CN102048020B - Method for extracting yellow mealworm active proteins by using alkali-enzyme method - Google Patents
Method for extracting yellow mealworm active proteins by using alkali-enzyme method Download PDFInfo
- Publication number
- CN102048020B CN102048020B CN 200910044692 CN200910044692A CN102048020B CN 102048020 B CN102048020 B CN 102048020B CN 200910044692 CN200910044692 CN 200910044692 CN 200910044692 A CN200910044692 A CN 200910044692A CN 102048020 B CN102048020 B CN 102048020B
- Authority
- CN
- China
- Prior art keywords
- worm
- extraction
- yellow mealworm
- drying
- yellow meal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a method for extracting yellow mealworm active proteins by using an alkali-enzyme method, comprising the following steps: drying fresh yellow mealworm larvae to obtain dried yellow mealworm larvae of which the moisture content is lower than 5%, and smashing the dried yellow mealworm larvae; carrying out secondary digestion by taking petroleum ether of which the relative density is 0.64-0.66 as an extraction solvent; carrying out digestion with a 0.08-0.10mol/L NaOH solution; regulating the pH value to 7.0-7.5, carrying out enzymatically hydrolyzed extraction at a temperature of 55-60 DEG C by complex enzyme which is composed of neutral protease and papain at the mass ratio of 1:1-1:1.5; destroying enzyme; and centrifuging and dialyzing to obtain yellow mealworm protein powder. The yellow mealworm protein powder has the advantages of light yellow color and luster, beauty, fragrant odor as well as fine and smooth taste; and the protein content of the yellow mealworm protein powder reaches 94.5% and the extraction efficiency of the yellow mealworm protein powder reaches 75%.
Description
Technical field:
The present invention relates to field of food, relate in particular to a kind of deep process technology that adopts the alkali Enzymatic Extraction to can be used for the yellow mealworm protein matter of food production.
Background technology:
Yellow meal worm (Tenebrio molitor.Linne) has another name called: the bloom first, be commonly called as the bread worm, belong to Insecta; Coleoptera, Tenebrionidae, mealworm belongs to; Be distributed widely in the province such as Heilungkiang, Liaoning, Gansu, Sichuan, Shandong, Hubei of China, its growth cycle is 1 year generation or two generations.Yellow meal worm has rich nutrient contents, and delicious flavour is a kind of protein resource that is worth development and use.Its fresh larva contains yellow mealworm protein content and is about 68.5%, fat 18.9%, and inorganic salts 1.45%, vitamin 0.20%, other are 10.95% years old.Yellow meal worm amino acid A wide selection of colours and designs; And contain necessary 18 kinds of essential amino acids of human body and histidine; Its essential amino acids content accounts for 39.14% of total amino acid content, and the good protein essential amino acid that reaches the FAO/WHO suggestion basically should account for the standard of total amino acid content 40%.For a long time, because it is lacked systematic research, therefore develop lessly, quite a few makes a large amount of nutritional resources be wasted as feed.
Summary of the invention:
Technical problem to be solved by this invention is: be directed against on the basis that Enzymatic Extraction protein is succeeded in developing in the above-mentioned prior art, for high yield, requirement of high purity, a kind of method of alkali Enzymatic Extraction yellow meal worm activated protein be provided in conjunction with the food protein exploitation.
In order to solve the problems of the technologies described above, the technical scheme that the present invention adopted is: a kind of method of alkali Enzymatic Extraction yellow meal worm activated protein, and this method comprises the step of following order:
A, drying: it is dry to get fresh Yellow meal worm larva, obtains water content the dried worm of the yellow meal worm below 5%; Wherein, dry is freeze drying or forced air drying.Freeze drying be with fresh Yellow meal worm larva at-15 ℃ of pre-freeze 10h～15h, then vacuum be 100Pa, condenser temperature for-50 ℃ under, continuous drying 24h.Forced air drying be with fresh Yellow meal worm larva in 55～60 ℃ of following dry 54h, also can obtain water content smaller or equal to 5% yellow meal worm dry powder.
B, degreasing: the dried worm of drying is pulverized; With relative density is that 0.64～0.66 benzinum serves as to leach solvent, adds the ratio of 3～5ml benzinum in 1g worm powder, and the adding benzinum carries out the secondary lixiviate in the worm powder; Extraction temperature is 60～80 ℃, and each extraction time is 2～3h; It is low as far as possible to expect that the high yellow meal worm active protein powder of protein content just must control to the fatty residual quantity of worm powder, generally is controlled at protein content is reached more than 90%.Once leach residual oil content below 10%, secondary leaches residual oil content below 5%, leaches through secondary to reach the degreasing requirement basically.
C, carry in advance: the yellow meal worm dry powder after dry, degreasing, with the NaOH solution of 0.08～0.10mol/L, restrain the ratio lixiviate that yellow meal worm dry powder adds 6～8mlNaOH solution in every, extraction time is 1～2h, 45～50 ℃ of extraction temperatures;
D, enzyme-added extraction: adjust pH is 7.0～7.5; Under 55～60 ℃; Extract in the ratio that every 100g worm powder adds 2～3g complex enzyme with complex enzyme; Enzymolysis and extraction time 120～150min, complex enzyme is made up of neutral proteinase and papain, and the mass ratio of neutral proteinase and papain is 1: 1～1: 1.5;
E, enzyme goes out: will go up the hydrolyzate that the step obtains and be heated to 90 ℃, and keep 5～10min enzyme that goes out;
F, centrifugal, dialysis: the centrifuging and taking supernatant with the deionized water 12h that dialyses, promptly gets the yellow mealworm protein powder with the hydrolyzate spray-drying then under 4 ℃.Wherein, centrifugal rotational speed is 5500r/min, and the time is 3～5min.Spray-drying is a prior art, repeats no more at this, but comes the report of product among dry the present invention not see as yet with this method.
Aspect control product recovery rate, proposed elder generation and extracted with sig water, select for use two kinds of compound interpolations of protease of neutral proteinase and papain to carry out the extraction of yellow mealworm protein matter then.The invention provides the approach of a kind of yellow mealworm protein matter as the food deep processing, whole technology is simple, and investment goods is few, and the yield of product and purity are high, and product quality is easy to control, is fit to very much practice and promotes.
The invention has the beneficial effects as follows, adopt freeze-drying method that raw material is carried out drying, can fully keep the active component of insect protein; Degreasing uses benzinum for leaching solvent, carries out can reducing resid amount greatly after the secondary lixiviate; At first carry yellow mealworm protein in advance, select for use the compound extraction of neutral proteinase and papain can greatly improve the recovery rate of albumen then with sig water; Dialysis can be removed because of regulating P H and changed salt and other small-molecule substances that produces.Drying means is selected spray-drying for use, and this method sample heated time is short, can well keep the activity of yellow mealworm protein.It is yellowish, attractive in appearance to produce the products obtained therefrom color and luster by above-mentioned processing step, and smell fragrance, delicate mouthfeel, protein content is up to 94.5%, extraction rate reached to 75%.
The specific embodiment:
Embodiment 1:
Getting fresh Yellow meal worm larva 100g and put into freeze drier in-15 ℃ of pre-freeze 10h, is that 100Pa, condenser temperature are that continuous drying 24h promptly obtains the dried worm of yellow meal worm, and is subsequent use under-50 ℃ the condition in vacuum again.With relative density is that 0.64～0.66 benzinum serves as to leach solvent, and the ratio that adds the 3ml benzinum in 1g worm powder adds benzinum, carries out the secondary lixiviate, and extraction temperature is 60 ℃, and each extraction time is 2h, yellow meal worm degreasing dry powder.To with the NaOH solution of 0.08mol/L, under 45 ℃, carry 1h in advance through the yellow meal worm dry powder after freeze drying, the degreasing in the ratio that every gram worm powder adds 6mlNaOH solution.Then, regulating pH value is 7.0, under 55 ℃, adds neutral proteinase and the papain of 2.0g, and the mass ratio of neutral proteinase and papain is 1: 1, behind the extraction time 120min, is warming up to 90 ℃, maintenance 5～10min enzyme that goes out; Centrifuging and taking supernatant under 5000r/min * 3min condition again, 4 ℃ with the deionized water 12h that dialyses, and at last hydrolyzate is carried out spray-drying, promptly gets yellow meal worm active protein powder 49.2g.Yellow meal worm activated protein recovery rate is 75.6%, and protein content is 95.2%, and fat content is 1.20%, and moisture contains 5%.
Embodiment 2:
Get fresh Yellow meal worm larva 100g and put into before the freeze drier in-15 ℃ of pre-freeze 15h, again vacuum be 100Pa, condenser temperature under-50 ℃ the condition, continuous drying 24h promptly obtains the dried worm of yellow meal worm, and is subsequent use.With the benzinum is to leach solvent, adds 500ml benzinum (relative density is 0.64～0.66, and is pure for analyzing) and carries out the secondary lixiviate, and extraction temperature is 80 ℃, and each extraction time is 3h, gets yellow meal worm degreasing dry powder.To with the NaOH solution of 0.10mol/L, under 50 ℃, carry 2h in advance through the yellow meal worm dry powder after freeze drying, the degreasing in the ratio that every gram worm powder adds 8ml NaOH solution.Then, regulating pH value is 7.5, under 60 ℃, adds neutral proteinase and the papain of 3.0g, and the mass ratio of neutral proteinase and papain is 1: 1.5, behind the extraction time 150min, is warming up to 90 ℃, is incubated 5～10min enzyme that goes out; Centrifuging and taking supernatant under 5000r/min * 5min condition again, 4 ℃ with the deionized water 12h that dialyses, and at last hydrolyzate is carried out spray-drying, promptly gets yellow meal worm active protein powder 49.5g.Yellow meal worm activated protein recovery rate is 76.15%, and protein content is 94.5%, and fat content is 1.0%, and moisture is 5.5%.
The present invention adopts the foregoing description products obtained therefrom to compare with traditional product, and the result sees the following form 1:
| Product 1 of the present invention | Product 2 of the present invention | Traditional product | |
| Color and luster | Color and luster is yellowish, even | Color and luster is yellowish, even | Color and luster is deep yellow, even |
| Mouthfeel | Exquisiteness, no strange taste | Exquisiteness, no strange taste | Rough mouthfeel is arranged, a little fishy smell |
| Smell | The distinctive fragrance of yellow mealworm protein is arranged | The distinctive fragrance of yellow mealworm protein is arranged | Light fishy smell |
| The form tissue | Powdered and even | Powdered and even | Powdered but inhomogeneous, there is particle to exist |
Can be known and the foregoing description can be known that by last table the protein content of product of the present invention is up to 94.5%, recovery rate is higher than 75%, and fat content is lower than 2%, and moisture is lower than 6%.And the organoleptic indicator of product of the present invention all will be higher than traditional product, and the microbiological indicator of product of the present invention is that microorganism and other sanitary index meet the drug standards.
Claims (3)
1. the method for an alkali Enzymatic Extraction yellow meal worm activated protein, it is characterized in that: this method comprises the step of following order:
A, drying: get fresh Yellow meal worm larva freeze drying, obtain water content and be lower than 5% the dried worm of yellow meal worm;
B, degreasing: the dried worm of drying is pulverized; With relative density is that 0.64～0.66 benzinum serves as to leach solvent, adds the ratio of 3～5ml benzinum in 1g worm powder, and the adding benzinum carries out the secondary lixiviate in the worm powder; Extraction temperature is 60～80 ℃, and each extraction time is 2～3h;
C, carry in advance: the worm powder after dry, degreasing, with the NaOH solution of 0.08～0.10mol/L, restrain the ratio lixiviate that the worm powder adds 6～8mlNaOH solution in every, extraction time is 1～2h, 45～50 ℃ of extraction temperatures;
D, enzyme-added extraction: adjust pH is 7.0～7.5; Under 55～60 ℃; Extract in the ratio that every 100g worm powder adds 2～3g complex enzyme with complex enzyme; Enzymolysis and extraction time 120～150min, complex enzyme is made up of neutral proteinase and papain, and the mass ratio of neutral proteinase and papain is 1: 1～1: 1.5;
E, enzyme goes out: will go up the hydrolyzate that the step obtains and be heated to 90 ℃, and keep 5～10min;
F, centrifugal, dialysis: the centrifuging and taking supernatant with the deionized water 12h that dialyses, promptly gets the yellow mealworm protein powder with the hydrolyzate spray-drying then under 4 ℃.
2. the method for alkali Enzymatic Extraction yellow meal worm activated protein as claimed in claim 1; It is characterized in that: said freeze drying is in-15 ℃ of pre-freeze 10h～15h with fresh Yellow meal worm larva; Be under 100Pa, condenser temperature are-50 ℃ in vacuum then, continuous drying 24h.
3. the method for alkali Enzymatic Extraction yellow meal worm activated protein as claimed in claim 1 is characterized in that: the centrifugal rotational speed in the said f step is 5500r/min, and the time is 3～5min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910044692 CN102048020B (en) | 2009-11-04 | 2009-11-04 | Method for extracting yellow mealworm active proteins by using alkali-enzyme method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910044692 CN102048020B (en) | 2009-11-04 | 2009-11-04 | Method for extracting yellow mealworm active proteins by using alkali-enzyme method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102048020A CN102048020A (en) | 2011-05-11 |
| CN102048020B true CN102048020B (en) | 2012-12-05 |
Family
ID=43953089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910044692 Expired - Fee Related CN102048020B (en) | 2009-11-04 | 2009-11-04 | Method for extracting yellow mealworm active proteins by using alkali-enzyme method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102048020B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321147B (en) * | 2011-09-08 | 2013-06-12 | 金振喜 | Method for extracting tenebrio molitor protein of fiber for producing wig |
| NL2009044C2 (en) * | 2012-06-21 | 2013-12-24 | Protix Biosystems B V | Method to convert insects or worms into nutrient streams and compositions obtained thereby. |
| CN102697019B (en) * | 2012-06-25 | 2014-08-06 | 天津春发生物科技集团有限公司 | Meaty reaction essence prepared by using enzymatic hydrolysate for larvae of barley pests |
| FR3031115B1 (en) | 2014-12-31 | 2018-03-02 | Ynsect | PRODUCTION OF CHITINES FROM INSECTS BY ENZYMATIC HYDROLYSIS COMPRISING A COMBINATION OF PRELIMINARY STEPS |
| FR3031113B1 (en) | 2014-12-31 | 2018-03-16 | Ynsect | PROCESS FOR PRODUCTION OF PRODUCT (S) OF INTEREST FROM INSECT BY ENZYMATIC HYDROLYSIS |
| CN106819402A (en) * | 2017-01-20 | 2017-06-13 | 重庆科技学院 | Feed insect albumen powder production technology |
| CN109371090A (en) * | 2018-12-25 | 2019-02-22 | 浙江鼎益生物科技有限公司 | A kind of process for extracting bread worm protein |
| CN113563411A (en) * | 2021-08-25 | 2021-10-29 | 青岛新农康源生物工程有限公司 | Tenebrio molitor protein extraction process |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101124936A (en) * | 2007-08-06 | 2008-02-20 | 杨琳 | Method for preparing yellow mealworm protein |
| CN101204230A (en) * | 2006-12-21 | 2008-06-25 | 陈德国 | Further processing for tenebrio molitor and uses thereof |
| CN101411405A (en) * | 2008-11-26 | 2009-04-22 | 中国农业大学 | Insect tissue zymolyte and use thereof in artificial culture of entomopathogenic nematodes |
-
2009
- 2009-11-04 CN CN 200910044692 patent/CN102048020B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101204230A (en) * | 2006-12-21 | 2008-06-25 | 陈德国 | Further processing for tenebrio molitor and uses thereof |
| CN101124936A (en) * | 2007-08-06 | 2008-02-20 | 杨琳 | Method for preparing yellow mealworm protein |
| CN101411405A (en) * | 2008-11-26 | 2009-04-22 | 中国农业大学 | Insect tissue zymolyte and use thereof in artificial culture of entomopathogenic nematodes |
Non-Patent Citations (3)
| Title |
|---|
| 刘汉灵 等.黄粉虫蛋白抗菌肽酶解工艺研究.《食品科学》.2009,第30卷(第15期),156-159. * |
| 张莉莎 等.双酶水解黄粉虫蛋白制备生物活性肽的工艺优化.《西北农林科技大学学报》.2009,第37卷(第2期),165-172. * |
| 胡荣学 等.酶法制备黄粉虫蛋白粉工艺研究.《中国酿造》.2008,(第24期),94-96. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102048020A (en) | 2011-05-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102048020B (en) | Method for extracting yellow mealworm active proteins by using alkali-enzyme method | |
| CN101874590B (en) | Chicken bone protein zymolyte and chicken essence substrate prepared by same | |
| CN103815141B (en) | Folium Eucommiae fodder additives and its preparation method | |
| CN104286247A (en) | Preparation method of detoxified gingko tea | |
| CN106072419B (en) | A kind of compound meaty flavoring of Litsea pungens flavor and preparation method thereof | |
| CN105285925A (en) | Seasoning sauce for flavor chicken and preparation method thereof | |
| CN104161157A (en) | Globe artichoke tea and preparation method thereof | |
| CN103976411B (en) | A kind of manufacture method of product of delivering vegetables | |
| CN103598358A (en) | Production method for fermentation type hippophae rhamnosides leaf tea | |
| CN102618522A (en) | Method for industrially producing nattokinase by using chickpea | |
| CN101564149B (en) | Preparation of duck egg sauce | |
| CN102816667B (en) | Ganoderma lucidum polysaccharide nutrition sweet wine and production process | |
| CN106262578A (en) | A kind of method preparing bone soup seasoning powder | |
| CN103202451B (en) | Preparation method of mushroom sauce added with nano-calcium powder | |
| CN103800422B (en) | Compound electuary preparation of eucommia health care and products thereof | |
| CN102524738A (en) | Method for producing composite baste through mixed solid state fermentation | |
| CN103504257B (en) | Preparation method of low molecular weight mutton flavor peptide base material | |
| CN104223004A (en) | Processing method of nori soy sauce | |
| KR102147881B1 (en) | Manufacturing method of functional ginseng soy sauce fused with zinc and selenium | |
| CN102987330B (en) | Method for producing flavoring material by double-bacteria fermentation bone paste | |
| CN104256512A (en) | Manufacturing method of oyster soybean sauce | |
| KR102147880B1 (en) | Manufacturing method of functional ginseng toenjang fused with zinc and selenium | |
| CN103798520B (en) | A kind of feed additive of substitute antibiotics and preparation method thereof | |
| CN113498836A (en) | Cordyceps sinensis and eucommia ulmoides beverage and preparation method thereof | |
| KR20130084441A (en) | Making method of fucoidan from the root extract of tangleweed |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address | ||
| CP03 | Change of name, title or address |
Address after: Tongzi high tech Zone of Hunan province Changsha slope 410205 West Road No. 229 Lu Lugu International Industrial Park building 408-1 research branch Patentee after: Hunan Saibang Biological Technology Co. Ltd. Address before: 410006 a unit 202 room, women's compound courtyard, Jinxing Road, Changsha, Hunan, Yuelu District Patentee before: Changsha Saibang Biotechnology Co., Ltd. |
|
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121205 Termination date: 20181104 |