CN104432491A - Continuous flue-cured tobacco mould inhibitor and application thereof - Google Patents
Continuous flue-cured tobacco mould inhibitor and application thereof Download PDFInfo
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- CN104432491A CN104432491A CN201410581561.5A CN201410581561A CN104432491A CN 104432491 A CN104432491 A CN 104432491A CN 201410581561 A CN201410581561 A CN 201410581561A CN 104432491 A CN104432491 A CN 104432491A
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- mould inhibitor
- tobacco
- tobacco leaf
- flue
- tobacco leaves
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
Abstract
The invention discloses a continuous flue-cured tobacco mould inhibitor. The method for preparing the continuous flue-cured tobacco mould inhibitor comprises the steps that 0.02%-0.18% of natamycin, 0.015-0.2% of nisin from streptococcus lactis and the balance water are stirred evenly and then composite biologic mould inhibitor suspension liquid is obtained; fresh mature tobacco leaves are collected and knitted to be arranged on rods and then are put into the composite biologic mould inhibitor suspension liquid, the tobacco leaves are completely immersed for 10-40 minutes, the tobacco leaves are arranged on a flue-cured tobacco chamber after being drained off, primary baking is conducted according to a conventional three-step baking technology, and the tobacco leaves are made to obtain the sustained resistance to mildewed micorganisms. The continuous flue-cured tobacco mould inhibitor is simple in technology, low in cost, good in antimould effect, and capable of continuously playing the effect of mould prevention in the full process of baking, transportation and storage of the tobacco leaves. The tobacco leaves are prevented from moulding in the primary baking, grading, transportation and storage processes, and good application and popularization value and economic benefits are achieved.
Description
Technical field
The invention belongs to technical field of cigarette processing, be specifically related to a kind of flue-cured tobacco mould inhibitor that can continue to play effect in the overall process of tobacco flue-curing, transport and storage.Meanwhile, the invention still further relates to the embody rule of described mould inhibitor in tobacco leaf processing.
Background technology
Going mouldy is by mould microbial catastrophic collapse, extensively betides in multiple field such as industry, agricultural.Tobacco leaf is through a series of processes such as just roasting, classification, purchase and packagings, surface and inside have posted a large amount of microorganism attached, because tobacco leaf hygroscopicity is strong, and containing the various nutriments required for growth of microorganism, once condition is suitable for, tobacco leaf very easily grows various microorganism in storage, decompose the organic substance in tobacco leaf, make it to go bad, mould corruption, thus make tobacco leaf occur variable color, spoiled, generate heat, the symptom such as mildew, affect exterior quality and the fragrance quality of tobacco leaf, reduce the use value of tobacco leaf.Tobacco mildew, as the global problem of tobacco, causes the attention of people day by day.
The reason of tobacco mildew comprises tobacco microorganisms monoid, tobacco leaf component, tobacco leaf self moisture and storage requirement (temperature, humidity and oxygen) four aspects.Reciprocation that is inherent and external factor determines microbial growth speed in tobacco leaf.Meanwhile, tobacco mildew also relates to complicated enzymology course of reaction.Tobacco mildew process is also not exclusively the process that micropopulation grows under restrictive condition.
For the reason of tobacco mildew, prevent tobacco mildew from mainly containing two kinds of approach.One is thoroughly killed by tobacco mildew microorganism, as adopted
60co-Y ray tobacco leaf irradiation, tobacco leaf fumigates (as hydrogen phosphide, oxirane and ozone etc.), or reaches the object of mildew-resistant with chemical bactericide etc.; Another kind is the water content by controlling tobacco leaf, the temperature of storage environment, humidity and oxygen concentration, and interpolation is chemical and biological bacteriostatic agent suppresses tobacco mildew microbial growth, reach the object reducing tobacco mildew rate and delay tobacco mildew process, as tobacco leaf controlled atmosphere storage (being filled with nitrogen and carbon dioxide), sealed oxygen-reducing (seal or add deoxidier etc. simultaneously), chemistry and biological bacteriostatic agent etc. all belong to this type of.In these methods, it is high that the storage of tobacco leaf controlled atmosphere, sealed oxygen-reducing and radiation mildew-resistant have cost, the shortcoming of complex operation; And for the tobacco leaf after just roasting, chemistry and biological (going out) microbial inoculum that presses down then exist and are difficult to add and add uneven, the shortcoming of complicated operation.
Tobacco mildew not only occurs in storage link, also can go mouldy in first roasting, the classification and transportation of tobacco leaf.Existing mold-proof method for be only the moment of tobacco leaf process, wherein in the majority with the sheet nicotinic alcohol process after junior tobacco leaf storage and beating and double roasting; Disclosed flue-cured tobacco mould inhibitor at present, its handling object is all through the junior tobacco leaf after modulation or the sheet cigarette after redrying.Because the institutional framework of fresh tobacco leaves and junior tobacco leaf or sheet cigarette is obviously different, the temperatures involved in addition in modulated process, fresh tobacco leaves is more easily subject to the damage of mould inhibitor.By literature search, not yet find by process fresh tobacco leaves, under the prerequisite not affecting tobacco leaf outward appearance and quality, the product of mildew-resistant can be carried out for tobacco flue-curing, transport and storage overall process simultaneously.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, a kind of flue-cured tobacco mould inhibitor that can continue to play mildew-proof function in the overall process of tobacco flue-curing, transport and storage is provided.
The present invention also aims to provide the embody rule of described flue-cured tobacco mould inhibitor in fresh tobacco leaf processing.
Object of the present invention is achieved by the following technical programs.
Except as otherwise noted, percentage of the present invention is percetage by weight.
A kind of flue-cured tobacco continues mould inhibitor, and be made up of the raw material of following weight percentage: by natamycin 0.02% ~ 0.18%, nisin 0.015% ~ 0.2%, all the other compositions are water, namely obtain compound bio mould inhibitor suspension after stirring.
Apply the lasting mildew-resistant ability that described mould inhibitor improves cured tobacco leaf, be specially: fresh Mature Tobacco Leaves is gathered, compile bar after put into compound bio mould inhibitor suspension, soak under complete submerged state after 10 ~ 40 minutes, one-tenth bar tobacco leaf is pulled out, In Shadely to drain, no longer drip with blade tip and be as the criterion, then fill a heatable brick bed and three ladder baking process carry out just roasting routinely, tobacco leaf can be made to obtain sustained resistance to mildew micro-organism.
Nisin (Nisin) is the peptide species Antibiotics material obtained through refining from streptococcus lactis tunning, is a kind of natural biological food preservative and antiseptic of universally acknowledged safety.Up to now, Nisin in the whole world about more than 60 countries and regions be used as anticorrisive agent.Nisin mainly suppresses most of G
+the gemma of bacterium, particularly bacterium.Nisin can suppress some bacterial classification of staphylococcus, streptococcus, Mycosphaerella and lactobacillus; Suppress the gemma of most of fusobacterium and bacillus.Such as effectively can suppress the food spoilage that clostridium botulinum, staphylococcus aureus, hemolytic streptococcus, bacillus subtilis, bacillus stearothermophilus etc. cause.Natamycin (Natamycin) also claims natamycin (Pimaricin), it is a kind of important polyene macrolides antifungal antibiotic, both various mould, saccharomycetic growth can have been suppressed, also can the generation of Antifungi toxin, be a kind of green bio anticorrisive agent of high-efficiency broad spectrum.Nisin (Nisin) and natamycin (Natamycin) are natural biological food preservative and the antiseptic of two kinds of universally acknowledged safety, be widely used in food, but have no and two kinds of anticorrisive agents are combined for the treatment of fresh tobacco leaves, undertaken by conventional three ladder baking process just roasting, make the report of tobacco leaf acquisition to the sustained resistance of microorganism.
Relative to prior art, the present invention has the following advantages:
1, the present invention can process for fresh tobacco leaves, gained mould inhibitor can not cause harmful effect to tobacco leaf outward appearance and quality, after the effect through microorganism in bake process, tobacco leaf enzyme and high temperature, mould inhibitor can not occur to decompose and interact, still maintain antimold activity, thus can continue to play mildew-proof function in the overall process of tobacco flue-curing, transport and storage.
2, nisin and natamycin coupling, can suppress the bacterium in tobacco leaf and fungi, anti-mold effect is good simultaneously, to realizing the clean storage of tobacco leaf, pushes forward safety in production comprehensively significant.
3, preparation technology of the present invention is simple, and use cost is cheap, and 1 kilogram of mould inhibitor can process the fresh tobacco leaves of 30 ~ 50 kilograms, has good application value and economic benefit.
Accompanying drawing explanation
Fig. 1 is the half leaf method experimental control figure of Application Example.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is described in further detail, but drawings and Examples are not limited to the technical solution.
Embodiment 1
By account for percentage by weight be 0.02% natamycin 200 grams, account for the nisin 150 grams that percentage by weight is 0.015%, add in the water of 999.65 kilograms, vigorous stirring is even, obtains required compound bio mould inhibitor suspension.By being numbered with an ancient wind instrument, the fresh tobacco leaves for preparing to enter barn soaks 40 minutes in compound bio mould inhibitor suspension, fill after draining a heatable brick bed routinely three ladder baking process carry out just baking, the fresh tobacco leaves of 1 kilogram of mould inhibitor process 30 kilograms.Just be transported to stockyard, Outdoor Storage 8 months after roasting end.Open buttress, 1000 grams of samples are randomly drawed from each cigarette buttress upper, middle and lower different parts, above-mentioned junior tobacco leaf and the tobacco leaf of Outdoor Storage after 8 months that with the addition of mould inhibitor, with the aspergillus fumigatus (Aspergillusfumigatus) often occurred in the tobacco leaf that goes mouldy for model bacterial classification, measure tobacco leaf to the bacteriostatic activity of mildew micro-organism with dull and stereotyped inhibition zone method.Add up the tobacco leaf gross weight of each cigarette buttress and tobacco leaf weight of going mouldy, calculate mould variability.Contrast use water replaces compound bio mould inhibitor to soak fresh tobacco leaves, and processing method is the same.
Embodiment 2
By account for percentage by weight be 0.06% natamycin 600 grams, account for the nisin 600 grams that percentage by weight is 0.06%, add in the water of 998.8 kilograms, vigorous stirring is even, obtains required compound bio mould inhibitor suspension.By being numbered with an ancient wind instrument, the fresh tobacco leaf for preparing to enter barn soaks 30 minutes in compound bio mould inhibitor suspension, fill after draining a heatable brick bed routinely three ladder baking process carry out just baking, the fresh tobacco leaves of 1 kilogram of mould inhibitor process 30 kilograms.Just be transported to stockyard, Outdoor Storage 8 months after roasting end.Open buttress, 1000 grams of samples are randomly drawed from each cigarette buttress upper, middle and lower different parts, above-mentioned junior tobacco leaf and the tobacco leaf of Outdoor Storage after 8 months that with the addition of mould inhibitor, with the aspergillus fumigatus (Aspergillusfumigatus) often occurred in the tobacco leaf that goes mouldy for model bacterial classification, measure tobacco leaf to the bacteriostatic activity of mildew micro-organism with dull and stereotyped inhibition zone method.Add up the tobacco leaf gross weight of each cigarette buttress and tobacco leaf weight of going mouldy, calculate mould variability.Contrast use water replaces compound bio mould inhibitor to soak fresh tobacco leaves, and processing method is the same.
Embodiment 3
By account for percentage by weight be 0.125% natamycin 1250 grams, account for the nisin 1250 grams that percentage by weight is 0.125%, add in the water of 997.5 kilograms, vigorous stirring is even, obtains required compound bio mould inhibitor suspension.By being numbered with an ancient wind instrument, the fresh tobacco leaf for preparing to enter barn soaks 20 minutes in compound bio mould inhibitor suspension, fill after draining a heatable brick bed routinely three ladder baking process carry out just baking, the fresh tobacco leaves of 1 kilogram of mould inhibitor process 50 kilograms.Just be transported to stockyard, Outdoor Storage 8 months after roasting end.Open buttress, 1000 grams of samples are randomly drawed from each cigarette buttress upper, middle and lower different parts, above-mentioned junior tobacco leaf and the tobacco leaf of Outdoor Storage after 8 months that with the addition of mould inhibitor, with the aspergillus fumigatus (Aspergillusfumigatus) often occurred in the tobacco leaf that goes mouldy for model bacterial classification, measure tobacco leaf to the bacteriostatic activity of mildew micro-organism with dull and stereotyped inhibition zone method.Add up the tobacco leaf gross weight of each cigarette buttress and tobacco leaf weight of going mouldy, calculate mould variability.Contrast use water replaces compound bio mould inhibitor to soak fresh tobacco leaves, and processing method is the same.
Embodiment 4
By account for percentage by weight be 0.18% natamycin 1800 grams, account for the nisin 2000 grams that percentage by weight is 0.2%, add in the water of 996.2 kilograms, vigorous stirring is even, obtains required compound bio mould inhibitor suspension.By being numbered with an ancient wind instrument, the fresh tobacco leaf for preparing to enter barn soaks 10 minutes in compound bio mould inhibitor suspension, fill after draining a heatable brick bed routinely three ladder baking process carry out just baking, the fresh tobacco leaves of 1 kilogram of mould inhibitor process 50 kilograms.Just be transported to stockyard, Outdoor Storage 8 months after roasting end.Open buttress, 1000 grams of samples are randomly drawed from each cigarette buttress upper, middle and lower different parts, above-mentioned junior tobacco leaf and the tobacco leaf of Outdoor Storage after 8 months that with the addition of mould inhibitor, with the aspergillus fumigatus (Aspergillusfumigatus) often occurred in the tobacco leaf that goes mouldy for model bacterial classification, measure tobacco leaf to the bacteriostatic activity of mildew micro-organism with dull and stereotyped inhibition zone method.Add up the tobacco leaf gross weight of each cigarette buttress and tobacco leaf weight of going mouldy, calculate mould variability.Contrast use water replaces compound bio mould inhibitor to soak fresh tobacco leaves, and processing method is the same.
The bacteriostatic experiment of embodiment 1 ~ 4 and contrast the results are shown in Table 1.
Table 1. detailed description of the invention experimental result
As shown in Table 1: fresh tobacco leaf soaks in compound bio mould inhibitor, drain after fill a heatable brick bed routinely three ladder baking process carry out just roasting, the antibacterial work of gained junior tobacco leaf to aspergillus fumigatus reaches 95%-100%; After junior tobacco leaf stores 8 months, bacteriostatic activity still remains unchanged, and illustrates that compound bio mould inhibitor is highly stable between the tobacco leaf storage life; After the junior tobacco leaf with bacteriostatic activity stores 8 months, mould variability reduces 92.1%-98.58% than contrast.Therefore, this flue-cured tobacco mould inhibitor provided by the invention and using method thereof, not only make tobacco leaf obtain sustained resistance to microorganism, and can carry out mildew-resistant for tobacco flue-curing, transport and storage overall process simultaneously, and anti-mold effect is very good.
Application Example 1
As shown in Figure 1, adopt half leaf method test, randomly draw 4000kg tobacco leaf, scratch as two halves by tobacco leaf from master pulse, half (2000kg) mould inhibitor soaks (A), and second half soaks in contrast (B) with clear water.After process, after tobacco leaf dress a heatable brick bed, three ladder baking process carry out just roasting (C) routinely, with the aspergillus fumigatus (Aspergillusfumigatus) often occurred in the tobacco leaf that goes mouldy for model bacterial classification, tobacco leaf is measured to the bacteriostatic activity (D, E) to mildew micro-organism with dull and stereotyped inhibition zone method.
Table 2. detailed description of the invention experimental result
Half leaf method (Fig. 1) is adopted to compare the tobacco leaf that with the addition of mould inhibitor and the tobacco leaf that do not add mould inhibitor, junior tobacco leaf (Fig. 1 of above-described embodiment, on E) and store the tobacco leaf (under Fig. 1, E) after 8 months and all there is resistance to aspergillus fumigatus.Both at presentation quality (Fig. 1,68), sensory evaluating smoking, tobacco conventional chemical index, flue gas conventional chemical index, thermal cracking products, non-volatile organic acids, VFA, total volatile alkaline, total aspect such as volatile acid and aroma component all without significant difference (table 2).
Claims (2)
1. flue-cured tobacco continues a mould inhibitor, is made up of the raw material of following weight percentage: by natamycin 0.02% ~ 0.18%, nisin 0.015% ~ 0.2%, and all the other compositions are water, namely obtains compound bio mould inhibitor suspension after stirring.
2. flue-cured tobacco described in claim 1 continues the application of mould inhibitor, be specially: fresh Mature Tobacco Leaves is gathered, compile bar after put into compound bio mould inhibitor suspension, soak under complete submerged state after 10 ~ 40 minutes, one-tenth bar tobacco leaf is pulled out, In Shadely to drain, no longer drip with blade tip and be as the criterion, also three ladder baking process are roasting at the beginning of carrying out routinely then to fill a heatable brick bed, namely complete process.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410581561.5A CN104432491A (en) | 2014-10-27 | 2014-10-27 | Continuous flue-cured tobacco mould inhibitor and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410581561.5A CN104432491A (en) | 2014-10-27 | 2014-10-27 | Continuous flue-cured tobacco mould inhibitor and application thereof |
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| CN104432491A true CN104432491A (en) | 2015-03-25 |
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| CN201410581561.5A Pending CN104432491A (en) | 2014-10-27 | 2014-10-27 | Continuous flue-cured tobacco mould inhibitor and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108065446A (en) * | 2016-11-12 | 2018-05-25 | 贵州嘉利达科技有限公司 | For tobacco storage mildew resistant moistureproof agent and preparation method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1255007A (en) * | 1968-04-10 | 1971-11-24 | Director General Office Of Mon | Manufacturing method for ginseng cigarettes or the like |
| RU2099993C1 (en) * | 1996-03-20 | 1997-12-27 | Кубанский государственный технологический университет | Method for preventing mold formation on tobacco leaves |
| CN101797071A (en) * | 2010-01-28 | 2010-08-11 | 川渝中烟工业公司 | Cigar mildewproof additive and using method thereof |
| CN102342583A (en) * | 2010-08-02 | 2012-02-08 | 南通烟滤嘴有限责任公司 | Filter stick containing tobacco particles and preparation method thereof |
| CN103181615A (en) * | 2013-04-03 | 2013-07-03 | 红云红河烟草(集团)有限责任公司 | Flue-cured tobacco mildew preventive |
-
2014
- 2014-10-27 CN CN201410581561.5A patent/CN104432491A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1255007A (en) * | 1968-04-10 | 1971-11-24 | Director General Office Of Mon | Manufacturing method for ginseng cigarettes or the like |
| RU2099993C1 (en) * | 1996-03-20 | 1997-12-27 | Кубанский государственный технологический университет | Method for preventing mold formation on tobacco leaves |
| CN101797071A (en) * | 2010-01-28 | 2010-08-11 | 川渝中烟工业公司 | Cigar mildewproof additive and using method thereof |
| CN102342583A (en) * | 2010-08-02 | 2012-02-08 | 南通烟滤嘴有限责任公司 | Filter stick containing tobacco particles and preparation method thereof |
| CN103181615A (en) * | 2013-04-03 | 2013-07-03 | 红云红河烟草(集团)有限责任公司 | Flue-cured tobacco mildew preventive |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108065446A (en) * | 2016-11-12 | 2018-05-25 | 贵州嘉利达科技有限公司 | For tobacco storage mildew resistant moistureproof agent and preparation method thereof |
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Application publication date: 20150325 |