CN104450500B - Magnetic stirrer applied to disposable bag type bioreactor - Google Patents
Magnetic stirrer applied to disposable bag type bioreactor Download PDFInfo
- Publication number
- CN104450500B CN104450500B CN201410616553.XA CN201410616553A CN104450500B CN 104450500 B CN104450500 B CN 104450500B CN 201410616553 A CN201410616553 A CN 201410616553A CN 104450500 B CN104450500 B CN 104450500B
- Authority
- CN
- China
- Prior art keywords
- magnetic assembly
- radial center
- blind hole
- separation sleeve
- type bioreactor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
Abstract
The invention discloses a magnetic stirrer applied to a disposable bag type bioreactor. The magnetic stirrer comprises an inner magnetic component, an inner magnetic fixing rod, an isolating sleeve, an outer magnetic component, a motor base and a motor which are sequentially coaxially arranged from top to bottom, wherein the outer magnetic component is driven to automatically rotate by the motor; the inner magnetic component automatically rotates by taking the inner magnetic fixing rod as the center under the driving of the outer magnetic component; the inner magnetic fixing rod and the isolating sleeve are coaxially fixed; a radial center of the top surface of the outer magnetic component is provided with an isolating sleeve blind hole coaxial to the radial center; the radial center of the bottom surface of the isolating sleeve blind hole is provided with a convex arc surface coaxial to the radial center; the isolating sleeve comprises an isolating sleeve seat; the radial center of the top surface of the isolating sleeve seat is provided with a cylindrical boss which can be coaxially sleeved with the isolating sleeve blind hole; the radial center of the bottom surface of the cylindrical boss is provided with an upper concave arc surface coaxial to the radial center; the cylindrical boss extends into the isolating sleeve blind hole, so that the vortex of the upper concave arc surface is tangential to that of the upper convex arc surface.
Description
Technical field
The present invention relates to a kind of disposable pocket type bioreactor magnetic stirring apparatuss in biochemical equipment field.
Background technology
Disposable pocket type bioreactor has a wide range of applications in biochemistry, disposable pocket type bioreactor magnetic
Power agitator is the key equipment matching with disposable pocket type bioreactor.Disposable pocket type bioreactor magnetic at present
The shortcoming of power agitator essentially consists in: the rotation center of interior magnetic assembly often deviates vertical direction, interior magnetic group when therefore stirring
The motility of part rotation and rotating speed cannot be lifted, and its internal bearing easily damages, and affects its service life, disadvantages mentioned above is tight
Limit disposable pocket type bioreactor magnetic stirring apparatuss to use again, be the reason cause drawbacks described above its separation sleeve with
The way of contact between outer magnetic assembly is plane contact.
Content of the invention
The invention aims to overcoming the deficiencies in the prior art, provide a kind of disposable pocket type bioreactor magnetic
Power agitator, it can prevent the rotation center of interior magnetic assembly from deviateing vertical direction it is ensured that motility during interior magnetic assembly rotation
And rotating speed, its service life also extended.
A kind of technical scheme realizing above-mentioned purpose is: a kind of disposable pocket type bioreactor magnetic stirring apparatuss, bag
Include the sequentially coaxially interior magnetic assembly of setting, interior magnetically fixed bar, separation sleeve, outer magnetic assembly, motor cabinet and motor from top to bottom, described
Outer magnetic assembly rotation under the driving of described motor, described interior magnetic assembly is solid with described interior magnetic under the driving of described outer magnetic assembly
Rotation centered on fixed pole, described interior magnetically fixed bar is coaxially fixed with described separation sleeve；:
The radial center of described outer magnetic assembly top surface is provided with a separation sleeve blind hole being coaxial therewith, described separation sleeve blind hole
The radial center of bottom surface be provided with its coaxial upper convex globoidal,
Described separation sleeve includes an isolation block set, the radial center of described isolation block set top surface be provided with one can with institute every
From the cylinder boss of the coaxial socket of set blind hole, the radial center of the bottom surface of described cylinder boss is provided with the upper concave arc being coaxial therewith
Face；
Described cylinder boss stretches in described separation sleeve blind hole, makes the summit of described upper cancave cambered surface and described upper convex globoidal
Summit is tangent.
Further, described upper cancave cambered surface is hemisphere face, half ellipsoidal surface, parabola or hyperboloid.
Further, described upper convex globoidal is hemisphere face, half ellipsoidal surface, parabola or hyperboloid.
Further, the external diameter of described cylinder boss is less than the internal diameter of described separation sleeve blind hole.
Further, described isolation block set bottom surface be provided with one coaxial with described cylinder boss, can be with described outer magnetic
The outer ring seat of assembly socket, the internal diameter of described outer ring seat is more than the external diameter of outer magnetic assembly.
Further, described interior magnetic assembly includes a shaft being located at its radial center, the top of described shaft
It is provided with some stirrer paddles around its circumference uniform distribution.
Further, described interior magnetic assembly and described interior magnetically fixed bar by positioned at its radially between bearing, and
The bearing positioning sleeve of the fixing described bearing of in the vertical direction is fixed, and passes through spiral shell between described interior magnetically fixed bar and described separation sleeve
Stricture of vagina is fixed.
Further, it is provided with the internal magnet around its circumference uniform distribution in described interior magnetic assembly, be provided with described outer magnetic assembly
Around the external magnet of its circumference uniform distribution, described internal magnet and described external magnet in the vertical direction are one-to-one.
Employ the technical scheme of a kind of disposable pocket type bioreactor magnetic stirring apparatuss of the present invention, that is, in outer magnetic
The bottom surface of the separation sleeve blind hole of assembly increases a upper convex globoidal being coaxial therewith, in the radial center of the cylinder boss of separation sleeve
It is coaxially disposed cancave cambered surface on；Described cylinder boss stretches in described separation sleeve blind hole, makes summit and the institute of described upper cancave cambered surface
The summit stating convex globoidal is tangent.It has the technical effect that it can prevent the rotation center of interior magnetic assembly from deviateing vertical direction, protects
Motility during magnetic assembly rotation and rotating speed in card, the service life of disposable pocket type bioreactor magnetic stirring apparatuss also obtains
To extend.
Brief description
Fig. 1 is the sectional view of a kind of disposable pocket type bioreactor magnetic stirring apparatuss of the present invention.
Fig. 2 is the top view of a kind of disposable pocket type bioreactor magnetic stirring apparatuss of the present invention.
Fig. 3 is the structural representation of the outer magnetic assembly of a kind of disposable pocket type bioreactor magnetic stirring apparatuss of the present invention
Figure.
Fig. 4 is the structural representation of the separation sleeve of a kind of disposable pocket type bioreactor magnetic stirring apparatuss of the present invention
Figure.
Specific embodiment
Refer to Fig. 1 to Fig. 4, the present inventor in order to be able to preferably understand to technical scheme,
Below by specifically embodiment, and combine accompanying drawing and be described in detail:
Refer to Fig. 1, the disposable pocket type bioreactor magnetic stirring apparatuss of the present invention are included from top to bottom successively together
The solid push rod 6 of the interior magnetic assembly 1 of axle setting, interior magnetic, separation sleeve 4, outer magnetic assembly 5, motor cabinet 7 and motor 8.
Wherein interior magnetic assembly 1 includes shaft 11, the top of shaft 11 be provided with some around shaft 11 circumference uniform distribution
Stirrer paddle 12, shaft 11 stretches into inside disposable pocket type bioreactor, cell culture fluid is stirred.Shaft
11 bottom sets the interior magnetic support 13 coaxial with shaft 11, is provided with some pieces along interior magnetic support 13 circumference uniform distribution in interior magnetic support 13
Internal magnet 131.The bottom of shaft 11, i.e. the radial center of the bottom of interior magnetic assembly 1, it is provided with and be coaxially disposed with shaft 11
Interior magnetic blind hole 14.
Motor 8 is located at the bottom of this disposable pocket type bioreactor magnetic stirring apparatus, and the top of motor 8 is provided with one
The drive shaft 81 coaxial with shaft 11, motor fixing seat 7 is fixed on the top surface of motor 8.
Outer magnetic assembly 5 includes outer magnetic support 51 and is located at outer magnetic support 51 bottom surface, the driving being coaxially disposed with outer magnetic support 51
Axle sleeve 53.The radial center of propeller shaft sleeve 53 bottom surface is provided with a drive shaft blind hole 54 coaxial with propeller shaft sleeve 53.Drive shaft
Set 53 is socketed in the drive shaft 81 of motor fixing seat 7 radially inner side and motor 8 by drive shaft blind hole 54.The drive shaft of motor 8
Pass through keying fixed between 81 and the hole wall of drive shaft blind hole 54.It is provided with outer magnetic support 51 along outer magnetic support 51 circumference uniform distribution, with
Internal magnet 131 quantity identical external magnet 511 in interior magnetic support 13.Every block of external magnet 511 in outer magnetic assembly 5 is all located at one piece
The underface of inner magnet 131.I.e. the internal magnet 131 in the external magnet 511 and interior magnetic assembly 1 in outer magnetic assembly 5 is in vertical direction
On be one-to-one.Outer magnetic assembly 5 rotation under the driving of the drive shaft 81 of motor 8, so drive in magnetic assembly 1 from
Turn.The radial center of outer magnetic support 51 top surface, is provided with the separation sleeve blind hole 52 of a cylinder coaxial with outer magnetic support 51.
Separation sleeve 4 includes isolating block set 41, and the bottom surface of isolation block set 41 is provided with outer ring seat 42, and is located at outer ring seat 42
Radially inner side, i.e. the cylinder boss 43 of the radial center of block set 41 bottom surface, isolation block set 41, outer ring seat 42 and cylinder boss 43
It is coaxially disposed.Cylinder boss 43 stretches in the separation sleeve blind hole 52 of radial center of outer magnetic support 51 top surface, 52 sets of outer ring seat
It is connected on the radial outside of outer magnetic assembly 5, i.e. the radial outside of outer magnetic support 51.Separation sleeve 4 by outer ring seat 42 and cylinder boss 43,
Radially fixed with outer magnetic assembly 5.The radial center of isolation block set 41 top surface, that is, the radial center of separation sleeve 4 top surface, is provided with one
The tapped blind hole 44 coaxial with isolation block set 41.
Interior magnetically fixed bar 6 includes from top to bottom the upper fix bar 61 of sequentially coaxially setting, fixed rod seat 62 and lower fix bar
63.Upper fix bar 61 is socketed in the shaft blind hole 14 of interior magnetic assembly 1.The hole wall of upper fix bar 61 and shaft blind hole 14 it
Between be provided with bearing 2 and in the vertical direction by bearing positioning sleeve 3 fixing for bearing 2.The bottom surface of fixed rod seat 62 and isolation
The top surface of set 4, i.e. the top surface contact of isolation block set 41, thus interior magnetically fixed bar 6 in the vertical direction is fixed.Lower fix bar 63
In the tapped blind hole 44 of the radial center stretching into separation sleeve 4 top surface, interior magnetically fixed bar 6 is made to pass through screw thread and isolation in vertical direction
Set 4 is fixing, and determines the rotation center of interior magnetic assembly 1.
Maximum being improved by of the present invention: the radial center of separation sleeve blind hole 52 bottom surface is provided with one and separation sleeve blind hole 52
Coaxial upper convex globoidal 521, that is, the summit going up convex globoidal 521 is located at the radial center of separation sleeve blind hole 52.Cylinder boss 43 bottom
The center in face is provided with a upper cancave cambered surface 431 coaxial with cylinder boss 43, that is, the radial center going up cancave cambered surface 431 is located at cylinder
In the radial center of boss 43.The summit of the summit of upper cancave cambered surface 431 and upper convex globoidal 521 is tangent.
The purpose of so design is: compared to traditional separation sleeve blind hole 52 bottom surface directly and cylinder boss 43 bottom
Face contacts, or the top surface of outer magnetic support 51, the design that is, top surface of outer magnetic assembly 5 is directly contacted with isolation block set 41 bottom surface, adopts
Summit with the summit of the upper cancave cambered surface 431 on cylinder boss 43 and the upper convex globoidal 521 of separation sleeve blind hole 52 bottom surface is tangent
Technical scheme, can prevent bottom surface due to separation sleeve blind hole 52, the bottom surface of cylinder boss 43, the top surface of outer magnetic support 51, isolation
The mismachining tolerance of block set 41 bottom surface and lead to the rotation center of the shaft 11 of interior magnetic assembly 1 to deviate vertical direction it is ensured that interior
Motility during magnetic assembly 1 rotation and rotating speed are it is ensured that the service life of disposable pocket type bioreactor magnetic stirring apparatuss.
The upper convex globoidal 521 of the upper cancave cambered surface 431 on cylinder boss 43 and separation sleeve blind hole 52 bottom surface can adopt following arc
Face: hemisphere face, half ellipsoidal surface, parabola, hyperboloid etc..
Summit in order to ensure the summit of upper cancave cambered surface 431 and upper convex globoidal 521 is tangent, and the internal diameter of outer ring seat 42 is more than outer
The external diameter of magnetic support 51, i.e. the external diameter of outer magnetic assembly 5.The external diameter of cylinder boss 43 is less than the internal diameter of separation sleeve blind hole 52.Outer magnetic support
51 top surface, that is, the top surface of outer magnetic assembly 5 is detached with isolation block set 41 bottom surface.The bottom surface of cylinder boss 43 is blind with separation sleeve
The bottom surface in hole 52 is also detached.
Such design, the length for the shaft 11 of interior magnetic assembly 1 is big, and stirrer paddle 12 pushes up positioned at shaft 11
The disposable pocket type bioreactor at end is especially suitable with magnetic stirring apparatuss, and noise during stirring is also obviously reduced.
In the present embodiment, upper convex globoidal 521 is the hemisphere face of a radius 2mm, and upper cancave cambered surface be radius is 3mm, deep
Hemisphere face for 1.5mm.
Those of ordinary skill in the art it should be appreciated that above embodiment be intended merely to illustrate the present invention,
And be not used as limitation of the invention, the change as long as in the spirit of the present invention, to embodiment described above
Change, modification all will fall in the range of claims of the present invention.
Claims (6)
1. a kind of disposable pocket type bioreactor magnetic stirring apparatuss, including the interior magnetic group of sequentially coaxially setting from top to bottom
Part, interior magnetically fixed bar, separation sleeve, outer magnetic assembly, motor cabinet and motor, described outer magnetic assembly under the driving of described motor from
Turn, the rotation centered on described interior magnetically fixed bar under the driving of described outer magnetic assembly of described interior magnetic assembly, described interior magnetically fixed
Bar is coaxially fixed with described separation sleeve；It is characterized in that:
The radial center of described outer magnetic assembly top surface is provided with a separation sleeve blind hole being coaxial therewith, the bottom of described separation sleeve blind hole
The radial center in face is provided with its coaxial upper convex globoidal,
Described separation sleeve includes an isolation block set, and the radial center of described isolation block set top surface is provided with one can be with institute's separation sleeve
The cylinder boss of the coaxial socket of blind hole, the radial center of the bottom surface of described cylinder boss is provided with the upper cancave cambered surface being coaxial therewith；
Described cylinder boss stretches in described separation sleeve blind hole, makes the summit of described upper cancave cambered surface and the summit of described upper convex globoidal
It is tangent,
The bottom surface of described isolation block set is provided with a, outer shroud that can with described outer magnetic assembly be socketed coaxial with described cylinder boss
Seat, the internal diameter of described outer ring seat is more than the external diameter of outer magnetic assembly,
It is provided with the internal magnet around its circumference uniform distribution in described interior magnetic assembly, be provided with around its circumference uniform distribution in described outer magnetic assembly
External magnet, described internal magnet and described external magnet in the vertical direction are one-to-one.
2. a kind of disposable pocket type bioreactor magnetic stirring apparatuss according to claim 1 it is characterised in that: described
Upper cancave cambered surface is hemisphere face, half ellipsoidal surface, parabola or hyperboloid.
3. a kind of disposable pocket type bioreactor magnetic stirring apparatuss according to claim 1 it is characterised in that: described
Upper convex globoidal is hemisphere face, half ellipsoidal surface, parabola or hyperboloid.
4. a kind of disposable pocket type bioreactor magnetic stirring apparatuss according to claim 1 it is characterised in that: described
The external diameter of cylinder boss is less than the internal diameter of described separation sleeve blind hole.
5. a kind of disposable pocket type bioreactor magnetic stirring apparatuss according to claim 1 it is characterised in that: described
Interior magnetic assembly includes a shaft being located at its radial center, the top of described shaft be provided with some around its circumference uniform distribution
Stirrer paddle.
6. a kind of disposable pocket type bioreactor magnetic stirring apparatuss according to claim 5 it is characterised in that: described
Interior magnetic assembly and described interior magnetically fixed bar are by the bearing between its radial direction, and in the vertical direction fixes described bearing
Bearing positioning sleeve fix, be threadably secured between described interior magnetically fixed bar and described separation sleeve.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410616553.XA CN104450500B (en) | 2014-11-05 | 2014-11-05 | Magnetic stirrer applied to disposable bag type bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410616553.XA CN104450500B (en) | 2014-11-05 | 2014-11-05 | Magnetic stirrer applied to disposable bag type bioreactor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104450500A CN104450500A (en) | 2015-03-25 |
| CN104450500B true CN104450500B (en) | 2017-01-18 |
Family
ID=52897259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410616553.XA Active CN104450500B (en) | 2014-11-05 | 2014-11-05 | Magnetic stirrer applied to disposable bag type bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104450500B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106388619B (en) * | 2016-10-08 | 2019-12-17 | 佛山市顺德区美的电热电器制造有限公司 | Upper cover with stirring function and electric cooking appliance |
| CN112980678A (en) * | 2021-02-04 | 2021-06-18 | 上海璟良生物工程技术有限公司 | Cylindrical surface coupling overhead type magnetic stirrer for disposable biological reaction bag |
| CN113897286B (en) * | 2021-10-11 | 2023-08-22 | 乐匀生物科技(南通)有限公司 | Disposable Bioreactor Stirring System |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201400670Y (en) * | 2009-03-27 | 2010-02-10 | 镇江科海生物工程设备有限公司 | Fermentor stirrer driven by magnetic coupling |
| CN201669067U (en) * | 2010-03-22 | 2010-12-15 | 刘怡然 | Stirring assembly |
| CN202179949U (en) * | 2011-06-20 | 2012-04-04 | 上海日泰医药设备工程有限公司 | Magnetic suspension stirrer |
| CN204107419U (en) * | 2014-11-05 | 2015-01-21 | 上海日泰医药设备工程有限公司 | A kind of disposable soft bag batching magnetic stirring apparatus |
-
2014
- 2014-11-05 CN CN201410616553.XA patent/CN104450500B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201400670Y (en) * | 2009-03-27 | 2010-02-10 | 镇江科海生物工程设备有限公司 | Fermentor stirrer driven by magnetic coupling |
| CN201669067U (en) * | 2010-03-22 | 2010-12-15 | 刘怡然 | Stirring assembly |
| CN202179949U (en) * | 2011-06-20 | 2012-04-04 | 上海日泰医药设备工程有限公司 | Magnetic suspension stirrer |
| CN204107419U (en) * | 2014-11-05 | 2015-01-21 | 上海日泰医药设备工程有限公司 | A kind of disposable soft bag batching magnetic stirring apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104450500A (en) | 2015-03-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104450500B (en) | Magnetic stirrer applied to disposable bag type bioreactor | |
| CN102711975B (en) | Device for stirring | |
| CN108889200A (en) | A kind of composite processing mixing device | |
| CN208233325U (en) | A kind of magnetic suspension propeller | |
| CN113144984A (en) | Magnetic stirring device and reaction kettle | |
| CN204107419U (en) | A kind of disposable soft bag batching magnetic stirring apparatus | |
| CN205056250U (en) | Flotation equipment | |
| CN107879463B (en) | Aeration tank | |
| CN111013469B (en) | Axle sleeve-free full-suspension magnetic stirrer | |
| CN108176316A (en) | A kind of lower magnetic suspension mixing plant | |
| CN104348297B (en) | A kind of magnetic coupling driving device | |
| CN202893268U (en) | Magnetic levitation stirrer | |
| CN207868183U (en) | Chuck pin and workpiece cleaner | |
| CN1287093C (en) | Liquid pump and sealing element | |
| CN204134534U (en) | The detachable agitating device of axle sleeve | |
| CN204841454U (en) | Reversible agitating unit | |
| CN205578288U (en) | Flowing water pump is made in oxygenation | |
| CN204768489U (en) | Gypsum agitated vessel | |
| CN102491547A (en) | Inverted-umbrella-type aeration impeller | |
| CN105538507A (en) | Material mixing synchronous stirrer for heat-preserving wallboard | |
| CN2870962Y (en) | Water powdery mixer | |
| CN107485316A (en) | One kind stirring cup | |
| CN202315744U (en) | Sanitary upward-magnetic-force-driven agitator | |
| CN215327112U (en) | Magnetic water activator capable of being repeatedly used | |
| CN203816522U (en) | Mushy feed stirring device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |