CN104432106B - A kind of preparation method of degreasing fish-egg protein powder - Google Patents
A kind of preparation method of degreasing fish-egg protein powder Download PDFInfo
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- CN104432106B CN104432106B CN201410725548.2A CN201410725548A CN104432106B CN 104432106 B CN104432106 B CN 104432106B CN 201410725548 A CN201410725548 A CN 201410725548A CN 104432106 B CN104432106 B CN 104432106B
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- 238000005238 degreasing Methods 0.000 title claims abstract description 39
- 239000000843 powder Substances 0.000 title claims abstract description 31
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 22
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 50
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 25
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 25
- 102100037611 Lysophospholipase Human genes 0.000 claims abstract description 18
- 108010058864 Phospholipases A2 Proteins 0.000 claims abstract description 18
- 108090000526 Papain Proteins 0.000 claims abstract description 16
- 239000004365 Protease Substances 0.000 claims abstract description 16
- 238000000034 method Methods 0.000 claims abstract description 16
- 229940055729 papain Drugs 0.000 claims abstract description 16
- 235000019834 papain Nutrition 0.000 claims abstract description 16
- 102100031415 Hepatic triacylglycerol lipase Human genes 0.000 claims abstract description 14
- 108010013563 Lipoprotein Lipase Proteins 0.000 claims abstract description 14
- 238000000605 extraction Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 16
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 16
- 238000009835 boiling Methods 0.000 claims description 16
- 239000000463 material Substances 0.000 claims description 16
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims description 16
- 102000004190 Enzymes Human genes 0.000 claims description 15
- 108090000790 Enzymes Proteins 0.000 claims description 15
- 229940088598 enzyme Drugs 0.000 claims description 15
- 238000004108 freeze drying Methods 0.000 claims description 12
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 8
- 241000251468 Actinopterygii Species 0.000 claims description 8
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 8
- 229930003268 Vitamin C Natural products 0.000 claims description 8
- 229930003427 Vitamin E Natural products 0.000 claims description 8
- 238000010009 beating Methods 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 8
- 229910021641 deionized water Inorganic materials 0.000 claims description 8
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 8
- 230000002779 inactivation Effects 0.000 claims description 8
- 239000008347 soybean phospholipid Substances 0.000 claims description 8
- 229960003080 taurine Drugs 0.000 claims description 8
- 235000019154 vitamin C Nutrition 0.000 claims description 8
- 239000011718 vitamin C Substances 0.000 claims description 8
- 235000019165 vitamin E Nutrition 0.000 claims description 8
- 229940046009 vitamin E Drugs 0.000 claims description 8
- 239000011709 vitamin E Substances 0.000 claims description 8
- 229920001285 xanthan gum Polymers 0.000 claims description 7
- 238000001976 enzyme digestion Methods 0.000 claims description 6
- 239000011541 reaction mixture Substances 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 4
- 230000008014 freezing Effects 0.000 claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052799 carbon Inorganic materials 0.000 claims description 3
- 241000269841 Thunnus albacares Species 0.000 claims description 2
- -1 cholesteryl ester Chemical class 0.000 abstract description 4
- 125000005456 glyceride group Chemical group 0.000 abstract description 4
- 239000003960 organic solvent Substances 0.000 abstract description 4
- 150000002632 lipids Chemical class 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 210000004681 ovum Anatomy 0.000 description 3
- 235000014102 seafood Nutrition 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000000227 grinding Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 239000000376 reactant Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 238000010257 thawing Methods 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 108010044091 Globulins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108010058846 Ovalbumin Proteins 0.000 description 1
- 102000015439 Phospholipases Human genes 0.000 description 1
- 108010064785 Phospholipases Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- WORJEOGGNQDSOE-UHFFFAOYSA-N chloroform;methanol Chemical compound OC.ClC(Cl)Cl WORJEOGGNQDSOE-UHFFFAOYSA-N 0.000 description 1
- ZPUCINDJVBIVPJ-LJISPDSOSA-N cocaine Chemical compound O([C@H]1C[C@@H]2CC[C@@H](N2C)[C@H]1C(=O)OC)C(=O)C1=CC=CC=C1 ZPUCINDJVBIVPJ-LJISPDSOSA-N 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N glycerol group Chemical group OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 229940092253 ovalbumin Drugs 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 108091005981 phosphorylated proteins Proteins 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/04—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from fish or other sea animals
Abstract
The invention provides a kind of preparation method of degreasing fish-egg protein powder, its mixed enzymolysis by phospholipase A1, phospholipase A2 and papain, supercritical carbon dioxide extracting degreasing and allotment obtain degreasing fish-egg protein powder product, the method can, fully not removing glyceride, free fatty, phosphatide and the cholesteryl ester in fish-egg under condition with an organic solvent, obtain features good taste, instant and the high degreasing fish-egg protein powder product of added value.
Description
Technical field:
The present invention relates to food technology field, relate in particular to a kind of preparation method of degreasing fish-egg protein powder.
Background technology:
In the ovum of seafood fish, contain abundant lipid, vitamin A, Cobastab, vitamin D, calcium, phosphorus,The nutritional labeling such as iron, protein, and protein content accounts between the 12-33% of its weight in wet base. In fish-egg albumen, containThere are the compositions such as abundant phosphorylated protein, ovalbumin, globulin, needed by human ammonia in its amino acid compositionBase acid accounts for total amino acid more than 40%, is the good protein food sources of the mankind. But fish-egg is except minority quilt at presentBe processed into outside caviar, most fish-eggs are simply processed as the products such as animal feed, technology content and attachedValue added low, fail the protein in fish-egg to utilize fully.
Lipid component in the ovum of seafood fish comprises glyceride, free fatty, phosphatide, cholesteryl ester etc.,In long-term storage, very easily there is oxidation and produce bad gas in the polyunsaturated fatty acid that fish-egg containsTaste and to the disadvantageous oxide of health, therefore prepares the mistake of albumen powder at the ovum taking seafood fish as raw materialCheng Zhong, need to remove fat wherein conventionally.
Common degreasing method is organic solvent (as ethanol, isopropyl alcohol, n-hexane etc.) degreasing method and super facingThe method of boundary's carbon dioxide abstraction, wherein the method for organic solvent degreasing easily causes the residual of solvent in product,There is potential food safety risk; (account for total lipid content owing to containing abundant phosphatide in the total fat of fish-egg30.2%-80.3%), and carbon dioxide under supercriticality is only solid to glyceride, free fatty and courageAlcohol ester has higher solubility, and its dissolubility to phosphatide is very poor, and therefore degreasing effect is bad.
Summary of the invention:
The object of the present invention is to provide a kind of preparation method of degreasing fish-egg albumen powder, to solve prior artThe problems referred to above that exist, it obtains degreasing fish-egg protein powder product by the following method:
The first step, will thaw under freezing albacore ooecium temperature naturally, boiling water boiling 5-10 minute;
Second step, adds fish-egg weight 1-3 deionized water doubly by first step gained fish-egg, after making beating, addsPhospholipase A1, phospholipase A2 and papain carry out mixed enzymolysis under 50 DEG C of-55 DEG C of conditions;
The 3rd step, makes enzyme preparation inactivation for 15 minutes by second step gained enzyme digestion reaction mixture boiled;
The 4th step, by the 3rd step products therefrom freeze drying to moisture below 5%;
The 5th step, removes fat by the 4th step gained freeze drying product by supercritical carbon dioxide extracting,Obtain degreasing fish-egg albumen base-material;
The 6th step, gets the 5th step gained degreasing fish-egg albumen base-material 80-90 part, add xanthans 0.6-1 part,Oligoisomaltose 1-5 part, powdered soybean phospholipid 0.5-2 part and taurine 0.01-0.03 part, Ultramicro-powderBe broken to granularity 500-800 order, then add vitamin C 0.1-0.5 part, powder vitamin E 0.1-0.5 partMix and obtain finished product.
In described second step, the addition of phospholipase A1, phospholipase A2 and papain is respectively the first step0.05%-3%, 0.05%-3%, the 0.2%-2% of gained fish-egg weight.
In described second step, phospholipase A1 and phospholipase A2 are the food-grade enzyme preparation that microorganism fermentation obtains,The phosphatidase Lecitaseultra that wherein the preferred Novozymes of phospholipase A1 company provides, phospholipase A2The phosphatidase PLA2NAGASE that preferably Chang Lai enzyme preparation company provides.
In described second step, the temperature of mixed enzymolysis is 40-55 DEG C, the time 3-12 hour of enzymolysis, enzymolysisPH value be 5-8.
In described the 5th step, the condition of supercritical carbon dioxide extracting is: extracting pressure 8-40MPa, titanium dioxideThe extraction flow 20-200L/h of carbon, extraction temperature 30-60 DEG C, extraction time 4-6 hour.
The present invention also provides a kind of method of preparing degreasing fish-egg protein powder, and it comprises:
The first step, will thaw under frozen fish ooecium temperature naturally, boiling water boiling 5-10 minute;
Second step, adds fish-egg weight 1-3 deionized water doubly by first step gained fish-egg, after making beating, addsPhospholipase A1, phospholipase A2 and papain carry out mixed enzyme under 40-55 DEG C, the condition of pH5-8Separate 3-12h, the addition of described phospholipase A1, phospholipase A2 and papain is respectively first step gained0.05%-3%, the 0.05%-3% of fish-egg weight, 0.2%-2%;
The 3rd step, makes enzyme preparation inactivation for 15 minutes by second step gained enzyme digestion reaction mixture boiled;
The 4th step, by the 3rd step products therefrom freeze drying to moisture below 5%;
The 5th step, removes fat by the 4th step gained freeze drying product by supercritical carbon dioxide extracting,Obtain degreasing fish-egg albumen base-material, the condition of described supercritical carbon dioxide extracting is: extracting pressure 8-40MPa,The extraction flow 20-200L/h of carbon dioxide, extraction temperature 30-60 DEG C, extraction time 4-6 hour;
The 6th step, gets the 5th step gained degreasing fish-egg albumen base-material 80-90 part, add xanthans 0.6-1 part,Oligoisomaltose 1-5 part, powdered soybean phospholipid 0.5-2 part and taurine 0.01-0.03 part, Ultramicro-powderBe broken to granularity 500-800 order, then add vitamin C 0.1-0.5 part, powder vitamin E 0.1-0.5 partMix and obtain finished product.
The present invention also provides a kind of degreasing fish-egg protein powder, and it is prepared from by the following method:
The first step, will thaw under frozen fish ooecium temperature naturally, boiling water boiling 5-10 minute;
Second step, adds fish-egg weight 1-3 deionized water doubly by first step gained fish-egg, after making beating, addsPhospholipase A1, phospholipase A2 and papain carry out mixed enzyme under 40-55 DEG C, the condition of pH5-8Separate 3-12h, the addition of described phospholipase A1, phospholipase A2 and papain is respectively first step gained0.05%-3%, the 0.05%-3% of fish-egg weight, 0.2%-2%;
The 3rd step, makes enzyme preparation inactivation for 15 minutes by second step gained enzyme digestion reaction mixture boiled;
The 4th step, by the 3rd step products therefrom freeze drying to moisture below 5%;
The 5th step, removes fat by the 4th step gained freeze drying product by supercritical carbon dioxide extracting,Obtain degreasing fish-egg albumen base-material, the condition of described supercritical carbon dioxide extracting is: extracting pressure 8-40MPa,The extraction flow 20-200L/h of carbon dioxide, extraction temperature 30-60 DEG C, extraction time 4-6 hour;
The 6th step, gets the 5th step gained degreasing fish-egg albumen base-material 80-90 part, add xanthans 0.6-1 part,Oligoisomaltose 1-5 part, powdered soybean phospholipid 0.5-2 part and taurine 0.01-0.03 part, Ultramicro-powderBe broken to granularity 500-800 order, then add vitamin C 0.1-0.5 part, powder vitamin E 0.1-0.5 partMix and obtain finished product.
It is that catalyst is under the same conditions to fish that the present invention adopts phospholipase A1, phospholipase A2 and papainOvum carries out mixed enzymolysis, and in enzymolysis process, phospholipase A1 and phospholipase A2 respectively can be by fish-egg phosphatideTwo aliphatic acid on glycerol backbone are hydrolyzed, and obtain being easy to be dissolved in the free fat of supercritical carbon dioxideFat acid, thus the removal effect of supercritical carbon dioxide to phosphatide finally improved.
Beneficial effect
The invention provides a kind of preparation method of degreasing fish-egg protein powder, it is by phospholipase A1, phosphatideMixed enzymolysis, supercritical carbon dioxide extracting degreasing and the allotment of enzyme A2 and papain obtain degreasing fish-eggProtein powder product, the method can under condition with an organic solvent not, fully remove glyceride in fish-egg,Free fatty, phosphatide and cholesteryl ester, obtain features good taste, instant and the high degreasing fish-egg of added valueProtein powder product.
Detailed description of the invention:
Below adopt embodiment to describe embodiments of the present invention in detail, whereby how the present invention is applied to skillArt means solve technical problem, and the implementation procedure of reaching technique effect can fully understand and implement according to this.
Embodiment 1
Boiling water boiling 10 minutes after taking the freezing fish-egg of 2kg and naturally thawing under room temperature condition, pulls fish-egg out and (claimsBe heavily 1.98kg) mix making beating with 2.5kg deionized water, regulating its pH is 5.5, then adds in slurries2g phosphatidase Lecitaseultra (phospholipase A1), 3g phosphatidase PLA2NAGASE (phospholipase A2)With 10g papain, after mixing under 50 DEG C of conditions stirring reaction. React after 6 hours, will be anti-Answer mixture to be heated to seethe with excitement and be incubated 15 minutes enzyme preparation inactivations fully to make to add, by reactant mixtureFreeze drying is extracted in still to adding after water content 3%, temperature 50 C, carbon dioxide flow velocity 100L/h,Under the condition of pressure 33MPa with supercritical carbon dioxide extracting 4 hours, obtain total lipid content and be 0.4% (byChloroform methanol method measure) fish-egg protein powder base-material 312.4g, get this base-material 240g add xanthans 2g,Oligoisomaltose 9g, powdered soybean phospholipid 3g and taurine 0.05g, ultramicro grinding is to granularity 500-800Order, then add vitamin C 0.4g and powder vitamin E 0.5g to mix and obtain degreasing fish-egg proteinPowder product 250.4g.
Embodiment 2
Boiling water boiling 10 minutes after taking the freezing fish-egg of 8kg and naturally thawing under room temperature condition, pulls fish-egg out and (claimsBe heavily 7.5kg) mix making beating with 8kg deionized water, regulating its pH is 5.5, then adds 10g in slurriesPhosphatidase Lecitaseultra (phospholipase A1), 10g phosphatidase PLA2NAGASE (phospholipase A2) and40g papain, after mixing under 55 DEG C of conditions stirring reaction. React after 8 hours, will reactMixture is heated to seethe with excitement and is incubated 15 minutes enzyme preparation inactivations fully to make to add, and reactant mixture is coldFreeze to be dried to after water content 3.4% and add and extract in still, temperature 50 C, carbon dioxide flow velocity 105L/h,Under the condition of pressure 31MPa, use supercritical carbon dioxide extracting 5 hours, obtaining total lipid content is 0.34%The fish-egg protein powder base-material 630.8g of (being measured by chloroform methanol method), gets this base-material 240g and adds xanthans2.4g, oligoisomaltose 6g, powdered soybean phospholipid 3g and taurine 0.03g, ultramicro grinding is to granularity500-800 order, then add vitamin C 0.8g, powder vitamin E 0.9g to mix to obtain finished product 251.2g.
The above, be only preferred embodiment of the present invention, not the present invention made to other formRestriction, any those skilled in the art may utilize the technology contents of above-mentioned announcement changed or changeType is the equivalent embodiment of equivalent variations. But every technical solution of the present invention content that do not depart from, according to thisAny simple modification, equivalent variations and remodeling that bright technical spirit is done above embodiment, still belong to thisThe protection domain of invention technical scheme.
Claims (6)
1. a preparation method for degreasing fish-egg protein powder, is characterized in that, described method comprises:
The first step, will thaw under freezing albacore ooecium temperature naturally, boiling water boiling 5-10 minute;
Second step, adds fish-egg weight 1-3 deionized water doubly by first step gained fish-egg, after making beating, addsPhospholipase A1, phospholipase A2 and papain carry out mixed enzymolysis under 50 DEG C of-55 DEG C of conditions;
The 3rd step, makes enzyme preparation inactivation for 15 minutes by second step gained enzyme digestion reaction mixture boiled;
The 4th step, by the 3rd step products therefrom freeze drying to moisture below 5%;
The 5th step, removes fat by the 4th step gained freeze drying product by supercritical carbon dioxide extracting,Obtain degreasing fish-egg albumen base-material;
The 6th step, gets the 5th step gained degreasing fish-egg albumen base-material 80-90 part, add xanthans 0.6-1 part,Oligoisomaltose 1-5 part, powdered soybean phospholipid 0.5-2 part and taurine 0.01-0.03 part, Ultramicro-powderBe broken to granularity 500-800 order, then add vitamin C 0.1-0.5 part, powder vitamin E 0.1-0.5 partMix and obtain finished product.
2. the preparation method of a kind of degreasing fish-egg protein powder as claimed in claim 1, is characterized in that,In described second step, the addition of phospholipase A1, phospholipase A2 and papain is respectively first step gained fish0.05%-3%, the 0.05%-3% of egg weight, 0.2%-2%.
3. the preparation method of a kind of degreasing fish-egg protein powder as claimed in claim 1, is characterized in that,In described second step, the temperature of mixed enzymolysis is 40-55 DEG C, the time 3-12 hour of enzymolysis, the pH value of enzymolysisFor 5-8.
4. the preparation method of a kind of degreasing fish-egg protein powder as claimed in claim 1, is characterized in that,In described the 5th step, supercritical carbon dioxide extracting removes fatty condition and is: extracting pressure 8-40MPa, dioxyChange the extraction flow 20-200L/h of carbon, extraction temperature 30-60 DEG C, extraction time 4-6 hour.
5. a preparation method for degreasing fish-egg protein powder, is characterized in that, comprising:
The first step, will thaw under frozen fish ooecium temperature naturally, boiling water boiling 5-10 minute;
Second step, adds fish-egg weight 1-3 deionized water doubly by first step gained fish-egg, after making beating, addsPhospholipase A1, phospholipase A2 and papain carry out mixed enzyme under 40-55 DEG C, the condition of pH5-8Separate 3-12h, the addition of described phospholipase A1, phospholipase A2 and papain is respectively first step gained0.05%-3%, the 0.05%-3% of fish-egg weight, 0.2%-2%;
The 3rd step, makes enzyme preparation inactivation for 15 minutes by second step gained enzyme digestion reaction mixture boiled;
The 4th step, by the 3rd step products therefrom freeze drying to moisture below 5%;
The 5th step, removes fat by the 4th step gained freeze drying product by supercritical carbon dioxide extracting,Obtain degreasing fish-egg albumen base-material, the condition of described supercritical carbon dioxide extracting is: extracting pressure 8-40MPa,The extraction flow 20-200L/h of carbon dioxide, extraction temperature 30-60 DEG C, extraction time 4-6 hour;
The 6th step, gets the 5th step gained degreasing fish-egg albumen base-material 80-90 part, add xanthans 0.6-1 part,Oligoisomaltose 1-5 part, powdered soybean phospholipid 0.5-2 part and taurine 0.01-0.03 part, Ultramicro-powderBe broken to granularity 500-800 order, then add vitamin C 0.1-0.5 part, powder vitamin E 0.1-0.5 partMix and obtain finished product.
6. a degreasing fish-egg protein powder, is characterized in that, described degreasing fish-egg protein powder be by asBelow method is prepared from:
The first step, will thaw under frozen fish ooecium temperature naturally, boiling water boiling 5-10 minute;
Second step, adds fish-egg weight 1-3 deionized water doubly by first step gained fish-egg, after making beating, addsPhospholipase A1, phospholipase A2 and papain carry out mixed enzyme under 40-55 DEG C, the condition of pH5-8Separate 3-12h, the addition of described phospholipase A1, phospholipase A2 and papain is respectively first step gained0.05%-3%, the 0.05%-3% of fish-egg weight, 0.2%-2%;
The 3rd step, makes enzyme preparation inactivation for 15 minutes by second step gained enzyme digestion reaction mixture boiled;
The 4th step, by the 3rd step products therefrom freeze drying to moisture below 5%;
The 5th step, removes fat by the 4th step gained freeze drying product by supercritical carbon dioxide extracting,Obtain degreasing fish-egg albumen base-material, the condition of described supercritical carbon dioxide extracting is: extracting pressure 8-40MPa,The extraction flow 20-200L/h of carbon dioxide, extraction temperature 30-60 DEG C, extraction time 4-6 hour;
The 6th step, gets the 5th step gained degreasing fish-egg albumen base-material 80-90 part, add xanthans 0.6-1 part,Oligoisomaltose 1-5 part, powdered soybean phospholipid 0.5-2 part and taurine 0.01-0.03 part, Ultramicro-powderBe broken to granularity 500-800 order, then add vitamin C 0.1-0.5 part, powder vitamin E 0.1-0.5 partMix and obtain finished product.
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| CN104855832B (en) * | 2015-06-05 | 2018-07-13 | 福建农林大学 | A kind of fortification oatmeal food and preparation method thereof of the albumen of roe containing Larimichthys crocea |
| CN105831388A (en) * | 2015-12-15 | 2016-08-10 | 浙江海洋学院 | Preparation method of taurine-rich protein powder |
| GB201522681D0 (en) * | 2015-12-22 | 2016-02-03 | Dupont Nutrition Biosci Aps | Composition |
| CN107252060A (en) * | 2017-05-27 | 2017-10-17 | 舟山达康科技有限公司 | A kind of processing method of low value eupleurogrammus muticus fish meal |
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