland, it is 3343mg/L to determine last fermentation yield.

Fermentation starts rear 156h, 192h and 228h and adds sodium acetate during embodiment 4.12T ferment tanks

E, fermentation tank culture：7.5T fermentation mediums are configured in 12T fermentation tanks, are cooled to after 120 DEG C of sterilizing 20min 27.5-28.5 DEG C, the kind bottle nutrient solution that step D is obtained is seeded in fermentation medium with the inoculum concentration of volume ratio 10%, controlled Fermentation jar temperature is 27.5-28.5 DEG C, and tank pressure be 0.03-0.05MPa, and dissolved oxygen is controlled more than 60% in fermentation process, grape Sugared concentration is controlled in 0.5-2.0g/mL, and 60L, 40L and 60L concentration are filled into respectively in 156h, 192h and 228h after fermentation starts For 20mg/L sodium acetate solution, stop mending sugar in fermentation 264h, stop fermentation, system when concentration of glucose is less than 0.2g/mL Must be containing the zymotic fluid of nimoctin, it is 3690mg/L to determine last fermentation yield.

Claims

A kind of 1. method for improving nimoctin fermentation yield, it is characterised in that comprise the following steps：

A, starting strain is chosen：Choose one plant of sodium acetate resistance streptomyces chromogenes mutant strain, described one plant of sodium acetate resistance production The specific name of color streptomycete mutant strain is streptomyces chromogenes (Streptomyces cyanneorisens) DC18-01, preservation In China Committee for Culture Collection of Microorganisms, deposit number is CGMCC No.6217, and preservation date is June 14 in 2012 Day；

B, bacterial strain inclined-plane culture：Streptomyces chromogenes (Streptomyces cyanneorisens) DC18-01 is inoculated in inclined-plane Culture medium, 96-144h is cultivated under 26-30 DEG C, 30%-60% damp conditions, obtains slant strains；

C, bacterial strain kind bottle culture：Take 2-5cm²Slant strains prepared by step B are inoculated in kind of bottle culture medium, in 26-30 DEG C, 220-240rpm concussion and cultivate 24-30h, obtain ripe kind liquid；

D, seed tank culture：The ripe kind liquid that step C is obtained is seeded to seed tank culture base with the inoculum concentration of volume ratio 0.05% In, it is 27.5-28.5 DEG C to control cultivation temperature, and tank pressure be 0.03-0.05MPa, the dissolved oxygen in incubation control 25% with On, obtain kind of a bottle nutrient solution；

E, fermentation tank culture：The kind bottle nutrient solution that step D is obtained is seeded in fermentation medium with the inoculum concentration of volume ratio 10%, It is 27.5-28.5 DEG C to control fermentation jar temperature, and tank pressure be 0.03-0.05MPa, the dissolved oxygen control in zymotic fluid more than 60%, Concentration of glucose is controlled in 0.5-2.0g/mL, and it is 10- to add concentration into zymotic fluid at least once after the 150h that ferments 20mg/L sodium acetate solution, the sodium acetate of 1-2% volume ratios is added altogether, stop mending sugar in fermentation 260-280h, work as glucose Concentration stops fermentation when being less than 0.2g/mL, and the zymotic fluid containing nimoctin is made.
A kind of 2. method for improving nimoctin fermentation yield according to claim 1, it is characterised in that：Described step 168h fills into sodium acetate solution after fermentation starts during E fermentation tank cultures.
A kind of 3. method for improving nimoctin fermentation yield according to claim 1, it is characterised in that：Described step 168h, 216h fill into sodium acetate solution after fermentation starts during E fermentation tank cultures.
A kind of 4. method for improving nimoctin fermentation yield according to claim 1, it is characterised in that：Described step 156h, 192h and 228h fill into sodium acetate solution after fermentation starts during E fermentation tank cultures.
A kind of 5. method for improving nimoctin fermentation yield according to claim 1, it is characterised in that：Described in step B Inclined-plane culture based component and dosage be calculated as by g/100mL：Glucose 0.3-0.5, maltose 0.1-0.15, yeast extract or yeast Powder 0.3-0.5, starch 0.4-0.6, soybean cake powder 0.4-0.6, dextrin 0.4-0.6, dipotassium hydrogen phosphate 0.01-0.03, agar 1- 2。
A kind of 6. method for improving nimoctin fermentation yield according to claim 1, it is characterised in that：Described in step C Kind bottle medium component and dosage be calculated as by g/100mL：Glucose 0.5-2, dextrin 1-3, soybean cake powder 1-2, yeast extract or Dusty yeast 1-2, magnesium sulfate 0.05-0.2, dipotassium hydrogen phosphate 0.05-0.2.
A kind of 7. method for improving nimoctin fermentation yield according to claim 1, it is characterised in that：Described in step D Seed tank culture based component and dosage be calculated as by g/100mL：Glucose 2-3, soybean cake powder 1-2, yeast extract or dusty yeast 1- 2nd, magnesium sulfate 0.05-0.2, dipotassium hydrogen phosphate 0.05-0.2.
A kind of 8. method for improving nimoctin fermentation yield according to claim 1, it is characterised in that：Described in step E Fermentation medium composition and dosage be calculated as by g/100mL：Glucose 4-6, soybean cake powder 2.5-3.5, dusty yeast 0.3- 0.5, magnesium sulfate 0.8-1.2, calcium carbonate 0.2-0.4.