CN104397354A - Application of wheat araboxylan extract in promoting growth of intestinal villi - Google Patents
Application of wheat araboxylan extract in promoting growth of intestinal villi Download PDFInfo
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- CN104397354A CN104397354A CN201410725801.4A CN201410725801A CN104397354A CN 104397354 A CN104397354 A CN 104397354A CN 201410725801 A CN201410725801 A CN 201410725801A CN 104397354 A CN104397354 A CN 104397354A
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Abstract
The invention discloses application of a wheat araboxylan extract in promoting the growth of intestinal villi. The wheat araboxylan extract is prepared by a method comprising the following steps: performing centrifugal separation on wheat powder by production wastewater obtained by extracting gluten powder and/or wheat starch, and taking supernate, wherein the supernate is obtained by stewing and then centrifugal separation, and the wheat araboxylan extract is obtained by performing the following step (1) or (2): (1) performing spray drying to obtain the supernate, or (2) performing concentration, adjusting the pH value to be 7-8, adding beta-amylase and glucoamylase for reaction, and finally performing centrifugal separation; the supernate is subjected to ethanol precipitation to obtain the wheat araboxylan extract. The wheat araboxylan extract involved in the application also has multiple functions of enhancing the intestinal tract barrier function, promoting intestinal tract DNA transcription, reducing the pH value of the intestinal tract, increasing the content of short-chain fatty acid in the intestinal tract and the like.
Description
Technical field
The present invention relates to a kind of Wheat Arabinoxylan extract and promote the application in intestinal villi growth.
Background technology
Araboxylan (Arabinoxylans is called for short AX) is a kind of hemicellulose, is present in the nascent of plant and secondary cell wall, comprises cereal seed and timber.The polymer that AX is made up of wood sugar and arabinose two kinds sugar, also can be called pentosan.Basic structure comprises the xylan backbone and arabinose base side chain that xylose residues is formed by connecting through Isosorbide-5-Nitrae-glycosidic bond, and some arabinose also also exists the phenolic acid be connected with ester bond.
The extraction of current Wheat Arabinoxylan mainly contains two kinds of sources, and one is extract from wheat bran, and one extracts from wheat flour, and extracting method mainly contains alkaline extraction and enzyme formulation two kinds.
Wheat bran is the accessory substance of wheat processing, arabinoxylan content enriches, about more than 20%, the annual wheat bran output of China, up to more than 2,000 ten thousand tons, is being extracted from wheat bran so the research of some araboxylan extraction processes at present focuses mostly on, but araboxylan great majority in wheat bran are all water insoluble, extract difficulty, method feasible is at present alkaline extraction, and alkali is put forward technique and can be brought a large amount of containing alkali waste water discharge, causes environmental pollution.
Arabinoxylan content in flour is about 2%, wherein the extractible part of water accounts for 0.5% ~ 0.8%, extracting directly araboxylan from flour, flour material consumption is large, and extraction cost is high, only carry out small-scale at present to extract in laboratory, extracting method mostly is the centrifugal cooperation enzyme process of physics and carries out, and only for scientific research, large-scale industrial production is explored not, do not form ripe process route, can not industrialization production be carried out.
In the factory effluent of wheaten starch, Gluten, soluble arabinoxylan containing high-quality, China's wheat flour secondary industry is huge, traditional wheaten starch, Gluten processing technology be by wheat flour through water extraction with the operation such as to be separated and to obtain object, discard eluting water, often produce 1 ton of starch, the discharge capacity of high concentrated organic wastewater is between 5 ~ 12 tons, and the direct discharge of these organic wastewaters causes severe contamination to environment.Domesticly at present also without any enterprise, these processing waste waters effectively to be utilized, analyze reason, on the one hand because starch, Gu Ruan powder enterprise do not recognize the value of wash-out waste water, the using value of araboxylan in physiologically active and processing characteristics is not understood, on the other hand, there is no efficient ripe separation-extraction technology at present, cannot be used to a large amount of wash-out waste water.
The domestic research about araboxylan physiologically active focuses mostly in the araboxylan in wheat bran source, and the araboxylan physiologically active extracted from wheat flour rarely has report.Many researchers think that wheat bran may be the most effectively and the dietary fiber of the most applicable human body needs, dietary fiber composition more complicated in wheat bran, comprise cellulose, lignin, glucan, araboxylan, arabogalactan etc., specifically any or several component plays a major role, and it be unclear that.
Summary of the invention
The object of this invention is to provide a kind of Wheat Arabinoxylan extract in the application promoting intestinal villi growth or strengthen in Small Intestinal, the Wheat Arabinoxylan extract used in the present invention, extract from the organic wastewater of wheaten starch processing enterprise, Gu Ruan powder processing enterprise, the discharge of gluten powder processing enterprise, therefore the wastewater discharge of above-mentioned processing enterprise can be reduced, realize energy-saving and emission-reduction, improve its added value of product.
The application that Wheat Arabinoxylan extract provided by the invention promotes intestinal villi growth product in preparation or strengthens in Small Intestinal product;
Described Wheat Arabinoxylan extract can be prepared according to the method comprised the steps:
The factory effluent of wheat flour after extracting Gluten and/or wheaten starch, through centrifugation, gets supernatant; Described supernatant carries out centrifugation again after boiling, obtains supernatant; Described supernatant is through following 1) or 2) step namely obtain Wheat Arabinoxylan extract:
1) described supernatant is spray-dried and get final product;
2) described supernatant is through concentrated, and adjusted to ph is after 7 ~ 8, adds beta amylase and carbohydrase reacts, and obtain supernatant finally by after centrifugation, described supernatant, after alcohol settling, to obtain final product.
In the present invention's application, described factory effluent can carry out centrifugation under the condition of 1000 revs/min ~ 3000 revs/min.
In the present invention's application, the temperature of described boiling can be 80 DEG C ~ 100 DEG C, and the time of described boiling can be 20 minutes ~ 70 minutes.
In the present invention's application, the described supernatant after boiling carries out centrifugation under the condition of 2000 revs/min ~ 5000 revs/min.
In the present invention's application, step 1) in, described spray-dired temperature can be 80 DEG C ~ 200 DEG C.
In above-mentioned preparation method, step 2) in, described concentrated multiple is 0.5 times ~ 2 times.
In the present invention's application, step 2) in, the temperature of described reaction can be 60 DEG C ~ 85 DEG C, and the time of described reaction can be 1 hour ~ 3 hours.
In the present invention's application, step 2) in, the addition of described beta amylase and described carbohydrase is 0.5% ~ 5% of system gross mass, specifically can be 0.5% ~ 1.5%, 0.5% ~ 1%, 0.5%, 1% or 1.5%, described system gross mass is the quality of described supernatant after concentrated, the mass ratio of described beta amylase and described carbohydrase is 1:1 ~ 5, specifically can be 1:1,1:3 or 1:5; The enzyme activity of described beta amylase and described carbohydrase is 50000 ~ 100000U/g, 1g beta amylase or carbohydrase under specified conditions (60 DEG C ~ 85 DEG C, pH value be under the condition of 7 ~ 8) decompose decomposing soluble starch and produce 1mg glucose, are 1 enzyme activity unit U/g.
Promoting in the application test in mouse small intestine villus growth as can be seen from Wheat Arabinoxylan extract of the present invention, the Wheat Arabinoxylan extract of the present invention of low dose group and middle dosage group all significantly enhances the absorption function of small intestine, and the Wheat Arabinoxylan extract of the present invention of high dose then inhibits the absorption function of small intestine.Therefore, the Wheat Arabinoxylan extract of the present invention of middle low dose group has the effect promoting intestinal villi growth, and the Wheat Arabinoxylan extract of the present invention of high dose does not have the effect promoting intestinal villi growth.So, when using Wheat Arabinoxylan extract of the present invention to carry out the growth of promotion intestinal villi, can as required effective absorption dosage of the people of Wheat Arabinoxylan extract be adjusted in 0.04 ~ 0.08g/kgbw/d (purity of extract is about 60%) or 0.08 ~ 0.16g/kgbw/d (purity of extract is about 30%).
Wheat Arabinoxylan extract involved in the present invention, also has raising gut barrier function simultaneously, promotes that enteron aisle DNA transcribes, reduce gut pH and increase the several functions such as enteron aisle Short-Chain Fatty Acids content.
Tool of the present invention has the following advantages:
Wheat Arabinoxylan extract of the present invention has the function promoting intestinal villi growth, prepares the efficiently simple to operate of Wheat Arabinoxylan extract simultaneously, be applicable to suitability for industrialized production, and cost is lower.
Detailed description of the invention
The experimental technique used in following embodiment if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
Embodiment 1, prepare Wheat Arabinoxylan extract
The factory effluent of wheat flour after extracting Gluten carries out centrifugation under the condition of 3000 revs/min, gets the supernatant after centrifugation; Supernatant is carried out after boiling centrifugation again and obtain supernatant, wherein, the temperature of boiling is 80 DEG C, and the time of boiling is 20 minutes, and the condition of centrifugation is 2000 revs/min; Namely obtain Wheat Arabinoxylan extract dry powder by spray-dried for the supernatant obtained, spray-dired temperature is 80 DEG C.
The purity of Wheat Arabinoxylan extract prepared by the present embodiment is 30% (mass percentage namely containing araboxylan).
Embodiment 2, prepare Wheat Arabinoxylan extract
The factory effluent of wheat flour after extracting Gluten carries out centrifugation under the condition of 3000 revs/min, gets the supernatant after centrifugation; Supernatant is carried out after boiling centrifugation again and obtain supernatant, wherein, the temperature of boiling is 100 DEG C, and the time of boiling is 70 minutes, and the condition of centrifugation is 5000 revs/min; Concentrated by the supernatant obtained, concentrated multiple is 1 times; And pH value is regulated to 7.5, then add beta amylase and carbohydrase (be 1% of system gross mass, and both mass ratioes are 1:1, the enzyme activity of beta amylase is 50000U/g, and the enzyme activity of carbohydrase is 50000U/g).React, the temperature of reaction is 85 DEG C, and the time of reaction is 60 minutes; Reacted reactant liquor obtains supernatant through centrifugal, this supernatant ethanol is precipitated: the ethanol consumption of alcohol precipitation step is 35% of reactant liquor for the first time, the ethanol consumption of second time alcohol precipitation step is 45% of reactant liquor, obtains Wheat Arabinoxylan extract.
The purity of Wheat Arabinoxylan extract prepared by the present embodiment is 60% (mass percentage namely containing araboxylan).
Embodiment 3, prepare Wheat Arabinoxylan extract
The factory effluent of wheat flour after extracting Gluten and wheaten starch carries out centrifugation under the condition of 3000 revs/min, gets the supernatant after centrifugation; Supernatant is carried out after boiling centrifugation again and obtain supernatant, wherein, the temperature of boiling is 900 DEG C, and the time of boiling is 50 minutes, and the condition of centrifugation is 4000 revs/min; Concentrated by the supernatant obtained, concentrated multiple is 1 times; And pH value is regulated to 7.5, then add beta amylase and carbohydrase (be 1.5% of system gross mass, and both mass ratioes are 1:5, the enzyme activity of beta amylase is 100000U/g, and the enzyme activity of carbohydrase is 100000U/g).React, the temperature of reaction is 85 DEG C, and the time of reaction is 60 minutes; Reacted reactant liquor obtains supernatant through centrifugal, this supernatant ethanol is precipitated: the ethanol consumption of alcohol precipitation step is 35% of reactant liquor for the first time, the ethanol consumption of second time alcohol precipitation step is 45% of reactant liquor, obtains Wheat Arabinoxylan extract.
The purity of Wheat Arabinoxylan extract prepared by the present embodiment is 60% (mass percentage namely containing araboxylan).
Embodiment 4, prepare Wheat Arabinoxylan extract
The factory effluent of wheat flour after extracting wheaten starch carries out centrifugation under the condition of 3000 revs/min, gets the supernatant after centrifugation; Supernatant is carried out after boiling centrifugation again and obtain supernatant, wherein, the temperature of boiling is 100 DEG C, and the time of boiling is 70 minutes, and the condition of centrifugation is 5000 revs/min; Concentrated by the supernatant obtained, concentrated multiple is 1 times; And pH value is regulated to 7.5, then add beta amylase and carbohydrase (be 1% of system gross mass, and both mass ratioes are 1:3, the enzyme activity of beta amylase is 100000U/g, and the enzyme activity of carbohydrase is 100000U/g).React, the temperature of reaction is 85 DEG C, and the time of reaction is 60 minutes; Reacted reactant liquor obtains supernatant through centrifugal, this supernatant ethanol is precipitated: the ethanol consumption of alcohol precipitation step is 35% of reactant liquor for the first time, the ethanol consumption of second time alcohol precipitation step is 45% of reactant liquor, obtains Wheat Arabinoxylan extract.
The purity of Wheat Arabinoxylan extract prepared by the present embodiment is 60% (mass percentage namely containing araboxylan).
The Wheat Arabinoxylan extract of embodiment 5, embodiment 2 preparation is promoting the application in intestinal villi growth
1, experimental technique
First for 40 kunming mices adaptability is fed basal feed and after 1 week, is divided into 4 groups at random according to body weight, often organize 10, be respectively blank group, low dose group, middle dosage group and high dose group.
The Wheat Arabinoxylan extract (being mixed with the aqueous solution) of embodiment 2 preparation of low dose group, middle dosage group and high dose group difference gavage 0.4g/kgbw/d, 0.8g/kgbw/d and 1.6g/kgbw/d.Record Mouse Weight and food ration every day, period ad lib drinking-water.
Blank group gavage water contrasts, every day gavage 0.2mL/10gbw, continuous gavage 2 weeks, every day 1 time.
Above-mentioned blank group and each tested group feed 2 weeks after, get Treitz ligament following proximal jejunum 3 ~ 4cm intestinal segment, prolong mesenterium and cut off by intestinal tube, tiling is placed in 10wt% neutral formalin solution and fixes 3 days.Specimens paraffin embedding slices, thickness is 5 μm, and HE dyes, and at light Microscopic observation, uses observation by light microscope, utilizes IPP software to carry out random measurement intestinal villi length and Crypt depth.
2, experimental result
The side amount result of above-mentioned intestinal villi length and Crypt depth is as shown in table 1.
As can be seen from data in table 1, compared with blank group, the height of naps of low dose group and middle dosage group, enteraden height and fine hair and enteraden height ratio all significantly increase; And the height of naps of high dose group reduces, enteraden height increases, fine hair and enteraden height ratio reduce (P<0.05), this shows that the Wheat Arabinoxylan extract of the present invention of low dosage and middle dosage enhances the absorption function of small intestine, and the Wheat Arabinoxylan extract of the present invention of high dose then inhibits the absorption function of small intestine.
Table 1 Wheat Arabinoxylan extract is on the impact of mouse small intestine fine hair and enteraden
*, compared with control group, P<0.05.
1/2 of the araboxylan extract purity (60%) that araboxylan purity (30%) prepared by embodiment 1 is prepared for embodiment 2, other compositions are water soluble starch, a small amount of protein and ash content, and therefore in embodiment 1, the effective dosage ranges of extract becomes correlation with the effective dosage ranges of embodiment 2 extract.According to above-mentioned results of animal, known low dose group (given low 0.4g/kgbw/d) and middle dosage group (given low 0.8g/kgbw/d) have good result, supposition is learnt, namely extract gavage 0.8g/kgbw/d ~ 1.6g/kgbw/d prepared by embodiment 1 should have good result.
Extract prepared by embodiment 3 and 4 and extract active ingredient prepared by embodiment 2 are all araboxylan, purity all can reach 60%, other material water soluble starch, a small amount of protein and general 1% ash content, so can think that the thing extracted in embodiment 2-4 is basic the same, so physiological function illustrated in above-mentioned zoopery, be equally applicable to extract prepared by embodiment 3 and 4.
Claims (9)
1. Wheat Arabinoxylan extract promotes the application in intestinal villi growth product or enhancing Small Intestinal product in preparation;
Described Wheat Arabinoxylan extract prepares according to the method comprised the steps:
The factory effluent of wheat flour after extracting Gluten and/or wheaten starch, through centrifugation, gets supernatant; Described supernatant carries out centrifugation again after boiling, obtains supernatant; Described supernatant is through following 1) or 2) step namely obtain Wheat Arabinoxylan extract:
1) described supernatant is spray-dried and get final product;
2) described supernatant is through concentrated, and adjusted to ph is after 7 ~ 8, adds beta amylase and carbohydrase reacts, and obtain supernatant finally by after centrifugation, described supernatant, after alcohol settling, to obtain final product.
2. promote a product for the product that intestinal villi grows or enhancing Small Intestinal, its active component is Wheat Arabinoxylan extract;
Described Wheat Arabinoxylan extract prepares according to the method comprised the steps:
The factory effluent of wheat flour after extracting Gluten and/or wheaten starch, through centrifugation, gets supernatant; Described supernatant carries out centrifugation again after boiling, obtains supernatant; Described supernatant is through following 1) or 2) step namely obtain Wheat Arabinoxylan extract:
1) described supernatant is spray-dried and get final product;
2) described supernatant is through concentrated, and adjusted to ph is after 7 ~ 8, adds beta amylase and carbohydrase reacts, and obtain supernatant finally by after centrifugation, described supernatant, after alcohol settling, to obtain final product.
3. application according to claim 1 or product according to claim 2, is characterized in that: described factory effluent carries out centrifugation under the condition of 1000 revs/min ~ 3000 revs/min.
4. the application according to claim 1 or 3 or the product described in Claims 2 or 3, is characterized in that: the temperature of described boiling is 80 DEG C ~ 100 DEG C, and the time of described boiling is 20 minutes ~ 70 minutes.
5. the application according to claim 1 or 3 or 4 or the product described in Claims 2 or 3 or 4, is characterized in that: the described supernatant after boiling carries out centrifugation under the condition of 2000 revs/min ~ 5000 revs/min.
6. the application according to any one of claim 1 or 3-5 or the product according to any one of Claims 2 or 3-5, is characterized in that: step 1) in, the temperature of described spraying is 80 DEG C ~ 200 DEG C.
7. the application according to any one of claim 1 or 3-6 or the product according to any one of Claims 2 or 3-6, is characterized in that: step 2) in, described concentrated multiple is 0.5 ~ 2 times.
8. the application according to any one of claim 1 or 3-7 or the product according to any one of Claims 2 or 3-7, is characterized in that: step 2) in, the temperature of described reaction is 60 DEG C ~ 85 DEG C, and the time of described reaction is 1 hour ~ 3 hours.
9. the application according to any one of claim 1 or 3-8 or the product according to any one of Claims 2 or 3-8, it is characterized in that: step 2) in, the addition of described beta amylase and described carbohydrase is 0.5% ~ 5% of system gross mass, and described system gross mass is the quality of described supernatant after concentrated; The mass ratio of described beta amylase and described carbohydrase is 1:1 ~ 5.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103613220A (en) * | 2013-11-21 | 2014-03-05 | 中国农业科学院农产品加工研究所 | Method for extracting multiple functional components from wheat starch processing wastewater |
| CN103923219A (en) * | 2014-03-12 | 2014-07-16 | 陈影 | Production and processing technology of light phase substance pentosan of wheatmeal and wheat flour |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103613220A (en) * | 2013-11-21 | 2014-03-05 | 中国农业科学院农产品加工研究所 | Method for extracting multiple functional components from wheat starch processing wastewater |
| CN103923219A (en) * | 2014-03-12 | 2014-07-16 | 陈影 | Production and processing technology of light phase substance pentosan of wheatmeal and wheat flour |
Non-Patent Citations (1)
| Title |
|---|
| HONG CHEN ET AL: "Arabinoxylan in Wheat Is More Responsible Than Cellulose for Promoting Intestinal Barrier Function in Weaned Male Piglets", 《THE JOURNAL OF NUTRITION》 * |
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Application publication date: 20150311 |