CN104448056A - Application of wheat AX (arabinoxylan) extracts to promoting DNA (deoxyribonucleic acid) transcription of intestinal tracts - Google Patents
Application of wheat AX (arabinoxylan) extracts to promoting DNA (deoxyribonucleic acid) transcription of intestinal tracts Download PDFInfo
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Abstract
The invention discloses an application of wheat AX (arabinoxylan) extracts to promoting DNA (deoxyribonucleic acid) transcription of intestinal tracts. The wheat AX extracts are prepared by a method comprising the following steps: carrying out centrifugal separation on industrial wastewater generated after extracting wheat gluten and/or wheat starch from wheat flour, and taking a supernatant; cooking the supernatant, and then carrying out centrifugal separation on the supernatant to obtain a supernatant; obtaining the wheat AX extracts by 1) carrying out spray drying on the supernatant, or 2) concentrating the supernatant, regulating the pH value to be 7-8, then adding beta-amylase and glucoamylase to react, finally obtaining a supernatant after centrifugal separation and precipitating the supernatant with ethanol. The wheat AX extracts also have the functions of improving the intestinal tract barrier functions, promoting growth of small intestinal villi, reducing the pH values of the intestinal tracts and increasing the contents of short-chain fatty acids in the intestinal tracts.
Description
Technical field
The present invention relates to the application of a kind of Wheat Arabinoxylan extract in promoting enteron aisle DNA to transcribe.
Background technology
Araboxylan (Arabinoxylans is called for short AX) is a kind of hemicellulose, is present in the nascent of plant and secondary cell wall, comprises cereal seed and timber.The polymkeric substance that AX is made up of wood sugar and pectinose two kinds sugar, also can be called piperylene.Basic structure comprises the xylan backbone and pectinose base side chain that xylose residues is formed by connecting through Isosorbide-5-Nitrae-glycosidic link, and some pectinose also also exists the phenolic acid be connected with ester bond.
The extraction of current Wheat Arabinoxylan mainly contains two kinds of sources, and one is extract from Testa Tritici, and one extracts from whole meal flour, and extracting method mainly contains alkaline extraction and enzyme formulation two kinds.
Wheat bran is the by product of wheat processing, arabinoxylan content enriches, about more than 20%, the annual wheat bran output of China, up to more than 2,000 ten thousand tons, is being extracted from wheat bran so the research of some araboxylan extraction processes at present focuses mostly on, but araboxylan great majority in wheat bran are all water insoluble, extract difficulty, method feasible is at present alkaline extraction, and alkali is put forward technique and can be brought a large amount of containing alkali waste water discharge, causes environmental pollution.
Arabinoxylan content in flour is about 2%, wherein the extractible part of water accounts for 0.5% ~ 0.8%, extracting directly araboxylan from flour, flour material consumption is large, and extraction cost is high, only carry out small-scale at present to extract in laboratory, extracting method mostly is the centrifugal cooperation enzyme process of physics to carry out, and only for scientific research, large-scale industrial production is explored not, do not form ripe operational path, can not industrialization production be carried out.
In the factory effluent of wheat starch, gluten powder, soluble arabinoxylan containing high-quality, China's whole meal flour secondary industry is huge, traditional wheat starch, gluten powder complete processing be by whole meal flour through water extraction with the operation such as to be separated and to obtain target compound, discard eluting water, often produce 1 ton of starch, the quantity discharged of high concentrated organic wastewater is between 5 ~ 12 tons, and the direct discharge of these organic waste waters causes severe contamination to environment.Domesticly at present also without any enterprise, these processing waste waters effectively to be utilized, analyze reason, on the one hand because starch, Gu Ruan powder enterprise do not recognize the utility value of wash-out waste water, the using value of araboxylan in physiologically active and processing characteristics is not understood, on the other hand, there is no efficient ripe separation-extraction technology at present, cannot be used to a large amount of wash-out waste water.
The domestic research about araboxylan physiologically active focuses mostly in the araboxylan in Testa Tritici source, and the araboxylan physiologically active extracted from wheat-flour rarely has report.Many investigators think that Testa Tritici may be the most effectively and the food fibre of the most applicable human body needs, food fibre composition more complicated in Testa Tritici, comprise Mierocrystalline cellulose, xylogen, dextran, araboxylan, arabogalactan etc., specifically any or several component plays a major role, and it be unclear that.
Summary of the invention
The object of this invention is to provide the application of a kind of Wheat Arabinoxylan extract in promoting enteron aisle DNA to transcribe, the Wheat Arabinoxylan extract used in the present invention, extract from the organic waste water of wheat starch processing enterprise, Gu Ruan powder processing enterprise, the discharge of gluten powder processing enterprise, therefore the wastewater discharge of above-mentioned processing enterprise can be reduced, realize energy-saving and emission-reduction, improve its added value of product.
Wheat Arabinoxylan extract provided by the invention promotes enteron aisle DNA to transcribe product in preparation or promotes the application in intestinal secretion metabolism product;
Described enteron aisle is specially duodenum;
Described Wheat Arabinoxylan extract can be prepared according to the method comprised the steps:
The factory effluent of wheat-flour after extracting gluten powder and/or wheat starch, through centrifugation, gets supernatant liquor; Described supernatant liquor carries out centrifugation again after boiling, obtains supernatant liquor; Described supernatant liquor is through following 1) or 2) step namely obtain Wheat Arabinoxylan extract:
1) described supernatant liquor is spray-dried and get final product;
2) described supernatant liquor is through concentrated, and adjusted to ph is after 7 ~ 8, adds beta-amylase and saccharifying enzyme reacts, and obtain supernatant liquor finally by after centrifugation, described supernatant liquor, after alcohol settling, to obtain final product.
In the present invention's application, described factory effluent can carry out centrifugation under the condition of 1000 revs/min ~ 3000 revs/min.
In the present invention's application, the temperature of described boiling can be 80 DEG C ~ 100 DEG C, and the time of described boiling can be 20 minutes ~ 70 minutes.
In the present invention's application, the described supernatant liquor after boiling carries out centrifugation under the condition of 2000 revs/min ~ 5000 revs/min.
In the present invention's application, step 1) in, described spray-dired temperature can be 80 DEG C ~ 200 DEG C.
In above-mentioned preparation method, step 2) in, described concentrated multiple is 0.5 times ~ 2 times.
In the present invention's application, step 2) in, the temperature of described reaction can be 60 DEG C ~ 85 DEG C, and the time of described reaction can be 1 hour ~ 3 hours.
In the present invention's application, step 2) in, the add-on of described beta-amylase and described saccharifying enzyme is 0.5% ~ 5% of system total mass, specifically can be 0.5% ~ 1.5%, 0.5% ~ 1%, 0.5%, 1% or 1.5%, described system total mass is the quality of described supernatant liquor after concentrated; The mass ratio of described beta-amylase and described saccharifying enzyme is 1:1 ~ 5, specifically can be 1:1,1:3 or 1:5; The enzyme activity of described beta-amylase and described saccharifying enzyme is 50000 ~ 100000U/g, 1g beta-amylase or saccharifying enzyme under specified conditions (60 DEG C ~ 85 DEG C, pH value be under the condition of 7 ~ 8) decompose decomposing soluble starch and produce 1mg glucose, are 1 enzyme activity unit U/g.
Transcribe as can be seen from Wheat Arabinoxylan extract of the present invention at promotion enteron aisle DNA and promote in the application test of secretion metabolism of enteron aisle, the Wheat Arabinoxylan extract of the present invention of low dose group and middle dosage group all significantly increases DNA and rna content in mouse duodenal, and the Wheat Arabinoxylan extract of the present invention of high dosage then reduces DNA and rna content in mouse duodenal.Therefore, the Wheat Arabinoxylan extract of the present invention of middle low dose group has the effect strengthening duodenal secretion metabolism, and the Wheat Arabinoxylan extract of the present invention of high dosage does not have the effect strengthening duodenal secretion metabolism.So, use Wheat Arabinoxylan extract of the present invention carry out promotion enteron aisle DNA transcribe time, can as required effective absorption dosage of the people of Wheat Arabinoxylan extract be adjusted in 0.04 ~ 0.08g/kgbw/d (purity of extract is about 60%) or 0.08 ~ 0.16g/kgbw/d (purity of extract is about 30%).
Wheat Arabinoxylan extract involved in the present invention, also has simultaneously and improves gut barrier function, promotes intestinal villi growth, reduces gut pH and increase the several functions such as enteron aisle Short-Chain Fatty Acids content.
Tool of the present invention has the following advantages:
Wheat Arabinoxylan extract of the present invention has the function promoting that enteron aisle DNA transcribes (promoting the secretion metabolism of enteron aisle), duodenal secretion metabolism can be strengthened, prepare the efficiently simple to operate of Wheat Arabinoxylan extract simultaneously, be applicable to suitability for industrialized production, and cost is lower.
Embodiment
The experimental technique used in following embodiment if no special instructions, is ordinary method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
Embodiment 1, prepare Wheat Arabinoxylan extract
The factory effluent of wheat-flour after extracting gluten powder carries out centrifugation under the condition of 3000 revs/min, gets the supernatant liquor after centrifugation; Supernatant liquor is carried out after boiling centrifugation again and obtain supernatant liquor, wherein, the temperature of boiling is 80 DEG C, and the time of boiling is 20 minutes, and the condition of centrifugation is 2000 revs/min; Namely obtain Wheat Arabinoxylan extract dry powder by spray-dried for the supernatant liquor obtained, spray-dired temperature is 80 DEG C.
The purity of Wheat Arabinoxylan extract prepared by the present embodiment is 30% (mass percentage namely containing araboxylan).
Embodiment 2, prepare Wheat Arabinoxylan extract
The factory effluent of wheat-flour after extracting gluten powder carries out centrifugation under the condition of 3000 revs/min, gets the supernatant liquor after centrifugation; Supernatant liquor is carried out after boiling centrifugation again and obtain supernatant liquor, wherein, the temperature of boiling is 100 DEG C, and the time of boiling is 70 minutes, and the condition of centrifugation is 5000 revs/min; Concentrated by the supernatant liquor obtained, concentrated multiple is 1 times; And pH value is regulated to 7.5, then add beta-amylase and saccharifying enzyme (be 1% of system total mass, and both mass ratioes are 1:1, the enzyme activity of beta-amylase is 50000U/g, and the enzyme activity of saccharifying enzyme is 50000U/g).React, the temperature of reaction is 85 DEG C, and the time of reaction is 60 minutes; Reacted reaction solution obtains supernatant liquor through centrifugal, this supernatant liquor ethanol is precipitated: the ethanol consumption of alcohol precipitation step is 35% of reaction solution for the first time, the ethanol consumption of second time alcohol precipitation step is 45% of reaction solution, obtains Wheat Arabinoxylan extract.
The purity of Wheat Arabinoxylan extract prepared by the present embodiment is 60% (mass percentage namely containing araboxylan).
Embodiment 3, prepare Wheat Arabinoxylan extract
The factory effluent of wheat-flour after extracting gluten powder and wheat starch carries out centrifugation under the condition of 3000 revs/min, gets the supernatant liquor after centrifugation; Supernatant liquor is carried out after boiling centrifugation again and obtain supernatant liquor, wherein, the temperature of boiling is 900 DEG C, and the time of boiling is 50 minutes, and the condition of centrifugation is 4000 revs/min; Concentrated by the supernatant liquor obtained, concentrated multiple is 1 times; And pH value is regulated to 7.5, then add beta-amylase and saccharifying enzyme (be 1.5% of system total mass, and both mass ratioes are 1:5, the enzyme activity of beta-amylase is 100000U/g, and the enzyme activity of saccharifying enzyme is 100000U/g).React, the temperature of reaction is 85 DEG C, and the time of reaction is 60 minutes; Reacted reaction solution obtains supernatant liquor through centrifugal, this supernatant liquor ethanol is precipitated: the ethanol consumption of alcohol precipitation step is 35% of reaction solution for the first time, the ethanol consumption of second time alcohol precipitation step is 45% of reaction solution, obtains Wheat Arabinoxylan extract.
The purity of Wheat Arabinoxylan extract prepared by the present embodiment is 60% (mass percentage namely containing araboxylan).
Embodiment 4, prepare Wheat Arabinoxylan extract
The factory effluent of wheat-flour after extracting wheat starch carries out centrifugation under the condition of 3000 revs/min, gets the supernatant liquor after centrifugation; Supernatant liquor is carried out after boiling centrifugation again and obtain supernatant liquor, wherein, the temperature of boiling is 100 DEG C, and the time of boiling is 70 minutes, and the condition of centrifugation is 5000 revs/min; Concentrated by the supernatant liquor obtained, concentrated multiple is 1 times; And pH value is regulated to 7.5, then add beta-amylase and saccharifying enzyme (be 1% of system total mass, and both mass ratioes are 1:3, the enzyme activity of beta-amylase is 100000U/g, and the enzyme activity of saccharifying enzyme is 100000U/g).React, the temperature of reaction is 85 DEG C, and the time of reaction is 60 minutes; Reacted reaction solution obtains supernatant liquor through centrifugal, this supernatant liquor ethanol is precipitated: the ethanol consumption of alcohol precipitation step is 35% of reaction solution for the first time, the ethanol consumption of second time alcohol precipitation step is 45% of reaction solution, obtains Wheat Arabinoxylan extract.
The purity of Wheat Arabinoxylan extract prepared by the present embodiment is 60% (mass percentage namely containing araboxylan).
The Wheat Arabinoxylan extract of embodiment 5, embodiment 2 preparation transcribes the application in (the secretion metabolism promoting enteron aisle) at promotion enteron aisle DNA
1, experimental technique
First for 40 kunming mices adaptability is fed basal feed and after 1 week, is divided into 4 groups at random according to body weight, often organize 10, be respectively blank group, low dose group, middle dosage group and high dose group.
The Wheat Arabinoxylan extract (being mixed with the aqueous solution) of embodiment 2 preparation of low dose group, middle dosage group and high dose group difference gavage 0.4g/kgbw/d, 0.8g/kgbw/d and 1.6g/kgbw/d.Record Mouse Weight and food ration every day, period ad lib drinking-water.
Blank group gavage water contrasts, every day gavage 0.2mL/10gbw, continuous gavage 2 weeks, every day 1 time.
Above-mentioned blank group and each tested group feed 2 weeks after, get small intestine's homogenate that 10wt% prepares in small intestine, adopt DNA and rna content in Ethidum Eremide fluorescence spectrometry small intestine
2, experimental result
In blank group and each tested group of mouse duodenal DNA and rna content as shown in table 1.
As can be seen from data in table 1, compared with blank group, in low dose group and middle dosage group mouse duodenal, DNA and rna content significantly increase (P<0.05), show that the Wheat Arabinoxylan extract of the present invention of low dosage and middle dosage enhances duodenal secretion metabolism.
Table 1 wheat araboxylan extract is on the impact of DNA in mouse duodenal and rna content
*, compared with control group, P<0.05.
1/2 of the araboxylan extract purity (60%) that araboxylan purity (30%) prepared by embodiment 1 is prepared for embodiment 2, other compositions are water soluble starch, a small amount of protein and ash content, and therefore in embodiment 1, the effective dosage ranges of extract becomes dependency with the effective dosage ranges of embodiment 2 extract.According to the results of animal in embodiment 8, known low dose group (given low 0.4g/kgbw/d) and middle dosage group (given low 0.8g/kgbw/d) have good result, supposition is learnt, namely extract gavage 0.8g/kgbw/d ~ 1.6g/kgbw/d prepared by embodiment 1 should have good result.
Extract prepared by embodiment 3 and 4 and extract effective constituent prepared by embodiment 2 are all araboxylan, purity all can reach 60%, other material water soluble starchs, a small amount of protein and general 1% ash content, so can think that extract is substantially the same in embodiment 2-4, so physiological function illustrated in above-mentioned experimentation on animals is equally applicable to the extract prepared by embodiment 3 and 4.
Claims (9)
1. Wheat Arabinoxylan extract promotes enteron aisle DNA to transcribe product in preparation or promotes the application in intestinal secretion metabolism product;
Described Wheat Arabinoxylan extract prepares according to the method comprised the steps:
The factory effluent of wheat-flour after extracting gluten powder and/or wheat starch, through centrifugation, gets supernatant liquor; Described supernatant liquor carries out centrifugation again after boiling, obtains supernatant liquor; Described supernatant liquor is through following 1) or 2) step namely obtain Wheat Arabinoxylan extract:
1) described supernatant liquor is spray-dried and get final product;
2) described supernatant liquor is through concentrated, and adjusted to ph is after 7 ~ 8, adds beta-amylase and saccharifying enzyme reacts, and obtain supernatant liquor finally by after centrifugation, described supernatant liquor, after alcohol settling, to obtain final product.
2. promote a product for the product that enteron aisle DNA transcribes or the metabolism of promotion intestinal secretion, its activeconstituents is Wheat Arabinoxylan extract;
Described Wheat Arabinoxylan extract prepares according to the method comprised the steps:
The factory effluent of wheat-flour after extracting gluten powder and/or wheat starch, through centrifugation, gets supernatant liquor; Described supernatant liquor carries out centrifugation again after boiling, obtains supernatant liquor; Described supernatant liquor is through following 1) or 2) step namely obtain Wheat Arabinoxylan extract:
1) described supernatant liquor is spray-dried and get final product;
2) described supernatant liquor is through concentrated, and adjusted to ph is after 7 ~ 8, adds beta-amylase and saccharifying enzyme reacts, and obtain supernatant liquor finally by after centrifugation, described supernatant liquor, after alcohol settling, to obtain final product.
3. application according to claim 1 or product according to claim 2, is characterized in that: described factory effluent carries out centrifugation under the condition of 1000 revs/min ~ 3000 revs/min.
4. the application according to claim 1 or 3 or the product described in Claims 2 or 3, is characterized in that: the temperature of described boiling is 80 DEG C ~ 100 DEG C, and the time of described boiling is 20 minutes ~ 70 minutes.
5. the application according to claim 1 or 3 or 4 or the product described in Claims 2 or 3 or 4, is characterized in that: the described supernatant liquor after boiling carries out centrifugation under the condition of 2000 revs/min ~ 5000 revs/min.
6. the application according to any one of claim 1 or 3-5 or the product according to any one of Claims 2 or 3-5, is characterized in that: step 1) in, the temperature of described spraying is 80 DEG C ~ 200 DEG C.
7. the application according to any one of claim 1 or 3-6 or the product according to any one of Claims 2 or 3-6, is characterized in that: step 2) in, described concentrated multiple is 0.5 ~ 2 times.
8. the application according to any one of claim 1 or 3-7 or the product according to any one of Claims 2 or 3-7, is characterized in that: step 2) in, the temperature of described reaction is 60 DEG C ~ 85 DEG C, and the time of described reaction is 1 hour ~ 3 hours.
9. the application according to any one of claim 1 or 3-8 or the product according to any one of Claims 2 or 3-8, it is characterized in that: step 2) in, the add-on of described beta-amylase and described saccharifying enzyme is 0.5% ~ 5% of system total mass, and described system total mass is the quality of described supernatant liquor after concentrated; The mass ratio of described beta-amylase and described saccharifying enzyme is 1:1 ~ 5.
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| CN201410725325.6A CN104448056A (en) | 2014-12-03 | 2014-12-03 | Application of wheat AX (arabinoxylan) extracts to promoting DNA (deoxyribonucleic acid) transcription of intestinal tracts |
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| CN201410725325.6A CN104448056A (en) | 2014-12-03 | 2014-12-03 | Application of wheat AX (arabinoxylan) extracts to promoting DNA (deoxyribonucleic acid) transcription of intestinal tracts |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103613220A (en) * | 2013-11-21 | 2014-03-05 | 中国农业科学院农产品加工研究所 | Method for extracting multiple functional components from wheat starch processing wastewater |
| CN103923219A (en) * | 2014-03-12 | 2014-07-16 | 陈影 | Production and processing technology of light phase substance pentosan of wheatmeal and wheat flour |
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- 2014-12-03 CN CN201410725325.6A patent/CN104448056A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103613220A (en) * | 2013-11-21 | 2014-03-05 | 中国农业科学院农产品加工研究所 | Method for extracting multiple functional components from wheat starch processing wastewater |
| CN103923219A (en) * | 2014-03-12 | 2014-07-16 | 陈影 | Production and processing technology of light phase substance pentosan of wheatmeal and wheat flour |
Non-Patent Citations (2)
| Title |
|---|
| HONG CHEN等: "Arabinoxylan in Wheat Is More Responsible Than Cellulose for Promoting Intestinal Barrier Function in Weaned Male Piglets", 《THE JOURNAL OF NUTRITION》 * |
| 周素梅等: "小麦中功能性多糖-阿拉伯木聚糖研究进展", 《核农学报》 * |
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